LOCUS       DAYYGY010000001       121108 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000001 DAYYGY010000000
VERSION     DAYYGY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 121108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 121108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..121108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.001"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..2122)
                     /gene="pknB"
                     /locus_tag="ACHA52_000001"
     CDS             complement(53..2122)
                     /gene="pknB"
                     /locus_tag="ACHA52_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8938766.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(2119..2859)
                     /locus_tag="ACHA52_000002"
     CDS             complement(2119..2859)
                     /locus_tag="ACHA52_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8938767.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(2872..4230)
                     /gene="rsmB"
                     /locus_tag="ACHA52_000003"
     CDS             complement(2872..4230)
                     /gene="rsmB"
                     /locus_tag="ACHA52_000003"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8938768.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(4230..5168)
                     /gene="fmt"
                     /locus_tag="ACHA52_000004"
     CDS             complement(4230..5168)
                     /gene="fmt"
                     /locus_tag="ACHA52_000004"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8938769.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(5161..5652)
                     /gene="def"
                     /locus_tag="ACHA52_000005"
     CDS             complement(5161..5652)
                     /gene="def"
                     /locus_tag="ACHA52_000005"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8938770.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(5667..8075)
                     /gene="priA"
                     /locus_tag="ACHA52_000006"
     CDS             complement(5667..8075)
                     /gene="priA"
                     /locus_tag="ACHA52_000006"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8938771.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(8328..8633)
                     /gene="rpoZ"
                     /locus_tag="ACHA52_000007"
     CDS             complement(8328..8633)
                     /gene="rpoZ"
                     /locus_tag="ACHA52_000007"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8938772.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(8638..9252)
                     /gene="gmk"
                     /locus_tag="ACHA52_000008"
     CDS             complement(8638..9252)
                     /gene="gmk"
                     /locus_tag="ACHA52_000008"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8938773.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(9739..10023)
                     /locus_tag="ACHA52_000009"
     CDS             complement(9739..10023)
                     /locus_tag="ACHA52_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938774.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(10041..10931)
                     /locus_tag="ACHA52_000010"
     CDS             complement(10041..10931)
                     /locus_tag="ACHA52_000010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8938775.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(10951..11169)
                     /locus_tag="ACHA52_000011"
     CDS             complement(10951..11169)
                     /locus_tag="ACHA52_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8938776.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(11139..12839)
                     /locus_tag="ACHA52_000012"
     CDS             complement(11139..12839)
                     /locus_tag="ACHA52_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8938777.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            13105..13878
                     /locus_tag="ACHA52_000013"
     CDS             13105..13878
                     /locus_tag="ACHA52_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938778.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(13959..14528)
                     /gene="thiT"
                     /locus_tag="ACHA52_000014"
     CDS             complement(13959..14528)
                     /gene="thiT"
                     /locus_tag="ACHA52_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8938779.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(14594..14696)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14702..15673)
                     /locus_tag="ACHA52_000015"
     CDS             complement(14702..15673)
                     /locus_tag="ACHA52_000015"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8938780.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(15767..17140)
                     /gene="glmU"
                     /locus_tag="ACHA52_000016"
     CDS             complement(15767..17140)
                     /gene="glmU"
                     /locus_tag="ACHA52_000016"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8938781.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEAFFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(17643..18461)
                     /gene="purR"
                     /locus_tag="ACHA52_000017"
     CDS             complement(17643..18461)
                     /gene="purR"
                     /locus_tag="ACHA52_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8938782.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(18628..19452)
                     /locus_tag="ACHA52_000018"
     CDS             complement(18628..19452)
                     /locus_tag="ACHA52_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8938783.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(19425..20105)
                     /locus_tag="ACHA52_000019"
     CDS             complement(19425..20105)
                     /locus_tag="ACHA52_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8938784.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(20128..21066)
                     /locus_tag="ACHA52_000020"
     CDS             complement(20128..21066)
                     /locus_tag="ACHA52_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8938785.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(21311..22162)
                     /gene="ispE"
                     /locus_tag="ACHA52_000021"
     CDS             complement(21311..22162)
                     /gene="ispE"
                     /locus_tag="ACHA52_000021"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8938786.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(22316..24889)
                     /gene="mprF"
                     /locus_tag="ACHA52_000022"
     CDS             complement(22316..24889)
                     /gene="mprF"
                     /locus_tag="ACHA52_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8938787.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            25030..25836
                     /gene="proC"
                     /locus_tag="ACHA52_000023"
     CDS             25030..25836
                     /gene="proC"
                     /locus_tag="ACHA52_000023"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8938788.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(25915..27471)
                     /gene="rny"
                     /locus_tag="ACHA52_000024"
     CDS             complement(25915..27471)
                     /gene="rny"
                     /locus_tag="ACHA52_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8938789.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(27882..28931)
                     /gene="recA"
                     /locus_tag="ACHA52_000025"
     CDS             complement(27882..28931)
                     /gene="recA"
                     /locus_tag="ACHA52_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8938790.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(29040..30281)
                     /locus_tag="ACHA52_000026"
     CDS             complement(29040..30281)
                     /locus_tag="ACHA52_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8938791.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(30521..31099)
                     /gene="pgsA"
                     /locus_tag="ACHA52_000027"
     CDS             complement(30521..31099)
                     /gene="pgsA"
                     /locus_tag="ACHA52_000027"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8938792.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(31308..32231)
                     /locus_tag="ACHA52_000028"
     CDS             complement(31308..32231)
                     /locus_tag="ACHA52_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8938793.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(32970..34256)
                     /locus_tag="ACHA52_000029"
     CDS             complement(32970..34256)
                     /locus_tag="ACHA52_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8938794.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(34249..35517)
                     /locus_tag="ACHA52_000030"
     CDS             complement(34249..35517)
                     /locus_tag="ACHA52_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8938795.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            35769..36230
                     /gene="mscL"
                     /locus_tag="ACHA52_000031"
     CDS             35769..36230
                     /gene="mscL"
                     /locus_tag="ACHA52_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8938796.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(36289..36735)
                     /locus_tag="ACHA52_000032"
     CDS             complement(36289..36735)
                     /locus_tag="ACHA52_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8938797.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(36828..37073)
                     /locus_tag="ACHA52_000033"
     CDS             complement(36828..37073)
                     /locus_tag="ACHA52_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8938798.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(37188..37766)
                     /locus_tag="ACHA52_000034"
     CDS             complement(37188..37766)
                     /locus_tag="ACHA52_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8938799.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(37843..38373)
                     /locus_tag="ACHA52_000035"
     CDS             complement(37843..38373)
                     /locus_tag="ACHA52_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8938800.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(38377..39237)
                     /gene="rlmB"
                     /locus_tag="ACHA52_000036"
     CDS             complement(38377..39237)
                     /gene="rlmB"
                     /locus_tag="ACHA52_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8938801.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(39230..39625)
                     /locus_tag="ACHA52_000037"
     CDS             complement(39230..39625)
                     /locus_tag="ACHA52_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8938802.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(39622..41034)
                     /gene="cysS"
                     /locus_tag="ACHA52_000038"
     CDS             complement(39622..41034)
                     /gene="cysS"
                     /locus_tag="ACHA52_000038"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8938803.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(41031..41567)
                     /gene="epsC"
                     /locus_tag="ACHA52_000039"
     CDS             complement(41031..41567)
                     /gene="epsC"
                     /locus_tag="ACHA52_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8938804.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(41653..41892)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41889..43349)
                     /gene="gltX"
                     /locus_tag="ACHA52_000040"
     CDS             complement(41889..43349)
                     /gene="gltX"
                     /locus_tag="ACHA52_000040"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8938805.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(43519..44676)
                     /locus_tag="ACHA52_000041"
     CDS             complement(43519..44676)
                     /locus_tag="ACHA52_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8938806.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(44710..46080)
                     /gene="radA"
                     /locus_tag="ACHA52_000042"
     CDS             complement(44710..46080)
                     /gene="radA"
                     /locus_tag="ACHA52_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8938807.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(46143..46670)
                     /locus_tag="ACHA52_000043"
     CDS             complement(46143..46670)
                     /locus_tag="ACHA52_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8938808.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            46900..47706
                     /gene="proB"
                     /locus_tag="ACHA52_000044"
     CDS             46900..47706
                     /gene="proB"
                     /locus_tag="ACHA52_000044"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8938809.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            47703..48950
                     /locus_tag="ACHA52_000045"
     CDS             47703..48950
                     /locus_tag="ACHA52_000045"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8938810.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            49026..49370
                     /locus_tag="ACHA52_000046"
     CDS             49026..49370
                     /locus_tag="ACHA52_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938811.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(49487..49813)
                     /locus_tag="ACHA52_000047"
     CDS             complement(49487..49813)
                     /locus_tag="ACHA52_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938812.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(50428..51630)
                     /locus_tag="ACHA52_000048"
     CDS             complement(50428..51630)
                     /locus_tag="ACHA52_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8938813.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            51747..52262
                     /locus_tag="ACHA52_000049"
     CDS             51747..52262
                     /locus_tag="ACHA52_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8938814.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(52461..52907)
                     /locus_tag="ACHA52_000050"
     CDS             complement(52461..52907)
                     /locus_tag="ACHA52_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8938815.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(52954..54432)
                     /gene="msr(C)"
                     /locus_tag="ACHA52_000051"
     CDS             complement(52954..54432)
                     /gene="msr(C)"
                     /locus_tag="ACHA52_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8938816.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(54786..55415)
                     /locus_tag="ACHA52_000052"
     CDS             complement(54786..55415)
                     /locus_tag="ACHA52_000052"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8938817.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(55399..55863)
                     /locus_tag="ACHA52_000053"
     CDS             complement(55399..55863)
                     /locus_tag="ACHA52_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8938818.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(56350..57618)
                     /locus_tag="ACHA52_000054"
     CDS             complement(56350..57618)
                     /locus_tag="ACHA52_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8938819.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(57849..58586)
                     /locus_tag="ACHA52_000055"
     CDS             complement(57849..58586)
                     /locus_tag="ACHA52_000055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8938820.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(58653..59222)
                     /locus_tag="ACHA52_000056"
     CDS             complement(58653..59222)
                     /locus_tag="ACHA52_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8938821.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            59350..60222
                     /locus_tag="ACHA52_000057"
     CDS             59350..60222
                     /locus_tag="ACHA52_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8938822.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            60564..61436
                     /locus_tag="ACHA52_000058"
     CDS             60564..61436
                     /locus_tag="ACHA52_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8938823.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            61433..63391
                     /gene="nagE"
                     /locus_tag="ACHA52_000059"
     CDS             61433..63391
                     /gene="nagE"
                     /locus_tag="ACHA52_000059"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8938824.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(63723..64574)
                     /locus_tag="ACHA52_000060"
     CDS             complement(63723..64574)
                     /locus_tag="ACHA52_000060"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8938825.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            64697..65164
                     /locus_tag="ACHA52_000061"
     CDS             64697..65164
                     /locus_tag="ACHA52_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8938826.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(65427..65909)
                     /locus_tag="ACHA52_000062"
     CDS             complement(65427..65909)
                     /locus_tag="ACHA52_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8938827.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(66009..66872)
                     /locus_tag="ACHA52_000063"
     CDS             complement(66009..66872)
                     /locus_tag="ACHA52_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8938828.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            67470..68345
                     /locus_tag="ACHA52_000064"
     CDS             67470..68345
                     /locus_tag="ACHA52_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8938829.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(68490..68642)
                     /locus_tag="ACHA52_000065"
                     /pseudo
     CDS             complement(68490..68642)
                     /locus_tag="ACHA52_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(68910..70241)
                     /locus_tag="ACHA52_000066"
     CDS             complement(68910..70241)
                     /locus_tag="ACHA52_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8938830.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(70390..71103)
                     /locus_tag="ACHA52_000067"
     CDS             complement(70390..71103)
                     /locus_tag="ACHA52_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8938831.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            71278..72702
                     /locus_tag="ACHA52_000068"
     CDS             71278..72702
                     /locus_tag="ACHA52_000068"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8938832.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            73001..73708
                     /locus_tag="ACHA52_000069"
     CDS             73001..73708
                     /locus_tag="ACHA52_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8938833.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            73695..74018
                     /locus_tag="ACHA52_000070"
     CDS             73695..74018
                     /locus_tag="ACHA52_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8938834.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(74107..75144)
                     /locus_tag="ACHA52_000071"
     CDS             complement(74107..75144)
                     /locus_tag="ACHA52_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8938835.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(75465..76448)
                     /locus_tag="ACHA52_000072"
     CDS             complement(75465..76448)
                     /locus_tag="ACHA52_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8938836.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(76863..77633)
                     /locus_tag="ACHA52_000073"
     CDS             complement(76863..77633)
                     /locus_tag="ACHA52_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8938837.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(77626..78561)
                     /locus_tag="ACHA52_000074"
     CDS             complement(77626..78561)
                     /locus_tag="ACHA52_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8938838.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(78877..80526)
                     /locus_tag="ACHA52_000075"
     CDS             complement(78877..80526)
                     /locus_tag="ACHA52_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8938839.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(80643..83246)
                     /locus_tag="ACHA52_000076"
     CDS             complement(80643..83246)
                     /locus_tag="ACHA52_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8938840.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(83258..84307)
                     /locus_tag="ACHA52_000077"
     CDS             complement(83258..84307)
                     /locus_tag="ACHA52_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8938841.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(84333..84647)
                     /locus_tag="ACHA52_000078"
     CDS             complement(84333..84647)
                     /locus_tag="ACHA52_000078"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8938842.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(84663..85109)
                     /locus_tag="ACHA52_000079"
     CDS             complement(84663..85109)
                     /locus_tag="ACHA52_000079"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8938843.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(85110..87032)
                     /locus_tag="ACHA52_000080"
     CDS             complement(85110..87032)
                     /locus_tag="ACHA52_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8938844.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(87244..88140)
                     /locus_tag="ACHA52_000081"
     CDS             complement(87244..88140)
                     /locus_tag="ACHA52_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8938845.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(88133..88813)
                     /locus_tag="ACHA52_000082"
     CDS             complement(88133..88813)
                     /locus_tag="ACHA52_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8938846.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(88814..89596)
                     /locus_tag="ACHA52_000083"
     CDS             complement(88814..89596)
                     /locus_tag="ACHA52_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8938847.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(89589..90506)
                     /locus_tag="ACHA52_000084"
     CDS             complement(89589..90506)
                     /locus_tag="ACHA52_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8938848.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(90670..90924)
                     /locus_tag="ACHA52_000085"
     CDS             complement(90670..90924)
                     /locus_tag="ACHA52_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938849.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            91781..95008
                     /gene="fss3"
                     /locus_tag="ACHA52_000086"
     CDS             91781..95008
                     /gene="fss3"
                     /locus_tag="ACHA52_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8938850.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADETTHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            95121..95435
                     /locus_tag="ACHA52_000087"
     CDS             95121..95435
                     /locus_tag="ACHA52_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8938851.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            95448..95822
                     /locus_tag="ACHA52_000088"
     CDS             95448..95822
                     /locus_tag="ACHA52_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8938852.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            95832..96167
                     /locus_tag="ACHA52_000089"
     CDS             95832..96167
                     /locus_tag="ACHA52_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8938853.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            96250..97590
                     /locus_tag="ACHA52_000090"
     CDS             96250..97590
                     /locus_tag="ACHA52_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8938854.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            97668..98342
                     /locus_tag="ACHA52_000091"
     CDS             97668..98342
                     /locus_tag="ACHA52_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8938855.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            98335..98499
                     /locus_tag="ACHA52_000092"
     CDS             98335..98499
                     /locus_tag="ACHA52_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938856.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            98575..99717
                     /gene="dcm"
                     /locus_tag="ACHA52_000093"
                     /pseudo
     CDS             98575..99717
                     /gene="dcm"
                     /locus_tag="ACHA52_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            99707..99997
                     /locus_tag="ACHA52_000094"
     CDS             99707..99997
                     /locus_tag="ACHA52_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938857.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            100256..101440
                     /gene="mobT"
                     /locus_tag="ACHA52_000095"
     CDS             100256..101440
                     /gene="mobT"
                     /locus_tag="ACHA52_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8938858.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            101437..101574
                     /locus_tag="ACHA52_000096"
     CDS             101437..101574
                     /locus_tag="ACHA52_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8938859.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            102320..104230
                     /gene="ltrA"
                     /locus_tag="ACHA52_000097"
     CDS             102320..104230
                     /gene="ltrA"
                     /locus_tag="ACHA52_000097"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8938860.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            104334..104558
                     /locus_tag="ACHA52_000098"
     CDS             104334..104558
                     /locus_tag="ACHA52_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938861.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            104571..105074
                     /locus_tag="ACHA52_000099"
     CDS             104571..105074
                     /locus_tag="ACHA52_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8938862.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            105134..105523
                     /locus_tag="ACHA52_000100"
     CDS             105134..105523
                     /locus_tag="ACHA52_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8938863.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            105510..107957
                     /locus_tag="ACHA52_000101"
     CDS             105510..107957
                     /locus_tag="ACHA52_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8938864.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            107962..110085
                     /locus_tag="ACHA52_000102"
     CDS             107962..110085
                     /locus_tag="ACHA52_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8938865.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            110082..111086
                     /locus_tag="ACHA52_000103"
     CDS             110082..111086
                     /locus_tag="ACHA52_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8938866.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            111105..112016
                     /locus_tag="ACHA52_000104"
     CDS             111105..112016
                     /locus_tag="ACHA52_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8938867.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            112203..112459
                     /locus_tag="ACHA52_000105"
                     /pseudo
     CDS             112203..112459
                     /locus_tag="ACHA52_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(112676..112891)
                     /locus_tag="ACHA52_000106"
     CDS             complement(112676..112891)
                     /locus_tag="ACHA52_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938868.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(113140..113358)
                     /locus_tag="ACHA52_000107"
                     /pseudo
     CDS             complement(113140..113358)
                     /locus_tag="ACHA52_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(113653..115092)
                     /locus_tag="ACHA52_000108"
     CDS             complement(113653..115092)
                     /locus_tag="ACHA52_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8938869.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            complement(115680..116312)
                     /locus_tag="ACHA52_000109"
     CDS             complement(115680..116312)
                     /locus_tag="ACHA52_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8938870.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            complement(116661..118112)
                     /locus_tag="ACHA52_000110"
     CDS             complement(116661..118112)
                     /locus_tag="ACHA52_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8938871.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(118224..119567)
                     /locus_tag="ACHA52_000111"
     CDS             complement(118224..119567)
                     /locus_tag="ACHA52_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8938872.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(119701..>121108)
                     /locus_tag="ACHA52_000112"
     CDS             complement(119701..>121108)
                     /locus_tag="ACHA52_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8938873.1"
                     /translation="TVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNT
                     PGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVT
                     NLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNAD
                     KYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYP
                     DGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTW
                     KEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGK
                     TEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVV
                     VSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLI
                     LLGTLGLLGYKNRKKENE"
BASE COUNT        35804 a        24581 c        21945 g        38778 t
ORIGIN      
        1 agaatgcaac aaatcaatgt cgcattccct tctgatatat tttattcagc acttattttg
       61 aactttctga ggtgctagct gatgaacttg tggagctgga agattcttct tttgaagagt
      121 tgcttgtgct actggagcta cttgagctgc tagaactgtc tgaagagtct ttttcagaac
      181 tagaagacgc tggatctggt cctttagagt aaattacgtt cacaaccatt cctttggtaa
      241 ttgtcgttcc tgcccctggt actgtacgaa tcactttatc tttatccacc gtgtttgaat
      301 attcggaagt ctcatgcccg atatattctg caccgacact agctaaataa ctttgtgctt
      361 cgctttttgt atagccggag atatcattca atttaggctc tgttccttgg cttacgacaa
      421 aattgatgta agtttcttct gctacaactt cacccgaggc tggatcctga ctgatgatcg
      481 aacctgatgg tacagtgtcg ctatagtcat aagtagcact aatattattg atattgaatc
      541 cttgtgtaac aagccaatta cgaacagttt cgtagttttc tcctacatga ctactcaaag
      601 tcactgtttc tggtcctttg ctgactgtga aagtaatcgt gtctgtttta ggatcgactt
      661 ctgtatcttc tgatggagat tgcgaaatga tcattccgct ttctacctct gaactgaatt
      721 cctcttcttt ttgaatcaag tcagcagaaa accctaattc tttcaaactt gcttcggcat
      781 cttcataact tttaccactg taatcatcta atttgatttt tttcgtgccg ctactaatat
      841 acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct gtcttcacta
      901 cattaccttc ttcgattttg tcatctgcaa tttcttctgt ttttgttgct acttctaatt
      961 tcgcttttgc gagtgtttct ctagcagaag cttcagaaag atcagaaaca tcaggaacaa
     1021 caacttctcc tcgcccgcca gaagcaaaga atacagctgt tccgattcct aaaacagcaa
     1081 gaatagctaa taggatcagc caccattttt tctgtccttt ctttttagga tcaggcagaa
     1141 cttcttcctc ctctggtaag gtttcgtttt ctggcttgtc ttttggcaga ggcatagatt
     1201 tgaatgaatc aggcattggt gcatcttccg taaggggtgt gataactttt gtttcattat
     1261 ccatcacatg aggctgccat ttttcttcgt ttgcacgagc tggtgataat actgtagaaa
     1321 gatcatcaga catttcctcg gctgacttat aacggtctgc aggttcttta gccgttgccc
     1381 gtaaaatcac gttttccagt gcttgtggca catttggatc aagtgctttt atagatggaa
     1441 gatcatcttg aaaatgtttt aaagcaattg ttacagcaga ttcaccatca aacggtacgc
     1501 tacccgtcaa catctcataa aggatgatcc ctaaagcata aatatctgac tgcttcgttg
     1561 ccatacttcc tctggcctgc tcaggggaca gataatgaac agatcctagc atcgtattcg
     1621 tctgcgtgat cgatgtttca gataaagcga tagcaatccc gaaatccgtg atttttacaa
     1681 caccttcatt atcgatcagt acgttttggg gtttcaaatc tcggtggatg atttgatggt
     1741 tatgagctaa agaaatagca gaaaggatct gctgcataat atttactgct gtttcataag
     1801 gaacaggata atgtgtttgg atatatcgtt tcaagtccat tcctttgacg tattccatca
     1861 ccagatactg catgttgtct tcttcaccaa catcgtaaac actaacaatg tttggatgaa
     1921 cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc tggtcatttt
     1981 gaaagtcaaa tcgtaagact ttaactgcta catcacgatc caagattaaa tcgtgcgcca
     2041 aaaatacatt tgccatacca ccgctaccga tatttccagt gatttgataa cggccattta
     2101 attttttgcc caactcgatc atacactgtc ctccttatat tcgataagaa gaacggtgat
     2161 attatccgct ccgcctgctt cattggcacg atcaatcagc tcagttactt tatgagacaa
     2221 tgggccttct tgattgatga tcgttcctat ttcttcttca gacagcatgt tcgtcaagcc
     2281 atctgaacat aacagcagtc gatcttttag ttgccaaaga tgagtagaaa catctacttc
     2341 gactgttcca ggcatgccga cagaacgtgt taaaatattt ttccttggat ggttcactgc
     2401 catttcttcg ctgatttctc ctgatttgac tagttcattg actaaagaat gatcctccgt
     2461 caattgcatg attttatctt cacgaatcaa ataagctctg ctatccccaa cattggcaat
     2521 cgtgaaccag tcatctgaca gagcggccgc tacgattgtt gttcccatac cgttatattc
     2581 cggctgttct tgtccacgct gataaatcag catattttct tcttgaatcg attgaatgaa
     2641 ccattgggcg attttttcat cgtcaacagc ttcttgtttt tcccaagctt cgcctagatt
     2701 cgttaccgcc atctgactgg caatgtctcc ggcacgatga ccacccatcc cgtctgctaa
     2761 tacagcaagc ttgagacctg aacggctggt aaatacactg gcatagtctt gatttgtgtt
     2821 acgtcgtctt cctacatctg attgatattc aatctgcatt ttttcacctc attacttttt
     2881 gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga tgcggataca aagtcaacat
     2941 tttctcttcg attgatgatt gtagcatagg attggtcaaa acatcaatcc tctcaaaatc
     3001 agggtgtctt tccaagaaag cagaaacaac gtcttggttt tcttctttta agatcgtaca
     3061 agtgctataa gtcagaatac cggaagactt taaagttggt gcgcagcttt ctaaaatttc
     3121 caattggatt tttggtaaat tcgctaattc atccggctgt tttttataac gaatatctgg
     3181 ttttcgccga agcagcccaa gacctgaaca aggcgcgtct actaaaatcc gatcaaactg
     3241 ctctgcttcg aactgtttac tgacttctct agcatccatc ttttgagcgt agacagcaga
     3301 agagacacct agacgtgctg cattatcttc aatgagtttg actttgtgtt cgtgtacatc
     3361 taaagcgacc acttctccac cgcatgacgg atctaaaaaa gaagcaatat gcgtcgtctt
     3421 acctccgggt gctgcacagg catctaagac atgatgttca ggctcgattt gcatagacgg
     3481 cgctacaagc atcgaacttt catcttggat cgtcatgacg ccatcgtgga acaatgagct
     3541 agaagctaaa tgcccttttt tcgctactat accgtatgga ctgacttggc ttttttcagc
     3601 tgtgatctct tcttcttgca aaacagtgat ggcctgatct ctggaaagtc ggcgtgtatc
     3661 tactcgggca ctagcatggc taggctgata tagcgataaa ccgagctttt cagtttcttc
     3721 gtatccgatt tgatccacca gtcgtttagt catccataga ggcaggctga ttttgacaga
     3781 aagtctttcg ataggatcag caatgccttg cagatctggt gctccttgac gctgataatt
     3841 cctcaagaca ccatttacaa atttccctgt cccaggattt ccttttactt ttgcgatttc
     3901 gaccgcctcg tttaaaatag catgtgcagg gactttatct aaaaattcta attggtatac
     3961 agaaagataa agaagcgtct tgacccagtc atccactttt tttgctttag cgatcaatgg
     4021 tgcaacgtaa aattccaata gtcgctgatg gctaatcgta ccatagatca attctgtaaa
     4081 taaacccaca tctttttgat ttagctcgct ttgtttgatc atctcattca acaaaagatt
     4141 cgagtaggcg ccacccttgt tcactcgctc taatgtcagt aatgccatat atcgtactga
     4201 ttttttcatt tttttaggga tttttttcat tatttcacct gctgaccaat ctgaacttgc
     4261 tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc ctttaccagc tggctgtaat
     4321 tcattgatgg ctaagacggt ctgtttaccg caagcaatcc atagattttt tttgtctttc
     4381 cggatgatcg taccaggtgc tttgtctgta gtttctgcca atacttcaac cgataataat
     4441 ttccagcgta tgccctcata ggtcgtgaat gcaattggcc aaggacgcat tccgcgtact
     4501 tggttgtcaa tttcttctgc agttttatgc caatcgattg cttcttgttc ccgtgtgatg
     4561 tttggcgaaa aagttgcttc tgactcgtct tgaggtactg gtttttgtcc atccaaaata
     4621 ttgggcaatg tttctagcaa tagctttctt cctaaaaggc taagcttttc aaacatcgtc
     4681 cctacatcat cttgtttagt gattggtatg ctttcttgcg catagattcc gcctgcatcc
     4741 atttttttga tcatttccat gatcgttaca ccagtttctt tctccccatt catgatggca
     4801 taatgaacag gcgcacctcc ccgatattta ggaagtaaag aagcatggac attgattgcc
     4861 cctaattttg gtacttgcaa taacttttca ggcaagaatt ggccaaatgc cgcagtgacg
     4921 atcacatcag gcaccaattc aatgatttct tccatttctg gcgaaccgga gattttctct
     4981 ggctgcagca cacggatacc gtgcttcaga gccgcttctt ttaccggtgt cggcgtaatt
     5041 actcgtttcc gcccaactgg acggtcgggc tgtgttacca ctgcctggat ctcgtaacct
     5101 gattctatca gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat aaatactagc
     5161 ttagtcatca agatgttcct ccatatattc ttcaagttct tcttccggga tctgctgtat
     5221 cattttctca atgaacaaga taccgtctaa gtgatcgatt tcatgctgga aggcacgggc
     5281 taaatagcca aaagccgtca cttctatctc ttctccgtct cgatcataat aacgcacggt
     5341 cacttcatct gctcgcttga ccgttccata gacatgcgga atactgagac aaccttctac
     5401 gtcaaggctt tcaccttttg cttcgatgat ttctgggtta atcagttcaa atttctcgcc
     5461 ttcatctact tcgatcacag caattctctt gttttgtcct acttgaggag cagcgatccc
     5521 tattccgtca ttcgcaatca ttgtctcata taaattgtct aataaagcga ttgtctcgtc
     5581 cgtgattaca tcgattggct gagcagttct ttttaattta tcattagggt ggatcaatat
     5641 aggatagcgc ataatttggc cctttcttaa ataaagttta atggttcgtt gtcaattgcc
     5701 acaaaaagac cttgtcgctg ttctttctgg cttccattca aaatttcatc caaaacatga
     5761 ggaagattcg gttcatgttt atatttgatg attagttgat aataatatcg gtttttcact
     5821 ctcaaaatca tgctgggcgt tggccctaat agcaagcttt gagggcttaa cacttctttt
     5881 agctgattgg caatctgaaa gattttcttt gccgctactt gttcttcagg atggctggcg
     5941 gttattttca cagtaaagta ataaggagga tagcctccac gatgccgtac atgcatttcc
     6001 tgctgataaa actgctcata attttgtttt ttagccaatt ctatggcgta atgttgagga
     6061 ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc gccctgctcg tcctgaaact
     6121 tgggtaagca attggaatgt atgctcacta gaacgaaaat ccggtaaatt cagggaggta
     6181 tcagcattca acaccccaac taatgtcact tctggaaagt ccaaaccttt agcaatcatt
     6241 tgtgtcccta ataatatatc tgcttctttt gcaccgaatt tttgtaaaat ctgttcgtgt
     6301 gccccttttc ttcgagtggt atctacatcc atacgcaaaa tcctagcttg cggataaagt
     6361 tctcggagtt cctcttctac tttttgagta cctgttccat agtagcggat tttattgccg
     6421 ccacaattag gacagcgatt gggaataggt tcttcatgtc cgcaataatg gcagcgcatt
     6481 gaacgtgtat ccatatgcag tgtaagagag atatcgcaat taggacatgg aagaacaaat
     6541 ccacagtccc tacacatcac aaaagacgaa tatcctcgac gattgagcag taggacagtt
     6601 tgttcttttt tgtctaatcg attttgtatt ttttcctgca aattagcaga aaaagtagag
     6661 gtccgatgat tctgaaactc ttcacgcata tctacgatct caatagctgg aagctgtgca
     6721 gctgctttgg ctcggtggtt taactgcaat aactgataaa cgcctttttg agctcgtgct
     6781 cttgattcta aagagggcgt cgcgcttcct agaactattg ggcaatgatg atatttactg
     6841 cgccaaattg ctagatctct cgcatgataa cgcggtgtct catcttgttt gtaacttgat
     6901 tcatgttctt catcgataat gatcagcccg atatttttca gcggggcaaa aatagcagat
     6961 cgtgcgccta ctactacttg cgcttcttcc cgctcaatct tgcgccattc atcatatttt
     7021 tcaccttggg aaagtccgct atgtaagacc gcaactgatt ctccgaagcg tcctttgaat
     7081 cgctcgacca tttgcggagt caatgcgatt tcaggcacta acataatagc cgttttattt
     7141 tctgacaaaa catcagcgat tgcctgaaga tatacttctg ttttcccgct gccagtgatt
     7201 ccctccagca aaaagacctg atctttttgc tgctgacctg ctgtgatgat ttgttcgaca
     7261 gcattttttt gttctgcatt cagttccaat gccgtcgttt ggtcaaatac tcggtcttta
     7321 taagggtcgc gataacgttc tgattctata aactccagcc agcccttttc ttttccttga
     7381 ttcaagacag cagtactgat tcctttattt ttgaaataag caattggttc ttcttggctc
     7441 agttcagata aacacaaaag aagttctttt tgtttcttgg cattttgccg taattttgcc
     7501 cattcttctt cgatttgctt ttcagttaac aaagaacgga ccaaacgaat cgtttttact
     7561 ttatttcgtg tatggacttc atatttgata tctactaatt gctcttggcg ccagcgaatc
     7621 aattgagaaa ggctgccttc ttcttctgct tccttccagg aaatcacttc tcgctgatca
     7681 aacaatacct gaacttctgg tgtgtctgcc aaaggataga tcaatttatc atattctgct
     7741 ttcatcacac tcggaagcat cgtttgtaga caagtgattt tgaatgcgta agtaattttt
     7801 ttcatataat cagctaatga aagtaattct tcattgacca ccggagcaag gtctagcaaa
     7861 cgaataactg ccttcaaaga tggattagct gattcttctg atttttggat agccataaca
     7921 aatccttgga catgacgatt gccattacca aaagggactt ctactcgcat cccgacttgc
     7981 aaagctgttt ccatttcaga gggaaccaaa taagtgaatg gctgatcggt ctgcatagta
     8041 ggtacgtcca caatgacttc agctgttaat gtcatggttc gtttcctcct ttttttgtct
     8101 tttcttatca agcgacaaaa agttccttgc ctattttact agagatcgat ggatttgtct
     8161 tatcttctgc caaatcctaa caaaaaatta acagcagata agaaaaaaaa gaactgtgtc
     8221 aaaaagttca gacacagcct tttctaccag acaataagtg gcgaatgttt gaaaaggaaa
     8281 cagctgggtt tgcttttctt tttagatgtt cgctctattt ttctttatta cattttttgc
     8341 tcttcacgga tacgtgattc caattcgtgt tgttcacgct ctttttgtgc ttgacgttcc
     8401 tcttcttcca tcttcaaacg ttcacgtttt aattcaggat gtggatcatt gactacattt
     8461 cctgcatcga tttcttctaa agcttgaccg acgcttttta ctgaatcaaa agaatctaga
     8521 gtaggctgtg cacttgcatc caattcatgc gcgcgtttac ttgctaaaat cactagagaa
     8581 tattttgaat ttacacgatc taatagtgag tcaattgaag gttttaacat cataagacta
     8641 catctccttt aacattttaa tatatttacc gattacgcga tctactcgaa aatgttcact
     8701 ggcaataatg tttttgattc gttcaactgc tagcggcact tcatcattta caacagcata
     8761 gtcgtaaaga gccatcattt cgatttcttc ttttgcaact ttcattcgtt cttcgattac
     8821 ttcatctgca tctgttcctc ggcctacgat ccgtgatttt aattccgcta aatctggcgg
     8881 agtcaaaaag atgaatacac catcgggcac tttttctttg acttgttttg ctccttggac
     8941 ttcaatctcc aagaaaacat cttttccttc atccaatgtc ttgttgacat atgacaaagg
     9001 agttccataa taattcccga catattctgc gtattccagc atctctccag cttcgatcat
     9061 tgattcaaac tcttctttgc ttcggaagaa ataatcaacg ccttcgacct ccccttcacg
     9121 cattttacgg gtagtcattg agatcgaata ctgaaaatca ttatcctcac tctcaaaaat
     9181 tgcttttcgt actgtgcctt ttcctacacc ggatggacca gacaacacga ttaataatcc
     9241 acgctctgac atgacgacgt cctttcaact agtctttttg tacttatcgt ttattttgca
     9301 ctttttttct gcaactttca aggttatctt taattatagc tagaaattgg taaaaaagct
     9361 accctgtacc aagaggtcgt aaaaaagctg tcctgcatca agtaataaga acagccaaac
     9421 ataaacagct tctcatgttt ttcgtttggt tggggttatt atcgcagatg caagcttttg
     9481 aacgccgtgt ataagaggtc gtgaagaagc cgattagttt cgagtatgaa gaaaaatttt
     9541 gtgaaaatag tttctcacat ttttgcataa atttggctta ataccgagaa aatggttttt
     9601 gaacatcact tattcaaaat aaaaaaagat tgtgacacga attttgtcac aacctttaaa
     9661 tgtattccta gagcaaagaa gctaagcgaa gaaatccaat tctgattatt tattgtattt
     9721 ctttgttcag catccttgct aaaagcccat attggacaaa gtattcacta cacccatgat
     9781 ctcatctttt cgagaacgtg ctttttctgt cggatcttct acctcttctg tgtcaattcg
     9841 aacaataaat ttccgattca tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc
     9901 gttctcatta tagtctgcac aggcatcttt ccatacgtca aagccgtttt tttcagaagg
     9961 gatatcccgt ttaaccacag tttttccact taattcgtgg aacgttacgt cataatattt
    10021 cattatctct acctctttcg tcattcaata ttttgtactt gttctctgat tttttctaag
    10081 atcgtcttag actggacgac ttgattttta atttcgattg catttgattt ggaaccgatc
    10141 gtgttgattt cccgattcat ttcttggatc aggaaatcta attcccgacc tacaggacat
    10201 tgtgcttcga taagttcatc tagtttttgt acatggatcg ccagacgatc cagttcctcg
    10261 tgaatatctc cgcgttccaa caagatggct agctccgtca acaaccgttg ctcatcgact
    10321 ttcgctccaa gaaattcctc cagctttttt tgatacctta tttgaaattc cttttcatag
    10381 atttctacaa aactatcgag tttttttagt acttctttga attcttcagt attctttttc
    10441 aacactaaag ctaaagcttg accttctttt tctcgactcg cctcaataga atcaagtgcg
    10501 cttttcaatg tatctagagc taactgtgcc aattcttcca tgctctcttc cggtttttct
    10561 tctaccgtta cgaagttttc gtccagcgcg aagcgttccg ccatccgact aattgacact
    10621 tgattttgac taccgtatcg ttgttgaatt ccgtctgtca attgagtgat aagtgaatca
    10681 atcaattccc agttgatgac gatctgtttt tcgtttttgc caatataagc tagattcaca
    10741 aatacttcga ttcgaccgcg gcttaactgc tgtttaataa gctgacgaaa atcgttttca
    10801 acaaagttca ggattttagg acttcgtatt tgaacatcta gaaaacgctg gttgacactt
    10861 tttatttcaa tatctaactg atacgctttt gtttcgcgga tggctttgcc aaaaccagtc
    10921 atacttttca ttcgacaggg tccttcctca ttacatattt tctattgctt tttgcagttg
    10981 ttcaaactct tcgttcgtca atgtcaggcc tttccccatt ttttcatggt ctggcgccca
    11041 atctcgaaga tcgaattttg gcgggcgacc attccagctg actaggttca gttctttacg
    11101 ccaccccttg ttgttttctg acaatatagc gatttcttct acgatttcat atgaaaattc
    11161 ttgtgccatg gttgttcctc cttgagataa tataaatata ggatcagtaa tgtgtcaact
    11221 ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt ttccttgtag cagataatca
    11281 aaataaacag ttttcgtgcg ctcattttgt attttttgct ggtattttcg ataccttcgc
    11341 cattcttcag tacttaattg accaatttgt gccaataaat catccggcat actttttata
    11401 tcttgttggc agatcatttg aatcatcatg tctacttgat agatttcatt gggcagtaaa
    11461 aaatgttttt ttgctaaaag ttctagcatc gccaatccgc atgtctctgg tacgaccctg
    11521 ttcttatata aattacgcag tttttgccaa aattttatgc ttttgaaaaa agataattct
    11581 aactgaattt cttttgttaa gtaagacaaa aatcctcgcc agtatgttct tgcaggttta
    11641 acagaatgaa atgtatacca gttcccatag taatagccta atcgtttttt catttccaga
    11701 tagcctaatt gtaaatttaa ttggttcctt tggctatcaa aaagcagaaa gctgcctagt
    11761 gtccacaatg tttgacattt ccaatggata aaaatgtctg gcatacgaat ttttttcgct
    11821 gggcctggcc cttgcacatc tacgacaaat gcctctgttg ctcctttgtt aatcgcaata
    11881 tcaataggta ttttgttgcg atacccaccg tctgcatatt tattttttcc gatctttcga
    11941 taagccatag cgggataaaa tgaagcagag gcgagtatcc aatctggtat ttcttctgtt
    12001 tttgtctgtt ggatatcagt cactacttcc gctagttctg gcagtttagt agacaccgta
    12061 tataaacgta ttttgggatt atgtttgagt aaggacaaat ccaaattgtc tttgaccatc
    12121 ttttccaatg gagcagaaga aaccccgcct tcaattagtg ctgttttcgt catttggcgt
    12181 ctttctcgat agaagcgttc acgaaggtct tcaactgcag ccatttctgg taacgcaagc
    12241 acttgtcttg tcgacagctt tttccataaa ttcacagctt ttttcatatc tccttgtaaa
    12301 atcaatgcgc cgttcaacgc acctaccgag gtacctgtga taatctcaaa gttgatatca
    12361 tgttccttca gcgcctgcca cacaccaatc tgataagcac cgtgtgctcc tcctccacct
    12421 aaaacaagtg ctgtatgata gaccagcttt ttatttaatt gtgtggttcc ttgatagccg
    12481 tttttatgaa aaatacgcaa ccattcttct gatagaggag attgaaataa aaaacaacag
    12541 ttttctttaa taaaaaaacg agcgcaagct tccatatacc gaaaaaattt tttccattca
    12601 aaagtttcac cttcaatcaa cagattatta aaaataacat ctcctctact tttagcaata
    12661 gtaaaataga cggtatattt tttcgatact gctttaaaag cacattgctt ttttgcagaa
    12721 cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa atggaagggg ttgttttgct
    12781 tgctggatag gcgaatacag cgacattttc tgtgaaaagt aaactttttt tatctccatc
    12841 gcgctctcct ttttcttcaa gtataccagt acgtcataaa aaggtcatga gtctcgtatt
    12901 cgactttgta gattccataa gaaaacaatt cataaaacat cctttaaata aaaaatacaa
    12961 aaatcacaaa aattaattga aaatgagagt gttttcaatg aaaacgtgat tttgtaatgg
    13021 aattttaaaa taaaagaaac aagctttttt aatgcaaatg ttgtaaaata aaagagatta
    13081 agaggaaata agaggaggaa catgatgact gagtcagttt atgtacatat tgataccaca
    13141 agtaatgctg ttttaacaaa agggctgact gctactgact ttgcaaacag tatcgtgcat
    13201 tttcctcaaa acttgctctt gcttgatcca tctgcttctg ctggagaata tgaatcccat
    13261 acagggctga aagtaatccg tggtacagaa aatattcagc gtttcttttc ctcttctcgt
    13321 aacaggcgtg gattcgatga attgaagtgg atcgacttta ctgatttaac gatgctgaaa
    13381 gaattgacac ctttagaaat ttcagaatta ttatattttg gtcatatgaa aacacatctt
    13441 cattcgccgt ttttttataa actacagaat aattttgtat acttcgatct taatgatcag
    13501 ctgaatcgga tttattatcg ctatttagat gaattttata ggatttttgc tagtaaatta
    13561 agttcgatct tgtcagataa gatcaatgta aaaaaatctt ttttcaaaaa agaaacacct
    13621 gttgaaaaaa tcagtgtgga attattgaaa gaaatgcgta atttgatgca ggaagggatc
    13681 attttttctt ttgatcaagt tggtttgatc gatggtaaat atcgtatccc aatctatgtg
    13741 attgaagatt atatttggaa attaaaaggc acccggtatc gagaagagga aatcatcgct
    13801 actttaatct actctgtcac cgatcgacac tggcaattgg tagaagatgc cgatctttct
    13861 tatctgccat tgaagtagat ctatttctta tcaaaaaata aaaaggaaac aagaaactag
    13921 gacatgattc tgtctcaagt ttcttgcttc ttttttattt acagctcctg tcgtttatct
    13981 ggcacaaaaa tcgaaggagt cgtttgttgg atccctaata aaacgatgat cgttacaata
    14041 gctgtactca acccgctggc accattcatt acgaaagaaa agagccaaga attcatcccc
    14101 catagtgcat acgctcccca gaaaaccact cctgcaataa aatgccaaaa atatctggaa
    14161 agtgcgccaa tcaatgcacc aaacacgatg ctttttctaa tttgtttcgt attatcgtct
    14221 acatagcctt tttggacatt tgaagccatg agccctgctg cgcctgcaaa agtataggca
    14281 atcgggtact caatcaacac ttgaacgaca cttaaataat aaacttgtcc caacggaaaa
    14341 tgtaggatgc cccaaatgaa acctgccagc atccctgcct ttgtccctct tcttaacgca
    14401 aaaagagtca atggaatctg ccctaatgaa atagaaaaac tgctgccgat ctggatcgga
    14461 atcaacgaca acaccatcgc taacgctgca acaatcgttc cttctaccca aactctattt
    14521 tttcccataa aaaaacttcc tcctttgaat aaaaaagtca caaaggagga agcagctttc
    14581 gaaaatagtc aaacagctgt ttattcacaa ttcctacgct cgcataaccg atacaggtac
    14641 gaagggttta gaaatagatt caatctcagc ccaattggac tcccctttgt gagtttattt
    14701 attatttatt ttttgtttca aacaatggac taactggttt gttttcatgg atacgtttga
    14761 ttgcatctcc cattagtccg ccaacactta cttcatcgat tttgtcgatt ttacgatctt
    14821 ctggaagata gatcgagtct gtaacaacta gacgctcgat agctgaatct tctattcttt
    14881 gtaaagcagg gccggataat actggatgtg tacatgaagc atacacacta gttgcaccag
    14941 cttctttcaa agcattggca gccaatgtga tcgtacctgc tgtatcgatc atatcatcga
    15001 tcagtacaca agttttgcct ttgacttgtc caatgatatt cattacttct gcaacatttg
    15061 cttttggacg gcgtttgtct atgatcgcaa tcggagattt caaaaattcc gctaatttac
    15121 gtgcccgtgt gacaccacca tggtctggtg aaacaacgac aacatcatcg cctttgattc
    15181 ctttttctaa gaaataattg gcaataagtg gcgcacccat caaatgatcg actggaatat
    15241 caaagaaacc ttggatttgg actgcatgaa ggtccaaagt cagcattctt gttgcaccag
    15301 ctttttgaat catgtttgct actaatttcg ctgtgattgg ttcccgtgca cgtgctttac
    15361 ggtcttgacg cgcatagcca taataaggca tgactacgtt gattgttttt gcacttgctc
    15421 gtttcagtgc gtcaatcatg atcagcaact ccatcaagtt atcattgact gggctgctcg
    15481 ttgattgaat aacatagaca tgcgatccac gaatactttc ttcaatgttt acttgaattt
    15541 ctccatcact aaattgagta acagatgatt ttcctaactc gacgccgact gctgctgcaa
    15601 ttttctctgc taatgggcga ttggaattta acgcaaaaat ttttaatctt gggtcaaagt
    15661 aatgttttga catggggacc tccactttct ttttcacaac tctagaattc tacctaataa
    15721 atagtagtca tattttttaa ataaatcaag tgattttgca aaagtcttag tttagataag
    15781 ggagtttttt tgcatatcct tctttattga cttgtcttgc tctcgcaatt gccagcgcat
    15841 attctggaat attgtctgta attgttgacc cggcagcaat cgaactgttt ttagctacct
    15901 cgacaggtcc gatgatattc gtgctagaac caataaagct gtggtcgccg acagtcgtat
    15961 gatgtttgtt ctttccatca tagttgacga agacaacacc acatccgaca ttgatttctt
    16021 ctcccagcgt agcatcacca acataagtca agtgaccgac tttagttcct ttgccgattt
    16081 gagcattttt cacttccaca aagtttccga tatggacgtt ttcgccaatt tcagcttttg
    16141 gtcgtaaatg tgcataaggt cccacatctg catgtttctt gacatggctg ctttcaatga
    16201 ctgagttttc aatgacgacg tgatcctcaa tcctactgtc tacgattttt gaatgtgacc
    16261 cgatgacaca atcagaacca atgactgtat ttccttggat ttgtacacct gcttcgatca
    16321 atgtgtctgg accgatttct acgcctgcat cgatgtatgt cgttgctgaa tcgacaaagc
    16381 ttacgccatt gaccatatgc atctggttaa ttcgctttct catgatctca tttgctttag
    16441 ctaacgcgat ccgatcattg actcccattg attcttcaaa atcatccgtt tgataagcag
    16501 caactgtatg gccttcttct tttaagattt cgatgatatc tgttagatag tattcacctt
    16561 gagcattgtt tgttccaact ttgcttaacg catcgaaaaa agcttcgttg tcaaaacagt
    16621 aagttcctgt attgatttct tgtacaagag cttcttctgg tgttgcatct ttttgttcca
    16681 cgattttttc tacgatccca atatggtcac ggatgattcg tccatatcct gttgggtttt
    16741 ctgcttgtgc cgtcaagatc gttgcacttg cattttttcc ttggtgatat tcaaaaagat
    16801 tgttcagcgt ttcagtagtc aataatggtg tatcgccact gataactaac gttgttcctt
    16861 tttttccttc caagaaagaa gcagcttgca aaacagcatg tcctgttccc aattgttctg
    16921 cttgtaacgc atatttactg cgttctccta gttgtgcttt aacttgttct gctccgtgtc
    16981 caacgatcgt aatcacttca tctggtttcg tttcactgac ccgattaatg atatgttcta
    17041 ccataggttt accagaaacg ggatgtaaaa ctttatataa ttttgacttc atgcgggtac
    17101 ctttccctgc cgccaaaata attgcataac gtgcgtccaa ctgcgtcact cctaaatata
    17161 gtttgctgat ttcagtttat cccagtatac ccattttttc aacaaaacaa gtgattttct
    17221 tttatcccag cgttttctgg ataaaaaaat agatagacca taacgggcct atctatttgg
    17281 taaccggtgc tctcctgcca actcctcggt cacaagtcac actactcata aaacatgaaa
    17341 agctgttttt tgaaatcgtt tcttgtgact tagagttaaa cggtaggctt acaacctctt
    17401 cattaaacgt ttttcaatca gctgaccttc ggtattagct caataaacgg caaaatatga
    17461 ggagcaattt ttccgtttat tgagctaata ctcaggacag gacgctgatt tcacaacctc
    17521 tagataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gaaaaaatgt
    17581 ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcggctt tttcacaacc
    17641 tcttattctg aaaaataatt tcctggcaca accgtaatat ttttcgtttg agtatctaca
    17701 tctttgacat aaagcagtga cgtataatca tcgatggcgc gatgaccgtt gaattttgat
    17761 tcggcaaata cagtgatgcc aacaagttct gattcgaatt cttcaatcaa gctcttcatt
    17821 ccgttgactg ttccgccgcc tttcatgaaa tcatccacta caaggacacg tgaaccacgt
    17881 ttcaaacttc tcttagacag ttccatcttt tcaattcttt cagaagatcc agacacatag
    17941 ttgacactta ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat aacgaatggc
    18001 gcattcagat aataggacac agcttgggca ataggtacgc cttttgtggc tacagtcatt
    18061 acagcatcga ttttttcacc aaggtattta gaagcaatga ttcttccaac ttgacgcagc
    18121 agttcaggtt ctcctaacaa atcagagaga taaacatatc ctcctggaag cagacggtct
    18181 tgttctgata gacgttctgc tagagattca atatattttt tcgcctcttc atatgaaatt
    18241 tcagggatga acaatacacc acctgcagca cccgggattg tttccaaggt tccataccct
    18301 cgttctttaa atgttttctt gatgattccc aaatcttcac tgatagaaga tttagcagat
    18361 tcataacgtt cggcaaagct tgttaaggaa atcaattcat gcgggtgatt caataaatac
    18421 tgtgtcatgt caactagacg ttcactgcga cgtactttca cctattccaa ctccttttaa
    18481 gaaattttat cgttttacat tatataagtt tttcttcaaa aattcgagca ttttatgaaa
    18541 aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa aatttttcgt
    18601 ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg caaccaatat
    18661 tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc
    18721 tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac
    18781 acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc
    18841 gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat
    18901 agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac
    18961 gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa
    19021 tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac
    19081 gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat
    19141 caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata
    19201 ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat
    19261 ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac
    19321 atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat
    19381 cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga
    19441 aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt
    19501 ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta
    19561 tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca
    19621 tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt
    19681 tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct
    19741 aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt
    19801 ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat
    19861 gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca
    19921 atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac
    19981 ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag
    20041 acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa
    20101 cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt
    20161 tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc
    20221 taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt
    20281 cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt
    20341 tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac
    20401 ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt
    20461 gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt
    20521 gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa
    20581 ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg
    20641 atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg
    20701 cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac
    20761 tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc
    20821 tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc
    20881 agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata
    20941 catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt
    21001 atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt
    21061 tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac
    21121 taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat
    21181 gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact
    21241 acaataaaaa actgaagaat accattttag gattcctcag tttttcatca cctattttac
    21301 ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc
    21361 cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc
    21421 ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga
    21481 tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct
    21541 gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg
    21601 cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca
    21661 acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga
    21721 caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt
    21781 ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag
    21841 ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa
    21901 agatcttgac accggttttg atattgtatt tattttttag caataaagca gcttgataaa
    21961 cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt
    22021 ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca
    22081 actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg
    22141 gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc
    22201 tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt
    22261 ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata
    22321 attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac
    22381 tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc
    22441 cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa
    22501 aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt
    22561 ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt
    22621 atacaataaa cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga
    22681 cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa
    22741 cgactttttg atctttacgg acaataccga caccgctaga taatacatat gatttatcaa
    22801 atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat
    22861 ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt
    22921 tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat
    22981 caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta
    23041 cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca
    23101 taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa
    23161 agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt
    23221 aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg
    23281 tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt
    23341 cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc
    23401 ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga
    23461 ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca
    23521 agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt
    23581 ttctggataa ccacacagct aacagaatca agacatatac caatatcaac tgccaagata
    23641 acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg
    23701 cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata
    23761 gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac
    23821 tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga
    23881 cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta
    23941 aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag
    24001 gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa
    24061 cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga
    24121 ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg
    24181 aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt
    24241 gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg
    24301 tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa
    24361 gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg
    24421 taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg
    24481 ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta
    24541 ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa
    24601 actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat
    24661 catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca
    24721 tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat
    24781 ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca
    24841 gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt
    24901 cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca
    24961 ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga
    25021 gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat
    25081 ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt
    25141 gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa
    25201 gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta
    25261 ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa
    25321 tcattaacag atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg
    25381 ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc
    25441 acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct
    25501 ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt
    25561 tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca
    25621 gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat
    25681 aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt
    25741 gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg
    25801 atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct
    25861 tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt
    25921 agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc
    25981 cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga
    26041 gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga
    26101 ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt
    26161 ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac
    26221 aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat
    26281 tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc
    26341 ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa
    26401 ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga
    26461 atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat
    26521 tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg
    26581 aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg
    26641 gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg
    26701 atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt
    26761 cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc
    26821 atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg
    26881 ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg
    26941 ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat
    27001 gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg
    27061 atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact
    27121 aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg
    27181 attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact
    27241 ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa
    27301 caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc
    27361 tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa
    27421 accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac
    27481 ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa
    27541 ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat
    27601 gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt
    27661 aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga
    27721 aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact
    27781 cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag
    27841 tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc
    27901 aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct
    27961 gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta
    28021 gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc accgctttta
    28081 tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa
    28141 ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact
    28201 ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt
    28261 acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga
    28321 ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt
    28381 gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac
    28441 ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact
    28501 gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga
    28561 ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt
    28621 tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt
    28681 ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca
    28741 tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca
    28801 agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata
    28861 gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga
    28921 tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc
    28981 ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat
    29041 cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg
    29101 atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa
    29161 atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg
    29221 gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc
    29281 acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa
    29341 ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca
    29401 gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca
    29461 gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg
    29521 tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct
    29581 tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt
    29641 ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg
    29701 accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca
    29761 gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca
    29821 ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa
    29881 gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga
    29941 ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct
    30001 tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca
    30061 tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga
    30121 gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg
    30181 atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct
    30241 agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata
    30301 tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa
    30361 aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat
    30421 aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag
    30481 agttagacgg caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca
    30541 caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat
    30601 aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca
    30661 gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca
    30721 tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga
    30781 caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag
    30841 tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc
    30901 ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa
    30961 ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat
    31021 ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta
    31081 atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc
    31141 gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat
    31201 tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt
    31261 cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat
    31321 tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg
    31381 gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt
    31441 gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa
    31501 acataggcat tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg
    31561 gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct
    31621 gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac
    31681 tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta
    31741 agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag
    31801 cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc
    31861 caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt
    31921 tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg
    31981 actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg
    32041 taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt
    32101 tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc
    32161 cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt
    32221 ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca
    32281 tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt
    32341 cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt
    32401 aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta
    32461 gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt
    32521 caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
    32581 ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact
    32641 tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc
    32701 ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    32761 tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa
    32821 tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca
    32881 actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa
    32941 tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag
    33001 gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg
    33061 atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg
    33121 gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc
    33181 aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg
    33241 actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat
    33301 tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc
    33361 gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt
    33421 ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt
    33481 acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg
    33541 ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt
    33601 tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata
    33661 ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg
    33721 atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt
    33781 agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg
    33841 atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg
    33901 atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc
    33961 gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca
    34021 ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc
    34081 accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg
    34141 aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt
    34201 ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc
    34261 atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat
    34321 ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc
    34381 cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag
    34441 cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc
    34501 atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc
    34561 atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata
    34621 ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc
    34681 aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt
    34741 ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac
    34801 gatattcact tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa
    34861 tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat
    34921 gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat
    34981 cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa
    35041 ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa
    35101 atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt
    35161 aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg
    35221 atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc
    35281 tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa
    35341 ctttgtctga tccgaataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg
    35401 tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt
    35461 gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt
    35521 tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt
    35581 tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg
    35641 atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag
    35701 cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag
    35761 ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg
    35821 ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct
    35881 tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag
    35941 gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg
    36001 ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag
    36061 cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc
    36121 ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag
    36181 acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct
    36241 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    36301 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    36361 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    36421 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    36481 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    36541 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    36601 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    36661 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    36721 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    36781 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    36841 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    36901 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    36961 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    37021 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    37081 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    37141 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    37201 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    37261 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    37321 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    37381 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    37441 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    37501 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    37561 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    37621 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    37681 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    37741 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    37801 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    37861 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    37921 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    37981 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    38041 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    38101 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    38161 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    38221 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    38281 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    38341 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    38401 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    38461 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    38521 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    38581 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    38641 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    38701 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    38761 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    38821 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    38881 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    38941 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    39001 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    39061 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    39121 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    39181 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    39241 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    39301 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    39361 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    39421 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    39481 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    39541 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    39601 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    39661 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    39721 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    39781 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    39841 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    39901 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    39961 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    40021 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    40081 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    40141 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    40201 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    40261 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    40321 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    40381 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    40441 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    40501 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    40561 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    40621 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    40681 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    40741 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    40801 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    40861 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    40921 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    40981 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    41041 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    41101 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    41161 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    41221 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    41281 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    41341 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    41401 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    41461 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    41521 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    41581 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    41641 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    41701 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    41761 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    41821 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    41881 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    41941 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    42001 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    42061 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    42121 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    42181 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    42241 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    42301 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    42361 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    42421 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    42481 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    42541 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    42601 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    42661 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    42721 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    42781 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    42841 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    42901 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    42961 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    43021 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    43081 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    43141 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    43201 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    43261 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    43321 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    43381 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    43441 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    43501 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    43561 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    43621 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    43681 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    43741 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    43801 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    43861 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    43921 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    43981 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    44041 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    44101 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    44161 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    44221 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    44281 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    44341 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    44401 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    44461 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    44521 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    44581 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    44641 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    44701 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    44761 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    44821 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    44881 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    44941 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    45001 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    45061 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    45121 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    45181 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    45241 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    45301 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    45361 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    45421 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    45481 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    45541 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    45601 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    45661 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    45721 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    45781 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    45841 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    45901 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    45961 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    46021 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    46081 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    46141 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    46201 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    46261 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    46321 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    46381 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    46441 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    46501 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    46561 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    46621 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    46681 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    46741 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    46801 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    46861 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    46921 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    46981 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    47041 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    47101 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    47161 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    47221 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    47281 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    47341 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    47401 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    47461 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    47521 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    47581 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    47641 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    47701 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    47761 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    47821 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    47881 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    47941 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    48001 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    48061 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    48121 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    48181 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    48241 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    48301 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    48361 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    48421 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    48481 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    48541 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    48601 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    48661 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    48721 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    48781 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    48841 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    48901 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    48961 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    49021 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    49081 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    49141 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    49201 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    49261 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    49321 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    49381 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    49441 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    49501 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    49561 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    49621 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    49681 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    49741 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    49801 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    49861 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    49921 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    49981 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    50041 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    50101 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    50161 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    50221 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    50281 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    50341 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    50401 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    50461 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    50521 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    50581 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    50641 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    50701 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    50761 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    50821 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    50881 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    50941 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    51001 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    51061 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    51121 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    51181 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    51241 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    51301 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    51361 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    51421 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    51481 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    51541 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    51601 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    51661 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    51721 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    51781 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    51841 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    51901 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    51961 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    52021 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    52081 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    52141 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    52201 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    52261 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    52321 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    52381 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    52441 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    52501 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    52561 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    52621 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    52681 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    52741 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    52801 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    52861 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    52921 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    52981 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    53041 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    53101 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    53161 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    53221 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    53281 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    53341 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    53401 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    53461 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    53521 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    53581 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    53641 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    53701 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    53761 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    53821 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    53881 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    53941 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    54001 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    54061 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    54121 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    54181 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    54241 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    54301 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    54361 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    54421 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    54481 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    54541 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    54601 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    54661 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    54721 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    54781 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    54841 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    54901 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    54961 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    55021 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    55081 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    55141 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    55201 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    55261 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    55321 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    55381 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    55441 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    55501 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    55561 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    55621 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    55681 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    55741 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    55801 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    55861 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    55921 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    55981 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    56041 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    56101 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    56161 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    56221 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    56281 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    56341 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    56401 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    56461 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    56521 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    56581 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    56641 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    56701 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    56761 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    56821 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    56881 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    56941 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    57001 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    57061 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    57121 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    57181 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    57241 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    57301 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    57361 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    57421 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    57481 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    57541 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    57601 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    57661 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    57721 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    57781 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    57841 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    57901 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    57961 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    58021 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    58081 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    58141 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    58201 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    58261 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    58321 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    58381 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    58441 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    58501 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    58561 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    58621 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    58681 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    58741 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    58801 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    58861 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    58921 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    58981 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    59041 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    59101 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    59161 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    59221 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    59281 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    59341 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    59401 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    59461 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    59521 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    59581 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    59641 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    59701 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    59761 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    59821 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    59881 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    59941 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    60001 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    60061 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    60121 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    60181 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    60241 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    60301 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataattata
    60361 aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg
    60421 tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg
    60481 aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc
    60541 aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat
    60601 tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa
    60661 aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag
    60721 gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag
    60781 aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga
    60841 tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc
    60901 gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat
    60961 gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat
    61021 atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc
    61081 gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata
    61141 tccattcaga accattttca ctgaattatt caagactggt gaatcattta cgactgcttc
    61201 tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca
    61261 aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag
    61321 aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt
    61381 taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc
    61441 atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc
    61501 tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt
    61561 tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt
    61621 agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt
    61681 tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc
    61741 agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg
    61801 attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc
    61861 tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag
    61921 tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt
    61981 cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat
    62041 ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa
    62101 tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc
    62161 tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa
    62221 tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc
    62281 attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt
    62341 atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg
    62401 gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc
    62461 gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta
    62521 ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc
    62581 agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc
    62641 aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac
    62701 tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc
    62761 cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac
    62821 agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc
    62881 tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga
    62941 tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt
    63001 cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc
    63061 tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac
    63121 agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga
    63181 accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt
    63241 agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac
    63301 aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga
    63361 taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg
    63421 ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg
    63481 gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat
    63541 tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa
    63601 cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc
    63661 attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
    63721 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
    63781 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
    63841 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
    63901 aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc
    63961 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
    64021 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
    64081 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
    64141 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
    64201 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
    64261 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
    64321 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
    64381 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
    64441 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
    64501 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
    64561 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
    64621 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
    64681 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
    64741 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
    64801 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
    64861 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
    64921 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
    64981 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
    65041 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
    65101 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
    65161 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
    65221 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
    65281 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
    65341 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
    65401 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
    65461 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
    65521 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
    65581 aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg
    65641 atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
    65701 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
    65761 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
    65821 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
    65881 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
    65941 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
    66001 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
    66061 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa
    66121 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    66181 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    66241 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    66301 cgggctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    66361 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    66421 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    66481 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    66541 cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa
    66601 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    66661 gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata
    66721 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    66781 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    66841 taatcgatca attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt
    66901 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    66961 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    67021 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta
    67081 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    67141 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    67201 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
    67261 tttgtcagat aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact
    67321 ttttctcaca ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag
    67381 tgattatcaa atatgtaaaa tcagctactt ttttcttaaa cgtttcttta tactaaatat
    67441 aaataagtgt ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa
    67501 acgttttgga acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata
    67561 caagcaagta cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt
    67621 gggatgaaaa gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca
    67681 aacacatggc tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact
    67741 cactttccga actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt
    67801 taacagagat tcttatagaa catccgcaat ttatcgaacg cttcaaagtt aagatgccat
    67861 ttcatgcaat aaattattca gataaattaa caactatcat ccataaatac gatcatatta
    67921 tcaagaaaaa caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc
    67981 aagaagcagt tgaatcgctc attcagcaaa atgcctatct tcccgcattc gaattgatca
    68041 atgaagtgat tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc
    68101 ttctgataga ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag
    68161 aaaaaaggca aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt
    68221 caaaacgcta ctccataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc
    68281 aaaagaaaaa aatagataaa aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa
    68341 aatagcaagt aaacgataac aaccatccta aatccacaag aaatacagca gaccttgctt
    68401 taaaaacacg aatcatgcaa aggatgaagt aaagaagact taacgcgtac ccaatattag
    68461 tcatttgact attaggtacg cgttttgttt tataagtcat gacaaatttc cttcgttgct
    68521 gggtagattt tccggcattg ttgatagatt ttatgatact tttctacatt ttgtaagaga
    68581 ggttccactg catctgtata ttggataaaa ttctgacaac aactttcagg tgtgtcgaac
    68641 cactctaatc tttaaagata aatattaaaa taacgattaa taatattaac agtctttatt
    68701 tttcaccacc aaaaacctat ctttgcatat actaaataga cgtatataca cagataggtt
    68761 tgtcttgtga aaaataatga ttatccttac caaaaaatgt ttgtttttct acatgttcgt
    68821 acttatcaaa aaatatgatc cttattgaaa ttactggcta tttatattcc aacgacttct
    68881 tgtgttaatt tttgttatct catagtaaat tatgtttcat tttcacttaa tttagttgtc
    68941 acgtgttcgt caattcgtaa aaacggtaaa tatataaaca cacttgcggc tataattaat
    69001 atttgtaaaa cagctgctcg ccagtctccg gctgttgcta aaaatcccga tataactggt
    69061 ggcgtagtcc aaggaatttg cactactaca tgattaatta atcccacagc agttgcaaaa
    69121 tatgctactg tcaaagaaaa aatcggtgca agcacaaagg gaataatcag actgatatta
    69181 aatacaatag gcaatccaaa aataataggt tcgcttatat tgaatatagc gggagttacc
    69241 gataatttag caaagtcctt ataatctttt ctacgactga agataaatat tgcaatcaat
    69301 aaagcaatcg tatttcccga tccacccatt accgcaaagg tatctttaaa ggccatattg
    69361 ataatatgtg gaatttgctc tttgtttgca taagctgcca tattttcttg catattctga
    69421 atcaaaaacg gatctaacaa tgaacctgat attactgctt gatggatacc aaatccaaaa
    69481 aatagattag caatagatgt aattaacaaa aatcctggta aaccagttgt aattttactt
    69541 aatggctctg taactatcgt ggtaattagc ttatttatat ccatattaaa caattgaatg
    69601 attataaatg acaataaagc aaacgaggta atcgtaataa tgattgggat taatacatta
    69661 aaagacttta aaacagcggg aggaatattt ccggatagct gaatctgtag tttcttatta
    69721 ttagaaagtt taataaataa ttctgtagcc gctaatccaa caaatatacc tacaaacatt
    69781 cctgaagctc ctgtatagga tataggtatc acacccgtca cttcaacagc tttatccgca
    69841 ttttcaggag taaacatcat ctgcataggt gttaatacaa caaatgtcga aatagcaact
    69901 aataccgctg caatatgatt tgtataattt ctgcttgcac acatatgata agatactgct
    69961 gctacaacta ataatgtagt tatgcttaat gtaccattgg caattgaagt accaatttgt
    70021 tgtaacgtgg tcaatgtact agtatcgaca atttccccca tgaatcctgt tggttctaag
    70081 atcactgaat taataagtgt cacaaatcct gctaacatca taaatggcat aaatgtagca
    70141 aacgcatctc gcaaagttct taaatggatc tgattcccca acttagtcgc aactatacca
    70201 aatttttcaa atacaatgtc cttcctgcta cttttgccca tattttttct cctttatgct
    70261 tattgtaatc gattactttt tccacccaaa taatacatca cttatcaaaa aaagtaaata
    70321 cttttaatta aatatatata tcttttttag tataaaaaaa taagctctga aaaaataatt
    70381 ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca aagtcttgat ataaatgtct
    70441 cgataccgaa tactcgaaag gtttaccaga cgatagagca acaactttgt ctatctcaat
    70501 cataaattca cctggatcta tttttaatat ttttgcgtca aattcattcg cagtatctcc
    70561 tttgatacgt atgtgcgaag tctttatttc cagacccagt tcatttgtga tataagcata
    70621 tacagatttc tttaaatgtt ttggttccaa gcctggtatg actgctaatg gcatgaatgt
    70681 ttgctcaatc gagtatggtt gacaatcaat aaaacggctt cgtacaatat tatatacata
    70741 gtcttttaca ctgatactta atttatcagc tacttttttc gatggaagtt caattgagaa
    70801 agtatggatt tctgaatcta cgtgttcttc accaaccacg ctaaacaaac cttcgtttgg
    70861 accaagaact cgtgagttat caattggtgt tctgacgact tcagtcccat acccacttct
    70921 acttcgcaaa actccttcgg aaacaagtaa gtctacgcct ttctttactg ttaacttgct
    70981 tgtatgataa ataactgcca attcatctcc tgtaggaagt ttctcaccaa ttttatactc
    71041 tccatttaca atttttttct ttatatctaa gtaaatttcc atatacttcg caattttttt
    71101 caatggtgac cctccaatca aaaacatatc ttcatctata ctttaacata aataaaccga
    71161 taaaatccac atcaaaatta tacacacaat aaaaaagata tatatattta aacaaaacta
    71221 tttactttaa gttgtaagcg gtgttatcat taagtggaaa caggaggtaa aaaaattatg
    71281 acaactatga aaaagctaac ttttccagat gatttttggt ggggttccgc ttggtcagct
    71341 gagcaagcag aaggaaaagg agaaacagga aaagcagaga ctatctggga tcgctggttt
    71401 aaagaacaac cttaccggtt ttatcaacga atcgattcct ctattacaac cgatcacatc
    71461 catcaatata aagaagatat agaactaatg aaaaaaactg ggcataactc ctttcgcgtt
    71521 tctatttctt gggcacgaat gtttccaaat ggcggtaccg gaaaagtaaa tcccaaagcc
    71581 atttctttct atcgaaaatt attttccgaa atgaataata acggaatcaa agtatttgcc
    71641 aacttatatc attttgacat gcctgctact cttcaagata aaggaggatg ggaatcacgt
    71701 gaagtagttg atgcttatgt tcattacgct gaaacttgtt ttaaagaatt tggtgactta
    71761 gtttgtcatt ggtttacctt caatgaacca cttgggccta ttttaggtac ttatcttgaa
    71821 gattttcatt atccaaacct cattgacttt aaacgcggag cgcaagcagc attcttcact
    71881 atacttgcac acgcaaaagc aatacaagta tttaaagaac tagctcttcc tagtaaaata
    71941 ggcgttattc taaatttgag tcctactttt cctcgtagtc aaaatcctgc tgatgtgaaa
    72001 gcagcagaaa tcgctgattt attttatacg cggagctttt tagatcctat ggttaaaggg
    72061 aatttttcta aaaagcttat cctgttatta caagaatata atcagcttcc tgaagattat
    72121 acgaaagaag atcttgtatg tatctacgaa aataccgctc aaattcttgg actaaactat
    72181 tacgagccac gtcgcgtcaa atctcgttct tctatggtgg atacatctag tccattttta
    72241 ccagagtggt ttttcgaacc acatatcatg cctggacgac gaatgaacga ataccgggga
    72301 tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg atatcaagaa ccattatgga
    72361 aacatcgaag catttatctc ggagaacgga atgggcgttg cggatgaaga acgtttcatg
    72421 gatgatacag ggcaaataat tgatgattat cggattgagt atatcaaaga tcatcttgct
    72481 tacttatgga aggcaataaa tgaaggatgt aacattaagg ggtatcatct ttggacattt
    72541 attgattgct ggtcatggat caacgcctat aaaaaccgat atgggctggt ttcactcgac
    72601 cttactactc aaaaaagaac aattaaaaaa agtggcgaat tttacaagca attaagtgat
    72661 acaaatggat ttagatacga tgtagacttg ttagttgaat aatattctaa aattccactt
    72721 gaagtgaaca cattagaaaa caagaataat gagaaatacc aataacaaat actttttctt
    72781 catacttcat ttatacttgt cagtataaga gactgtgaca aatgtagtga tcctcagaag
    72841 ttaggtctgg aaatctaact tgagaagatt tttttaaact acgtccagtc ttttttattt
    72901 gtttaaaatt gaagttgaca agtcttcatt aaaaatttag aatattcaca agtttgacga
    72961 caattatgaa aatttatgtc gtgctaaagg agttttgtta ttggatgaaa agttagatat
    73021 aaaaactgcg atcaaagaca cgttacctac cgtttttggt tatatcggta ttggacttgc
    73081 atttggtatc gttgggaaag ctgccgggtt tcatccgctg gtcgtcacgt tgatgtccct
    73141 gctgatctat gctggttctg cccaatttat cacggtcagc atgcttgcta gccatagtcc
    73201 actgctctcc atcgttctct cgacctttct agtcaattcc cgaatgattc tgatgagtat
    73261 gacgattgct ccttatttca agaaaaatcg gctgcttcaa aatctgctga ttggcacatt
    73321 gctgacagat gaaagtttcg cactgggaat gaataaattg aactatactg gacaaaagtt
    73381 aaactttcgt tggatgaata cagctaattg gatttcttat ttgacgtggg tgggttcttc
    73441 tcttgttggc gcacttttag ggaattttat tactgatccg aaaaagtttg gtttggattt
    73501 cgccatcgtc gctatgttta tcgggttact ttatcttcaa gtcatttctg atagaaacac
    73561 ctctaaacga ttacagttga tcctgattgg tctcactttg attctggtat atgtcggact
    73621 gatttttatc ccaagtaact tagtgatttt agttgtaaca ctgattgggt gcggattggg
    73681 ggtgtggatc aaacatgcct tcttttgagt ttacattgct gactatttta ggttgtactt
    73741 tagcaacttg gctttctagg atcctaccat ttgtcttact gaaaaaattc gatttgccgc
    73801 aaccgttgct tgagtattta agttttgtcc cgatcgtcat tatgtctgct ttatggttca
    73861 gcagtctatt tacccaaaat atcggacatc tgccgcagat caatctcgaa aatgctttgg
    73921 cttcagttcc tacactttta gctgctattc tttcaaaaag cttattggtg atcgtcttgg
    73981 taggaattct ttccctttcg ttgattcgct tattataaat aaaaaaacaa gagcttatgg
    74041 caaattactg tcatgcgctc ttgtttttat caaagtcaaa taagaaaaaa atcggcgtat
    74101 tcttgcttat ccgttgaacg gaccactaac aacgactttt cctaatatat gtcctgtttc
    74161 tacgtctttg gttgcttgtt tcaagttctt cgcattcatc ccatctgaat agaccttgct
    74221 caatgtactg cggataatcc cttcatttgc taaagcagca atgtgcgcta aagcttctcc
    74281 ttgcgtttcg atttcatagt tatagtctgt tttagcgaac atatactccc agtcgaagct
    74341 gatagattgg tttttccaaa cacctatatc taacggctct ttgatcccta cgatcgttcc
    74401 cacatgtcca aatggtcgga caaggtgttt gatttgatca aagtagcggg taatatcgaa
    74461 taatacagcg acatactcta cttgatcgat tcctaattca cgaaaacttt tacttaagtc
    74521 gtgatgataa tcgattggat agtctacacc tgtttttttc agccattcaa aattcttcgg
    74581 acttgcagtc gcgtagactt ctaggccagc ccaatgcgct agttgattca aaatcgaacc
    74641 gacgccccca gctccgttga tcactaagat ttttttcccg cggttggcat tttcttctgg
    74701 agtcagacca aatttttcaa ataacaattc ataagcggtc aatgaggtca acggcaaggc
    74761 tgcggcttct tcatctgaaa gattttttgg tgcaagagcg acaatacgtt cgtcaaccag
    74821 ttggtattct tgattgcttc ctgctcttgt cgttgtacct gcgtaaaaaa cgcgatcgcc
    74881 gacagaaaat ttttgcactt catcacctac agcaacaact ttcccgactc catcaaaccc
    74941 taagatacgt aaagcatttc ttatcccgtt atcttggcga agtttcgtat cgacaggatt
    75001 aacagatact gcgttgactt ttaccagaag atcccgtgaa ctgggtacag gtatctgttc
    75061 tttttcatca ataaagcttt ttggatcatc gatcgacaac ccttcataaa aaccgactac
    75121 cttcattaat tccttttctg acatacgaat tcctccttag cttcatcaag ttggagcatc
    75181 catacagttt tcatttcaat atatatagta tgctatttgt tccactttta ttcatcataa
    75241 ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga ggttgcgaca
    75301 agacttttgt cacaacctct tttaaacggt gttcaatcaa ctgaccctcg gtattcgctc
    75361 aaaaaacgac aaaatatgag gagctatttt tgtttggctg ttcttattac ttgatgcagg
    75421 acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg ttcactgaaa
    75481 agcgttggat gttctttttc aaaggtgtct aagatctctc gtacttcatc agtagatttc
    75541 gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat atagatttta
    75601 aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg atcttgcaga
    75661 tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct cggttctttt
    75721 tcaaatgcat aaggattctt gaaaatcccc cggccaatca tgatgccatc tacgccatat
    75781 ttttcggcca attcttctcc tgtctggcga tctgggatat ctccgttgat cgtgatcagt
    75841 gtttgcggag cgatttggtc acgtaaagca atgattttag ggatgacttc ccaatgtgca
    75901 tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc tatatcttga
    75961 tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc gatacgcgtc
    76021 ttgacgctga caggcaaacc gccagcttta gccgcttcaa tcaattgtgc cgctacttct
    76081 gggcgaagga tcaagccact gccttttcca cgttctgcta cattagggac tgggcagccc
    76141 atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat ttctctaaaa
    76201 aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc tgtaaaggtc
    76261 aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga gtttgtaaac
    76321 tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa tacaacatct
    76381 gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt ccaaaaatta
    76441 gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat ctttctcaaa
    76501 aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt aaaagatgtt
    76561 tctgattgag caaaaaaaga ctgtgacaaa aatcttatca caattccttt tccggaataa
    76621 atggtgttca aaagcttgca tctgcggtaa taagtccaac caaacgaaaa ataggaggag
    76681 ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac aacctctcca
    76741 ctaaacggtg ttcaatcagc tgaccctcgg tattagctca acaaacgaca aaatatgagg
    76801 aactattttc ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    76861 ttctaacttc tcaatcctct tcctttattg atcaaatacc aagtaacggc gtacaaaaca
    76921 acgatgaata gcacaagtac gatcatcgat gttgctactg gcacatcgat tgttccaagg
    76981 aatccgtaac ggaaaccgaa gatcatataa acgatcggat tgactttcga aacagcttgc
    77041 caaaatggcg gcaacataga gatggcatag aaaacgccac ctagataagt taacggctgc
    77101 aagacaaatg taggcacgat cgatacatcg tcataagact gagcaaaaat cccgttgagc
    77161 agtcctgcta acgaaaacaa gattgccgtc atcaataatg taatgatgac catgaaccat
    77221 gaatacacat gaagtggtac aaagaacaag gaaatgatcg ttacgagaga gccgacgaga
    77281 atactgcgac ccactccgcc aatcacaaag ccccaaatga tcacatgcgt aggaacaggc
    77341 gctaccaaga tttcttcgat gtttttctgg aatttttgag agaagaagga cgaagaaacg
    77401 ttggcatacg aactagtgat agcagacatc ataatcaaac caggcacgat gaattccata
    77461 tatgaaaaac cacccatgtc accgatccga ccaccgatca tttttccgaa aataacaaag
    77521 tacaaggatg tcgtgatcac aggcggaacc agtgtttgga cccagatacg caaatagcgg
    77581 ttcgtttcct tgatagctaa actttttaat gcagtaaaat aaagattaaa catttttctc
    77641 ctccgtatga ttattttctt ctgtgatttt caaaaacagt tcttctaaac gattcgactt
    77701 attcctcata gaaagaactt tgattccttg tcttgtcagc tgatcgaaga ttccgttcac
    77761 cccttgattt cgttcaactt cgacagcgat tgtctgttca tcttcaaatg tacaagcgta
    77821 tccttcaatc acaggtttgg tctcatatgg cgctaaatca aagataaatg tttcaaattg
    77881 cagtttggat agaagctctt tcatactagt attctcgatc agttcacctg attggatgat
    77941 cccgatgttg cgacaaagca tttcagcttc ttctaaataa tgcgtcgtca aaatgatcgt
    78001 tgtaccattt tcgttcaatt ctcggagaaa ctcccacatc tcgcgtctca attcaatatc
    78061 gactcctgct gttggttcat ccaagatcag caaacgtggt tcatgcatca atgccctagc
    78121 aatcatcagt cttcgcttca ttcccccgga aagcatacgt gcacggacat ttctcttttc
    78181 ccaaagattg gactgcttca aatatttttc gctgcgtttc atcgcttctt tgcgagagac
    78241 accgtagtag cctgcttgat tcacgacgat ttgctggacc gtttcaaagg gattgaaatt
    78301 gaactcttgc gggactaatc caatctgttg ctttgctcgg actagatccg tatccagatc
    78361 ataaccgaag acattcactt ttccagatgt tttattgact aaagaagtaa tgattccgat
    78421 cgttgtagat ttcccagctc catttggtcc taatagcgca tagaaatctc cttctttcac
    78481 ggaaagatcg attcctcgca atgcttcgac cccacttcca taaacctttt tcaaattatt
    78541 tatttcaagc gcatttgcca tttatttcct atctccttat agacatgatt ctttattgta
    78601 tcatggaata cagcagatga aaaaacaact tggttccagc gaaaaatcag gttgctgggc
    78661 tcaggaaaat ctcctaatga aagacatcaa tcggacgaag tcagacaacc ttttcagaaa
    78721 aaacaaatcg aagataaagg attactattc atatgtgcaa caaaaggagc gtgagacaaa
    78781 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag caaaagccaa
    78841 gatttttgat ccaataaata ctttcgatca cttcgcctat cccaatacat gccactgccg
    78901 cttttcatca aataacgcgg cttccgtttt tgcggtttct actaaagtct ctgggtaatc
    78961 caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa ggtccttctt ttaatttgaa
    79021 atcaaaactg attccttttc cttctgttac ttgttcttca aagtggacat tctcacaata
    79081 attctttaaa atctctgtca attcgatatc atgtgtggcg acaaatgcta aagatggata
    79141 ctccgcaagc caccgaacga ctgaagcgga agaagcaatc cgttcgatcg tatttgtccc
    79201 ttttaaaatc tcatctacga agcagtagca gcgttctttg gaagccactt ggtccaacaa
    79261 acgtttgatc gatttgattt ctgaaacaaa ataactgtcg ccttcaaata aatcatcttc
    79321 gattgccatg gaagtcaaca catgtccagg ttgcagggta aactgttctg caatcgctgt
    79381 ttggatcgtc tgtgctagaa tacagctgat ggctatactt ttgacatacg tagattttcc
    79441 tgatgcattg gaacccgtga ctaatgtatt cttctgccag ttgaccggat tcactaccgc
    79501 ttcttttaac aatggatgat agatatcttt cgctgtcaca ccgccctcag taaactctgg
    79561 tcggcaagtg atgggcatat aagtacggta attcaatatt gcagcggcta cttctaattc
    79621 tcccagcaat tcccacagct tgatggcttc agttgaatgc ttttctaatt tagtgagaac
    79681 gaaatgatag gagataaatg gcagcatgaa catgatgttg acatattcaa aaatcaactc
    79741 tgcttcactc ccactcttca tacgaaatga aaaacctaat ttagggatgt ctcttaatgg
    79801 tttagtttgc tgtttcagct cttcttgaag tggtgtcggc agttttgcta gctgattggc
    79861 ggctgaaatc gttgaaacca aataactcat gctattcagc tctgtttcta gtgtttgttt
    79921 cttcactaga taatagaccg tattgaatac taacccaccg attgccaaat agattcctag
    79981 tacttgtcct aaaagaagca aacccaaacc caccagcgga agcaagccaa gaaaaataaa
    80041 taaacctacg cgtccaatct ttttactttt tccatccgct agatattgtt ttgcaaaatt
    80101 atgatctttt tttcctaaac gagcgaattg atattggatt ttttctctca gttgaggatt
    80161 ttcctgataa aaagcaatca gtttttctaa ctgctgatct tcaccaaaat caaagctgcg
    80221 taaacgttga tacaacgctt ctgaacctac actagaatag gtactgttga ttccttcgaa
    80281 tacttcgaac atatccaggt cataccaagt aagatcatcg atttcgctat cccatgaacg
    80341 gaacttcttc tccgtgagcc atgcttcctt caagctttct tctttatcaa atctcggttg
    80401 ataaggaatt ttttcccatt tgctacgaac tgtttccttt aatttcaaac gtgaaaaaat
    80461 gtctaaagca actaataaaa taacagctgc aataataatt aaaacgataa attctctttg
    80521 acccatatcc ctctccgttc ctttatttgt tagctttagt atagcactgt tgcgctattt
    80581 gaaaagaggg taaaaaaaca cctttcaaaa taaaaggtgt ttttttcttg tatattacat
    80641 atttatctct catttatcaa aattgacaaa taatcatagg gaggaattgt tgttaattct
    80701 tgtttattct ccagtgcatt gacaactgtc ccaagggaaa ggatagcctc aggtactatt
    80761 ttttcttgtt ctgttggatt agctaagcga agaacaaatc cttgttcatg aaaataagaa
    80821 ggataaatcg atgatacaat taagtcttca ggtaattcaa tcaatgaaaa tttacgagga
    80881 acatcaagtt cattttgtaa tggccaaatc ttgttatcta acctgtgaat aaatacattc
    80941 agtttctgct tttgataaga aatagattgt tctaaccgtt tttctgcctg ttttgccact
    81001 ttgaactcat taaatcttcc ttcttctaca ttaaatccaa aagtgacttt atactcacct
    81061 atctcttgag ctagcggtgt ttccatcatg acatgccctt catttgtcgt atctcctgaa
    81121 gctcgcccag gtcgccaagc aagattaggt tttccaagtt cccctgttgt tgaaaataag
    81181 gttatgatta attgattttc tatacgttca tactctttta aaccatctgc ataaaacgta
    81241 aaataatgat cattttctgc cacagagaca ctcttatcga atatttctaa attaacaggt
    81301 ttctctacca tagtttctga ccaattcgga ggaatgacat caggggtatt ttcaataaat
    81361 ccgttttgaa tttgtgcaat tgatttttca gatttatcta aaacattaat tgctaagcgc
    81421 atacgatgcg agtaaacagt attatgaatt ttgagtgtgc cttcaacgat ttgactttct
    81481 cccagactga acgatatttc ataagatatt tcttccattt cgggtttttc agataaacga
    81541 tcttttaaat ctttaggaag ttgagctttt ccgtaaacaa ctaatgtttc ttgtaaagaa
    81601 tctttatata ctttagctgt ttctaatttt agagatagtt ctgtatctcc ttctaatggt
    81661 gaataatcat acgtatcgcc atcatttgct gaatcgatca aatgaacgaa atcaagatat
    81721 tgtctatctc caactatcaa attcaattgc ccatctttaa aagaaagttt atatttattg
    81781 ttacttatct cagttttgtc tatctcgatt gctgtttcta atttttcatt atttttctca
    81841 aattcaacaa cagtgtatcc tagtgctggc acatttacat ttatttttat gtcaagttca
    81901 taataagctg gttcatcaaa atattcgaaa cctgtagcaa tcatccgata agcatttttc
    81961 cgttctggaa aataaacttc tttttcaatg atattatagg gactaccttt gaagtgtatt
    82021 ttctttgaat cagctactac atgcacgatt ttcgatccac tataattctc agtcgttgtg
    82081 ttgaaaacaa ggacttcatt cgattttagc tttaatgcat cagccatccg ttttacaata
    82141 aggttttcga ttccgtcagc tatctcatta gcttctttga ttcgatggaa aatatcttcc
    82201 gcaacattat ctgaaacaca gccaccaata ctatcatgag cctggttttg caaaatcttt
    82261 ttccataacc gacgaagtag gcctttactg atatggatac catttctttc tgcaatcagc
    82321 atgagcggct caactcgttt tagaattttt tgttccaatt taaaattttc tattttcatt
    82381 tttgttctta ctgagccaat agaacgatga acacgagagt aaaccggctg acgaagttca
    82441 cctttatatt caggtaattc ttttttgttt ctgataactt ctacataaga aggataatct
    82501 gaaataatat tgtggaattt actgtattta ttcaacttct ctacgacttg atcaaaattt
    82561 aaaatcattg acttttgatc tacacccgaa ggtactaaaa tatctttgtt ttttccttgc
    82621 atatgaagca tttcaactgc ttcatccaac ttttcttttg caaattcagg aatatcttta
    82681 attgcgtcta aggtcatcat tccagtcata taaccaaatg gaaaattcat ggcataaaca
    82741 aatttatctc ccaatccttt ccagataaaa taaggagatg gaaccatcgt ttcaaagttt
    82801 aatcctcgcc aacacatgaa attatcgatt tctacttgat gcaaaagtgt aggcaaattc
    82861 gcattgaacc caaacgtgtc aggcaaatac cctaccatca ttggatctcc atatttaagg
    82921 atcgtatcac tgattccaat cattaagttt cttaaaattg attcagcatc cactaataag
    82981 gcatctgttt gtgcatacca cggtcccacg aatagttgtc ctgttttcat cagtcgttgg
    83041 ataaccttaa gcttttgcgg attgatctcc aaatactcat cgacgattga agtttgtcca
    83101 tctaaacaaa agtttacttt agggttcctt tccaattcat ctagaacttc agtaaagacc
    83161 tgatcagaca atacgatggc atcttcattt gtaaaatacc attctctgtc ccagtgcgtg
    83221 tgttgaatta tatgtgcatt taccatatca gattctccta ctttccttct tctactgaat
    83281 ttacttgttg ttctttttta tacttgatgg attttagtgc attgaccatt aacgctgtaa
    83341 ccactccccc aataacaatt gatactaaat ataaaggtat attcgtaaca aaaggaattg
    83401 ccataaaggt tgaaagcatt gtttcttgag taacaccaaa aaatccattt agaccacatg
    83461 ctactgcagt gcccaccacg atgcttggta ttactcgtaa aggatctttc acggcaaacg
    83521 ggatcgtccc ttcagtaatc cccacaaggc ccataatcat agctgtcttt gcattatctc
    83581 gttccgattc atcataaagt tttttgttta aaattgttgc taaaaacatt ccaaatggcg
    83641 gaatagcaat tcctaataac atggctgtac taggtccaaa gcttccgctt gaaaaagaag
    83701 caaccccaaa agcatatgcg attttattga taggtcctcc catatcaaac gccatcattc
    83761 ctcctaaaat agccgacatc aaagcagcat ttgctcctga catgccttct agccaattag
    83821 tcagtcctgt tgtcattgct tgaataggcg tagctagtac ataccataat atgattccaa
    83881 caactaatgt agtaacaaaa ggaatcagca taattgattt tagactaata acttgcatcg
    83941 gtacttttac ttttttcaat gcattagcta aatagccaca agctaatcca gcaaccattc
    84001 ctccaataaa tcccatacca agattttgac acatcatccc agtgataaaa gctggtgcta
    84061 gaccgggacg gtcagcaatt gaatatgcga tataggctga aatcatcggt acgataaact
    84121 gtaatcccgt ctgaccaatc attcgcagga ctccccagaa tgtatccgtc tggtcccaaa
    84181 cacctgtgcc accaaaaatg actgcaatag aagttagtaa ccctgctgct acgacaagcg
    84241 ggatcatata ggaaacccca ctttggaaat gatttagaat ttctcttcct tgtttcttga
    84301 attccattag taactccccc acgatatatt ttttatttgt taattaatgc aattgcttca
    84361 tcaatcgtct cttcaccatc ttttactacc tctgaggtcc ctactgttaa aatcggttta
    84421 ccgtcaaagc gacctcgttc tgaaacattt gtatctactg caaatatgac aacatctgcc
    84481 tcggaaacat ccgaaattga aagtttgttt tctatcccca tagccccctg tgtttctact
    84541 ttaatatcta accctttctt cttcgcaaac ttttctaaat tagcttttgc catataagta
    84601 tgggcaatcc ctgcgataca tgctgttact gctactattt ttgtcatatg aaattcctcc
    84661 ttttaaaatg atagagcttt taccaattct tttttatttc tcgaatcttt tattctttta
    84721 acaaactcgt catccatcaa tcttccagat aattctgcta aaatacgtaa atgattagta
    84781 cctttatcag tgtcttttat tgctaacaaa aagacgagat caactggttg atcatctaat
    84841 gcgccccatt caacagaccg ctttaacttg gctacagcca ctgttgattc aattactgct
    84901 aggcttttgc catgaggaat ggcgatattt ttcccaattc ctgtggtcat atggttttct
    84961 ctgtcaaata cgtctttttg aaattgtttg tggtcagaca caaacccatt agtctctaac
    85021 aatttcgata atttttcgac tacacttttt ttatcagatg catttaaatc tatcactata
    85081 ttatttatat tgattttttc actgatcatt tataagcctt ctttcaatat ataaactaat
    85141 ttttcaaacg acgaactcgt ttttaaattt gctaataact catgattcga agcgatttca
    85201 ttgaatgttt cataaatttt attcaattca ggttttactt tttcattaag tgcaaaaaag
    85261 aaaacagaat gaacaagtgt cccatcccaa tcaattcctt ctggattaac gtagacacag
    85321 attactggct tttttataaa atctacttcg atatgcggca tagcaaaatc attcatcgct
    85381 gttgaagata gtttttctct tttgattgct ccctctagta tcccttcttt cgcgtaattt
    85441 tcttttacca attttttggt aattatattt aaaacgttca cttgattatc ctttatttgg
    85501 tgggtaaaaa agcaaaatcg tttatctaat aaatttagaa aagcatcttc tgtttgttca
    85561 tttaactgta gatactgttt tacttttctg acatctgccc ctaacatcaa aggactaaca
    85621 acaataacag gaaaatttgt attttcaatc ggaatcgtgc ttataacaag ttcatcagtg
    85681 acttttctat cttgtagttc attgatgctt agtacatctt ctatctcaac catatctgca
    85741 aaaccgttat ttattctttg ctctaataat tttgatgttc catatccgct actacaaact
    85801 aatatcactt tcgttttgtc aggtttgtaa cgatccaaaa gtgattgaat atggagtgtt
    85861 acgtaagcga tttcatcttc attaaaatgt atacagaatc tctcttcgat ttcctctaat
    85921 aaatctacac ttatcataaa tgacctttta aaagaatatt tgattttgtc cgtataaggg
    85981 ttatagatat ttactcccaa tttgagtctt ttaactgctg cattgagatg taatactaga
    86041 ttattgatca attcttcatc aggataaaat tcatttaatt taattcttac taactcttgt
    86101 agctcaacac ccatatctaa ataggaaagc ttcgtttgat ctaagtcgtg attcatagca
    86161 tttttttcaa tcgaagtaat gtgaatgtca acataagctt gttcatctat tggcacaata
    86221 attgaaaatg ctttttcaat cattttgatt aaaataagtg tattctttga gatttcttct
    86281 tcagaattat tactacgtga taagaaattg cttgtttcaa tcctttctat cgcaatagca
    86341 aggtggatag ctaaagtttg aaattcataa tctgtaaatt tcaattgtgt cttattcatg
    86401 tatcgattaa gcaattcggc tacttttttc aatatgtcct tatttaagaa attattcgct
    86461 tcaccaatga aattgatatt caagtctaag agatctgttt tactgttaga tgctgtaaag
    86521 ccaccccaat aatagttcac aacttctgac atcaaccttc ttttactttt ttcatcagct
    86581 ttgatgcgca cgccatttcg atctgaaact aaggacatcc catgcttgtt tagtcttttt
    86641 ctaacatctt tcattgtgct ttctaccgtc gacctgctca cgtataattc gtcagcaaga
    86701 ttttgaatct ttagagaatc attagtattc aataatttag caaacacata agttagacgg
    86761 tcctccggag agtctgcgat attgatttta tgactggata ataaatttcg aagtgtttct
    86821 ttttttccaa taagcgaaac gcctttttta ggttgaacca acatttgtac atctggcatt
    86881 ttttggatta tttctttcaa aattaaaata tctttgctaa ttgttttcgt agatacatat
    86941 aattgttctg ataattcttt gtaagttgtg tgctcatttt caatcaaaaa gtatagtatt
    87001 ttcatttgcc gttcgctaaa ttcgatgccc attatcttca cctcctgaat acagcttaag
    87061 tgtacaatta ttatctctat tttcatacag aagaaagttc ttgattaatg tagaagtttc
    87121 taatattctt gattatttca aatcaataaa aaagcctgca atatttatat cacaggcttt
    87181 tttctctatt aaatatatat aagataatac atttttctac tagttctcac taatcttgta
    87241 taattaaacc ctccgcaact tgacggagat ggagaagatg cccccgtcta atctagcggt
    87301 catctctcct cccattcctt ccaccaattt ttttgacagg taaagcccga ttccgctaga
    87361 ttccgtattt gtgcggctgg tattttcggt ataaaaacgc tggaagactt tttcaacaga
    87421 ggtttgcgtt cctggtttga taggattggc cactgttaat cgaatgaatg gatcctcttt
    87481 tgaaaatgtc acctttgctt gtccatctgc ataacgcaat aaattctgta tgatattttg
    87541 cagcactcgc agcaacatga gcggatctgc taatacagtt gtatttatcg agtgttcagc
    87601 gaacgctacc tcgatatttt tctcagaaaa ttgttcataa aaagagaaaa atacttcttc
    87661 cacctcttta gaaaaagaaa gcgtatggag atccatttct ttttgcttcg tctctattcg
    87721 agcaagatca taaaactgat ccgtcatact gagtaatctt gcaacagatt gtttgatgat
    87781 cgttaaatac ttctcccgtt tctccgtatc ctctacctgt tccagtaatt gtacataacc
    87841 gttaatagca gtcaaaggag ttctgagatc atgagaaagg ccgacgatcg atagtctgat
    87901 gttttcttcc atttcttgaa atgcttgtcc tttttgttca ttttcatcca accatgtatt
    87961 cagtgcatct actaggtcca ttagattctt ttctcgaaat tcgactgaaa gacgaccacc
    88021 atattcttgt cgttcagaaa gagtggcgat tttcttagtt aatctcttga tttccctttg
    88081 caggaagaaa ataaaacttc ccataaagat aacaacgaga agcaacagcc atttaatcaa
    88141 atctaaaccc cactccccac accgtatgaa tatacacagc atgtgccttt gctagcttcc
    88201 ctcgaagatt ggaaatatgc atattcactg ttttctcact atcaaaatat ggttcttgcc
    88261 aaactgtttc ataaagattt gctcgcgaaa atactttttt cggatgttct aaaaaaagct
    88321 tcagcagatc gaattctctt cctgtcaaat ggacagccac accattcaca tttacatccc
    88381 tcgactcttt gtccagtaag atttccttgt aatagatgcg ttgcgacaga tcttttactt
    88441 ggacagaagt ctgatgacga agttggatgc cgattcgtaa caaaagttct tcgatatcaa
    88501 atggtttcgt cacatagtca tccgctcctg ctgccaacaa ggaaacttta tctctttgat
    88561 cgctttttgc tgaaagaacg atgatcggaa cattcgatgt ctgtcggatt tctgctaaca
    88621 atgcttcacc tgaaagccct ggaagcatga gatctagaag gatcaaatcg acaggatatg
    88681 tgcttaacaa taattttcct tccgtacctg aataagcatc aagaagtttg tatctctgtt
    88741 caagaacctc ctccagtaag gaatgaacag cttcatcgtc ttcgataatc aaaatagttt
    88801 ccagcaggac cacctactta atgtcagatt tttggaaaat aaacattcct agagctgatg
    88861 ataccacgat tgttactaga ctgaccagaa tcattttcgg tgcttgcgaa taagttgctt
    88921 gttgcgcaaa gtccgtatac atgagccacg tttccgtaaa ccactccgtt agaaattcca
    88981 agcctttgat ttttgcgaaa acaccgggaa tgaaggccgt tcccatgata taaagaaaag
    89041 aaagaagcaa actgacactg gtacttctag tgatgaatgt caaagccact ggaacagata
    89101 atgtccctaa tgtcagcaga agcaatacag gaaatacttt tagaacataa tttccatagt
    89161 ttttgattcc ctcaacttca ccaaatccga aagcaagtgt gtaagcaagc atggctagtc
    89221 ccgtgatcaa aaccacgcca agtgttgtga tcaaaagcgt aacgagaagt ttcgacacat
    89281 aataagtcat tcgattccgt cctaaagata acgtattgcg aatcgtaccg ttttgaaatt
    89341 cttccccaat ataaaagcac gcaaatgctg cgaaagccag aagaatccat gcagccattt
    89401 tttctaccat taaaatccct gttgcaccgt tgatgactgt atccgttgct tggtttaatt
    89461 cattgccgta ttttgtatgc atataccatt cgttccaaga ataaaaaagc ggaagtacga
    89521 ccatcagtcc tagtgagacc cacattttac ggctcttcca tacttttatt ttttcagcag
    89581 ttaatagatt aaacatgtgg attgcctcca gttaattgta agaaataatc ttccagtttt
    89641 tgcccaacta ggtgaatacc atccacagga acttttgctt ctaccaatgc ttgattgatg
    89701 gctgctactt ggtcaattcc ttcaaaaagc tggataacac ggctagattg tacaaaataa
    89761 tccctataac caagagagtc gagaaccgtt actgcttgtt ccgtcgccga agtgtcaatt
    89821 tttataaatt gacgagattc ttgtgccaat tcttctttcg ataattcttt gatcaagtgt
    89881 ccttcatgca aaataccata gtgagtggcg atctgtgaca attcatctag caagtggcta
    89941 gaaagcaaga cggtgatgcc ccgttctttt accaaacgtt ggatgatttc tcgcatttct
    90001 gccatccctg ctggatccat cccattcgtc ggttcatcca aaatcaaaaa ttctggattc
    90061 gtgatcagtg catttgcaat agctaaacgc tggcgcatac ctaaagagaa attctttgct
    90121 ttctttttcc ctgtattttc caatcgcatc attttcagca ggtcttcgat ttctctatcc
    90181 gacacaccgc cgttcgctgc ttggatacgt aggttatctc ttgcagttaa ttctggataa
    90241 agagcagggg tttcaatcga ttgacccata cgtcgtcttg ctgcctgcaa atccgtcgtt
    90301 ccaaataatt cgatcgaacc ttcatcaatc gagatcaaac ccataatagt tctcataaag
    90361 gtagacttac ccgctccatt ttccccaatc aatccataga tcattccacg tttgatttcg
    90421 attgatactt tatctaatgc tttggcagca ccgtattttt tggttatacc agttgctttc
    90481 aagaccgttt catttccaaa atccatactt atccttcttt cttattgatg agtgaagtat
    90541 agttgacggg tataaagaaa gcagccagaa atgtataaag aaagtgtaaa ttttccaatt
    90601 gaatgtcaaa aaaaacaatc ggacaatacg tgtctttcct attatcaggc agtttgttat
    90661 acgcttgtat cagtttcctg attctttctt ttcgaccaaa gtcgtttctc tgtattcctc
    90721 ttcactgaaa atttttgctt ctttcagatc aagcttgagt aattttacca gaccggttat
    90781 aaggataact atagcaagac tccctcccag aaaaattaga ctgtctcgca gacgtggttg
    90841 cgttgtcttc caatcaaaga gcgcaccaaa cagtgctaat ccaagtggac tggccaccat
    90901 ggtaatcgtt gttatcgttg acataagtcg tccttgtata tgcgttggaa cttctcgctg
    90961 aagccagacc atataaaaag caaaagaaaa cattaatcct taaaaacgga cttatgaaaa
    91021 ataatacatg ccgtttggga tttacaatta gctaccaaat aagaggttat gacaaaagac
    91081 ttttcacaat cccttcttcc gaataaacgg tgttctcctg ccaactcctc ggtcacaagt
    91141 cacaatattt atcaaacatg agaagcaatt tttgtaaatt ccttcttatt tcttgaagct
    91201 gaccacttct gtcacaacct cagaagcgga taaataatat tgtttgatga tactttatct
    91261 ttttcccgtt tgtttataga tcgaatgata ttatagacca agaatcctac ctattaacat
    91321 agctaaaaac acaaaaaagg cactttccaa attgtggaaa gtgccagaaa agttaggatt
    91381 aaggtaactg cgctcagcat tgcttcgccc agtacgattc tcgtaactcg tgaactcgaa
    91441 cgataatcgc aattaacgtt ttgagaactg tgaagcttta cgggctttct taagacctgg
    91501 ttttttcttc gtatcattga ataagtttga aaaaccttga tttataaagg ttttcccgta
    91561 aatatatttt attatatttg attaaatttg aaatgtttgt gttctaaaat gtgttctaat
    91621 ttaaaggcag tatatttcgt attttagatt aaatttttag gctcattcat tctcaaatcc
    91681 attctattca gtcaataaaa attttaaaaa gactttataa acaaagatgt tcaagcgagc
    91741 atcttatttt tttgtgctta atttgaaagg agaacatttc atgtataaaa aacaaaaaaa
    91801 gaaattatgt gtattaacta ttttgagttt ggctgttcaa gcaattgttg gagcaattgc
    91861 tcctacgatt acttttgctg atgaaactac ccatccacaa acggtaaccg tagaactaga
    91921 tttagcacat caatatgctg ttgaaggaac ttttagtgat ggtcgtccaa tgtcagaagt
    91981 cactgttccg cattatgcag tgtacaacgg ctttaaacaa gatattttct gtattgaacc
    92041 aggagtcccg atttacaatg agttcactcc tggctatgag aaaaacccgt taccagatat
    92101 gtccgataaa gccaaattag tttccgtttt atggaaaaaa gtaggtacag atgcagatac
    92161 tcatattgtg gcacagaaga tgatctggca agaagttaat gggtataccc ttcactcaat
    92221 aaaacgatca gatggtagtg ccgtaaatat cgcagccatt gaggcaaaaa taaatcaagc
    92281 cattgacgat tatcagaaga aaccaacctt ccataatagt acggctaaaa cagtattagg
    92341 gcaatcgact actgtgacgg atacgaataa tctgaatttg tcagagttcg atgaggttgt
    92401 ggaaaatgcc gcaaacattg attatcgtgt gaatggtaat cagttgataa tcaccccaaa
    92461 tgccaattca aaagaaagtg gtgtgctgac gcttaagaaa tctgctggta ccggaacacc
    92521 tgtcgcctat aaaaaggctg gccaacagac tttaatggcc ggggcgattg ataaacctaa
    92581 tacctacaca atcaaaattg atgtggaaac ggagggttct ttaaagatca aaaaagtcga
    92641 taaagaatcg ggtgatgttg taccaggaac ggttttccat ttagactttg gaaaaacttt
    92701 acctgcaaaa gacgtgacta ctgacaaaga aggcattgct acattggatg agattcctca
    92761 tggtacaaaa gtaaccatta ccgaaaaatc agtaccggcg ccttatacga ttgataccac
    92821 ccccatgact accaccatta aagcaggcga aaccatttac gtgacttcaa aaaacgcacg
    92881 ggaaaaaggg caaatcattc tggataaaag cggagttgaa acagggagtg atctttggaa
    92941 tgacaattat tccttagctg gtaatacgtt tgccattcgc aaagacagtc caaccggtga
    93001 aatcgttcaa gaaatgacca cggatgaaaa cggtcatgcg gaaacaccaa aagagattgc
    93061 taatgcgttg gaattgggaa cctattatgt gaccgaaact aaagctagtc atgggttcgt
    93121 gaatacgttc aaacctgtaa aagtcgaatt gaagtatgcc aatcaaaccg tagcccctgt
    93181 taccagtaac gtaaaagggc aaaatcaaga agtcactggc gaaaccactt tgacgaaaga
    93241 agacaaagat actggggata aagcacaagg taaggcagtc tttgagggtg ctgaatacac
    93301 cttgtttact gctaaggatg gaaaagctgt taaatggagt gaggctttta aaccagagtt
    93361 agcgaaggga acgaaaggtt ctgatgaaac ggtcactttg actttagacg aaaagaacca
    93421 agttgccgtt aaacatttag ctatcaacga gtattattgg caagaaacaa aagcgcccga
    93481 aggatatagc cttgatgaaa caaagtatcc tgtatccatc aaaaaggtgg atgatagcga
    93541 aaaaaatgcc gtgattaccc gaaatgttac ggcaaaagaa caagtgattc gctttggctt
    93601 tgatttcttt aaatttgctg gatcagctgc gggcactacc gaaactggct tcaatgatct
    93661 gacctttaaa gtgtcgccat tggaagggac caatgaaatc acaggtgccg aagatgaagc
    93721 gaccacagct tataacgagc aattagggtt tgatggttat ggcaagtttg aaaatcttcc
    93781 ttatggggat tatttacttg aagaagtaga agccccagaa ggttttcaaa agattacgcc
    93841 attagaaatc cgttctactt ttaaggaaaa caaagaagac tttgtgaaga gtgagtatgt
    93901 ctttaccatt accgaacaag accaaaaaca accaattaaa acggtgaccg ttccttacga
    93961 gaagctgacg aacaaagcat tttctgttag tttgaaccgt ttaatgctct atgatttgcc
    94021 tgaggaagaa gatagtttaa cttctcttgc gacttggaaa gacggaaaca aagaattaac
    94081 ttctctggat tctaccgagc tagttgataa attgagttac aatctacatg aaatcaaaga
    94141 agactggtat gtggtagcgc aagccattga tgtggatgcg acaaaagctg cccaagaaaa
    94201 agatgaaaaa gccaaaccag tggtgattgc agaaacaagc gcaacgttgg cgaacaaaga
    94261 gaaaacagga acttggaaaa ttcagcataa attaaccgcc gaacaagttt taaataaaac
    94321 tattgtctta ttcaactatg tgtatgaaaa taaggaagct tttgaagcag gcgataagcc
    94381 agtagcgaag gatgttagct tgaataacca agcccaaact gtcagttgta cagtggaaca
    94441 ccatgtttct atccaaacaa aggcccacct agaaaacggt tctcagacct ttactcatgg
    94501 tgatgtggtg gatatgtttg atgatgtgtc gattactcat gatgtactgg atggctcaaa
    94561 agaagctttc gaaacaattc tgtatgcttt attaccggat ggtacaaaca aagaaatttg
    94621 gaaatctggc aagattgatt atgaagtaaa tgataaagaa ttcaccaaaa ctgtgcttgc
    94681 gaaaaaagta gataccggaa aatatccaga aggaaccaag tttacttttg cggaaatcaa
    94741 ttacgataaa gatgggaata tcaatggaaa acacaatgaa gatttgaaag aaaaatctca
    94801 aaccttaaca ccaaaagaag ttccaaccat actaagtacg ccgaaacaac cggaaacacc
    94861 aactgttcca agtggctctc aagaatctag tcccacagtg aagaccttcc ctcaaaccgg
    94921 ggagaaaaat tccaatgttt tactgttcat tggttttact ttgatttttg cgacggcggg
    94981 ctattatttc tggaatcgcc ggaactaagg tgatgagatg atacaagtaa atcctaagaa
    95041 ccaacgtgcg cgaccgcctc ctaaaaaagt agtcgcgcta tttttgtatc caaaactaac
    95101 aatgaaaaga ggaaaagaag atggaattga aatttgtcgt gccagatatg gctgaaacct
    95161 ttggaaagtt aagttatgcc ggtgaaggag aaattataac agaaggatat ggacggaata
    95221 ctacggtgat tggtcgcagt tatcatttgt attccagtaa gcaacgggcc gatgatattg
    95281 aggtggtagt agctgcggaa gccggtgaaa aggattttga tcaagaccaa ccgctaaaag
    95341 ccgtgaatcc ccatttggtt gccaaaggct atgagattga gaatcgtggg tttactgatt
    95401 acgtgttgta tgttgatgat ttagtgaaag cataggagga aaagaagatg agattaacag
    95461 aaggtattgt cgtagattca ggtttaacat ttgggaagtt gcgtttttcc gcattacgcc
    95521 gagaagtacg gaagcaaaat gaggatggaa caattggtaa tgaggtaaaa gaacgaacct
    95581 ataatttaaa atcctctgca caagggcgaa tgattcaagt tagtatccca gctaatgtac
    95641 ccttacgcga gtttgcttat gatgcagagg tagagttggt caatccaatc gtggataccg
    95701 ttgccaatta tgtttttcga gaaggaacta ccgttaattg gttcattaag gcggatgatt
    95761 tagtcttgaa gcggcagccg aatcaaggaa atcctacgaa ccaaaatgaa agaaagaaat
    95821 aggtgaagat tatggaaatt aatgttgaac atattttaga ttgtctcgat caatatggaa
    95881 aaggcgagct gactgaggaa caactcgtta aagcattaac ctctgatgaa aaaatgtttc
    95941 tgattataca tcaaggttta cttggagaaa atagcgacag tgaagaagac tctgatgtac
    96001 tagactggtt aggagaagaa gagtctttct ttatagttgt cgaagtaaat gaaaacttat
    96061 gtcggcaggc agaatccatt ttggaagaaa ttggtgtgga gatgccggat gctattgaag
    96121 gctttttaaa tcaattggtt gaaacgaaac aactcccctc ctcgtagcag taaatgacta
    96181 gcataacttt ttgaaattgc ctaatgaaat cctattgggc tttttcgatt ggaggtggaa
    96241 accatcaaaa tgtacaaagg acatcgcata cgcgcaggag atcagcactt ggtttatcac
    96301 tttgttcttg gctggctact cgctctgttc atcggttgga tgagcgtctt ttattttcaa
    96361 gagtttagac aatttgatat ttctaagcta tcactttcta ctattgaaat cgtttggtct
    96421 ataaaagatt tagtatgtct gcttggaagt ctcgcttttt caggagctat gatattgctt
    96481 tatattcact tttttcttga tcattggcga agcttgtggc atcgacaaaa gctagctcgg
    96541 atgattctag agaaccattg gtatgaagtg aaacaaactc aaagtgaagg ctttttcaaa
    96601 gatttgaata gtagccgaac cagagagact atcagttact tccccaaaat ctgttatcga
    96661 atgaaagatg gattactgtc tattcgcgtt caaatttcac ttgggaaata ccaagatcag
    96721 cttttaaaat tggaaaagaa gttagaaagc ggactgtatt gtgagttagt agaaaaggaa
    96781 ctcaaagact cctatgtgga gtatacttta ctgtatgata tgatcgccaa tcgaattggg
    96841 atagacgaag tagtggcaga aaatgggact ctgcgattga tgaaaaatca agtatgggct
    96901 tatgattctt taccccatat gttaatcgct ggtggcacgg gtggagggaa gacctatttc
    96961 ctcctcacca ttatcgaagc actattgaag tcagatgcag aattgtttat cctcgatccc
    97021 aaaaatgcag atttagctga tttaggtacg gtgatgcctc atgtttattc acaaaaggaa
    97081 gaaatttctg cttgtgtgga agatttctat gaacgtatga tggctcgtag caaggcaatg
    97141 aaagaaatgt ctaactacaa aacaggagag aattatgcct atcttggact tccacccaac
    97201 tttttaatct ttgatgaata cgtggcttat atggagatgt taacgacgaa agaaagtgcg
    97261 gtgattttga ataagctcaa acaaatcgtg atgttgggtc gtcaatctgg tttctttctg
    97321 attttggctt gccaaaggcc ggatgcgaaa tatttaggag acggaatccg cgatcaattc
    97381 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
    97441 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
    97501 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
    97561 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
    97621 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
    97681 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
    97741 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
    97801 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
    97861 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
    97921 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
    97981 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
    98041 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
    98101 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
    98161 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
    98221 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
    98281 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
    98341 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
    98401 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
    98461 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
    98521 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
    98581 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
    98641 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
    98701 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
    98761 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
    98821 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
    98881 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
    98941 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
    99001 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
    99061 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
    99121 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
    99181 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
    99241 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
    99301 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
    99361 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
    99421 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
    99481 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
    99541 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
    99601 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
    99661 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
    99721 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
    99781 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
    99841 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
    99901 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
    99961 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
   100021 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
   100081 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
   100141 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
   100201 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
   100261 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
   100321 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
   100381 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
   100441 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
   100501 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
   100561 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
   100621 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
   100681 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
   100741 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
   100801 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
   100861 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
   100921 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
   100981 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
   101041 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
   101101 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
   101161 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
   101221 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
   101281 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
   101341 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
   101401 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
   101461 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
   101521 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
   101581 gaatttcgga caaaatttat gtgcgactcg ttccttagtg aaaagctaag gcactaataa
   101641 aagaacgaaa aagtttggtt attaggagaa ctgataatct gacaggtgga atgatgaagt
   101701 aacgctctga aacgcctgcc ctgagcctcc gacaagcatt gtatgaggaa actcagaatg
   101761 tttgaagctc ggtaaagacg gctgaaagat accgtaatag ttgatgaaca tatatcttct
   101821 atcgaaaact atccagaggt gggtagcgta tccaacaggt cgggggtcta taaaatatct
   101881 atggttagaa tgttctataa ggaactgacg aaacagcgaa tgtacgggtc taaatttgaa
   101941 ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg tggcaaagta agaatatagg
   102001 ttatgaaatg ccatataacg ttacaggcgg tatcaagcca gcaggcttat agctagtgac
   102061 ctaaggatat atatgtatag ataagattat cggaacgagg aaaggtattg ggttctcatc
   102121 aagagaataa actgacgaag aacaataagc agttgaacta atgctgaaaa gcagagggcg
   102181 aacctaagaa attcttgtaa tgagaatgga ggaatgccct caagtcgtgt atatattaga
   102241 aaagtattat gttgtttcaa taaggattac gagtaagata aaaaagctca ctcaatttca
   102301 agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa acattgtgtg tagaagattt
   102361 aagacacgct gaatattacg aaatgcaaaa cacctttgat gatttatatg ccaaaagtaa
   102421 gaatggagat attttcactc atcttatgga tattatccta tcgagagaaa atatattact
   102481 ggcatatcga aatatcaaag caaatgcagg cagtaaaaca gcaggaacag acggtacaat
   102541 aattaaagat attggcaaat tgccagcaga aacagttgtc aaaaaagtaa gatacattgt
   102601 tgcaggcagt ccacatgggt atcgacctaa gccagtgagg cgaaaagaaa taccaaaacc
   102661 aaatggtaaa accagacctc taggtattcc ttgtatgtgg gacaggctta tacaacaatg
   102721 tataaaacaa gtattagaac caatctgcga agctaaattt agtgaaaaca gctatggttt
   102781 tagacctaat aggtcagttg aaaatgcaat aaaagcaacc tataaccgcc tacaaatcag
   102841 ccagctacat tatgtgattg aatttgatat taagggtttc tttgacaacg tgaaccattc
   102901 caagctgata aaacagatat gggctatggg aatacgggac aagcacttga tattcatttt
   102961 aaaaaggata ttaaaagctc caatcaaaat gactaacggt acaataacat atccagagaa
   103021 aggaactccg caagggggaa taatatcccc actactagca aacattgttc ttaatgagtt
   103081 agaccattgg gtagaaagcc aatggcagga aaatcctgtt acaaaaaact atgtggttca
   103141 tatcaataaa agcggtagcc catgtaaaag caacgcttac aaagaaatga agaaaacaaa
   103201 gttaaaggaa atgtatatgg ttcgttatgc agatgatttt agagttttct gccgctacaa
   103261 ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg attgaacaac gattaaaatt
   103321 ggaagtatca caagaaaaaa caagaattgt aaatgttaga aaaagatact ccgactttct
   103381 tggtttcaag attaaaatga taccgagaag aaagaagtta gttgttaaat cacatataag
   103441 tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca caggcaaaga aaattgcttc
   103501 tccctcaaaa gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg
   103561 tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag
   103621 agctgtaatg gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa
   103681 aacaggcaga caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg
   103741 gtatgttgct ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc
   103801 aatggcaaag gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac
   103861 caatctaagt gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg
   103921 cacaatagaa tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc
   103981 tataactctg gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac
   104041 tggagctggt ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg
   104101 tctaatacac gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa
   104161 taaagtccag ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa
   104221 cttgatatag gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc
   104281 ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag
   104341 gaacttacct atccatatat caatggtttt taaccaatgc ccaatcctta gtcttactgg
   104401 caattgtggt cattggtttg tttttaggat ttaagcggga gttttccaaa ctgattggct
   104461 ttttggtgat tgctttgatt gcggtcggat tggtctttaa tgcttctggg gtgaaggatg
   104521 tcttactcag tttgtttaac cgaattgtcg gtgcgtaagg gggaagcacg atggaacaga
   104581 tgcgtgttta tattgcgaac ctcgggaaat ataacgaagg tgaattggtg ggggcgtggt
   104641 ttaccccacc tgtcgatttt gatgaagtga aagaacgaat tgggttaaat gacgaatacg
   104701 aggaatatgc aattcatgat tatgaacttc cctttgagat tgatgagtat acgccaattg
   104761 aggaaattaa tcggttgtgt ggcttagcgg aggaacttga aggaacgcca gttggtgaag
   104821 ttgcttctga gattcaacat gctttcttca attcttttga ggagatggta gagcatgtgg
   104881 atgatattat ctattatcca gattgcgatg atatgagtga tgtagcatat ttcttgattg
   104941 tagaagctgg tgattttgga gaagtaccag aacacttgaa gacgtatatt gattttgatg
   105001 cgtgtggtag agacttggag attggaggca attatttggt aacgagtcga ggtgtttttg
   105061 agtatccata ttaacaaaat tattgaaggg tagttgattt ctaaactatc cttcttttta
   105121 aaggaatgat ttgatgaaga aaataaaaag ctacaccagt atttgggcag ttgaaaaggt
   105181 catttatgca attaatgact ttcaactgcc ttttcctgtg acttttaacc agatggcttg
   105241 gtttgtttta tccttgctgt ttgtcatcgt atttgcccat gtgcctccac tatctatgat
   105301 agagggggcc tttttaaaat acttaggtat accggtggca gttacttggt tcatgtcgca
   105361 aaaaacgttt gacggtaaga aaccattggg atttttacga tcttttatta gctatcactt
   105421 acgatttaag gtcactttcg caggaaagaa agtcaaagaa cagaagaaac gatgggacga
   105481 acccattact ctggtaagga gtgtgaacta tgtacccgat taagtatatt gaaaataatc
   105541 ttgtctttaa tcaagaggga gaatgttttg cctactatga gttaatacct tacaattact
   105601 cctttttgtc acctgaacag aaatatcaag tccacgataa ctttcgtcaa ttgattgcgc
   105661 aaaatcgtga aggcaagatt catgccttac aaattgccac agaaagtagt attcgtgcca
   105721 cgcaagaacg atcaaaaaag gaaattaccg ggcgattaac agaagtagcg aaacaacgaa
   105781 ttgatttaca gacagaggca ttagtatcca tgattggcga tagtcagatt gattatcgct
   105841 tttttattgg cttcaaactg attgcgacag atgaagaggt caatctaaag aatctcaaga
   105901 aatctttctt ctccggcttt caagaatttg tctatggagt gaatcatcat ttgatgggcg
   105961 attttgtttc cttatccaat gaagaaattc gccgatatac caagcttgaa aaactaatgg
   106021 aaagtaaatt ggctcgaaga tttaaagtca gacgagtcac accgagtgat ctgatgtatt
   106081 tgattgaaca tatctatggc gaaaagggta caccatttga agaatatgaa tttcagctac
   106141 caaagaaaaa gctgaagtcg gaaactttgg tgaaacgtta cgacttatta cgtcccagtc
   106201 gttgtttgat tgaagaaaag ccccggtact tatgcatgga acatgagaat catgaatcat
   106261 atgtagcgta cttaacaatt aatacaattg tgggggaaat ggagtttccc tcctcagaac
   106321 ttttctatta ccagcaacaa caatttacat tcccaattga cactagtatg aatgtggaga
   106381 ttgtgacgaa caaaaaggca cttgctaccg ttcgcaataa gaaaaaggaa ttaaaagact
   106441 tggacaacca tgcctatcaa tctgacaatg aaacaaattc caatgtctta gatgcattgg
   106501 attccgtaga tgaattggaa actaccttgg atcagtccaa agaatccatg tacaaactga
   106561 gctatgtggt tcgagtaagt gccgaatcag tagatgaact aaagcgccgg tgtgatgaag
   106621 tgctagattt ttatgatgat accaatgtaa aactagtccg gccttttggc gatatgatgg
   106681 ggctacatga agaattctta ccttctagta agcgatacat gaatgattat atccagtatg
   106741 ttacgtctga ttttctcgcc ggacttggtt ttggcgccac tcagatgtta ggggagttgg
   106801 aaggcattta ctttggttat aacgtagata cggggcgcaa tgtttacctg aaaccggcat
   106861 tggcctcaca aggggtcaag gattcagtta ccaatgcgct agcagcggca ttcctcggtt
   106921 cactcggtgg cggaaagtcc tttagtaaca atctcttagt ttattacgcc gtcctttttg
   106981 gtggacaagc tgtgattgtc gatccaaaag gggaacgggg tggctggaaa gaaaccttgc
   107041 cagaaattgc ggatgaaatc aatattctca acttaactag taagccaaaa aatcaaggat
   107101 tacttgatcc ttatgtgatt atggagaaaa agaaggattc tgaaagtcta gcaattgata
   107161 ttctgacctt tttaacagga atttctagtc gagatgggga gaagtttcca gtgttgcgta
   107221 aggccattcg gacggttacc caaagcaatc aacggggatt attacaagta attaatgagc
   107281 ttcgaaaaga aggctcacca gttgcggaaa atatcgcgga tcacatcgag tcctttaccg
   107341 attatgattt tgcccatttg cttttctcag atggttcgat tacccagtcg attagtttag
   107401 ataggcagtt aaatatcatt caagtagcgg atttggtttt accagatgca gaaaccactt
   107461 ttgaagaata taccaccatg gaattgctta gtgtttccat gctaatcgtc atttccactt
   107521 ttgccttgga ctttatccat agtaatcgag aaatcttcaa aattgtagac ttagatgagg
   107581 cgtggagttt tcttcaagta gcgcaaggga aaacattatc gaataagctc gttcgtgctg
   107641 ggcgggctat gaatgccggg gtttactttg tcacgcaaaa tagtgatgat ttactggatg
   107701 aacgcttgaa gaataatatc gggttgaagt ttgcgtttcg cagtaccgat attcatgaaa
   107761 tcaagaaaac cttggaattc tttggcttgg atcgagaaga tgaaagcaat caaaaacgcc
   107821 tacgagattt ggaaaatggc caatgcttga tgcaggattt gtatggtcgg gtaggcgtgg
   107881 ttcaggtaca tccagtcttt gaagaactct tccatgcctt tgatacccgt ccacctgttc
   107941 aagagagaat ggagtgaggg gatgaaaaag aaactttttc ggattgtggg aatcgttttt
   108001 attagcatac tcactctgat agttctattg agtttagttg gtacggtagc cgaggcgaca
   108061 gggctagttg atgatacagt tgaagcagga aatctatatt ccaaatattc actgaacaac
   108121 tatcaactag atttctttgt ggatagctct tgggattggt tgccttggaa ttggggtgat
   108181 ggcttaggga aaagcgtgat gtatggtctt tatgccatta ctaatttcat ttggacggtg
   108241 agtttgtatt tgtcgaacgc cacgggttat gtggttcaag aggcttacaa attagatttc
   108301 atttcggata cggctgaaag tattggaaag aatatccaaa cattggctgg tattactgaa
   108361 aatggactcc agacctccgg attttatttt gggtttctat tattgatgat tttagctctg
   108421 ggggtgtatg tggcgtatac cggattatta aaacgagaaa ccacgaaagc agttcgagcc
   108481 gtgattaatt ttgtgatgat ttttctacta tcgggttcgt tcattgctta tgcgcctacc
   108541 tatatcacaa aaatcaatga ctttagctca gatgttagtg aagcggccct tacccttgga
   108601 accgagatcg ttgtacccaa ttcagaaagc caaggaaaag atagtgtgga tttgattcgg
   108661 gatagtttgt tttcgattca agtccaacaa ccttggttac tactgcaatt tgatgattcc
   108721 aacatagaag aaattggtga agaccgggtc aacaagattc tatctgtgag tccagatgaa
   108781 aacaaaggca aagatcggga agaagcggtc aaggcagaga ttgaagacaa tgacaatgcg
   108841 aatctcagta ttaccaagac catgaaccgt ttagggaccg tggtcttttt ggtattgttc
   108901 aatattggca tttccttctt tgtttttctt ctaacaggga ttatgttgtt ctcgcaaatt
   108961 ctcttcatta tctttgcgat gtttctgcca attagtttct tattgtccat gttgccgact
   109021 tatgaaagtt taggaaagaa ggcgattatt cgtttattca atacgattat gatgcgagcg
   109081 ggtgttactt tggtgattac gactgcattt agcatctcca cgatgttttt caatatttcg
   109141 gcaacctatc catttttcat ggttgctttt ctgcaaattg tgacctttgc cggtatctat
   109201 ttcaaattgg gcgacattat gagtatgttc aacctccaga gtaatgatag ccagtcaatg
   109261 ggaagacgcg tcatgcgaaa accgcaaatg ttaatgaatc gaaaattgcg gcaactcaat
   109321 cgaaatgtgg gacgagcttt ggcttttggt ggtggtgcag cagtcggaaa taagttggca
   109381 aaggaacaat caaaatccaa atttaagcca tccggttctt ctctacgaaa gaattcacga
   109441 ttaccgaacg atcacgaaat actatctgac ttaactaagg agaatccagt ttcaaataat
   109501 aagaaacaat cccgaatgaa tttggctggg agaaaaatcg gcaaagtttt ggatacccaa
   109561 gctttggtga aagataagac taaacaagta aaggatcagg taaggaatac gccaacgaat
   109621 ttgaaataca atctccataa aggaatcgaa aagtcgaaaa aagcaccaga agaattcaag
   109681 cgcggactgt tccaagaaaa agcggatcga gtggatttgc gagaaaaaca acgacaacga
   109741 agaaatgcga aaatggacga aaaacgcaag atactgagtg aagcaacaaa tcgtcaggga
   109801 aaaagaaggg cgaatattcc aatagataaa gcagctcaac ctcagaaaga tatgatgctg
   109861 aaaaaagaag caccaaagcg gattgtgaga gcaaatctga atcccgaaat caaacgaaaa
   109921 ctaactagtc aagaaatcat ttcaaaaggg aaaagccaat cagcaagtaa gaaaaattct
   109981 ttccaacaag tgaaacagag gtccactctg ccaaagcaga ccaatcaaaa agtacaaaaa
   110041 agaatgaacc atcgtccaaa atcaaggtca ggtgagaaaa aatgaacttg aagaaagtgg
   110101 gtgttatttc attaactttt ctgaccatct cttttatggg tattttgatg tgtgtgggtt
   110161 tgttgtttgg tgaagacagt gagggaaatg gtagttctgg cacttctcct ggcggaacta
   110221 gtgtctcaga agaagtattg aaacaccgtg caatggtgga aaagtatgcc aaagaatcgg
   110281 atataacaga gtatattcca attcttttgg cgattattca agtggaatct ggtggcacaa
   110341 tggaagacgt tatgcaaagt tctgagtcag ccggattacc acctaattca ttaagtaccg
   110401 aggcatccat caaacaaggc tgtctctatt ttgcgtcttt ggttaagcgt tccaaagaac
   110461 tagggtgtga tcaagatagt attattcagg cctataacta tggtggcggg tatctagatc
   110521 atgtcgggaa aaacgggaag aagcatagtt ttgctttagc tgaatctttt tcaaaggaaa
   110581 aatcaggtgg tcaaaaagtc gattacccca acccaatcgc gattaaggaa aatggtgggt
   110641 ggcggtataa ttatggcaat atgttttact gtttattagt gaagcaatac ctaactacaa
   110701 ctcagtttga tgacaaaata gtccaagaaa tttttgatga ggcatttaag tatgaaggaa
   110761 cggcctatgt gtttggtggc tcaagtccag aaacggggtt tgattgtagt ggcttgaccc
   110821 aatggtgtta tgccaaagtt gacctgaaac ttcctcgggt ggctcaggat caatatgatg
   110881 ccatgactca tattgatcta aaagatgcca aaccaggtga tttagttttc tttcattcaa
   110941 cctatgatgc cggtacttat gtgacccatg tgggcattta cgcaggggag aatcgcatgt
   111001 ttcatgcagg agatccagtg gggtggacga atctaacgga aaactattgg cggcagcatc
   111061 ttatcggagc tggccgatac aaataagaga aaagaggaaa aagaatgaag ataaaaattg
   111121 aacggaatca aaaggaaaag agcccaaaaa agaaaaaaga acgagttgta tccgtgggta
   111181 agcatcgaaa aatggtgctt gccctttggc ttttattgtc ttgtagtctt gcttttggcg
   111241 tttataaaaa tttcaccgcc attgatcaac atacaactca tgaaaaagtg gttataaaag
   111301 agaaggtagt gaatacttct ggcgttgaaa gttttacaga ggattttgcg aaagaatatt
   111361 tttcatggaa aaataacaaa gaagcaattg aaaagagaat gagtaatctc gaacagtacc
   111421 ttacagaaaa cggtctcgct ttaatccaag atatggttcg agcagatatt cccacgagtt
   111481 ccgaagttca atctgtaaaa attctagatg ttgaaaaaca ctcggaagaa tttgtcgttt
   111541 catttttagt ggagcaacat attacagagg gaaaaaagac gcaatcaatt tcttctgcat
   111601 atcgagtgac aatctttgaa gatgaaaaca ggaatcattt agtaactagt ttgccaacaa
   111661 tgattggaaa gccagacaag gcaaaatatg aagcaaaaca agtagaaaac gattcagaaa
   111721 ttgatgccaa aacaacggaa gaaatcaccg attttttaga aacatttttt aaactttatc
   111781 catcagcttc tgaaaaagaa ttggagtatt atgttgaaaa tggtgcgatg cgaccaatca
   111841 atattgactt aaagttttcg gagttagaga atccagttta tctacaaaac ggaaaaaatt
   111901 ttctagtgag tacaaagata aaataccttg ataatgtatc aaaaaccact agctactttc
   111961 agtacaaatt agtattacaa aaaaatggaa actggaaaat cgttgatgtt aaataataat
   112021 gactcaatct aattttatta gaagttataa gtagtgtaac ttatttcagg cagaagtcgc
   112081 ccatcctcta taggtgggtg atgaatgcca ttcggtaact cgaaaaccga caattaagac
   112141 atagtgcctt tttattttaa tccatgtcat aatcagtcta tataggaaat ggttgatata
   112201 ccatggaatt agatacaaat aatcattcag tttttcttct tggttacccc cttattttag
   112261 tagtgaaaca ttgtaaacat gtcattgatg atgtaatgtc agcttatgca aaaactgcct
   112321 ttgaaagaat ttcagaatca catcacatta ccttagatga atgaaatcgt gataaagaat
   112381 actttcatat tatgctcaaa gcacaaccta aaaccgaatt aacaaaattc agcaatgcct
   112441 aagctcacgt ctgataaaat gagactttct aagggtcaaa aatttccttt gaatacgatt
   112501 attgcttaga taaaagtaaa agccttagaa ctacggggat agcttggtta ataagagata
   112561 tcgttgttag taaagacata ggcttgcaag tatgctctat tcccaagaat ctcccccttc
   112621 aagcaccaag gatcgttaaa ttcagctaag tggcaagtaa ttcactagac gataattatc
   112681 tccatatttc aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat
   112741 ctaaaagatt cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta
   112801 aattgatacc agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta
   112861 ttaaattcat atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta
   112921 ctttgaaaat ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat
   112981 cttattataa ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt
   113041 aagcctctca tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctaaa
   113101 cagcggtatc tcttattaac caagctatcc ccgttgttcc taaggttctt gatgttttct
   113161 aaggcaatgc ttgtattcat agcccttggt caaaatattg atactatcgt tgacgtttcg
   113221 attatgatgt atacggcaaa taggacaagt ccattctcga attgtgagtg acttcttacc
   113281 atctgtgtgt ctacattctg aaccaatttg actagacgga aatcatttat ctatcttaat
   113341 gattttttga cagaagtatc gaaaatcttt acataatctg ggtgatgtat tcttatgcag
   113401 atgagagcgt tggctccgta ttcataacca gtagcctcta aaacgtagtc catcggtggg
   113461 atacctagct tgcggttgcc agcggctttc gcagtcttct tgtgatcaaa tcaagcatct
   113521 cattgagcac tagtattttg aagataatac ttgagttttt cggcttttag aagttcactt
   113581 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
   113641 acagtggttt tttcagacat ttttctgaat acctccttgt tttttgatac gccagatagt
   113701 caggaaaaag gcaacaaaac acaaaacaaa gctgcctaaa aaggtaatct tcattccata
   113761 taaaaagaca tccggccgat ttgcgatata gttagttact tgctggccca tcttagcaga
   113821 catcgcttga tataagaccg tcgtagaaag cgaaatcccg ctgaccatcc ccaaatttcg
   113881 ggcaaaggaa ttcatcgagc ctgccagtcc caagttttcg atagtgaccg agctcataac
   113941 catcgtatta ttgggggact ggaacaaggc attgccggca cccatgatgg cagtagcgac
   114001 aatgtatagc cacaaaggag agtccactga caaaaacata tacatgacct gggttaccga
   114061 caataagccc aagcctacta ataccaaaat ctccgggcca acccggtcgg tcaggtagcc
   114121 gcttaaaggc gccccgacga ccatcatcaa aggaaagacc atcatcaaca gcccagcata
   114181 gctaggactc aacccgcgag cattttgcag ataaaaagga atcactacat tgacaaagaa
   114241 gttggaagaa aaaatcaaca aggctgtaat caagctcatg gtaaaaatct tgttcttaaa
   114301 aataccgaaa gaaatcaatg gagaggctgc tttcttttca acttttaaaa agctgacaaa
   114361 ggcaatcact gaaattacga acagtacaag ggggactgga tggaaaaagc cactttcttg
   114421 ccccaaaaag attgctccaa aaaagctgat aatcgtagcc gcaatggcag caaagccccc
   114481 tccatcgatt gtatggccgc tcatggtgat atcttttggt aagaattttt cagcaatcaa
   114541 catcgttacg attcccaccg gtacattgat ccaaaaaatg tacgcccaag aaaattggga
   114601 caaaatcaag ccgccaagac ctggtccagc aatcgagcct aatgaaacaa acgcacctat
   114661 tgctcccaat gcccgaccac gttcatgtat gggaaagact tccgtgatga ttccggcatt
   114721 tgtagccata gtcatactag caccaatggc ttgtaccacc cgtgcgaaca ataaaatacc
   114781 tagcgaatga ttgaacccac acaacagcga tcccaagaca aagatcagcg taccaatccg
   114841 aaatactttg attttcccac ggctatcgcc aattttacca aacagcaaca aacaagaaca
   114901 gacaatcatc agataaacgg acactaccca ttccgcttga ttcattggta tgcccatatc
   114961 atctgagatg gtaggtagtg caatattgac aatactgcca tccaatgtgg acatgaaggt
   115021 aaacatagat atagcaatca aaatccacca gcgatttttt tggacttttg catcttcttg
   115081 ataactatac ataataggat ttcctttcta atgatcgtgt agctaagaac aatataacct
   115141 atgtctactg cccaacaact tctcgcgact ctagccatag gaagcttgag taatttcgat
   115201 ttgatatcct catgtaacca tgtaagttgg agatgcttta tttgaactta ctctatagta
   115261 actagtgata attcgcttta ctttcttcta gcaatttcta aaagtcgact tcattgttct
   115321 tcagattaat agtgtgagct atctcgtgag acgtaagcaa atttccattc tactttttcc
   115381 caattttttc atatttatgg tgttgcacct atttgttttc caatttgctt ttcaaataca
   115441 ttgctatgat attcaattcc ataggattat actaaatatc aatagtatga ataatatatt
   115501 atagatgttt atatatcact gttaattatt atccacaata attaatgatt acctcgatat
   115561 ttgtcaatca aaggttgaaa caatttattt tcagcatctt ttctagcttg agctgcactc
   115621 ttaatagaat catacgatcc taaatagtac ctcttttttt ttaaagttat atacgcaatc
   115681 tatttctctt tagaacgata aactccctta acaccacttg tattattttt cggtaatttt
   115741 gacgttagac actctattac tgtcccttca acacatgtcc ttgtttcttg cctttttaaa
   115801 catccacaac tttgagtatg tccaaaaaat aagttactat aacttatctc acacatactg
   115861 ccacactcac aatgacaaac ccattttgtt ttccatgaac agttattgtc caaaggatac
   115921 tttgcagtaa gtaatccgaa ttttttattt tcaatgtgat gcttttttga tttcaaacat
   115981 ccacagcttt ttacatcacc ttttagaagt cgctttgccg atactaactt ttttctccca
   116041 cacagacatt gacattccca aacaacagtt ccttcacttc gcagtttagt tggcttgatt
   116101 gcaactaact taccaaaagt tttatttgtc aaatttttat atttatgcct atttttataa
   116161 tttccacatc cacaactttt ggttctacct gatagtaagt ttgatcgata tacaatcgtt
   116221 tcattcccac agtcacatag gcaacgatat tttttatatc cattatgagt aatctcctca
   116281 acaacagtaa ggtgaccata tctttccttc actgcaccta ccctcccaag aaagaagtac
   116341 catataattt caattaactt ttaataaatc agaaaaaaat atttccaatt tttcttagtc
   116401 tgattttatg actaatttac aatacaagac tgaacgatac gtacacgaaa tcctcaagat
   116461 agataaattg ttttcatcta gtgaaacctg ctcaaaatat gagcataaag acggcaataa
   116521 tacactcgat attcgataat ggacttgttt tatttgtcaa acccatcgta atcgagatat
   116581 caattccagt ctcaatatcc tgatcaaagg tctaagaatc caaccatttt cttgttactt
   116641 tgtcagattg attacctttt ttagtctccg aataataagg ttttcggatc aactaacatg
   116701 ctctgagaaa gactactatc tttgcaaatt tccaaatgaa ggtgtgggcc agtactattc
   116761 ccagtacttc ctacatagcc aattatttgt ccttgcaaaa cattatctcc agtttttact
   116821 aggtattgtt gctggtgagc atataacgcg gttgatccat cttcgtgttg tatcaccacg
   116881 taatttcccc aagagtagtg aaactctgct ttaacaacct taccagcttt aactgcatga
   116941 atcggctcac cctgtgtagc tgccatatca agtcctcggt gaaattcatt tccacgattt
   117001 ccaaacaaac tggttattga ataattatga ataggcactt ggtatccagt cttactcatt
   117061 tgtggacctt taacttcttt atcatagttg gttaaatcgt aggttttaat taattcattt
   117121 agcttcaaat agtattgtgt atctgttgca taccgtcctg taaggaattt agttgcttct
   117181 cgatatgttt tactattagc ttttaatgct cctgcataaa aatctttatt tccactaatt
   117241 cccttagtta ataatttggc ataatcttca aagctttcct catagttttc atacactcta
   117301 aattttgatt gggtcgtata aagcgttcca ttacccaaat cttcgtttgt taccataatg
   117361 acaaagtttc cattataagt tccttttatc ccaaataaat tgtaattcgg tgcctgagct
   117421 aactggcttt gaccactggc agattctaag atcgcttgtg caatcataac agaagcatat
   117481 aaatcatatt tctgaccaat ttttctcgca gactcaccaa ttttcataat aaaattttct
   117541 acagattcat ttttgtcaaa atgaatttct tcattaccag atagtattgg ttgtgattgg
   117601 aaagattctg ttgattctat ttgctgtggt gtcgaaaaag tttttgtttg tccttccgtt
   117661 tttggcgctt ccttttttga agattctata tggtttggtt ttgtcacttt cgaagaagtt
   117721 atttcacttg tactacttga agttgttgaa gattcggtac ttgaaatcgt actactttca
   117781 cttgacgaag ttgttgaaga ctcggcattt gaagtcgtac tactttcact tgatgaagtt
   117841 gttgaagact cggcatttga agtcgtacta ctttcacttg atgaagttgt tgaagactcg
   117901 gcatttgaag tcgtactact ttcacttgat gaagttgtca aaagttcact actctctgct
   117961 gttacatcgg aattactcat ttcagtcgtc tcggttcttt cttttgcaat tttgtatgtt
   118021 gaattttcgc ttgccaacac atggaaaggt gttgctataa ggagttggga cattaatata
   118081 actgctgatg caagttttaa agattttttc atttgattta cctctctttt ttctctttaa
   118141 tagtgttttc tattaaaaat tatgataaat attttcaatg aattgagtaa tacagtatat
   118201 ttcttgattt catttagtaa cgattatgtt tcaatgttta atcctgttac tgcctttaaa
   118261 ataaaattat agggttggct aaaactagga tgaaataaat aatctaacat tttcaatttg
   118321 tctaatgtaa tttgttcttc tatagcaaga gaaaacattg ataatagtcc agataaatct
   118381 tcttgactac aaagttgtac acctactatt ctctttgtat ttttttcaaa gctaattctt
   118441 aattttacac gactattttt tctcaaaaaa gcaggtctaa tgaaatcttc tatttctata
   118501 taatctatag actcacttaa taatcgtgct gtttttaaat tcaaacctgc tgcgtaaaaa
   118561 ttgtaattaa atatagaaaa gccatttgta aattgggaac cagaaggtgt aaaatcttga
   118621 ttgttaatat gttgagttgc aatataagct gttcttagag cattagaaat agaaaaatca
   118681 acactacttc tattaatgga cgtaaaaaaa gttgtagcac aatccccaat agcatatata
   118741 tcttttatat ttgtttgctg gtattcatta gttagatatg ctccactaac atctctcaag
   118801 agttcctttt ccccaattgg actattagat agaaatccta tagcttgtac gaccatatcc
   118861 atagagtatt gggtaccact tgtagtgact attttttcaa tcttttttct tcctaaatat
   118921 tttttaggac tttctgatag acaaagatgt attccatttt ttgatagtat ttcttcaact
   118981 ttatctgaaa aactacggtc gtaataagtc gacaacactc tatctgcaac atcgaaaaga
   119041 tatacttctt tccctctttt tcttatggct tcagcaattt caactccaat atatcctgct
   119101 ccaataattc ctactttttt aatagtacta tcatccaaag ctttaaaaat ctcttttgca
   119161 tttggaaaat tctttatcga aaaaatatta gctaaattat tccccggtat atctagcata
   119221 atttgctttg aacctgtagc taatataact ttgtcatatt cttgagagaa tcttgtacca
   119281 tcttttttta cacaactaat ctttttattt tgaatatcaa ttttgtcaac ttctgtctct
   119341 agaaataact tttttatctc agaatttagt gtcattctat tttgataaaa aagatcttct
   119401 aaattactta cttctccttt tataaataac gatgtcccac attccaagta actaatttct
   119461 gattttttat cgattagaat aacttcataa tttgaatcgc ttaagtttag aaatcgagct
   119521 gcggctagcc ctgaatggtt tgaaccaata attactattt tttccactta attacattct
   119581 tttcctttct ataaaaagta tattcatctt taactaacac ttatttccca tcataaaaaa
   119641 gcaaaaagag gaagaaaaag attcttcctc ttctcagaga agacttaaga aattgatttt
   119701 ctattcattc tctttttttc gatttttata ccctaataat cctaacgttc ctaacagtat
   119761 taaaccaccg ctaatagtaa agatattact gtcactctct cccgtcttag gaaaaacttt
   119821 tgaggactgg cttttgcctc tatttttgtc ttgattgctg ttgctacctt gattgtcagt
   119881 cttactacct ttactaccgt tttttccact gtcttttttg ttggaagaaa caactacatt
   119941 agtaggttca ctagtattac cagattcatc agttgcagtt actatcactt ttgtccctgc
   120001 ctctaattta ggaactttaa ctgtaaaatt accattttta tcagctgtcc catggtgttg
   120061 acttccgtct ggtaatgtaa ctgtcacctc tgcatttggt tcagttttac cagtaacaat
   120121 ttggtcaccc tctgtaatag gatcaacttg aggtttattc ggtgcttttt tatctactac
   120181 agaaatagta acttctactt cttctttaga tccatctgga tatgttacta ctacagtacc
   120241 ttttttgttt ccagctgtac taacatctac tttttctttc caagtataag tagttccttt
   120301 tggtagatct tctttgttct taattccctc agatgcatct ggctctttat ttaagtctgt
   120361 agtcactttt tgtccttcag gattatattt atctgcatcg gaacgagtat ctgtcactgt
   120421 aacgtctact tcaacttcat cagatgatcc atctggatat gtcactacaa tagtaccttt
   120481 tttatttcca gctgtgctaa catctacttt ctctttccaa gcataagtag ttccttttgg
   120541 taaatcttct ttgttcttaa ttccctcaga tgcatctggc tctttattta agtctgtagt
   120601 cactttttgt ccttcaggat tgtatttatc tgcattagaa cgattgtctg tcacttctac
   120661 tggaactttc accgtatctt tcgtaccgtc tggatacgtt acttcaatga caccttcata
   120721 attgcctggt gtgttggtat caattgtacc gccgggagta acatctgtta ctgttgttcc
   120781 ttctggtaac gctggcaagt tagtaacatt atctgttaaa tctactgtgc ctccaatttc
   120841 tactttttcg ccttctactg ttggttcata tttatcggcg tcagaacgat tgtctgtcac
   120901 ttctactgga actttcaccg tatctttcgt accgtctgga tacgttactt caatgacacc
   120961 ttcgtaatta cctggtgtgt tggtatcaat tgtaccgccg ggagtaacat ctgttactgt
   121021 tgttccttct ggtaacgctg gcaagttagt aacattatct gttagatcta ctgtgccccc
   121081 aacttctact ttttcgcctt ctactgtt
//
LOCUS       DAYYGY010000002        77334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000002 DAYYGY010000000
VERSION     DAYYGY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.002"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..830)
                     /locus_tag="ACHA52_000113"
     CDS             complement(<1..830)
                     /locus_tag="ACHA52_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8938874.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
     gene            complement(901..1362)
                     /locus_tag="ACHA52_000114"
     CDS             complement(901..1362)
                     /locus_tag="ACHA52_000114"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8938875.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQ
                     LNKYQIYIERNYSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            complement(1326..1451)
                     /locus_tag="ACHA52_000115"
     CDS             complement(1326..1451)
                     /locus_tag="ACHA52_000115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938876.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            complement(1654..2133)
                     /gene="rlmH"
                     /locus_tag="ACHA52_000116"
     CDS             complement(1654..2133)
                     /gene="rlmH"
                     /locus_tag="ACHA52_000116"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8938877.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2561..3850
                     /locus_tag="ACHA52_000117"
     CDS             2561..3850
                     /locus_tag="ACHA52_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8938878.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3889..4494)
                     /gene="ytpR"
                     /locus_tag="ACHA52_000118"
     CDS             complement(3889..4494)
                     /gene="ytpR"
                     /locus_tag="ACHA52_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8938879.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4699..5163)
                     /locus_tag="ACHA52_000119"
     CDS             complement(4699..5163)
                     /locus_tag="ACHA52_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8938880.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5185..5505)
                     /locus_tag="ACHA52_000120"
     CDS             complement(5185..5505)
                     /locus_tag="ACHA52_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8938881.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5649..6728)
                     /gene="pepA"
                     /locus_tag="ACHA52_000121"
     CDS             complement(5649..6728)
                     /gene="pepA"
                     /locus_tag="ACHA52_000121"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8938882.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6928..7278
                     /locus_tag="ACHA52_000122"
     CDS             6928..7278
                     /locus_tag="ACHA52_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8938883.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7501..8448
                     /locus_tag="ACHA52_000123"
     CDS             7501..8448
                     /locus_tag="ACHA52_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8938884.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8505..9923)
                     /gene="pepV"
                     /locus_tag="ACHA52_000124"
     CDS             complement(8505..9923)
                     /gene="pepV"
                     /locus_tag="ACHA52_000124"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8938885.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10072..10917
                     /locus_tag="ACHA52_000125"
     CDS             10072..10917
                     /locus_tag="ACHA52_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8938886.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            10921..11361
                     /locus_tag="ACHA52_000126"
     CDS             10921..11361
                     /locus_tag="ACHA52_000126"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8938887.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11411..11605
                     /locus_tag="ACHA52_000127"
     CDS             11411..11605
                     /locus_tag="ACHA52_000127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938888.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11741..12418
                     /locus_tag="ACHA52_000128"
     CDS             11741..12418
                     /locus_tag="ACHA52_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8938889.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(12826..13515)
                     /locus_tag="ACHA52_000129"
     CDS             complement(12826..13515)
                     /locus_tag="ACHA52_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938890.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13490..15619)
                     /locus_tag="ACHA52_000130"
     CDS             complement(13490..15619)
                     /locus_tag="ACHA52_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8938891.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15667..17004)
                     /locus_tag="ACHA52_000131"
     CDS             complement(15667..17004)
                     /locus_tag="ACHA52_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8938892.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17256..17615)
                     /gene="rplT"
                     /locus_tag="ACHA52_000132"
     CDS             complement(17256..17615)
                     /gene="rplT"
                     /locus_tag="ACHA52_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8938893.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17668..17868)
                     /gene="rpmI"
                     /locus_tag="ACHA52_000133"
     CDS             complement(17668..17868)
                     /gene="rpmI"
                     /locus_tag="ACHA52_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8938894.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(17940..18464)
                     /gene="infC"
                     /locus_tag="ACHA52_000134"
     CDS             complement(17940..18464)
                     /gene="infC"
                     /locus_tag="ACHA52_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8938895.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18508..18633)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            18784..19416
                     /locus_tag="ACHA52_000135"
     CDS             18784..19416
                     /locus_tag="ACHA52_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8938896.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19418..20323)
                     /locus_tag="ACHA52_000136"
     CDS             complement(19418..20323)
                     /locus_tag="ACHA52_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8938897.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20408..21067)
                     /locus_tag="ACHA52_000137"
     CDS             complement(20408..21067)
                     /locus_tag="ACHA52_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8938898.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21383..22327
                     /gene="mvk"
                     /locus_tag="ACHA52_000138"
     CDS             21383..22327
                     /gene="mvk"
                     /locus_tag="ACHA52_000138"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8938899.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22320..23297
                     /gene="mvaD"
                     /locus_tag="ACHA52_000139"
     CDS             22320..23297
                     /gene="mvaD"
                     /locus_tag="ACHA52_000139"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8938900.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23310..24395
                     /locus_tag="ACHA52_000140"
     CDS             23310..24395
                     /locus_tag="ACHA52_000140"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8938901.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24392..25456
                     /gene="fni"
                     /locus_tag="ACHA52_000141"
     CDS             24392..25456
                     /gene="fni"
                     /locus_tag="ACHA52_000141"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8938902.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25705..26508)
                     /locus_tag="ACHA52_000142"
     CDS             complement(25705..26508)
                     /locus_tag="ACHA52_000142"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8938903.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26528..27370)
                     /gene="kduI"
                     /locus_tag="ACHA52_000143"
     CDS             complement(26528..27370)
                     /gene="kduI"
                     /locus_tag="ACHA52_000143"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8938904.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27386..28393)
                     /locus_tag="ACHA52_000144"
     CDS             complement(27386..28393)
                     /locus_tag="ACHA52_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8938905.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28400..29044)
                     /locus_tag="ACHA52_000145"
     CDS             complement(28400..29044)
                     /locus_tag="ACHA52_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8938906.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29144..29902)
                     /locus_tag="ACHA52_000146"
     CDS             complement(29144..29902)
                     /locus_tag="ACHA52_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8938907.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30029..31180)
                     /gene="fucO"
                     /locus_tag="ACHA52_000147"
     CDS             complement(30029..31180)
                     /gene="fucO"
                     /locus_tag="ACHA52_000147"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8938908.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31262..31573)
                     /gene="rhaM"
                     /locus_tag="ACHA52_000148"
     CDS             complement(31262..31573)
                     /gene="rhaM"
                     /locus_tag="ACHA52_000148"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8938909.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31575..32420)
                     /gene="rhaD"
                     /locus_tag="ACHA52_000149"
     CDS             complement(31575..32420)
                     /gene="rhaD"
                     /locus_tag="ACHA52_000149"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8938910.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32434..33690)
                     /gene="rhaA"
                     /locus_tag="ACHA52_000150"
     CDS             complement(32434..33690)
                     /gene="rhaA"
                     /locus_tag="ACHA52_000150"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8938911.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33687..35147)
                     /gene="rhaB"
                     /locus_tag="ACHA52_000151"
     CDS             complement(33687..35147)
                     /gene="rhaB"
                     /locus_tag="ACHA52_000151"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8938912.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35300..36169
                     /locus_tag="ACHA52_000152"
     CDS             35300..36169
                     /locus_tag="ACHA52_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8938913.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36295..37194)
                     /locus_tag="ACHA52_000153"
     CDS             complement(36295..37194)
                     /locus_tag="ACHA52_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8938914.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37359..38183)
                     /locus_tag="ACHA52_000154"
     CDS             complement(37359..38183)
                     /locus_tag="ACHA52_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8938915.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38194..39489)
                     /locus_tag="ACHA52_000155"
     CDS             complement(38194..39489)
                     /locus_tag="ACHA52_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8938916.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39505..39984)
                     /locus_tag="ACHA52_000156"
     CDS             complement(39505..39984)
                     /locus_tag="ACHA52_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8938917.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(39990..41018)
                     /locus_tag="ACHA52_000157"
     CDS             complement(39990..41018)
                     /locus_tag="ACHA52_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8938918.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41035..42141)
                     /locus_tag="ACHA52_000158"
     CDS             complement(41035..42141)
                     /locus_tag="ACHA52_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8938919.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42370..44967)
                     /gene="adhE"
                     /locus_tag="ACHA52_000159"
                     /gene_synonym="adhC"
     CDS             complement(42370..44967)
                     /gene="adhE"
                     /locus_tag="ACHA52_000159"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8938920.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45272..45652)
                     /gene="yajC"
                     /locus_tag="ACHA52_000160"
     CDS             complement(45272..45652)
                     /gene="yajC"
                     /locus_tag="ACHA52_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8938921.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(45749..46894)
                     /gene="tgt"
                     /locus_tag="ACHA52_000161"
     CDS             complement(45749..46894)
                     /gene="tgt"
                     /locus_tag="ACHA52_000161"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8938922.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47112..47810
                     /locus_tag="ACHA52_000162"
     CDS             47112..47810
                     /locus_tag="ACHA52_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8938923.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(47978..50617)
                     /locus_tag="ACHA52_000163"
     CDS             complement(47978..50617)
                     /locus_tag="ACHA52_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8938924.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51236..52267)
                     /gene="queA"
                     /locus_tag="ACHA52_000164"
     CDS             complement(51236..52267)
                     /gene="queA"
                     /locus_tag="ACHA52_000164"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8938925.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52460..53635
                     /locus_tag="ACHA52_000165"
     CDS             52460..53635
                     /locus_tag="ACHA52_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8938926.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            53639..54280
                     /locus_tag="ACHA52_000166"
     CDS             53639..54280
                     /locus_tag="ACHA52_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8938927.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54277..55194
                     /locus_tag="ACHA52_000167"
     CDS             54277..55194
                     /locus_tag="ACHA52_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8938928.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55191..55862
                     /locus_tag="ACHA52_000168"
     CDS             55191..55862
                     /locus_tag="ACHA52_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8938929.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56403..56891)
                     /locus_tag="ACHA52_000169"
     CDS             complement(56403..56891)
                     /locus_tag="ACHA52_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8938930.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57092..57358)
                     /locus_tag="ACHA52_000170"
     CDS             complement(57092..57358)
                     /locus_tag="ACHA52_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8938931.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57358..57621)
                     /locus_tag="ACHA52_000171"
     CDS             complement(57358..57621)
                     /locus_tag="ACHA52_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8938932.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58142..58282
                     /locus_tag="ACHA52_000172"
                     /pseudo
     CDS             58142..58282
                     /locus_tag="ACHA52_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(58424..60247)
                     /locus_tag="ACHA52_000173"
     CDS             complement(58424..60247)
                     /locus_tag="ACHA52_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8938933.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(60845..63550)
                     /locus_tag="ACHA52_000174"
     CDS             complement(60845..63550)
                     /locus_tag="ACHA52_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8938934.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(63856..64803)
                     /locus_tag="ACHA52_000175"
     CDS             complement(63856..64803)
                     /locus_tag="ACHA52_000175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8938935.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            64993..65262
                     /gene="rpsN"
                     /locus_tag="ACHA52_000176"
     CDS             64993..65262
                     /gene="rpsN"
                     /locus_tag="ACHA52_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8938936.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65438..65800)
                     /locus_tag="ACHA52_000177"
     CDS             complement(65438..65800)
                     /locus_tag="ACHA52_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8938937.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(65811..66932)
                     /gene="alr"
                     /locus_tag="ACHA52_000178"
     CDS             complement(65811..66932)
                     /gene="alr"
                     /locus_tag="ACHA52_000178"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8938938.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(66948..67298)
                     /gene="acpS"
                     /locus_tag="ACHA52_000179"
     CDS             complement(66948..67298)
                     /gene="acpS"
                     /locus_tag="ACHA52_000179"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8938939.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67428..68939)
                     /locus_tag="ACHA52_000180"
     CDS             complement(67428..68939)
                     /locus_tag="ACHA52_000180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8938940.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69261..70613)
                     /gene="murF"
                     /locus_tag="ACHA52_000181"
     CDS             complement(69261..70613)
                     /gene="murF"
                     /locus_tag="ACHA52_000181"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8938941.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(70766..71842)
                     /locus_tag="ACHA52_000182"
     CDS             complement(70766..71842)
                     /locus_tag="ACHA52_000182"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8938942.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72038..73783
                     /locus_tag="ACHA52_000183"
     CDS             72038..73783
                     /locus_tag="ACHA52_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8938943.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74063..74500)
                     /locus_tag="ACHA52_000184"
     CDS             complement(74063..74500)
                     /locus_tag="ACHA52_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8938944.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74484..75857)
                     /locus_tag="ACHA52_000185"
     CDS             complement(74484..75857)
                     /locus_tag="ACHA52_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938945.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76179..76667)
                     /locus_tag="ACHA52_000186"
     CDS             complement(76179..76667)
                     /locus_tag="ACHA52_000186"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8938946.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(76689..77291)
                     /locus_tag="ACHA52_000187"
     CDS             complement(76689..77291)
                     /locus_tag="ACHA52_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8938947.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22220 a        16480 c        14093 g        24541 t
ORIGIN      
        1 taaactaatt tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg
       61 atatttctag atatatggac acaacattgt tgaaattgag catttggata aacactatga
      121 atactatcgt caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca
      181 cggtctttta gatcttgaag aatctctttc caaacataag cagattcagt tggagctata
      241 gtaaatccta gaacctcttt agttccatcc aacctgatac caattactat atagacagct
      301 tcttttgata cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag
      361 tatttagctt ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc
      421 atatttgaaa tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag
      481 atttcggaca tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca
      541 ttggttcttt tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt
      601 attactaaat tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg
      661 gaattacctg aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat
      721 tcagcttgta ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa
      781 tcacctttat tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc
      841 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      901 ttacacaaaa tattttacac tctcttcaac gatattcttg taagtaatta tgccccaact
      961 aggatttgtg ggttcatttc catctggagt taaaaaaaca aaaatttttt tatagttaga
     1021 ataattgcgt tctatatata tttggtattt attaagttgc cctccagact ccgaagacag
     1081 aattttattt tcgatgcaca ccactatttt atttttttcc gataccaata aaatatcaat
     1141 attcttattt tctctgtaaa caattaaatc ttcatagcta ttaagtaaag catcaaaata
     1201 attgatagca gctttggtat catcattaga atctatatac cttttcaaaa acattttaaa
     1261 aaactcatca gataaattgt gggtttcatt tggagcaaac aaaaaagcta aaaaattact
     1321 atgtcttatt tcatttttag atagcttaag tatctgaaat atatttgttt tcccagtcca
     1381 tttagatagt tttcttcaat atcatccata tcctctaata gattttttaa acactttttt
     1441 tcatctatca tgttttttct ccttgccatt attctaacta aaattttacg tatttgtttc
     1501 tatgtctaca ttatcttttt aacagtatct atttttcttt tgcaacttac ttggctacca
     1561 aagcaaataa aaaagttatt tataaatttc tccctaccac tacctattgc tattatttaa
     1621 aatagggata attttttctc ttaaaaaata tcattatttg tggtatggag cccccgcatt
     1681 gatccggaag gcacggtaga tttgctcgac aaggatcaga cgcatcaatt gatgtggata
     1741 cgtcattttt ccaaaagaga tctgagcatt gcttcttttc atgacggctt cgctgagtcc
     1801 taaagagccg ccgataacaa aaacaaggtg gctttttcct tggataccta attggtcgat
     1861 ttgtttagca aaagattcac ttgtaggatt tttcccttca attgctaagg catagacgta
     1921 ttcttgttct ttgattttcg ctaagatccg ttccccttct ttttctttta cttgtctcat
     1981 ttgcgcttcg cttaaattct ccggggcttt ttcgtcagga acttcgatca attcgatttt
     2041 tgcataggca ttcagccgtt tgacatactc attgatacct tggatcaggt atttttcttt
     2101 taattttcct actgagataa ttttgatatt catgttttct ctcctttggt cttcatgctc
     2161 actttgttta ttataatcaa ttttttcgtc tatatgttta ttttggctat ttttgttaaa
     2221 aagatttccc caacagttat gcacaacatc cacaggttta tccacagaaa acagtgttat
     2281 tttaccttat ttcatcacat tcattaggca aacatacgtt ttataagaat ttatccactt
     2341 tatacacagg ctgtggatat gttttatccc ttatttttcc aatcctatcc gcgtagtttt
     2401 acacagtatc tgtggaaaaa gaaaatcaaa tattttcatc tgaaaacaat gatttttcct
     2461 aaagtttttc ataaaaagtt cacagtctca aagtagacta aagtcaacaa ataagactat
     2521 actaacagta gataaatgaa tgtaattgga ggaggaacac atggatagaa aaaatgtgac
     2581 accaaagatg aaaaaaagta aaaataatag cctctggcgt aagttaggtc tggggcttgt
     2641 tggagggatc gttggaggac tactgacagc tggcattttt tatgcagcga tgggcactgg
     2701 caacaatagt tctactgcta caagcggcaa tcagaattct gccggtgaaa cagttgtgga
     2761 aaatgtcaaa gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc
     2821 tgtcgtttct gttatcaact tacaaagtca aaaccaatca agcggttttg gcggactatt
     2881 cgggcagcaa gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg
     2941 aagcggcgtg atctacaaaa aagacgggaa tactgcttat gtagtcacga ataatcacgt
     3001 agtagatggc cagcaaggtc ttgaagtctt gatgaaagac ggtacgaagg taaaagctga
     3061 attagttgga acagatgctt attcagattt agcggtgttg aagatcaatt cagacaaagt
     3121 cgaaacagtt gcctcttttg gtgactcaag tgctctgaag gttggtgagc ctgcgattgc
     3181 gattggttcc ccactaggat ctgaatatgc aaattctgtc acttcaggaa tcatttcttc
     3241 cttgaaccga caagtgacaa gtacgaatga atcgaacgaa acagtcaata tcaatgcgat
     3301 ccaaacagat gcagcaatca accctggaaa ttctggtggt cctttagtta atatcgaagg
     3361 acaagttatc ggtatcaact caagtaagat cgcaagtact tctgaatcta cttctaatgt
     3421 aagcgtagaa ggaatgggat ttgctattcc aagtaacgat gtagtgaata tcatcaatca
     3481 actggaaaaa gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc
     3541 tgccgtttct actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg
     3601 tgtcatcgtg acaaatgtcc aaacagctac accggctgaa aaagctggat tgaaacaata
     3661 cgacgtcatt actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc
     3721 tttgtataag aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga
     3781 aaaaacaact actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag
     3841 tgaaaattaa taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa
     3901 tgcttctccg actacggcat cctcaggcaa tactaagatt cctttttttg ctggagcatt
     3961 tggcaaatgc aattcacgag cggaacagat cattccatag ctatctaccc ctcgcaaagt
     4021 tcctggccag atcatcattc catctggcat catagctcct ggttttgcaa cgacaacttt
     4081 ttgtccagct tcgatattgg gtgcaccgca aacgatttgc aatactgtac cattgtctac
     4141 ttctgtctca gtaatcgata aatggtcaga atcaggatgt gggacgcatg tttttacgta
     4201 cccgacaacg atcttcggtt catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt
     4261 gatcaatcga ttcaatttcg ctaattcctc atctgttaag cttacttggc cgttcccttc
     4321 gattgttaga tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc
     4381 tttgatccga gcaacattcc atttgcgttc aacagtgttt tctgttcctt tatcgtcggc
     4441 tataataacc atcaaggtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt
     4501 tcctccatta tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgaaat
     4561 ttttttcatg ctttatttcc gaagaagcct cttctggaac accatttatt cgtaacaagg
     4621 gactgtgaca agacttttgt cacagtccct ttactgtttt agttaattga ttaaaaaggt
     4681 cgtacaagtt ccacttgctt aattacttgg ctgttcttct tcgtcagaaa cgatcaagac
     4741 atcacaaggt gaatgacgta tcacgtagct ggctacactt cctgtcataa agcgttcgac
     4801 tgcatttaga cctgactgcc caaccatgat tagatcgatc tcatattttt ttggcaattt
     4861 gactgccatg gattcacgaa cagaaccata tgcaataatt ccttctatat ttgtgtaatc
     4921 aacagatttc ccatattctt tacattcttc gatcatttcc ttggctgttt cagtttgctg
     4981 atcgactaga ttttgatcaa taggtgcata gcccataaag ttataaaatt gctgatcgat
     5041 tacgtttgct acatatactt tcccattatt acggcgagcc acttctaccg cttttttgaa
     5101 agcagcaagt gcttgttcac taccgtcgat acctactaaa atattcttat attcttgatt
     5161 catcttgacc cctcctaaca gatgtcagcc ttttgttaca ctttctagga acgattcgat
     5221 ttcctgtttt gttttacgat ctttattcac tagacgacct aattcttttc cgtcttggat
     5281 cacgacaaaa cttgggatac caaaaatgtt ccattcagcg gctacatcta tgtacttgtc
     5341 acggtctact tcaataaatt gccaatcaga aaagtcattt tcaatttcag gcatttgcgg
     5401 tttgataaaa cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac tctttccagt
     5461 ttctacgtag cctgctaatt cttctaaact atttgggata atcattcttt gtcctctact
     5521 ttctgtttat gtatttttca attaaattat acacctttct ttcgaactaa caaacattga
     5581 tcctcttttt ataaaaaaaa tctggcaaaa agcaatcttt tgccagattt tatagtttca
     5641 ctatcgcatt attttccgta tacgatcgta tttactgttg atttatccaa acctttcagt
     5701 gtttgaatca acatttctcg tgcagcttca aaatcgcgaa tactgaacat cgtttgatgc
     5761 gtatggatat aacgtcccac tacgccaatc actgtacttg ggattccttc gttttgtgta
     5821 tgagcagcac ctgcatctgt accgcctttt gaaacaaagt attgataagg gatattgttt
     5881 gtttcagcga tatccagcaa gtactcgcgc aaacgaggaa gcatgatcaa acctggatct
     5941 tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt cgtcactgta
     6001 tcatctgctg cagagcagtc tactgcaaag aacagatctg gcttgaattt attgacagaa
     6061 gctttagaac cacgcaagcc tacttcttcc tggacattcg cgcctgcaat caatgtatga
     6121 cctaatgttt cattttgcaa cgcttctaaa gcttctaata ccattgtaca gccatagcgg
     6181 ttgtcccatg atttactgat aatattcttt ccgttggctg ttttgatcgt ttcagcgtaa
     6241 ggaacgattg tgtctcctgg aaggacaccg taactcattg cctcttctct tgattcaaac
     6301 ccagcatcga acaaaatatc ggttacttct actgattttt gtccgcttgt tcctcgcaat
     6361 aaatgtgggg gaacggaaga agaaatacat ggataattct taccagtaga ggttttaagt
     6421 gtaaaacgct gagcagaaac aacgtaagga ttccagccgc ctagaggtac gactttaaaa
     6481 agcccgtttt cttggatttg tgtcagcatg aatccgactt catccatatg tgctgcgacc
     6541 attacacggg gtgcatccgc ttcttcatga tggcgcagac caaaaatgcc gcccaaacca
     6601 tctaactgta actcatctac cagcggttcc atatgacttt ccatataagc gcgaacatct
     6661 tgttcaaaac cgcttgttcc ttgtaattct gttaactctt tgattcgttg aaatgttttt
     6721 tcttccatat gctattcgtc taaatttcag actaaactcc tctcagactt atattgatct
     6781 gcggataaaa tatgattttt tctgcagacc gatcgtattt attatagctt attttttctg
     6841 gtagcggtta tttatttcaa agaaaatatg agagtgtggt aaaataagca caaggagtaa
     6901 caagtgagaa gaaggaggta tgtccacatg tatgaagaaa gcgaactaag ctattttaaa
     6961 ggcgggttag cagttggaat cggtctagga ttattgagcg gggtagcttc aacgatttgg
     7021 tatcatcgca aaaaagtatt gtcagcagac caagtacttg aaaatatcaa gtcatctttt
     7081 ctaaaagaag gtgcaatcga aggttcttgg atctcttttg aaaaggaacc tattaggaaa
     7141 ttcgccgttc ggtcgagtgc ttatcgggga ggtctttctc gaatagaaga tggcgaaact
     7201 gtttattatg aatttttagc agatgctcat acaggaaccg tattagaaat ttcaagaaaa
     7261 aaaggtgcaa cagattaata cgtcaaaaaa gtgagagaac gaatacgcac ccactattca
     7321 aaagagaggt atcatgtcct tcttttgtag tgggtggttt tttagataga gctttccaaa
     7381 catatattcc agtattttct ttttaaataa atgatattta gcgggaaaaa ctagcatttc
     7441 ctttgattat gaggtatttt taatttagct tttgttttct atagttaagg agagtgaaaa
     7501 atggaacaac aagaaaaaat tgctgttctg attccttgtt acaatgagga agcaacaatt
     7561 tcaacagtca ttgcagactt taaacgagaa ttaccagaag cagatattta tgtctatgat
     7621 aataattcga ccgatcgaac atacgaattg gccgttcaag gtggagcaat cgtaaaaaaa
     7681 gaacctcgtc aaggaaaagg aaatgtgatc cgccaaatgt tctttgatat cgatgccgac
     7741 tattatttaa tggtagatgg cgatgatact tatcctgcag aagctgtaca tggtttgctc
     7801 gagaaattac gttctggtga agcagatatg gtcattggtg atcgcctatc aaatggaacc
     7861 tactttgatg agaataagag acctttccac gattttggaa ataaccttgt tagaaataca
     7921 attactagaa tgtataaaac aaaaattctt gatgtcatga ccggctatcg aggattcaat
     7981 cgtatctttg tcaaaagttt tcctattatg agttctggtt ttcaaatcga aacggaattg
     8041 acgatccatg ctttagataa aaaattcaag ttcgttgaaa tcccaatcga ttatcgtgat
     8101 cgtcctgaag gcagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     8161 atgattatta aaatgtttaa agattataag cctttgcttt tctttgggat ttggaccatc
     8221 ttcttctttt tatttggatt ggtcacgggg attcctgtga ttcgcgaata tatgctgaca
     8281 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagcactt
     8341 ctttcattag taacagggtt gattttggat acagtcgtca cgaacgctaa gaaagaatat
     8401 gaattgaatc tatatcatgt ttatcatgaa taccacagta gaaagtaaaa aaaggatcaa
     8461 gactgcaaaa ataatttgca attcttgatc ctctttttaa ttaattattt cgccaaacga
     8521 tagatggcat ctgcatagat tgctgttgct ctgaacaagt catcaagact cataaattcg
     8581 tttgcttgat gcattgtatc agtatatcct gggaacatcg ctccataagc aacgccacgt
     8641 ttcaacaaac gtccgtaagt tccgccaccg atgatttgtt cgtagccttt ttctccagtg
     8701 tggtcttcat agacttgcaa taatgtagcc accaatggat catccattgg tacgtaatgt
     8761 ggttccatta cgcgagcacc tcgagtaact gttgcgcctt cttgccccac tgtttgttcg
     8821 atgtcacttt gcaatccgtc tactgttaca ccttttggaa aacggaaatt catattgatg
     8881 aagttcgctt cctctgtacc attttcttca aaagcaaata gtccagcatt cattgtcaat
     8941 ttgcccattt tttcatcttc ataagcgact cctagttttt ctccataaaa atcttcatgg
     9001 acataagcag ctgctacatg gataaattgt ttagcagatc ctaggaattc atagttatct
     9061 aagaacaatg ccaagaaaga acctgcattg ataccagatt gtgggcttgc accatgtgcg
     9121 cctttaccaa ctaattcgat ttttacatac gtattgtccg cttcaatcgt tccgcttact
     9181 ggatttgctt cgatgaattg gtaaaatgct tcttccatcg cgatagctgc atctgcactt
     9241 ggtacttgaa gtgctgcagt tgctgttcct ggcaccatat tttcgcgaag tccggaaaca
     9301 aaagatttca agacataatc tcctgcattg tcgcctttga agcgtaaagc taaagaaaca
     9361 tttccttttt caccattgat gattgggaat tcagcatctg gtgagaaacc aaaatctggt
     9421 tcttcttcat gttcgaaata ataagccata tcgccccagc cgctttcttc atcgctgccg
     9481 actacgaagc gaactttttt tgatagtttc aagtcaagtt ctttaatgat cttcatcgca
     9541 tagtaagctg ccatacttgg acctttgtca tcgcttgatc cgcgtgcaaa gatctttcca
     9601 tccttgatta ctggttcata aggatctgtg tcccagccat ctcctgcagg aacgacatcc
     9661 atgtgtccaa atatccctag cgtttcgtct ccttcgccaa gatcaatatg ccctgcatag
     9721 ttatcgacat tttttacttt aaatccatca cgttcgccat aagctaacat atgtttcaat
     9781 gcgtcgcgag gtccaggtcc aaatggtgca tctggtgtta cttttgagtc atcgcgctcg
     9841 ctgttgacac gtaaaagatt tttcaagtct tcaagcaagt cttctttacg tgcttctact
     9901 tctttttgcc aatcaattgt cataaagtta atcctccact tatttaagtc ttcgtgttca
     9961 tgataccaca aattacagaa aaaaatagtt caaaaaatct gacaattcct tttcttatct
    10021 gtttttctct aaaaaaaata tacgctataa tgagtaaaag gaggttttta gatgctgata
    10081 aacgaacaac tagtgaaaag agtcatcaca cctcgtccaa aaatttcaca taaaggaaca
    10141 tttggacgta tcgttttgat tggcggcaat gaacaatatg gcggagcaat cattatgagt
    10201 gctttagctg ccgtacacag cggagcgggt ctcacgactg tagtaactga tgaaaaaaat
    10261 cggagtgctc ttcacagtca tctgccagaa gcaatgattg tagtttggac ggatactgac
    10321 cagtttcttt ccgtaataaa gcaagcagat gtactcttga ttggtcccgg attgggtact
    10381 tcttctaaga gtttggaacg tttcaaacaa gtactagaaa accaatcaaa agaacaatgg
    10441 ctggtgattg atggctctgc tttaagttta cttgctgcta atcatctttt gctaccctat
    10501 cccgaacaaa ctgtacttac acctcatcaa atggagtggc aacggttgag ttctctaccg
    10561 atacaagaac aacgggataa agaaaacaaa aagaagcaac aggaaatcgg tgctgtgctc
    10621 gttttgaaaa gtcatgagac aaaaatttat ggtcaccagc acttctacac caatccttta
    10681 ggttctgctg ctatggcaac gggcggcatg ggtgacactt tagctgggat gatcacagga
    10741 tttttagccc agtttcctca aaatgaccaa acgattgcag cagctattta tcttcatagt
    10801 ttcattggag accagctggc aaaagaacaa tatattgttt tgccgacaca aatcagcgaa
    10861 gcacttccga aatggatgaa ttatttttgt aaaaaatcaa tggaaaacga ggcatgagtt
    10921 atggagaaat ttacaggtta tgtgcgagaa cctttttatt atgaaacaga tcaaatgggg
    10981 attattcatc acagcaatta tatccgctgg tttgaagagg ctcgggtagc cttattggta
    11041 catctagggt ttccctataa aaaaatcgaa gaatctggga tcatcatccc cgttctggaa
    11101 gtaaattgca gctataaaga aatgatccat tacggagaaa aagtgagcat ccatccaact
    11161 atccaaaagt atacggggac acggttggat tttagctacg aaattgtggg aactgaagac
    11221 aaaaaattaa aaactactgg cagtagtaaa cactgttttt tactttctga ttcgcaacga
    11281 ttagtgaagc tgagcaaagt caatccggag ttagatcagt tattccaagc gtacgcttct
    11341 ttggatgatg taaattctta ataaagaccg tctaagcatt tttcttgtat tgtgtcgagt
    11401 agttcgtata atgaaaagaa aaaagcaggt gatccccatg tatgtcgtta aagtaatgca
    11461 cggttacatc gataaaactg gctgtcgcac tcgggaaaaa aacttagaca atctgctcat
    11521 tttcaaagac aaaaaagaat ccgaagcttt tgctaaaaga atcggcggtc gtgtcaaacc
    11581 gatccaagag gtacgaccag attgattcaa tatttttctt ttcgaggctc taataggcat
    11641 gcctatcagg gctttttttg cagactgtgt ttttatgccc acacgatttg taagcgattt
    11701 atgttataat agattatatc tgcatagaaa ggaaggttaa atggttatca ataatttgca
    11761 acagcaagct tatgaatcta tacgtaaaca aatcatttat tccgagttag cacctggaag
    11821 taaaatctcc gataaagtgt tagaagaaca actagctatc gggcgtacac cgattcgaga
    11881 agcactgatc caactgcgca atcaagagct tgtaaaaacc attccacagt ccggcactta
    11941 tgtttcaaaa atcgatatcc gatctgcttc attagcccgt tatacgcggg aaaagttgga
    12001 aaaaccgatc ctgcaagaat gcagtgcgaa aatgaccaag caagatcaag ctaaactaga
    12061 aaaaatacta gaacaaaccg atcaggcagt aatcgctcaa aataaaaaag ctttttttga
    12121 tttggatact gattttcacc gaacttgtta tgaaattgct ggaaaacgag aaatttggga
    12181 ttggctagaa agttatagta cgcgtttcag atggttacgt ttgacgattt cagaattaga
    12241 ctggggtcga gtattagatg aacatcagac aatgcttcaa tcaatgattg atcataactt
    12301 tgatgaagtc ggatttcttt gcacgatgca tttacatatg attatcgagg agcaagaata
    12361 cgtgattcat aattaccccg attattttga agatattcag gacagaccca tcaaataaga
    12421 ggttttggaa tcagcgtttt gacctgagta ttagagaaag aaacggcaaa atagttcctc
    12481 atattttgcc gttttttttg agctaatacc gagggtcagc tgattgcaca ccgtttattg
    12541 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaatagctt
    12601 cttatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgtttt
    12661 ccaagaggtc gtgagcctgc cgttcatctc cgagtcacaa ggaacaattt caaaaaacag
    12721 cttctcatgt ttgttgaata ttttgacttg tgaccgagga gttgacagga gaacatcgtt
    12781 tattcggaaa aaaaggactg tgacacgact tttgtcacag tctctttatt tgtaatctaa
    12841 atcatactcc gtacatcttt tttctatttt ttttatttct tctaagatcc cttggctacg
    12901 aatttcatta tatggccaaa gtaagactgc ctctaataca taaggcgaaa aatcgctttg
    12961 attcatctct tttgaaaaac tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg
    13021 atgtgtataa aaaggcaacc gccacagcac aattttttcg ctttgcgtat aaattccctt
    13081 aacagaagct tcgatgtggt gaaatagttc atgtccaata atcaaagctt gcgctgtttc
    13141 tttagagatc gaagctagtc ctgaacattt taatttttct aacggctccg tcatcaatcg
    13201 aatcccttca tcttctgcat aagttgcaaa agtgattcgt tcgtttattt gctctgattc
    13261 acaggaaacg attggaacag ccatttgttc acaaatttct cctacatcgg caaaagggta
    13321 cttttttttg atgctacatg ccgtttcgat accacaatcg attgattgtt gtataagaat
    13381 tttctgcttg gacaaaggaa tctttttcca aaataaatct cgccgaaact ggtagaatcc
    13441 ccaaatttct tcggggatct gttgtaattc gtttagtaac tcttctaact tatgtttgtt
    13501 tgatatttcc ggcaacgacg atcacttctt cctccggttc catagtagat acttccagat
    13561 ccatcaaaag ctgtagttgt tccagatccg tcgctgtaaa atcagagact cttgctccta
    13621 agcagagata aaattctggt cgggcaatca attcagtttg ataaactgcc atatcttccc
    13681 aaagttgttc gacagctgtc atataattgg ctgcagttgt tttcatacat gaagcatgtc
    13741 cacgttggac cttgataaat cctttttgat cgatgatccg aagacttcca gcattttttg
    13801 aacgattctg ttcgccgcat acataaaaga aaggtgtatt ttccaataag caaacttcat
    13861 ttttatttaa tcgcatatcc tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct
    13921 tggttagatc ggttgctttc acttccgtag aaccagtagc gatagccgtt acttttgaag
    13981 tctgtggatc gatttctacg tggacttcga tcgattctgg tgtagcgcct gattcaatcg
    14041 ctttattcat cgcttcattt ttcaacgatc gtatatcttc tttacttggc gaagggatga
    14101 tacgttctac aacatctcgg accattgcaa gtgctacacc aatagaagaa atcacttcgg
    14161 cgttttcagg aatcgaatat ttcacaccca ttttattact aaagtaagta atcaatgagg
    14221 cagcgccgcc ccctacacct acaagagaaa tctgatcttt ctccaagtga tacttgtctg
    14281 ccaattctaa aatcaccggt tcaatcttag cataggattt ttccatgatc tgttctgcga
    14341 tgtcttcaac cgtcgtatga caataatcag ctaatgcttg gatggctttt cgtgcagatg
    14401 ggacatttcc ataagaaaaa tgttcttctt gcactaatcc aaggacattg gctgcacatg
    14461 tatttgtaat agtcacttct tctccgtctt ccatcactac cttgacgtaa tcagcagggt
    14521 ctccttcttt aggagagaaa aattcaacct taggaccttt gattttttct gtctccgtaa
    14581 atactgcata atctagaccg gcaatatgtg cagaacgtgg accgacatcg atgattcctg
    14641 actgattagc ccgtaccatc gaaccgcctg ccacacctag aacccgaaca tctaatgaag
    14701 agatatatgt tgggtggcca cctacaattg aataatcgat tgctggacgt ccatttttaa
    14761 tgaccccgat attggttgtt gttccgccta cttcaaaata aacaccatta gacgctctca
    14821 agtacatcaa agagcccatg actgatgctg ctggaccgga aagcatggtt aaaacaggtc
    14881 gttttttcat ttcattgatt tccattactc cgccgtctcc gcgcataatc atcaaagaaa
    14941 cattgacccc tgcttctcgt acggaatctt ctgtagaggt tgccgtatct agcatttttg
    15001 gtaaaataga tgcattgatt gctgctgtcc gagttcttct tgtcaatcca tataattttg
    15061 tgatgtcaga agccatcgtt gtagggatcg attttaccga tgctgcttca taaacaactc
    15121 gttctggttc cccattatct actccaaatg ccatagaaga aaccaagacc tccgctcttt
    15181 ctctttctaa agaagaaatc gtttctgaaa cacggtctac atttaagtgt ttgacaggta
    15241 agaatgcatt gacaatctct atttttttct tattccctaa atcgatatcg ttcaatctag
    15301 tttgtcgttt tgctaaaaaa ccttctaatc caccttttgc catcccgatt acgccgactt
    15361 ttgctacgtc tccttcaatc agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca
    15421 caacatcttc tggactgata ttattttcca gcaaacaatt ttgaaaagac tgaacgacac
    15481 ctgcggctac tccacggaca tcatcgtggg ttgttttcac tgaagatttt ccgatgattt
    15541 cgtgcgtcgc attatcgatt gcgactgctt tcgtatgtgt accgccaaca tcaattccca
    15601 ttcgcacaaa acgtttcatt ttcttttcct ccattcatct aatcgttttt atcgacgttt
    15661 tgtttactag gcgttcccgt acaataagaa cgtgatcagg cataagatcg ctccgatgat
    15721 ccaaccagtt gggatggatt tcttcatata ttctcgagaa gttacttttg tataacctaa
    15781 tccccaagca acccaagatt gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc
    15841 aaataacggg aacaaaaagg ctggcgacat cgttggatta acagctaata cgacagctaa
    15901 aatagctgag cctgaaccga ctagattcat tggaccgcgg aaaaatccta gtggtgtcaa
    15961 tatagcaaat acgattgcca agactaatgg tgattgcggt atccaatctc caaaaatagc
    16021 tgaaaaataa ggagaagcat acgcagctga attattgaac atcgctaagg tcatcaagaa
    16081 accaaccatt ggcgcaacat ctactgcgcc atctgtaaat aatttcgcaa actgacgaca
    16141 gatttcttta taacctccgc gcaattttcc agtaaacagc aatgcccaga tacctgctag
    16201 aataaaacct aagatgattg gaagatctaa aagaacgacc cctaaaactg gtaataatac
    16261 ggctaaccat gagatcattg gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc
    16321 atagcgtttc tttttgttca ttgatatatt agcgaccgtt agtaccacaa ctaagcttac
    16381 aatcatccca atcataccaa tttggaaata gtcattataa gtatagcttt cagccgctgg
    16441 attgaaacca gcataaatcg tttgatattg tttgaaattg acgatattag caaagattcc
    16501 agccataatc gagcccataa atgagaacag tgcaaccggt actgaaatac ctaatgacat
    16561 caagattggc aacacaataa ctgcaataga aatcactggt ccgattcctg tcatcgacat
    16621 gaatataaca gcggttacaa tacatagtaa gctcatcgtg attcttggct tgtcccctcc
    16681 aagttcaact acctttcgaa tcagggtagc tgcgattccg ctatctacta gaacacgccc
    16741 gaaaaaggca ccaaagaaga catttaccaa gatcgatttg gcgtactcag ctggccccgt
    16801 cgtatagata tgcgtcaaca catcaattac tccttgccct tccatcgccg ggtttggtga
    16861 aaacgtgttt ccgactagcg ctaatcccat ccatacagta gccatgatgg caaagccgac
    16921 catcaagttg tagcctttta cacagtacca gaccatgaaa aagaatgaga taactaacaa
    16981 aataccaata ataatatcac tcatgttatt cctccgtcca ttttgttttt ctgataagca
    17041 aactaatata tcagtcaaac tcattctaac ttatgtagtt agcgctttca ttaaagggtg
    17101 accataaatg ttcagttttc aattaaaaaa ccttaattat gctattttag acttatacgt
    17161 tattgtcctg tttcatttga tggaggattg taaataaaaa aagaggaaag aacataactt
    17221 ttcgttatct tctttcctct ttataattgt ttagcttatt tagccaaagc ttcttttgct
    17281 tggtcagcca atgcagtaaa tgctgctgca tcgttaacag ccaagtcagc taacatttta
    17341 cggttgatgt cgatttcagc aactttcaaa ccgtgcatta attttgagta gctcaagcca
    17401 ttcatacgag ctgctgcgtt gatacgagca atccacaatt tacggaagtc gcgtttcttt
    17461 tgacgacgat cgcgatatgc gtagttgtat gaattcatta cttgttcttt tgctgtttta
    17521 aataaagtgt gttttgaacc gtagtaacct tttgctaatt ttagcactct tttacgacgt
    17581 ttacgagtta ctgttccacc tttaacacgt gccatgtata attcctccta ataaaaataa
    17641 atcttcagat tctgttattt gtacttctta tttcattttt gctagttgtt ggcgaatacg
    17701 tttgaagtcg ccactagaaa ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt
    17761 tttgccgtgg aaacggtgac ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt
    17821 acgtttgaaa cgttttgctg atccgcggtg tgttttttgt tttggcatga ctatatttcc
    17881 tcctcaaaat ctttcgttat ccgactatcg gctgtccaac agccagttag tcattcaaat
    17941 tacttactgt cgtttttcgg tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct
    18001 ttttgttcca ctgttgcaat atccgcagtt tcttcagcta agcgattcaa gactttttga
    18061 ccaatttctt tatgggtaat ggcacggcct ttgaaacgga tagaagcttt cactttatcg
    18121 cccttttcca agaacttacg tgcattacga agttttgtat tgaagtcatt caagtcgatc
    18181 gtaggactta agcgaacttc ttttacattg atcacttttt gttttttacg cgcttcgcgg
    18241 tctttctttt gttgttcgaa acggtattta ccgtagtcca tgattcgcgc aactggtggt
    18301 ttcgcaccag gggcaactaa cacaagatcc aagtttgctt gttctgcaat attcaatgct
    18361 tctgcttttg tcttaactcc taattgctca ccatctgaac cgatcaatcg taactctcgt
    18421 gcacgaatgc cgtcgttaac catcatatcc tttgctatgg tcattcacct ccaaatatta
    18481 tgagagaaag aaaagcttac cgaataaacg gcaataaaaa aacgagttct cttacagaac
    18541 ccgcgcgtac ttcacctaat agaaccagtg aaaactatct agcccagtga cattatcaac
    18601 taaggcgaga agcgggtgct tctgctttta tttctcaacc atcatagtat agcaaaaact
    18661 aatctttttg tcaatgtttt tcatacagtt tgtcttcctt ttctgaaaat gattagatta
    18721 gcagactttc tagcagtgat tcccgcttct ttgctatact aaagaaaaac gaggtgaacc
    18781 cacatgaaaa aaatcgaacc tattccacct tctcttcctc ttcttacaaa gggagagttt
    18841 tatttagaag atgaggagat cttctctggt cttctgatca acgggggtga ccaaagctat
    18901 ctcgatgcca aaaatatggt attaagagag agtcatatcc aaaaacttac gatgcaaaaa
    18961 acagttttac gtcattttga atgcagtaat gtcatttttg aaaaatgtga tttttccaat
    19021 ctggagtgga ttggtggcag tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt
    19081 acgaattttg ccgaaagtta tcttcgagac tgtactttca ctgattgtat tgcttcgatg
    19141 gcttccttta gtagtacaaa cttgaaagct gtctccttcg atcattgtca attagaagac
    19201 agcgaatttt atgaggtcac ttggaaaaac ctttttttgt ctgacaatca gttgaccggt
    19261 tcgaactggt ttcgaacttc tctgaaggga ttagacttta cgactaattt cttcacagca
    19321 atcgctttat ctcaagattc tttggcggga ctaatcgtca accaagagca agcacttgtg
    19381 atcgcccaag cccttggttt gcagatcaaa gactagatca acgaatccgc cgccacagtg
    19441 caataagcat ccaaatagat aagaccaaac tgatgatcaa ggcgattccc catccgaaaa
    19501 cggaatgggt aaagggtaac tggagattca ttccgaagaa tcctgtcaca atagttggaa
    19561 cggtcaagat cagcgaccag accgtcaaaa atttcatcgt gtcattcaag ttgttattca
    19621 gcaaattatt ataagtgcca gagagctggt ctaaaatctg cgaagctaag ttcgtcattt
    19681 caactgcttg tcgtgcttcg atcagcgcat cgtccaactc ttctttttcc ttttctgaca
    19741 acttgcgata aatagccaat gccttgattt gttctaaaag tactacattt tgttttgttc
    19801 ctgtaactaa gtaaaccaaa ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg
    19861 ttgttttttc tcttaatttc agattaagcc gctgacgttc actattcact tcctcgatta
    19921 aaggaaaaaa ggaatcagaa atcaaaaaca atgtacgaaa caataaactg tatacactca
    19981 tgtctggcat tcgttccaaa agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg
    20041 tggtaaatgt aaacaaaccg tcttctttta aaataaaagc gataggacta gtttcataat
    20101 gattatcgac tttttgctgg tgcggaacat ttgatacaag cagtaacgct tcatcaaaag
    20161 aatcgtactc tacgcgcgcc cgttcatttt tatctagaga ataagtcagc atctcgtcac
    20221 taatgccata ttcttctttt aagtttttaa cagctagcac atcgttttgc tgtacattga
    20281 tccaaaaatt acggtcttga ttaaaatagt gtttttcccg catactctat cctcctgaca
    20341 tttgtttcca gtataaagaa aaaagtcgct caaataaagg tttacccttt atttgagcaa
    20401 ctgtaaatca aatggctact ttggtttggc gaatctctgc taatttttct tcgatcattt
    20461 tcaagacttc ttcttttgct tgaggatctt ccacaaaatt atagcggtct ccatcgattt
    20521 ggatttttgg actttctttg tattcttcat accattgatc gtaacgtctg ttcaattctt
    20581 tgtaataatc gtatagactt ggatcaaagg acaattgttc atacgaacga ccacgttttt
    20641 cgatacgctc caacatcgta tcaaaagata cacgaatatg aactaataaa tccggatgtt
    20701 ttttatgggc agcataggga agttcttcca tcatgttttc cagcagttca tcatacacac
    20761 gaacttctgt ttctgttgct cttcctagat ctgcatttag atggaaaagc agtgaatctt
    20821 cataaatcga acggtccaat acattattgt catctttcat tgcttgtttg atgcttgcaa
    20881 aacgtttatt caaaaaataa atttgcagca aaaaagcata ctgttcaggg tttgcataaa
    20941 ataagggcaa gacttcatta tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt
    21001 cagccatcat ttctgttaaa cttgattttc cggcaccaat tgtacctgct aaaacgatca
    21061 cgctcattta cctccagctc acttcatcac taaatattgt gtttaactcc aaccttttta
    21121 aatatacccc caatatctag tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa
    21181 agtcaaaaaa ttcttaagaa aaaagtgttt tttctttaga aaacgtattc ttagttttac
    21241 ttctgagatg gttttagtaa aataaaggag gaatgttttt tgagaccctt agtggacaaa
    21301 aaaattcgtg aagcagcgca actcgttttg ggcgctttat tttttgctta aaaatcactt
    21361 ttacgtgaga aggagttaga ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat
    21421 catattgatg ggcgagcacg cggttgttta tggagaacca gcgattgcct ttcctttcta
    21481 tgcaacaaaa gtcaccgcat tccttgaaga gctggatgca atggacgatc aactggtttc
    21541 ttcctactat tcaggaaatt tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt
    21601 attcattcac ttaaaaaaac agcatgacat ccaaaaaaac ttgcaactga ccattgaaag
    21661 cacgattcct gctgaacgtg gaatgggatc aagcgctgca gtcgccacag cagtcactcg
    21721 tgctttttat gattacttag catttccttt gtctcgtgaa atactattag aaaatgtcca
    21781 gctttcggaa aaaatcgccc atggtaatcc tagtggaatc gatgcagccg ctactagcag
    21841 cttgcagccg atttatttta caaaagggca tcctttcgac tacttttctt tgaacatcga
    21901 tgcttttttg attgtcgctg atacaggaat caaaggacaa acaagagaag ccgtcaaaga
    21961 tgtcgctcac ctctttgaaa cccagcctca tgaaactgga caaatgatcc aaaaattagg
    22021 atacttgacg aagcaagcaa aacaagcaat cattgaaaat tcaccagaaa cgttggcaca
    22081 gacaatggat gaatcacaat cacttctgga aaagctgaca atcagcaatg attttcttaa
    22141 tctattgatc caaacagcaa aagataccgg ggcattgggt gccaaattaa ctggcggtgg
    22201 acgcggtggc tgtatgattg cattagcaca aacaaaaaca aaagcccaag aaatcagtca
    22261 agcacttgaa gatgcgggtg ctgctgaaac ttggatacaa ggattaggag tacataccta
    22321 tgtttaaagg caaagcacgc gcatatacca acattgctct aatcaaatat tggggtaaga
    22381 aaaatgaaga actcatcctt ccaatgaaca acagcctttc gttaacattg gatgcatttt
    22441 atacagagac agaagtcatc ttttctgata gctatatggt agatgaattt tatctagatg
    22501 gcaccttgca agacgaaaaa gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta
    22561 aagaagccgg cctctcacta aaagcttcag taatcagtca gaatttcgtt cctaccgcag
    22621 ctggattagc ctcctctgcc agcgggctag ctgctttagc aggcgcttgc aatactgctc
    22681 ttaagcttgg attagatgat ctctctcttt caagatttgc tcgacgcggg tctggttcag
    22741 cttgccgaag tattttcggt ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt
    22801 cttacgctaa gccagtccct tccgattctt tcgaagacga tttagcaatg gttttcgttt
    22861 tgatcaacga ccagaaaaaa gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa
    22921 catccagttt ttatcaaggc tggttagatt ccgttgaagg ggatctatat caattgaaac
    22981 aagcaatcaa aacaaaagat ttccaacttc tcggagaaac gatggaaaga aacggactaa
    23041 aaatgcatgg aacgacttta gctgcccagc ctccattcac ttactggtct cctgacagct
    23101 taaaagcgat ggatgcggtg cgacaattga gaaagcaagg cattccatgc tactttacga
    23161 tggatgctgg ccctaatgtc aaagttttag tggaaaacag ccatttatct gaggtacaag
    23221 aaacatttac caaattattt agcaaagaac aagtaattac tgcacatgct ggtccaggaa
    23281 ttgcgattat tgaataaagg gagattttta tgattgaagt atctgcacca ggcaaactct
    23341 atattgccgg agaatatgca gttgttgaaa caggccatcc agcagttatc gctgcagtcg
    23401 atcaattcgt gacagtcact gtagaatctg cacgaaaagt cggaagtatc caatctgcac
    23461 aatatagtgg gatgcctgta cgttggacaa gacgcaacgg agaattagtt ttagatattc
    23521 gggaaaatcc ttttcattat atccttgctg ctattcgctt gactgaaaag tatgcacaag
    23581 aaaaaaacat tcttttatcc ttttatgatc tgaaagtaac aagtgagtta gacagttcaa
    23641 atggccggaa atatggattg ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct
    23701 tgaatgtttt ttacgcgttg aatttatctc agttggagat tttcaagatt gcagcactag
    23761 ccaatttagc agttcaagat aatggttcct gcggcgacat cgctgctagc tgttatggtg
    23821 gctggatcgc tttctcaacc ttcgaccatc cttggctcca agaacaagaa actcagcatt
    23881 ctatcagtga gttacttgcc ctggattggc caggtctatc cattgagcca ttgattgctc
    23941 ctgaagattt acgtttattg attggttgga cgggtagccc tgcctctact tctgatttgg
    24001 tcgatcaagt tcaccgttcg agagaagata aaatggtggc ttatcagctt ttcttaaaaa
    24061 atagtacaga atgtgtcaac gaaatgatca aagggtttaa agaaaataat gtaacgttga
    24121 tccaacagat gattcgaaaa aaccgacaat tactgcatga tttatctgca atcactgggg
    24181 tcgtcatcga aacgcctgct ttgaacaaat tgtgtaattt agctgaacag tatgaaggag
    24241 ccgcaaaatc ttctggtgca ggtgggggcg attgcggaat cgtaattgtt gaccagaaat
    24301 ctggcattct tcctttaatg agtgcatggg aaaaagcaga aatcactcca ctgccgttac
    24361 atgtctatag cgatcaaaga aaggaaaacc gatgaatcga aaagacgaac atgtttcact
    24421 agctaaagcc tttcatgata aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa
    24481 ccctttgccg caaatagcag tttctgatgt cgatctaagt acacaggcag ttgggtttac
    24541 actaagcagc ccattttata tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat
    24601 caaccaagat cttgcaatcg tcgcaagaga agcagatttg atgattgcca ctggttcagt
    24661 cagcgctgcc ttgaaagtcc cttctttagc cgatacttat acgattatgc gacaagaata
    24721 tcctcacggg aaaatcattg ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga
    24781 agctatccga ttgtttcacg cagatgcctt acagattcat ttgaatgcac ctcaagagtt
    24841 ggttatgcct gagggagacc gtgattttac caattggaaa gtacttattc aagaaactca
    24901 aacagccatt gacgtccccc tcatcgtaaa agaagtcggt tttggcatga caagagaaac
    24961 gctcaatgat ctagctgctt taggagttca tacagttgat atcagcggtc gaagcggaac
    25021 aagcttcacc cagatcgaaa acgcccgtcg ctccaagcgg gaactgagtt atttagctga
    25081 ctgggggcaa tcgacagtct cttcattgct tgaagcaaat gaagcagaca cttctatgga
    25141 aatcctagcc tccggaggta tccgcaatgc ctatgacata tttaaagccc tttgtctcgg
    25201 cgcaaatgcc gtgggtactt caggaaccgt actgacgcat ctgatgaatc acggcgtaga
    25261 agaaactatt atactgatga agcaatggca agaagagctt cgtctgcttt atactatggt
    25321 cggcgcaacg aataccgcgg ccctacacca acaatctctg atcttttccg gaccagtgaa
    25381 ggattggtgc gaagcaagag ggatcgactt agtgaagtat ggaagacgca cagaaaagag
    25441 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtaca aacggcggaa
    25501 gtaaaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg
    25561 tgaattcctt cttatttctt ggagttaaat gcttctgtcc cagcctcttt tttcgaataa
    25621 atgatgttta atcagctgtc cttcggtatt cgttcaatcc gctgatttca caacctctcc
    25681 actaaacagt gtactaaagc tcttttaagg ttgttttccg atataggcaa gtatgcctcc
    25741 gtccacatac aagacatggc cgttaacgaa atcagatgcg ctagaagcca agaagacaga
    25801 tggtccagcc aaatcgattg gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa
    25861 ttgatcgaat ggatgacgtt caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg
    25921 tgtagcaata taacctggtc caattccatt acattggata ttgtattgac cgtattcaga
    25981 agcgatgttt ttcgtcagca tttttaatcc gccttttgcc gcagcataag cactgaccgt
    26041 ttcgcgtcct aattcactca tcatggagca gatattgatg attttaccgc cgcctttttc
    26101 gatcatatcc ggaataactg cttttgaaac gatgaatggg gcatttaaat ccacgtcgat
    26161 tacttggcgg aagtctgccg catccatttc gatcatcggt gtccgtttga tgatccctgc
    26221 attgttgact agaatatcaa ctgaacctac atcttctttg atctgttgaa ccatcgcttg
    26281 aaccgcttct tcatcggtca catcgcagac ataacctttt gcatcgatgc ctgcttcttt
    26341 ataatgcgct ataccttcat caactgattc ttgattcaag ttattaaata cgatttttgc
    26401 tcctgctgta gctaaagagc gtgcaatctc aaatccaata ccatagacag caccggtgac
    26461 taatgctact tttccatcta aacgaaacat atccatacta aattccatca ttttctctcc
    26521 cttatgctta ttttaattga tccatttgca ccatatccat gtctgtatag gtgatatttt
    26581 ctccgcacat tgcccagatg aatgagtaat tgcttgtacc tacacctgaa tggatcgacc
    26641 agcttggcga aataatggct tgttcattgt ctacaactaa atgttttgtt tcatctggtt
    26701 tgcccatcat gtggaacaca cgtgtatctt cattggcata atcaaagtag acataagctt
    26761 ccatacgacg ttcatgtgta tgacaaggca tcgtattcca agcgcttcct ggttctaaaa
    26821 tcgtataacc catttgtaat tgacagcttt cgcatacatt tggatggatg tattggtaga
    26881 ttttacgttc attcagtgtc aggccttcac cagtctccat tggttttact tggtcgatac
    26941 tgatttttac atttggatat ttatgatgag ctggaacaga actaatatag aatttagctg
    27001 ggttttcagc atcttgagaa gaaaatacaa catgtcgtgt ttcttttccg atatagtatc
    27061 cgtcttgttt tttcatttct tctttttgcc catcgatctc gatgaatcct ggtccaccga
    27121 tattgatcac acctagttca cgacgctcta agaagtaatc aacacctagt tctttgtcta
    27181 atgtgatttc taattctttt tcagtaggtg tcactccacc aaagatcatt cgatcgttgt
    27241 gcgtataagt caaactgatt ttccctggtt caaatacctt ttctactaaa aattcttttc
    27301 tcaattgttc cgttgaataa tgacggatat cttctggact gtgtgtataa cgtgtttcca
    27361 tttcttgcat tttcttctac tccttctatc gaatgatttt tccagagccg catgctaaaa
    27421 attgtgcgac ttcttcttga ctaaattggt tgcagtcccc atggatcgta tgcttcaagg
    27481 cagaagctgc cgtagcaaaa tcaatgattt cttgcggaga catattggat aaaatcccat
    27541 ggagcacgcc acctgcaaaa gcatctccgc cacctactcg atccacgatt ggggtgattc
    27601 tatggacttg ggattccaca tattcatggt tcatccacaa tgtccccttc aattcatttt
    27661 cacttgctga atgaacggta cgcttggtcg aataaaacac ctgaatattc gggaactttt
    27721 cctgcatttt ttgataataa taaattagag gttgttcttc tgcttctggc gctttctcga
    27781 tacctaacaa ataaagagca tccatttttc cagctgagca gatatctact aaaggtaaaa
    27841 gctgatggat tacttcgctg gcttcttgct gtgtccataa ttttccacga tagttgatat
    27901 cgaagctgat caggcagcca gctttttttg cagcttctat cacctgtttc gtcatttttt
    27961 gccatttttt agacaaagct ggggtgattc ctgaaacatg aaatatatct attccttgga
    28021 ataactcctc tagcgaccat tccaaatgat ccatttcagc aaaacttgat ccggcacgat
    28081 catagatcac tgaggcggcg cgttcgccac taccagtttc catataataa gtacctagtc
    28141 ttgatccacc agataatacc tgtttcgtat ctactccata acattgcaag tgctttttga
    28201 ctgcttttcc caatgcgtta tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat
    28261 tagctaaaga aattgctacg tttgcttccc ctccgccata atgggcagcg aatcgttcac
    28321 ttgaatcgat acgggttccc tgctctgtgg acaatcgcaa catgatttct ccgagtgtca
    28381 ctacttttcc cataagcagc tacctcttga tttctttcat ttttgctgca tactgttgcg
    28441 cgacttccgt tactttgtca aaatctcctg tagctgcggg tgctaaaagg ttcccaccta
    28501 ctccgactgt tacgacacca gcatcgaacc actctttcat attgtccaga ctcacaccgc
    28561 cagttggcat gatattgact tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc
    28621 cataagcgct tccaggaaat agtttgacga tatctacccc gcttttcagc gctgttttga
    28681 tctcggtaat agtcatacat cctggtaagt aaggaatttg atacagattg cacatctctg
    28741 ccgtttcttg atcaaaggaa gggctaacaa tatactctgc gccagctaag atagccaagc
    28801 gagctgtgac agcgtctaat actgtgcctg cgccaatcac tatttctggt tggtctttgt
    28861 agaatgacaa aagttctttg attacttgat ctgcttgagg gactgtaaaa gtcaactcaa
    28921 taccagttag accacctttg atgattgcgt ggcaagcatt caaagcttct tctttgcttt
    28981 ttccgcgtac caccgcaatc actccggcgt ttttcaatcg agataaaata tcaatttttt
    29041 tcatcttctc cacctatttc ctatttagaa attttatttt ataacttgga aatctatacc
    29101 catattatac ggcagaaaag aaaaattgca aacgcttttt tcattatttt tgaattcttt
    29161 gctcgatttc gctttttgtt tgcttgaggg cactgattat ttgttctttt gtctcatctg
    29221 ttaaacgata tttcggcata cttacactaa atgcaccaat gatttgttcg tttttcacga
    29281 ttgaggctcc tatacaaaaa atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg
    29341 tttcttttac cgttttgatt tcctttttca accgtaatgc attggtgatc gtatattctg
    29401 tgtaagggat caacggatgc ttttcaagat aatcagtgta ctgctcttca ctgaattctg
    29461 ctaatacggc tttgcccatc gcagaattgt ataatggtcg ggtgattccg atttttgaag
    29521 acatgcggat cgtttgatgt tttggatcta gtttgttcac gtacaagatc tcattgttgt
    29581 ccaatacccc taagtggata gtctcatcga tcttttgctg cagctgttct aaaaacggta
    29641 aagtcaactc taccaaatcg atttgttcaa tgtttttatt cgcgtagcga ataagctttg
    29701 cacctaaacg atagtttttc tctgaatttt tatttacgta gccgatcatc aacaaagtat
    29761 ctagtatttt caagactgta gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag
    29821 agacatcttg gttttccgct aaatagtcta gtattttaga ggctttgatc aatacagttc
    29881 cataaggctt tttattttcc atataatatt ctcctatttc tttataagaa aaattatagc
    29941 ggaaatacca tcaatttaaa agagatatcc agatataaac taccatttct ctttacaggc
    30001 aatttaatgt tgaagatccg cttcgttttt acatcaaact ttgatataaa gcaatgattt
    30061 cttctactgt cgtttctctt ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg
    30121 caattgctgg aatatctgct tcttttacac ctaattcaga aatggtttct gggattccaa
    30181 ccgctttgct caaccgatcg atctcaccac aagccgcttc tcttacatct tctaaagaca
    30241 tttcagctgc tcctttaatc cctagaacaa gagcgatctc acggtacttt tctccagtat
    30301 attctttgtt atatttcatg acagttggca aaagtgctgc acacgctacg ccatgaggaa
    30361 tgttgtacca tgcacttaac gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg
    30421 agaagcccat tcccgcaata tactgtccta atgccatttt ttctcgccca ttttgatcgc
    30481 catctacaga agcttccaaa gaatggccga tgatttcgat tgcttttaaa tgcagcatat
    30541 cagtcatttc ccaagcgcct tttgtgataa acccttcaat agcatgcgtc atagcatcca
    30601 tacctgttga tgcagccaat tttttcggca ttcccatcat catatcgctg tcgatgaacg
    30661 cgacgattgg gatatcatga ggatcaacac aaacaaattt tcggtgattt acttcatctg
    30721 tgatgacgta gttgatcgtt acttctgctg ctgttccaga agtcgttggt atagctaata
    30781 taggtaaaca tggatttttc gtatcagcaa caccttctaa actaatgacg tctgaaaact
    30841 ctggattcgt caaaataatc ccaatggctt ttgccgtatc gattggcgaa cctccaccga
    30901 ttgcgatcaa acagtctgcc tctgcctctt taacaaaagc cactccatct tttacatttt
    30961 ggatcgttgg attaggtacg atcccgtcgt ataaagcata agcgattgct gctttatcta
    31021 gaagttctgt tacttttgtt gctataccgt gctcgatcaa tcctttgtca gtaatgacag
    31081 ctgctttgcc gaatcctcgc tgctgaaatt cagggacgat cgactcaatc gctccttttc
    31141 caaaatacga tgtttcattt aaaatcattc gattactcat tttctattcc tcctaatttg
    31201 tattattttt cacaataaaa acgaaagaac cccaagcaac cggttgccca gttgcttgtt
    31261 gctacagttc aaagactggc atcaattccc aactgactgg agaggcatct ggatttgtct
    31321 ccatgatatc ttccatgtac ttccaccatt tttgattgat agatgtattt gccatttttt
    31381 cccataactt ttcatcttcg atcactaagt aaccaaataa caaacttgta ctcggatcaa
    31441 gatagatcga atagtcgatg accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa
    31501 gttcatcatg tctttttttg tattcttctt gtttttctgg gtaaactttc atgcaaaaag
    31561 cttttttcac catgtcagac acctgctttc gtatgaactt cctccaaata acctgctttt
    31621 ggagtgacac caaacgcatc tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag
    31681 attggtcctt gcgctttgac gtacgtatag acttctgctg ccttttctgc tgtctcgatc
    31741 aaaccaaaga cttcatccat gtcttttcct gacccgtaga ttccatgttg tggccataaa
    31801 accaatcgag tttctttcat tttttcagct gttgcttccc caatctcatt tgttcccgga
    31861 accatccacg gaatgatgga aattccttct ggaaaaacaa ctaagcattc cgtacacatt
    31921 tgccatagcg tacgactaaa ggaacgttcg tctaattcat gcgtaaaact catcgctaat
    31981 agatgactag catggcaatg cataacgatc cgattatctg ggtctacttc taaacgggcg
    32041 atatgactca tgaaatgact tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc
    32101 cacaaaagtt ccagtgtttt gccatcttca gcaactcgaa cgattccaag attctcggca
    32161 ggttcgctcg ctacattttt aaaatatctt cctgaaccag ttacaataaa atagcatcct
    32221 gctaaacggc tggcatcaaa aatcatcgag atgcggcgga tcactttttc aggatccaag
    32281 aacggtgtga tctcttcttc ttttagtaaa taagaaatat tcccgccatt tctttcatcc
    32341 catcccaagc gataaagatt cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt
    32401 aaaatatcta tttttttcaa tatagccaca ctcctatttt cttaattcca acacgtcttt
    32461 ttgatattcc tggacttctt ccagccaatc caaaccaact ggaacttgat ttttgtaaca
    32521 gaaataattc catacatcgg catacgggaa atctttcaat tcttccgtat aagccattct
    32581 tgaagtaaag tcaaaagcca gctccatttc tttcaatcgc tcaactggtt ccagcatagc
    32641 tttcagcagt gctttttgtg tatttctcgt accgatgacc caagcagcga tacggttgat
    32701 cgttgcatca aagaaatcca gaccgatatt tgttttttcc aacaattcgt tacggactaa
    32761 ttctttagcg atttcctgca attcatcatc catcaaaacc acatggtctg aatcccaacg
    32821 cactggacgg gaaacatgca acatgattcc tttgctgaat aaagctaatg aagataattt
    32881 attggaaatc acttctgttg gatgaaaatg tccagcatct agacaaacaa gtttgtcacg
    32941 agtcaacccg taacccatat aaaattcatg ggaaccgact gtataagctt cagcgcctaa
    33001 accaaatagt ttgctttcta ctgcatcttg tacataccgt tcatctaact cttctgtaaa
    33061 aatttcatcg agtgattcca ttaaacgttc ccgtggggta aaacgatcaa tcggattatc
    33121 tttcatccca tcaggtaccc aaaagttatt gatacttgtt tgcccagttt ctttcccaaa
    33181 gtactccgcg attttgcggc ttcttttccc gtgttcgatc cagtattcac gaacttcttt
    33241 atctggatga gccaatgtaa agccgtcttt catcattgga tgcgaaaaga aagtcggatt
    33301 gaagtcaaga ccgatttctt ctttttttgc ccactctacc catttttcaa aatgtttcgg
    33361 ctcaatttca tttagatcga cagattcttc tgtatccaaa tagatagcat gaaggttcaa
    33421 tttgtgtttg cccggtatca acgaataagc cttctccaga tcttgtctaa gctcttcagg
    33481 tgttcgtgcg gctcctggaa aattccctgt cgccatgatt ccaccggtca attcgccatc
    33541 cggtgtcaag aaacctttga catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc
    33601 ctgtatcttt tctagtacag catctgtatc tacaccgata gttgcatatt tttcttttgc
    33661 ttgttcgtaa tttctttcaa tcgtgttcat acgattactc ctttgatttt attcacgtac
    33721 cattccatcg tgttttgctt attttcatct gggtaaaaag tttcacatgg aaacgactgt
    33781 ttgattacat ttcttgcttg ttctatattc gaaaactgac ctgtactgag catttgcatg
    33841 gcaacattcc cgattgctgt ggcttcacta ggtcctgctt ctacaatgat tccggcataa
    33901 tctgctgtca attgattcag tagacgatta ttcgatccgc cccctacgat gtgcagcttt
    33961 tccaatttcg ttccagttaa gtgttctact ttgatcagct cttgtgcgta acaaagagca
    34021 agattatcat acacacaacg agcgatagct cccgatgaga cagggactgg ctgctctgtt
    34081 tcaaaacagt attgctgaat ctcttctatc atatttgggg gattcaaaaa tcgagggtca
    34141 ttgatttcca tcacttgctg cagcggtttt tcttttgcag caagctctgc caattccgca
    34201 taactgtact cgtactcttg cattcgggca acttcctgga tcaaccacat ccccatgatg
    34261 tttttcaaaa aacgcaccgt gttatgggca ccccattcat tcgtataatt ttcagaaaaa
    34321 gcattgcgag aaacattggc gatctttgtt tcaatcccta aaagcgacca tgtcccactg
    34381 ctgatataac cccaacgttc tccttgacct ggcgttccta atacagctga cgccgtatca
    34441 tgacttgcta ctgtgataaa ggtcacatct ggcaaatcat attttgaaaa ttgttctttt
    34501 tgcagatctc ctagtatcgt tcccggctct gtcaatggtg gaaataattc cttcgagaca
    34561 ccgatttttt caagaagttc tatttcccat tcttttgtcc ctgcattcag cagctgcata
    34621 gtagatgcat tagttttctc cgtcaccatg ttaccagtaa agacataccc taaataatca
    34681 ggtatcagta acaatttatg tgcttttgcc aaattttctt tttcttcagc aaacaattgg
    34741 aacaaggtat tgaaaggctg gatctgaatc cctgtctgct catacaactt atctagagaa
    34801 tagatttcag aaaactgttc tactgctcca ttggtacgct tgtcccgata agcaatcgga
    34861 tcatttatcc gatccccttg ttcatcaatc agacagtaat cgactgccca tgtatcgatc
    34921 cccgcaaaaa ggcgatcaat ccctgtctgc tttaattttt caaatccaat caaaatctct
    34981 ttgataagat aatcgatctt ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg
    35041 aaccggtgta cttcttccaa gatcaaagaa ccttttgcat ccctcgtact tttcattagt
    35101 cgtccgcttg atgcaccgat atcgatggca acaaatgttg gattcatttc tccacctctt
    35161 ttactttatt gttgattagc actttattaa taaagcgtta tcatttattg acaatgctat
    35221 cataatacag aatcttatat tttgcttcac actattttgg tatataattg cattatcttg
    35281 acaagttggg agtgtttaca tggattactg ggaaaaagcc aaccactcat ggtcggaaga
    35341 ctcaaaaaga gtgatcttga cgcctacaca aagaagcagg aatttatttt tttatatcca
    35401 agaaatcggt tacttcaaag caccaatccc ttactatact gaacgagcac atttaccttc
    35461 ttacttgata aagtttactt tgtctggcaa aggttcgctt acatataatg ataatcagca
    35521 acagctaaaa gctggagact tattttttat tgactgccaa gaatatcagc gttatcaaac
    35581 gatcagcgaa gaaccatggg aaatggattg gatccatttg aatggtcaga acgtccaatc
    35641 attttatcag gagttcacaa aagacggcca taacaccttc cattcaacag gagatccatc
    35701 agaaaacaag atccaccatt tgatcacaga attgatccac atgcaacaag gcacgaatgc
    35761 ccgtaccgat ttccagtcct ctgtagctat ccacgagttg ctgaatgaac tgattttgca
    35821 aaagtatcag ctagatttcg aagaagagga tattcccgcc tacgtttaca aactgaagca
    35881 atacttagac gaacatttca aagaaacgat tacacttgat caattagaac aacaattctt
    35941 attgaataaa tatcagctga gcaaagattt ttccaaattt atcggttctc caccaatcga
    36001 ttatttgatc agcaaaaaac tttcttacgc taaagacctt cttcggtata cagaatttac
    36061 ggttcgaatg atttccgaag aaatagggat tgagaatttt gcttacttta gccgcctgtt
    36121 caaagcaaaa acaggactat cgccgagaga ctttcgtaaa ttcagctgat tcatataata
    36181 tgtacgtcat tttttcaaaa tattttgaaa tatatagata aaaataaaat aaccgcagtt
    36241 tctgcttcta tgtaaacaac agcagctact gcggttattt tttagtgatc attctcattg
    36301 ttcatcttca aaatgttcat aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc
    36361 tttaggcgtt atgccgaact ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa
    36421 accactttta ctatatacag cactagagct gcggtattct cgcaacaagt gcttggcata
    36481 gatcagcttt ttcttcatta catactgatg aaacgtaaat ccagtgtact ttttaaattc
    36541 tcgtgtgata tggtatttgc tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg
    36601 atgagataaa ttttttgaga tatattgtaa catttgttcc acacgctcat taggaactac
    36661 agcattctgt tcttgcgcac tctcttcttt ttgtacaact tgattgagaa aaatcaagta
    36721 ttctaccaga gcttgttcat attttatgtc ttcaccgtaa atttttttat caggaggatg
    36781 gtcgataaat gataattttt gcttcaatac ttcctgttct atttgcaata cacgactcct
    36841 cttctctccc attggttgaa aacaagccga tagattagac catggcgtgg aacgactttc
    36901 tagaaaagaa ggggtgacaa agatatagac acgctcgaat acatctgaag attggcggac
    36961 gatacgatgc aaatcattgg aatggatgag cagcacactt cctgctttga tcgtaaattg
    37021 tcgtccgttc aaataaaaag tcgcttctcc ctttaatgta gcatggatct catagaaatc
    37081 atgatgatga tagatcgttt tattttggct gacgacatct ttgacatgat agatttcata
    37141 ctgatcagaa accatcgtat tgacttgttc accgctgatt ttcttttgga gcatgtgccg
    37201 cacctccttt tgttcactta tgcacaattt tattatacca cgttttttgt aatcaaaaag
    37261 agctgctttt atatactctt ataaaaaaga agagacgtgg catcttctag ccccgccccc
    37321 tccattctaa atattagtta accaacgtct tctttacatt attcaagaag acttcttcca
    37381 taacgaatcc catcatcttt tgtttcttca agaaccacat acaggtacgg acgattcttc
    37441 atgatgattt ctttgatttt tgtgtactcg atcacaccgt ctcccaaatt taccggcacg
    37501 attttttcct gttcgacacg aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa
    37561 cgttcaaaag cttcttctac caattggact tgttcttgat aatcactact tgtgatcaaa
    37621 ttggtcggat ctaaaatgat tcctagatag tccgaatcta ctgcttggat cagctgctct
    37681 acccttgcta aagaatacac cggatgattc aaacctggtt ctatccccac catcatttgg
    37741 tatttttctc cagcttttac caagcgacgg ataacggaca cagcttcagc aaatgcttca
    37801 tctgtgaaat tttcttccgt atattggatt tccggcaaaa cacatccagt ttccgaagca
    37861 accatcgacg caccaaagga agaagcatag cgaagatact gctcgaactt tgtcagcaac
    37921 tcttgtcgta tcgttaaatc tggatgaatc atattgatat agcaacttaa aattcctacg
    37981 gtcacttctt gcttttctaa aactcgtttg acataatttc ccatcccaga attgatctca
    38041 tttgcgccag aaggtatttc aggaaacgaa agacctaagg cgagctgcag tgtatgaatc
    38101 ccttgttctt ttgttttttc tgcaagatct tctatcgttt ggacttcgga aagatcatgt
    38161 cctctaattg taatgttcaa cataactatt ctcctaactc agcatattgg gtagccacat
    38221 gacgatttgc gggaagtaag taatcaaaaa caagacgact aagataaccc cataaaaagg
    38281 aagaagcggt ttcagcattg cctctgcttt tacgcctcct acacttgcac cgacatatag
    38341 tcctgtacct actggcggcg tgatggagcc tacacacaga ttcatgattg aaaacaaccc
    38401 gtaatgtaca ggatcaaccc ctacacttgt gacgattggt aaaaatattg gtgtaaaaat
    38461 caagatagca ggcgccacat ccataaacat acccaccagt aataacacaa gattgactaa
    38521 taacagaata ataaatttat tatccgttac gcccataatc aacgaactaa ttgcctgagg
    38581 aataccggta aacgccatgg cgaaagacat catggaagaa gtagccaata agaacatgat
    38641 cgtccctgac attgctactg attgaaacaa catattaggt atatctttta gttttaccgt
    38701 tttatagtaa aacatagcaa tcaacaggga gtaaatgaca gctatcgctg aagcttctac
    38761 tgctgtaaat atccccgtca agataccacc gataatgatg acaatcaata gaaggctagg
    38821 aatcgcctct tttattattt ttccagcatg agccatctgt cctttttcga caacagggta
    38881 tttattcttt ttagcaacga tgaatgctac tagcatgatt cctagcgccc acaaagcgcc
    38941 tacgagataa cctcccataa acaaagcaga gatcgaagca ccaccagcaa caagtgaata
    39001 catgataaaa gcagtacttg gaggaatgac catacctgtc ggagctgacg cgatattgac
    39061 cgctgtcgca aacttccggt catatccttc ttccacttgc tgagggatca acactccgcc
    39121 aatcgcggta gaagcggcaa tggccgagct agagatggaa ccaaataaag cattccctaa
    39181 cacattcgta tgagccaaag cccccggcac gcgcccgacg aacaacatcg caaaatccac
    39241 tagtttctta gctatccctc cattattcat gataatgccc gagagaacga aaaacggaat
    39301 agcgatcagc gaaaaactat tgatacttcc caccattttt tgagctgaac taaaagcaga
    39361 aacatcaaac ggtataacta acaataaagt catcatcgat gagaatgcaa tacttatagc
    39421 aatcggcatc cctaatacaa gcaacacaac aaatacaaaa aataacacta atccggcttc
    39481 tattgccatt ttattcctcc atttttatat tttttcttga attgctggct tgttgcttaa
    39541 tgtatcgatt agattgatca ggctatagat aatgatgagt acccccgata caggtactga
    39601 cagataaaca tatcccatag gaatacttaa agaaggcgag atctgcccca ttgtcaatgt
    39661 gacagcattc catccgccat acaccatgat catcgcagca aataccagaa ataaactttg
    39721 aacaacaact tctaaaattt tacttttttt gccagttaat ttttcactta aaaccgttac
    39781 ggctaaatgt gctttttgtc ccacaacata cgcggaagac aacatggcaa accagacaag
    39841 accgaatcga acaagctctt ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa
    39901 tacttgccat aaaacaacag cgaccatccc aacaagcaaa acagctccca ggatctctaa
    39961 taccttgttt aaaatcattt tcatcacagc tacgctcctt gctcttggat cttttgataa
    40021 aggtctttgt aggtaggatc gtttttgaat atatcatgaa gtggggcaac tgcctctcga
    40081 aacggtgtat tatctacatc attgaattgg actccatgtt cttcttgtgc taaagctttt
    40141 tcttcttcaa ttgcttttgc aaaagcttca atttcaaaaa ccgttgattc tttcgctgct
    40201 tcttcaatgg ctttttcctg ctcatcagtt agctgttctt tgatggcatc attcatgatg
    40261 atgatatctg gcacacgtgt atgctcatca taggaatagt atttagccac accaccatgt
    40321 ccagctgtat acaagacaaa ttcattgttt tctgatccgt tgatcaaatt ggattgaaga
    40381 gacgtgtaca cttcatcact tcccataggt acgggcgaac cgcctaaaag ctctaccatt
    40441 ttgatagcgg tttcgctttg catcacacgg atcttctttc ccttcaaatc ttccggcgta
    40501 tggatcggac cgtctaccat ataaaagctg cgctgaccag aatcataata ggtcaatccg
    40561 gtaaatccta aatttttggt cgattggtag attggctcca ttatttcttc attatccatc
    40621 accttaaaga aatgttcttc atcattgaat agataaggaa ctgaaaaaat cgaataatct
    40681 ttagagaaac tttccaacgc tgaggcactt acttttgtga aatctactgc tcctgactgt
    40741 gtcaattcga tcagttcacg ttctccaccc agttgtccat ctgggaaata ctggatctgt
    40801 acggaaccat ctgttttttc ttttactaaa cgcccaaatt cattcattgc atcaattact
    40861 ggctggctgt tgcttgcata agcaaagcgc aaggtaacta tctcatttac atctgcatct
    40921 gcgacagttg ccacactaga attatttccg cagccgctta gacctagcag acctaggcta
    40981 agcgaacttg ctgcaaacag cagtgatgtt tttttcatat gggtttcctt tcttttaaca
    41041 ataagagacg agcaattctg tcaaacaaag caccgttaac gactgtccat aaggcattgc
    41101 tgtgattccg atattccgat aaaaatctaa actgtccccc attcctgtcc cgattgaaac
    41161 gttttgaact tctccttttt catcgatttg ttcaagcaat ccttggatag cacgataagc
    41221 gacttcttta tattcttgtg gtaaataacg tttatggacc gctttcaaaa taccgtaagc
    41281 aaatcctgca gttgcagaag attctacgta agaagaagga tcatccaaca acgtatgcca
    41341 taaaccgctt tcagactgat aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat
    41401 caataatttg cgcaatgcat cttctttcga taattctaag atttcaatga tctctggaat
    41461 agcaatcgtc aaccagcagt ttccacgagc ccaaaatgct tcggcatagt tatgatttcc
    41521 ttcaaacgtc cagccgtgat accacaatcc tgttcgttta tccatcaagt atttgatatg
    41581 gatcataaac tggtaacgag cttcttctaa atattctggg cgattcaaca gtttcccgat
    41641 ttttgccaaa ggaagcactg tcatcatcaa tgtatcgtcc cacaactgat tcttattttc
    41701 cggtccatat gtggcatgct gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac
    41761 ccattctgcc cattgttcta aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa
    41821 gtaagccata gtcaatagcg gtgccattgt attgacgttt ttgggcggag cgccttcttc
    41881 catacgatcc tcaaaccatt cattgaccac ttgataagcg cgaggatttt ttgtcatctg
    41941 gtaattttta taaataccat atagaccgac accttgtggc caattccaca ctgtccagct
    42001 tttgtcatct actttcagtc catcaaagtc caacaaaaat tcaccattat cgtctttgat
    42061 cgttgtcagg ttttcaacga gcaggtcaat tttttctttc actaattctt taggtacaaa
    42121 aatctcttct ttaataatca tctttacgcc acctttttaa acgctttcat tttatatcct
    42181 taatatagca gtaataccgt ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa
    42241 ttgacctaaa tgttaaataa caaaagtaac tttcagttac ttggaaatta aaaaaagaac
    42301 ttggagaaac aacgttttcc aagttctttt tcatctattt tcttatcttt tttctacgct
    42361 ttcttttgct tatccgttgt aagcatcaat gatgatctgt ttcaattcag agatcaatgg
    42421 ttctttcgga ttagctgtcg tacattggtc ttcgtaagct aattcagcca tacggtctac
    42481 ggttgtatct agtgtttctt gtgtgactcc ttgagctttt agactcatgt tgatgccgac
    42541 agattttcct aattcataaa cagccgttgc taaagcttcg actaattcct ctgttgtttc
    42601 gcctttcaga cctaagaatt ttgcgatatc tgcgtaatct gtatctgcac ggaaatagtc
    42661 atatttaggg aacatcgcat gtttttgtgg atcttttgca ttatagcgga taatatgcgg
    42721 taaaagaatc gcatttgtcc gtccatgagg gatcccgtat tccccgccga ttttatgtgc
    42781 gactgagtga caaataccta ggaatgcgtt agcaaacgcc ataccagcca ttgtagaagc
    42841 gttgtgcatt ttttcacgtg attccatatc aggtgttttt actgattttt ctaaatagtc
    42901 aaagactaac ttgattgctt gtaggctcaa tccacgtgta taatctgaag ccatgactga
    42961 aacataggat tcgattgcat gtgttaacac atccatcccg gtatctgctg tgacagatgc
    43021 tggaactgac atgacgaatt gaggatcgac gattgcgaca tctggcgtca atgcataatc
    43081 tgctaacgga tatttcacat gtgtttcact gtcagtgatg accgcaaatg gcgttacttc
    43141 agaaccagtc cctgatgttg tcgggataca tacgaattgt gttttttctg gtttgtcgat
    43201 cttatatgtc cgtttacgga tatccaagaa tttttgtttt gcaccaaaga aagatgtgtc
    43261 tgggtgttca tagaacatcc acattccctt agctgcgtcc attgcagaac cgcctccaag
    43321 agcgattact gtatctggct tgaagtctgc catcagtttc gttcctgcgt atacggtatt
    43381 tgtcgatggg ttcggttcaa catctgagaa gacttcgatc ttcacatcat ttttacgtcg
    43441 tgccaatact tcacgaactg tatcgcagta accgaattgg accataccag ggtcacaaac
    43501 gatcatgaca cgttcaacgt tttccatttt ctctaagtac aatagagagt tcttttcaaa
    43561 gaagattttt ggcggtaatt tgaaccattg catgttattt ctccgtttcg ctacagtttt
    43621 gacattgatt aagtttacag cagagacgtt acgagaaaca gagttcttac cataagaacc
    43681 gcatcccaaa gtcaatgacg gaatcatttc gttatagata tcaccgatgc cgccttctgc
    43741 agatggtgta ttcactaaaa tacgacaagc tttcattcga agaccgaatt tgacatgcaa
    43801 ctcttcgttt tctgaatgga taactgctgt atgtcctaat cctccaagat tcaacatacc
    43861 ttcacacaaa tcaaacgcat ggtccgtgtt gttagctttg atcatggcaa gaactggaga
    43921 aagtttttct cttgatagcg gataatctgg gcctacgcct tccaattcag caatcagcat
    43981 tttcgttcct tttggtacac tgattcctgc caattttgcg atttccatcg cagaatgtcc
    44041 aacgattgat ggattgaccg catattttcc ttcgttcatc actgcatctt ctaatttttg
    44101 caattcatct ggttttacga tataaacttg gtgtgcttgg aattctgctt ttactgctgc
    44161 atagatttct ttatccacga tcacagcttg ttcagaagca caaatcattc cattatcaaa
    44221 tgttttcgaa acgatcaaat cgttcactgc acgtttaatt tttgctgttt tttcaatata
    44281 agctggtacg ttacctggac ctacacctaa agctggtttt cctgtagagt aagcagactt
    44341 gaccattcca gctccacctg tcgctaagac gatcgcgata cctggatggt tcattagcat
    44401 agaagtcgct tcgatcgaag gatgttcgat ccattggata caattttcag gagctccggc
    44461 agcgatagca gcatcacgta ccactcgtgc tgcttctgca gaagattttt gggcacttgg
    44521 atggaaagca aaaacgattg gatttcttgt tttcaacgcg atcattgatt tgaaaatcgt
    44581 tgtggaagtc ggattcgttg ttggagttac tccgcaaacc acaccgactg gttcagcaat
    44641 ttcaatcaac cctttttgga catcttcatt gatcacacca actgttttat catgtttgat
    44701 attatgccaa atatattcgg acgcatacat atttttgatt gctttatctt cgtaaacacc
    44761 gcggcctgtt tcctctacag ccatcttcgc taatggcata tgttgatcca aagctgccat
    44821 tgccatttca tgcacgatat ggtctacttt ttcttgatca aagctttcca tttctttcaa
    44881 tgctgcattt gctttttctg ccaaatcatc aatcattgct tgtacgtcaa ttgtcttcgt
    44941 tttttctttt tctattactt tagccatcat tatcctccta atgggtcatg gttatttgtt
    45001 aaataattca catgtatatc atacaatatt tatttttcta tgtaaacact tttttgtgat
    45061 ttttttcaca gttattttta aatataaaat tgctttattt acaggtgttt gtatagaaaa
    45121 atatataaag ttatttatta ttatttatat gacacacccg attattcaat attgagcaat
    45181 atatcccatg ctcttatctc tttctttttt gaataaaaaa tgaggtagga catctttctg
    45241 ccctacctcg tctttaaata agctttgata cttatttttc ttccttctct tctgtctctt
    45301 ccagcgtttc tgttgtttca gtttttactt caactgtctc agctggttct tctgttgttt
    45361 caacagtagt cgttgcatca tttgttacga cagtccctgg tttgaccgtt ttgattgctg
    45421 cacgatcgta ctcaaggaag atcccttcgc agtctaaaac aactgtccgt ttgtcattgt
    45481 cgatttctga aacaactcca tgtaatccgc cgatcgttac gactgaatca cctggtttca
    45541 tgctatctaa caagttttga cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa
    45601 accacattgc taatagcgcg ataaacatca caagcattgg taatgttccc atttttttca
    45661 cctcactaat tctgtatcta caaaaaacac tttagcagaa aaatgctgat aacactagtt
    45721 ttttacttaa tttttaaaag attttccttt agaagctttt tgcgttttct ttattgaacc
    45781 cgtattcttc aaaaaaggct tctctaaatt caagcaaatt atcatccatg attgcttgac
    45841 gtacttgttt cattagattc aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc
    45901 caaatgtttc atcacatttg atcaaatggc ggatataggc acgtgtatag tttttacatg
    45961 tataacaatc acatttctca tcaattggac ggaaatcatg ggcatatttc gcatttttca
    46021 ctactagacg gccttgagaa gtcatacatg tgccatttcg agcgattcga gtaggcaaga
    46081 cacagtcaaa catgtctact ccacggataa caccatcgat caatgaatca gcagcaccga
    46141 cacccatcaa gtaacgtggt ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac
    46201 gattcatttc tgatttaggt tccccgacag ataacccgcc gattgagtaa ccagggaaat
    46261 ccatcccgac tagatctttc gcgctttgac gacgaagatc ttcaaaccct gctccttgga
    46321 tgattccaaa taatccttga cggtctggat tagcatgagc tttcaatcct cgttctgccc
    46381 atcgagacgt acgttctact gattttttca cgtagtcgta actttcatcg aatggtggac
    46441 attcgtcaaa actcatcatg atatcagagc ccaatttatt ttggatgttg attgcttttt
    46501 ctggtgataa aaacatacgc gaaccgttca agtgattttt gaaatgcacg ccttcttctt
    46561 cgatattccg catatcactt aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg
    46621 gttgatccca gttcataaat ttatgcagtc cgccagcttc ttctactaag tcttcaccag
    46681 ggcgtagcca taaatgatat gtattactta aaataacgcc agcacccatt tctttcaact
    46741 cttctgggga cattgttttt actgtagcta atgttcctac tggcatgaac attggtgtgg
    46801 ggaaagttcc gtgtggcgtg atcaattccc ctaaacgcgc gccagtatgt ttttctttct
    46861 tgattaaacg atagcgaatg gcaggttgcg tcattcattt ccctactttc tatataaaat
    46921 atcttgacaa ctcccaccat cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt
    46981 ccttctactt tatttaatat tctttttttt atcctttttt tatgaataac aaaaaaaaga
    47041 aaaacattat gacagaaata ttttttgata catggtatat tggtgttatt aaagaaaagg
    47101 agggaaaaat catgcaatct tctgcagaat taaaacgcca agcaaaagag agtctaaaag
    47161 gacgttgggg acaagctgtt ttactaaatc taatccctac attgattggt attgctacta
    47221 ttcttcttat tatgattcct attatcttgt ttgccaccac catatatgat ggatcagcga
    47281 gcgtctcaga agcgacaact tcaggaggcg gtagttcagg aggcggtggc ggaattattt
    47341 ccagcatcat tagcgctttg ttcatgagcg gtatttcttg gacttatctt gatattttac
    47401 gtggacaaaa agatcagatc gaaccattca aagatgcatt tcgtggattc aaaggtttgt
    47461 ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat ctttacaaca ttatggggtt
    47521 tgctattagt tattcctgga atcattaaaa gctattctta ttctcaaagc tattttattt
    47581 actacgatat cgtccaagaa acaggacaaa cacctaaagt actggatacg atcactgcaa
    47641 gtcgaaaatt gatggatggt tataaaggac aacttttctg gttagatctt tcatttatcg
    47701 gctggcatat cttagcatta gcaactgctg gtattggtta tctatggctc agcccataca
    47761 tttctgcaac aaaagcagca ttttatgaac aattgccaaa agatatttaa aagagagtgt
    47821 gacttgtgac cgagggggtg gcaggagaac accgtttatc gagaggcagt aaaagaaaca
    47881 ttcagcccat tagcaaaaag gaggtgtgac aacattcatg tcacacctcc tttttgtata
    47941 gttccagtaa aaagaaaatc aacttagaaa cagttaatca ttcctcttct tctgcttccg
    48001 gtaatcgatg gaacttgatc agttcattca ccgcgattgg tacgatagat aaaatcccta
    48061 ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca
    48121 gcaaagcaac agccgttgta atagctaatg ccagtaacgc catttctacc aaagctttat
    48181 tggtcgctaa attgactttg aagatcgatt ggctgctgcg tacattgaac acatgcagaa
    48241 tcgatgaata agctaagatc aagaatgcaa cagtttgacc gacatgctga cttgcgtcta
    48301 cccatgggct gatagtatcg acataactac ctaaataata accaaacaag gttacgatgg
    48361 tgaagacccc tgcatttatg gcaatttttt gcaataaacc tcgagcaaag atcccttcat
    48421 ttttcggaat gggtggctga tccatgatgc cgtgttccgc tggctctctt cctaaagcaa
    48481 atcctggaag tccatcagca actacattga caaacagtag ttgcacagct gtaaatggtg
    48541 ctccccatcc taacagcatc gcaatcaaaa caataaagat ctccgaaata ttacagctta
    48601 gcaaaaagtt gatagcttta cgaatattct gataaaccga acgtccttga ctgacagcgt
    48661 ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc tgcggcactt tgcgccacat
    48721 ctgttcctgt gatccccatg gcacacccaa cgttacttgc tttcaacgct ggtgcatcat
    48781 tcacaccatc cccagtcatc gctacgactg cacccgtacg ttgccaagat ttgacgatcc
    48841 ggatcttgtc ttctggcgtt acccgagcat agacagagag agctttgaca cgcgcatcta
    48901 attcttcgtc tgataattga tgcagttctg atcctgaaag tgcttctgac ggatcattca
    48961 agatacctaa ttctttcgct atggcactgg cagtcaccac atgatctcca gtgatcatga
    49021 ctgttttgat gccagccttt ttcgctcgct tgattgctcc tttactttct ggacgtggtg
    49081 gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa
    49141 tatcttgcgg ttcttcgtca taagtagcat aaccaacagc aatcactctc agtgcccgtt
    49201 taccaaagcg atcattaacg atcgccgctt gttctacatc gcctgaacta aagcgcggag
    49261 caagaacatc gaatgctcct ttggtgattg agatgtattt ttcgcccatt tggtgaactg
    49321 ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga tatttttctt
    49381 ctaattctgc ctttgtatga taattctctt caacagccct tacgatggct acttctgttg
    49441 gattaccttt gataacaagg tcgtcatctt cttgctcgac cgtcacatct gtacatagtg
    49501 cagccatttt caatatttcc atcgcgctgt tcgtcattga atcttcgata ttcgttactt
    49561 catcttcttt gctccatacc cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt
    49621 tgtctgagca gataacatta gcggttccta gcgtttcaac tgctggcaac tgtcggatga
    49681 tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta acaataacag
    49741 tcaaggtttc cggtacagct gcaacagcca aggaaatcga agtcatgaac atctcaagga
    49801 gtggttctcc ttgcagttca ccaatcacaa atactaaagc cgcagccgct aaagcgatca
    49861 tactgattcg tttgcctagc tgattcaatc ggacctgtaa aggtgttttt tgcatagtat
    49921 tttcgttcaa aagatccgcg atcttaccca tttctgtagc catgccaatg gcagtgacga
    49981 tcgctttccc acgacctgca gcaaccgtac atcctttaaa taccatatta agttggtcac
    50041 ctaatgactc atcttcttca gcaataaatg ctgcatcttt ttcaactgct tcactttctc
    50101 cagtcaacgc cgattcttct acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag
    50161 aaatagcatc accggactct aaaacaagaa tatcacccac taccagttgc tctgactcaa
    50221 cagtggtcac tttgccgtct cgtatgacag ttgccgtttg tttattcata tcttgcaagg
    50281 attccagtgc tttttctgcg tttccttctt ggacaatcgc caataccgag ttgatcacaa
    50341 cgatagcgat gatcagcagt ccttcaaata aatccccatg ttctgtagca aatgcggtat
    50401 aaaaggaaat caccgctgct acaagaagga tgatcgttgt aaaatcggac aagcttcgaa
    50461 agaatttttt gatcatcgat tctttcggcg cctcatcgaa tttattcaat ccctgctttt
    50521 ttcgccgttc atccacttct tgtgaagaca acccagtatc tggatctgct tctgttacct
    50581 tctttacttc ttctattgtc tgcttgtatt cttccatcct attcctcctt taaaatcttc
    50641 gtacaaaaaa aggtacttat tttactataa catccttttt ttatttactt caaataaagg
    50701 gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta
    50761 ccaaaggatg ctattatata gatgtaagga aggaggatac catcaaatag acagaagaat
    50821 ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc acccagacat
    50881 gcgtgtagag tacaaaccta taaatagtga atgcgtatgg atgtttggat cacgaagtat
    50941 ccgaaacaag aagtaatgcg atacgtaaca attgaataac gtaaacgtct gaaaattgaa
    51001 taggaacaag agatgtttca aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca
    51061 tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa gttacaatca tttctgttta
    51121 tttgaatcct tccgaggtac aagaaagccg agcttatcga gagctcggct ttttctttgt
    51181 cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca taaccttatt
    51241 tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc gcatgctggt
    51301 aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact aaagtcgatt
    51361 ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag ccaggcgtaa
    51421 tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc gttccaatcg
    51481 tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca tttttacgta
    51541 cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta tgcatatggt
    51601 gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca
    51661 gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc gtaaaatgca
    51721 atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt tgatagcgat
    51781 ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc aatgattcaa
    51841 gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg tgttccaatt
    51901 cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc ccaactttgg
    51961 cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc gttagaagaa
    52021 ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca ggcagaactc
    52081 gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt tcatggaaat
    52141 gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa tgatcgcggt
    52201 cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa tcttctgttg
    52261 ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg tttttcgttc
    52321 tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata aaaaaactac
    52381 cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct tcattgaaaa
    52441 tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg
    52501 aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca tttgttttat
    52561 cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa tgactgatcc
    52621 aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc cagtggaatt
    52681 gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca tgacgatccg
    52741 tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac ggaataaaac
    52801 agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc gctacccaca
    52861 tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca
    52921 agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc gtgattcctt
    52981 gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca
    53041 tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt
    53101 gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga
    53161 gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt
    53221 tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact
    53281 gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg
    53341 atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat
    53401 tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg
    53461 cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg
    53521 tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc
    53581 cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat
    53641 gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta
    53701 tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact
    53761 gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat
    53821 cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc
    53881 gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat
    53941 gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca
    54001 atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt
    54061 agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt
    54121 aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac
    54181 gatccctgta acaattttag cactattggc tgattggctt ctaggtctct tggaacaccg
    54241 gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc
    54301 atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat
    54361 acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca
    54421 agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat catcaacaat
    54481 ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg
    54541 gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg
    54601 aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc
    54661 ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa
    54721 taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta
    54781 gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc
    54841 ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca
    54901 ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag ttatgatcta
    54961 gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac
    55021 aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc
    55081 atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca
    55141 tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat
    55201 tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata
    55261 tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag
    55321 ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga
    55381 ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca
    55441 ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac
    55501 cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg
    55561 gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg
    55621 tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga
    55681 tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa
    55741 cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca
    55801 cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct
    55861 gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta
    55921 gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg ggcaaagagt
    55981 tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc aacgggttca
    56041 tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt
    56101 tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct
    56161 ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc
    56221 agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt
    56281 aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat
    56341 atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat
    56401 tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga
    56461 gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag
    56521 atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg
    56581 actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc
    56641 tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat
    56701 gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc
    56761 aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg
    56821 cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt
    56881 ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa
    56941 ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata
    57001 aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt
    57061 taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa
    57121 tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc
    57181 tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat
    57241 atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct
    57301 tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca
    57361 aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc
    57421 tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca
    57481 gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc
    57541 agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg
    57601 attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt
    57661 gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt
    57721 ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc
    57781 ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa
    57841 aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt
    57901 gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg
    57961 gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt
    58021 tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt
    58081 gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc
    58141 tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga
    58201 aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa
    58261 aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc
    58321 tttaatggct tatagagggc acctttagct gagaataaga caaaacagac cctctagctt
    58381 caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt
    58441 agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac
    58501 ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca
    58561 cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa
    58621 ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat
    58681 gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat
    58741 gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc
    58801 gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat
    58861 cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa
    58921 tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa
    58981 acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc
    59041 ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa
    59101 atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa
    59161 taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc
    59221 gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa
    59281 gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa
    59341 attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa
    59401 cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga
    59461 agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg
    59521 gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac
    59581 cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa
    59641 gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca
    59701 gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc
    59761 tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt
    59821 gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata
    59881 ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct
    59941 ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga
    60001 tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta
    60061 gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac
    60121 catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta
    60181 atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt
    60241 aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt
    60301 atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa
    60361 ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa
    60421 atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac
    60481 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
    60541 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
    60601 aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa aacataagaa
    60661 gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg
    60721 gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag
    60781 gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct
    60841 ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt
    60901 cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga
    60961 tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt
    61021 ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt
    61081 ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc
    61141 ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg
    61201 cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga
    61261 aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca
    61321 tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt
    61381 attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat
    61441 aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct
    61501 caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga
    61561 cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac
    61621 cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt
    61681 cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg
    61741 ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta
    61801 attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc
    61861 cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat
    61921 atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat
    61981 caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga
    62041 cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga
    62101 tgccattgat attattaaca acggcatcaa tttgatctag caattcatca atagccttcg
    62161 tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca
    62221 agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg
    62281 tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc
    62341 cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac
    62401 catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc
    62461 caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct
    62521 gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca
    62581 catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga
    62641 ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct
    62701 taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat
    62761 ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt
    62821 tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt
    62881 gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg
    62941 ttgataaaat catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac
    63001 ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga
    63061 tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt
    63121 tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca
    63181 gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc
    63241 cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa
    63301 gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt
    63361 ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt
    63421 tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg
    63481 caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga
    63541 ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac catgtatttc
    63601 atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt
    63661 gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct ttactttatc
    63721 atgaaataaa agggaaagat aattttatgc caaagttttt atctattata cgtatacgca
    63781 aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt
    63841 ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc
    63901 ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat
    63961 aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc
    64021 tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc
    64081 tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc
    64141 ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa
    64201 ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac
    64261 cttttcctca tttttactgg atgcactggt taagattcgg attccctttt catggagaag
    64321 ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg
    64381 ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc
    64441 ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac
    64501 gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac
    64561 agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata
    64621 agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg
    64681 attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg
    64741 agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg
    64801 catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa
    64861 aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg
    64921 tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg
    64981 aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg
    65041 attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg
    65101 cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga
    65161 cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca
    65221 cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat
    65281 gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct
    65341 tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt
    65401 ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg
    65461 cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca
    65521 aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga
    65581 taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct
    65641 taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac
    65701 ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg
    65761 gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga
    65821 tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat
    65881 tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact
    65941 tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc
    66001 ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc
    66061 cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat
    66121 gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa
    66181 accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta
    66241 agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa
    66301 gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact
    66361 tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa
    66421 aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc
    66481 tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga
    66541 tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac
    66601 cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg
    66661 taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact
    66721 ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct
    66781 gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct
    66841 tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact
    66901 gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa
    66961 ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca
    67021 tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga
    67081 aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa
    67141 cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca
    67201 tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta
    67261 ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa
    67321 tttagaacac tctcattcta ccaaattata accattttgc cacaaataag tccacaaata
    67381 acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt
    67441 gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg
    67501 ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga
    67561 cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt
    67621 ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc
    67681 gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct
    67741 gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca
    67801 gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt
    67861 tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc
    67921 acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct
    67981 ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt
    68041 gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg
    68101 ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct
    68161 tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat
    68221 tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg
    68281 cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga
    68341 tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag
    68401 aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag
    68461 aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt
    68521 ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt
    68581 ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt
    68641 acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat
    68701 tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct
    68761 aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg
    68821 tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa
    68881 ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa
    68941 tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg
    69001 ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta
    69061 gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg
    69121 ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca
    69181 aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt
    69241 ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag
    69301 cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt ttgtttgata
    69361 gcatcaatca atttattctt ttctttttca aaatagaaca caggtagttg ggggtatttt
    69421 tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat
    69481 ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat
    69541 tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta
    69601 tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca
    69661 ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct
    69721 tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca
    69781 ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa
    69841 tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg
    69901 aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg
    69961 ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg
    70021 attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca
    70081 gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt
    70141 tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata
    70201 ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg
    70261 gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct
    70321 actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga
    70381 ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct
    70441 ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg
    70501 aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt
    70561 gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc
    70621 tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc
    70681 acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc
    70741 actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat
    70801 ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca
    70861 tattttccca taagagtggg tacatactga actccgtaaa tcctggcata gagtttaatt
    70921 cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc aatccgcttc
    70981 cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa acttcttctg
    71041 gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct tcataatcat
    71101 agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct tcatttccta
    71161 atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct cgagaatcat
    71221 actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct tttgtaatgc
    71281 cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa gaaccttcac
    71341 attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact ggtacataag
    71401 gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta tccatggcac
    71461 atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct aagaagcctt
    71521 ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg gctccttctt
    71581 ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg
    71641 ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt aatggacctt
    71701 tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa ttataataaa
    71761 tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc
    71821 catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt tcagtgattt
    71881 actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa aaaaacgagc
    71941 cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt gtcccgtttt
    72001 ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag
    72061 ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa actttgtaca
    72121 gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa
    72181 gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca ctcaaacatt
    72241 caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa agacgatcga
    72301 aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat cgccttttgc
    72361 ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt
    72421 taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac tatttgaacg
    72481 agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa aaggggtact
    72541 ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg
    72601 cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca agatttatga
    72661 cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca
    72721 tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa acaagcagct
    72781 gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt ttgatgatgg
    72841 ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa caaaagaaga
    72901 acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg gttcctttgc
    72961 ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca actacatcga
    73021 ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac aagccgtttt
    73081 agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca
    73141 agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc ttgaacagta
    73201 ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg tcgtgacgat
    73261 ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga aagcatttgg
    73321 gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc
    73381 gccttttgct caacattatt ttctcactca tctggatgta gaagatacat tgaaacaaag
    73441 cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac agctccacca
    73501 tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga aaaagcaaga
    73561 caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta tgattttagc
    73621 tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt
    73681 cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta tttttttggc
    73741 aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga
    73801 aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca aatttttgtg
    73861 aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa
    73921 aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa cacatttatc
    73981 ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt cgttacctac
    74041 tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga cgaaaagaca
    74101 tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct tttcttaact
    74161 ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca aattcttttc
    74221 cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa ccataatctt
    74281 gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc gcactatcag
    74341 ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc gtataatggt
    74401 ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt atgacatctt
    74461 cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc cttcttccat
    74521 aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca ttttgccaag
    74581 ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat caataataga
    74641 tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg
    74701 tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg atccattgga
    74761 atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac tacgtgccag
    74821 aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac caataaagaa
    74881 gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt caaaatccat
    74941 ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat aaaaatcgaa
    75001 agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa aaggaaattc
    75061 cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt ccattccttc
    75121 ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc ataaatagca
    75181 agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa caataagcca
    75241 aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt gatgtctttg
    75301 aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac gtaaaaaagt
    75361 gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat cgcttgatac
    75421 aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt gataaaacct
    75481 tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc tgcactcagc
    75541 cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa tattccaagc
    75601 aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact atctagacca
    75661 aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat
    75721 ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag ataaggaata
    75781 aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg cccaaatcgt
    75841 ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat ctgctcttcg
    75901 attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt aaaaaaaaat
    75961 acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact caagcagaca
    76021 agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga taaagataga
    76081 aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga gtccttctcg
    76141 acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc ttttttcatt
    76201 ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc tgtaatctca
    76261 aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac tcgcaaaaag
    76321 atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc
    76381 aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat ttttccattt
    76441 atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa aaagtaggac
    76501 acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt cagcatgtaa
    76561 tccacttgtt tttgaccaat gatcggcaca aaaacttccg gccatacttc ttgtatgact
    76621 gccatcagct catccagttc attttctgtt acttttattt tttccataca cccacgtcct
    76681 tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat catgctatac
    76741 acacccaccg accacggttt caagaaaatc acaatctgca taaattccct aaaagataat
    76801 ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag cagcaaggta
    76861 acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc aaatttctga
    76921 tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg gacaagcaaa
    76981 aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc aaatccattg
    77041 ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg aatgatcact
    77101 tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg ctggataaac
    77161 gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag aatcaacaca
    77221 aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa aactgatcct
    77281 tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc tcct
//
LOCUS       DAYYGY010000003        71211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000003 DAYYGY010000000
VERSION     DAYYGY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.003"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..418
                     /locus_tag="ACHA52_000188"
     CDS             188..418
                     /locus_tag="ACHA52_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8938948.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="ACHA52_000189"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="ACHA52_000189"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8938949.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="ACHA52_000190"
     CDS             1829..2005
                     /locus_tag="ACHA52_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8938950.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="ACHA52_000191"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="ACHA52_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8938951.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="ACHA52_000192"
     CDS             2621..3088
                     /locus_tag="ACHA52_000192"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8938952.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="ACHA52_000193"
     CDS             3173..4057
                     /locus_tag="ACHA52_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8938953.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4171..4407
                     /locus_tag="ACHA52_000194"
     CDS             4171..4407
                     /locus_tag="ACHA52_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8938954.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4495..4575
                     /locus_tag="ACHA52_000195"
     tRNA            4495..4575
                     /locus_tag="ACHA52_000195"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Tyr,seq:gta)
     gene            4582..4653
                     /locus_tag="ACHA52_000196"
     tRNA            4582..4653
                     /locus_tag="ACHA52_000196"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gln,seq:ttg)
     gene            4778..4948
                     /locus_tag="ACHA52_000197"
     CDS             4778..4948
                     /locus_tag="ACHA52_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938955.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5168..5875
                     /locus_tag="ACHA52_000198"
     CDS             5168..5875
                     /locus_tag="ACHA52_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8938956.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5934..6578)
                     /locus_tag="ACHA52_000199"
     CDS             complement(5934..6578)
                     /locus_tag="ACHA52_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8938957.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6704..6991
                     /locus_tag="ACHA52_000200"
     CDS             6704..6991
                     /locus_tag="ACHA52_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8938958.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7099..7419
                     /locus_tag="ACHA52_000201"
     CDS             7099..7419
                     /locus_tag="ACHA52_000201"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8938959.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7500..8813
                     /locus_tag="ACHA52_000202"
     CDS             7500..8813
                     /locus_tag="ACHA52_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8938960.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8810..9529
                     /locus_tag="ACHA52_000203"
     CDS             8810..9529
                     /locus_tag="ACHA52_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8938961.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9657..10208
                     /gene="raiA"
                     /locus_tag="ACHA52_000204"
     CDS             9657..10208
                     /gene="raiA"
                     /locus_tag="ACHA52_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8938962.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10430..12964
                     /gene="secA"
                     /locus_tag="ACHA52_000205"
     CDS             10430..12964
                     /gene="secA"
                     /locus_tag="ACHA52_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8938963.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13090..14191
                     /gene="prfB"
                     /locus_tag="ACHA52_000206"
     CDS             join(13090..13161,13163..14191)
                     /gene="prfB"
                     /locus_tag="ACHA52_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8938964.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14296..14982
                     /gene="ftsE"
                     /locus_tag="ACHA52_000207"
     CDS             14296..14982
                     /gene="ftsE"
                     /locus_tag="ACHA52_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8938965.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14975..15859
                     /gene="ftsX"
                     /locus_tag="ACHA52_000208"
     CDS             14975..15859
                     /gene="ftsX"
                     /locus_tag="ACHA52_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8938966.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16011..17459
                     /gene="cls"
                     /locus_tag="ACHA52_000209"
     CDS             16011..17459
                     /gene="cls"
                     /locus_tag="ACHA52_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8938967.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17796..18653
                     /locus_tag="ACHA52_000210"
     CDS             17796..18653
                     /locus_tag="ACHA52_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8938968.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18768..19688
                     /gene="pstC"
                     /locus_tag="ACHA52_000211"
     CDS             18768..19688
                     /gene="pstC"
                     /locus_tag="ACHA52_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8938969.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19688..20572
                     /gene="pstA"
                     /locus_tag="ACHA52_000212"
     CDS             19688..20572
                     /gene="pstA"
                     /locus_tag="ACHA52_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8938970.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20587..21393
                     /gene="pstB"
                     /locus_tag="ACHA52_000213"
     CDS             20587..21393
                     /gene="pstB"
                     /locus_tag="ACHA52_000213"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8938971.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21409..22167
                     /gene="pstB"
                     /locus_tag="ACHA52_000214"
     CDS             21409..22167
                     /gene="pstB"
                     /locus_tag="ACHA52_000214"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8938972.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22180..22857
                     /gene="phoU"
                     /locus_tag="ACHA52_000215"
     CDS             22180..22857
                     /gene="phoU"
                     /locus_tag="ACHA52_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8938973.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23434..24126
                     /locus_tag="ACHA52_000216"
     CDS             23434..24126
                     /locus_tag="ACHA52_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8938974.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24123..24890
                     /locus_tag="ACHA52_000217"
     CDS             24123..24890
                     /locus_tag="ACHA52_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8938975.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24990..26558
                     /gene="liaX"
                     /locus_tag="ACHA52_000218"
     CDS             24990..26558
                     /gene="liaX"
                     /locus_tag="ACHA52_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8938976.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26855..27175
                     /locus_tag="ACHA52_000219"
     CDS             26855..27175
                     /locus_tag="ACHA52_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8938977.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27175..27525
                     /locus_tag="ACHA52_000220"
     CDS             27175..27525
                     /locus_tag="ACHA52_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8938978.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27692..28630
                     /gene="hprK"
                     /locus_tag="ACHA52_000221"
     CDS             27692..28630
                     /gene="hprK"
                     /locus_tag="ACHA52_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8938979.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28645..29475
                     /gene="lgt"
                     /locus_tag="ACHA52_000222"
     CDS             28645..29475
                     /gene="lgt"
                     /locus_tag="ACHA52_000222"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8938980.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29519..30544
                     /locus_tag="ACHA52_000223"
     CDS             29519..30544
                     /locus_tag="ACHA52_000223"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8938981.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30559..31497
                     /gene="galU"
                     /locus_tag="ACHA52_000224"
     CDS             30559..31497
                     /gene="galU"
                     /locus_tag="ACHA52_000224"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8938982.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31622..32062
                     /locus_tag="ACHA52_000225"
     CDS             31622..32062
                     /locus_tag="ACHA52_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8938983.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32065..32667
                     /locus_tag="ACHA52_000226"
     CDS             32065..32667
                     /locus_tag="ACHA52_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8938984.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32726..33832)
                     /locus_tag="ACHA52_000227"
     CDS             complement(32726..33832)
                     /locus_tag="ACHA52_000227"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8938985.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34042..35043
                     /gene="ccpA"
                     /locus_tag="ACHA52_000228"
     CDS             34042..35043
                     /gene="ccpA"
                     /locus_tag="ACHA52_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8938986.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35380..37725)
                     /locus_tag="ACHA52_000229"
     CDS             complement(35380..37725)
                     /locus_tag="ACHA52_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8938987.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38004..38906
                     /locus_tag="ACHA52_000230"
     CDS             38004..38906
                     /locus_tag="ACHA52_000230"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8938988.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39119..40894
                     /locus_tag="ACHA52_000231"
     CDS             39119..40894
                     /locus_tag="ACHA52_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8938989.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40960..42546)
                     /locus_tag="ACHA52_000232"
     CDS             complement(40960..42546)
                     /locus_tag="ACHA52_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8938990.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42797..43021
                     /locus_tag="ACHA52_000233"
     CDS             42797..43021
                     /locus_tag="ACHA52_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8938991.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43162..44913
                     /locus_tag="ACHA52_000234"
     CDS             43162..44913
                     /locus_tag="ACHA52_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8938992.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44913..46697
                     /locus_tag="ACHA52_000235"
     CDS             44913..46697
                     /locus_tag="ACHA52_000235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8938993.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47023..47874
                     /gene="licT"
                     /locus_tag="ACHA52_000236"
     CDS             47023..47874
                     /gene="licT"
                     /locus_tag="ACHA52_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8938994.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47970..49850
                     /locus_tag="ACHA52_000237"
     CDS             47970..49850
                     /locus_tag="ACHA52_000237"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8938995.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49890..50792)
                     /locus_tag="ACHA52_000238"
     CDS             complement(49890..50792)
                     /locus_tag="ACHA52_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8938996.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51023..52426
                     /locus_tag="ACHA52_000239"
     CDS             51023..52426
                     /locus_tag="ACHA52_000239"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8938997.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52426..53859
                     /locus_tag="ACHA52_000240"
     CDS             52426..53859
                     /locus_tag="ACHA52_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8938998.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            54004..54930
                     /gene="trxB"
                     /locus_tag="ACHA52_000241"
     CDS             54004..54930
                     /gene="trxB"
                     /locus_tag="ACHA52_000241"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8938999.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55144..56871
                     /locus_tag="ACHA52_000242"
     CDS             55144..56871
                     /locus_tag="ACHA52_000242"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8939000.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56886..57236
                     /locus_tag="ACHA52_000243"
     CDS             56886..57236
                     /locus_tag="ACHA52_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8939001.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57328..58188)
                     /locus_tag="ACHA52_000244"
     CDS             complement(57328..58188)
                     /locus_tag="ACHA52_000244"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8939002.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58280..58708
                     /locus_tag="ACHA52_000245"
     CDS             58280..58708
                     /locus_tag="ACHA52_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8939003.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58870..59046
                     /gene="rpsU"
                     /locus_tag="ACHA52_000246"
     CDS             58870..59046
                     /gene="rpsU"
                     /locus_tag="ACHA52_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8939004.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59073..59519
                     /locus_tag="ACHA52_000247"
     CDS             59073..59519
                     /locus_tag="ACHA52_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8939005.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59673..61052)
                     /gene="brnQ"
                     /locus_tag="ACHA52_000248"
     CDS             complement(59673..61052)
                     /gene="brnQ"
                     /locus_tag="ACHA52_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8939006.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            61758..62729
                     /locus_tag="ACHA52_000249"
     CDS             61758..62729
                     /locus_tag="ACHA52_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8939007.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62753..64945
                     /locus_tag="ACHA52_000250"
     CDS             62753..64945
                     /locus_tag="ACHA52_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8939008.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64962..65438
                     /gene="ybeY"
                     /locus_tag="ACHA52_000251"
     CDS             64962..65438
                     /gene="ybeY"
                     /locus_tag="ACHA52_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8939009.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65422..65817
                     /locus_tag="ACHA52_000252"
     CDS             65422..65817
                     /locus_tag="ACHA52_000252"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8939010.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65832..66731
                     /gene="era"
                     /locus_tag="ACHA52_000253"
     CDS             65832..66731
                     /gene="era"
                     /locus_tag="ACHA52_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8939011.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66874..67686
                     /gene="recO"
                     /locus_tag="ACHA52_000254"
     CDS             66874..67686
                     /gene="recO"
                     /locus_tag="ACHA52_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8939012.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68070..68987
                     /gene="glyQ"
                     /locus_tag="ACHA52_000255"
     CDS             68070..68987
                     /gene="glyQ"
                     /locus_tag="ACHA52_000255"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8939013.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            68989..71064
                     /gene="glyS"
                     /locus_tag="ACHA52_000256"
     CDS             68989..71064
                     /gene="glyS"
                     /locus_tag="ACHA52_000256"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8939014.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23285 a        12469 c        15245 g        20212 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga
     4501 gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga
     4561 atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc
     4621 cctcatgcgt tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag
     4681 ttcgagagct cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga
     4741 tagagttaag ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga
     4801 cgaagcagct gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa
     4861 tattccgaaa aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac
     4921 aaaaaaatgg atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc
     4981 atcgatttta gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga
     5041 caataggagt aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta
     5101 tctttttcag ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt
     5161 gtacgttatg tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga
     5221 aatcgaacaa ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt
     5281 gaaattcaat gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg
     5341 aatcttagaa agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa
     5401 aatgtctggt gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag
     5461 tcggacaatc tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt
     5521 gagtaaagat gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat
     5581 ttgttttgaa gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat
     5641 tactgaatcg ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa
     5701 tcaaacgatt actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg
     5761 aggagatgca gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga
     5821 gtatgtgcgt acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac
     5881 aggtgatccc tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata
     5941 tgatcagtta ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg
     6001 ttcagcagct caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag
     6061 atgacgctaa cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga
     6121 taattctgta attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag
     6181 gtaccttcta cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt
     6241 attaatccgc ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca
     6301 ttgatcagtt gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt
     6361 tctccatcat cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag
     6421 ttatggttcg ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc
     6481 tctgattcta ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt
     6541 tgtccatttt cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt
     6601 tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg
     6661 ctgtagtatg cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa
     6721 aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac
     6781 ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga
     6841 aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat
     6901 aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa
     6961 attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa
     7021 tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt
     7081 taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg
     7141 agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct
     7201 gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat
     7261 gtgttgttat taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa
     7321 ccaaaaggca ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa
     7381 aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga
     7441 acaagcgatc caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata
     7501 tgaatcattt ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg
     7561 aggtaaattt accaaattat gagaagatcg cagcagtgga acgtttgagc aactggattc
     7621 tttgtcatcg ctgtggaatg agaacgtccg tcattgatgg acagcttgca gattacggtt
     7681 atttttgtac acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt
     7741 ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt ttggacagga cagcttactg
     7801 aaaaacaaac ggaaatctca aagagaatac tttgccattc agaaaaaaga catcatctta
     7861 tttgggctgt aacaggtgca gggaaaacag agatgcttta tcctatttta gtggaaactt
     7921 taaaagctgg aggaagagta gcgatttgta caccgcgagt cgatgtttgt aatgaattgt
     7981 ttttacgcta tcgttatgtt tttccagaag aaaaaatcac gttgctccat gggaatacag
     8041 caacggctta tacctttact tcttttgtta tctgtacgat tcatcagcta taccgttttt
     8101 atcggtcctt tgatttaata gtgatcgatg agatcgatgc gtttccttat aacggggatg
     8161 caggattagc tcatgctgtg caaacagcga ttaaaccaga cggacgattc atctacttaa
     8221 gtgccacacc tgataaaaag ttactaaagg agatcaagca gacgtttgac atccacaaac
     8281 ttgctttacg ctttcatcgg cgtttgcttc ctgaacccgt cttggttttc atgaataatt
     8341 ggcgacaaaa atgcaagaac aagaagaaga taaaatcttt ggtgcggatc attcatcaat
     8401 taattaaaga aaaccatatc cttttatttt gtccaagtat cacgatgatg catcgtttga
     8461 aagccacact tcaagaggct cttccgagat atgagatcac agatgtttct tcaagagaca
     8521 atcagcgggc ggaaaaagtc cagaaaatgc gagaagcaaa atatgacata ctgttaacca
     8581 cgatgatcct tgaacgtgga gtcacatttg aaagaatatc cgttatcgtt ttaggagcgg
     8641 atcatccggt ttttactaaa tcttctttgg tacaaatcgc aggaagagcc gatcgtaaag
     8701 ggccttatac gaacagtcaa gtatattttt tctatgaaga aaaaactgca gctattcgaa
     8761 aagcttgtca ggaaatcaaa gaaatgaatg aagaagggaa gaagatactg tgaggtgcag
     8821 ttgctgcagt aaagatatcg tccaaaattt aacattcagc gagattattg gtgtttcgaa
     8881 atacagatta cctcagactt gttcagtttg tcgcgcacaa tttcatttat tggaaacaag
     8941 aggctgttct ggctgtcaaa aaccaatcca tttcaatcta gagagtacga aaaagaaagt
     9001 aaattctatc cccgtttact gcagtgactg tcaagaatgg aagaagaaat atccgaccta
     9061 tgattttcag caccgtgcct tttttgtgta cgatgaaaat atgaaggcct ggctcaatca
     9121 atacaagttt atgggagata tccgactagc aggcaccttt gcatataaat ggacgcagat
     9181 aaaaaaagaa tacccgacat acatctattg tccaatacct ttatctgaaa aaaggcgaac
     9241 agaaagagga ttcaatcaag taagcgagat gttgaaagca gcaagaattc cttttcagat
     9301 gctactcaaa agagagaagc atctgctggc tcaagcaaaa aacacgcgaa aggaaagatt
     9361 ggagatgcct caaccttttg aacttgcagt caccttttct aagatacaaa ataaaaaaat
     9421 attgattatt gatgatgtct atacaacagg acagactctt ttccacgcag cagattgttt
     9481 gcagatcgca cagccgaaaa tcatccgaac tatttctttg gcgagatgaa ttagatgtgg
     9541 aaatttgata gcgatttaat ttgcaaataa cctagagttc gatataatag aattaagaag
     9601 agggaaagag tggtccttct ccttcttgat tgtggtagac gaaaggggta atttttatgt
     9661 ttagatataa tgtacgcggc gaaaatatcg aagtaactga agcgattcgc gactacgttg
     9721 agaaaaaagt aggcaaacta gaacgctatt tcagtgacgc acccgatgca acggcgcatg
     9781 tgaacttaaa agtttataca gaaaaaacag caaaagtgga agtaacgatc ccattacctt
     9841 atcttgtatt acgtgccgaa gaaacttctc cagatttata cgcaagtatc gatttggttg
     9901 ttgacaaatt ggaacgccaa atccgcaaat tcaaaacaaa aattaatcgt aaatcccgtg
     9961 aaacgggcat gaatactgca aatgctgctg ctttattcaa tgaagaggac gaaaacgaaa
    10021 atgattctga attagatatc gtacgtacga aacgcctttc attgaaacca atggatagtg
    10081 aagaagcagt cttgcaaatg aacatgctgg gacataactt ctttattttt gaagatgcag
    10141 aaacaaatgg tacaagtatc gtttaccgcc gtaaagatgg aaaatacgga ctgattgaaa
    10201 cagactgatc agccttactt aaataacaac gaaaacttgc agcagctact gacagctgcc
    10261 gcaagttttt ttgagtttcc tcctctttta gatgaaatat gaataaatta tgaagttctt
    10321 actttaatac gcttaagata tttcaattat ctataactaa tgataaaatg aaaagtaaag
    10381 tctacatagt ctggataata cacagaagga aaaggagatt acacaacgca tggccaattt
    10441 gattcgttca cttatcgaaa acgataagaa agaactaaaa cgtttagata gcattgcaaa
    10501 aaaagttgaa gcatacgctg accaaatggc agcattaact gatgaacaat tacaggcaaa
    10561 aactgctgaa ttcaagggca gataccaaaa aggagaaaca ttagatcaat tacttccgga
    10621 agcttttgca gtcgtccgag aagcagcaaa acgtgtatta ggtttatatc cataccacgt
    10681 acaattgatg ggtggaatcg tgcttcacga tggaaatatc cctgaaatgc gtactggtga
    10741 aggtaaaacc ttgaccgcaa cgatgccagt atatctaaat gctctgactg gcgaaggggt
    10801 acacgtagtt accgtcaatg aatatttagc aacccgtgac tcgacggaaa tgggcgaact
    10861 ttataatttc ttaggattaa cagtggggtt gaatatcaac tcaaaatctt cggatgaaaa
    10921 acgcgaggct tataattgtg atatcactta tagtacgaat aatgaattag gatttgacta
    10981 cttgagagac aatatggtcg tttaccgcca tcaaatggta caacgtccgc tgaattatgc
    11041 cattgtcgat gaagttgact caatcttgat tgatgaagcg agaacgccgc tgatcatttc
    11101 agggcaagca gaaaaatcca cagctttata tacaagagca gacaacttcg tgaaacgttt
    11161 gaaagaagaa gaagattata aaatcgatgt acaatcaaaa acaatcagtt taaccgaagc
    11221 agggatcgaa aaagcagaag aaacattcgg tttggataac ttatacgaca ttgaaaatac
    11281 tgcattgacc catcacttgg atcaagcatt gagagccaac tatatcatgc ttttagatat
    11341 cgattatgtt gtccaagacg gaaaagtact gattgttgac cagttcactg gtcggatcat
    11401 ggatggacgc cgttattcag atggacttca ccaagcaatc gaagctaaag aaggcgtaga
    11461 gatcgaagac gaaacgaaaa ccatggcgac gatcactttc cagaactatt tccgtatgta
    11521 caagaaactt gcgggaatga ctggtacagc taaaacagaa gaagaagaat tcagagaaat
    11581 ttataacatc caagtatacc agattccgac aaatcgtccg gttatccgtg atgatcgtcc
    11641 agatttgctt tacccaacat tacaaagcaa gttccacgct gtcgtgcaag atatcaaaga
    11701 tcgctaccgc aaaggtcaac ctgtgttagt cggaactgtt gcggttgaaa catctgagtt
    11761 attatcagat atgctgaatc aagaacgtat tcctcatgaa gtgctgaatg cgaaaaatca
    11821 ctttaaagaa gcagagatca tcatgaatgc tggtcaaaaa ggtgctgtga cgattgctac
    11881 caacatggct ggtcggggaa cagatatcaa gttaggactt ggggtaagag aacttggtgg
    11941 tctagcagtt atcgggacag aacgacatga atctcgtcgt atcgataacc agcttcgagg
    12001 acgtgcagga cgtcaaggag accctggtat gtcacagttc tacttgtcat tggaagatga
    12061 tttgatgaaa cgattcggtt cagaacgaat caaatctttc ttagatcgga tgaagatcgg
    12121 tgaggaagat gctgttatcc aaagcaagat gctgacgaaa caagtagaat ctgcacaaaa
    12181 acgtgtagaa ggaaataact atgacacacg taaaaacgtc ttacaatacg atgatgtgat
    12241 gcgtgaacaa cgtgaagtaa tctataaaca gcgacaagaa gtaatcatgg aagaaaaaag
    12301 cttatccaag ccactaatga acatggtcaa acggacaatc agtcgtgtag tagatgcgca
    12361 tacacaatta gagaaagaaa attggaattt ggaaagtatc atcgattttg ccggcagtac
    12421 tcttgtccat gaagatagta ttactcttgc agatatcgaa ggaaaaacag ctcaggagat
    12481 caaagagtat ctaaacgaac gagcacaaga agtatttaat accaaagcgg ctcaactaaa
    12541 tggtccagag caactgttgg aatttgaaaa agtcgtgatc ttgcgtgttg tggactctaa
    12601 atggacagac catattgatg caatggatca attacgtcag tcaatcggct tacgtgctta
    12661 tggacagaac aatccattag tcgaatatca aacagaaggc tataagatgt ttgaagatat
    12721 ggtaggcgcc atcgagtatg aagtcactcg aatctttatg aaagctgaga tccgtcaaaa
    12781 cgtacagcgt gaacaggttt ctcaaggaac agcttctcac tcagaagacg gagatgccaa
    12841 agaaaatagt aatactagcg cgaaaaaaca gccaatccat gtgaacaagg tcggaagaaa
    12901 tgatccttgt ccttgtggaa gcgggaaaaa atataagaac tgtcatggaa aaaatgcaaa
    12961 ctaatttctg acagcaggat tggtgagaca aattcttttt ttaagaattc gtctcacctt
    13021 ctttctgtta tttgctgatt ctgttagact agatataatg aaaaaagtat agtgaaagaa
    13081 ggaaaaaaca tggaaatatc cgaaattcga aatctcctga cagaaatgca ggaaaaggtc
    13141 acaagcttca ggaggtctct ttgacttaga ccagctggaa gaagacattg cggaagcaga
    13201 agcaaaaatg gctgaacctg gtttttggga tgatagcgaa gctgctcaaa aagtgatcag
    13261 tgaaaacaat gcaaataaag aaacttatga tcaattccat tctcttgctg aagaactgga
    13321 agaaatcgaa ttgatggtgg agatgttgca ggaagaacct gatgcagcca tgcaagaaga
    13381 agcagaaaca cgtatcaatg cgctagatga aaaaatgaag acatatgaat tatctatgtt
    13441 gttagatggt ccttatgatc gaaacaacgc catcgttgaa ttgcatccag gggctggcgg
    13501 aacggagtcc caagattggg gcagtatgct tttacgtatg tatacacgct gggcagagca
    13561 gcatgggtat caagtagaga cactagatta tcaagcaggt gatgaagcag gaatcaagag
    13621 tgtgacccta ctgatcaaag gacacaacgc atttggttat ctgaaatcag aaaaaggtgt
    13681 tcatcgcctt gtacgtatct cgccttttga ctcagcgaaa cgacgtcata cttctttttg
    13741 ttcggtagat gtgatgccag agcttgatga aacgatcgat atagaaatca atccagatga
    13801 tttgaaaatc gatacgtacc gagcaagtgg tgcaggtggt cagcacatca ataaaacaga
    13861 atctgctgta cggatcactc acttgcctac aggaacagta gtagctagtc aggcacaacg
    13921 ttcacaattg aaaaaccgtg agcaagcgat ggggatgtta aaagctaagc tttatcaatt
    13981 agaagtggaa aagaaagaac aagaagcagc tgcacttcga ggagaacaaa aagaaatcgg
    14041 ctggggttca cagatccgtt cttatgtttt ccatccatat tctatggtaa aagaccatcg
    14101 gacaaacgta gagacaggaa atgtccaagc agttatggac ggtgaccttg atttatttat
    14161 tgacggctat ttaaaaatgc atctacaata agaacccatt gttaaattga aataaaattg
    14221 taagattttg caagcatgtt gaaacattgc catgctataa tagctaaaga ctgaaaaaga
    14281 tatggagaga acgccatgat tgaaatgaag gatgtaatga agaagtactc aaatggtacg
    14341 accgctatcc gcaatctctc aattgtcatc gaacaaggag agtttgtcta tgtggttggt
    14401 ccgtctggag ccggtaaatc tacattcatc aaattgatgt atcgagaaga aaaagcttca
    14461 aaaggaactt tgaacgtcgc aggtcatgat ctaatagcaa tcaaaaaccg cgaagttcct
    14521 tatctacgca gagaaatcgg cgtggtgttc caagactata aattattacc aagaaaaaca
    14581 gtatatgaaa atgttgcgta cgctatgcaa gttatcggca gaaaacctcg ggatatcaaa
    14641 aaacgagtaa tggaagtgct ggaccttgtt ggtttgaaac acaaagttcg agtctttcct
    14701 agcgaattat ccggtgggga acagcaacgt gtctctattg ctcgggcaat agtgaatacg
    14761 cctaaagtac ttatcgcgga tgaaccgaca ggaaatctgg atccagaaaa ctcatgggaa
    14821 atcatgaaat tattagatcg gatcaatgcc caaggaacta ctgtcgtgat ggctacacat
    14881 aacagtacga tcgtaaatac gattcggcac cgagtaattg ctatcgaaaa cggccgcatt
    14941 attcgagatc aagcggaagg ggaatatgga tacgatgatt agaacctttt tccgccattt
    15001 attagaaagt atcaagagtt tgaaacgtaa cggctggatg acagttgctt cagtcagtgc
    15061 ggtaacgatc acattgactt tagtcggtgt attcatggca gttatcatga acgcaactaa
    15121 attagctcaa gatatagaag gaaacgttga tgtctctgta ttcgtagata tcggtacaaa
    15181 agacgaagag atgaaaaagc tagaaaaaga attgaatgac ttagatcatg ttaaaaaagt
    15241 tgaattttca agcaaagaac aggaattgaa aaaaatccaa aacgaaatgg gtgattcctg
    15301 gaaattgttt gaaggtgaca ataacccgtt atatgatgtg tatgtagtaa gtgctactga
    15361 tccttcttat acaaaacaag tagcagaaga agcagcagat ttgaagaatg tttctcgttc
    15421 tgattacggt ggagcttcct cagatcggat tttccaaatc gctggtgcag ttcggacatg
    15481 gggattagtt gcagcggcat tgctgctgtt cgtagcgatg tttctcattt cgaacacgat
    15541 tcggatcacg attttgtcta gacagcgtga aattcaaatc atgcgtttag tcggtgcaaa
    15601 aaatggatat attcgctggc cgtttttcct tgaaggtggc tggatcggtc tgatcggtgc
    15661 gattcttccg attttgatca tcacatttgg ttatgcatgg tttttccaat tgatcaatcc
    15721 atcattatta cgttcccatt attcattgat tcatccacaa aatatcgttt ggaaaatcaa
    15781 tcttttaatg gtcggcattg gtgtgatcat tggttcactt ggctcgatca tttctatgag
    15841 acgtttcttg aaattctaat accacaatat aaataaaatg agaaagttgg acagtttcgt
    15901 tccagctttc tttttttatt ggctaggaga atagaaggaa gcggcttttt tccctttact
    15961 ttaaaaaaca acttgggtat tcttaagttg gtagaagaag aggtgaagcc gtgaaaatta
    16021 tcgttgacaa tttttttacg atccttttga ttatgaatat tttgctttcc tttatcatcg
    16081 tttttcgaga aagaaaggaa actgctcaaa catgggcatg ggtgctcgtt ttgatgttca
    16141 ttccagttgt aggatttatt ttatatattt ttttaggaag agggatttcc aaagagaaaa
    16201 tctttgattt gaagatccaa gggaaaatcg gtaagaacct ggaaattgaa gaagacaaac
    16261 aggcattgat gcgagggctt tatcctcatc cgcctactgg acaagtagat gtgaaacaat
    16321 tgatctatat gctcacggta tttgaaagta cgctctatac gactaaaaat gaaatcactt
    16381 tatttacaga tggtcgtgaa aaattcgatg cgctgattga agatatcaag caggcagaag
    16441 accatatcca cttccagtat tatatttatc gcagtgatgc attaggtgaa gaagttcgag
    16501 atgcattgac agaggcggca agacgagggg taaaagtcag agtccttctt gatgcatggg
    16561 gttccacaca agtttcttcc agcttttttc agaatttgaa aaaagcaggc ggagaagtcg
    16621 catttttctt tcctctattt gttccgtata ttaatcctcg gatcaattat cgcaatcatc
    16681 gtaaaatcgt agttattgat gggaaaatcg gatatacagg tgggttcaat gttggaaatg
    16741 aatatctagg attggtaaag aaatttggtt attggcgaga caatcatttg cgtatttacg
    16801 gagaagcggt gtataccttg caaaaccgtt ttttgatgga ctggaattct cagcatacaa
    16861 aagaagtagg ctattctccc aaattttttc cgtccattca ctctactggt gatatagctg
    16921 ttcaaatcgt gacaagcgga ccagataccg aacatgaaca aatcaaaatg acttatttga
    16981 aaatgatttc gttagccaaa cgggagatat tgatccaaac gccttattac ataccggatg
    17041 gatcgattca tgaagcactg aagttggctc ttctttcagg cgtaaaagtt catatccaaa
    17101 tacctaacaa accagatcat cttctcgttt actgggcaac gtattctttc gctgcagagc
    17161 taatcgagta tggagcaagg atcgaaacat atgagaatgg atttattcat gcaaagacga
    17221 tgattatcga tggtggcatt gtttcagttg gctcagcaaa tatcgatgtt cgttcatttc
    17281 gcttggactt tgaggtcaat acactggttt atgatgaacg aatggcttca cgtgtaagaa
    17341 aagctttttt tgaagactcg aaaatttcta cgcatctcac aaaagaaatg tatgaaaacc
    17401 gaggaataat catcaagatg aaagaaggat tagcaagatt gatttcgccg ctcctttaaa
    17461 acataaaaag gacatgagaa ccatcaacag atggagatct cgtgtccttt tctaacgggg
    17521 ccatttctga gagtacgtca gaaatggaga aagcgtctcg catgagcgaa attaactttg
    17581 gggtatctct tgttttttgg ggaaagcaag aaataagcta atatatgcta ttaaaagaga
    17641 cacatttcaa agccagtata gcatagaaac ttttaatgag atagtttttt ttacaattat
    17701 gagaatttat tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata
    17761 aaataaacat actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac
    17821 caataatagg attagctatt cttttatcag gctgttcaaa gtggattgat cgaggagaat
    17881 ccattacggc tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt
    17941 accagacaga aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt
    18001 tgtctcaagt acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa
    18061 aatcggggat cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc
    18121 ctattgtgaa caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat
    18181 ttacaggcga gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat
    18241 tgaaccgcgc ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag
    18301 aaacagctgt tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg
    18361 acacacccgg tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt
    18421 tgaatgttaa cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt
    18481 gggcatatga gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag
    18541 actatatctt atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg
    18601 tttctcatat gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac
    18661 acaaaattta caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat
    18721 ttgtacactg agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga
    18781 aaaaagtatt attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca
    18841 gttttttatg tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta
    18901 gtaaaggatt ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta
    18961 cagattggaa tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta
    19021 tgatcacagg ttcgtttatt gtgacctttt tatctgctat cgttgccacg ccatttgcca
    19081 ttggtgcagc agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc
    19141 cggtcatcga gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag
    19201 ttatcgtgcc ttttgtcaga agtatttttg gcgggactgg gtttggtatc ttagctggta
    19261 catttgtgtt gttcgttatg atcttgccga ctatcacaac gatgactgtt gatgcattaa
    19321 aggcagtgcc acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa
    19381 caatctacaa agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg
    19441 gtatggctcg tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat
    19501 tgatgccaac aggtcttact actcctgctt ctacattgac gtccatctta acaatgggaa
    19561 tcggaaatac aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct
    19621 tgctattgat gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc
    19681 tgaaataatg aacgcaaaac gctcagataa aattgctaca ggtgttttat atatagtttc
    19741 ggggatcatc gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc
    19801 gcacatttca tggtccttct tgaccgaacc ttccaaagcc taccaagaag gcggtggaat
    19861 cgggatccag ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc
    19921 tatttcttta ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctaacaga
    19981 tgtgattcgc acgtcgattg aaatcttgag ctcgttgcct tctgtcgttg tcggtttatt
    20041 cggtttcttg gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt
    20101 ggctttgact gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc
    20161 ggtccattac acacaacgag aagcaggatt ggccttaggt ttgtcacgct gggaaacagt
    20221 gataaaagtg gtggttccag aagcgacacc agggattttg actggtgtga tcctaagttc
    20281 tggaagaatt tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc
    20341 gttagatttt acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca
    20401 agctgaaaca ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg
    20461 tgtttctgtt tcggctggcg cttctgccgt gttgattatt gctgttttac tgttcaactt
    20521 cggcgctcgt gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga
    20581 agcaaaatga aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa
    20641 gaagtagcac tatatacgga tgatctacat gtctggtatg gcgacaatga agcaatcaaa
    20701 ggagtggatc tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc
    20761 ggaaaatcta cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa
    20821 atcactggga aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat
    20881 gagatgcgca aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt
    20941 tatgacaata ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat
    21001 gaaatcgttg aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg
    21061 gacaaaagtg ccttggcttt atctggcggg caacagcaac gtttatgtat tgctcgtgcg
    21121 atcgcgatga aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt
    21181 tcgacaggaa cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc
    21241 gtgacgcata acatgcaaca agcagctcgt atcagcgact atactgcctt tttctattta
    21301 ggaaaagtaa tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca
    21361 accgaagatt atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagag
    21421 attatttcat ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc
    21481 gatctaagct tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcggaaaa
    21541 tccacttatc tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc
    21601 ggaagtgttt tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta
    21661 agaaaagaaa tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa
    21721 aatgtcatct atggattgaa attaaaaggg gaaaaagata aaaaagtcct tgatcaagta
    21781 gttgaagaaa gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa
    21841 agtgctttat ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg tgttttggca
    21901 gttaatccgg aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact
    21961 gggaaaatcg aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc
    22021 cataatatgt ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac
    22081 ttgatcgaat acaatgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa
    22141 gattatattt caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa
    22201 gaagaattgc tgaatctcca taatcagttc tacgaaatgg ggatgatggt gagcagcgcc
    22261 gtgcacaagt cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc
    22321 gaaaacgata taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg
    22381 atcgcattgc aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct
    22441 agctcggatt tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc
    22501 gtaaaagggc agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat
    22561 gtgaaaaaaa tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca
    22621 cgtacgatcg ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca
    22681 acaatcaaaa atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat
    22741 gttgccagtt atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc
    22801 tacttggcta cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact
    22861 gaagctgtga ttatgccgtt tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa
    22921 agctgttctg catcaagtaa taagaacagt caaacaaaaa atagctcctc atatttttcg
    22981 tttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc gggaaagggt
    23041 ttgtgacaaa acatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
    23101 aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag
    23161 catcaagccg ctttatatga agtacctgtc atcgagatag aaaaagtacg aataaaagag
    23221 gatcatcaat tgattacagg aaaactgatg aacacggatc aaaaaggaga atcgatttat
    23281 ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg
    23341 tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg
    23401 cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggtgg aaaacagggt
    23461 ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc
    23521 tttactaaaa cagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg
    23581 atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca
    23641 gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatcattgg
    23701 aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt
    23761 tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc
    23821 attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg
    23881 ctttcaggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc
    23941 gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc
    24001 aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta
    24061 ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa
    24121 cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg
    24181 gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcctgt
    24241 tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat
    24301 tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt
    24361 ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt
    24421 tagcaatcca aggttttaca tcggtagaac tagaagaatt atctggctta gataagactt
    24481 tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg
    24541 ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc
    24601 aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt
    24661 caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt
    24721 ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat
    24781 atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga
    24841 ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac
    24901 ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc
    24961 aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag
    25021 tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg
    25081 aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa
    25141 aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc
    25201 cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg
    25261 aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg
    25321 ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag
    25381 aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa
    25441 tcaaagaatt agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag
    25501 aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt
    25561 ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa
    25621 aaatgtcaga agctggatca cagttaggca aattcttgaa gaaaacattc caaactgttt
    25681 cagaaacagt gaatgacaac atggaatgga aagatgtcag cttacgtgtc cctgggattg
    25741 cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata
    25801 tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg
    25861 aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac
    25921 ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc
    25981 gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc
    26041 taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga
    26101 atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg
    26161 gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta
    26221 cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac
    26281 gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg
    26341 tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa
    26401 gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc
    26461 aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga
    26521 caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc
    26581 agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc
    26641 cttattgccg atagtctatc tcttgaacac cgtttaccaa gagattatga acctgccaat
    26701 tatcgccgag tcacaaggaa taatttcaga aaaagctcgt cacgttttat gaatatggta
    26761 atttgtgacc gagaattttg cagaaagaca ccgattaaga agaaggttgt cacaacgatt
    26821 ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc
    26881 taataaaaat gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga
    26941 tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat
    27001 tattttatac attttattag ctttgattat cccatttgcg cgttctgcac gaacaaacta
    27061 tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac
    27121 aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg
    27181 tactttcaac gtattttagt taatacgtta acattcatat cattggctgt tctttttcct
    27241 gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg
    27301 ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca
    27361 ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg
    27421 ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg
    27481 tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg
    27541 aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat
    27601 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
    27661 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
    27721 agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat
    27781 cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta attattattc
    27841 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
    27901 agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat
    27961 ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaataaaa atgggatagc
    28021 tgtactgcgt tctcccatct caacttctcg tttattagga gaattatcca gttatttaga
    28081 tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg
    28141 tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa
    28201 acgcggccat cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac
    28261 ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat
    28321 cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt
    28381 ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga
    28441 cacgaccgtt gaaatcgcgg aagtgggcat tccacaaata aaaatccctg tcaaaacagg
    28501 acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg
    28561 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
    28621 caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
    28681 gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgctatg
    28741 tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt
    28801 atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat
    28861 cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct
    28921 atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt
    28981 atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc acaggcaatc
    29041 ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc
    29101 ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat
    29161 cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg
    29221 tttttacgaa agaaaccaca ccttttgaaa aaaggcgaag tccttttatg ttatctaatg
    29281 tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta
    29341 ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta
    29401 tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat
    29461 caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
    29521 gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct
    29581 cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
    29641 caacacccaa cacacgaatc aacgttattt accagatcat tatctacccg attccatcca
    29701 agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc
    29761 aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt
    29821 gatcatccat gcaagtaaag gattagaaca gggaacacat aaaaggatct ctgacatctt
    29881 gttagaggaa atcccagcag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca
    29941 tgcagaagaa gtagcaaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa
    30001 agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc
    30061 tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg
    30121 cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt
    30181 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt
    30241 aagcggaata ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc
    30301 aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt
    30361 cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaatga tggaaatcga
    30421 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
    30481 cgcaaaagaa atcatgttac gtgatggaaa aaaagaaaat gaattctcat taacaatttt
    30541 ttgaaggagc gaatgtatat gaaagttaaa aaagcagtta ttccagcagc aggattagga
    30601 acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa
    30661 ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc
    30721 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa
    30781 acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat
    30841 gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa
    30901 gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa
    30961 gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg
    31021 atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga
    31081 gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
    31141 aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat
    31201 gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat
    31261 acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc
    31321 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta
    31381 ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc
    31441 aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc
    31501 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
    31561 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
    31621 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
    31681 ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt
    31741 agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca
    31801 agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc
    31861 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa
    31921 tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc
    31981 gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac
    32041 tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta
    32101 gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa
    32161 cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca
    32221 gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat
    32281 ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca
    32341 gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac
    32401 ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga
    32461 agtattgcta aagatacttc agaagatatc atgattgatg taaaagacgc tgctggtaaa
    32521 gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa
    32581 gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca
    32641 atgacagatt catctacaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc
    32701 ggactagctc tcaccttttt tactcttatt tttctaaaac aagtaattct tttggtgtag
    32761 tcgtgaatgg cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta
    32821 ctttttcagg aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta
    32881 agtcatttcc ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta
    32941 aacggtgatt gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat
    33001 caagttcacc tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa
    33061 gagtgatgtt gtagattttt tcttggaagt cggttggttt ttgataagca attgtacgag
    33121 tagcatcact gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg
    33181 gtgaaatctt cgtatttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga
    33241 ctaatgtgtc gaaagacatt tgtgaaacac cttgtttttt caattggtat tcaatttctg
    33301 caatgatcgc ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa
    33361 catcggccca tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt
    33421 gtagacgttg gatgatggaa gttagatcag tgtaagaatc cgttcccgtc aagatatcag
    33481 agatagcatc gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct
    33541 gaccatagcg agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat
    33601 agccacggac atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag
    33661 tgaaaaggaa aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat
    33721 cactttcgta tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga
    33781 cttggtgttg tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac
    33841 ctactttcta aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac
    33901 gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt
    33961 cttgagatta tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa
    34021 aatatgaata ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga
    34081 gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgttaaa
    34141 ccagcaacaa gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaacgca
    34201 gttgcgcgtg gcttggcaag taagaaaaca acaacagttg gtgtaatcat ccctgatgtc
    34261 agcaatatgt tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa
    34321 tacaatatta tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa tgtattgaat
    34381 aacttactgg ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa
    34441 atccgtggag aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca
    34501 gatgaacaag taggtagcgt aaacattgat tatgtatccg caacaaaaga tgcagtaaac
    34561 aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatccc
    34621 atcaatggac aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca
    34681 tatagcgaag gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagtc
    34741 gatcggatcc ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc
    34801 ggtattttgg atggtttatt agaccgtggc gtgaaagtac cggaagaatt cgaaatcatc
    34861 acaagtaaca attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa
    34921 ccattatacg atttaggggc ggtatctatg cgcctattga cgaaaatgat gaataaagaa
    34981 gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa
    35041 taataaaagg acaaccattc agaggttgtg tgcctgccgt ttaactctgc gttacaagga
    35101 gcaatttcaa aaaacagcat ctcatgtttg atgaatattg tgactcgtga ttcggagttg
    35161 gcaggagaac accgtttatt tgggaaaagg ggttatgaca aaattttgtt ataatccttt
    35221 ttttgtgtct taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat
    35281 ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa aataagggac tgtgacaata
    35341 ctattgtcac aatcccttat tactgtgtat tctattaaat caatcatctt tctcatctga
    35401 ttctttagtt gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata
    35461 gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc
    35521 ccatagcgat tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt
    35581 tccaggtgtt tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc
    35641 gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt
    35701 ggcaagataa ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg
    35761 ccctgcccag ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta
    35821 ttcattggta gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct
    35881 tgagatggca tctttgaatg gtgtagtgat ttttgcattg atcacactac gcatcatgtc
    35941 attcatgatc gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata
    36001 aattacattt cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc
    36061 gccgtttgct aaagcctgaa atccgttggt ctgttgaagc gtcgtgacat ctactgtacc
    36121 taaaggagca gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta
    36181 gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata
    36241 agccgggata ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc
    36301 ttcgttagta gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc
    36361 ataatctgat acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac
    36421 aagaactggt ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg
    36481 attctgactg taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat
    36541 caatacattt ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt
    36601 ttcaacgcca ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata
    36661 tcctttattt tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc
    36721 agacatatca gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt
    36781 catcacagta taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa
    36841 gtcttttgta aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc
    36901 acgatacata ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt
    36961 tagcgcgcct gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat
    37021 ataagcagct tgcggcagcg tcacatcttt tgcatctaca ccaaagattc cttttgcagc
    37081 ttcctggact ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa
    37141 atacgtagca atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat
    37201 ctcgtttgct ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg
    37261 ctgtgtcaaa gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg
    37321 taaaacagct ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat
    37381 aattgcagtt ttcaataaag gtgaaatctt atcagatgaa atagtcgtgc gcatcagatc
    37441 tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat
    37501 gtttcctagt tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata
    37561 ggcaaaatag ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt
    37621 aataaaaaca gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc
    37681 tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac
    37741 taatggtttt atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt
    37801 tttggcagga atttcttttt taaaagagtt aaaacgtgag aaaatgatct ttattataaa
    37861 agagttctta agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa
    37921 aaatcaatca ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt
    37981 gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga
    38041 aaatgctgtt aggagcagca gcagaagcag ccagttatgg atcttcgact tttatgatct
    38101 atacaggcgc ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg
    38161 gaaaagcgta catggcagaa aacggtctaa caaatatcgt cgttcatgcc ccttatatca
    38221 ttaatttggg gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag
    38281 aagagattgt tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc
    38341 atgtaggggc aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt
    38401 tgacaaaaga acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa
    38461 tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaatta aatgaaaaat
    38521 tatccgttac ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact
    38581 tttcaggtgt aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc
    38641 atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt
    38701 ttggaacaat cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata
    38761 tttcgaaaat cttggaaact ccctatgtcg gcaaagataa aaagaaagct tacgcaccat
    38821 atggaaaaga aatcgccatg ttcaaagcac aaatattcga tccagaagtg tttcctgagt
    38881 tattagcaga agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag
    38941 gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag tactttttgc agtctttttc
    39001 atcatgcatc ttctcccccc tctttttaaa tttagacaaa caaggtatac tgttagaaaa
    39061 gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat
    39121 gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct
    39181 tggcatagcc ctattattta caattattcg tttgtattta tatagtaaag caatctatgg
    39241 aacagacatg tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt
    39301 ggacggtaaa tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag
    39361 cttattttta gtacttgcac ataaactgca tctttcttat gctgttttgt tcggcatgtt
    39421 cagtattttt gccagtatat tggttgcact cgcgtttaaa ccgatcgtga agaacaagta
    39481 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgaata
    39541 ttccactcga acatatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg
    39601 tttactagct atttattacc gcaaatacgg ttctagcaga aaattgctgc catggttttt
    39661 agggctttct gtcgcttttc ctttcttttg gttcattcgc gaagattcta tctggcttct
    39721 tccttttact gtagtagctt tgggtatcag tttcgtccag atcttgctag ggcaagactt
    39781 ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt cttctctcga aacgggaatg
    39841 gctgaaactt tcattgtttt tacttcctat agttggcttt tccatcgtct atcagtggat
    39901 cgaatataaa aatgagacaa catacggtat tgcaaccatc aatgaccgtt ccagtggaga
    39961 atttggacgt ttgatgaaac aactgatccg aatagatgat gaaacgaact tgaataaaga
    40021 aaacagtgag atatgggtct cacatgctgc actaaataaa gcattagccg catctcctac
    40081 attccatact gtttctaccc gtattgacga attatatacg actcacgcgt ggactcaagg
    40141 tggaaaagta aaagaattac ccggagatat catcttttgg gcattgcgag atgtggtaag
    40201 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    40261 ccaagaatta gaagaagcct atcacaaggg aaccatcaaa aagaaaaaag agttctatct
    40321 gatgggatca ggagatggaa agacgatgag tgatctgcca atcgtattcg atttccttca
    40381 atcaggatac gcttatgcat ggcgatataa agatttccat caaggaggaa attatagttt
    40441 cggaacgcaa gaacaagtca aacaggctca agaattatta aaccttccgc taatggagaa
    40501 ttggatcgat agtaatgaac cgagaacacc taatttccaa ttaacaaaat cagcaaaagt
    40561 cgcgaatgtc atcataaaaa tttaccaagt tctggctatt cccgtgctga ttgcttcaat
    40621 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    40681 tagttcctct ttgattgtgg tcaccggatt aatactgaca caattggttt ttcttttcgg
    40741 tgtctcgtgg ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac
    40801 tggcgcaggc gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa
    40861 attaaatctg aaaaaggctt tatcttctaa ataaaaagac ccttggtagc tagattttta
    40921 acctaactat caagggtctt tgtgtggaaa aatcagtttc tattcgattt cttctaccgt
    40981 ttcctgcaaa cgctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc
    41041 caacaggaga aggacaagaa caatgattac ataagcgatg tgatcagcca atgctagttg
    41101 agcagaagtg gcttgttcac caaagaaact ttcgatctgt cctggtaatc ctcgtaagtt
    41161 caagtaagtt agactaatca cagagaacca gccaagaagt tttacgatga atgaattttt
    41221 aaatcggcta cccatctccg cttggctgtt ggtcatcatc agtaaaggga tcatcgaaaa
    41281 cggcaacgca aaagccaaga acacctggga ttcattcatc aagttattca aagcggtatg
    41341 ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga
    41401 aagcaatcgt gtgaccagtc ttcttagcca aatcgggata cgcatatgga tgaagccttc
    41461 catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag
    41521 tgctacagca aataaagtgg ataagatacc agattttgct acagcaatca agataccatt
    41581 gctcatagct gatggatcag ataaggcttc atacagacca aaaaaggaag gatcttggac
    41641 agaaccggtt ttgaagacag cgacacccat gatcaatagc agtgcattga ctagaaaagc
    41701 catcgacaat tggatattag agtcccaagt agaaaaacgt acagcttgtg ctacatcttc
    41761 ttcgtcttgg tgatcaatct tccgagtttg agaaactgct gaatgaagat ataagttatg
    41821 cggcatgact gttgcaccaa taatccctaa agctccagtc agcggagttt cgccgccgat
    41881 gaccggtgag tctgagaatg tatgactgtt agggataaag ccagcaaata aactgtgcca
    41941 atctgggtta gacaagccaa cttgatagac aaaaacgaat aaaatgacga aaattaagca
    42001 gacaaccagt gcttcgatct tacgaaaccc gacttttgtc agtagtaaga gaacgaaaac
    42061 atctaataca gtaataaaga cggcaatcac caatggaata tcaaaaagta agtagagggc
    42121 gattgctgca ccaatgactt ctgcgatgtc tgttgccata atggctaatt cagtcaagat
    42181 ccataaaatg acacccaatg tcttacttgt gcgtgctcgg attgcttggg ccaaatccat
    42241 ttgggtcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa tcagactaga
    42301 catcaaaata actgacatca ataaatattg gaaattttgt ccaccagtga tcgaagtgga
    42361 ccagttacct ggatccatat aaccaactgc tactaatgca cctggtccag agtaagcaag
    42421 aagtgtttta aaaaaacttt tcccctttgg tacatcaact gttccattta cttcagctaa
    42481 agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttgt tggattgatt
    42541 attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc tcaaaaagaa
    42601 aaaattaaag aaaaatattc ggtatgccta atttattttc gtggcgcgtc ttgttagatg
    42661 acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat
    42721 acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata
    42781 gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt
    42841 gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga
    42901 aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tgggacaaaa
    42961 accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata
    43021 ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt attttgtaat
    43081 ttgttagact attcagatta ttcagcaaaa agacaagcac agacaatcct aggctcttga
    43141 taaagacaaa ggaggagtaa aatgtcgatt tttagaaagt taggctggtt ttttcgtcag
    43201 gaaaagaaac attatttaat tggggttttc tcgctattcg cagtggcctt ggtacagtta
    43261 gttcctccaa aagtgattgg gatcatcatt gatgagattg ccgataaaaa tttatcagta
    43321 aagatcattt tcgggtggat cttcctattg attatcgctg ctttcttaca atatcttttt
    43381 cgttatatct ggcggacgaa tatttgggga agtgcagctc ggttggagaa agaacttcgg
    43441 cagcagttat tcaaacattt tacaaaaatg gacaatctct tttaccaaaa atatcggact
    43501 ggggatttga tggctcatgc aacgaatgat ttgaatgcga tccagaatgt agcaggagct
    43561 ggtatcttga cctttgcaga ttcagtgatt acgggcggaa caacgatcat tgtgatgatt
    43621 ttgtttattg attggcgctt aacactgatt gcgctgattc ctctgccttt gctggccgtc
    43681 acttcacgag tgctggggtc gaaactccat gatgcttttc gtgattcgca agaagctttt
    43741 tctagtatca atgataagac acaagaaagt atcactggga tcaaagtcat taaaacattt
    43801 ggacaagaaa aagaagatct agcggatttt gaagaaaaga ttgaagatgc aattattaaa
    43861 aacaaacgag tcaattttct agactcctta tttgatccat tcattacctt gattatcggt
    43921 ctgtcttatg ttgcaacaat tattttaggt ggaaagtatg tcttggaagg cacgatctcg
    43981 atcgggcaga tggtctcatt tattagttat atcggtatgc ttgtttggcc gatgtttgcg
    44041 attggtcgat tgttcaacgt attggaaaga ggaaatgctt cttatgatcg cgtcaatgaa
    44101 cttttgcatg aaaaaacgca tatcgtggaa caactgggtg cagtgcagac cccggtaaaa
    44161 ggcgagctga atgtagctat ttcctctttt aattatccga aagaagaatc accagcatta
    44221 gaacaaatca agtttgcaat ccaagaagga gaaacattag gtattgttgg gaaaactggt
    44281 gcgggaaaaa caacgatcat caaattactg atgcgtgaat acgatgatta tcaagggaaa
    44341 atccaatttg gcggacgttc gatcaaagaa tattcattga atgctttatt aggatcaatc
    44401 ggctatgtgc ctcaggatca cttcttgttt tcgatgacca ttcgagataa tatccgtttt
    44461 tctaatccaa aactttcaga agaagaagta gagcatgcgg cttctttagc atttatcaac
    44521 aatgaaatca aggaattacc aaaaggttat gataccttag ttggagaacg tggagtctct
    44581 ttatctggcg gacagaaaca aaggatttcg attgctcgag cattgatcaa aaatcctgaa
    44641 ttattaatat tagatgatgc actttctgca gtagatgcca aaacagaaga agctatcttg
    44701 cataatttaa aagaaataag aaaagagaaa acaactatca ttgcagctca ccgtttaagt
    44761 agcgtgatgc atgcaaaaga aatcattgtt gtcgatcaag ggaaaatcat tgaacgagga
    44821 acccatcatg agctgcttgc actaggcggc tggtatgaac aaatgtggga gaaacagcaa
    44881 ttagaagcga agatagaagg aggcgaggcc tgatggatga aaattatcaa tcggaatgga
    44941 caaaatcgat tccacttaaa gaacagaaaa agatcatcaa acgactgatg aagttcgcca
    45001 aaccttttag aacaacgttc atcgttgcta tcttatttgc gtttgctctg tctgtgatca
    45061 atgtcctatt gccaaggatc atccaaactt ttatggatga tcatctggct aaacagtcgg
    45121 ctacgacaca agttattttg ttttttgctg gtatgtactt atttggagtc ctagtaaaaa
    45181 gtatcatttg gtttttccaa tggtatttgt attccatggc ttcattgaag acctatcagt
    45241 atattagggt aaaattattt gaaaagctgc atacactagg tatgcgttat tttgatcaga
    45301 caccggcagg atcaaccgtt tctcgagtga cgaatgacac agaaacgcta tttgaatttt
    45361 ggtacgtctt tttgatggta ttgactggga tctttgcagt cgtttcttca ttcattgcca
    45421 tgtttcaaat caatggagaa attgccttgt ggactttggt gttcttgccg attttaggaa
    45481 ttgtcatctg gtattaccag aaatttagtt ctcgtattta ccgaaatatg cgggaaaaat
    45541 taagtcagct gaatacaaaa ttgaatgaat cgatttccgg catgcgtatt atccagcagt
    45601 ttcgccaaga agctcgttta gctaaagaat ttgaacaagt gaacaatgag tatttagaca
    45661 cgcgctatgc gatgatcaaa acgaattcga ttctgttaag tccgatcatc aattttctat
    45721 atgcattggc gattgcgttg gcgttgactt tatttgggat cgatgcgttg cattctcccg
    45781 tagaagttgg actgatctat gcatttacga cttatgtcca gggatttttt aatccaatgt
    45841 ctcaaatgat ggactttttg agcatcttca cagatgggat ggttgctggt agtcgtatct
    45901 tgaagattat ggatacggaa gaatatgcac cacaacaaaa ccctggagca gatcaaatga
    45961 ttacttcagg taagattgag tttcgcaatg taagcttttc ttatgatgga gagcacaatg
    46021 tattaaacaa tatctctttt gttgctaatc cgggagaaac cgttgcatta gtgggacaca
    46081 ccggaagtgg gaaaagctcg atcatcaatg tgttgatgcg gttttacgaa ttttatgaag
    46141 gtcagatatt gattgatgat catgacattc gagaatatcc aatggaagag ttacgtaaga
    46201 aaatggggct ggtcttgcaa gatgctttct tgttttatgg ggatatcgca ggaaatattc
    46261 ggatgatgaa tccagctatc acagacgaac agatacgcca tgcggctgag tttgttcaag
    46321 caaacaaatt catcgatgaa ttaccaaaag gatatcactc aaaagtgatc gaacatggtg
    46381 ccagctattc tagtggacaa cgacagctga tcacttttgc gcgaacaatc gtgactgatc
    46441 ctaaaattct agtattggat gaagcaacag caaatatcga tactgaaaca gaaggattga
    46501 tccaagaagg attggcgaaa atgcgacaag gacggacaac gattgcgatt gcccatcggc
    46561 tgtcaacgat tcgagatgca gaattgattt tagttttaga caaaggtcat atagtagaac
    46621 gcgggaatca tgaaacactt ctagcgcaaa atggtttgta tgcagatatg tacaaactgc
    46681 aaagtagtgg tatataaaag atgttgtgac agtagcattt tgacctaagc actagagtga
    46741 aaataaaaca tagcttccca tatttttcat ctgtgagagt tagtgtcgta gttaccagat
    46801 ttaaaaaaga cgacgtcagc tagtcagaag tgcctaatag cccctaagaa agcgttgaca
    46861 aaatcgtttg ctttcgataa tataaagaaa acgatacaac ttggatagtg attgcctgat
    46921 gcaagctaaa cctgatgagt ctcccatggt ttcatggacg gctttttggg tttttctttt
    46981 tgttttggat acagccctat atatcatgga aaggagcaaa acatgctgat tgagaaagta
    47041 ctgaataaca acgtcgtaat aacaagggat gaaagagatc gggaaatgat tgtgatggga
    47101 aaaggattgg cattcaaaaa gaaggtgggt gacccgattc ttgactcatg catcgacaaa
    47161 aaatttcagt tgacaaatga cggactttca caacagttcc aagaattggc acaagcgatt
    47221 cctcttgagt acttagaaat ttcttgtgaa atcattgaat atacaaaaga ggctctgcaa
    47281 gtccatttaa atgattctgt gtatatttca ttgacggatc acttgtatac agctatcgaa
    47341 cgtcaaaaaa tggggatctt tgtgccaaat gtattgctgt atgatatcaa acaactgttt
    47401 cctaaagagt atgccattgg gaaaaaaaca gtgaagaaaa tctcggataa ataccacatc
    47461 aagttatcag aagacgaagc cggatttatc gcattgcatt tagtcaatgc acaaggcatt
    47521 caaagccagc aagacaatac ctacctgttt accacaacaa tacaggatat tttaaatatc
    47581 gtccgctatt actataagat tacatttgat gaagattcag tccacttttt tcgattcgtc
    47641 acacacctgc gtttttttct gacacgtgtc ctagatggag cgaaatcaga agatggagaa
    47701 gtttctgagg aattgttcca agttattcaa aaaaaatata aggatacgtt tggttgcgtt
    47761 gagaaaatag cagacttttt aaccggggct ttcgactacc agatgtccac ggatgaaaaa
    47821 atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa ataaataaaa
    47881 aaggaggaca aattaacaag aacgtatgaa ataagggtag tgacattcag ttggctaaac
    47941 cttccactaa ttaattggag ggaatagaaa tgggaaaata ccaggaatta gcagaaaaca
    48001 tcgtaaagaa tatcggagga aaagaaaata tccgtagctt gacacactgt gtgacgcgac
    48061 ttcgtttcaa actaaaggag gaaaataaag caaaggatga tgttttgaaa aagatggaag
    48121 ggatagtcac catcatgaaa agcggcgggc aatatcaagt tgtaatcggg aatcatgtgc
    48181 cagatgtcta tgcagaagta ctgacagtag ccgggatttc cgatgaagca gaagaagagg
    48241 tttcttcaag tagtaatctg ttcaatcgat taatcgacat attaagtggc tgtttccagc
    48301 cttttttagg tgctttggct gcagcgggaa tggtcaaagg attaaatgct ttgttcttgt
    48361 ttctcaaatt ttatactgaa ggatcgggta cgtacatcat gctgaatggg ataggggatg
    48421 caatttttta tttcatgcca gtgatcttag ggtacacagc agcgaaaaaa tttcatctga
    48481 atccgatggt ggggctcgtg attggtgcag cactttgtta tccgactgta caaggagcag
    48541 cactacaaag tggtttccct gatggcagtc ctgcgcctta tagcttgctg ggtcttccgg
    48601 cttatgaaac atttttgggt atcccatggg tgggcagcag ttatactagt agcgtcgtac
    48661 ctgttatctt cattatcgca tttgccggac aagtccaaaa aattgccaag aaatggattc
    48721 ctgcagtcgt ccagaccttt ttggtttctt tttctgtgtt attaatcgca cttcctgctg
    48781 gctttttgat tatcggtccg attgtcagta tactgacaga tctattaagc acgggattcc
    48841 aagcattgat ggacttttct ccagctgtgt atggtgcagt tctagggttt ctttggcaag
    48901 tactggtgat tttcggactt cattggagta ttatccctct tgcgattatg cagctagcgc
    48961 aaaatgggtg gagtcagatg ttagtaggcg tttatggcgc gagttttgca cagacagcag
    49021 tggtattggc gatgtatttc aagttcaagg atcaaaaaat gaaggcgctt tgcatgccag
    49081 ctgtgatttc aggaatcttc ggcgtaacag aaccggctat ttatggaatc accttaccga
    49141 agaagaagcc atttatttac tcgatgattg gtggtgctgt ttcaggaatc tatttgatgg
    49201 catctggaac aatcagctac ataatgggcg gattagggat ttttggggtg gtaaactaca
    49261 tcacggaaac agacgccagt ggtatgtttc aagcgtttat cggcattgct attgcttcgg
    49321 taatcgggtt ttcgctgact tttttctttt ggaaggatga agcaggtgaa acagatgcta
    49381 tacagaaaaa atcaggtaaa gaaattgtaa aaacaccgat acaaggacaa atcgctccac
    49441 taaatgcagc gaaggatgca gcttttgcac aaggaacact aggtcgagga atcttaatct
    49501 accctgaaaa aggagaagta cgtgcccctt ttgacggaac agttatgacc ctgttcccga
    49561 cgaaacatgc aatcggaatg gtttctgaaa cagggctgga attgctgatt catgtcggat
    49621 tggatactgt gcagttggaa gggaaatatt ttgaaagctt agtgcagcaa ggagaaagag
    49681 tgagacaagg cgatctgttg ctacgcttcg acaaaaagaa aatcgaaaag gaaggataca
    49741 gtttagaaac acctgtgatc gtcaccaatt acacggatta tttggatatt ttggaaaacc
    49801 aaggggagac ggtcgggcca gacgatgcac ttatcaccgc actgacctga acggttaggt
    49861 gaagaatgga ctaggcttaa ctattctttt tattccactt ctttattttc aaaattgaaa
    49921 ggctctttca tgatggctga gctgataggg cgtttatcga atttttgacc gacagaaatt
    49981 agatggacta aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga
    50041 agataacaaa ttgatgtatt gatcgtaaaa ttaccaattt ttgaatacag tcgctcgcgt
    50101 gtcgtaagtc gcaaagcggc tgtactcatt gcagatagcg ttgaatcacc gatactggtc
    50161 aaagctaaaa ttggaacatc attttgtttc aatatttctg cggtacgaag gatcacattg
    50221 ttttcacccg tataagaaat cagaatcccg cacatatcgc tcgtacaatt gtacgcttca
    50281 tagaatgatt caccaaaagt ggtgacaatt gtgacatcca ttttgatgcg cttcattttc
    50341 agcatgaaat cttgagaaat caaagtattc gagttactgg cgaagatcac gatttttttt
    50401 gcctggagca gtaaatattt agctttttgc agttggtcat gatgaatgag agaaagtgta
    50461 tcatgaatcg tcatctgttc taaagaagcc attttatttg cgattgtcat aataccgtct
    50521 gtggtggaaa aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt
    50581 tcagctaaaa aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca
    50641 cggatgagtg tagacgggtg gacataacat cgctcagcta tcgcttggat ggtcatttcg
    50701 ccaagtgctg gagaatcgct taagagaaac tggatcactt cattttctga aggagaaaag
    50761 gagatttctt tgattttttc acgtaataac atgtagatac cttcttctta tttttttacg
    50821 taaattataa cttttttgaa aatgttttgt aaacggattt atactatata acttaaaata
    50881 ggcttttttt gacaagatat ttacaaaaat ccactagaaa acagtaactt ttgtaaatgt
    50941 tactggaaag aaacggattt ctattgtttg ttttgtttac aaaaaagata atgaaagtgt
    51001 aaaaaagtaa aggagtgaga acatggcaaa aggaatcaaa atcgctacga ttggtggagg
    51061 cagcagttac acccctgagt tgatggaagg atttatcaaa agatatgagg aattaccgat
    51121 acgagaaatc tggctcgtag atgtagaaga aggaaaagaa aagcttgaga tcgttagtca
    51181 gatgtctcag cgtatgtggg atgcttctcc atatgaagtc aagatctatg caactctgga
    51241 tcgggaagaa gcattgaaag atgcggattt tgttacgact cagtttcgtg tcggtcaatt
    51301 agatgcacga atcaaagacg agcggatccc tagttattat gggatgattg ggcaagaaac
    51361 aaatggtgct ggcgggatat tcaaagcttt tcgtaccgtt ccagtcgttt tggcaatcgt
    51421 tgaggacatg aagcgtttat gtccaaatgc ttggctgatc aatttcgcga acccaagtgg
    51481 gatggtgaca gaagcagtta tcagatatgg caaatgggat aaagtgattg gattatgcaa
    51541 tgtgccagtg atggcgatga tgacagaacc agcaatgctt ggcaagaaaa aagaagagct
    51601 gatctacaag tttgccggtc taaatcattt ccattggcac aaagtagcag ataataaagg
    51661 aaatgaccgc acggcagaat tgattgatcg tttatacgat gaagataatg gattaccgaa
    51721 aaatatcttt gatatcccgt ttttcaaaga acaattaaaa caaatgaaca tgatcccttg
    51781 tggctaccac cgctattact atcgtgaaga agaaatgctg gaacatgctt tagaagaata
    51841 tcaaacgatt ggtacaagag cgcagcaagt gaaagaaaca gaagcagagc tatttgaatt
    51901 atacaaagat ccagaattag accataaacc tgaacaattg caaaaacgag gtggcgctta
    51961 ttattcagac gctgcctgtg aaacgattgc ttcaatctat ggaaataaag aaacacagat
    52021 cgttgtatct acaaagaatg atggagctgt tcctgacctg tcggcagatt gtgttgtaga
    52081 agtcactgcc tatgttggcg cacaaggagc gagaaatgta gcgtttggtt ctcttcctgc
    52141 tgctgaaaga ggctggttgc aagtaatgaa gaatatggaa ttgctgacga tcgaagcagc
    52201 tgtgactggt gattatggga cagctttaca ggcgtttacg atcaatccac ttatacgatc
    52261 aggaaagaca gcaaaacgga tcatggatga gttgtttatt gcacaccagc agtatttacc
    52321 gcaattcaag gaaacaatca gccgattaaa agaagaagga atcgaagttc aagatgaact
    52381 ggcaagagaa ttatcaggta atacacatat aatggaggaa gtataatggg gttcagaaaa
    52441 gattttttat ggggcggtgc tacagcagcc aatcaatgtg agggcgctta tgatgaaggt
    52501 ggacgcggac tagcaaacgt agatgttgtg ccaatcggaa aagaccgttt tcctgttatc
    52561 accggaaaga aaaaaatgtt tgaatttgat gaggatcatt tttatccagc aaaaattgca
    52621 atcgatatgt atcatcgtta taaagaagat attgctttgt ttgcagaaat ggggttcaag
    52681 acttatcgaa tgtctatcgc ctggagtcgt atttttccaa tgggggatga gaaagagccg
    52741 aatgaagctg gcttgaaatt ttatgaggat gtctttaaag aatgccaaaa gcacggaatt
    52801 gagccacttg tgacgattac gcattttgat tgtccaatgc atttgatcaa agaatatggt
    52861 gcgtggcgtt ccagagaact cgttggtttt tatgaaaatc tgtgtcgagt catcttcaat
    52921 cgctataaag gacttgtcaa atattggctg acattcaatg agatcaatat gatccttcat
    52981 gcaccattta tgggcgcagg gctgtatttt gaagaaggag aaaatgaaga acaaatcaaa
    53041 tatcaagcgg cacatcatga gttggttgca agtgccatcg caacgaagat tgctcatgaa
    53101 gtagatccgg agaacaaagt aggctgtatg ctggcagcag gagcatatta tccatacact
    53161 tgcaatcctg aagatgtttg ggcttctaga aaatcagatc gagaaaatta tttcttcatt
    53221 gatgtccagt cacgaggaga atatcccgct tatgcattga aagaaatgga gcgaaatggc
    53281 attcagatcg aaatggaacc aggggatgcc gcattgctga aagaacatac tgttgatttt
    53341 atctcttttt cttattattc ttcacgagtt tcgactaccg acaacgaact cttggaacag
    53401 actgcaggaa atatctttgc ttctgtcaaa aatccatatc tgcaagcaag tgaatggggg
    53461 tggcaaattg atccattagg cttgagaata acgatgaatg atatttatga tcgttatcaa
    53521 aaaccattat ttattgtaga aaacggctta ggtgcagtcg atacgccaga tgaaaatggt
    53581 tatgtggaag atgattaccg aatcgattac ctggcagcgc atatccaggc gatgaaggat
    53641 gcagtagaac tagacggagt cgatctatta ggctatacga cttggggatg tatcgattta
    53701 gtatcagctg gtaccggtga gatgaagaaa aggtacggat tcatttatgt cgatcgtgat
    53761 aaccaaggaa atggcacatt agcccgttca aagaaaaaat cattcaactg gtataaaaaa
    53821 gtaattgatt caaatggaga aaatctttcg aacaattgat caatgattta taaaagtcta
    53881 ttgtttttca gcggattttt cgagtataat aaagctattc gagaaacgca agcagaacaa
    53941 tagacttttt ttcgcattgg ctgtaaagta tgcttacttg attaagtaaa ggagaatcta
    54001 catatgtatg atgtaatcgt tattggcgca ggtcctgctg ggatgactgc tgctttgtat
    54061 gcttcacgtt caaacttatc cgtattgatg atcgaacaag gtgcccctgg cggccaaatg
    54121 aacaatacgg cagaaatcga aaattatcct ggattcgatt cgatcatggg ccccgatcta
    54181 gcttataaga tgtatgaaag tgtctcaaga tttgggacag aaaatgctta tggaatcgtt
    54241 caagatatca aggatcacgg cagccataaa gaagtcatct gtgaagataa aagttacgaa
    54301 ggaaaaacag tcattatcgc aacaggctgt atccaccgta aattaggcgt acctggcgag
    54361 gaagaatttg ctggacgagg cgtttcttac tgtgcagtct gtgatggtgc gtttttccgt
    54421 aacaaacgtc tagtagtcat cgggggtggg gactcagcag tagaagaagc catctatttg
    54481 acacaatttg cttctgaagt agtgatcgtc caccgtagag atgaattacg tgcacaaaag
    54541 atcatccaag accgtgcatt tgcaaatgaa aaaatttcct ttatgtggga tacagtcgtt
    54601 gacgaaatcg ttggaaacga aatggtagtg acaggtgtga aagcacgtaa tgtcaaaacc
    54661 ggcaatgaat ttgatttagc agcagacgga gtattcatct atgttggact ggatccatta
    54721 actgcacctt ttagagaagc aggtttgacg aacgaagctg gctggattcc aacagacaac
    54781 gaaatgaaaa cgaatatgcc aggcgttttt gccatcggag atgttcgtga aaaagatttg
    54841 cgacaaatca ctacagctgt tggtgacggc ggtattgctg gacaacaagt gtataaatac
    54901 ttggagtctt tagaagaaac aacagcataa gtttaaaaag agtgaaagaa cggtttcaat
    54961 tgttctttta ctcttttttt tcgcgtgaaa tccactgaat ccgatttatg tgaatacttg
    55021 caatcatgct tgataaaaga atcctttctt gatttaagcg ttttttggtc ctcatacttt
    55081 catatctaat aagtgaatgc tatacttatt aagaaccata tttcagaaag aggtgttatt
    55141 ttcatgtctt gggaacaagt gtatgaacaa tgggtacatg aagaaaattt agaagaaaac
    55201 ttaaaaaaac aattaaatga tctgggtgaa gatcctgaaa aacttgaaga tgctttttat
    55261 gcaccgttgg aattcggtac tgcaggcatg cggggaatct taggacctgg gatcaatcga
    55321 atgaacatct atacggtaag acaagcaaca gaaggactag cacgtttcat ggatacacaa
    55381 ggtgaagaaa caaaacgccg aggtgtcgct atcgcttatg actcacgcca tcagtcacca
    55441 gaatttgcaa tggaagcagc caaaacactg gcacatcaca atatcccgtc ttatgtattc
    55501 gaaagtctgc gtccaacacc agagctttct tttgcagttc gtcatttgaa gacgtttgcg
    55561 ggaatcatgg taaccgcatc tcacaaccca gcggcttaca atggatataa agtctacgga
    55621 gaagatggcg gacaaatgcc tcctgcagac gcagatgcat tgacgaaata tgtccgtgaa
    55681 gtcagcaatc cgctaaaagt cgaagtatta tctgacgaag aagctaaaca tagcgggttg
    55741 atcacgatca tcggagaaga agtggacgct gcttatctag aagaaatcaa agcagtgacg
    55801 attgatcgtg aattggttga aacaatgggg aaagatttga aacttgtgta cacgccactt
    55861 catggaacag ggaaaatgtt gggcgaacgt gcgttgaaac aagctggatt cgaacaattc
    55921 gtactagttc ccgaacaagc tgtggcagat ccagatttca ctacggtcaa atcgccaaat
    55981 ccagaagaac attcagcttt tgaatatgct atacgacttg gagaaaaaga gggagctgac
    56041 ttgctgattg caacagatcc tgatgcagac cgtttaggcg cagcggttcg tttgccagat
    56101 ggttcttatg aagtattgac tgggaaccaa atcggagcat tgtttgctca atatattttg
    56161 gaagcacatc aatcaagagg cactttacca gaaaacgcag cggtattgaa atcaatcgtt
    56221 tccagcgaat tgcctacagc aattgcggaa cactacggtg ctactatgtt caatgtgttg
    56281 acagggttta aattcattgc tgaaaaaatc caacaatttg aagaagacca ttcacatact
    56341 tttatgtttg gattcgaaga aagttatggt tatttagtta aaccttttgt tcgcgataaa
    56401 gacgcgatcc aagcattgct tttattagca gaagtagcag cttactataa aaaacaaggc
    56461 aaaacgttgt atgacggctt gcaaacgatt tttgaaacct atggctattt cgcagaaaaa
    56521 acgatttctg tgacaatgag cggacaagaa ggttctgcga aaatctctgc tttgatggaa
    56581 aaattccgta aagaagcacc gacagaattt gccggagcaa aagttattca aacagaagac
    56641 tttaaagaat tgacgagaac agaccgtgaa ggaaacgtag aaaaactaac aacaccgcca
    56701 tccgacgtat tgaagtatac attggaagaa aatggctgga tcgctatccg cccttcagga
    56761 actgagccga aaatcaagtt ttatattggt gtaaaaggaa gctctacaga agacgcagcc
    56821 gaaaaagtag cctcttatga agcgattatc aaaaagatga cagaagaata aaaaacttga
    56881 gactgatgga aaaagtggta aactttagaa gtcagtcttt tttcaagttg gaggtgaatg
    56941 gattgccaga cacgcaaaaa tcaaaagtag agaaagtcag tcaattattc aaaatgttga
    57001 gtgaccccac tcgtcttaaa attctgctat atctgaaaga cggggaacaa aatgtgacag
    57061 caatcaccca agcagtggaa atggagcagt ctgctgtatc gcatcaacta cgtttattaa
    57121 gagaaaatca tgtcgtcaag tctcatcgcg agggaaaagc gattttgtat agtcttgatg
    57181 attctcatgt actggatatt ttgaaccaga cactcaaaca tgtggaacat caataacgat
    57241 aggtgcaaat agataaacac tggagtgtga caacgaaatt tcttcgttgt cacactccag
    57301 tgttttttaa gaaaaagaca aaaatgatta atcgcttttt tctgttgttt cagttccata
    57361 ataactatga tcttctaggt ctaaagcatt caaaataagc gaagccgttt cttcaataga
    57421 taaagatgcc acattgatca ctacacatcc tagtttttca taaagatcat tcgcaaaatc
    57481 aagttcagct ctaatttttt cgatatcgga ataagcagta tcagggttta atccgtaaga
    57541 gcgcatccgt tctttacgga tattatttaa aatatctggg ttattcgtta gtcccacgat
    57601 ttttttagga tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtaa
    57661 attggcaact tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttcg
    57721 ggatacgcct agcaaaacga catctgcttc taaaaaacca cgaggatctt tcccatcatc
    57781 gtattttaca gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg
    57841 gagggcacca ggttgccgaa ttggcttgat accagttaaa cgttcaattt ctgatatcgg
    57901 tggcgtcatc acatcaaaac taaataattg atgttcttca caaaaagaac gtgttatctc
    57961 gatcagttct tgattgatta atgtatgcag gacaagtgct ttttcttcta atgcatcttg
    58021 caatgcctgc cttaatgttt tttcatctgt cacgaacgtt ctgcggaata ggttgaactc
    58081 aacggtagga tactgagcca tagtagcttg cgctagtttt gtcgcagttt ctccagcaga
    58141 gtcggagatg acaaaaaaag taacgatttc tgtagaatcg gaagacatgg ataaaacctc
    58201 acttgcattt tttttatgtt tattatacca tactctagaa tgcgtacttc gtaatcattt
    58261 tgatttggag gaataagtca tgaaaaaaca gacattgatc gaacaatcat tagaaacatt
    58321 gaaaaataac ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa
    58381 gcgtaaccgc tacgtatctg ctaaagaagt ttttgactat atgaatcagg aattcaaagg
    58441 ggtaagttat gatacgattt accggaatct ccatgatttt gcagagatcg atttactgga
    58501 agaaaccgat ttaaatggag aaatgaagtt tcgttttcat tgctgtcagg ggaaaatcgg
    58561 ccatcaccat cattttatct gtacagtgtg cggaacaacg aaagaaatcc atatgtgtcc
    58621 gatgaattat ttcgaagaac agctaggtgg atgcacgatc gaagggcata gattcgagat
    58681 tttcggaaga tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta
    58741 aaaaaaagca agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag
    58801 tcttgttttt tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag
    58861 ggaattaaca tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt
    58921 cgcttcaaac gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc
    58981 tacgaaaaac caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa
    59041 ttctagttct gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg
    59101 acatgaaagc agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca
    59161 agtcatctat acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaac
    59221 taacggtgtt gtctcgtgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag
    59281 caggacgtga cgatttagct gaaaaagtaa aaagtgaaat tgtaatagtc gaaaaatata
    59341 tgccggaaca actttccgac gaagaaattc gacaacttgt tcaagaagca atcactcaaa
    59401 ctggtgcttc atcaatgaaa gaatttggca aagttatggg tgcaattatg cctaaagtca
    59461 aaggaaaagc agatggaaac caagtgaatg cgattgtgaa agaactatta caatcttaat
    59521 tttatcattt aaactgcgga cagtgattat ccgcagtttt ttgtgttcgt aaaaaaagaa
    59581 ctaaaaaaca gctagaagaa agaatcttat tctttcttct agctgttttt ttcctgttta
    59641 atatctctta tgttaaattg tgtttgattt ggttattctt ctggtaactc acttgcttct
    59701 tgtttatatc ctgatacgcg atatacataa taaaagagca agctgccagc gtaaccaagc
    59761 aacgttggga cgacccagcc aagacctaat gcagcaaaag gaacataatg atgataaaat
    59821 cctagtaagc ttgctacgac agtttgctga ctcactaatg caggcgaatt tgccaacata
    59881 tccaatacgg ctggaacggc agaaaagaac atcgttactt gatagacaaa tggactcttt
    59941 tggaagaatt ttgctgttaa ggaaagtaag attaacgcaa gtgacaatgg atacaaaagc
    60001 atcaatactg gtaaagacca ttggatgata ttatccaatc cggcattcgc aacgacaaaa
    60061 gagacaaaag tcgttaaccg cagccatgtc atataactga ctttagggaa cagtttatgg
    60121 aaatcttgtg caaaagaaac aacgagtccc atagcagtag tgaagacacc taatgtgacc
    60181 attaccccta agaagctagc accgaagtta cctaaataaa aattagtgat ctgagaaagt
    60241 gcagttccgc cattttctga taaagacatc ttattcaaac tcatcgcacc gagaaggacc
    60301 agaccaaaat aaatcatgac ttctaacgca acgctcaggc taccagcttt tgctgttaat
    60361 tctgtgactt ttttatcttt aaaaccaaga tcacgaactg catggacaaa agtgacactt
    60421 aaagcaagta aagctactgc atcaaccgtg ttgtatcctt ctaaaacgcc atttgtcaaa
    60481 gcgttcgtct gataagaatc tgcagcagat tggtttagat tccccatggg ttggaataaa
    60541 gaaacgacaa aaatgatagc taataatgca agaaacaagg agtttaaata ctttccgaca
    60601 tatttgacca aacgtgttgg atgtacagta aggaaataag caagtaaaaa gaaaagagca
    60661 gagaataaaa acatacctaa tgaatcatat ttttcaggca aaaagggttt agcagccatt
    60721 tcaaaagcag ttgctgctgt ccgcggtgta ccaaagaatg gtccgatcgt taaatgaaca
    60781 aggatcaaga acaaggcagc ataccatttt ccgacaggtt tagctaagtc atacagacca
    60841 tcactttttg tcactactac agctaagata gctaataatg ggaaaagagc gcctgagatg
    60901 gcaaatccta caccagcgct gatccagttt gatccagcca tttgacctaa gtgtacagga
    60961 aagatcagat tacctgaccc gaagaacatc ccgaagataa gtgaactgac catcaataat
    61021 tgcttccatg aataagtttt tgtttcttcc ataaattttt cctccaataa ttatcatttt
    61081 gagtaacaaa ataaacgttg cagaaactag aaataccact aatatcagat tccatgaaaa
    61141 ccaccacctt aaaatataaa aaaagccctt ttccacttgg gaaaagggct ctagattcta
    61201 gaacggtacc accttttttc gcttaaaaaa gcttcttcat tcacggacgc ttttcgataa
    61261 agttatccgc tagcctgata atggaggcag ccacaatgat ctacgttttc gacattgact
    61321 cggaaatgat gttcgtctat tcgatttatt gtcatttctc acttacatga ctcactggga
    61381 ataaatcact gaatagccta ctgttttcct cgacgctttg ctgtatattt atttgttgac
    61441 gttataatac acctgttttt tttgaaatgc aagaatattt caaatatttt gttttctttt
    61501 tttgaaacaa gaaaaatacc cagagaatac ttgctaagtt agacggaata cttgtcctct
    61561 atcttatgca tagcttacat gatcagcttt ttttattcaa acgagacctt tagagaatgg
    61621 aagcggctag ttttttaacg tttcttggta cttatggtta agcaactctt ttagaacctt
    61681 ttcttatttg ctatatagtt tcttctgagt atggtaaaat aggaaagaat gaatcgaaaa
    61741 aaaggggatg tctatttttg acggaagagt ctagttcttt agagattaga ttgacggctg
    61801 ctgatgatgt cagcatgctt ttaggtgcac acgacaagca tataaaactg attgaggaaa
    61861 caacggaaac aaccatccat acacgtggag aaatcattca gatcattggt gagcaatccc
    61921 agatttcctt ggctgcctca gtcattcgga cattacagga attgatcaaa cgaggaatcc
    61981 acgtaagtac gcccgatgtt gtgacggcat tgaaaatggg gcaaaaaggt acattagatt
    62041 attttgttga gatgtatgag gaagagatcg tcaaagaccg taatggaaaa cctatacggg
    62101 taaaaaacag cggccaaaag aaatatgtcg aatcggtagc caaacatgat atcgtatttg
    62161 gcgtaggacc agccggtaca ggaaaaactt ttcttgcagt agtattagca atcgctgcct
    62221 tgaaaaaagg tgaagtgcaa aaaatcattt tgacgcggcc tgctgtggaa gcaggtgaaa
    62281 atctaggctt tcttcccggg gatctaaaag aaaaagtgga tccttatctg cggcctgttt
    62341 atgatgcttt ataccaaatt tttggattgg atcatacgaa ccgattgatg gaacgaggcg
    62401 taatcgaaat cgcaccttta gcttacatgc gggggcgaac attggatgat gcattcgtta
    62461 tcttggacga agcacaaaac acgacagtcg ctcagatgaa aatgttcttg acccgtctcg
    62521 gttatcaatc aaaaatgatt gtcaatgggg atacaagtca gatagacttg cctcgaggga
    62581 cgacaagtgg tcttgttcat gcagaacgaa cattaaagca aatcaagaaa atcgattttg
    62641 ttaattttga agcaagtgac gttgttcggc accctgtagt tgccgaaatc atcaaagctt
    62701 atgaaaaggc agatctccat caagaataag ctttgcttag gaggataagc acatgttgaa
    62761 taaaacactg cagaaactac aagaaaaact tggaaaggca gcattgcctt tcctgctgtt
    62821 cttgtttttt atcattatct gcggcgttac atttagtagt gtcaaacaaa aggccaatga
    62881 tttcagagaa ggccaagtag cagaagaaag tatccgtgcg aacaaaacga tcgagaacac
    62941 ggaagaaacc gaacaaaaaa gaaaactggc agcagaagca gtcactccag aatacactta
    63001 ccaaaaagat cttgcagatg accagaacaa tcggatcaag caactatttg aattgatcga
    63061 taagacgaat gatgccatca acaaatctta tgacgaaaag attgataagg caaaggaaga
    63121 tgaaaatatc ccaaagccta ctacagaaga gcggatcgcc gcgctgaaga aaaattttga
    63181 aaatgtgaat gcagaaaatg ttgtgtttta tcaaaaacta ccgaacaatt tttattcgac
    63241 gatatttgaa atgactgaaa ctgagattca tactgtgagg gatgagagtc tgaagctcat
    63301 tgacgaacag atgagcaaac aagttcgtga aagtgaattg gaagctttta aacaagaagc
    63361 agaagagcaa atccaatatt tgaatgtcac accagcacaa caacaaatga ttcgctactt
    63421 agtggatcaa ggtatcgtag tgaatgacgt attgaatgag aaaaagacag aagaattgaa
    63481 acaatccgca cgagaagctg tacaaccagt gatgatcttc caaggagaaa tcattgttcg
    63541 tgaaggtaat caaatcgatg cttcagctat gaaaaaattg gaattactag gactgactaa
    63601 tcaaacaact tcgatttttc cgctaattgc catggtttta gctgttttgc ttcaaatcgg
    63661 cgtactgatt tataattcac ttcaattcca aaatgcagga gaaagaacaa aatacgtgct
    63721 tttctatgtg acagctatgt cagtcagcat agtgctgatg aaatttttcc aattattcca
    63781 aacggaacaa gctacttata tcccgctgtt ttatcccgct gcttttgcac cacttgtttt
    63841 aagctttttc ttgaatcgaa gagcagggat catggctggt attttccaag cagtatccgc
    63901 acttttcatt ttttatgatt cgattggcac gaactttcta acgattatct tgatgtctta
    63961 tgttttctct gggctcatgg caacgattgt cagacgcaaa cggatctcag aacaaggatt
    64021 atctgctgcc atgtgggtca ttatttttcc tgtgatgatg gatgtcattt tgatcgtcta
    64081 tcaaggaatg agctttggtg atgcaacgac ttggttaatg ctcgtctgcg ggttcactgg
    64141 tgcactcttc tcattcttgc tgacgatcgg tctacaccca tatattgaat tgatggtcaa
    64201 tgatgacagt gtaattgtat taaatgaact cagcaatcca aatcatcctt tattgaaaca
    64261 gttgctggaa gaagcgccag gtacttatca tcatagtatg atggtagcta atctaagtgc
    64321 taacgctgta gcagaaatcg gcggacgttc tttgctaact cgagtagctt gctattatca
    64381 tgatatcggc aagatcaaac atgctagctt tttcgttgaa aacttgcctt caggagcgga
    64441 aaacccgcac aatttcttat tgccagaaga cagtaaacat attatctttg gacatgtgac
    64501 agatggagcc aaaatattgg aagattataa tatgccgcaa atggtgatcg atatttgcaa
    64561 acagcatcat ggaaccacgc taatgcgtta tttctacatc aaagcgaaag aacgcaatcc
    64621 ggaagtaaca gaagagcaat tccgttatcc tggaccaaaa ccgcaaagtc gtgaagcagg
    64681 gatcgtcaat attgctgaca gttgtgaggc tgcagtacgg gcaatggatc atccgacgag
    64741 cgaaaaaatc gaaagttttg tccataatct tatcgaagag cgtatttccg atggtcaatt
    64801 agacgatagt ggactgacct tgaaagaaat ccgaaaagtg gaaaaatcat taatcagcgg
    64861 tttaagtagc accttccatt ctcggatcaa atatcctaaa atgaaaacag aagttgagaa
    64921 gataaaggaa gaacaagaag aatagaaaga agcgatgtat aatggatatt acctttatgg
    64981 atgaaacaaa ccaagtctca gaagagaaaa tgaaagaaat cgatgacttg ctgcaatttg
    65041 cagcatctta tttaaagctg ccagaggaaa cagaaatgtc tgttactttc atggacaatg
    65101 cggcaatcca agtaatcaat cgtgattacc gtggaaaaga tgcaccgaca gacgtcatca
    65161 gttttgctct ggaagaagaa ggcgaagacg aaatcccagt gatttttgaa gatggagaga
    65221 atccattacc tcgtgtacta ggagacatca tgatctctat tgaacgtgct gctgaacaag
    65281 cagaagctta cggccattca tatgatcggg aacttggctt tttagcggta catggattct
    65341 tgcatatcaa cgggtatgac catatgacac cagaagatga gaaagaaatg tttggtttac
    65401 agaaagagat cttggatgcc tatggactca aaagataaaa acgtagagaa aaacaaacat
    65461 ttcctgatgt ctgtggaatt tgctgtccaa ggagtcaaaa ccgtttttga ggaagaacgc
    65521 aatatgagga aacatgttgc gttcggtatc cttgctttat ttatgggcgt gattttccag
    65581 cttgatcgga tagagtggtt atggctgctt ttagctgttt ttctcgtttg gatagtagag
    65641 atcatcaata ctgtttttga aaatgtagtg gacatgttta cggattttca ttttcatccg
    65701 attgggaaaa aaatcaaaga tatggcagct ggagcagttt tagtgaccgc ttgttttgct
    65761 gtcatcattg ggttgatttt atttgtccct aaaatatatc atttgttgtt taattaaaaa
    65821 ggagaacgta tatgccagaa cataaatcag ggtttgtcgc aatcgtcggc cgcccgaatg
    65881 taggaaaatc cacattattg aaccgaatcg tgggtcaaaa aattgccatt atgagtgaca
    65941 aggcacagac aactagaaat aaaatccaag gagtatatac tacaccagaa gctcagctga
    66001 tttttatcga tacaccagga atccataaac caaagcatcg tctaggagat tttatggtag
    66061 aagcagctta cagtgcgttg aaagaagtgg atgcagtctt atttatgatc agtgctgatc
    66121 aaaaacgtgg ccggggagac gattttatta ttgaacgtct aaaaaacgtg cattctcctg
    66181 tttatctgat tatcaataaa atcgataaga tccatccaga cgagttgctg ggaatcatcg
    66241 aagattattc gacgcagatg ccttttgcac aagtgattcc gatctctgct acagaaggaa
    66301 acaatgtcga acgcttaatg gatgtattag tcagtgaaat gccagagggc ccacaatatt
    66361 ttccagatga tcaagtgact gatcatcctg aatacttcat cgtttctgaa ttgatccgtg
    66421 agaaagtctt gtttttaaca cgagacgaag tgcctcattc tgtcgcagtc gttgttgatt
    66481 cgatgaagcg aaacgagaat gataaaatcc atatccaggc cacgatcatc gtcgaaagag
    66541 atagccaaaa aggaatcatt atcggcaaag gcggaaaaat gctgaagcaa gtaggaacga
    66601 aagcaagaaa ggatatcgaa aatcttttag gagacaaagt atttctagaa ctttgggtca
    66661 aagtccaaaa agactggcga gacaaaaaag tctatctgca agacttcggc taccgaaaag
    66721 acgagtactg atattctgga cttcttatag gttaggtata gaaataaact ggataaaaat
    66781 ccgtacgttg agaaaaacag cggaagatcg aaacataaat aatctggatc ggcagaatct
    66841 gcttttccag atttattttt taggagggag aatatgagcc agcttgcaga atcgaaaggc
    66901 ttgattttat ctagccgtaa ttacaaagaa aaagataaat tagtcaaaat ctttacggaa
    66961 tcttctggca aaatgatgtt ttatatcaaa ggaatccatc ggcaaaatca gccattagct
    67021 ccggctttgc tcccctttac cgaagcagtc tatatcggta attttcgaag tgaaggtttg
    67081 tcgtttttaa gcagtgtcaa agacgtccat gcgtttcgga acatccaaga agatatcttt
    67141 ttgagcggtt atggtacgta tttgctaaat ctagtagatg cagcaatcga agatcatcaa
    67201 tatgacccgc atttattcca atttacgcaa caagctctgc agcggatgga ccaaggagat
    67261 gatgcagaaa tcatcacgaa tatctttgaa gtgcaaatac tgcaacgctt cggaatcgcg
    67321 ccaatttgga cgcactgtgt cgtatgcggg gaaacaaaag gaaaatttga ctattcttcc
    67381 aaatatggcg gtgtgatatg cgagaaacac tggccaatgg acgaacaccg ttatcatgca
    67441 gatcctcgcg ctgtttattt cgttcgaatg ttttcggcta tttcttatga taagatcagt
    67501 ggaatccact tgaaagaaga aacgaagcag gcaatccgtg acctcattga ccagttgtat
    67561 gatgaatatg tcggtattca tctaaaaagc aaaaagttca ttgatcaaat gaagtcttgg
    67621 gcacacgtat taaagacgcc agagaaaaat atgcctgaaa aagagatacc tgaaaaagaa
    67681 gaataaatga aagatacaac aaaaaaatca aaactatttg acaaatacag ggacttccgt
    67741 ctattattaa ccgtagatta aatagatcgt tcactgagag gatctattat acaaaccaga
    67801 aaactgcaaa atgaatacgt acattgacca agatcagtaa aaaacacgtg gtttagcgag
    67861 ttcgggagag tggaagccgg acccaaacga tttttgaaga ggcacttgcg agtacgataa
    67921 atgaagctgc cgaatcttta ttcggaagta gggtggaacc gcgaaattat tcgtccctat
    67981 gtctgtaatc agacataggg actttttgtc gttttctttc ctgtattctt attgacagca
    68041 acgcttaaaa attgaaaggg gcaaaacaga tgagtaaaaa attgaccgtc caagatatga
    68101 ttttaacatt acaaaaattt tggtcatcaa acggctgcat gctgatgcaa gcttatgata
    68161 cagaaaaagg agcaggtacg atgagcccat atacattctt acgggcaatc ggaccggaac
    68221 catggaatgc tgcttatgta gagccatccc gccgaccagc tgacgggcgt tatggagaaa
    68281 acccaaaccg tttgtatcag catcatcaat ttcaagtcgt aatgaaacct tcacctgaaa
    68341 atatccaaga gctttattta gaaagcttgc gtcttttagg aattgatcca ttagaacacg
    68401 atattcgctt tgttgaagac aactgggaga acccatcgat gggttgtgcc ggtgtgggct
    68461 gggaagtatg gcttgatggg atggagatca cacaattcac atacttccag caagtcggag
    68521 gactaccttg taaaccagtt acttctgaga tcacttatgg actagaacgt cttgcttctt
    68581 atatccaaga agttgaaagt gtttatgatc tggaatgggc tgatggcgtg aaatatggtg
    68641 agatcttcaa acaaccagaa tatgaacatt ctaaatattc ttttgaagta agcgaccaag
    68701 acctgctact aagcaatttt gatcgttttg aaaaagaagc taaacgctgt atcgatgaaa
    68761 atcttgttca tccagcgtat gattatattt tgaaatgtag ccatacattc aatttattag
    68821 atgctcgcgg tgcagtatcc gtaacagagc gtgcaggtta tctttcccgt atccgcaaca
    68881 tggctcgtgc tgtagccaaa atctttgtgg cagaacgcga aaaattagga ttccctttat
    68941 tgaataaaaa cacagcaaat ccagaaaacg tgaaggaggc tgaataagat ggcaaaagac
    69001 ttattacttg aaatcgggct ggaagaaata cctgctcatg tagtcacacc aagcatgaaa
    69061 caattagaag aaaaaacagc aaaattttta gatgaaaacc aattgactta tgatacgatc
    69121 gaaacgtttt ctacaccgcg ccgcttagca atcaaagtaa caaacattcc agaacgtcaa
    69181 gcagacagcg aagaagaagt caaaggacca gccaaaaaaa ttgctttgga cgcagatgga
    69241 aactggacga aagcagctga aggatttgtc cgcggacaag gtctgacgac agatgctatc
    69301 acttttagag aattaaaagg cgtggaatat gtctatgtaa cgaaatacac tcacggcaaa
    69361 gaagcaaaag aagtattgac aggtttagca gatgtcatta aaagcctcac tttcccagta
    69421 acgatgcact gggcagaata cgattttgaa tatattcgcc ctattcactg gatcgtggca
    69481 ttgttagatg acgaaataat cccgtttgag atcttggatg tcaaaacaga cagagcttca
    69541 agaggtcatc gtttcttagg cgaagatgtg acatttacgc atgcaaaaca atacgaagaa
    69601 aaattgaatg aacaatttgt tattgcttca ccgcaaaaaa gaaaagaaat gatcatcaca
    69661 caagcaaacg aattagcaga aaaaaatcac tggatcattg atctagatga agatttacta
    69721 gaagaagtca acaatttagt cgaatatcca actgtctttt ctggcgactt cgaagaaaaa
    69781 tatctatctg ttcctgaaga agtactagtc acttctatga aagaacatca acgttatttc
    69841 gaagtacgga acgatcaagg tatgttgctt cctcatttta tttctgctcg taatggcgat
    69901 cgggtaaaat tggaaaatgt ggtacgaggc aatcaaaaag tattgactgc tcgtttagaa
    69961 gatgcggaat tcttttataa tgaagacaag aaagcttcaa tcgaccaata cgttgaaaaa
    70021 ttaaaaactg ttactttcca tgaaaaaatc ggtagcatct atgagaaaat gcaacgggta
    70081 caagtcatcg ctcgcatcat aggcgaacaa gtaggactat ctgaggaaga attgatggat
    70141 ctgcaacgcg ctgctgaaat ctataaattt gatctagtaa ccaacatggt aggagaattc
    70201 cctgaactac aagggatcat gggcgaaaaa tatgctctgc ttaaaggcga gaaaccagcc
    70261 gtagcaacag cgatccgtga acattacatg cctatatcca gtgaaggaga tttacctgag
    70321 actacagtag gttctgtttt agcgattgct gataaattag atagtatctt ttctttcttt
    70381 gcagttggga tgatcccaag tggctcaaac gatccatatg ctttgcgccg tcaagcatat
    70441 ggtgtcgttc gaatcatcga atcgaaaaaa tgggcctttc ctttgtctgt tctgcaagaa
    70501 acgattagtg aagttatcag taaagatacg gatcgttttg gcattggctt gtcagcggga
    70561 caacaacagg tgatcgactt tatcaaaggc cgtctgcgcc aattattgac aacgaagaat
    70621 atccgtcatg atgtcatcga agctgttttg aatgcagaac aaaaagatct gacaaaagta
    70681 tttgcagcag cacaattgtt caagcagcat cttgcagatg aagacttcaa accatcgatg
    70741 gaagctttga cacgtgtcgt gaacttagcg aaaaaagcgg agcttgaaaa acaatcagaa
    70801 gtagaccctg aactgttcga aaacgaagcg gagaaagaac tgcacaaagc agtagaggca
    70861 atgaaaacaa gctttgctga ccaaacgatc aacgaaaatt atgatcagct tgtaagcctt
    70921 cgcccactga tcgaaaatta ttttgatcag acgatggtca tggcagaaga accagcagta
    70981 cgtaagaacc gattaacgca attaagtcaa attgctgcca tggctttgtc actagcaagt
    71041 ttagatcaaa tcatcacaaa ataatttcta atatgtaaat aaaccagaga acaggcacaa
    71101 aataggctat ttctctggtt tatttgttat aaatataaat aactattatt aattcaatgt
    71161 taattttttt gttattttat tttttgtgta acttttaaaa acacctatga a
//
LOCUS       DAYYGY010000004        70243 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000004 DAYYGY010000000
VERSION     DAYYGY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.004"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..791
                     /locus_tag="ACHA52_000257"
     CDS             39..791
                     /locus_tag="ACHA52_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8939015.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            788..1702
                     /gene="pfkB"
                     /locus_tag="ACHA52_000258"
     CDS             788..1702
                     /gene="pfkB"
                     /locus_tag="ACHA52_000258"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8939016.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1720..3651
                     /locus_tag="ACHA52_000259"
     CDS             1720..3651
                     /locus_tag="ACHA52_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8939017.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFRGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3762..5321)
                     /locus_tag="ACHA52_000260"
     CDS             complement(3762..5321)
                     /locus_tag="ACHA52_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8939018.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5476..7710
                     /gene="pcrA"
                     /locus_tag="ACHA52_000261"
     CDS             5476..7710
                     /gene="pcrA"
                     /locus_tag="ACHA52_000261"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8939019.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7727..9763
                     /gene="ligA"
                     /locus_tag="ACHA52_000262"
     CDS             7727..9763
                     /gene="ligA"
                     /locus_tag="ACHA52_000262"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8939020.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9869..10174
                     /gene="gatC"
                     /locus_tag="ACHA52_000263"
     CDS             9869..10174
                     /gene="gatC"
                     /locus_tag="ACHA52_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8939021.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10174..11646
                     /gene="gatA"
                     /locus_tag="ACHA52_000264"
     CDS             10174..11646
                     /gene="gatA"
                     /locus_tag="ACHA52_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8939022.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11643..13073
                     /gene="gatB"
                     /locus_tag="ACHA52_000265"
     CDS             11643..13073
                     /gene="gatB"
                     /locus_tag="ACHA52_000265"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8939023.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13083..14138
                     /locus_tag="ACHA52_000266"
     CDS             13083..14138
                     /locus_tag="ACHA52_000266"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8939024.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14249..15625
                     /gene="rlmD"
                     /locus_tag="ACHA52_000267"
     CDS             14249..15625
                     /gene="rlmD"
                     /locus_tag="ACHA52_000267"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8939025.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15834..16670
                     /locus_tag="ACHA52_000268"
     CDS             15834..16670
                     /locus_tag="ACHA52_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8939026.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            16819..17040
                     /locus_tag="ACHA52_000269"
     CDS             16819..17040
                     /locus_tag="ACHA52_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939027.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            17041..19758
                     /locus_tag="ACHA52_000270"
     CDS             17041..19758
                     /locus_tag="ACHA52_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM8939028.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            19751..21706
                     /locus_tag="ACHA52_000271"
     CDS             19751..21706
                     /locus_tag="ACHA52_000271"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8939029.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            21696..22832
                     /locus_tag="ACHA52_000272"
     CDS             21696..22832
                     /locus_tag="ACHA52_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8939030.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(22883..23086)
                     /locus_tag="ACHA52_000273"
     CDS             complement(22883..23086)
                     /locus_tag="ACHA52_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939031.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(23102..24934)
                     /locus_tag="ACHA52_000274"
     CDS             complement(23102..24934)
                     /locus_tag="ACHA52_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8939032.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(24937..25383)
                     /locus_tag="ACHA52_000275"
     CDS             complement(24937..25383)
                     /locus_tag="ACHA52_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM8939033.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(25376..26326)
                     /locus_tag="ACHA52_000276"
     CDS             complement(25376..26326)
                     /locus_tag="ACHA52_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM8939034.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            26721..27338
                     /locus_tag="ACHA52_000277"
     CDS             26721..27338
                     /locus_tag="ACHA52_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939035.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            27435..27641
                     /locus_tag="ACHA52_000278"
     CDS             27435..27641
                     /locus_tag="ACHA52_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939036.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            27646..27960
                     /locus_tag="ACHA52_000279"
     CDS             27646..27960
                     /locus_tag="ACHA52_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM8939037.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            27961..29103
                     /locus_tag="ACHA52_000280"
     CDS             27961..29103
                     /locus_tag="ACHA52_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM8939038.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            29105..29647
                     /locus_tag="ACHA52_000281"
     CDS             29105..29647
                     /locus_tag="ACHA52_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM8939039.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            29708..31644
                     /locus_tag="ACHA52_000282"
                     /pseudo
     CDS             29708..31644
                     /locus_tag="ACHA52_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
     gene            31855..32625
                     /locus_tag="ACHA52_000283"
     CDS             31855..32625
                     /locus_tag="ACHA52_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8939040.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            32618..32962
                     /locus_tag="ACHA52_000284"
     CDS             32618..32962
                     /locus_tag="ACHA52_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM8939041.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            32955..35198
                     /locus_tag="ACHA52_000285"
     CDS             32955..35198
                     /locus_tag="ACHA52_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8939042.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            35336..35545
                     /locus_tag="ACHA52_000286"
     CDS             35336..35545
                     /locus_tag="ACHA52_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939043.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            35542..35826
                     /locus_tag="ACHA52_000287"
     CDS             35542..35826
                     /locus_tag="ACHA52_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8939044.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            35826..37178
                     /locus_tag="ACHA52_000288"
     CDS             35826..37178
                     /locus_tag="ACHA52_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM8939045.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            37175..37603
                     /locus_tag="ACHA52_000289"
     CDS             37175..37603
                     /locus_tag="ACHA52_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM8939046.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            37771..38136
                     /locus_tag="ACHA52_000290"
     CDS             37771..38136
                     /locus_tag="ACHA52_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8939047.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            38270..38842
                     /locus_tag="ACHA52_000291"
     CDS             38270..38842
                     /locus_tag="ACHA52_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8939048.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            38857..39639
                     /locus_tag="ACHA52_000292"
     CDS             38857..39639
                     /locus_tag="ACHA52_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM8939049.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            39639..40874
                     /locus_tag="ACHA52_000293"
     CDS             39639..40874
                     /locus_tag="ACHA52_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM8939050.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            40948..41325
                     /locus_tag="ACHA52_000294"
     CDS             40948..41325
                     /locus_tag="ACHA52_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM8939051.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            41327..41521
                     /locus_tag="ACHA52_000295"
     CDS             41327..41521
                     /locus_tag="ACHA52_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939052.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            41505..41681
                     /locus_tag="ACHA52_000296"
     CDS             41505..41681
                     /locus_tag="ACHA52_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM8939053.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            41678..41821
                     /locus_tag="ACHA52_000297"
     CDS             41678..41821
                     /locus_tag="ACHA52_000297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939054.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            41904..43469
                     /locus_tag="ACHA52_000298"
     CDS             41904..43469
                     /locus_tag="ACHA52_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8939055.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            43525..44259
                     /locus_tag="ACHA52_000299"
     CDS             43525..44259
                     /locus_tag="ACHA52_000299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939056.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            44325..45653
                     /locus_tag="ACHA52_000300"
     CDS             44325..45653
                     /locus_tag="ACHA52_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8939057.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            45553..46308
                     /locus_tag="ACHA52_000301"
     CDS             45553..46308
                     /locus_tag="ACHA52_000301"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8939058.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            46312..47523
                     /locus_tag="ACHA52_000302"
     CDS             46312..47523
                     /locus_tag="ACHA52_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8939059.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            47534..47845
                     /locus_tag="ACHA52_000303"
     CDS             47534..47845
                     /locus_tag="ACHA52_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8939060.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            47842..48180
                     /locus_tag="ACHA52_000304"
     CDS             47842..48180
                     /locus_tag="ACHA52_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8939061.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            48173..48604
                     /locus_tag="ACHA52_000305"
     CDS             48173..48604
                     /locus_tag="ACHA52_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8939062.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            48601..48942
                     /locus_tag="ACHA52_000306"
     CDS             48601..48942
                     /locus_tag="ACHA52_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939063.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            48945..49535
                     /locus_tag="ACHA52_000307"
     CDS             48945..49535
                     /locus_tag="ACHA52_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8939064.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            49545..49925
                     /locus_tag="ACHA52_000308"
     CDS             49545..49925
                     /locus_tag="ACHA52_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939065.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            50123..52693
                     /locus_tag="ACHA52_000309"
     CDS             50123..52693
                     /locus_tag="ACHA52_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8939066.1"
                     /translation="MIYLADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSE
                     FDKNDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLN
                     NAKAQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKF
                     GGVLKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNA
                     DNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDI
                     ESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEA
                     IHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQF
                     QNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTL
                     IPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNG
                     LIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQ
                     LLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIIT
                     TIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLA
                     NAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPST
                     LFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSM
                     LDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY
                     "
     gene            52764..53165
                     /locus_tag="ACHA52_000310"
     CDS             52764..53165
                     /locus_tag="ACHA52_000310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939067.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            53242..53934
                     /locus_tag="ACHA52_000311"
     CDS             53242..53934
                     /locus_tag="ACHA52_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8939068.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            53931..57821
                     /locus_tag="ACHA52_000312"
     CDS             53931..57821
                     /locus_tag="ACHA52_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8939069.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            57831..57998
                     /locus_tag="ACHA52_000313"
     CDS             57831..57998
                     /locus_tag="ACHA52_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939070.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            57985..58392
                     /locus_tag="ACHA52_000314"
     CDS             57985..58392
                     /locus_tag="ACHA52_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM8939071.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            58393..58812
                     /locus_tag="ACHA52_000315"
     CDS             58393..58812
                     /locus_tag="ACHA52_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8939072.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            58802..59710
                     /locus_tag="ACHA52_000316"
     CDS             58802..59710
                     /locus_tag="ACHA52_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8939073.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            59826..60863
                     /locus_tag="ACHA52_000317"
     CDS             59826..60863
                     /locus_tag="ACHA52_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8939074.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(60924..61229)
                     /locus_tag="ACHA52_000318"
     CDS             complement(60924..61229)
                     /locus_tag="ACHA52_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939075.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            61446..61697
                     /locus_tag="ACHA52_000319"
     CDS             61446..61697
                     /locus_tag="ACHA52_000319"
                     /inference="COORDINATES: protein motif:HMM:NF042617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8939076.1"
                     /translation="MKGKGKFVIGFTFMDFLRLYLEEAKAMTNKKTEINYSDEAAYYI
                     SQQILLNLLVTGKINLEEYVKIDNKNRETFNPFLSRIMV"
     gene            61758..63311
                     /locus_tag="ACHA52_000320"
     CDS             61758..63311
                     /locus_tag="ACHA52_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8939077.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            63359..63724
                     /locus_tag="ACHA52_000321"
     CDS             63359..63724
                     /locus_tag="ACHA52_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8939078.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            63726..65276
                     /locus_tag="ACHA52_000322"
     CDS             63726..65276
                     /locus_tag="ACHA52_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8939079.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            65399..69922
                     /locus_tag="ACHA52_000323"
     CDS             65399..69922
                     /locus_tag="ACHA52_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8939080.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTN
                     INYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYF
                     NSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIV
                     EQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSV
                     LAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLE
                     TFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQ
                     SIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIE
                     LLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIF
                     EIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAE
                     HYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPEL
                     SVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDI
                     LIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRN
                     SANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPEN
                     GNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQAS
                     SYLFKFKQKRSDG"
     gene            70057..70185
                     /locus_tag="ACHA52_000324"
     CDS             70057..70185
                     /locus_tag="ACHA52_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8939081.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT        22750 a        12520 c        15770 g        19203 t
ORIGIN      
        1 aaaaatatgc tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac
       61 aaaaaatcct agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc
      121 tttttaatgc ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt
      181 ttctagaaag agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata
      241 tgagtgaaaa atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg
      301 aaaccattaa tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga
      361 ttccttttct gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa
      421 atttagttga tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta
      481 atgctgtttt aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat
      541 ttatgggaat gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg
      601 cagctgttaa acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata
      661 ccaaattcaa ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa
      721 ccgagcgttg tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg
      781 caatacaatg atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga
      841 tgggttaaaa ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa
      901 agggatcaat gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt
      961 tttaggaggg ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc
     1021 tttgttcaca accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga
     1081 aactgaaatt aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca
     1141 agcattagga acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag
     1201 cctgcgaaaa ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt
     1261 cgtgattgat acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat
     1321 caaaccaaac aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga
     1381 aatcattccg tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat
     1441 ggctggagac ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt
     1501 tcgcccattg aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc
     1561 ttacagccgt acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc
     1621 aacagctttt tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt
     1681 tgaaatcaca aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg
     1741 gtcaaagaag cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa
     1801 atggtccaaa aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga
     1861 attctagccc gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac
     1921 gcgaaaaaca gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta
     1981 gattatgaag ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa
     2041 ggcgcaaacg acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca
     2101 gatttcgtag gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa
     2161 acagctgaag agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa
     2221 gcttctgctc atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc
     2281 gctcacactt acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag
     2341 atcaaagtcg aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc
     2401 gcccgtgcag aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac
     2461 ggaaaagaac tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc
     2521 aatctagccc ttagtggtac tgcaccagta ttccgtggtg atggaaaaga aagcagttca
     2581 gaagaaggaa atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc
     2641 gtatcacaca tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg
     2701 atcgaccaat ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa
     2761 gctgctagct ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta
     2821 gcaggattta ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct
     2881 ggtgcattag ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta
     2941 gctggttatg tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat
     3001 ggaattaaaa cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg
     3061 ttgctggtca atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt
     3121 atgagtggtt cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat
     3181 ttaggcggtc caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact
     3241 gttgcaactg gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca
     3301 ttggcaattt tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat
     3361 gctggtttga cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt
     3421 gcagcagctg acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc
     3481 ggattagtcg gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta
     3541 ttcttactga gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct
     3601 ggtgtgattt atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa
     3661 agaagaggct ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag
     3721 tgtagatgaa tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa
     3781 tttgtgatag aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca
     3841 gtcaagataa ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc
     3901 tgatttcctc tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc
     3961 attaatccgt ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct
     4021 tttagtgagg cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga
     4081 ttaccgccga gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc
     4141 agatgattaa gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt
     4201 ccaatcgctg tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga
     4261 tccatcccga aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc
     4321 aatatcggaa ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg
     4381 aatcgaagaa caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct
     4441 aaaacgattt gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc
     4501 agcaagaaag gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt
     4561 ggcaaagcca gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag
     4621 atcactaagg aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca
     4681 ggtctcaagc cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat
     4741 gtcagccaaa caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct
     4801 gcgataggag cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc
     4861 ttttcttctg aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca
     4921 agctgtatag aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg
     4981 ccaatgaact ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt
     5041 ccctctactt gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc
     5101 acgaaaaatg ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc
     5161 cgactcattt catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac
     5221 aggctaacga cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc
     5281 tgagtcttat ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt
     5341 ttaatttagc gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat
     5401 tttgaactag ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa
     5461 gaaaggatgt tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag
     5521 aaagaagctg ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga
     5581 aagacgagag tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct
     5641 tggaacatcc tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc
     5701 aatgcgattc tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt
     5761 gtgcgtattt taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc
     5821 gattcttctg aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg
     5881 aaaaaatatg atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag
     5941 acccctcagg attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt
     6001 tatgctgctt accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc
     6061 atgaatacga ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa
     6121 ttccattata ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc
     6181 aatttattgg caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt
     6241 tacggctggc gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat
     6301 gctgcagtaa ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca
     6361 aaccaagtca ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa
     6421 gaaggaaaca agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc
     6481 gtagatcgga tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc
     6541 ttgtaccgga cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt
     6601 ccttataaaa tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta
     6661 gcgtatttga atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat
     6721 tcgcccaaac gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt
     6781 catgaatggc cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa
     6841 gcaggacagc aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca
     6901 tacttgacag tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac
     6961 ttaaaaatcc aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta
     7021 acggtcacac aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca
     7081 ccagaagaaa aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat
     7141 ttagaagaag atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa
     7201 ttcccagttg tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg
     7261 atggaagaaa gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca
     7321 gaagaagcgt tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat
     7381 aaccgacctt ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg
     7441 cgtcctacgc ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag
     7501 ccagagacag cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa
     7561 cctggagata aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg
     7621 acgtcgaagg atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg
     7681 gctgcctttg cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat
     7741 gacgattgac gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc
     7801 tcgcgaatac tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata
     7861 cgcagaatta gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac
     7921 tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct
     7981 ttacagtttg aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca
     8041 aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt
     8101 ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt
     8161 aggagaaaac atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga
     8221 accaatgaac atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt
     8281 gaaccaagac agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc
     8341 tggttctttg cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt
     8401 atacacagta gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga
     8461 gctagaaaaa attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga
     8521 agtgtggtca tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga
     8581 tggaatcgtc atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa
     8641 agcaccgaga tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga
     8701 aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc
     8761 agtccgagta gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa
     8821 gttgaaggat atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc
     8881 agaagtttcg caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc
     8941 tacacattgt ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg
     9001 gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag
     9061 aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa
     9121 actagtagca gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga
     9181 caaaataaaa gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa
     9241 ttcggtcgag cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa
     9301 aattttagca gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat
     9361 tattgcgctt gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa
     9421 cgaagaagtc catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa
     9481 agggcttcga aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt
     9541 ccttactggt aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct
     9601 tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga
     9661 tgctggcagc aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca
     9721 aatggcagat gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag
     9781 acaaatttca ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt
     9841 tttaactcgt ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt
     9901 tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt
     9961 aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt
    10021 tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg
    10081 gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc
    10141 cgcaatcatc gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta
    10201 gaagaactgc atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca
    10261 acatttgaac gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa
    10321 gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc
    10381 cttgctggta ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc
    10441 gctgcatcta agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa
    10501 atctatcaag cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt
    10561 ggaagtacag aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta
    10621 cctggtggct cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct
    10681 ttaggtagtg atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg
    10741 atgaaaccta cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt
    10801 gaccaaatcg gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc
    10861 agcggttatg acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag
    10921 ggactgacag gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa
    10981 ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta
    11041 ggtgctgttg tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat
    11101 atcattgcct catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt
    11161 tatcgctcag aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga
    11221 ttcggtgaag aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat
    11281 tatgacgctt acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa
    11341 aaagtctttg cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga
    11401 atcggtgaga acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct
    11461 gtcaacttgg ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca
    11521 gttggattgc agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat
    11581 gcgtttgaac aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac
    11641 gaatgaattt tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa
    11701 aaattttctc tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga
    11761 tcgactgggg ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga
    11821 tgaaagcagc tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa
    11881 attactttta cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg
    11941 gtcatgacgg atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac
    12001 gtgtccattt agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg
    12061 ttgatttgaa ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt
    12121 caccagaaga agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag
    12181 tcagcgatgt gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc
    12241 cttatggaca agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt
    12301 tcgtgaaaaa aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag
    12361 gagaaatcca acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc
    12421 gtgtcaaaga agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg
    12481 tgatcgatga cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc
    12541 gtcgcgaacg ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac
    12601 ttacaaaaga aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc
    12661 aagcatctaa ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat
    12721 tagctgagac aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg
    12781 gaacgatcag ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag
    12841 aagcccaaga agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac
    12901 tgccaatcat taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg
    12961 gaaaagatcg tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag
    13021 caaatcctgg tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga
    13081 aaatgaaaaa agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa
    13141 atttggcgga tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa
    13201 ctacaccaga accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg
    13261 atctagtagt ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc
    13321 cgcttgaaca ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc
    13381 gagcattaaa aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc
    13441 aaaccgtaaa aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag
    13501 gtggctattt gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt
    13561 atcttgctta tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc
    13621 gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga
    13681 cgaactctgt tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt
    13741 tttctttaat cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca
    13801 tgctgaatgg cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt
    13861 tgttcgttga aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat
    13921 acggcgggga agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca
    13981 ataccgatca gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg
    14041 tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg
    14101 gaaaaatagc agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta
    14161 gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga
    14221 ttgaaaaata ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga
    14281 acgatataca gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga
    14341 tggttatcca ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt
    14401 aaaagcaggc aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga
    14461 tcgtcagcct gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt
    14521 gaagtatgaa caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat
    14581 tgctaagatg ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta
    14641 ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta
    14701 caaaaagaat agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat
    14761 cgatcaagca atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa
    14821 tgaagaaaaa aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca
    14881 tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc
    14941 tgaagctatt catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga
    15001 aaaaacaaat gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga
    15061 agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac
    15121 tcaacaagca gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga
    15181 cattgtgatc gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt
    15241 ggataaggtg tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc
    15301 tttaaataat aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa
    15361 aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg
    15421 attagatcat cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat
    15481 ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc
    15541 cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc catcacatcg agtgtgtagg
    15601 actgcttaca cgtgtggaac gttgatatga caaggattag agcgattcgg gatggtttcc
    15661 ggattgctct tttttcgtgt tgaaatgacg atttttgggt gagcgtagag ttgatggaaa
    15721 tgctatggag ctttgagttg ataggagtta caccgtagac ttgataggtt agatgatttt
    15781 gataacggtt acgtgatata atatattatc gagttttcaa gggagacaaa cttatgaagc
    15841 aaatttttat attttcagat acacagaaag ataatatttc ttattttgaa cgcgagccag
    15901 caatcttggt ttcaatgagt cgtagtgatt tgccgtattt gaaggaacag gatttttcaa
    15961 attatccagc tgtttatgtt ttgattggtg gtaataagcg ttacgtgggt caggcagcag
    16021 ggcaatcaat ttcacttcgt ttatctcaac actttctcaa ggaatataaa gcttgggttg
    16081 aatcagtttt attctttaca agagcagatg ggaaaatgtc aaaagcagat acagattatc
    16141 ttgaaaaacg acttatccaa gatttccaag aaaaatctga ctatgaaatg atgaattcaa
    16201 ctgctggtaa tacttcgtat attgataaac ttcaaaaagc aaaatcggat cagctatatg
    16261 acacagtgtt tgaaattata gatgaaattg caaatatcga tttgtttggt acttccgaag
    16321 acagtgaaac ccaaatttat gaatttagtt catctggaca atatgaaatt gagtatgatg
    16381 ggaaaaagat atcttcgaaa tctgctagag gtttattggt tgattttgtt tctcagctat
    16441 ttgcggatgg aaactacaac aatcaactgc aagaattaat tgtcgatgag acgccgtcgt
    16501 tcgctataat tttaggacgt aaaccatcca tgtataacgg aagaccaaat tcagccaggg
    16561 tagctgatgg agtatgggtt tatgcaaatt tcaacaaaaa gaatgtcaga atcaaaattg
    16621 ataaactagc tcgtcaattg gggattccgg ttaaaataaa gtggaattaa tatttgttat
    16681 ctcgccttta gtaaatctat gctgaaggct tttattttag aaaaatagtt tgcgttcgcg
    16741 aaaatatttc atgtttcgta taataatgat agctcataga tgatataatt gaagatacca
    16801 gtagaataga ggtgtagtat gtcaatttct tataataaat tgtggaagtt attgattgat
    16861 ttgaatatga acaaaactca acttcgtgaa atatctggcg tcagtcccaa tgtaatagca
    16921 aaactcggta aaaatgagtt ggttgcaatg gagtcattat tgaaaattgc tgctgcctta
    16981 aatgttgatg ttagtgaaat aattagttca gatgttatag atgcggagga tgttaaataa
    17041 atggcaaaaa ataaagtttt aaacatcact gaaattgaag atggtgtaaa aaaaatactt
    17101 tcaaataggg gcgatgattt catcactcaa tttttgtcct tgtacgatat ccccaaaacg
    17161 tcgattacac gtgcaaaatc aaaattcgat aatggtgaac catttgtgat taagaacaaa
    17221 cttcattatg aagaaattga aggcaacgtt gtcattgcga ttgatgcgat tgctcaatca
    17281 atcaaagagc aaaaatctaa accacgatat atcttggtaa acgattattt agatattgct
    17341 gcaattgata cgaaaactca tgaaactttg aatattccaa ttgaagagct accggctaat
    17401 gctgactttt tcttagcgtg gaatggcatt gagaaggctg attatcaagc tgaaaatcca
    17461 gctgaccgaa aagctgctga acgttttgca aaattgtatg atgtggtggc taaagataat
    17521 cctgatgcca acgagcatgc atttaacttg ttcttgattc gagtgctgtt cttgttgttt
    17581 gcagaagata caggaattat gaataagggt tcctttacaa atgttctaaa aacaagaacg
    17641 gctgaagatg gttcgaattt taacgaagta atcaaagaac tctttgcaat cttggatatt
    17701 aatgagttag ctcgcaatgg caaggatgag tggctacttg aatttccata tgttaatgga
    17761 aaactgtttg gtgagccaca tgtggatttg accttttcaa aagtttcaag agagctgctt
    17821 attgaagcag gggaattatt gaattggaat gaaatcaatc ctgatatcct tggcgcaatg
    17881 attcaatcag ttgcttcagc agaagaccgt cacgttgccg gtatgcatta tacaagcgtg
    17941 ccgaatatca tgaaagtcat caagccgtta ttcttagatg atttgacaga gtcatttgaa
    18001 actttaaagc ttcgcagtga agaaaacgag ctgaaggaca ttactgaaaa aacaagaaac
    18061 gataataaga gaacaatcat caatgatcta agtgctttgt tgacgcgaat ttctaatatt
    18121 aagtttttgg atcccgccag tggttctggt aattttctga tcattgctta taaggaaatc
    18181 cgtagacttg aaattaagat tttgcaattg ttgggcgact tacaaaataa tgattccatg
    18241 ccactatcag tgattcatct tggtaatttt aatgggattg aattggatga ttttgcgcat
    18301 gaagtagcaa gactttcatt atggattgct gaacatcaaa tgaataaaga gcttgaagaa
    18361 gcaataccag gtaccattgc tgagttgtta ccgttaaaag atgctgggaa tatagtgtgt
    18421 gccaattctc ttcgaattga ttggaatgag gtgttgcgac tcaaagaaaa tcatgaaata
    18481 tatataatgg gaaatccacc gtatctgggc aaaagtcttc aaaatgctga acaacgtgaa
    18541 gatatggaaa aacttgttgg cgatattcga aaatataaat tgcttgatta tatttttggg
    18601 tggttttgga aagctaaaca atatattgca acatctcatg gctcgttcgc atttgttact
    18661 actaattcaa tttgtcaagg tgaacaagtt agtatccttt ggccgtacct tttaaaagat
    18721 gaggtaagaa tttcatttgc atatcagagc tttaagtgga acaacaatgc aaagaataat
    18781 gccggtgtga ctgttgtcat tattggtatg acaaataatg tgaatattca tcctaagtta
    18841 tatttagaga atgggtcagt aatgagtgtt tcaaatatta atgcatattt agctaatggt
    18901 gaaaatgtat ttgtcaacga atcaagaaat agtatttcaa atttaccctc tttgaaattt
    18961 ggcagtatgg caaatgatgg tggtaatcta gttttttcag tggcagaata ctggaatttg
    19021 attcaaaaat atccagatat taaacattta tttaagaaat atttaggatc aacaggatta
    19081 ataaatggag acttaagata tactttatgg ttgaccgagg aagatgcaac tagatacaaa
    19141 caaaattctc ttattaaaga aacaataaaa aatgtaaaaa gttataggga aaaaagcaag
    19201 agaaaagaaa ctcgagcact agctgagaag ccatggtcat ttggtgaagt tcgttatgat
    19261 gaaaccagtg agaatagtat tctaattcct cgtgtgtcat cagaaaaaag agattatgtt
    19321 cctatgggta tagttgggag cgatactata atttcagatt cggctatggc aatctataac
    19381 gctccaatgt ggctgcttgg tctgttagaa tcccgaatgc atatggtgtg gctgcgctct
    19441 gtcggtggga aattagaaac tcggtaccgt tattcagccg ggttagtcta taatactttt
    19501 ccgattcagg atttatccgc ccagcgtaaa aatgaaatga ctcgcgtgat gacagaaatt
    19561 cttgatttac gagaatacga aggcggcacg ttagccgatt tgtataataa agacagtatg
    19621 ccagaatcat tacgtaagaa acaccaggaa ctggatggga ttgttgatag agcttatcag
    19681 caacgcccgt ttgaatctga cgaagatcga cttggaacat tactaaaact ctatcaggag
    19741 atgacaatca atgactagta atttatttga aattaatttt gataaaaatc aatcaaaaac
    19801 aacaaatgac ctcggtatgc gtgaaatgca agaacgtgtt tatgaaaagc gtgcgtcaca
    19861 gtatttgttg gtaaaatcac caccagcttc aggaaaatca cgtgcattga tgtttgttgg
    19921 cttggataaa ctccataatc aaggaattaa aaaagtggtt attgcagttc ctgaacgctc
    19981 aattggtaaa tcatttaaag atactgaatt atcgaagttt ggtttttatt gggattggga
    20041 agtaaagcca agtgacaact taactattgg gggcggtgaa aaatcaaagg ttcaacactt
    20101 tgttaaattc atgaactcga ctgacccaag tgataatatc ctgattgcca cacatgctac
    20161 gttgcgattt gcttttgaag agttggacga ttcattattt gacaatacgc tattagcgat
    20221 tgatgagttc catcatgtga gccgtgatga taaatctgtg ttaggtaatg ccttacgtag
    20281 catcatgtca aactctaatg cgcatatctt agcaatgact ggttcatatt tccgtggcga
    20341 ctctgttcag attctggaac cagccgacga agataaattt gataaggtca cttacactta
    20401 ttatgagcaa ctggaaggat ataaatatct gaaatccttt gcaatggggt atagtttcta
    20461 tcgtggaaaa tatacagatg ctttggataa agtaattgat gtcaacaaaa aatcaattat
    20521 ccatattcct aatgttaatt ctggtgagtc tactaaagat aaatacgcgg aagttgatga
    20581 aatacttgat ttattgacgg acggtggtaa tatcacacag aacgaggatg gtatttatga
    20641 aatcgaacgt ccagatggta aaaaactatt agtggccgac cttgtgaatg aagaaggacg
    20701 tgaaaaagtc tcagcatatc tagcaagtat tacggatgac attaaagatc ttgataagct
    20761 tgatatcatc attgcacttg gaatggcaaa agaaggcttc gattggccat ttgcggaata
    20821 tgcactgaca atcggttatc gtaactcatt aactgaagtg attcaaatca ttggtcgcgt
    20881 aactcgtgac agccccaata agactcatgc acagtttaca aatcttattg cgcagccaga
    20941 tgcacaggat gacgaagtat tattcgcggt taacagtatt atgaaggcaa tttctgcatc
    21001 tctacttatg gaacaagtgt tggcgccagt ttacaagttt aaaccaaaag atggtaagcc
    21061 agacaaacca ggtgatcttt caatcagagg actgaaacct gctgatacga cggaacgtac
    21121 aaagaaaatc atcgaaaatg atatggaaga tttgaaatct agcattttac agagccaaga
    21181 tattcaaaat gcgattgttt caggtgctga tgcgaagatg attaataaga cgttgattcc
    21241 acgtgtcatc attgatcgtt atcctgattt gacgaacgaa gagttagaaa ccgttcgtca
    21301 acatacagtt gccgatataa atattgcggc tggacggaca acaacttcag aagatggtag
    21361 ccgcgaaatt attaagatgg ccgatcgttt cattaacatt gatgaattga atattgattt
    21421 aatcgatcaa gtgaatccgt tccaacgtgc gtatgaagtg attagtcgtg atattgatgc
    21481 accgactctt cggtttattc aggattattt gtcatcaaat aagtatgatt ttactgacga
    21541 acaattggtt gcggcctacc aagctgctaa gaagttccgt gcagaaaatg gtcgaaatcc
    21601 tgatcgaaat agtaaagatg atggtgaaaa atacttggcc tttgctttgc tacgacttgc
    21661 tgagataaaa cgtgaaagag aggcagcgaa agaagatggc gaatagttta gatagcatct
    21721 ttgaagatga cgcttttgat gatttaacca agcaagttga aaaaaagaaa gttgtaaaac
    21781 aagacccaga agttgctaaa tttcaggaga ttatagactt tgtgaatgaa aacggacgag
    21841 agccagaaaa aactcctgaa tggtctagtg aacgcgcatt gtgggctcgt cttagcggat
    21901 ttcgaggaaa acccgaaagg tctgagaagg ttaagaagta tgacactctt ggtttattgg
    21961 agaaagaatt cgaaccggat ggagagtttc tcgctgattc agttgaaatc gatgacgagt
    22021 tgaatttaga tgatatctta ggctcagatg atatgattga tgatttttca gacttgttgg
    22081 atgtttcccg ctatcgtaga actgtgaacg ctgcagataa aattggccgc agaaaacatg
    22141 caagtcattt tgaaaagtat aagagcttgt tcgatcaagt gcatgcagat atcgcatctg
    22201 gcagacgaaa aattatcccg tttgagcagt atgatattca tgaaaataga ttctacatcc
    22261 aaaacggcgt aatgatgtat atcgtttcca tttcgaagga tacctttatc gctgataatg
    22321 ggaaaacaaa tgccagaatg catgttgttt atgagaatgg gacagagaat aagaatctgt
    22381 tgttacaatc tttagcttcg tctctatact cgactgaacg tcatggaagg atggtgacag
    22441 agccggtaga tgaggaaaca ctggctgaaa gctttggaga agagtttacg acaggttata
    22501 tctacgtgct gaagtcatta agcacgaatc cggatattag caagtggaat aacctgtata
    22561 agattggttt cactcgaaat gatgttgaaa gtagaattgc caatgcggaa aacgacgtga
    22621 cctacttaaa tgcaccagtg aaggtcatgc tttctgctga agtacaaaat gtcaacgctc
    22681 agctacttga gcgcactctc catcatgtat tccaggacaa acaagttgtc tttcaggatg
    22741 gaaacttcaa aaaagctact gaatggtata tggttccatt gaatgatatt gaatcgaaaa
    22801 tcaatgaagt aatcgctgga ttacaaaaat aaatatgaaa atgcactgta ctcatgaatc
    22861 gaatacagtg catttttgat atttaatcgc gaacgatttc aacaatatcc gataaatcac
    22921 aatcaagtgc ttcacagatg cgaagtaaga tatctgttgt tacattttcg cctttattta
    22981 gttttgagag tgttgctgaa ctgatatcag tcatttttct gagttgtgct ttattcagtc
    23041 ctctgtcgat taggagtttc caaagcggat tgtagctaaa cttcatgtaa tcattcctgt
    23101 tctagttata aatatcgtat agatatccat agcgtccatt gtttttatct tcagtaaaga
    23161 caatctgatt tacttgttta tcatcatagt ttagtgctgg caagttcgtt gtattctcaa
    23221 cgatgataag ttgtccttcg ttttgatgat ccataaagta tttgaaaagt ccttctttca
    23281 tattgtcatc gccatcttgt tttttgcgtt catctaatcc aagaagaggg gaatcgataa
    23341 tgaacacaga aggtgtgtac ttagcgtgat tatacaagag atgtcgatat gccattacca
    23401 ctactgaatt gataaacgct gtatatccat gtccgttttc ggtccatttt agatttccat
    23461 tgattttgat gtcgaaaata tccttggcga aaaatgcgtt acgaaattca ttaggtttat
    23521 aatgcatatt acgtaacatg gagtccagta actcagacat ttctttgtag aattctgcat
    23581 caaatttctc tttaggttta taaaggatac gttcgtcttc tggctcattc tcgatttttt
    23641 caagatcact tttaagctta ttttgcattt tttccatcga gattagctgt tgtttaatat
    23701 ccctgtaagt ttcgatttta ttgattcgat cctttaacgt ctcagctttc ggttttagtt
    23761 catcgttgat tgctgcatca agcgatttct ttcttgtttt aagctcatca agctgttcca
    23821 ttaactcttg cagttcaact tcggtatcat gagattcttc cgtcaaatct gataattgag
    23881 agagcaatga atgtaattct gacctagcag tatcgatgta ggttgtttgg acaggtttac
    23941 ttagttcgcc gttacagaat ggacagctat gtgaagtatc atgttcatta tgatcattta
    24001 ataatcgctc accatctacg attagtgtta gtcgttcaat atccgattga tattgataat
    24061 gtaactgttt gtacctattc aaaagaagtt gagtttcatg aagatactgt tccttttgat
    24121 aaatctcttt tatcaagatt ttgctgctgg ctaacgcatc attgatttgc ttttcaattg
    24181 tttcaagttc agcgaacatt ccatcgacaa tttgttgaaa atcatcagag ggagcctcag
    24241 gtaattctga tttagccact tcgtagtttt catataattc agaaaggctt gaattgatgt
    24301 agttaatcac cgagttcctt ctaatttttt tatgttcttt tgtttcgttt tcttccgagt
    24361 tcgcaaaatc tgaaccgtat agcagaagta acaacgatga taaaaaagct gtgcgttggg
    24421 ttgtttcccc aggaaaaaga acagaggcgc tggtattgac tctatcttgt ggaataagtg
    24481 tgctaggcgc taatgtccgc caagttaatt tttgacgttt gaaatcctca ttcttgataa
    24541 tctgataatc atcatcgatg cctaaaagtt gtaaccaaaa atggctaatt ggatttttcc
    24601 cagatcgtgc attatatttt tcataatcta catattcacc tgaactcgtt attttggata
    24661 ccaaaatgga gttgctatca aattctctag ttaatgaata tttgccatta gtcgattgaa
    24721 taatcagttc aactttactg tatccggttg catcggttga aaagggagca gcgtcttttt
    24781 tcgcgccata gagaaaatcg attgccgtaa caatgctagt ttttccggta tttgaagggc
    24841 cagaaattat gtttagtcca ttggtgaact tgacagttgc atcttcacga tctttgcctt
    24901 ttatggtaac acgttcaata taataatttt gcattatcat tgctctcctt taaattcctt
    24961 tgcgcgttgg tttatttgtc gcagaagctc aacgtcactt ttatcttgaa tgtatttaac
    25021 agattcgtca gctatttgta agtatttttc tgcatatacc gatacgaatt gtgatatcaa
    25081 ctttaggcca tcttctgata aagcatactt aaaaccttct ttgctttgcg atggttggat
    25141 aacatgttgc agtacaagat ttttgactgc atctttaact aattcacgtc gcgcaataaa
    25201 ttcactgaat ctatagctgt tatcaccatt taagttgtaa tcggaaattt caaagtcgcg
    25261 accatacgtg gtgatgaagt ctgtgattaa aattctatca gcgctgacag gctctttaaa
    25321 ttcagagagc agtaacatta ctcgaaccga aatttcaaat ggagtattaa acaaactatt
    25381 catcttcagt cacccatttg atctttccgt cattaaccag catgtggcaa atgccttttt
    25441 tagcagaatt ttgcagcaat cccggtataa acgataagat tgatccgttt aatgttataa
    25501 cagacgactg ctgtagaacg gctttgagtc tctcaacgcc atcatcgtag tcttcttcat
    25561 agacttcctc gatgccgtcg tacatatctt cttttaaagc ttcaaagtgc tcagttcctt
    25621 cctcttgtgt aaagttatca cggaccccac gacgtatgct ttcagcttta tagtaatttc
    25681 ttcgctgtct cttgaaatcc tttagccgcg aatttggcag gtcgtttgta tgctcgtata
    25741 cttcgtatcc atcggcatct gcatatgctg aaaaaagagc tatgacatac gtcatctcct
    25801 cagctctaat atcaagggga actggcaaat cgtcaaagaa ttttttcact tcatcattat
    25861 aagagaaatc accattagtg ttgattctta aacttgcgag ctctaattcg ctaagcttcg
    25921 gcttggcctt ttttgttttt acttttgttt gagaagcaat tgaggtgatt tcatcaagta
    25981 aaagatgggc cactatttca gaaacgtctg tatctttggg gattaccaca cctttatcaa
    26041 tcaattgttg cttaaggacg gttggagcat catctataag acgttcataa ataaaagtcg
    26101 tgcatttgtc gatgttgaat tttccagcaa cccgaactgc atactcttgt gttaatggtc
    26161 gagtaccatt gtaaaatctt cgtaggttat catcagaaaa accgaaaatg gtggcctcgt
    26221 catcatttga aacactatcc agtaaagcaa ttaaaaaatc ctgtggcttc acgtactcgc
    26281 ttcccataaa cggataaagc gtttgagtaa tttcacttat ttccaaaata caacctcctt
    26341 tgttacgtcc gccgttgacc gccgtttgcc gccgatgact gacgaaaaca agggaggcct
    26401 ttttctataa ttaaaacata gtagtttaac tatttaattt tatcatttat ggttcataaa
    26461 cactacatta tgtttcaaga aagtgaacga atttatctta aaagcgaata gtcctactaa
    26521 taaaatataa caatgcctga gttgcaatag ggcaaggtta caaatactga aaagaactgg
    26581 ttatccagaa aatttttcag atattgtatg ccttacttta ttgcgctctt ttttcgtgat
    26641 gagggacggt gtacgtattg gcacggtccc tgtttgtttt ccttgctcaa aagctctttc
    26701 aggcaagaaa gagggatcta atgagtagat ttatgaaaac acaccaaaac gaccgcagca
    26761 cttacaagta cgtgtttgag cgtactgatg ccaatggacg tatccaaaaa gaagtggttg
    26821 agattcgccc aggcgaagac ggcgtcactg agcttaacat tcaggagctg catcgcgctg
    26881 acgaccgtgc gatttacaac ttttataaaa acgcacgtcc tgaacgcaca gaagaagaaa
    26941 aagaaaagat caaggcttgg aaagagaagt tccgtgaaga ctttaataat gagcacggct
    27001 attatccaat ggacgaatac gtggatggtg aagccaacga ttacttcaaa cgtaactaca
    27061 atttgtcgct caatgaattt gatgatgaat gcgatgaggg ggacaacccc atccaatcat
    27121 tggtttacga tgcttaccaa gataatgaag aagatcctcg tatcgatcgt ttgcgtgaag
    27181 tcgtgacgca attattaccg taccaccaag aaatctatca catgctcgtc actgaaaaca
    27241 tgtctcagac tgaagtggcg gacaagcttg acaagaaaaa acagtcaatc aatcgcacct
    27301 ttggaatcat caaagacaag atcaaagaat tattttagaa aaaagttacg ggggagttta
    27361 ctttccccgt tttccttcgc ctgtattgtg taagggagta gatcctaaac aaataaagat
    27421 gaaggaggac catcatgaac ctaaaacaca aaatccgagt aaacgtgact gatgacacgg
    27481 gtaacaaaga aactgtccta caaggtggtg tgcgtcattt gcctcgctgg tttgcacggt
    27541 ggctgttcgg taagggcaac atggacaaaa ttctcgtgct cacaccaggg gacacaatcg
    27601 aatcagtcga aatccgtgaa gttaacaaga agggagcgta atgacgtgag taaaacaaaa
    27661 ctatttcttc aggtcgttga ggacgtccaa gcgttagctg atagcttaca agagttggct
    27721 gtggcgatga aaaccaatga acctgaacca caagttgaaa caccagaacc gacaatggaa
    27781 acaaaatcag aagtcaaaca accgacgctt gaagaagtcc gtggtttgtt ggcacgtaaa
    27841 tctcaagaag gtaagtcaac tgaagtgaaa gcactgattg agaaatatgg tgccagtcgc
    27901 ttgagtgatg tgccgacaga aaactacgcg gctttggttg cagatgcgga gggattgtag
    27961 atgccaacac aacacgcagt cctatccgca tcttcaagta atcggtggat tcactgtccg
    28021 ccaagtgttc gcttgtcaga gcactttgaa aacaagtcga gcccgtatgc ccaacaaggc
    28081 actgatgctc ataccttatg tgagtacaaa ctgaataagt tcattggtaa tgacgtcaca
    28141 gacccaacat ctacgcttga attctacgac gaggagatgg agcagtgtgc tgaaagttat
    28201 gcgaccttcg tcatggaaga agtcgctaag gcgaaacaga caacagttga ctctattgta
    28261 atagtagaac agcggctcga cttctcaagg tacgtcccag acggctttgg gaccggcgac
    28321 tgtctcgtca ttgcggatgg aaccctatct gttattgaca tgaaatacgg tctaggaatt
    28381 ttggtagatg cgtatcagaa cccacagatg atgtgttatg ccttgggagc gttggagctt
    28441 ttcgatgtta tttatgacat tcaagaagtc aagatgacca ttttccagcc aaggcgtgag
    28501 aatgtgagta cgtacacact gctgaaagaa gaattacttc agtgggcaaa agatgtgttg
    28561 tcgcctgccg cagatttagc tttcaaaggt gagggtgagt accaatgcgg gaaatggtgt
    28621 ggattctgtc cggcgaagaa tagctgtcgt gcacgagcag accataactt gaaactggcg
    28681 caatatgagt tcaaaccacc agagcttctt agtgatgatg aaatcgaaga aatcatcggt
    28741 aaagtggatg acctggtttc ttggagtaac gatatcaagg actgtgcttt gaaacttgca
    28801 ctcggtggca aacaatggac tcatcacaaa ttagtcgagg gtcgctctac tcgtaagtat
    28861 tctaatgaca atgatgtcgc tgcggcggtc atcaaggctg gatacgaccc atatgataaa
    28921 aagttacttg gtgtcacagc aatgacgaag gcactcggta aagagaaatt caatgaaatc
    28981 ttgggcgaat acatcatcaa accaaaaggc aagctgacgc tagtcgatgg ttcagataaa
    29041 cgacaagcgg tcacagtaaa caacttaaac gaagaattta aaccattaac ggaggaacaa
    29101 taatatgtct aaaacaagca aagtaatcac tggtacaaac acacgtctct catacttcca
    29161 cggttgggaa ccagcatcca tcaacggggg cgcagagaaa tattcaacag cagtcttgat
    29221 tccaaaatca gataagagaa cgattgatac cattgaaaaa gcaatcgatg cagcaatcga
    29281 agagggactt gcgaaattcg gtgggaagaa accaaacaaa gcagaaatca agctgccatt
    29341 acgtgatggg gatatcgaac gcgacgatga agcataccaa ggccattact tcattaatgc
    29401 gaactcaacc actgcaccac aaatcgtgga tcagcacgtt caaccaatct tggatcgttc
    29461 acaagtgtat agtggttgct acgctcgtgt gtcgattaat ttctatgcct ttaacgcaaa
    29521 tggaaatcgc ggggtagcgg cagggcttgg gaatatccag tttgtacgtg acggcgaacc
    29581 actcggctca caagtatctg caagcgatga gtttgaaacc ttggcggatg atgatttctt
    29641 ggcttaatca ttaaaacaaa aacggccatg gcagaagtgc tgtcgcctgt ttgattggag
    29701 gaagaaaatg aaacatttgt cattggattt ggaaacgtat agtagtgtcg atttatcgaa
    29761 atgtggtgtc tacaaatatt cagagtcccc cgattttgaa atactgctat ttgcatacag
    29821 tgtagatggt ggtcccgtcg aagtggttga tctgaagatg gacgaaacta tcccggctga
    29881 aatattaact tcgctatcag atgaaacagt gattaaacac gccttcaatg cgagttttga
    29941 acgtgtgtgt ttgtcacgct ttctggggtt ggatggcttt ttgaatccac gagggtggca
    30001 atgcacaatg gtgtggggtg cgacattagg tttgccgttg tcacttgtag gagttggtgc
    30061 tgttcttggt ttggataaac aaaagctcac agaaggaaaa gacctcatcc gatacttttg
    30121 cgtgccatgc aatccgacca aagttaatgg tggacgcacg aggaacttac acaccatgcg
    30181 ccaactaaat ggtcagcatt taagacatac aatagtcgtg atgtggaagt tgaaatagct
    30241 atccaaggaa aactttccaa ttttccagtg cttgattctg tttggaaaga atactggctg
    30301 gatcaggaaa tcaacgaccg aggaattgag attgataagg actttgtgga agctgccgtt
    30361 cagttggatg aattatcaaa agctggattg aatcaacaat tgcaagaatt aactggacta
    30421 gaaaatccaa acagcgtgct tcaaatgcgg ggatggttac tagaccacgg acttgaaacc
    30481 gagtcactcg gtaaaaaggc agtggccgaa ctactcgaac aacctattga tgaaaattta
    30541 aagcaagtcc tggtcttacg tcagcaactc gccaagtcat ccgtgaaaaa gtatcaagcc
    30601 atgcaaaact ttgcgtgttc tgataatcgt tctcgtggtt tattccaatt ctatggagcc
    30661 aaccgtaccg gacgctttgc aggacgtggc atccagcttc aaaacttgcc tcagaaccat
    30721 atgtcagatt tatctgaagc gcgtagttta gtaaaacaaa gcaattttga aatgcttaac
    30781 attctctacg gcaatgtccc gaacgtactc tcagaactcc tccggacagc gtttgtacca
    30841 cgtgaaggga tgaaatttat cgtatctgac ttctcagcga ttgaagcacg tgtgattgct
    30901 tggctgtcgg gtgagaagtg gcgtcaagaa gtattcaaaa atggtggcga tatttactgt
    30961 gcgtctgcca gtcagatgtt cggtgtgcca gttgaaaagc atggcgtgaa tgggcatcta
    31021 cgtcaaaaag ggaaaattgc tgagcttgct ctagggtacg gaggatcggt gggtgcattg
    31081 aaagcaatgg atgctcttga aatgggacta actgaggatg aattacagcc gcttgttaat
    31141 tcttggcgag actcaaacat taacataacg gagctttggt gggctgttga taaggcagta
    31201 aagacttgtg tgaaagaacg acggagcaca gcgacgcatg gcatcaagtt tgaatacaag
    31261 tcgggttttc tgttcatcca actcttgtca ggacgtaaat tagtttatgt aaaaccaaag
    31321 attggtgaga acaaattcgg cggtgaagct gttacctatg aaggtgtcgg tgctaccaag
    31381 aaatgggaac gtcttgaaag ctatggtccc aagtttgtcg aaaacatcgt gcaggcaata
    31441 gctagagaca ttctctgttt cgcaatggag aatcttcgta agtatcgcat tgtcgggcac
    31501 gtccacgacg aagtgattat cgaagtgcca atggaatcga aggttgaaga catctgtcag
    31561 ttgatgagtc aaacgcctaa gtgggctgaa ggcttgctac tgaacgctga tggatacgag
    31621 tgtgaattct atcaaaagga ttagtcaatg tggagtcaga aatggctcca tatttttttc
    31681 aaattttttt atcaaaaata aggttcgaat tgggttcttt cttcgcttat agacgaaggc
    31741 aaaaaaatca aaaataaatt cgagtcagag tttactttcg accattttct tcgcctgtaa
    31801 gttagaaggg gcaaaatgcc tctcctgaaa attctattac aggaggttca caagatgaac
    31861 gaattgaaag tttttgaaaa tgcggagttt ggctctgtac gcacggtgat gattggcgat
    31921 gtccctcact ttgttggtaa agatgtagca tcaattcttg gctacacaaa tccacaaaaa
    31981 gcattacgag atcatgttga tgaggaagat aagacggtga acgaatcgtt cagcgttaat
    32041 gggacaatgg ctatccttat caacgaaagt ggattgtact cactcatcat ttctagcaag
    32101 atgccgaacg cgaagaaatt caagcgttgg gtgacaaatg aggtcttgcc gtctatccgc
    32161 caacacggtg tgtacgcgac agaagatttc atcacaaagt caatcgaaga tccagcatgg
    32221 gccatttcag tgttgaagca gctacaagag aaaaaagaaa tggtggcgat gcaacaacaa
    32281 atgattttgg agatgaaacc aaaagttagc tactacgatt tgattcttca aaacaattct
    32341 gtgatgtcga tttctaaaat ctcgaaggac tacggtatgt caccacaggc aatgaacaag
    32401 ttgcttcatg aactgggaat tcagtacaag caaggcaaga tgtggttgct ttatcagaag
    32461 tatgccaatc aaggctacac acaatcaaaa actcatgcga tagatgcaga gcgcagcgtg
    32521 atgcatacct actggacgca gaaaggtcgt ttgttcttgt acgacttgct gaaatcgaag
    32581 aagaacatct taccagtgat tgagcgaggt gataataatg cctagacgta aagattacag
    32641 aacatcagtc tatgtctgct cgccgttctc aggaaatgtt gtcgtcaacc ataaaaacac
    32701 taagcgttac tgccgtattg ctcttgaccg tggctacatg ccagttgcgg cacatctgat
    32761 gtatccacaa ttcatggacg acgcaacgga gcgtgatgtg gcaatgcaca tgtcacttgt
    32821 tctcatgggc aaatgtgagg aagtctgggt ggtgggtgac accatctccc aaggcatggc
    32881 agttgaaatt gaacaagcgg agtattggaa gaaacatatc aggtacttcg atgatgagat
    32941 gaaggaggtc tcaaatgctt aagatgacgt tattcacatc caatcaggtg ggtaattcca
    33001 agaatggatt gttcccaacc aagcaagtgg tgacagataa agaaacactt gttgccacgg
    33061 tcaagctgga tcatgtgatg gctgagtaca aaggcaacta tcgcaaaggt gacaacttca
    33121 tccagtctga tgtgattcca cttgattgcg acaatgacca ctcagacaat ccagatgatt
    33181 gggtagcccc tttcgaagtg gcgatgcagt ttcctgatgt gacgtttgcg gtcagctatt
    33241 cacgtaacaa catgaaagac aaaggtggca aatcagcacg tccacgtttt cacgtatatt
    33301 tcccgattcc tgccacaact gataaagatg aatatgtgga actcaagaag cagattcaaa
    33361 gtgagttccc atactttgac aacaatgcga tggatgcggc acgtttgctt ttcggaacac
    33421 caaactctga agttgaaatc tatgaaggta gtaagttgat cactgatttt ctcgaagatg
    33481 atctgtttgc tgattgggat aatgcgcagt cagagattag cgaaggcagc cgtaattcaa
    33541 gcatgagtca catcgcagga aagctcatca aacgctacgg tgcaacggaa gatacgtatc
    33601 acaagtatct cgaacaatcc caaaaatgta atccgccact gccggaggag gagcttaaag
    33661 ctatctggaa tagcgcggtc aactttggga agaaagtgaa acaacaagaa ggctacattc
    33721 ctccagagca gtacaactct gatttatcac ttgagccaac cgactactcg gacttaggtc
    33781 aagcaacggt cttagctcgc gagtatgaat gtaagcttca ttactcaccg tcaactggtt
    33841 accttgtcta caacggcagc tattgggaag agtcgaaacc aaaagcgcaa ggtgtgatcc
    33901 acgcattgac agaacgtcaa ctggaagaat ctgaaaccga aattgagaag cgtaccaagg
    33961 aaatggttag taatggcgcg ttcgatgtac ttgctagtgt tggtccgaaa aaggcagtca
    34021 ccatgttcaa cacagcgcaa cgtcatagtt ttgatttgta ccaacacgca caagagtata
    34081 agaagtttgc tgtcaaacgc cgcgacagca agtatctctc gtcagcatta accgaggcaa
    34141 agcccatgct tgaaatcgag caacggttac tagatgtgaa cgagttcttg ctgaacacac
    34201 caacagcaac ctttgatttg cgaacaggca aatcacaaga ccacaactca gaagattaca
    34261 tcacgaagca aacggaatgt gccccgagtg acgccaatca gcagatttgg ttggacgctc
    34321 tgaacaccat tttcgtaggt gaccaagagt tgattgatta tgtccagatg attgtaggac
    34381 ttgcagctat tggtaaagtt tacgttgaag cgcttatcat ctcgtatggt gaagggcgta
    34441 acgggaagtc cacgttctgg aatgtcatca gccgtgtgct cggtaattac tctggcagca
    34501 tctcagctga tattcttaca agtcaaatcc gacgcaatgt gaaacctgaa cttgcggaag
    34561 cgaaggggaa acgcctactc atcgcagccg agcttgaaga ggggatgcga ctcaacacat
    34621 ctaacattaa gcagctctgc tcgacggatg aaattgcggc tgagaagaaa tacaaagacc
    34681 cattcaagta tgtcccaacg catactttgg tgctctatac caaccacctc ccaaaagttg
    34741 gagcgattga taaaggaacc tggcgtcgtt taattgtgat tccattcctt gctacgattg
    34801 acggcaataa ggacatcaag aattatgcgg actacctttt tgaaaatgcc ggcggagcca
    34861 ttttgcaatg gatccttgac ggtgcgaaaa aagtcatcgc cgcagacttt catttaacgg
    34921 ttccacaagt tgtcgctaat gcaatttctg aatataagac ggcgaatgat tggcttggcc
    34981 atttcttaga cgagtgctgc gagactgctg ttgactttga agaaaaatct ggtgagttgt
    35041 atgcagagta tcgtgcgttt tgtacgcgaa ctggtgagta cattcgtagt tcagccgatt
    35101 tttacacggc tctggaggcg ggcggattta agcgccggcg aactaataaa ggaaacaaga
    35161 ttgttggttt gcaactcaaa tccgagttct tggaatagtt gattttaaag gaaagtgtaa
    35221 cctgtgtagg tcttatatag aacttttctt aagggataaa aaattaccac ctatataaag
    35281 ttatgtattg cgtattcacg ggttacacca tacttgcaga ttggagaata tctaaatgaa
    35341 atttaaacag tggttacaaa ttggaatcgg taaacgtgat ttacgatttg atgagtttta
    35401 cgagtttgcc atgtcggatg tcacgtatcc ttggaagaaa tcattcgata agcaagtcgc
    35461 acatctgatg attcactgcg cagaaaagga atacttggaa atcttgcgtg atgcgtattt
    35521 cgagtatttg acggtgtggc tatgagagaa aagcaggttg agcaagcact agtgaaagcc
    35581 gtcaaaaggg taggtggtat ttgcccgaag tttacatcac cggggcttgc tggtgtgcct
    35641 gaccgattgg tacttatgcc aaatgggaaa cttggatttg tcgaagtgaa ggcgccaggg
    35701 aagaagccac ggtctttaca gctttttcgt atgaaacaac tgacagattt agggtttcag
    35761 tgtttcgtgc ttgatgagat tgatcagata cctgagttac tagaaagaat aggaggtgat
    35821 gtctgatgaa gttcgtacca cataactacc agaaatatgc gattgatttt atcaaagatc
    35881 ataaaactgc cgcccttcta ttggacatgg gcttgggtaa aacggtgaca acgctgacag
    35941 ctattaaaga cttgatgcat gatgacttta caatacaacg tgtcttaatt attgcaccgt
    36001 tgcgcgtgac gcaatcaaca tggccaactg aaattcaaaa gtgggatcat ttgaaagatt
    36061 tatcgtattc cgtggttctt ggaacaccaa gtcagcgaat ggcagcatta caaaataatg
    36121 ccaacatcta tctgatcaat cgtgagaact tggactggct gattaacaaa tcgggtttta
    36181 cgtttgattt tgatatggtg gtgattgacg aactgtcgtc cttcaaaaac ttaaaagcta
    36241 aacggttcac tagctttatg aaagtcagac acaaggttga ccgaattgtt ggattgaccg
    36301 gaacaccttc aagtaatggt ttgatggatt tgtttgcgga gttcaaggtt ctggatatgg
    36361 gcgagagatt gggctattat atttcacgtt atcgagataa atactttcta cctgacaagc
    36421 aaaatggcat gcagatttat tcttggaaac caagggaaga tgcagaacaa gaaatctacg
    36481 acaagatttc agatatcacg atttcaatga agtcagtcga tttcttggat atgccagagc
    36541 ttgtgatgaa tgaagtgcca gttgatatgg gactcaaaga aaagcaaaag tacgatgaaa
    36601 ttaaggcaga cttggtgcta caattgaaag aagccaccat tgatgcagcc aatacagcgg
    36661 tgctttcgaa taaactgcta caaatggcga atggagcaat ctatgatgag tttaatgtga
    36721 gtcatcacat ccatgaccag aagttagatg ctttggaaga tttgattgaa ggtacaaatg
    36781 gcaaaccgat tctgattgcc tattggttcc agcatgatct ggagcgtatc aaggaacgat
    36841 tcaacgttcg acagatcaaa acgtctcagg acattattga ttggaacgaa ggtagcatcc
    36901 ccatcgctgt cattcatccg gctagtgcag gacatggatt gaatttacaa gctggtggtt
    36961 caacacttgt atggtttggt cttacgtgga gtttggaact ttaccaacaa accaatgcaa
    37021 ggctgtggcg acaagggcaa agtgatactg tggtcattca tcacattatt gcgaaagata
    37081 cgattgatga agatgtgatg ttggctttga aacgaaaaga taaaacacaa tcatgtttga
    37141 ttgatgcagt gaaagctaga ttggagggaa gcaagtgaca ccaaaagaat acttgaaaca
    37201 agcgtaccgt ttgaataaac gaattgacga ccggattaaa gagaaggcgg agctctatca
    37261 actggcggtg acagtttctt ccacaacatt cgaagaaaag atttcaagtg gcacacggaa
    37321 tactgaggca ccatttgttc gtaaccttga gaagtgttac caattggaag ctgaaattga
    37381 tgcagagatt gataagtttg tagacctcaa acgacaaatg cgtggcgtga ttgatgaact
    37441 cacaaacgtg gacgaaaaga ttgtgttaat caatcgatac ctgttggaag agagctggga
    37501 caagattgct gaagatcttc acgctgacgt gcggacagtc tttcgttggc atagcaaagc
    37561 tcttgagaac ttgaagctac cagatgaacc catagtcatt tgaaatgtgt cagtgaatgt
    37621 cagtagcctt tagtgatatc attaagatgt caaaaaagac aaacgatact caagcaatac
    37681 cagcaataga agtatcgaac aaatactcga agcctttgtg ggaaaaatcc ttcaagggct
    37741 tttcttttgc ccgaaaggat gtgaccccac ttgccaatga aaccagctaa cccctgtaag
    37801 ttcccaggtt gtccgaaccg tacgcatgat acttattgtg atgaacataa aacacttgcg
    37861 aggaaacgtt acgagaaata cgagcgtgat ccggcaatca acaaacgtta cggcagagcg
    37921 tggaagaaaa tacgggcaag gtacgtggca gcacatcccc tatgtgagat gtgtctgaag
    37981 gaaggacgct ttacttcggt tgagattgtg catcacatca aggaattaag tgatggtggt
    38041 acgcatgatg aaaataattt gatgagtgtc tgcaaatcat gtcactcgaa aattcacatg
    38101 actcgacgaa acacaaaaga taaaacaatc atgtgaaaaa taacaagggg gggaggggcg
    38161 gtaattatcc ctaaaccctt ctaaactgta cagcgcgcta ggcccttacg cgaaaaaagt
    38221 tcagttcaaa cgggggatag ccccaaacaa gaaaaagagg tgataaattt tggcaaaaga
    38281 tggaacacaa cgcggtggca gacgacctcg ttcaggggaa cgctcacagc cactcgttga
    38341 taaagttgct gctggaaaga aagcatcgcg gatggaattt gacttaccgg atgttgaatt
    38401 attaattggt gaagatattg gtgagggtgt agagcttgag ggcaatgatt atcctgaacc
    38461 aagtgcttac ctttctgcaa aacagaagga tggcactgtg ctaggcgctg atgaaattta
    38521 taactcaacg ttccaatggt tgaaagaccg cggatgcgaa acgttgatag ccccacgaat
    38581 ggttgagtcc tacgctcaga actttgcgcg ttttgttcaa tgtgaaaatg ctctgagtca
    38641 atatggtttg ctcggaaaac acccgactac caaggcagtc atggcctccc catttgtggc
    38701 gatgtcgcag aacttccaaa agcaagcaaa tgtgacgtgg tatgagattc aacagattgt
    38761 aaaagagaat gcatcagtgg aatacacagg agatccgaat acggatgcga tggaacgatt
    38821 gttacgttct aagaatggtt gaaaggaaca gaatttatga tagagaaggt taatccgcaa
    38881 catccagata aagttgcaga tcgtattgcc ggagccattg tggacttggc atacgcagaa
    38941 gaaaataatc caaagatagc agttgaggtt ttaattggtc atggcatctg tcatgtgatt
    39001 attgaaactt caactgcttt aaatttgaat gaaatcagcc aagcgattaa tcgtatcgca
    39061 ggcaaaatta aaaaagatgt ggtgattgtg ccccaagata aagagttggc tgacaatcaa
    39121 gcgactgaaa ttcgttgtgg cgataatgga atctttcgtg ggatgccatt gactgatgaa
    39181 cagttacagc tctcaaaaat tgcgcgtgac ttatatcagg accatccatt tgatggaaag
    39241 tacattcttg atggtgaacg cttgataatt tgtcagagcc acgcaaagac aaatgagtta
    39301 aaacaaaaat atccaaacgc tacagtcaac cctctaggtg attggactgg cggcccggat
    39361 gtggatacag gtgcgactaa tcgcaagctt ggttccgata tggcagacgg agtaacaggt
    39421 ggcggtatcc atgggaaaga cttatcaaaa gctgatgtgt cagtgaacat ctacgcattc
    39481 ttgaaagctc aagaaactgg tatgcccgtt gagttaagct gtgcgattgg tgacactgag
    39541 attgatggcg tgccttattc tgaaatcgtg gaagttgcgc gtgattatgt gaatgagctt
    39601 ggcgggtttg agaaactagc agaatggggg ttgttctaat gaacacaaca gaacgttttg
    39661 agaaagttga tattgataag ctggtccctt atgccagaaa tgcccgtacc cacagtaagg
    39721 atcaagtgaa acagattcaa gcttctatgc gtgagtttgg gttcataaat ccggtgattg
    39781 ttgataagga ttataacttg attgcaggtc acggacgtat tcttgcggct aaagaagaag
    39841 gtatcaagca ggtaccgtgc gtgtttgtgg aacacctaac cgaggcacaa aagcgtgcgt
    39901 acatacttgc tgataaccga ctagcaatga acgccggttg ggatgatgaa atgttggcgg
    39961 ttgagttatc agacctgcaa ggtgaagagt ttgatttgga tttgcttggt tttgaaactg
    40021 atgaacttga taaattactc aacggtaatg atgatgccca agatgatgac tttgatgtag
    40081 atgaggaact tgaaaagcca gccttcagta aaacagggga cttgtggttg cttggtcgtc
    40141 accgtttagt ttgtggtgac tcaacaaaac ctgaaactta tgagaaactc atggacggca
    40201 aactagcaaa tcttgtggtg acagatccac cttatggggt ctcatatcaa ggtaaagcgg
    40261 gaacgattaa aaatgataat ctgaaggacg aggcatttta caacttcttg tatgatgcct
    40321 tttcttgtat ggaaagtgtg atggccaacg acgccagtat ttatgttttc catgcggata
    40381 cggaaggctt gaacttcaga aaagcattcg tggatgcagg gttctattta tcaggtacgt
    40441 gcatctggaa gaaacagagt ctagtgcttg gtcgttcgcc ttaccaatgg cagcatgagc
    40501 cggtgctcta tggctggaag aagaaaggca aacatcaatg gtacaccgga cgtaaggaat
    40561 cgaccatctg ggaatttgat aaaccaaaga agaatggtga ccacccaacg atgaaaccaa
    40621 ttccgctgtt ggcatatccg attaccaatt cgtcactcag caactgcatc gtgttggata
    40681 gttttggtgg cagcggatca actttgattg cttgcgaaca gaccgaccgg atttgccata
    40741 cgatcgaact ggatgaaaag ttctgtgatg tgattgttaa gcgttatatc gagcaggctg
    40801 gaagcgatgc agacgtttca gtgattcgag atggaaagac tatcgcattt aatgacctag
    40861 aaaatcctga ataaataacg tatcacttgc tataaaatcc ctttagagtg atatatagac
    40921 taccaaaaga atacggaaga caaagttatg tttccaactc atgaacaaat cgagggtatg
    40981 aaaaaacgtt accctgaagg tagtcgtgta gaactcatca agatgaacga cccacatgcc
    41041 ccagcgaagg gcacacaagg cacagtaacc ggtgttgacg acattggttc aatccttatc
    41101 caatgggaca acggttcaac actgaacgcg gtatacggcg aagatgtggt gcacatcatc
    41161 aaaccagact tcgaattggt ctttcaaaac ggtagaagag aaggatttga aaccttcaat
    41221 gatgcttggc aatacgtgac ggacatgata ttgaaccacg atttggtctg ggttgacctt
    41281 gagtcaaaaa cagaagatac catctacatc agaaaggggc tttaagatga aagtgagtaa
    41341 acgtaagatt tacaacatcg caaagaatta catcgttcag ttgcctgaac gtggcgacct
    41401 gaaggcccac aacagcgacc gtgaagactt cttggacatt gcggtttggt gtttggaaga
    41461 tgccttaatt gcggcctatg aggcaggcag aaaggacggt caaaatgaac gcaaagatta
    41521 agcaacaaat cattgagatt gcagaaagtg gtctgacaaa tatgtttgat gcgattgcag
    41581 tgcagcgaat tgcacatgac cggcactttt acgagttggt gattttcatt gaagaacaca
    41641 aagatgagta cagccgcttt atcttggagg gtgataaatg atggattatc aaatttacat
    41701 gccagataat gaagatggca ttgaagaagg atactacgat tggcaagaac tggtgcaatt
    41761 gctgcgggat aacaagaaca accctgaagc cattcagttc attgccgata tgatggaata
    41821 aagagattaa ggcagctgac aacagctgct ttttgcgtag aagggagtga gttccaatcg
    41881 ataattacaa accaactgaa tttatggaca aagactccca ttatgataag tcacgtgcag
    41941 attatgccgt ggcctttatt gagttgttga aacacacgaa aggtcagtgg ttcgggaaac
    42001 cgttcaaact gatgccttgg caagaaaaga ttatccgtga cttattcgga acgattaagg
    42061 caaatggcta cagacaattc aatacggctt atgttgaaat acctaagaag aatggtaaga
    42121 gtgagttagc ggctgccgtg gctttgcttt tgacttgcgg tgatggggaa gaacgcgctg
    42181 aggtatatgg ctgtgctgct gatcgagcgc aggcttcaat cgtatttgac gtagctgttg
    42241 atatgattcg ccagaaccca gcgttaagca aacgagtgaa aatcctgact tcacaaaaac
    42301 gaattattta tcagccaacg aatagtttct atcaagtatt atcagctgaa gcatactcta
    42361 aacacggatt aaacagttct gcggttgtat ttgatgagct tcacgcacaa cctaacagaa
    42421 agctgtttga tgtcatgacg aaaggggcag gggatgctag aacgcaaccg ctattctttt
    42481 tgattacaac ggcagggaat gataccaatt caatttgtta tgagcagcat caaaaagctc
    42541 tcgacattat tgaaggtcga aagcatgata aaacattcta tccgttaatt tacggtatca
    42601 agcaagatga tgattggact gatccagaag tttggaagaa agccaatccg tcacttggga
    42661 taacgattgc agaagaaaag gtggtggctg cttgtgattc ggctaaacaa aatccagctg
    42721 aagaaaactc attcagacag cttcgcctga accaatgggt gaaacaagca gtgcgttgga
    42781 tgccaatgga taaatgggac gcttgttcat tcaatgcaga tgaagaaagt ttgagaggtc
    42841 gagtttgtta cggaggtctc gacctttcca gcacgaccga ccttacatcc ttcgttttgg
    42901 tgtttccacc tgaagatgag gatgacaagt ttgtgatact gccatattac tgggtgccag
    42961 aagatacctt ggacttacga gttaaacgcg accatgtgaa ttatgatttg tgggccaagc
    43021 aggggtacat acaaaccact gagggaaacg tagtccacta tggtttcatt gaaaaattta
    43081 ttgaggaact gggtgagaaa tacaatatcc gggagattgc ctttgaccgt tggggagcag
    43141 ttcaaatggt tcaaaactta gaaggtatgg gatttactgt agttcctttc ggtcaaggtt
    43201 tcaaggatat gagcccggct actaaagagt tgatgaagtt gactcttgaa cagaaattag
    43261 cccatggcgg tcatccggtt cttcgttgga atatggacaa cattttcgtc cgaactgacc
    43321 cggcaggtaa tatcaaagct gataaagaaa aatccactga gaagattgat ggtgccatcg
    43381 cgacaattat ggctctggat cgtgcaattc gttgtggaaa tgataatggt gccagtgttt
    43441 atgatgatag gggattgttg tttctgtgaa atatgagctt ttattacaat ccattgatat
    43501 aattgtcttt ggaggtgtat gtgtatgaat gaatatttga ttcaagtagc accagataaa
    43561 atatcttcag acccggacaa cacattaatt tctagactgt tttatagaaa atggtgggga
    43621 aaaattctaa ctgaagattt gacaaagtat gctgaaggaa ttgggaatta taggggagaa
    43681 acgattattt ctttcaacac aattgctggt gcaacgatac ggttattgaa atgctataat
    43741 caaaagaact tttcaatgcc agatagtcat tatgggcgtt tgaaagtcat tttatattcc
    43801 gatgatatca ccgaagagat gaaagtgaaa tttgtaaaat tctatgattt aagtcatact
    43861 cttgcaaatt ttatgccact atatgtatca aaaccttctc tcaatggtgt taaaggtaaa
    43921 gggccgtatc atgactttcc tgatttattt tattctgatg taagagataa acgatatggt
    43981 tcatcaacta acgctgataa atttatcgtt aataataaag actatttctc taaattcaaa
    44041 gattggaaaa gttttataga actaaattat ttacaagatt tcttcgatga tgaagattat
    44101 atgacgttta aaaaacttgc accaagtagt tcattctttc cttatcaaag aaaatcctta
    44161 acacctgacg aaaaatcgaa atgtttagag gaagtaaata cctttttaaa taatgcaatt
    44221 gagataattt tgtcaagagg aagtcggttt tatatctaat gtgaacacag catctacaaa
    44281 tcgtaggtgc ttttttgata ctcaaatttt gaaaggagca atcaatgggc ataataaaat
    44341 cactattcaa atcacgcgat aaaccaacaa atcaaaccat catgaatcca taccgattta
    44401 tgtttggcgg ctcatcagct ggtaaaagcg tgaccgaaca aacagctatg cagatgacgg
    44461 ctgtgtactc atgtgtgcgc gtgctatcag aagccgtggc aggattgcca ttacactttt
    44521 accaataccg agatgctggc ggcaaagata aagcaatgaa acattcgttg tatttcttgc
    44581 tgcatgatga accaaattcg gaaatgacat catttgtatt tcgtgaaacc ttaatgactc
    44641 acctgctact ttggggtaac gcctatgcgc aaattatccg aaatggcaaa ggtgaagttg
    44701 tagctttgta tcctttaatg ccagataaga tgacggttaa tcgcgatgac aacaaggaaa
    44761 tctattattt atatcaagct gatgtcgggc aagtcagact ttcaaaagct gacgtgcttc
    44821 acattcctgg ccttggcttt gatggcttgg ttggttattc accaattgcc atggctaaga
    44881 atgctatcgg gcttgcgatt gcaacagagg aatatggttc taaatttttt gccaatggtg
    44941 ccaatcctgg tggtgtccta gaacatcctg gaactttgaa agacccagga aagcttcgtg
    45001 aaagttggaa tgcgacattt ggtggcagtt caaatgcggc caaggttgca gtacttgaag
    45061 aaggcatgaa gtatacgcct atttccatgt caccagaaca agcccagttc cttgaaacac
    45121 gtaagtttca actggaagaa atcgcacgga tattccgagt gccccttcac atggttggcg
    45181 acttagacca cgcgaccttc tcaaacgttg aacatttgag tttggatttt gtgaagttct
    45241 cattatcacc atggctcaat cgttgggaac aagcacttaa taaatcactg ttaagtcgcg
    45301 atgaaaaaga aaagtatttc ataaagttta atgtggatgg cttattgcgt ggtgactatg
    45361 ccacacgtca acaaggctat gcaaccggta ttcaaaatgg gttcctatct cctgatgatg
    45421 tgcgtgaact tgaggacttg aacccaatcc ctggtggtgc tggtgcgaaa tattacttca
    45481 atggcaatat gctgccaatt gaacaagccg gggcagctta tggcgtaaac aaagaagaaa
    45541 gcgaggaaca agatgaagaa gttttggaag tggaagaatc aaacggttct gaatcaagac
    45601 aaccaagagg aaatcctaga gagaaccctc tacctcaacg gaccaatcgc tgaggagagc
    45661 tggtttgatg atgaagtcac accgcagatt ttcaaagatg agttaaattc aggtggaggt
    45721 aacgttacag tctggattaa ttcgggtggt ggcgattgtg tcgctgccgc aagtatttac
    45781 aacatgcttg ctgactacaa aggtaaagtc acagtcaaga ttgacggcat cgccgcttcg
    45841 gccgcaagtg ttattgcaat ggcaggtgac aaggtactca tgagtcctgt ggcgatggtg
    45901 atgattcaca atccgtcagc ggttgcgttt ggcgaaaaag ttgatatgga gaaagccatt
    45961 gccatgcttg atgaagtgaa agcaagtatt atcaacgcct atgaacgtaa aacgaattta
    46021 tcgcgtgtgc aactggcaca tatgatggat gcggagacgt ggatggacgc aaataaagct
    46081 gttcacctag gatttgccga tgaaatcatc ggtgagattg acccggatat tccggttaat
    46141 aattcgatgt actcggaagt caaagtaact aattcactga cttcagcgat tgctaagaag
    46201 tgtcgtattg aaccacctga accaaaggaa cgagaagaaa ttaataacac tatcccagcc
    46261 gattcgttaa tgagtcggct taatttaatg aaaaattgga ggaactaacc catgaacaaa
    46321 attcaagaat tgatggaaaa acgtgcacaa gcttggaacg gagcaaaggc ttttgtagaa
    46381 gcaaagcaag ataaagatgg cttgatgtct gaagaagatg ccaagactta tgcggaaatg
    46441 gaaacaaaag tgaagaactt tacgatggaa attgaacgta tgcagtcaat ggaagcgatg
    46501 gatcgtgaac ttgaaaagcc aatgagccaa ccattaactt cgcaacctat gactactgaa
    46561 aataaaatgg aacctaagaa aacaggacgt gcttcagctg aatataaaga aggtgtactt
    46621 gaagccttgc gctcaaactt ccgacatgtt tcaaatgttc ttcaagaagg tgtggatacg
    46681 gcaggcggtt atttggtgcc agaagaatat gactcacgtt tgattgacgg catgacttct
    46741 gaaaacatca tgcgtacact tgcgacaaca attacgacaa gtggtgaaca caagattaat
    46801 attgcaggtt ctaaaccagc cgcttcatgg attgaagaag gtggcgagtt gagctttggg
    46861 gatgcaacgt tcgatcaaat cttgcttgat gctcacaaac ttcatgtggc aatcaaggtg
    46921 actgatgaat tgctttatga caacgccttt aaccttgaaa actatatcat cgaccaattc
    46981 accaaagcct tagcaaacgc ggaagaagat gcgttcttga atggtgacgg tgttggtaaa
    47041 ccactcggta tttttgcagc aacaggtggc ggacaagttg ccattacaac taatactcaa
    47101 agtgcagtta cggcagatga tgtggtgaac cttgtgtatg ctttgaaacg tccatatcgt
    47161 aaaaacgcga agtttatcat gaacgaccaa accatcagct tgcttcgtaa gttgaaagac
    47221 aacaatggtg cctacatgtg gcagccagct ttggtagcag gtgaacctga ccgtctgctc
    47281 ggttatccag tttatacttc ggagtttgtt ccaaccgtta aggcaggtgc gccagtcatt
    47341 gcttttggtg atttcagcta ctataacatt ggcgaccgtg gcgtgcgttc attcgaccag
    47401 ttgcgtgaac tctttgcagg taatggcatg gttggtttct tagctaaaga acgtgtggac
    47461 ggtaaacttg tgttacctga agcagtgcaa atcttgaaga tgaaagcatc tgcctcatct
    47521 taaggagtag cctatgagtg acttactaaa taaggtcaag gcaaacctca tacttgagca
    47581 taacgaagat gatgagctgt tggaacgatt gattctgacc gctctttctt atgctgagag
    47641 ctatcaacat attgagtctg gctactattc agaacatgaa atgccgccaa ccactgaaca
    47701 ggcggttatt gtgttagcca gtaactttta cgaaagccgt gatggttcaa ctggtggctt
    47761 ctttgcggat aacgtcaatg ccagtaagca agtctgggaa acggtcaatc tgttactcag
    47821 gctcgatagg cggtggcaag tatgagtttt ggaaagatga atcaacgaat acaaatcgtt
    47881 aaagtgacga accaaaagga tagcgctgga ttttcagtga aacaagatga agttcttgca
    47941 gctgtccatg catacaaaga ggaaaagaat gcgactgaga aatgggtaaa catggcgact
    48001 tttagttcag cgacctgcct gtttcggatt cgttatattc ctaatctaga ggttacgaca
    48061 gagatggttg tcatttcaga tcagaaaagg tatcagattg tgtcggtgga gaatgtccga
    48121 aatcgcaata tgtaccttga aatgctgact actgaaatgg aggtgagtgc ggatggctaa
    48181 agcattgatg aagatgccag aagatttctt gttaaaggtt tcaaaactag aaaacaagac
    48241 agatgaaatc atccccaagg ttttagaaag tggtgctgaa gtggtggaag gcaaagtccg
    48301 aagtaactta actgccgttg ttggctcaaa tacaaaagta ccaagtcgtt caactggtga
    48361 acttgaatca gcccttggta tcacacaagc tcgtcaagat aaaagcggca actggaacat
    48421 caaggttggt tttgatgagc ctcgaatcga tggcagttca aatgctaaga ttgcgaacat
    48481 tctcgaatat ggaaaacacg gtcagccagc aaagccattc ttgaaacctg caaaatcaca
    48541 aagtcgcaag tcatgcattg aaacaatgaa atcaaaaatg gaaagtgagg taaacggcat
    48601 ttgaacattt tagaggaatt gaacagtctt tttgccacac ttcatattcc tgttgaaacg
    48661 ggcgagttta gtgataaagc gccagacaag tattgtgtgc tgacaccact cgctgacagg
    48721 ttcgatgttt acggggataa ctttccaacc gttgatgtac aggaagttcg catttccttg
    48781 tttgtgaaag gcaactatct gaacacgcaa aaacaactgg ttcagaactt gttgaaacac
    48841 gagtttacga ttactgaacg attatttgta ggatttgaaa aagatacaaa ttactatcat
    48901 gtggcgattg atgtcgccaa gcattatgaa atggaggtct gaaaatggca acgattggat
    48961 tggataaatt atattatgca ccgattacag agtcggcaac tactggcgaa gaaacatacg
    49021 gcacacccgc tatgcttgcg aaagcaatct cagctgagct ttcggtggaa cttgctgaag
    49081 caactttata cgccgatgat ggtgcatcag aagtagtaaa agaatttaaa aatggaaagt
    49141 taacgctcgg tgttgatgat attggacgtg ctgtggcggc agtgcttgtt ggtgcaaccg
    49201 tggatcaaaa cggtgtctta atttcgacaa ccgaagatgg tggcacacca gttgcgattg
    49261 gtttccgtgc tcgtaaatcg aatggcaaat acaaatattt gtggttgtac cgagtgaagt
    49321 ttgccgtgcc cagcacaagc ctagcaacaa aaggtgacga cattacattc tcaacaccaa
    49381 caattgaggg tacagtcatg cgtcgtaaca agttagatgc tagtggtaag catccatgga
    49441 aagcagaagt tgatgaaaac gatacgggag ttgaacaaac aactatcaat ggatggtaca
    49501 cgagtgttta tgaaccagat ttcacagcag actaggaggc aacaatgagt gaagaacgca
    49561 gtgccaagat tagccttggc ggagatgaat atgaactgat tttgacaaca aaagccacga
    49621 aagaaatcgc cagtcactac ggtgggcttg ataaccttgg tgagaaattg ttgaaatccg
    49681 agaactttga attggcgctt gatgaaatca tctggttgat tacgctgttg gcgaatcagt
    49741 ccatcaagat ttacaacttg aagaacacag aaaagaaaga tgaactcacg actgaatatg
    49801 tggagttgat gacgagtccg ttgcagttgg ctgaatataa atcagcaatc actgaagcaa
    49861 tgttcaaggg cacagctcgc aatatcgaaa gtgaaacaga ttcaaaaaac aaagcaggcg
    49921 agtaagtgat gaagaaacat ttactcgcct gtattactat gggacagttc aaatgggtat
    49981 gacgtcagat gatttctggt actgctcgct tggactgttt ttggatttat gggagtgtca
    50041 taagcaattt actgggatta gcaagccgaa agttgagtta ttcattgatg atgtgatacc
    50101 gagtggaatt taagggaagg tggtgattta tttggcagat aattttggtt taaagattgg
    50161 cgttgaaggt gaaaaggagt ttaagaactc gttgcgtgaa atcaaccaat cattcaaggt
    50221 gctaggttca gaaatgaaac tggtatcatc cgaatttgat aaaaacgata aaagcatcag
    50281 ctcactgtct gctcgcaacc aagtcttaaa caaatcgatt gatgcccaga aagaaaaaat
    50341 caccaccctt gaaaatgctt tgaagaacgc tagtgacagt tttggtgaaa acgataagcg
    50401 tactcagaat tggacagtgc aattaaacaa tgcaaaagcc caactcaata atatggagcg
    50461 tgaagttgct aacaacgaga aggcaattga cgccatgggt actgaagaag ttgatgctgc
    50521 taagaaaact gatgagcttg gcaacgaact caaggataca ggtgacgagg ctgaaaagtc
    50581 tggaagtaaa tttgaaaagt tcggtggcgt tctaaaaggt atcggggtag caatgggtac
    50641 agtggcactt gctgctggcg ctgctgccgt ggagctagga aaacaagtgg tatctgctta
    50701 cgcggactat gaacaattag tcggtggggt ggatactctt ttcaaggatg cgtcatccac
    50761 cgttcaaaag aatgccgaca atgctttcaa aactgccgga atgtcagcca atgaatacat
    50821 ggaaactgtc acaggtttct cagctgcctt gatttcatct ctcggtggtg ataccaagaa
    50881 agccgcagga gttgcggatc aagctgttac tgatatgagc gataatgcaa acaagatggg
    50941 gacggatatt gagtccatcc agaacgccta tcaaggtttc gccaagcaaa attacgggat
    51001 gctggacaac ttgaaattag gttttggggg cacgaagtcc gaaatggaac gtttgctcca
    51061 agaggctgag aagatttctg gtgtcaaata tgatatctcg aacttcgctg atgtgactga
    51121 agcgattcac gttattcaaa ccgaaatggg catcactggt acaaccgcca aggaagcgag
    51181 tgagacgata agtggttcga ttgacggtat gaagtctgca atctcaaact tgatggctgg
    51241 acttggtaac agcagcgctg acattggtat gttgattggc aatgtggtgg aacaatttca
    51301 aaacgtcatc aagaatattc taccggtcat tcaaaatatc gtgtcggctt taccggcagt
    51361 ggtggatgcc attattcaag cattggggca gttattgcca acactcttag aagcagtaac
    51421 ctcattattc tcacaagtgc ttgaaacgat acttggctta cttcccacac ttattccagt
    51481 ggtggttcaa gccgttttaa cgattgttca aacacttatt gagaacttac ctttactgat
    51541 tgacgctgcc tttcaacttg tgacaacact tatccaagga ctaggcgaag cgctgccaga
    51601 acttattcca attacagtac aggcaatcat aacgattgtg aacggcttga ttgaaaactt
    51661 gccattactg ttggatgctg ctttacaact tatcatggga cttgcccaag gacttatcac
    51721 agcacttcca atactgattg aaagtctgcc acagattatt acaggaatcg tgaactttat
    51781 tatcagtgct attccacaaa tcattcagac tggtattcaa cttttgacat cacttgtgac
    51841 agctttgccg gatatcatca atgcgattgt tgcagctatt ccagtgatta ttcaaaatat
    51901 catcaatgcc gtgattgggg caatccctca gttgattgac gcaggtattc agttacttgt
    51961 ggcattgatt ggtgcgttac cgcaaattat cacaacgatt gcgaatgcca tgccgcaaat
    52021 tatcaacgga ataacaggaa ctttggtaaa caacatcgac aaaatcatca atgctggtgt
    52081 gaagttgctt gtggcattag ttcaaaactt accagcaatc atttctgcca ttgtcaaagc
    52141 agtaccgcaa atcatcacag ggcttgcgaa tgctttcggt gagtatgttt caactatggg
    52201 aactatcggg aagaacttga ttacaggatt gtggaatggt atcgctgatg ctggaagttg
    52261 gttacgcaac aagatttcag gattctttgg cggtgtagta gacaacatca aggacttctt
    52321 tggtattcat tcaccatcaa cactgtttcg tgatgaaatc gggaagtaca tggcacaagg
    52381 gattggcgta ggttttgata aggaaatgac tggtgtttct aaaacgatgc agaaagctat
    52441 tccaacaaaa ctttatatgc caagctttga tattgatacc gatatccacg ctgccgttga
    52501 tagcacattc tcgatgcttg atttaggtgc taaggttgat gccttaacaa acatcacgag
    52561 tgaaatgttg ccaacgctac ttaaagcaat ggacattaaa gtcgtcttga atgatggaac
    52621 attggtcggc aaacttgctc cagaaattga taagagcttg gcgacgttga atcgtagacg
    52681 tgtagcatat tagtgttgga ttttcacatt ttgactgtat actataacta acgagtgttt
    52741 actgaagaga tggtggtaat catatgttga agcataaatt taaagcaaag aaacctgcac
    52801 tgataggata tattctactg cttgctattg gattattttt aacggcagta agatggtata
    52861 gcggcataaa tcatggtttt gtattcatca acaatcagat taattctcac atttcaaact
    52921 tcgcattaag ctttcttttc tatttggtga taggctatct ttggttgctt atgggaacga
    52981 atttaagggt tcttactgcg atgggagctg gtctgattgt tgcaaatatt gtatgtgaaa
    53041 cgatgatgac ttttctgaat acaccagatc taatcgatgc aatatatgga atttccggcg
    53101 tgctggttgt atacgcgtat ttagcgatta ccaagaaaat agggctggaa gaaacaaccg
    53161 aataggatga tagattataa actttaggat tagacattgt tgtctggtcc ttttttagtt
    53221 atttagatgg gcggtggtca attgaacgca tttattttag acggaacaat taattcacgg
    53281 actgatttag gtttgcgaat tacggaagtg ccagttattc cagtgacgga acgtgtcatt
    53341 gatagtattg agattgatgg acgtgaagga gatttgacca ttctgaaagg ctggaaagat
    53401 gtgactttcg gttttaaggt agtgatttgg aaatctgatg tttggactag ctggcagata
    53461 attttgccac aaatcctaaa cgctaaaact atcgcatttt caaatgaacc aaacgtgttt
    53521 tacccaatca agtatgctaa ggcgagcgga ctaactcaga ttttatcttc catgtgggag
    53581 tttgaaatgg agttaacctg ttcgcctttt cgttataaga ccaatgtagc gactatcaat
    53641 cgaaccacgt caggaacggt caataatccc ggcaacattt actcattgcc gaagattaag
    53701 gtctacggca atggaactag gacactcacg ataaatggca agcctatcgt gttaaatctg
    53761 acgaatggca acctgacact agattcggat ttgaaagaat gcttttacgg ggatgtggca
    53821 gccaatcaat acatgacagg tgactttcct gagtttcgag tcggcagtaa tactgtgact
    53881 cttggtacag gtataaccaa agtagaaata gaaccgaggt ggcgatactt atgattacgc
    53941 tttacaacaa aacagaatca aatttcaatc ataacggtct cgctgtcctt gatggcaaca
    54001 ttctgaaccc cgtggtgagt gaggaattga acggactctt ttcctttgaa ttcgattatc
    54061 caattcatgc tccacacagt gaggaattgt tgccagaaat gattgtgaaa gcaccagtac
    54121 cagaactgtc cgaccagctt ttcaggattg ttgaacgaga tgatgcactt ggtggttttt
    54181 tgcacatagt agctcatcat attttctatg acctagccac aaactttatt gaagatactt
    54241 atatcgtcaa caagaacggg tcgggagctt tgacacaact gcttggtgca actcaattca
    54301 tgcaccgttt taccggtaca tcaaacatca gtgcggtcaa taatgtccgc ttggtgcgac
    54361 taaatcctgt agaggtgttg cttgatagtg acctagagaa tggttttcaa agcagttatg
    54421 gtggtgaaat tgtacgcgat aacttcacga tttcaatgtt aacgactcgt ggcagtaaca
    54481 acggcgtaca aatccgagac aagaagaatc tgacaggata taaagctgat gttgattact
    54541 ctggaattgt tactcgcatt atgccacaag ggtatgatgg actatttctt cccgaaaaat
    54601 acgtggacag tccaaacatc aaccaatacg tccagcctaa aatcaaagtc atcaagtacg
    54661 acaacgtcaa agttggtgat gaagaggatg agtttactca agccgaggcc tatgatgaac
    54721 ttcgcagatt ggctgggtta gaatactcag ctaacaaggt cgatttgcca aaagccacct
    54781 atgatgtgga gtttgccccg cttgagcgaa ctgaagaata taaagatttt gcacagcttg
    54841 aaacgattaa tttgggtgat actgtttcag ttattcatgc cgaagatgga ttgaacatca
    54901 cagctagaat ggtctcatac aaatacgatc catttctgaa agcgtatatc tcagtaacac
    54961 tcggaaacat cacgccaaaa ttcacggatg tggcgaagga catcaaccgg gtggacaaca
    55021 aggcggagca agccaaagac gatgccaatt acgcattgag ttcggcaaat ggtaaaaaca
    55081 ccaacttcta tggcccgaca actccaaata atccgaaact cggtgacgtt tggtacaaag
    55141 aaaatggtga caagtttgaa atgtgggtgt atgaaatccg tgatggcgtc actcagtggt
    55201 atccacttat gacagatatg acggcagaag aagtaaagca agccgtggaa caagcacaaa
    55261 aggatgccaa tactgccact gaaaaggcca atcaagcatt taacgatgct gtgaatgctt
    55321 tgaatgcagc tgaaagtgta ggtgcaagtg ttgaaacgct tactacatcc gttgaacaga
    55381 atactagcaa gatttcatta gtggcgagtg gtatgtcaac tcttgaagga cgagtaatca
    55441 cagctgaatc aactctgaca gtgcaagctg ggcagattgc tagtaaggca agtcaatcga
    55501 cggttgataa tctgacaggg cgtattacaa ctgcagaaag caccatcacc caaaatgctg
    55561 atagatttga gctatctctt tctaaaacaa ataaagcggt caatgtttta gccggtatga
    55621 gcattgcaga tttgcagtgg gcagttggtg gtctgaatac aaatgatgga aaagaaaata
    55681 cctatggtgg atacgttcgc tcaggtttta caagagttag aaatggcgaa aagtatctac
    55741 ttcaaacatt tgtgggtggg aaactttact ctacttacgc aacggcttat ctgctttatt
    55801 acaaagagga taagtccttt ttatcgtaca cccaatttgg aaacaccacc acaccgctta
    55861 ctgttccaag cagtgccgct tttattcgca ttcgtttaac aactagtgat gcaccagaaa
    55921 ccatcagttg ctatatgttg aaaacggatg tgacaaatgg ctatgttgat ttatccgaga
    55981 taagtaacgt cgtaaaactt acagcgacca ctgatgcctt actgttaagt gtttcagaaa
    56041 ccaaagaagc aatcggcgtt ccatttaaag tcaatcaatg ggaacaagga agtttgagta
    56101 ccactgacgg ttcagaagtg tccgcaacca attatctccg aagtgagttc cttgatgtca
    56161 aagtgggaga taagtatatc gggcaactgc gtgacggaac tcaagctgct ttctactatc
    56221 attactacgg catggattta ccttacattg attttgtccc aacggcaaaa caatttagtg
    56281 aaaccctaac ggcgggaact tatactaatg cgaccattgc gagttatcta gtaagtaaga
    56341 acgttgaatc gatgagtatt acaagtggcg tgacaacaaa cccctattca aattcaggag
    56401 attatctggt catttacaaa ttgccattgg aaggcaaagt cgctccgagt gtaatttcgt
    56461 ggaatggtcg caaggataca tcagataata ttgtcttgct ttatacaggt ggtgcttggg
    56521 aacagatttc gaccgttaca agtagcagca atgccactta ttcttggacg ttaacggctg
    56581 agcagatatc agggattact actgacagcg tgtatctagc tttcttttca gttaagactg
    56641 gcacatatgc tgggatttat aattcaagca acacaccttt tactctaaac ccgtcggatg
    56701 gttcaagtta tcatcaaatc tcttatactg gttcgagcgg agcaattaca gtcccgagtg
    56761 gcacattgaa aatgcgagtc cgagtgaaca cgagcatcaa acccgatgaa tatgaaggaa
    56821 atatttatcc tacgaccaca aggcaagact ataccaatgc caacacatta tattcagcaa
    56881 ttctcatgca gaaagactta atcaatttac gagttggtaa aggagacgtg attaatcaaa
    56941 tcaatatctc acctgaaagt attttgattg ctggaaacaa ggtgcacatc actggtcaaa
    57001 caaccattga tagtgcagtg attagttctg ccatgatcca aaatgcggcc atcacaaacg
    57061 caaagattgc agatgccaca attcaaagtg caaaaattgc aagcattgat gcggcgaaga
    57121 ttaccacagg aactttgtcg gcagcccgta ttggcgcgaa ctcgattacg gctgataaga
    57181 ttgctgctaa tatcctaaca gcattgactg cgagttcgtc cattcgaatc acaggaacta
    57241 cgattggata ttattcagga agcactcttg tgactgaaat aaactcgcag ggaatgacca
    57301 ttcgacgtgg tggaacgaca gtcggaacaa ttggtgcaaa taacatcagt ggtcactctg
    57361 attggcgtgg attggtattt gatcttgagt atggaacaga gtatatgtca tgggctcata
    57421 aagattcctc ctctgcgagt gtctacaccc agaagctttc ttggtatgcg agaacattgc
    57481 agtctggtat ggagaagggg ttccactttg acgacgacat ttatttacca tcaatgcttc
    57541 gtatagggac acaacatcaa atatctgttg gtgggattac atttagtagt tccctttata
    57601 ctaagattgg ctcaagttcc ggcagcgctg gtattgcatt caacggttct aatctgatgc
    57661 tcggagatga tgggaagtgg gttgactttg gagtcatccg tgaaatctgt gccaaacttg
    57721 cgggacgaac aatcgcttta ccaaccggtt tcagtagcaa cggtacagcg agtggttggt
    57781 acaatccgca gtcgtttaat tcaatgacca catacagtta ggaggccgcc atgaacgaag
    57841 aaaaagaaga agtgatagta actgaaccta tcgaggcaga tattccaaag ccaatgaaag
    57901 ttgggacgga tatggttgat ttgcttgtga tggtcgtgga tgatctttgt gtccagattg
    57961 gtgatttaaa ggagaagata gataatgctc aaaattagaa atgaagattt ggtggacgtg
    58021 atgagtttct taaaaggttt tgacttacca ccaaagatta gccgagtgcg aacgaagctt
    58081 gcaaaactca tccaggtgaa ggttgatgaa ctctatcaag acgaggtaga cttgcttcaa
    58141 aaatacggaa agaaagatga gcacggcaat ttggtccaac atgacggtgc cttttcactc
    58201 atcccagaaa ccgcaactga atatcacaaa gaaaaggcag acttgctcaa cgaagaaacc
    58261 cacatcaacg tggatgaact tcaagacaag ctgcctttat tggttgctgg atttgaaaat
    58321 agtgaagtca ccatttcagg gaaagatgct gaaatgctcg atttaatttt agaacaattg
    58381 gaggatttgt aaatgaaaga agtgtggaat tggattcaac tcgccatcgc aggtggtgga
    58441 ggagtgattg gatggtatgt gggaggaatt gatggtttga tttatacgct tgtaactttt
    58501 gtggtagtgg actatgtaac tggtctgctt cgtgcaatca acgaaaagaa actatccagc
    58561 aagattgggg caaagggcat cactaagaaa atcatgattt ttcttttggt tggtgtggct
    58621 catatgcttg atgtggaagt catcaaaggt ggaaatgtac tgcgtgatgc ggtcattttc
    58681 ttctatatct caaacgaagg aatttcactg ctggagaatg ccgtggcgat tggactgcca
    58741 gtgccggaaa agttgcgtga aatcttgcag cagttacacg aaaagaagga ggaaacagaa
    58801 aatgggaatt agtaatctag caacaaaata tggatttcag tcatttagcc attactcgaa
    58861 tggtcgtagt ggtgccaaag tagataagat agtgattcat cacatggcaa ccaccaacta
    58921 tgatattgtt cctgatattt ggaaaacgcg tgaggcgtca gcacactacg gtattggaaa
    58981 aaatggagaa attcgagcat acgtggacga aggaaacaca gcttggcacg ctggtaattg
    59041 gaacgccaat atctcaagta tcgggattga gcactgcaat agcactggat caccgaactg
    59101 gggtatcaat caagcaacta ttgatgcaag tgcaaagttg tgtgcagata ttgccaaacg
    59161 ttatggtcta gggaaattgg tggtgaacaa gaatatcttc cctcactcac atttttcggc
    59221 cacatcatgt ccgggacagt tgcttggtaa actgcaggag attgcggata aagccaatgc
    59281 gattaataat ggttcatcaa ccccaagcac gccagctagt ggtctgtatc gtgttcgcaa
    59341 atcatgggct gacgccaagt cacaaattgg tgcctataag gacttgaaca atgcgaagcg
    59401 ggctgcagat agccattctg gttataaggt gttcgaccag aacggtaatc aagtttatcc
    59461 atctgggtca tcaacatcca acgtgaaaac tcttgtggtt caagtgagtg gtctcaacgt
    59521 tcgcaacaag ccaagtttaa gtggtgcggt tgttggttca tacaataaag gcgcaacttg
    59581 gacgcttccg aagtcagaga agatgacgat tgcggatggt tgggtatggg ctcgttgccc
    59641 gattggatat tgtgctgtcg gtaagaatac tggtaagacg gaatctgacg attatatttt
    59701 gatttcataa atttaatcgg aacaaaatta tgtccggatt gttgacttaa aatatcgaac
    59761 gtgttattat taattcaacc agaacataaa tttgttccga ttgaattata gaaagaggta
    59821 tcgatatggt tacgtttaaa gatttgttac cagaaccaac agaaagccaa caagctttca
    59881 ttttagatca cgggaaaaca gaagatgatg gtcagttgaa atacgctgat gatgctaaaa
    59941 gttacggttg gaacatgcgc caatatggta aattgaaagc cggcgcggtt gttttgaatc
    60001 gtcatccggg gaaaattact aaagaccgta agtgggaaat ttacgggggc ggatacgttg
    60061 agagcatttc tgaacctgat gaagctggaa atgttacggc agtaattaca cacgcattta
    60121 caatcgaacc ggcaattaaa caaggtgatt catttattga aaacttcgat tggaatacac
    60181 ctagcaaaaa gaagcgtaaa aagccgaatt catgggccta cttctgggat cagtatggaa
    60241 tgaatgaaat tacctatgcc gattttgttg cattgattga aaatcgtcat cttatcccaa
    60301 tcgatgatac acaatcgttg ccgtctgaac tggatcttac aaacgaggaa gttgaagaaa
    60361 ttgagcaaga aagttcaaaa ggatttactg ttcttgttga tgaagtggga ccaaatcgca
    60421 cgaatgggac tcagaaacga aaattcactg gacgccatac tgattgggag cgtgtcaaca
    60481 aggcaaaaca aaaaacgggt gctctaggcg aagaaatcgt acttgatttc ctcattcagg
    60541 aagcagaaaa gaacaaaaca aaacttcctg aacatgtttc taaaactgaa ggagatggtc
    60601 atggctatga tatttgtgcg tttgaccaat caggtaatga aatccatatt gaagtgaaag
    60661 cttcaaaaac aaacttctct gacggatttg aaatgagtac taatgaggtt gctgcctccc
    60721 ttggagatac accatataaa atttactttg ttcacgacct tgatgtggcg tcaaaagttt
    60781 gcaaaatcaa aatttatgat ggcccaatta ctgaagaaaa cttcacgatg gttccaacga
    60841 gctacaaaat tttcaataag tagtttgacg tctttataat tcaaaacagc ctcacaagtt
    60901 ttattgtggg gccttttttt cgtctagttt tctttaaggt aggtgtttac agcattttgc
    60961 attaggatta aatgatttct taactgaaat acctgctctt tgtaattgat gcagaaaaga
    61021 ggcgattcta ttgattccat acgattattc atcgcgttta tattggcttg taaaaactgg
    61081 tataaagctt cttcagcctc tatggtttta aagcactttt caaattcttg tttgaagctt
    61141 tcatcaaatt cctccagttc ctttgttgtc gcagatttgc aaaaatgttc atattctttt
    61201 tctaaccctt ccatatcaaa actactcata actttctcct tcccacaatt gatatttata
    61261 ttgttattta ttggatgaag cttgtaaagg cttatttgat gtttgggaag taaaaagtca
    61321 aaatttacaa agaataattc ctactatata taaacaaact tagaagcctg caagagtgat
    61381 tcgtttcatt cttgcaggct tttttcgttt tgtgggttcg agtcggttat ttctttcgca
    61441 tatggatgaa aggcaaagga aaatttgtga ttgggtttac tttcatggat tttcttcgcc
    61501 tgtatcttga ggaggcgaaa gcaatgacta acaagaaaac agaaattaac tattcagatg
    61561 aagcagccta ttacatttca cagcagatcc ttctgaactt acttgttacc ggtaaaatca
    61621 acctggaaga atacgtcaaa atcgacaata aaaaccggga aacattcaat ccttttttat
    61681 caaggataat ggtctgaaac gttgatataa cggcacttta gagctaacat gtcacctaat
    61741 ggaaaggagt tgagctgatg aaaacgatac ataaattggc acgtccagag ttgccggaag
    61801 cttccaaact aaaggtggca gcatacgctc gtgtatcaac atcaagtaat gaacagctgg
    61861 caagtttaca aacccagatt actcactatg aaaatcacat caagaaccat gaacaatggg
    61921 actatgtcgg tgtttattat gatgaaggga tttctggaac caagactgta aaacgtgatg
    61981 gtttaaaacg attgattaag gatgcagagc ttggaaagat tgatttgatt ctgacaaagt
    62041 ctatttccag attttcaaga aatacagttg actgcttgaa tctggttcgg cagctaactg
    62101 atattggtgt caccatcttt tttgaaaagg aaaacattaa tactggtgat atggaaagcg
    62161 agttgttgct ttcaatttta tcaagcttgg cagaaagtga atcctattcc cattcagaaa
    62221 acttgaagtg ggcgattcga aaccggatgt ctaaagggaa attcaaagct tacccgccgt
    62281 atggctacaa gcggaaaggc tatgattttc acatcgttgc agctgaagca aaagttgtta
    62341 aacaaatgtt ccagtgggca ttagatggca tttcgactgg aatgattgcc aagcggttaa
    62401 acgatggaaa acaattcact aaacgtgggg caaattggca agcaacatca gtcagaagta
    62461 ttttgaaaaa tgaagtttat accgggactt tgacgatgca acaaaccttt gttgatgacc
    62521 agtacaaacg acgcataaac aagggcgaat tgccaatgta ccagatcaaa aacaatcacg
    62581 aacccatagt atctaaagaa gattacgacc gagttcaaga actcattcgg agaaaagctg
    62641 aggccaaagg gaatactggt gacgggaaaa agtacaccaa gcgatacgca ttttcaggca
    62701 acatcatctg tgataaatgc ggcagcaaat tcaagcgggt tcacacttat tggaaaggta
    62761 atacgaagaa ggttcagtgg aagtgtacgg ggcatcttaa aaataaagat agctgcgatg
    62821 cattaccaat tacggatgaa gatttgaagg ttgcttattt gaccatgttg aataagttga
    62881 ttttcggaca caagttggtt ctggaacctt ttatgaagtc tttgaataaa gcaggtgcga
    62941 atcaggaagg gctcgaagtg cttgctgatg aaatcgacaa gattcaagaa aagcttgagg
    63001 ttctggcaag tttgactgct tccagtattt tatcaacgaa ggtgtcttta gaagaacaaa
    63061 gcaaacttca acaagagagg gctgtcgcac aagaaaagca gcgcctcatc attgagtcag
    63121 tcaacggcaa aagcactcag caaattgaac tagaagcctt atacaaattc gcgcggatgg
    63181 cagagatgtt gacggactgg gatgaagatt tattcaagaa gttcacaaac agcattacag
    63241 tttacaaccg ccaagaagtc ggatttgaac ttaagtgcgg actacttttg aaagagaggt
    63301 taccagaatg aacaatacaa ttccatacgg gtatgtcatt aagaatggag aaatcaacgt
    63361 ggatgaacag caagctcaac aaatacgtga gttcttcaat atttaccttg agggtgttgg
    63421 cttacaagaa gctggcaaac gctctggcat taacaagccg catcccttta tgaaacgtat
    63481 tttgatgaat gaggtttact taggaaatga gatatatcca aaaatcattg atgaggatat
    63541 tttcttccaa gcggatctgg agcaagaacg aagaattaag ttctttaaaa aacgcaagga
    63601 gatcaaagtg ccagatacga aggtaatacc cactcatttt aaatctggca agattaccga
    63661 aaagtattct gacccattca aacaagcgca atatgcatat tcaaaaatca aggaggttaa
    63721 gtgaaatggc aggaaaacag gtgacggtta ttccaatgaa gcctagacaa tgggcagcag
    63781 agaataccga agagaaacca aagttgaaag tggcagccta ttgtcgtgtt tctactgaaa
    63841 tggaggaaca ggcgagttct tacgaagcac aggttcaaca ctatacggac tacattcaaa
    63901 gaaatcccga ttgggagctt gtaggaatat ttgcggatga agggatttca ggaactggga
    63961 cgaaaaagcg tgacggtttt aaccggatga ttgaggcgtg tcaaaacggc gaagttgagt
    64021 acatcatcac aaaatcaatt tcacgctttg cccgtaatac ggttgattgc ttgcaataca
    64081 tacgacagtt aaaagaactg aagattgcag tgttttttga aaaggaaaac atcaatacaa
    64141 tggatgcgaa gggtgaggta cttttgacca ttatggcaag tctggcccag caagaaagcc
    64201 agtcactaag tcaaaacacc aagatgggcg tacagtatcg tttccagcag ggacagctac
    64261 gtatcaacca caatcatttt cttgggtaca ctaaggacga agatggtaac ttggtgattg
    64321 aaccaaagga agcagaagtc atcaaacgca tcttccgcga ataccttgag ggcagttcct
    64381 tgcaagaaat tgctaatggt ctaatggctg atggcatcct aacgggaggg aagcgaaagc
    64441 tctggcgagg agaaggagtc agattgattc ttcgaaacga gaagtacatg ggggatgcgc
    64501 tgcttcaaaa aacatatact accgatttct tgacaaagaa acgagtgaag aatgacggga
    64561 gttacgctca gcagtattat gttgaagata gccatccggc cattatccca aaagatatct
    64621 tcatgcaagc ccagcaagaa ctagaccggc gtaaatcaat gaaaaacaaa aagtcacagt
    64681 gcttttctgg gaagtatgcg ttatctggta tcacagtttg cggtgactgt ggcaatgctt
    64741 accgcagagt tcattggaag aatcgaggaa ctgtgtggcg atgcaaatcc agagtggata
    64801 aacgagaaca taattgcacg ggtcgaacaa tctacgaaac agacctgcat ggagctgtta
    64861 tcaaggcaat caatgaaacg ttaatcgatc gtgatgcttt tcttcagcaa ttaaatgaca
    64921 atatcaactc agttatgact gatggattaa ctgaccgatt ggcagaacta gacagtcagt
    64981 tgaaagagct tgaagctgaa attattagcc tggcagttgg cggtcagggt tacgatgaat
    65041 tggcaagcca aatattttca ctaagagatg aacgagatgc ggttgcaaaa caaatagcag
    65101 cgaatactaa tttgcaacaa cgggttgatg agatggtagt ctttgtaaaa gaacacgatg
    65161 tcatcaatga gtataatgaa gtgcttgttc gaaggttgat tgagaaggtc accatttttg
    65221 aaaagaacat tgttgttgat ttcaaaagtg gggtgagagt gactgtagaa atttaatttg
    65281 aatagatagg actagacgtt aatgtgggta gccatattaa cgtctttatt gattggattt
    65341 cataaagtga ttgatgatat aatatattat gtaaaaaata ggctgggggc gaagttctgt
    65401 gagccaatgg aataccattg atgaattaag ggatattttt attcgtaaat atacggattc
    65461 tgagttgagt aatgaacttg aaaaggcctg cagcgaagaa tatgaattga tgcgtgatta
    65521 taatgggcgt cagattttag agttattaca gaatgtagac gatgcctatg gagatataaa
    65581 atcagaaaat gatgctagca atgacgaagt gaaagttcag ataacatata aaaataatat
    65641 cttggaagtt gggaatacag gcacttcatt ttcaaaagat acaattgaaa gattgtgctt
    65701 aggaagagcg agtaataaat catcacaaaa tatcggaaat aaggggactg gttttcgttc
    65761 cttgctaaac gatgctgagt gggtcgaact gtattcggga gaatttgcta ttcgatttag
    65821 tgaagaattc acaaaaaagc tttttgagca gtatgtatca agagattctg acaaattcag
    65881 tgaattaatt tttgagcaga agaaaaattg gaaaaaagaa gattatgatt tatgttttcc
    65941 aataatgaac tgtcctcaac caataaacaa aattgattct ggttttgata cattaattcg
    66001 agtaaaacta aaagaaacta atttaagtaa aggcacgagt gtttctaaac aacttcagca
    66061 gccattttat aagtcactgt tatttttacc aaatataaca aaaattgtta ttgaaacaac
    66121 tgatgatgaa aaaatatcag agaaaattgt ggatggaaat gatgtattga tagaaaaaag
    66181 tgatgttact ggtcaatcac cgtctgaaga atattttgtt tttgacaaag aatctatgat
    66241 aggagataaa gttgctaaaa ttgcactagc cgtttctaaa gattctgatt acgattactc
    66301 caaagaaaaa ctatattgct actttccagt acgtaatttt ggtacaccaa ttcatgcact
    66361 tataaatgca ccgtttataa caaataattc acgtgatgac gtcccggatg atagtgaaca
    66421 aataaataag aaaatattta ttgaggtgct caaatttatt tctgaagtag cagaaaaatt
    66481 atctaagcct gaatatgaag acatagcatt aaaaatggta actcctttta aagagaataa
    66541 actatgggac ttagatgtat ttgacttaat gaacgaattc ttgggtattt tatctaatgc
    66601 ccaaatacta ccgactgtca attggcaata tatttctatc aaagataatc caaaactttt
    66661 ccttcaagaa ttccctgagg agctaatagg tgagacattt tccaccttgt tgcaattatt
    66721 agaaacaaac gaaaatgacc aactagtaca agaacttgct aattataatg aatacactgg
    66781 cctgtcgttt gttgtagatg aactatctga taaaatcaac attctttcat cttcttggga
    66841 aaatagaata aggacaaaag tgttcttatg gtggagtgag caatataaaa gttctccgat
    66901 tgtccctaag ctattaaagg acaatagtga caattggttg atgaaatcag ataaggtatt
    66961 cttaccgacc gatacaggtg ttactgtgct tccaaaagag ctgtcttctt gggttagttt
    67021 aagcattttg aaacaaacat atgttgatga gttaatttat cagattaaga cttcatcaag
    67081 ttggaaaaat aaatgggaag aaacagctaa tgcatacaaa gctgaacgta caggagataa
    67141 acgtattcta gatgcattca gtgaaaaata tcttgcaatt gaattcactg agcaaagcag
    67201 ttcagatttg atcattggaa cgattaatcg acaaattgac actgttgaaa aatctatcag
    67261 tttcattaat tggttttatg ataactacaa tgaaaaacta tcagctggaa gtgagctgtc
    67321 taaagttcca tttaatcttc caaacgttgc tggtgacgtt caaccttcca ataaaatgta
    67381 tattggaaaa gagtatggca atgaattagg agataagctt tttcaagata cagattacac
    67441 ggcattgaaa aaaatagatt gctctagttt tgctgaggag agagattttc tgtcatttat
    67501 tcttcgatgt ggtgtttcca aatatcctaa gatttatgat aacgattctt taaaaaatga
    67561 tgttggattt aaaaagttta tttataacaa atataagaat gaaataaaat ttacgaatat
    67621 taattattta acttctaagt acatcagtaa ttttgaagaa ttaatagaca aattagatac
    67681 gaaagacatt gtcaattggc tcaatcaaga cgaagaattg acagatttat tatcttcggt
    67741 aaaaaaagaa tcaagtgcaa gtcagcaatc gaactggagt ggattgtact ttaattcaaa
    67801 tgagtatgtg aaatacattt tgaataaaac tgcatggatt aagattgatg gagaaaaata
    67861 tagcccgaga caaattgtgc tatatgaaaa gctccaggca cacgttccgg gattgtatgg
    67921 agtttctgaa caaagcttaa tggaactctt ggggcaacgc attgttgaac agttggattt
    67981 cagaaaaaat atggcgatgt tttcggatga tgaaataaga gctatattag ttgagttacc
    68041 aaaattcgat aaaggcgaaa taagtcgcag attatataat gaacttataa gaaataaaaa
    68101 aggaatgaat cctacttatt ctaccgaagg attatcagtc ttagccaaag atggcttctt
    68161 ttatccaaac aaagaattaa ggtatgctga taagcgactg cctaaatcaa tagaaaaaca
    68221 aatgcgtttt atttacgtgc ctgaaaaagc tagtacaact acgatatatg aatggcttgg
    68281 ggttgaacgc ttcaaaacaa atttaaaatt ggaaacattt gagttattgg aagattattc
    68341 tgaggatttc aaaaaagaga ttgatgatat caaaactgcg gtattatcta cgatagatga
    68401 caatagtaag aatgttagtt cattaaaacg tattcagatt attccatgca gcaaaatatc
    68461 tgctaaagat gttgagcaag atggtcagtc aatcgttttg gaagattatt attttgtgga
    68521 aagtaaagga aaattctact taaaattacc tgctaattct attgaaatta atcaattacg
    68581 tttgattgat acgttttctt caacaatcgt agacatattc aagcaggtgt taaatctaag
    68641 cttagatttg aatttgattg aattgttaat ttcccgagat acggaacata aacaaagaaa
    68701 aataactgaa gaattcggtg tagataagtg gaatgaatcg tttgagctat tgtttagcga
    68761 aaatgcgtta aataaaaaag ttaatgactt tttgttatca aatggactaa atgaagagga
    68821 atatcctacc atttttgaaa ttgactattc atatgcatta aaagaagaag agtatcaatt
    68881 gttggcgata gctctagaaa gcatttcaaa agatattgag gatttgaatg gattgtcaga
    68941 attgattcat ttggatattc gtgaattttg gaaactgaaa ttaagatctt atctagataa
    69001 gaaagcggaa cattaccgcc agattttata tagcaatgca aataaaagcg gtgatgaaat
    69061 actcaaagaa acattcctag aaaaaattga tagatacaaa gattatcaat ttgagatttc
    69121 tgaaattcca aattcagttc aagtcgacat tgagaaagaa agtaagaaga gatttccaga
    69181 gctaagtgtg tcaattactg atgaaattga cattgatggc ctgtatgata ataatgtcga
    69241 agagttagtc acggttgcag ctattacaat agatgatttt gattattata ttagaggtca
    69301 tcaaaaagtt tcttccttac tgtattttgg tattccgtcc cgtttaagag aagatatact
    69361 gatattcgtt actaaagaaa atgatgttga tttgaacgga gaaaaatctg gtatctctat
    69421 tgaaggaaca tcaactgttc aaaccattct tgaaccatcg aatagtgtta gtaggaaagg
    69481 taagaacaca cgaaatggag aaagaagtaa acgagattat gataatagaa actccgcaaa
    69541 tgatgatgct ggtgaaacag cagaacaaat tgcttacgct gaattgactc agaatcaaaa
    69601 acttaatgta atttggaata cgaaatattc tagtaaatct tcagatcgta ataagcagcc
    69661 accaaacgga attgtttgtg atatgtgggt tcatgaccca gagaatggga atatgtactt
    69721 tgaggtaaaa tcatcaatta cagaatttga aatgtcgatt aatgaatata attctatgtt
    69781 agataaccct gactcttacg aggtcgtttt agtcaatcgt gataccaaag agataagtcg
    69841 tcataaattt gatgaacttg atggattgaa acaggcaagt agctatcttt tcaaatttaa
    69901 gcaaaagaga agtgacggtt gagtgatttg tttccacaaa cctattcaat ctatcaactc
    69961 aaaaatcata aacaaccatt attatagtag gaagatacag agtgtactct caacgcatat
    70021 cgaattagtc gcttcattcg ttaaaggaga ataaaaatgg accaaaaaga aaaaatgaaa
    70081 gaattgatca aaaagaaaca aggtggaggc cgatccaaac aaatggaaac acctaagaat
    70141 gatcgtaaaa acatgagaaa aggccctaaa atctttaata aatagacgtg ctaaagaaag
    70201 actaagaagt gttaactttt gttattgttt cttagtcttt tta
//
LOCUS       DAYYGY010000005        66154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000005 DAYYGY010000000
VERSION     DAYYGY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.005"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(165..464)
                     /locus_tag="ACHA52_000325"
     CDS             complement(165..464)
                     /locus_tag="ACHA52_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8939082.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            815..1447
                     /locus_tag="ACHA52_000326"
     CDS             815..1447
                     /locus_tag="ACHA52_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8939083.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1570..2208
                     /locus_tag="ACHA52_000327"
     CDS             1570..2208
                     /locus_tag="ACHA52_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8939084.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2327..2399)
                     /locus_tag="ACHA52_000328"
     tRNA            complement(2327..2399)
                     /locus_tag="ACHA52_000328"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2364..2366),aa:Thr,seq:cgt)
     gene            2520..2762
                     /locus_tag="ACHA52_000329"
     CDS             2520..2762
                     /locus_tag="ACHA52_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939085.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2901..3530)
                     /gene="upp"
                     /locus_tag="ACHA52_000330"
     CDS             complement(2901..3530)
                     /gene="upp"
                     /locus_tag="ACHA52_000330"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8939086.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3664..4611)
                     /locus_tag="ACHA52_000331"
     CDS             complement(3664..4611)
                     /locus_tag="ACHA52_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8939087.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4764..6008)
                     /gene="glyA"
                     /locus_tag="ACHA52_000332"
     CDS             complement(4764..6008)
                     /gene="glyA"
                     /locus_tag="ACHA52_000332"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8939088.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6183..7208)
                     /locus_tag="ACHA52_000333"
     CDS             complement(6183..7208)
                     /locus_tag="ACHA52_000333"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8939089.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7401..8240)
                     /gene="prmC"
                     /locus_tag="ACHA52_000334"
     CDS             complement(7401..8240)
                     /gene="prmC"
                     /locus_tag="ACHA52_000334"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8939090.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8233..9306)
                     /gene="prfA"
                     /locus_tag="ACHA52_000335"
     CDS             complement(8233..9306)
                     /gene="prfA"
                     /locus_tag="ACHA52_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8939091.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9309..9893)
                     /locus_tag="ACHA52_000336"
     CDS             complement(9309..9893)
                     /locus_tag="ACHA52_000336"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8939092.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9999..10247)
                     /locus_tag="ACHA52_000337"
     CDS             complement(9999..10247)
                     /locus_tag="ACHA52_000337"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8939093.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(10238..10468)
                     /locus_tag="ACHA52_000338"
     CDS             complement(10238..10468)
                     /locus_tag="ACHA52_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939094.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            10574..11176
                     /locus_tag="ACHA52_000339"
     CDS             10574..11176
                     /locus_tag="ACHA52_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8939095.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11280..11849)
                     /gene="lepB"
                     /locus_tag="ACHA52_000340"
     CDS             complement(11280..11849)
                     /gene="lepB"
                     /locus_tag="ACHA52_000340"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8939096.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(12079..12846)
                     /locus_tag="ACHA52_000341"
     CDS             complement(12079..12846)
                     /locus_tag="ACHA52_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8939097.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12843..13322)
                     /locus_tag="ACHA52_000342"
     CDS             complement(12843..13322)
                     /locus_tag="ACHA52_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8939098.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13447..14184)
                     /locus_tag="ACHA52_000343"
     CDS             complement(13447..14184)
                     /locus_tag="ACHA52_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8939099.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14345..15745
                     /locus_tag="ACHA52_000344"
     CDS             14345..15745
                     /locus_tag="ACHA52_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8939100.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15968..17320
                     /locus_tag="ACHA52_000345"
     CDS             15968..17320
                     /locus_tag="ACHA52_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8939101.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17313..18011
                     /locus_tag="ACHA52_000346"
     CDS             17313..18011
                     /locus_tag="ACHA52_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8939102.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(18073..19224)
                     /locus_tag="ACHA52_000347"
     CDS             complement(18073..19224)
                     /locus_tag="ACHA52_000347"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8939103.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19237..19674)
                     /locus_tag="ACHA52_000348"
     CDS             complement(19237..19674)
                     /locus_tag="ACHA52_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8939104.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19689..21455)
                     /locus_tag="ACHA52_000349"
     CDS             complement(19689..21455)
                     /locus_tag="ACHA52_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8939105.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21719..22396
                     /locus_tag="ACHA52_000350"
     CDS             21719..22396
                     /locus_tag="ACHA52_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8939106.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22399..23526
                     /locus_tag="ACHA52_000351"
     CDS             22399..23526
                     /locus_tag="ACHA52_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939107.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23726..24166)
                     /locus_tag="ACHA52_000352"
     CDS             complement(23726..24166)
                     /locus_tag="ACHA52_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8939108.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24177..26210)
                     /locus_tag="ACHA52_000353"
     CDS             complement(24177..26210)
                     /locus_tag="ACHA52_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8939109.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26212..27915)
                     /locus_tag="ACHA52_000354"
     CDS             complement(26212..27915)
                     /locus_tag="ACHA52_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8939110.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(28009..28959)
                     /gene="manA"
                     /locus_tag="ACHA52_000355"
     CDS             complement(28009..28959)
                     /gene="manA"
                     /locus_tag="ACHA52_000355"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8939111.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28998..30023)
                     /locus_tag="ACHA52_000356"
     CDS             complement(28998..30023)
                     /locus_tag="ACHA52_000356"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8939112.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHGG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30232..31101)
                     /locus_tag="ACHA52_000357"
     CDS             complement(30232..31101)
                     /locus_tag="ACHA52_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8939113.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31805..33697)
                     /locus_tag="ACHA52_000358"
     CDS             complement(31805..33697)
                     /locus_tag="ACHA52_000358"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939114.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33694..35421)
                     /locus_tag="ACHA52_000359"
     CDS             complement(33694..35421)
                     /locus_tag="ACHA52_000359"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939115.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35437..36087)
                     /locus_tag="ACHA52_000360"
     CDS             complement(35437..36087)
                     /locus_tag="ACHA52_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8939116.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36291..36587
                     /locus_tag="ACHA52_000361"
     CDS             36291..36587
                     /locus_tag="ACHA52_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8939117.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36779..37399
                     /locus_tag="ACHA52_000362"
     CDS             36779..37399
                     /locus_tag="ACHA52_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8939118.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37491..38657)
                     /locus_tag="ACHA52_000363"
     CDS             complement(37491..38657)
                     /locus_tag="ACHA52_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8939119.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38669..39022)
                     /locus_tag="ACHA52_000364"
     CDS             complement(38669..39022)
                     /locus_tag="ACHA52_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8939120.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            39150..40172
                     /locus_tag="ACHA52_000365"
     CDS             39150..40172
                     /locus_tag="ACHA52_000365"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8939121.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            40191..40352
                     /locus_tag="ACHA52_000366"
     CDS             40191..40352
                     /locus_tag="ACHA52_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939122.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40611..41045)
                     /locus_tag="ACHA52_000367"
     CDS             complement(40611..41045)
                     /locus_tag="ACHA52_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939123.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(41155..43014)
                     /locus_tag="ACHA52_000368"
     CDS             complement(41155..43014)
                     /locus_tag="ACHA52_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8939124.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            43015..43155
                     /locus_tag="ACHA52_000369"
     CDS             43015..43155
                     /locus_tag="ACHA52_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8939125.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(43132..44292)
                     /locus_tag="ACHA52_000370"
     CDS             complement(43132..44292)
                     /locus_tag="ACHA52_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8939126.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44599..46023)
                     /gene="araA"
                     /locus_tag="ACHA52_000371"
     CDS             complement(44599..46023)
                     /gene="araA"
                     /locus_tag="ACHA52_000371"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8939127.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(46039..46737)
                     /locus_tag="ACHA52_000372"
     CDS             complement(46039..46737)
                     /locus_tag="ACHA52_000372"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8939128.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46694..48355)
                     /locus_tag="ACHA52_000373"
     CDS             complement(46694..48355)
                     /locus_tag="ACHA52_000373"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8939129.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48573..49973
                     /locus_tag="ACHA52_000374"
     CDS             48573..49973
                     /locus_tag="ACHA52_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8939130.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50225..51304)
                     /locus_tag="ACHA52_000375"
     CDS             complement(50225..51304)
                     /locus_tag="ACHA52_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8939131.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51390..52349)
                     /locus_tag="ACHA52_000376"
     CDS             complement(51390..52349)
                     /locus_tag="ACHA52_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8939132.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52361..53872)
                     /locus_tag="ACHA52_000377"
     CDS             complement(52361..53872)
                     /locus_tag="ACHA52_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8939133.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53894..54457)
                     /locus_tag="ACHA52_000378"
     CDS             complement(53894..54457)
                     /locus_tag="ACHA52_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8939134.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(54463..55326)
                     /locus_tag="ACHA52_000379"
     CDS             complement(54463..55326)
                     /locus_tag="ACHA52_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8939135.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55340..56236)
                     /locus_tag="ACHA52_000380"
     CDS             complement(55340..56236)
                     /locus_tag="ACHA52_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8939136.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56411..57700)
                     /locus_tag="ACHA52_000381"
     CDS             complement(56411..57700)
                     /locus_tag="ACHA52_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8939137.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57672..59189)
                     /locus_tag="ACHA52_000382"
     CDS             complement(57672..59189)
                     /locus_tag="ACHA52_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8939138.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59387..60277
                     /locus_tag="ACHA52_000383"
     CDS             59387..60277
                     /locus_tag="ACHA52_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8939139.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60350..61174)
                     /gene="nadE"
                     /locus_tag="ACHA52_000384"
     CDS             complement(60350..61174)
                     /gene="nadE"
                     /locus_tag="ACHA52_000384"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8939140.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61186..62658)
                     /locus_tag="ACHA52_000385"
     CDS             complement(61186..62658)
                     /locus_tag="ACHA52_000385"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8939141.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63295..63657)
                     /locus_tag="ACHA52_000386"
     CDS             complement(63295..63657)
                     /locus_tag="ACHA52_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8939142.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63880..64632
                     /locus_tag="ACHA52_000387"
     CDS             63880..64632
                     /locus_tag="ACHA52_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8939143.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64772..65695)
                     /locus_tag="ACHA52_000388"
     CDS             complement(64772..65695)
                     /locus_tag="ACHA52_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8939144.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65710..65982)
                     /locus_tag="ACHA52_000389"
     CDS             complement(65710..65982)
                     /locus_tag="ACHA52_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939145.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19149 a        13544 c        11483 g        21978 t
ORIGIN      
        1 atactagctg tttttgtgtt aattaaagga taaaatacaa tataaagaaa catctgtttc
       61 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
      121 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      181 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      241 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      301 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      361 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      421 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      481 aaatatattt cttcccacta cattctataa acaaagtata cgctacatct agattttttt
      541 aaatcattac tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt
      601 aaaaacattc taaattaaat cagtaaaaat ttttattcta agatttttat tagaattacg
      661 cctaatttgt gtctctcagg gcaattctaa gaacgtttca agagatagcg acactatcgt
      721 tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg taagttggtt tagcttttgt
      781 taatatgact ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc
      841 aagcttgaat aaaggatcct tttttcatat ctatgaaaac gaagattttg aactgtataa
      901 tatcctttca caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa
      961 atctattttc aagtatgcat tttttgcttc caatgatttt caaagaatgg agattaccga
     1021 ctgtatattc gaaaactgtg atttttcagg ctgtatattg acaaaaagca tgttgcatcg
     1081 cgtgaaattt gtcaattgta aattaacagg tactaaattg atggaaagtt atattgggaa
     1141 cacgttattc gagaattgta aaatggacta tgtaaattta tctggttcaa acataaaaga
     1201 aagtaccttt gagcactctg tcttatcaag cgcagatttt gtagactgtt ccctcactaa
     1261 aataatgttt tctactaatg atttagaaaa tgctagcttt tttgatactg atttaaaaaa
     1321 tatcgattta agtagcaact cctttgaaat gattgaagta cgctcggaac atctaaaaaa
     1381 ttgtacgctt aatcagttgc aagcacttgg ttttgctaga aaatttttac aaataaaagt
     1441 agtataatca aaatgaatat ataagataaa tttttagcaa tacaaatatc cataaaaaaa
     1501 cattgcctat tttaactctt catgtatact gactctatct tcaaaaactg aatagtagga
     1561 gttattatca tgaataacat tttaggaaaa aaaatagaac ttaaaaaagg gatattgggc
     1621 actattgtcg ttgtactcgg tttgttaggg ttgctatttg cgccatccat tattagcatt
     1681 ttttctttaa ctatcactgg atttatagca tttacacttg tttttggtaa aaaagatacg
     1741 aaaaaaatgt tctcgaaacc tgtcgctcct gtcaaaaata tagccaaata tttttttatc
     1801 aatgtagtaa tcagtgccgc agtctcggta tttttgcaac aaattttgaa atggggttta
     1861 acaggcaatc caataaatga ggctttcagc ccgttattat ttattatttt gcctatcatg
     1921 atacttggag aagaattgtt ctcagtgtat tttctcgcta ttttttctag taagttcagc
     1981 attcctgtcg ctagtttctt gtcagcaatc atctttggat ttatccatta cagtacttat
     2041 gataatggaa atattttaca cactgtagca cacatccttt tgatacaagg ggtggcaaga
     2101 ttgttattca accaagctgc cattaaaagc aactcgatca ttacaagttg ggcagttcat
     2161 gtgatatttg atttcacagc tatattgcta gtcgttttat tctcctaaat ttttatagaa
     2221 ataatacatg ctctattgct aagaaagcat actttatttc ctttaaacaa aaagacagat
     2281 gtatcaatgg attaaaaatc cttgatatat ctgtcttctc ttttaatgcc agctataggg
     2341 atcgaaccta cgacctacgc gttacgagtg cgttgctcta ccaactgagc taagctggcc
     2401 ttatccaaaa gaaattatat actctatttg gctgattgta aagagtccga tggaaaatta
     2461 gtttaatttc atgatatttt tcataaaacg tggtataatt gatgtgaaag gcaggtttta
     2521 tgaaaatggc aacttttgga aaatttgata caactatcgt accatcggaa attggtgaaa
     2581 aattcaagat caatactcac gtaatgttca caattcacgg caagtcgcaa actggaacga
     2641 tttcaaagca attgaaaaat tccgctgttg ttgagattga tgaaactgca gaaaatgcga
     2701 acttgatgac gcaaagtaag ggtgtagtaa tcgttaacta caaagaacta aaaaagattt
     2761 gaatttgacc atctctgcat ttttgggatg gtttttttat atgcaaacga aagctgtgaa
     2821 aaagctgttc tgcatcaaac gttaaaaatg accaaaaaag gatgtgacaa aagttgtgtc
     2881 acaccctttt tcgtatttac ttattttgta ccaaacaaac ggtcacccgc atcccctagt
     2941 ccaggaacga tataaccatg ttcattcaat ttttcatcta gagaagctgt atagatgtca
     3001 atatcaggat gcgcttcttg caaagctttt actccctcag gtgctgctac caaacaaaca
     3061 aatttaatgt tcgacgcgcc acgttctttc aatgcatcga ttgccatgat tgctgatccg
     3121 cctgttgcca acattggatc tactacaaac aactgacgtg catcaatgtc ttctggaagt
     3181 ttgacgaaat attcatgtgg ttccaatgtt tcttcatctc gataaagacc gatatgtccc
     3241 actttagcag ctggaatcaa ttccaagata ccgtcaacca ttccaatacc tgcgcgcaag
     3301 attggaataa tcgctacttt tttaccagac aacgtttttt gtgttgtttc agtgatcggt
     3361 gtttcgatca cgatgtcttc caatggcata tcccgtgata cttcatatgc catcaacata
     3421 gcgatttcat cgactacttc acgaaaaacc tttgttccgc agtttttgtc ccgaataata
     3481 gttagtttgt gttgaatcaa tggatgatcg atcacttgaa atttgcccat tttttaagct
     3541 ctcctttgtt ctgtcgtttc tttttaattg taaaacaaaa tcaagtgatt gactagtcaa
     3601 actcgccatt tttcctaaga aaatccgttt ttgaaaagaa gttttcctgc ggacaagcaa
     3661 agcttattct tttttcttga tccaagaagt agataatgcc attgctgcac gcgttgcctt
     3721 tgtctgatcc atagcattta ccatgacaaa atcatggatg attccttgga agcgtacttg
     3781 tgtaacagga acccctgcat cacgaagttt gcgcgcataa gcctcacctt catctcttaa
     3841 tacatccgct tctcccgtta aaatcattgc tgaaggaaga tcatttagtt catttatatt
     3901 tgctctcaaa ggcgaagctg tgatttcagc acgctcttct tcattcgtcg tatattggtc
     3961 ccaaaaccac atcattcctt ctcttgtcaa aaaatagttt tctccgaatt catgatagga
     4021 atctgtatca aaatcagcgt ttgtgactgg ataatacagc aattgcttgg atattgggag
     4081 gccctttcgt tcttttgtta agatcgtcat gactgtagcc atgttaccac ctactgaatc
     4141 tcctgcaacg attagattct cgagatccag attttttgac ttggctaatt tattcagctg
     4201 ttgcaacaca gcataatttt gttcaattgc tgttggatat tttgcttctg gtgatcgact
     4261 atattctgga aagacaacga cggaattcgt acgaaccgca agttctctga tcaatttatc
     4321 atgggtttga gcactgccaa aaacccagcc tgctccatga atataaaaaa taactggtaa
     4381 tttatccata ttcccttctg gtcgtacgag acgaatattg agttttcccc attcgccagt
     4441 atccaccatc aagtcttcaa tatcgacgga atatttgttc acaggtgatt cttgtacttc
     4501 ttctagtaaa actcttcctt cttccggtgc tagttcatag atacgcggat gaggcgcatt
     4561 tgcatcactg aatgctactg cttccttttc taacgagata cgatcagtca tgacttttcc
     4621 tccctgagat ttagtgtgaa gactaaaatt acacctcata tactagagag catcaccgat
     4681 attgtcaaag aatccgtttt cccaaaaaat aaacagaaaa gcccaacatt gaacttttct
     4741 gtttactaat ggaatttaaa tttttatttt ttatgcaacg gaaatttttc agttaattcg
     4801 cgtacacctg ttttgacttc gtctagttga gcgttatcat cttttgcttg taatgctttg
     4861 acaactagtt cagccacttt tacagcatct tcttctttga acccgcgagt agtgattgct
     4921 ggcgtgccga ttcgaatacc gcttgttttg aatggactta gtgtttcaaa aggaatcgag
     4981 tttttattta cagtgatatt cacactgtcc aaaatgctct ctgcttcttt tccatttata
     5041 cctaactcgc gaacatcgat caacatcaga tgattatctg tcgcccctga aatgactctg
     5101 gttccaattg cttgattgaa aactttgacc attgcttttg cattggcgat gatttgttca
     5161 ctatattctt taaatgcagg atctaatgct tctttgaatg ctactgcttt acccgcaatc
     5221 acatgctcta gagggccgcc ttgaatacca ggaaataccg cactatttat tttttttgct
     5281 aatgcttcat cgtttgtcaa aatcatgccg cctcgagggc cacgcaatgt tttatgagtg
     5341 gtagtcgttg tgatatctgc ataaggaact ggattaggat gaagtcctgc tgctactaat
     5401 cctgcgatat gtgccatatc taccatcagt tttgcgccta cttcatctgc gatttctctg
     5461 aatttcgcaa agtcgatcgt tcgtccgtaa gcactggcac ctgctacgat caattttggt
     5521 tgatgttttc tagctaaaat acgtaccaca ttataatcaa taacttctgt tgtcggatca
     5581 acaccgtaag caacaaagtg ataggttttg ccactgaagt tgactggtga tccatgtgtt
     5641 agatgtccgc ctgcggaaag atccattcct aagatcgtgt cccctggttc taccaaagcg
     5701 agataagcag ccgtgttggc ttgagagccg gagtgaggct ggacgttcgc aaatttcgct
     5761 ccgaataatt cttttgcacg gtcaatcgct aaattttcaa cgatatcgac aaattcacac
     5821 ccaccataat agcgatgccc tggataacct tctgcgtatt tatttgtcaa gatacttcct
     5881 tgtgcagcca tcactgcttc ggagacaaag ttttcagagg caatcagctc caaattatgt
     5941 tcttgtcttt cttcttcttt ggcgatcgca gcccaaagat cagggtcaaa cgttttgtaa
     6001 tctaccacaa acaacagctc ctttttattt tcctacaatt ttatttttcc catcaaaaac
     6061 aaattgccaa atattcgtat ctcttctatt acgattagtt acgcttagtt tttcgttcac
     6121 tgatagaaga tttacttgat agcattgtat catactaaaa gaatgcgaca ataaccaatt
     6181 gatcattttt caaaataatt ttgtcctgct gcttttttca agcgattcat gtaagcattc
     6241 cccaatccag tttctggata cacttgtaca aaaatcaaat ctaatccaag tgttggttca
     6301 tctaatcctc ttagacctgc aaacaatcct ttagccgccg cttctactga gttgtcgtta
     6361 tacatataaa cagctgcagt atctgtccgc acttgatccg caatttcggg gcttgctatg
     6421 acacctgcac ggatcttctt attttttgcc cattgaactg cagttgacca atcgccatct
     6481 ctgaccatca gaacacgtgt atcgggtgag tagtgtttgt atttcatccc aggggctttt
     6541 ggtgtttcat tttcttttac aagatgctga tcaatagcaa caggagattc gatgactgct
     6601 tcgatttgtt ctttcgttac tgcacctggt cgtaaaatca taggcatggc tgttggatca
     6661 cttaaatcaa gaacagtgga ttctacacca attcgcgtag ctccatcatc aataatcccg
     6721 gtaatttttc cttgtagatc atgatataca tgatctgcag tcgttggact tggttttccc
     6781 gacgtatttg cacttggacc gacaagcgga acaccggaaa gttcgattac ttctagtgtt
     6841 tttttgttat ccggtatacg aaatgcagct gtggataatc ctcccgtcac aactgagggc
     6901 aaagtgtctt ttttgatctt aaatatcaaa gttaatggtc caggccagaa gttcttcaca
     6961 atcttctctg ctgccggatg aaagttgtcc acatactctt ttacttgttc aaaagaggcg
     7021 acatggacga ttaatggatt atcttgcggt ctccctttta cagaaaacac tttcttgaca
     7081 gcgtttggta aaagagcgtt ggcgcccaat ccgtatactg tttcggtagg aaaagcaaca
     7141 agttctcctt ctttcagtgc ttcggctgcc tcttgcaatt ctgtttcatc atatcgtttc
     7201 gtttccacag tttatccaca accccttttt catacattct tattttgtct gagtttatcc
     7261 tcaagttttc cactgtttgt ggataatcta tttgtcaata gtggataaac ttgttgataa
     7321 accaaaaagt cgtttacatc cttgtcaaat aagtgttttt tataataaaa aaacaaataa
     7381 aaaatttttc atccacaact tcaaccataa acttggatca ttcgttcatt gccaaaaaga
     7441 tcctttttta tcgccacttt tttatctgga aatgcttgct gaaatatttt ttttactgct
     7501 tcaccttggc gaaatccgat ttctaaaaaa atctttccat ttgattttag tacgcttggt
     7561 gcttcaactg ctattttttc ataaatagct aatccatcgt tctcagcaaa aagggccatt
     7621 ttaggttcga atgaccggac actttcatcc atcagttccc attcattccg actgatgtac
     7681 ggcggattag aaataatcac atcaaattgc tgatccatta ctggtcctaa tgtatctcca
     7741 tgataaaaac tgattttcgt tccttctttc tgagcatttt ctttcgctac ttctaatgct
     7801 tcttctgaaa gatccacagc agatatatgc caattttttc ttgctgcttt caaactaata
     7861 gcaattgctc cagtccctgt cccaatatct atcacttcta acggtacacc aggcgtctcg
     7921 tctaaacacc attcgactaa ctcctctgtt tctggtcgag ggatcagtgt tgcttcagtt
     7981 acctttaacc gatgatcata aaaatctgca tatcctagca aatactgagg tggatgatct
     8041 gcaagcagaa gtcgcagatc ggtttcgatc aaccgctgtt cttcttctga aatctcttcg
     8101 ttcatatgaa ggagccaatc aagttttttc cattgttttc gttccaaaaa taaaaattgg
     8161 atactatatg cttctttccc atgatcctct aaaaaagaag aagcccgtga caggacttct
     8221 cgataggtca ttttacccat tttgcatagc ttccagtttt gatgtttgat catacatgat
     8281 caatgcatca acgatctcat ctaatttccc tgctaaaatc tgatctaatt tttggatagt
     8341 taatccgata cgatgatcag tcacacgatt ttgcgggaaa ttatacgtcc ggatccgttc
     8401 tgaacgatcc ccagttccga ctgctgattt tcgattggca tcatactcgc tttgtgcttc
     8461 ttgctggatc ttatcgtaca cacgagcacg caagattttc attgcttttt cacggttctt
     8521 gatttgagaa cgttcatctt gcatagcaac aacgatccct gttggtaagt gagtcaaacg
     8581 tacagcagat gctgttttat tgacgtgctg tccgccagca cctgaagcat gatagatatc
     8641 tgtacggata tctttgtctt caatatcgat ctctacttct tcagcttctg gcatcacgac
     8701 aactgttgcg gtggaagtat ggatacgccc ttgcgattca gttgaaggaa cacgctggac
     8761 gcggtgcgca ccactttcat attttaattt cgaatatacg ttgtctccgg tgatcatcat
     8821 gatcacttct ttatatccac cgattccggt aacgttagct tccatcactt ctgttttcca
     8881 gccttgtgct tcagcatact tttggtacat attgaaaaga tcgccagcga ataatgctgc
     8941 ttcgtctccg cctgctgctc cacggatttc catgatgata tttttatcgt catttggatc
     9001 ttttggtaaa agcaagatct tgattttttc ttctaagact tctttttctt tttttaattc
     9061 agataattct tcttttgcta gttcagccat ttcatcatcc agtttttcac caagcagttc
     9121 ttctgtatcg gtgattcctt gtactacttc tttataacga cgataaactt cgactgtttc
     9181 gcgcgtgttt gcttcttctt ttgaaagctg cataaaacgc tttgtatcag aaatcacttc
     9241 tgggtcactt aaaagttctc caagctcttc ataacggtct tcaattgctt gtaattgatc
     9301 gtacatgctt attctccttc tgtaatcatt gatgggtgaa agtaatgttt tcgacaaacg
     9361 gggtaataag cttcatttcc gccaatttgc acttgatcgc cttcataaac agggtttcca
     9421 tcaatataat gtaaattcat gattgctttt ttgtggcaga accaacagat tgttttcatt
     9481 tcttcgattt tgtctgcata aagcaataaa tattttgatc cttcaaataa ctcgtttcga
     9541 aaatcatttt tcaaaccaaa tgccataacc ggaatatcca attcgtccac aattctcgta
     9601 aaagcaatga catgtttctt ttttaagaat tgcgcttcat cgatcagcac acaatagggt
     9661 ttgctttcca ttttctgaat tacgtcaaat acgtctgttt cttcaaatat cggcatcgca
     9721 ttacgtttca gtccgattcg gcttgaaaca acgcctaaac catctcttgt atcaataccg
     9781 cttgtcatta agacaactgg cttattttgt tcttcataat tatgtgcgac ttttaggatc
     9841 tcgatcgtct tgccgctatt cattgcgcca tacttaaaaa atagttgtgc catgctcttt
     9901 ttcaccttcc agtttgttcc tatggtatta tacttgattt tcagcatttt ttacagtgag
     9961 aattttcatc aagcacagat tagtattaaa tctattgctc acttttgttc caattttttg
    10021 acaagaacta aacatgggtt ccattcatcc cataaatttg gaaaaacttc caaccttgaa
    10081 aatcctaatg attcgtagaa acaaatagtt tgatcatata tagagtagtg cccctcgtct
    10141 acagtcttca cttgcaaata tttgtaatgt tttgctgcgt aaccttctgc ttctttaaaa
    10201 agttttgttc ctaatccttg gtgatgatag tcttttttta ttcccataca atagaataga
    10261 tttcggcagt atcttcactt gtctgtgaaa gagtgataaa gcctaaatat tcttcttttc
    10321 ttttttcaca ccataaaggt agtttacttg attcctcgat atatttttcc gttgattcag
    10381 gaagcccaaa ccactcaggc aagtctagta atatttctcg gacacacttt cctttttcct
    10441 ctctatcttt tacaaacgat atcggcatat tctttctaga ttctcccttt ttgaattgag
    10501 tatagcatgc tttctttctt ttcacgtgct ataataagtt aaaatttcaa agttttacgt
    10561 aaggagctat ttgatgacaa aaaatatttt tgattccatc gctaaccatt atgattcccc
    10621 tgaccgtcaa gcattagcca agattattcg cgaagaacta actacttatc taccgcgcga
    10681 cagccaccaa aaagtcctgc tggattacgg aggcggaact gggcttgtta gtttaccgct
    10741 tgcggaaaga ttcaaagaac tgatcattgc cgatgcttca gaaactatgc tgaaaatggc
    10801 agaagaaaag atccaagcag cagatctgaa aaatgtccga acgatccacg ctgatgcttc
    10861 tgtcgaattt cctgctgtcc aagctaatct catccttcta tcacttgtac ttttacacat
    10921 tcctgatacg gagaatattc ttacaaaact ttatgaaatt ttagctcctg gcggtcaatt
    10981 gatcatcgta gactttgaca agaatgaaca aattagtcat ccgaaagtcc ataatggctt
    11041 tagccaagaa gaattgaata atcgattgga aaaaacgggc tttgtctctg ctactagtca
    11101 taccttccat cgtggggaaa agctatttat gaacaaaaac gcttcgcttt tcttatccat
    11161 cagccaaaaa gcttaaaatg agattgcaaa aaaagtgagc aactccaaga aataagaagg
    11221 aattttcgga aaaagaagct gtgacaaaaa acatgtcaca gcttcttttt attttttcat
    11281 cacatgattt taatttctga aagaggccaa taacggaaaa caacttttcc ttggatcata
    11341 tctttgtcga ctaacccaaa cattcggctg tcttttgaaa tcaagcggtt atcgcctaga
    11401 acaagatact ttccttttgg cacagtatat tcaaagtcac tcgtaaaggt ggatgaactt
    11461 tctgcttgtg cttggaatcc tgagctataa gcatattctc cttgtaactt atcttctgaa
    11521 aattcttttt tgaactcatc cagataaggt tcatcatact ttttgccatt aatggtcaac
    11581 tggtcatttt ccatcttgac tgtatctcca ggcataccga tgatccgttt aacaatcatc
    11641 cgttcttcat cccccggttc ctttgtagta ataatatcca ttcgttcata gtttgagatc
    11701 ttagatgaaa tcaatcgttg tccatcactt aatgtagggt ccatcgaatg cccgtttaca
    11761 gtaactggtg taaacacgta cactctagct aataaaatag ccgctaatac aaggatcaaa
    11821 atcccccaat ttttcaaaaa ttctttcaca gcttacctcc tacttgtatt ttcctcgtta
    11881 tttattacgt tttttcacaa tacttcgttg tactttcttt attctacaat gtttataaat
    11941 gaaaggaaag aataagtgat gtgttcgtct tatctttctc gttatataaa aaaggaacgt
    12001 gacaaaagac gtgtcacatc ccccttattc cgaataaacg tttttatcgc aaccactgat
    12061 acacggcgtt tcatcacatt atgaatgtct tcttctttgg ctacgactat aaaccactgg
    12121 ttcttcctgc gactgtgcct tttttgcaat caatttcaga cgataaggct catgatagcg
    12181 taacccgtct ttgacaaaat caccgccgac gatgaacaat ccgacaggtt ccaatgtgtc
    12241 ttctgttgag tcaagtcgaa tggtccctac tttttccatg tccgagtagc ttcgtccaat
    12301 atttagaaca tactctttct ctcctgtttt cttgacatag taataaggat acaatgtatt
    12361 tctatcgacc agaaatccat ccatgcgacc gagttcattt gttttgatct caccatttcc
    12421 aatcaaatac cgcatattct cggctaattt caattgataa tcttcgtcat aagaaaccat
    12481 gacctcttgt gtattcactt tatcgatcga atttccatta tctatcgatt cttctgtctg
    12541 gatgttgaac gcttcatcag gtaaatactt cacaaaaagc tgagtgacat cggctttcgt
    12601 gtttttatct aaaaagtaat aaaatacatt taaaacagta aaataaacaa gcactagaaa
    12661 tgcaagtcca tataagaaaa attgaagata gttttggttt tgccaaagtc cataaacaat
    12721 tctcaataaa taaagcgtgg gaatcacact taaaatcgta tatccacggt taagatattt
    12781 aggactgatg tctaaatagc caaggaattt atgcaccccg ttgataaggt ctagtaaaaa
    12841 tgtcattgag aatcacctgc ttgctggtta tacgtttggg tatttcctga atcttggttt
    12901 tgctgtgctg tacgtgctgc atcatcattt tgcgtgttat catctacagt cgtactgctt
    12961 ggtatcgttt cggagctagc acttgtatcc ggacttgtac ttggattggt tgaatccgat
    13021 ggagtgccat ctggtaccgt attacttgtt tcgcttgagc tgtcagttgt atatgtctca
    13081 tcatacgacg aactagttgt tgtatcgtcc catgtacttg tcgtagtggt ttcattcaaa
    13141 ctttccgttg ttgttggttc agatgtcgag actgaagtag ttggtgttgt ttcttctaca
    13201 gtactagaac ttgaacttgg tgcacggtag ctggaactat tcacaatacc tgtagtagtc
    13261 gcgacaatcg tcgagattgt taatacagcg attggcttca aaataggtgc tacttttcgc
    13321 atcttatctc ttccttcatt gaaatttatt cttgtttcct tactttacta cggacaaatt
    13381 atacagtcta ccttatttct accataaatt ctttattttt ttcttacgtt tttccgaaag
    13441 atttctttaa ttttcctcaa aaactacggt tgatgttttt aagtaagctt ttgcttcccg
    13501 attgatttct ttcagatcgc atccttgctg cttggcagcg aacacgcaac cacaataaca
    13561 ttggcgataa atatcatact ctttgcacat ttgcaaagag cgttcatagc ctttgttttt
    13621 cttgaaatca cttggcaagt attgtgtccc gtaaatcttt tggatatcca ttccgatctg
    13681 attgatcaaa gctgcatttt tctttggcga gatggtcagg gcacttccaa agtaatcgta
    13741 tcctagttct tgcgcttttt gcgcaacaag atctaatcgc atattgaagc atgcactgca
    13801 gcgagcacca ccttcttttt cgttttcaag atggtttgtt aaaaccattt tggtgaattc
    13861 atttggacgg tattcatcaa cgattagcgc aacatgatgg tcagttttct cattaaattg
    13921 gtctacaaat cgtttttgtt catgtaatct tcgcaaatac tcacttttag gatgaatatt
    13981 cgaatttgaa aaaaataacg tgatctctgc atattgagac atgaattcca atgtgtgcgt
    14041 actgcaagga gcacagcatg tatgtattaa tatcttaggc tttacttctt ctttctccca
    14101 ttgcatgatc atttttttca gaactgtatc ataatttatt ttttgatttt tcattttttc
    14161 tacgatttgt tgagcattaa tcaatttgtt attcctcctc cttctttttt ctaggatttt
    14221 ccttattata agccatttct attttaatcg aaagctgtat tgttatctgt ttgataattt
    14281 ctttgagtat tcctaattcc ccttgtatac tggtagaaac gactattgtc aggaggatag
    14341 tactgtgcat gttgatttat tctttcgaag gctgcagcgg cgaggacaga agtttgctgc
    14401 caatcatttc tcatcgatcc aaatcatcgt tttttattat atattgatga cggtcttatc
    14461 actcgttcta ttttatatgc cgatttttcg cgaaccagat agccatgttt cttttgtgga
    14521 catgctgttc atggcaatca gtacagtgag tgtgactgga ctatcgactt ttgatatcaa
    14581 ttctgtgttc aatgataacg gtattatact gctggaaata ttatttcaag ttggtggttt
    14641 ggggatcatg atgatttcta ctgcttttgt cattttttct aaacgacgga tcacactgaa
    14701 acagcgacag ctgatcatga cggatatgaa tcaaccgcgt ttgtctggta tcgtccgact
    14761 gattcggatc acttttgcta ttttaatctg gtttcaatta ctttttggga cattcttttc
    14821 tatttatttt tattacagag gctattttga tcgttggcgt gatgccgttt tttacggttt
    14881 ttatcaggcg atatcggctg tcacgaactc tgggtttgac gtcactgggg attccatcaa
    14941 gccctttgca catgactatt ttttcttgat cctgatcatg tttttgattt ttgtcggagg
    15001 aattggtttt cctgtattga tggagtgccg ggaatggcta ttatacaaac gttccaatgc
    15061 gaagcttccg tttcgttttt ccttgtttac caaattagct gtgttagctt ttgttatctt
    15121 gtttgtttcg ggaactgtct tgatttatct attggaaaag gaccatttat tccaagattc
    15181 caatatgagt gtcaaatgga tcaattcgat gttttattcg attacgactc gtaatgctgg
    15241 tctgcagatt cacgatttgg gtgattttca aatcacaaca ctgatcattt tttctttact
    15301 gatgtttatc ggatgcagtc ctagttctgt aggcggcggg attcgaacaa ctactgtagc
    15361 aatcatcggt ttgtatctat attccttcct caaaagtgaa gataacatca atatttttgg
    15421 acggagaatc gatcaagatg atgtgcgaaa atcagtcgtt gtctttatgc tttctcttgg
    15481 tatgtgtttc ttttgtatcg tttttttatc tgctacagaa gaacaaacgc tgatctcgat
    15541 catcatcgaa gtaacttctg cgtttggaac aactggttta tctttaggga ttaccggaga
    15601 cttgtctgtt gtgggcaaaa tcacgattgc tacactgatg tttattgggc ggatcggaat
    15661 gctatataca ttgatgttat tcgtgccaaa agaaacacga gatctaggct atgaatatcc
    15721 aagcgagaaa attattatcg gttaaaatgg gaatataggt aagagggaat aataaaatca
    15781 cagcagcttg aaacaatcat tttgtttcaa gctgtttttt tgttaaatca tgcgattttg
    15841 tccgatttca tgcactttca gccgtaacct tttcttaaga ataagaaaag caatttaacc
    15901 tttttttcaa catatggtat gattagttga atttattcgt cagcaaatcg tttttggagg
    15961 ttacataatg ggaatacgca gtcattttgc gattgcagtt ggaaaaacgt cgcaatgggc
    16021 attaaaaact ttttttaaag gcggatcaag tttaccaggt aaactagcgc tgaaagtcga
    16081 tccacatata ctagatagtt tggcaaaaga ctaccaagtc gtcgttgtca ccggaacaaa
    16141 cggtaaaaca ttgactaccg ctttaacagt caatattttg cgccagcaat tcgatgaagt
    16201 tctaaccaac cctactggtg cgaatatggt acagggaatt gtatcgactt tcttgcaggc
    16261 aaaaccaaaa aaaggacaga aaaagtttgc tgtcctagaa atagatgaag caagtttgag
    16321 caaagtcaca gaatatatga aaccagaact gttcttattt acaaatattt tccgtgatca
    16381 aatggatcgg tatggagaaa tctatacgac ctataaaatg attgtagacg gtgccgcaaa
    16441 gtcaccaaat gcaacgatta tcagtaacgg tgattcacca atcttcaatt ctatcgaaac
    16501 agtcaatcct agaaaatatt atggctttga ccatgaagaa gatcgtgaac aaatggctca
    16561 ttacaataca gacggtgtct tatgtccaaa ttgtcatcac atcttgcatt ataaaatgat
    16621 cacttatgca aacttaggga aatattactg tccaaattgt gacttcaaac gtcctgaatt
    16681 ggattatcga ttaactgata tggtacggat ggacaatgtg tcagcagatt tcgtgatcga
    16741 tggtcatgaa tacggaatcg aagttggcgg tatgtataat gtctacaatg ctttagcagc
    16801 tactgcagtg gcagagtact tcaatgtcga tcctgataaa atcaaacaag gtttaggata
    16861 tgacgagaaa gtattcggcc gacaagaagt catcaatatc aatgggaaaa aatgtacatt
    16921 agtacttgtc aaaaatcctg taggtatcaa tcaagtaatc gatatgattg gtctagctcc
    16981 ttatccattt tctttagtgg ctttattaaa tgccaattac gcagacggaa tcgatgtcag
    17041 ctggatttgg gatggtaatt ttgaaaactt tgccgatatg gatatcccag ctgttatttc
    17101 tggaggagac cgtcatacag atatgacttt acgtctaaaa gttgccggga ttccagaaaa
    17161 taaattgaca gaaacaaaag aattaccaga agtaatcgaa aaaatcaaag aattgccaac
    17221 tgagcatgtc tatattttag ctacttacac tgctgtattg cagcttcgta aagaactagc
    17281 tgctcaagga tttatcaaag gaggaatgaa ccgtggctaa ttatgaactc aatatcgccc
    17341 acctttatgg aaatttgatg aacacatatg gtgataacgg aaaccttttg atgttgaaat
    17401 atatagcaga aaaaatgggg gtttcttgcc atacggagat cgttagtata catgaacctt
    17461 ttgatgctga taaatatgac ttggttttct ttggcggtgg tcaagacttt gagcaattaa
    17521 tcatttcaaa agacatccag aaaaagaaag attctttgat caactatatc gaaaatgatg
    17581 gcgtcatgtt agccatttgt ggtggctacc aattgctagg tcactactac atgggagcta
    17641 atggagaaaa gatccaagga atcagtgcac tggatcacta cacgttaagt caggaaaata
    17701 accggtttat cggtgatatc gttatccata acgaagaatt caatgaaact tattatggat
    17761 ttgaaaacca caatggtcgg actttcttag gtgaaggtga acgacctttg gggaaagtgg
    17821 tacaaggtca aggaaataat ggtgaagacc aatctgaagg tgtcatttac cgaaatgtct
    17881 tcggttctta tttccatgga cctatccttg caagaaatga gaacctggca gctcgtttga
    17941 tccgcctagc tttagaacaa cgttacggta aagaattgga tctgccctat cttgaaggac
    18001 aaacagccta gttattcgaa aaaggggaat gtgacaaaaa tttcgtcaca ttcccctttt
    18061 tcgtatccta ttttaagcga cttgcttggc ataacggttc acgttttgct tgatattccc
    18121 gattgttttc ggatcattga ttcctgtgac ttcttgaatc aattgttcca agttttcatg
    18181 gatcttcatt tcatgcaatt gacggctttg ttcatcttca ggatcatagt aattaaaaat
    18241 aatccccatt gttgccgtca aatgtgggca agtcaagtta tgttcttgta gttctctcac
    18301 tggacgtgta aatctttctt gataacctaa cttacgtaat ggtgtccgag cgacacgtgt
    18361 gatcgcatca gagatatttt tgttttggaa gcgatgaatg attttttcaa tatacgcatg
    18421 atgctgctct gcatcaaacc cccatttggc aatcagcaaa cttcctgttt cctgcaaaac
    18481 tgattttaat tgtgccacaa ctaacgcatc ttgcattgct tcatcaatgg tctggtaacc
    18541 taacaaggca cctgtataag cgacagtcgc atgaccagta ttgacactaa atagtttgcg
    18601 ttcgatatat ggttctagat catctacata taaaacccct tttaattgga tttttttatt
    18661 ttttctttgg ctatcgtcaa tgacccattc tttgaaaggt tccacttgga cgaataaagg
    18721 atcttcatgg tgttgtaatg ggacgattcg atcgactgct gcatttggaa agccgatata
    18781 tttgtcagta aatgatgttt ccggaagata tttatacact tctttttcta gaaatgctga
    18841 accgccaatc atattttcac aggcaatgat atctaaagat gttgtgcttc cttccttttc
    18901 tctttcttgg ataccttttg caatcaactc agcaataaaa ggtaagatat tcgggccaat
    18961 ggcggtagta actaaatctg cttcagcgat ttcttggatt actttttctg gatttgtttg
    19021 attgttgatt cctttgacat tttttacagt gattttttct tttgattcat ctgctaattc
    19081 gatttcgtat tcttttcttt catttaaagc atcaatgatc gtgtgattca catctacgaa
    19141 agtaatctca aatccattag ctgccaaaac ctctccaata aatcctcgac cgatatttcc
    19201 tgcaccaaaa tgtactgctc tcatttttct ctctccttaa gcaattgcta gattttccgt
    19261 tacttcttct ttgctgagcg cgtctgctag ctggacaaca ttgtccatat cactgcaata
    19321 aagtgcgatt tgctgtacaa gttgcaaatg ctcatctccg actcctgcaa taccgaacaa
    19381 aacagtcgca atcttttctt cttcttcggt accaaaatct acaccatctg gtacttgaac
    19441 gacacagatg ccggattttt tcacatattt ttttgcttca tctgttccat gaggaatcgc
    19501 gataaaattc cccatataaa cagataacat tttgtctcta tctaccatgg catcgatata
    19561 ttcctcatca acgcaccctg cttctacaag catctgaccg caatagcgga tcgcttcttc
    19621 tttggtttta aaagatcgat tgagctcgat catgtcgaat ggcaaacttt gcatgctgtt
    19681 ttcctccctt aagctgtctg tttcaaatat tgtgcaatcg attcgtattt ttgcggattg
    19741 accatgctgt tgactgataa atgaatggat tgaggtgcag ccatctgtac tttttccgct
    19801 aaactttctt tggctacgat cagtgaatca ggttcatctt ttaaatcttg gatattgact
    19861 tttcttaacg ggatagttaa atggttcttg tctaatatat ctttgagcac ggtcatcccc
    19921 attgttgctg atccggaacg atcatcgtgg acaagtagaa tctctttgat ttgtttgatg
    19981 tttgctgaaa gaaactcttc tgctggctca ttctcaattt cagaaactga tgttttctgc
    20041 ttgctgctta attcttgaat gatttcatca tatcgaggtg aattcaaaaa gttttctact
    20101 gagacaaaac gcgcgctcgg tgctttttgt tttgctcttt cttgtagttc gacctgagta
    20161 acgatcaatg tattcgagtc atctgtcagt tggctgattg cttgattggt caccgtttga
    20221 ggcaagtctg cttcctgtac tttttttctc aagatcgaag cgcccatcgc acttgatccc
    20281 attcctgcat cacaagcaaa aatgattttt tgtacgccag agaatgtagc tccttgagga
    20341 ataccttggg cacctttgga agcttgctta gctgcctttg tctcttgaac agatttttcg
    20401 aagtcttcat ttgtatcttt cgcatccgct ttcaaaatta ctgcagaaat accaaacgaa
    20461 acagctgcgg caacaatcac gcccaaaata acaggaaggt aagaattgaa cggtgtcatc
    20521 gctagaatcg caatgatcga ccctggagaa gctggcgctc ttagtccagc atccatcaat
    20581 tggaaaatga aacttcctga cattcctccg cctataactg ataagaataa taacggtttc
    20641 atcataacgt aaggaaaata gatttcatgg atcccgccta aaaagtgaat gatcatggct
    20701 cctggtgctg aagattttgc tgctcctttg ccaaacaaag taaaagcaag taatacgcca
    20761 agtcctggac caggatttgc ttcaagcaag aatagaatag attttccagc ctctgctact
    20821 tgttcagttc ccaatggtgt caaaatccca tgattgattg cattattcaa aaataagatt
    20881 tttgctggtt caataaaaat acttgtcaat ggaatcaaat gggcagataa aatgatctct
    20941 acaccttttg ccattgcttg agtcaatgca tcgacaactg gaccaatcgc agaaaaacct
    21001 aataacgcta atccaaaacc gataagacct gctgaaaaat tattgactag catttcaaaa
    21061 ccggatttga tccgtttttg gaatagctga tcaaatttct tgatggcata tccaccaaga
    21121 ggtcccatga tcatcgcgcc tagcatcatt ggaatatctg ttccgacaat cacacccatc
    21181 gttgcaattg ccccaacaac tgatccgcgg tcaccagcaa ttacttttcc gccagtataa
    21241 ccgatcaata aaggaatcag ataagttagc attgggctta ccatggtagc gaaagcctca
    21301 tttggtaaat aaccatctgg gataaataga gccgttaata caccccaggc aatcagtgct
    21361 ccaatatttg gcatgaccat gcttgataaa gtactgccta atttttgtac cctcgctttt
    21421 agcgaaatgt tgtttttggg aatcgtccgt tccattttgt ctctccctct ctccttttga
    21481 tactctaatt gtatccgctt actaaaaaga gaaatagtcg tactttggca cgaaactttg
    21541 tgccaaaggt ttgagtatgt caacaaaata aaaagatttc ggtatcagaa aacttcaatt
    21601 ttaaataaaa ctgttatttt aattttttta taatatgcaa acaataactt ttgttagcat
    21661 ttaatttaga tgttttccat tctattatgt tattaaattt tatattggag gcctatctat
    21721 gacattactg atcaaaaaca tgaacatgaa acgtggtaaa aaacgtgtga ttcgacaatt
    21781 gaatctttta gtagaatcag gagaagtagc tgctttgatt gccccgaacg gatttggcaa
    21841 aacaactttg ctgaatggca ttgcccaact gctgccaaca acttatgatc aattgtccct
    21901 tcacaacatc tctcctactg cttatacaga ttatcgccgg tctttctttt ttctagaatc
    21961 aacgaatcag ttatccgact atctatcggc tgttgattat ttgactttga tcaaatacta
    22021 ttggcagagc aaagtagaat gccaaactgt tttccaacga ttgaagatga ctgagtacct
    22081 tactttgccg attggaaaaa tgtctctagg aatgaaacaa catgtcttgc ttgctgctta
    22141 cttgatcagt gattcatctg tcatgttatt cgatgaaccg ttgaacggat tagacccagg
    22201 aagtattgat atcttgaata gcctcatcag acaatggcag aaagagggga agacgattct
    22261 tctttcttcc cacgctatcg cgaatatcca atcgatttgt tctaaagcat gttttctaaa
    22321 aaatggagaa atcgtcacag aatcctcaaa tatggaagag attttagcta tttatcgtaa
    22381 attatatgag gtgtaactat gtctttactt acatttgaac gcaatctctt actaaaaaac
    22441 aaagtaaact ttttatttcc tctattgttg gtcgtgctct ttgcattccc actttttttt
    22501 gatcataaac ttgcttatac agaattcgat gaactgaatc ataactatga agagatgcaa
    22561 cgtttgattg aaacgttgaa agaagatgag aatgagaaag aatttgtgga aagtttagag
    22621 aaaagcaaca aactgatcga agcaatctta catgcaaaaa acacagggaa tgtccagcaa
    22681 atggtcgaag caacttatca ttatgaaaaa gatatactgg atcgactgat ttctggtcaa
    22741 agacaaggaa ttccaatcat tgaacaacaa aaacgtgtcg agttgctccg ttatatgaaa
    22801 gaacatcaga tccaaagata ttctattttt gacttgccag cgcatctctc tttagcaaat
    22861 tactacgaaa atattttttc aggaatgatt tctagttttt tgattttatg tataaccgcc
    22921 ttgtttcttt cttccatcat ctcttatgaa aaaagaaagc aggtaatctc tctagtcaat
    22981 ctattaccgg atagtatggt aaagaaacat agcatccgtt ttaccattta ttatggtgca
    23041 gctatgctaa gtctcgtgat gccatttttg atcgtctcta tactagtaat aatcaaaaat
    23101 ggtttgggag acttccgtta tcctgtagga acgatcattg gtcaggagat acgaatcctg
    23161 ccgatgtatg agtatctttt ccagagcttt ctgttcctgt tgttgtgggt gctcttttta
    23221 agtacaatca gttttcttct atctgcttta tttgaacatt ctctagtcaa tcttttaggt
    23281 acacttcttt gtcttttctt agcagagtat cgcttgtttt cttcgattgg atggatagaa
    23341 tcaatcagtc attacttgcc aacttcttat gttgattttc aaaatgtgat cataggagga
    23401 gatattttct cgccactagc ttcagaacag gttactttca tgaatggtat cttgacactt
    23461 ggtatatgga gtatcgtttt attattcata ggtatgggca ctatctatat aaaaaagagc
    23521 tattaaaaca caaaaataat aggggcggtg acaaaaacct tgtcaccgtc ccctatttca
    23581 aataaacggt tttctagaat aaatagtttt caaaagcttg catccgcagt aataagaacc
    23641 tcccaacaaa aatagctcct cttattttta tcttggttga agttattact tgatgcaagg
    23701 cagctttttt tactgtgaac attggttata tatgtccatg ttcaaaaatc atttggactt
    23761 cttcaatggt ttccgcatca ctgagcgcca ttacttggtc gatgtctgaa cagaaaaagg
    23821 ctaaatcttg gagcaccgct aactgatgtt ctttttttaa agccactaca aataagagtg
    23881 tcgcgatttt tggttcctct tttgttccaa agtccacacc atcaggcact tggactaaat
    23941 agattccttc ttctactact tcttcatctt tgtcagtccc atgtggcaag gctacgaaat
    24001 tcccgatata gacactcgct ttttcttgtc tttcgatcat tgattggata tatgccggtt
    24061 tgattccccc ttgttgggtc atccactggc cgatctcggt caacgcttct ttccaatttt
    24121 gataggattg atcgagcaat attttttctg ctggtagctg tatcatcttt tcaccttcat
    24181 ttctttattt cttcaatcag gattttcgct aacaactgat aaacgatttc tttatttcct
    24241 gagcggaaga tctcggtgtt caagtcattc atgatgattg ctccactgat ctttcctaaa
    24301 agaatcccgt ctttttcttg gattggcgaa ggcgccagca taactaacat acgggacaaa
    24361 tcgactggct gtctatccat cccttcaatg gtcaacggat gtttcaaatt gaaaatacaa
    24421 aagatcggtt tagaaatcaa tgagctggat gtatggaata accccatatt tgtatgagga
    24481 ataccgatag gtgcttgttc atatcgctta agcagccggc gatgaaccac ctcttcatcc
    24541 gttactagtc cttcaggaaa ataaggaaat aagctgcgaa tcgtttcgga aatcgtctca
    24601 ggattatcca gttcttggat aaaaaaacga ccgagtaatt gattgatttg atccatgaaa
    24661 tgcattgttt cctcataagt atcttctatc atccactcgt tattgctgtt atacgtaatt
    24721 ttttgccttt ttttcttctc aatatgcgaa aaagcttctt ttagctgctt gacttcatct
    24781 tctagcagta gcggggagac taaaatatat tttccaccgt atcctggtaa aatagatgta
    24841 gaaataacaa catcatattc ttcttgtaaa tcgatttttt ttaaatcagc aatccgatag
    24901 aaatgaattt gttggatgaa gggaaaatac cttttcaatc gtagttccaa catgctagta
    24961 gaagctaatc cacttggcga aaatcctgca atactgatat tgctttcttt cggacttcgt
    25021 tccaatgaat tcgcaaaatg aaggaccata taagcgatct cttcttcgga taattttttt
    25081 tcttgaaaaa tccgctctac tgctttacgg attgcattcg caacttcctc gtattgaacc
    25141 atgatttttt ctaaaatcgg attggtcagt gtctcttctt gtaaaacggc tcttgaaaac
    25201 acaccagata aatgagtcaa cagcattttg tacaaagttt catcttcata aaaatggaca
    25261 ttcgtctctt cacttacttc ttgaatcagt tgttttacta aataagcaag ctgtgtatcg
    25321 aacgtctctt caaagaaatt ctgatcaaat gagttcgtga agtcatttaa taatcctgca
    25381 aaaaagacga tctcatttac tggataaagt tgttttgtct cagaagcgat ccgtgaaaac
    25441 aatcgtttag ctgtctgtag taattctttg tttgccagac tattatatgt ctcatttgtc
    25501 agtaaatgat ttttcgtcac acgctccatc gtcaatgtca gcatcaaaac caaatgttca
    25561 agctttctat cacttagatt ggtctttttt tggatcaatt cttccccgat tactttgcgt
    25621 gcaagagata gatgttgcgg atcaacgaat cgaaaaaaat aattcagtga tgcatcgaaa
    25681 gaaatactat gaaaaagttc atattcattg atttctaatt ctaatacatt cgctgtcaga
    25741 agccggcggt atttttccgg tccttcgatc tcgtatccct ggttccgaat gatttccaaa
    25801 ggcagttggc gaatactttc ttccaactgt ttgatatctg cataaaaagt ggtattactg
    25861 atgcgatatg attccatgaa atgagcaaga ctcaacggct ctgtcgtcag taacaactcg
    25921 gctaaaatcg cgtgttgtcg tttagcagtg gataagtcat tacttttccc atctgtattt
    25981 tcttttaatt gctctaatac ttcttctgat gcctcaatga caaatgagcc tctagctgct
    26041 tttttcaaag aagcatttac cgatttcaat gagacttcaa gattatctaa ttcgcggtaa
    26101 atagtccgtt tactgacctt caataaattc atcatttttt ggatagatac aggtgacggg
    26161 ctactgagaa attctgctaa taattgtttt tctctgggcg ataaatacat cttatatttc
    26221 tcccttcacg agtttttcgt attcattcgt agctacccaa tgttcaacta gaactagccg
    26281 agggatcttt tcttctagct gttcagttac agctagttct ctttgtgcga tataaatcgt
    26341 ttcttcttgg attttgattt tttctattga ttcttttgag ataacaaact gttttttcgc
    26401 tttttttgct aattgactta aaatcgtcat gcccatagtt tgtgagccgc gtctgttgtt
    26461 tttatacaaa aatacaatat tagcaggcga cgttttttgt tctgtgatat ctgctatctc
    26521 ttttttttct tcccgctttg gaaattggat cgcttctttt attcgtgaca gcaagatatc
    26581 tgtttccaaa aagtgatcca gtacatagat ttgactttga ggtacttttt ctcttgcaag
    26641 acccgctagt tcatgttgca cgataaccag ctgatttact tggttattca attcatagat
    26701 agagctgtat ccgacagaaa gatcgatttt ttctgctgct aatttctttt tcagcagggc
    26761 ggctcccata gcacttgatc ccatacctga gtcgcaagca aaaatgacgg aatgaatgat
    26821 agatggttcc aagatttctc ccctttcttt gaccgttttg ttctcttttt ctttatttgg
    26881 taatttcaat agtatcgctg cacaaatgaa ggtcacaaat gtactgattg ctatccctat
    26941 acttacccca gctagttgtg gataaggcgc attggcaaaa atcacgagta gtgacccagg
    27001 cgatacaggt gcagctaatc ccgtatctaa ccattgaaat atcaacgtgc cgcttgcccc
    27061 accagccatt acagctaaaa atagatacgg tcgcagcaag acaaatggaa aatagatttc
    27121 gtgaatcccg ccaatcgcat ggatcatcaa tgctccactc gcatttactt tttcctgttt
    27181 ttctgcaaaa caaaggatag aaagtaagat ccctaaacca ggtccaggat ttgcctccat
    27241 caaaaataag gaagagtacc cgactgtttg tgagaattcg attcctaaag gtgtcaatac
    27301 accatgattg attgcattat tgaaaaatag tacttttaga ggttctaaga tcagactgac
    27361 aagtggtaac agattcattg tgactagcca agacacaacg gaggcgagca acaagtgaac
    27421 actatttaaa actggttcaa taaaaacgat tccaattata caaataacgc taccagtcag
    27481 tcctactagt aggttgctga caagcatttc atatccagcc ttgaatttca catggaatct
    27541 agaaacaaat tgttcatata cgatgccggc aagcggcccg ataatcatcg ctcctacgat
    27601 ttgtggagca ttggaacgag cgatcaaccc taaagaagca attgcaccgg cgactgcccc
    27661 tttttgtccc cctaccgatt tcccgcctga ataactgatc aagatgggta aaagcagctg
    27721 gatcatccaa tggcatacct cttctaatgt ggtgtgcaaa tcgccagaca caccttttgt
    27781 tgctaatgac aagattcccc aagcgataaa cagacttaga tttggcatga tcatttcatt
    27841 taagtgatgt ccagcttttc tcagttgttt catagacacg cgactgctgt tgtttgcttt
    27901 ccatttgtat tccatctttt tcaccagact tctatttatt gatggacgga ttttatgtag
    27961 aaaaagcagc aaacgaaaaa ccgtctgctg ctttcagcta tctgggaact atttgcctgg
    28021 ttcagacgca atgattgcta gttcaccttg gaccgcccac tcagaaacgc catttggaat
    28081 aatacagctt gttccttttt ctaatggata tgtttttccg tcaacgacaa ggtctcctgc
    28141 tccttcgatt accgtcatca atgtataagg ggcttgtttt ttgaaagatg tgattccttt
    28201 aatatcccac tcatatacat tgaaaaactc agtttctaaa taagtgacga tcgatgaatt
    28261 cccttttcgg acttctttga tttgtaattc tggtgttttc gcagggacag ttgttacatc
    28321 gatcgattgt tggatatgca gctctcttgt ttgtccttgc gcatcttttc tgtcgtagtc
    28381 atacacgcgg taagtcgtgt cagaactttg ctgtgtttct aaaatcatga ttcctttacc
    28441 aatagcatgg atcgttccgc ttggtacata aaagaaatca ccttttttaa ctggtacttt
    28501 cttcaaaagg tcatcccagc gcccatcttt gatcatctca gctagttctt ctcttgtctt
    28561 tgcatgatga ccatagatga tttctgcacc aggttcagca tcgatgatat accagcattc
    28621 agtttttcct aattcacctt catgtttcat cccataggca tcatctggat gtacttgtac
    28681 agatagatca tcttctgcgt ctaagatctt gatcaataat ggaaacactg gttctgaagg
    28741 atggccaaac aattccttgt gttctgacca tagttcatct aatcttttcc ctttgaattc
    28801 gccattttct actacactga caccatgagg atgcgcactg atcgcccaat cttctccgat
    28861 tttatcgtta gggatatcaa aaccaaagac tgaacgtaaa cgacttcctc cccaaatttt
    28921 ttcttgaaaa actggtttta aaaacatagg ttcctgcata attcaaactc ctctcatttt
    28981 tcatagctat tatagcatta tttcggtaat accgctttct ctttcaaaaa gttttcaaca
    29041 atttcatctc gacatatgag atactccgca cgatgatcga caggatgtac gcgatttgac
    29101 aaaaatatca acgcttcttg tgtttttata tcaatcaaga aaaacgtacc tgtataacca
    29161 gtatgaaaaa gaatcgcatg accgtcttct acatcctttt ttaagtccca tcctaaagaa
    29221 cgatagccat tttttttcgg tgtttgatct tgcagtaaag aatcaatcgt ctcttcttct
    29281 aaaaaacgac cattacggat ttttcccttg ttcaaataca tttggacaaa agtaatgctg
    29341 tctttcaaat caataaagag acccgcgtta cctccatgtt cagccaaaac tcttgctttc
    29401 ggatcatgag tttgtccacg caaaacctca cccgaaggca gttgttctgt gggaacgatt
    29461 tttttggaag gaaaaggaag aaataagctg ttcatcattc ctaatggttc caatacttgt
    29521 tcttgaaaga tttccactgc agtttcttgg taaatttcct ctagcatcaa cccaagaaga
    29581 atcgtaccgg catctgtata ttttacttct tttcctagtt gctcacctgg ttctagcttc
    29641 aaataagcgg cgatcaattc ttcctgagat agcttatctc tgtttggtat ccatgttttg
    29701 atatcagaag tatgtgtcag taaatgacgc aaagtgattg tctgattttc aaattcagga
    29761 agatatttat gaagcggttg atcgagttct atctttccat cttctaatag acgtaaaact
    29821 aatgaagtgg ttgcaacgac tttcgtcagt gaggctgcat caaacaacag atcttctgtc
    29881 atcagctgct tttcaggaac gacttgggca aagccggctg taaatgtttc tacttgattt
    29941 tcttttatga aggcgactgc tacacctgga taaaatcctt cttccatctt tttcaaaata
    30001 agctgtttcg tgtgtggata catctttttc tcctgctttt ctatcagatt cttacagtcc
    30061 aagcttaaag cttaatctat gaggaatcaa ccttttttac acaaaaaaac tgcgcgaaaa
    30121 accgcgcagt ttcccccggt atactatttt ctctttctat ctctagaata agcgatagta
    30181 tgaccgttca taaaatgacg agacatgcga caatcgttaa gaaagaaaaa gttaagcgtc
    30241 gcatcgaatg tcagttttat tcttttcttt tggctcactg acgaaccacc attcgattcc
    30301 tatagcatca tagattgtga gtactgtgtg ctttttgtct aagaagaaat caactgatag
    30361 ttgctgcaag tgagcaatca gtccttccat gctttcagta tcgatcatga atttcagaaa
    30421 atcaagtcca atcacttcat ctgtaggcaa atcaattgcg cctccgctag cttctgtcaa
    30481 accaacatgg aaatctcctt cattcaatac atggatgccc tcttcttcat tatgtacctt
    30541 caaaccaagt acatgattaa gaaattggcg ttcttttgtt aagttagtaa catttaaatg
    30601 gattttttca aaataagaac ctgttgatag tttactgaat ttttcttcag attttcttaa
    30661 taatgcttct tgatccattg tttctggaat ttctctttgg tcgttcttat tctcttcatg
    30721 gtaaatttcc aattcattct cttctgggtc tacgagcaaa atccccatac gtccatgatc
    30781 ttctaaagct tctttgattg gataattcat tttacggata cgacaaagga tatctgccat
    30841 ttcttctgtg ctagggataa tcaatgaaaa acggttcagc tttttgatca cccccatgtg
    30901 atcattcgct ctaggacttt cttccagcag caacatttca cttccagctt cttctgttcc
    30961 taaaatggct aattcgtttt cttctctttt caaatcaaag ccaacaacgt tcttgtaaaa
    31021 atcaatcatt ttatctcggt ctttcacccg aatggctaca ctttttaaat aggtggcagc
    31081 ccccaattga aaatctttca tatttcttgc tcccctctac gtcaagcttt attttaacga
    31141 tggattcttc ttctttcaag caataacttt gattttgtta aaaaaaatgt gagaaaattc
    31201 gcattttgct tgttttttgt aaattaatga gtaaaaactc ttataaaaag aggttgtgaa
    31261 aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagctcct cctatttttc
    31321 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg
    31381 tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt
    31441 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt accaagaggt
    31501 tgtgagcctg ccgtttaact ccgagtcaca aggagcaatt tcaaaaaaca gcttctcagg
    31561 tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt ttaaggaaga
    31621 ggttgtaaaa tcagcggttt gacctgagta ttagagatac aaaacggaaa aatagcttct
    31681 catattttgc cgttttttga gctaataccg aaggacagct gattgaacac cgtttattcg
    31741 gaaaaaggga ctgtgacacg acttttgtca cagcccctaa aaaaaataat aaatttgtac
    31801 tattctatga agcttcttct tcagaaaact gactgttata aagatctgca tagaatccgt
    31861 ctttttccat caatgtatcg tggtttcctg tttctacgat cgaaccatga ttcatcacaa
    31921 taatgttgtc tgcatcgcgt attgtagaca agcggtgagc aacaacgaaa cttgtccggt
    31981 tttccaatag gcggttcatc gctttttgaa tcagtatttc tgttcgtgta tctacactag
    32041 atgttgcttc atcaaggatc aggacatctg gatttgctaa gaatgcccgc gcaattgtga
    32101 ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc gtttaataca gtgttatatc
    32161 cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc tttagcagcc tcaatgattt
    32221 gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc atagatactt cctgtaaaca
    32281 gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa ttcatcacgt gaaagatcac
    32341 gggtatctac accatcgtaa cgaatactac ctccagaaac atcgtagaac cgttcaagaa
    32401 ggttgatcag tgtcgtcttc cctgcaccag tcggacctac aatagcgacc atctcaccag
    32461 gtttgacatc caagttaaaa tcagtcatta gcaagttatc tggtgtgtag ccgaattgaa
    32521 cattttcaaa acgaactttg tatggtgtat ctttttcttc tggaaggcca gatttttcat
    32581 tggacatctc ttcttcatcc aacacttcaa atacccgttc ggctgaagca actgttgatt
    32641 ggatagtgtt catcaaactt gcaagttgag tgattggttg agagaactgt gttgtatatt
    32701 gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt tgcaactttt actccgccta
    32761 gtacagcgac gaacacatag cctagatttt taacgaaatt catcaacggc atgatgattg
    32821 ccgaaataaa ttgagctttc caaccagcgg catacagatt ctgattttct ttttcaaata
    32881 cttcctggtc tttttcagta tgattgaatg ttttaacaat ggtatgacca ccatatgttt
    32941 cttctacttg gttattcaat aaacctaaac ttttttgctg atctgcgaaa aacacttgtg
    33001 atttcggcgc tacgatcatc acgatgatca agcttaaagg gatcatcaat aacgcgacta
    33061 acgtcaattg ccagctgatt gaaagcatca tcacgatgac cccaatcaat gtgactgcac
    33121 tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc gatgttatcc atatcattga
    33181 ttgctcgaga catgatatcc ccatttgtat gtgtatcata ataggaaatc ggtacacgat
    33241 tcattttggc ttccaaatca tgacgtaatt cataaactgt tcgttgggaa acacgtgtca
    33301 taataaattg ctgtaaaaag ttgaatacag cagaaatcaa atacatcacg attactgtcg
    33361 caatgatctg cgcgatttta tcaaagtcta tcggaaattt tgttacttgt tggccttgtt
    33421 gcatctgttg agcgccagcc attacacctt tgaatatttc tgtggttgct ttccccaaga
    33481 cctttggtgt ttggatttgg aagatcacgg ctgctattgc tagtacaaat acagcgatga
    33541 tcgcaaccat acgtttagac atatagccaa acaatcggcg taccgttccc cagaaatttt
    33601 taggcttttc accttttaca ccgagatttc tgccaggtcc accatgtcct ggaccgcctg
    33661 ctggtccatg agagggacgt gtgtttgttt tactcatgct aagtcctcct ctcttagttg
    33721 tgaatgaacg atttcttgat aagtttcgtt tgttgctaat agttcttcat gtgttccttt
    33781 gccaaccagt tttccttcat ctagcacaag gatcatatct gcatccatta ctgtacttac
    33841 tcgttgagca actaaaacgg tgatactttc tttcatattc gttttcaatg cttggcgaag
    33901 attggcatcc gttttgaaat ccaacgctga gaaggaatca tcaaacacat agatatctgc
    33961 tgttttgact aatgcccgtg caattgccag acgctgtctt tgtccaccag agaagtttcc
    34021 tcctccttgt tccacatggc tatctaagcc atcttccatc tctgaaacaa aatctttagc
    34081 ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt gcatccggct taccatactg
    34141 catattgtct cggatcgttc cagagaacag aactgctttt tgaggaacaa agcccattaa
    34201 ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt ccatttattt ggatcgaacc
    34261 agattcgatg tcatataatc gagggatcaa attgactaag gtcgttttac ctgaaccagt
    34321 cccccctatg attgcgacga tctcgcccga cttggcttgg aaatcaatat cttccaatgc
    34381 aagtttttct gctccatgat atcgataatt gacatgattg aaggccaatg ttgcgttttt
    34441 tccagcaaaa gaaactgttt taggattttc agggtcatgg ataccgattt tttcatccaa
    34501 cacttcatta atacggtcag ctgaagcttg tgcacgaggc accatgataa aaatagcgga
    34561 aagcatcata aagctgatca agatctgcat cgcataagtc ataaatgcaa tcagattacc
    34621 gacttccaaa gtcatatctg ctatgtagtg tccaccaaac caagtgattg ctacgtttgt
    34681 accgctcatg atcaatgtca tcattggcat catcaatgcg acgatcgtat taactttgat
    34741 tgccgtattt gtataatctt tattcgctaa atcaaaacga ttttcttcaa agcgtgtctt
    34801 atcgaatgct cgaatcactc tgacccctgt caatccttct cgaaatacca ggtttaaacg
    34861 gtcagttttc ttttgcatac ttttgaataa tggaacagca aagtacatga ttccgccgat
    34921 caaaataatc attactggga tgacaactaa gaagattttt gtcaactgat gatctttttg
    34981 ataagcaagt atgctcgcgc caatcaacgt gattggtgca ttgatcatca tacgtaaaaa
    35041 catttgtgta accatttgga tttgagtcac atcattggtc gttcttgtga tcaaagaggc
    35101 cgttccatat ttatcaaacg catcttttgt cagactctct gattttttat agacatccgt
    35161 acgcaattgc ttacccagtt tctgtgattc acgagtagca aaaaatgtat ttgcgatagc
    35221 agctaagata ctgatcaaag aaaagccgat catcacaaaa cctgtatgcc aaatataatc
    35281 gacatctcct tgcgctaccc ctttatcaat aatattggag gtaagtgtcg gcaaatataa
    35341 atctgccagt acttggatca ccatgaaaac tgcagctgca attgctgaag tcaaggagat
    35401 ccgttttacg atttttagca tctagttccc ttctttctat ttatatactc cgtttttcag
    35461 ccaagaaagt ttcaaattga attcactgat tgcttgctct actgagtaat cttcagatgt
    35521 tgaaaataag cgatacgaat gcgcaattgt tgtaaataca gtattcatca ctaattggat
    35581 cagtaattcc aattcatgat cattttttac tttcagcttc gtccgatcga tcaattcaag
    35641 caactctttt gcatcttgtt tgtgttcttt atgtggatcg tgaggcgttt tttggcgact
    35701 cgtatgagga gcgacttttc ttgaggcacg gtaatccatg ttcataaaca agttacgata
    35761 aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt ttagaaaaat aggattcgaa
    35821 tcctttgaat agatcaccat cggattcttt cattagttgt aacatatctt tagagctgtc
    35881 acgtctcatt gtctcaaagt aataaaaata aagatcctct ttatcttcaa aatactgata
    35941 gaaactgcct ctagggatat ccgctaattt gacgatatta gcaatcgaag cttcttttaa
    36001 gggtactcta gaaaactcga ttctcgcagc ctcgattagt cttttttgtt tttctttagg
    36061 caaatggaaa aatgtctgtg tcggcatatg gattctcctt tctttcaaga tgacacattg
    36121 tcatatgacg ctatgtcatt ataacacctc ctacctgttt tgcaagacat ttgcatagtt
    36181 tttttcatta tcatccgata cattaaaaaa gtgataagag gaatccttcc atttgaatta
    36241 atgagagttg cccattaaaa tagccacata ctaaacaaag gatgagataa atggcccaat
    36301 caattagtgt ccaagacttc aataaattgc ctctcgatgc tgacagcatc gttttagata
    36361 caagaaatac cttagattac acaaaaaacc acttaagcca ctcgctttct atccccgctg
    36421 ctatcttgcc aagacaatta aaaaatctag atccaaagaa actttattat attctaagct
    36481 atacaggcag gcgatcagaa gcattagccg atcaacttac tgctaatggt tttcgtgctg
    36541 tttatatcat tggtgggatg gaagctcttc agtcgagtgt tgcttgaata ttgcttgtta
    36601 gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct cttttatacc ctgtcttttt
    36661 ttagttattt attcttcaac cttatcaata tgacttgata gccttttctt tttcgagtaa
    36721 actgattttg ttagtcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    36781 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    36841 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    36901 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    36961 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    37021 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    37081 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    37141 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    37201 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    37261 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    37321 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    37381 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    37441 cttcaaagca gcatttgtgc cttggatatt ttcccatctg tctctttttg ttattctttc
    37501 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    37561 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    37621 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    37681 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    37741 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    37801 atcatcgcta aaaatgatcc taagtaaggg atcaggttta aaaaacccgc aataatcccc
    37861 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    37921 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    37981 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    38041 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    38101 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    38161 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    38221 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    38281 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    38341 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    38401 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    38461 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    38521 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    38581 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    38641 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    38701 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    38761 aagcatcaaa aatcaatgat acgtgagcta ctgtctttaa attcccattg gccatcggac
    38821 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    38881 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    38941 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    39001 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    39061 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    39121 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    39181 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    39241 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    39301 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    39361 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    39421 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    39481 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    39541 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    39601 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    39661 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    39721 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    39781 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    39841 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    39901 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    39961 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    40021 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    40081 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    40141 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    40201 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    40261 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    40321 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    40381 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    40441 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    40501 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    40561 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    40621 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    40681 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    40741 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    40801 cccattgctt ttgctggtgg acgcaaagta ctgactttca taagcattgc ccgatatttt
    40861 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    40921 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    40981 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    41041 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    41101 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    41161 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    41221 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    41281 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    41341 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    41401 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    41461 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    41521 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    41581 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    41641 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    41701 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    41761 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    41821 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    41881 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    41941 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    42001 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    42061 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    42121 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    42181 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    42241 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    42301 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    42361 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    42421 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    42481 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    42541 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    42601 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    42661 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    42721 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    42781 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    42841 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    42901 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    42961 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    43021 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    43081 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    43141 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    43201 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    43261 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    43321 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    43381 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    43441 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    43501 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    43561 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    43621 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    43681 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    43741 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    43801 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    43861 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    43921 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    43981 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    44041 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    44101 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    44161 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    44221 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    44281 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    44341 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    44401 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    44461 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    44521 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    44581 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    44641 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    44701 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    44761 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    44821 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    44881 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    44941 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    45001 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    45061 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    45121 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    45181 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    45241 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    45301 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    45361 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    45421 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    45481 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    45541 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    45601 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    45661 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    45721 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    45781 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    45841 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    45901 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    45961 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    46021 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    46081 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    46141 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    46201 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    46261 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    46321 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    46381 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    46441 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    46501 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    46561 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    46621 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    46681 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    46741 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    46801 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    46861 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    46921 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    46981 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    47041 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    47101 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    47161 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    47221 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    47281 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    47341 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    47401 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    47461 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    47521 tccctgtccc tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    47581 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    47641 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    47701 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    47761 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    47821 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    47881 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    47941 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    48001 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    48061 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    48121 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    48181 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    48241 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    48301 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    48361 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    48421 attattgtat acttttgtac gtacatgtca agcagttatt tacaagaata caataaaaaa
    48481 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    48541 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    48601 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    48661 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    48721 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    48781 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    48841 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    48901 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    48961 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    49021 ctggcatgct cctttcctat gttgcagatt atttgctaaa agggttgcct gaaacgatgg
    49081 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    49141 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    49201 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    49261 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    49321 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    49381 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    49441 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    49501 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    49561 gactttcttt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    49621 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    49681 tgacatgggt aatcgtaggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    49741 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    49801 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    49861 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    49921 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    49981 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    50041 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    50101 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    50161 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    50221 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    50281 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    50341 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    50401 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    50461 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    50521 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    50581 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    50641 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    50701 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    50761 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    50821 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    50881 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    50941 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    51001 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    51061 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    51121 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    51181 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    51241 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    51301 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    51361 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    51421 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    51481 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    51541 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    51601 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    51661 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    51721 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    51781 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    51841 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    51901 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    51961 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    52021 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    52081 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    52141 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    52201 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    52261 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    52321 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    52381 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    52441 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    52501 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    52561 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    52621 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    52681 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    52741 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    52801 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    52861 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    52921 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    52981 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    53041 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    53101 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    53161 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    53221 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    53281 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    53341 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    53401 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    53461 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    53521 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    53581 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    53641 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    53701 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    53761 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    53821 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    53881 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    53941 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    54001 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    54061 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    54121 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    54181 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    54241 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    54301 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    54361 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    54421 tcaactgtcc ataaagttta gtctcaacta ttttcatctt cctcactctt tcattccaga
    54481 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    54541 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    54601 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    54661 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    54721 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    54781 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    54841 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    54901 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    54961 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    55021 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    55081 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    55141 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    55201 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    55261 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    55321 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    55381 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    55441 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    55501 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    55561 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    55621 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    55681 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    55741 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    55801 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    55861 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    55921 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    55981 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    56041 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    56101 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    56161 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    56221 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    56281 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    56341 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    56401 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    56461 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    56521 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    56581 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    56641 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    56701 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    56761 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    56821 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    56881 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    56941 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    57001 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    57061 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    57121 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    57181 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    57241 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    57301 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    57361 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    57421 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    57481 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    57541 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    57601 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    57661 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    57721 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    57781 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    57841 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    57901 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    57961 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    58021 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    58081 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacattt
    58141 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    58201 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    58261 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    58321 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    58381 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    58441 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    58501 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    58561 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    58621 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    58681 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    58741 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    58801 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    58861 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    58921 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    58981 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    59041 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    59101 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    59161 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    59221 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    59281 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    59341 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    59401 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    59461 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    59521 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    59581 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc
    59641 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    59701 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    59761 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    59821 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    59881 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    59941 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    60001 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    60061 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    60121 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    60181 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    60241 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    60301 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    60361 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    60421 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    60481 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    60541 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    60601 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    60661 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    60721 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    60781 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    60841 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    60901 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    60961 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    61021 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    61081 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    61141 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    61201 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    61261 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    61321 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    61381 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    61441 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    61501 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    61561 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    61621 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    61681 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    61741 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    61801 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    61861 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    61921 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    61981 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    62041 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    62101 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    62161 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    62221 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    62281 taattggcag tgcgctaaag gcccttccac ttggattaat ggttcatttg caaaaactag
    62341 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    62401 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    62461 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    62521 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    62581 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    62641 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    62701 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    62761 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    62821 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    62881 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    62941 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    63001 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    63061 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    63121 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    63181 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    63241 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    63301 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    63361 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    63421 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    63481 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    63541 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    63601 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    63661 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    63721 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    63781 ttaatagtat aacataattt cgtgagaaaa aaatacaatt ttttcttgac catctcgtta
    63841 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    63901 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    63961 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    64021 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    64081 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    64141 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    64201 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    64261 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    64321 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    64381 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    64441 agcttagcag accgtatctt ccaagctcat aagaagtggt tctcttacag ctggcctgaa
    64501 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    64561 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    64621 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    64681 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    64741 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    64801 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    64861 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    64921 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    64981 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    65041 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    65101 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    65161 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    65221 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    65281 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    65341 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttatgat
    65401 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    65461 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    65521 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    65581 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    65641 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    65701 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    65761 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    65821 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    65881 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    65941 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    66001 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    66061 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    66121 gatactgtaa atatatcttt attattagtg aatg
//
LOCUS       DAYYGY010000006        65201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000006 DAYYGY010000000
VERSION     DAYYGY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.006"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHA52_000390"
     CDS             476..1339
                     /locus_tag="ACHA52_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8939146.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHA52_000391"
     CDS             complement(1468..3090)
                     /locus_tag="ACHA52_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8939147.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHA52_000392"
     CDS             3339..3860
                     /locus_tag="ACHA52_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8939148.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA52_000393"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA52_000393"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8939149.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHA52_000394"
     CDS             5941..6759
                     /locus_tag="ACHA52_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8939150.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHA52_000395"
     CDS             complement(6862..7059)
                     /locus_tag="ACHA52_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939151.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHA52_000396"
     CDS             7228..7806
                     /locus_tag="ACHA52_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8939152.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHA52_000397"
     CDS             7901..8038
                     /locus_tag="ACHA52_000397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939153.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACHA52_000398"
     CDS             complement(8851..9354)
                     /locus_tag="ACHA52_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8939154.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACHA52_000399"
     CDS             9713..10738
                     /locus_tag="ACHA52_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8939155.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACHA52_000400"
                     /pseudo
     CDS             11061..11939
                     /locus_tag="ACHA52_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            12415..12531
                     /locus_tag="ACHA52_000401"
     CDS             12415..12531
                     /locus_tag="ACHA52_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8939156.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACHA52_000402"
     CDS             13459..13797
                     /locus_tag="ACHA52_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939157.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACHA52_000403"
     CDS             13891..14121
                     /locus_tag="ACHA52_000403"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8939158.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACHA52_000404"
     CDS             14521..14892
                     /locus_tag="ACHA52_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8939159.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACHA52_000405"
     CDS             complement(15166..15858)
                     /locus_tag="ACHA52_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8939160.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACHA52_000406"
     CDS             complement(15861..16646)
                     /locus_tag="ACHA52_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8939161.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACHA52_000407"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACHA52_000407"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8939162.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACHA52_000408"
     CDS             18308..18625
                     /locus_tag="ACHA52_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8939163.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACHA52_000409"
     CDS             18638..20311
                     /locus_tag="ACHA52_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8939164.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACHA52_000410"
     CDS             20325..21956
                     /locus_tag="ACHA52_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8939165.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACHA52_000411"
     CDS             complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACHA52_000411"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8939166.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACHA52_000412"
     CDS             complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACHA52_000412"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8939167.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25190..25795
                     /locus_tag="ACHA52_000413"
     CDS             25190..25795
                     /locus_tag="ACHA52_000413"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8939168.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26103..27119
                     /locus_tag="ACHA52_000414"
     CDS             26103..27119
                     /locus_tag="ACHA52_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8939169.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27260..28561
                     /locus_tag="ACHA52_000415"
     CDS             27260..28561
                     /locus_tag="ACHA52_000415"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8939170.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28607..29269
                     /gene="deoC"
                     /locus_tag="ACHA52_000416"
     CDS             28607..29269
                     /gene="deoC"
                     /locus_tag="ACHA52_000416"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8939171.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29290..29688
                     /locus_tag="ACHA52_000417"
     CDS             29290..29688
                     /locus_tag="ACHA52_000417"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8939172.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29754..30836
                     /locus_tag="ACHA52_000418"
     CDS             29754..30836
                     /locus_tag="ACHA52_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8939173.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31048..32610
                     /locus_tag="ACHA52_000419"
     CDS             31048..32610
                     /locus_tag="ACHA52_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939174.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32603..33736
                     /locus_tag="ACHA52_000420"
     CDS             32603..33736
                     /locus_tag="ACHA52_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939175.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33736..34692
                     /locus_tag="ACHA52_000421"
     CDS             33736..34692
                     /locus_tag="ACHA52_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939176.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34791..35957
                     /gene="deoB"
                     /locus_tag="ACHA52_000422"
     CDS             34791..35957
                     /gene="deoB"
                     /locus_tag="ACHA52_000422"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8939177.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35981..36799
                     /locus_tag="ACHA52_000423"
     CDS             35981..36799
                     /locus_tag="ACHA52_000423"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8939178.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36816..37523
                     /gene="deoD"
                     /locus_tag="ACHA52_000424"
     CDS             36816..37523
                     /gene="deoD"
                     /locus_tag="ACHA52_000424"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8939179.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACHA52_000425"
     CDS             complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACHA52_000425"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8939180.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38679..39260)
                     /locus_tag="ACHA52_000426"
     CDS             complement(38679..39260)
                     /locus_tag="ACHA52_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8939181.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACHA52_000427"
     CDS             complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACHA52_000427"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8939182.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40395..40808
                     /gene="rpsL"
                     /locus_tag="ACHA52_000428"
     CDS             40395..40808
                     /gene="rpsL"
                     /locus_tag="ACHA52_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8939183.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40892..41362
                     /gene="rpsG"
                     /locus_tag="ACHA52_000429"
     CDS             40892..41362
                     /gene="rpsG"
                     /locus_tag="ACHA52_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8939184.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41456..43543
                     /gene="fusA"
                     /locus_tag="ACHA52_000430"
     CDS             41456..43543
                     /gene="fusA"
                     /locus_tag="ACHA52_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8939185.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43715..44902
                     /gene="tuf"
                     /locus_tag="ACHA52_000431"
     CDS             43715..44902
                     /gene="tuf"
                     /locus_tag="ACHA52_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8939186.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACHA52_000432"
     CDS             45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACHA52_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8939187.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45648..46277
                     /gene="rplC"
                     /locus_tag="ACHA52_000433"
     CDS             45648..46277
                     /gene="rplC"
                     /locus_tag="ACHA52_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8939188.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46305..46928
                     /gene="rplD"
                     /locus_tag="ACHA52_000434"
     CDS             46305..46928
                     /gene="rplD"
                     /locus_tag="ACHA52_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8939189.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46928..47218
                     /gene="rplW"
                     /locus_tag="ACHA52_000435"
     CDS             46928..47218
                     /gene="rplW"
                     /locus_tag="ACHA52_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8939190.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47257..48090
                     /gene="rplB"
                     /locus_tag="ACHA52_000436"
     CDS             47257..48090
                     /gene="rplB"
                     /locus_tag="ACHA52_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8939191.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48133..48411
                     /gene="rpsS"
                     /locus_tag="ACHA52_000437"
     CDS             48133..48411
                     /gene="rpsS"
                     /locus_tag="ACHA52_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8939192.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48434..48781
                     /gene="rplV"
                     /locus_tag="ACHA52_000438"
     CDS             48434..48781
                     /gene="rplV"
                     /locus_tag="ACHA52_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8939193.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48795..49451
                     /gene="rpsC"
                     /locus_tag="ACHA52_000439"
     CDS             48795..49451
                     /gene="rpsC"
                     /locus_tag="ACHA52_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8939194.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49454..49888
                     /gene="rplP"
                     /locus_tag="ACHA52_000440"
     CDS             49454..49888
                     /gene="rplP"
                     /locus_tag="ACHA52_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8939195.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49878..50066
                     /gene="rpmC"
                     /locus_tag="ACHA52_000441"
     CDS             49878..50066
                     /gene="rpmC"
                     /locus_tag="ACHA52_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8939196.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACHA52_000442"
     CDS             50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACHA52_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8939197.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50415..50783
                     /gene="rplN"
                     /locus_tag="ACHA52_000443"
     CDS             50415..50783
                     /gene="rplN"
                     /locus_tag="ACHA52_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8939198.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50820..51128
                     /gene="rplX"
                     /locus_tag="ACHA52_000444"
     CDS             50820..51128
                     /gene="rplX"
                     /locus_tag="ACHA52_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8939199.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51156..51695
                     /gene="rplE"
                     /locus_tag="ACHA52_000445"
     CDS             51156..51695
                     /gene="rplE"
                     /locus_tag="ACHA52_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8939200.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51714..51899
                     /locus_tag="ACHA52_000446"
     CDS             51714..51899
                     /locus_tag="ACHA52_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8939201.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51935..52333
                     /gene="rpsH"
                     /locus_tag="ACHA52_000447"
     CDS             51935..52333
                     /gene="rpsH"
                     /locus_tag="ACHA52_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8939202.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52365..52901
                     /gene="rplF"
                     /locus_tag="ACHA52_000448"
     CDS             52365..52901
                     /gene="rplF"
                     /locus_tag="ACHA52_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8939203.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53062..53418
                     /gene="rplR"
                     /locus_tag="ACHA52_000449"
     CDS             53062..53418
                     /gene="rplR"
                     /locus_tag="ACHA52_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8939204.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53439..53939
                     /gene="rpsE"
                     /locus_tag="ACHA52_000450"
     CDS             53439..53939
                     /gene="rpsE"
                     /locus_tag="ACHA52_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8939205.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53954..54133
                     /gene="rpmD"
                     /locus_tag="ACHA52_000451"
     CDS             53954..54133
                     /gene="rpmD"
                     /locus_tag="ACHA52_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8939206.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54177..54617
                     /gene="rplO"
                     /locus_tag="ACHA52_000452"
     CDS             54177..54617
                     /gene="rplO"
                     /locus_tag="ACHA52_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8939207.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54617..55912
                     /gene="secY"
                     /locus_tag="ACHA52_000453"
     CDS             54617..55912
                     /gene="secY"
                     /locus_tag="ACHA52_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8939208.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55974..56621
                     /locus_tag="ACHA52_000454"
     CDS             55974..56621
                     /locus_tag="ACHA52_000454"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8939209.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56812..57030
                     /gene="infA"
                     /locus_tag="ACHA52_000455"
     CDS             56812..57030
                     /gene="infA"
                     /locus_tag="ACHA52_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8939210.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACHA52_000456"
     CDS             57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACHA52_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8939211.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57197..57562
                     /gene="rpsM"
                     /locus_tag="ACHA52_000457"
     CDS             57197..57562
                     /gene="rpsM"
                     /locus_tag="ACHA52_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8939212.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57590..57979
                     /gene="rpsK"
                     /locus_tag="ACHA52_000458"
     CDS             57590..57979
                     /gene="rpsK"
                     /locus_tag="ACHA52_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8939213.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58059..58997
                     /locus_tag="ACHA52_000459"
     CDS             58059..58997
                     /locus_tag="ACHA52_000459"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8939214.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59025..59405
                     /gene="rplQ"
                     /locus_tag="ACHA52_000460"
     CDS             59025..59405
                     /gene="rplQ"
                     /locus_tag="ACHA52_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8939215.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59677..60516
                     /locus_tag="ACHA52_000461"
     CDS             59677..60516
                     /locus_tag="ACHA52_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8939216.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60492..61361
                     /locus_tag="ACHA52_000462"
     CDS             60492..61361
                     /locus_tag="ACHA52_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8939217.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61351..62148
                     /locus_tag="ACHA52_000463"
     CDS             61351..62148
                     /locus_tag="ACHA52_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8939218.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62351..63097
                     /gene="truA"
                     /locus_tag="ACHA52_000464"
     CDS             62351..63097
                     /gene="truA"
                     /locus_tag="ACHA52_000464"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8939219.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            63761..63955
                     /locus_tag="ACHA52_000465"
                     /pseudo
     CDS             63761..63955
                     /locus_tag="ACHA52_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(64143..64919)
                     /locus_tag="ACHA52_000466"
     CDS             complement(64143..64919)
                     /locus_tag="ACHA52_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8939220.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            65042..>65201
                     /locus_tag="ACHA52_000467"
     CDS             65042..>65201
                     /locus_tag="ACHA52_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8939221.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTV"
BASE COUNT        20682 a        11649 c        13780 g        19090 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatagttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    22501 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    22561 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    22621 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    22681 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    22741 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    22801 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    22861 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    22921 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    22981 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    23041 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    23101 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    23161 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    23221 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    23281 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    23341 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    23401 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    23461 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    23521 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    23581 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    23641 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    23701 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    23761 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    23821 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    23881 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    23941 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    24001 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    24061 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    24121 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    24181 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    24241 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    24301 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    24361 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    24421 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    24481 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    24541 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    24601 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    24661 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    24721 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    24781 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    24841 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    24901 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    24961 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    25021 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    25081 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    25141 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    25201 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    25261 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    25321 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    25381 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    25441 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    25501 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    25561 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    25621 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    25681 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    25741 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    25801 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    25861 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    25921 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    25981 ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    26041 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    26101 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    26161 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    26221 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    26281 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    26341 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    26401 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    26461 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    26521 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    26581 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    26641 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    26701 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    26761 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    26821 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    26881 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    26941 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    27001 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    27061 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    27121 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    27181 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    27241 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    27301 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    27361 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    27421 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    27481 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    27541 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    27601 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    27661 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    27721 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    27781 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    27841 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    27901 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    27961 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    28021 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    28081 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    28141 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    28201 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    28261 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    28321 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    28381 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    28441 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    28501 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    28561 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    28621 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    28681 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    28741 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    28801 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    28861 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    28921 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    28981 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    29041 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    29101 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    29161 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    29221 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    29281 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    29341 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    29401 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    29461 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    29521 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    29581 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    29641 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    29701 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    29761 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    29821 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    29881 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    29941 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    30001 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    30061 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    30121 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    30181 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    30241 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    30301 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    30361 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    30421 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    30481 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    30541 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    30601 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    30661 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    30721 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    30781 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    30841 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    30901 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    30961 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    31021 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    31081 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    31141 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    31201 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    31261 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    31321 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    31381 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    31441 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    31501 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    31561 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    31621 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    31681 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    31741 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    31801 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    31861 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    31921 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    31981 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    32041 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    32101 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    32161 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    32221 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    32281 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    32341 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    32401 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    32461 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    32521 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    32581 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    32641 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    32701 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    32761 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    32821 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    32881 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    32941 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    33001 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    33061 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    33121 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    33181 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    33241 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    33301 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    33361 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    33421 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    33481 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    33541 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    33601 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    33661 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    33721 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    33781 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    33841 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    33901 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    33961 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    34021 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    34081 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    34141 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    34201 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    34261 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    34321 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    34381 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    34441 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    34501 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    34561 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    34621 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    34681 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    34741 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    34801 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    34861 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    34921 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    34981 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    35041 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    35101 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    35161 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    35221 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    35281 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    35341 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    35401 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    35461 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    35521 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    35581 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    35641 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    35701 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    35761 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    35821 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    35881 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    35941 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    36001 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    36061 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    36121 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    36181 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    36241 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    36301 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    36361 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    36421 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    36481 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    36541 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    36601 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    36661 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    36721 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    36781 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    36841 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    36901 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    36961 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    37021 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    37081 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    37141 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    37201 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    37261 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    37321 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    37381 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    37441 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    37501 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    37561 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    37621 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    37681 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    37741 gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag
    37801 tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa gtcatcattt
    37861 aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc ttcgtcagag
    37921 ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc tactaaaaca
    37981 gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa agcgcgttct
    38041 aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat tgcgtaacga
    38101 cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag agtatcgtaa
    38161 gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc
    38221 aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc
    38281 caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg agttaaaaca
    38341 gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc gccttctttt
    38401 gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa ttggttcaat
    38461 gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt tagctctcct
    38521 tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca attttttgga
    38581 agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga gaatataaaa
    38641 aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa ttgctcgatt
    38701 tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc gcattttgaa
    38761 gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt tttgatcgtt
    38821 tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc atctaatcca
    38881 actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc cattaattgg
    38941 aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat caagtgcagc
    39001 ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt gcctaatcga
    39061 tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa
    39121 tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat ttcttgacaa
    39181 ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt
    39241 gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa ctggatataa
    39301 cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt
    39361 ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc gtcaagaatc
    39421 tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa aagcccatgt
    39481 tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg ttcgatgact
    39541 tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc tccgttttcg
    39601 tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag
    39661 ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac taatttagaa
    39721 gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc gaaaagcaaa
    39781 gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc accgtctatt
    39841 gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa tttttccgct
    39901 tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc ttcacgcata
    39961 cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag acctacgatc
    40021 atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa attcatcttt
    40081 gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc gtgatttacg
    40141 gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa ttgcatctct
    40201 tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta caggtttctg
    40261 tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga cattgcattt
    40321 attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa
    40381 ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt aaatcaaagg
    40441 tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag aaaactcaaa
    40501 ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca atgacaccta
    40561 aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag
    40621 ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt gtattattac
    40681 gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc ggtgctttag
    40741 atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact aaaaaaccta
    40801 aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga agtaattact
    40861 atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg
    40921 tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga tcaaccgtgt
    40981 aatggttgac ggaaaacgcg gtattgcagc taacattatc tataatgcct ttgacttgat
    41041 caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga aaaacgttca
    41101 acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag taccagttga
    41161 agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact acgctcgctt
    41221 gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa
    41281 cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg ccaaccgtgc
    41341 gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata agtcgacctc
    41401 agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa ggagaatgac
    41461 cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca tcgatgctgg
    41521 taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata aaattggtga
    41581 aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac gtggtattac
    41641 gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca tcatcgacac
    41701 accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc
    41761 tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag tttggcgtca
    41821 agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc
    41881 tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg ctcacccaat
    41941 ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg taacaatgaa
    42001 ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa ttcctgaaga
    42061 ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag ctgaaactga
    42121 tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg agttaaaaga
    42181 aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg gttcagcatt
    42241 taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc catcaccgtt
    42301 agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag aacgtcctgc
    42361 agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc cattcgtagg
    42421 tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat acgtacaaaa
    42481 cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg ccaactctcg
    42541 ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt tgaaagacac
    42601 aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat ccatggaatt
    42661 cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc aagataaaat
    42721 gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg aaacaaacgt
    42781 ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata tcatcgttga
    42841 ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag tatcttaccg
    42901 tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa
    42961 aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg ctggtttcga
    43021 atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag ctgttgaaac
    43081 tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag tagatatcaa
    43141 agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg ccttccgtgt
    43201 tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa tccttgaacc
    43261 aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta tgggtcacgt
    43321 aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt
    43381 tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc gttctgctac
    43441 tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac caaaatcagt
    43501 acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg aaaccggata
    43561 gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt gtaaaataat
    43621 tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc agacaaataa
    43681 aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat ttgaccgttc
    43741 taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa ctacattaac
    43801 agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg cttacgatca
    43861 aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag ctcacgttga
    43921 gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg cggattacgt
    43981 taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag tttctgctgc
    44041 tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc
    44101 ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat tactagaatt
    44161 agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg acgatgttcc
    44221 tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg aagaaaaaat
    44281 tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg acaacgacaa
    44341 accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta ctgttgctac
    44401 aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc
    44461 tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat tgttagacta
    44521 cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg aagacatcca
    44581 acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat tctctgcaga
    44641 agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca ctaactaccg
    44701 tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac cagaaggaac
    44761 tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc acccaatcgc
    44821 tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt
    44881 cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca tttattcgga
    44941 cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta tttattgcag
    45001 gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa agagtcagta
    45061 ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt
    45121 gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc acagacgtaa
    45181 tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac gctttgccac
    45241 gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata ggcgaaggag
    45301 ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa caccgtattt
    45361 tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg
    45421 gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg actcataaat
    45481 acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc gacattgtga
    45541 acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct ggtgtaaaca
    45601 tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg accaaaggaa
    45661 tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa ttaattccag
    45721 taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg gaaacggatg
    45781 gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca aacaaacctg
    45841 cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa
    45901 atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt ttccaagcag
    45961 gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg gttatcaaac
    46021 gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt cgtcctgggt
    46081 caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg
    46141 gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg
    46201 ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact atcaaatcag
    46261 ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg aatgtagcat
    46321 tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa atcttcggaa
    46381 ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg tcattaagac
    46441 aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc
    46501 gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa tggcgtggag
    46561 gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct aaaaaagttc
    46621 gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa
    46681 tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt cttgctaact
    46741 tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt gctgctttat
    46801 cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt gtgctagacg
    46861 ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt gaggaggtgc
    46921 ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa aatcaatgct
    46981 tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca aaactttagt
    47041 aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt
    47101 acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc
    47161 gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg ctgaataagc
    47221 actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct
    47281 acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac aacatcaaca
    47341 cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa caacggccgc
    47401 atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat cgacttcaaa
    47461 cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc aaaccgttca
    47521 gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt agcacctaaa
    47581 ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa agtcggaaat
    47641 gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga aatgaaacct
    47701 ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa
    47761 ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt agctgcatgc
    47821 cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat cggtaaagct
    47881 ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat gaaccctaat
    47941 gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc accagtttct
    48001 ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa atcagacaaa
    48061 cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc acattttgag
    48121 aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga gcatttaatg
    48181 aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc
    48241 cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga tggacgtaaa
    48301 catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga atttgcacct
    48361 acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta atttgagggg
    48421 aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa aaacagttcg
    48481 cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga
    48541 tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg aaaaagtatt
    48601 gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa acttggtagt
    48661 aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg
    48721 atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat cagaaaaata
    48781 aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta ggcatcatcc
    48841 gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta cacgaagatt
    48901 taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca acagttgaaa
    48961 tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca ggtatggtta
    49021 ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa ttaactggta
    49081 aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca aaattagtag
    49141 gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag
    49201 ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta tctggtcgtc
    49261 taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt cctcttcata
    49321 ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac ggaaaactag
    49381 gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac actgagaaag
    49441 gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa
    49501 aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc
    49561 tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg caatgactcg
    49621 ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat cttatactgc
    49681 caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat gggttgcacc
    49741 agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga
    49801 agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa aacgtgagga
    49861 aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact gccgaaatgc
    49921 ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa ttagcaacag
    49981 gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa
    50041 ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt atgactgaag
    50101 gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa
    50161 ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc atgaactatt
    50221 ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac atcgtgagaa
    50281 ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa gtagtcgaag
    50341 aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca aaatctgaaa
    50401 ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg
    50461 cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact gctaacatcg
    50521 gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc aaaaaaggtg
    50581 acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat
    50641 atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc cctcgtggaa
    50701 cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt
    50761 cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc gaaacagtta
    50821 tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa aacaaagaag
    50881 gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg
    50941 ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt gagatggaag
    51001 cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct ggccgagttg
    51061 gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag
    51121 ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc
    51181 ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt atgcaaacac
    51241 ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca aacgctaaaa
    51301 acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca atgattacaa
    51361 aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag
    51421 ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct gtttcactac
    51481 cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata
    51541 ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta gtagataaag
    51601 tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt
    51661 tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac tacgtggcta
    51721 aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc
    51781 gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt tgccgtattt
    51841 gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa
    51901 aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc caattgcaga
    51961 ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag aagtacctgc
    52021 atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt
    52081 tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa
    52141 cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt
    52201 caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca tttcgacttc
    52261 tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc
    52321 ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa
    52381 ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac aacgttacag
    52441 ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa atgaatatcg
    52501 aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa acaattcatg
    52561 gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag
    52621 cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa cttgtattga
    52681 acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact gttgaagtac
    52741 cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc gaattagctg
    52801 caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt cgttatgttg
    52861 gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat
    52921 agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa aatacatttt
    52981 tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata gtagtacaat
    53041 aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac acgtcaaaag
    53101 agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg cttgaacgtt
    53161 tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg tgtaacgcta
    53221 gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga agcagcacaa
    53281 gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt
    53341 gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa
    53401 aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc gatccaaaac
    53461 atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg
    53521 gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga cacgtagggt
    53581 ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt gaagatgcga
    53641 agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa gtaatcggtg
    53701 cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg gttgctgctg
    53761 gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact tctaaatctt
    53821 taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg aaacaattga
    53881 aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta atcggataag
    53941 gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta tcggacgtcc
    54001 tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca gttcagtggt
    54061 taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc tagtggacgt
    54121 tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc aaacctatga
    54181 aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg
    54241 gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt
    54301 caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc cgtcgtttac
    54361 caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat cttgatgtat
    54421 tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg
    54481 tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac aagaaattaa
    54541 ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg gctggtggat
    54601 caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa aagacattag
    54661 atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg cacatattac
    54721 tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat ttttcaacat
    54781 gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa tgggggtttc
    54841 accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg tgcctcgatt
    54901 tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata
    54961 tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat tcaatcaata
    55021 cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga tcggtttgat
    55081 tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag aaaaaggtat
    55141 cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc ctgaaagtat
    55201 cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat ggaaatctgc
    55261 tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg taacattttt
    55321 ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac
    55381 aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta tctttgctag
    55441 ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat tcggttctga
    55501 aaactggtac aaagtgatga tggagatctt taactataat acagcacctg gtgctacgat
    55561 ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag taaaccctga
    55621 gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac gccctggtaa
    55681 ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg gtgcagtctt
    55741 ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc ttccacaatc
    55801 aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag aaactgcgaa
    55861 acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt
    55921 gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa acaatgaacc
    55981 tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa atcattgatg
    56041 cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg caaaacgaaa
    56101 cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt cctgatgaag
    56161 taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa ggcttcttat
    56221 tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg ctaaaagatt
    56281 tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac
    56341 gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa attttcaacc
    56401 ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat caaagagaag
    56461 atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa
    56521 tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct cgtgaaatcg
    56581 atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc ccgtagccga
    56641 cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata tgtcagtccg
    56701 acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga actgcagttt
    56761 ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa
    56821 gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa
    56881 gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat tcgtatgcat
    56941 tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata cgatcttaac
    57001 cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca ggaggtatta
    57061 tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta
    57121 aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt caaggataat
    57181 tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg ataaacgtgt
    57241 agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa agatcttagc
    57301 tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc
    57361 tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt
    57421 gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc gccgtggatt
    57481 gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc
    57541 tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca tggcagcaaa
    57601 aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg cacatatcca
    57661 ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg cattagcgtg
    57721 gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca
    57781 aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga ctgttgaagt
    57841 aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac aagctacagg
    57901 tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg cccgccctcc
    57961 aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta acgtcattga
    58021 acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc gaaaaaccaa
    58081 gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa
    58141 gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct tctttgccag
    58201 gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct accatcaaag
    58261 gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg ctgaaaatgt
    58321 atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca gtaactgctg
    58381 gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac atctgtagtg
    58441 tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt ggttatgttc
    58501 aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc gattcaatct
    58561 acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga caccgtgaag
    58621 attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg ttggaagcaa
    58681 tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac ttaactgatg
    58741 aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg
    58801 aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg aaacgtgcag
    58861 gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc aaagtccgta
    58921 acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta ggtttaggtt
    58981 tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt tatcgtaaac
    59041 taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact gatttattaa
    59101 tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca actgcagaaa
    59161 aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg
    59221 tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt
    59281 tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc ggatacacac
    59341 gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc ttagaacttg
    59401 tctaaaatca cactaataac atcactttat agatgattct tttagagtgt tatgatgttg
    59461 gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg cttggggagt
    59521 aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac agatatgttt
    59581 gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt tttttgatag
    59641 tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat tgaattagaa
    59701 aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga tgtctccttt
    59761 acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg aaaatcgaca
    59821 cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa agtgggaaat
    59881 caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat ggtttttcaa
    59941 aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt tggtcttgaa
    60001 aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt
    60061 agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca aaaacaacgt
    60121 gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga tgaagcaacc
    60181 agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa
    60241 gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc taatgccaat
    60301 cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa agaaattttt
    60361 tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga aaaactgaag
    60421 agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga agaaaggatg
    60481 gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag ccgaatacac
    60541 catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac agttatactg
    60601 cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg aatgcgcttg
    60661 ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg gatacggata
    60721 ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac
    60781 agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca
    60841 gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg ctggatgaaa
    60901 gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga gtagcaatcg
    60961 ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg gcaggacttg
    61021 atccccaggg tagaaaagaa atgatggaga tgttttggcg tcttcataag gaacagcaga
    61081 tcactattgt attagttacg catttaatgg atgatgtagc caattttgca gattatgtct
    61141 atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc tttcaaaaca
    61201 ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt gcagaagaat
    61261 taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa ttagcagacg
    61321 ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt tggtcgttat
    61381 atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt agccagtttt
    61441 tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt gttggcattt
    61501 ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat ccgaggcatt
    61561 cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt tacaagtggc
    61621 ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt gatgaacggc
    61681 ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact cacgcttact
    61741 acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta
    61801 cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca
    61861 ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt
    61921 gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc tttatttgtc
    61981 agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg ctacaaaggt
    62041 ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac gatggttatc
    62101 attgcttttg tattattaac tgtgctactg atctttttga gaggataaac gttcagtggg
    62161 ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcgagc agtgtatgac
    62221 aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt atctgaaaag
    62281 aaagaacgaa aaaattttat aaaaaaatca ttattccaga taacctttta ggaacaaagg
    62341 agagaaaaac atggtacgtt acaaagcaat cattgcatac gacggaacaa attttaatgg
    62401 attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa
    62461 aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg ccggagtaca
    62521 tgcgattggg caagtaatcc attttgatta tccagaggag cgtccgttag aacgaatgcg
    62581 tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag aaattgtttc
    62641 agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc gagtggatat
    62701 agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt accctcttga
    62761 tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt tcacctcttt
    62821 ttgtgcatca ggaagctcga tagaggataa agtacgtacg atctacgagg ctaagatgga
    62881 ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct tcttgtataa
    62941 gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat tgcctgaaga
    63001 tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta cagcacatcc
    63061 tgaaggactt tatttgtatg aagtgaaata tgaataagag gttgtgacag aagtggtcag
    63121 cttcaagaaa taagaaggaa tttacgaaaa ttacttctca aaattttgca aaatttcggc
    63181 ttattttcga aggagctact tctggaacac cgtgaatcag aggttgtgaa aaagctgccc
    63241 tgcatcaagt aataagaaca accaaacaaa aatagctcgt catatttttc gtttggttgg
    63301 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    63361 gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
    63421 ttgagctaat accgaaggtc agctgattga acaccgttta agaaagaggt cgtgagcctg
    63481 tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca gcttctcatc tttgatgaat
    63541 atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct
    63601 gtaaccaaaa aacgatggaa cgttgttttg attgccctta atgaggacag tcggaacagg
    63661 tttgtcattt ttatatttaa atggcacaaa tttttctaat tatttgtcat ttagcgctga
    63721 aaatttttgc tatttatcta aaaaagtgat aactacttaa gatgaagcag aatatttacg
    63781 ccatacggca tacaaccgcg aaaaatataa aaaaaagaaa acgattgaac gcgtttttgc
    63841 agtcgcaaag gaaaagcatg gaatgcgcta catcaagtat cgagacttgg aaaacaatca
    63901 ggtgtatacg ctgattgttt ttgctgcaat gaatctgaaa aagttgtcca cttggcagtt
    63961 ggaacatatg tcttcatatc ttttattata caaaaatagc aaaagcacca gtctacactc
    64021 ttagtaaaga ggaaactgat ccttttgtct acattcttaa agtacttaaa ctaagtactt
    64081 tattttactc tttaggattg atacagtagc atctatccct ttattaataa tcttttattt
    64141 ttttaaataa agatgttgtc tccatcttca ggcactaaaa ctttttcgga aattcctttt
    64201 tctttattga aagactttaa ttcttctctt gacagtcccc aatggtttac agcttccata
    64261 tgaacggcaa taattgtgga atctggtatt gctttatgca attcatataa atcgtctttc
    64321 cccataataa gagattctcc aactaaaaaa tgattatctc cagcatttgc tactatgatt
    64381 tctggattat actgatcaat agtttgttta acagcttcat accaaactgt atctcctgta
    64441 aagtatagtg tcttctcagt cgaatgtttg aaaacaatac cgcaaacatt acctacataa
    64501 ttcaagattt ctcctctgcc atgtttgcct tttgttttaa ccaattgtat agattcaaaa
    64561 actgtgtctt ctcgtagtac ctccacattt ttaaaaccat atagtcgaat ttcattcgca
    64621 tcattttcat cttgcacaaa taattttatt tctttaggta ggacctcttt cgctatatcg
    64681 tcccaatgat ctagatgtaa atgtgttaca ataacagcat caataccgtt gataatatct
    64741 tcgactggca ttggcaaatc aactagagga ttcatttgat cttttcttgg tgatggaaaa
    64801 ggacctttcg aaataaatgg tggaagtgtt cctttttccg ctaacattgg atcaattaaa
    64861 aattttttac ctccatagtc taagatagct gttgcattac gaatgtgatg tatattcata
    64921 aacgttgtct cctttttgtt gatatgaaat atttttagta taattcaatt ccatacaata
    64981 aataaaccga acaaaaaaag tcttttacta tttttactta atttatgaaa ggttgtttac
    65041 catgttagac caaacagata taaatatcat tgaggaacta tcaaaaaata gtcgaatcac
    65101 aatgaaagaa cttggagaaa aagtacattt gactggaccc gctgtctcgg cgagagtgac
    65161 aaaactagag gaaagtggag ttattgaaaa ttatacagta a
//
LOCUS       DAYYGY010000007        57482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000007 DAYYGY010000000
VERSION     DAYYGY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.007"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..716
                     /locus_tag="ACHA52_000468"
     CDS             384..716
                     /locus_tag="ACHA52_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8939222.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            626..820
                     /locus_tag="ACHA52_000469"
     CDS             626..820
                     /locus_tag="ACHA52_000469"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8939223.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            859..1005
                     /locus_tag="ACHA52_000470"
     CDS             859..1005
                     /locus_tag="ACHA52_000470"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939224.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1443..2783
                     /locus_tag="ACHA52_000471"
                     /pseudo
     CDS             1443..2783
                     /locus_tag="ACHA52_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3024..4640)
                     /locus_tag="ACHA52_000472"
     CDS             complement(3024..4640)
                     /locus_tag="ACHA52_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8939225.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4758..4976
                     /locus_tag="ACHA52_000473"
     CDS             4758..4976
                     /locus_tag="ACHA52_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939226.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5171..5632
                     /locus_tag="ACHA52_000474"
     CDS             5171..5632
                     /locus_tag="ACHA52_000474"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8939227.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5825..6064)
                     /locus_tag="ACHA52_000475"
     CDS             complement(5825..6064)
                     /locus_tag="ACHA52_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939228.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6088..7611)
                     /locus_tag="ACHA52_000476"
     CDS             complement(6088..7611)
                     /locus_tag="ACHA52_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8939229.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7629..7751)
                     /locus_tag="ACHA52_000477"
     CDS             complement(7629..7751)
                     /locus_tag="ACHA52_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8939230.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7781..8764)
                     /locus_tag="ACHA52_000478"
     CDS             complement(7781..8764)
                     /locus_tag="ACHA52_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8939231.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8788..8937)
                     /gene="rpmG"
                     /locus_tag="ACHA52_000479"
     CDS             complement(8788..8937)
                     /gene="rpmG"
                     /locus_tag="ACHA52_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8939232.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8958..9335)
                     /locus_tag="ACHA52_000480"
     CDS             complement(8958..9335)
                     /locus_tag="ACHA52_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939233.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9367..9546)
                     /gene="rpmF"
                     /locus_tag="ACHA52_000481"
     CDS             complement(9367..9546)
                     /gene="rpmF"
                     /locus_tag="ACHA52_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8939234.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9561..9830)
                     /gene="rpsN"
                     /locus_tag="ACHA52_000482"
     CDS             complement(9561..9830)
                     /gene="rpsN"
                     /locus_tag="ACHA52_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8939235.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10353..12119)
                     /locus_tag="ACHA52_000483"
     CDS             complement(10353..12119)
                     /locus_tag="ACHA52_000483"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939236.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12079..13833)
                     /locus_tag="ACHA52_000484"
     CDS             complement(12079..13833)
                     /locus_tag="ACHA52_000484"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939237.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13943..14512)
                     /locus_tag="ACHA52_000485"
     CDS             complement(13943..14512)
                     /locus_tag="ACHA52_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8939238.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(14530..15945)
                     /locus_tag="ACHA52_000486"
     CDS             complement(14530..15945)
                     /locus_tag="ACHA52_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939239.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(15956..16624)
                     /locus_tag="ACHA52_000487"
     CDS             complement(15956..16624)
                     /locus_tag="ACHA52_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8939240.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(16754..17338)
                     /locus_tag="ACHA52_000488"
     CDS             complement(16754..17338)
                     /locus_tag="ACHA52_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8939241.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(17669..17902)
                     /locus_tag="ACHA52_000489"
     CDS             complement(17669..17902)
                     /locus_tag="ACHA52_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8939242.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(17917..18867)
                     /locus_tag="ACHA52_000490"
     CDS             complement(17917..18867)
                     /locus_tag="ACHA52_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8939243.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(18864..19706)
                     /locus_tag="ACHA52_000491"
     CDS             complement(18864..19706)
                     /locus_tag="ACHA52_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8939244.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(19708..20427)
                     /locus_tag="ACHA52_000492"
     CDS             complement(19708..20427)
                     /locus_tag="ACHA52_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8939245.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            20975..21277
                     /locus_tag="ACHA52_000493"
     CDS             20975..21277
                     /locus_tag="ACHA52_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939246.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            21349..22575
                     /locus_tag="ACHA52_000494"
     CDS             21349..22575
                     /locus_tag="ACHA52_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8939247.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(22663..23055)
                     /gene="rpsI"
                     /locus_tag="ACHA52_000495"
     CDS             complement(22663..23055)
                     /gene="rpsI"
                     /locus_tag="ACHA52_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8939248.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(23071..23499)
                     /gene="rplM"
                     /locus_tag="ACHA52_000496"
     CDS             complement(23071..23499)
                     /gene="rplM"
                     /locus_tag="ACHA52_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8939249.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(24033..24500)
                     /locus_tag="ACHA52_000497"
     CDS             complement(24033..24500)
                     /locus_tag="ACHA52_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8939250.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(24620..26158)
                     /locus_tag="ACHA52_000498"
     CDS             complement(24620..26158)
                     /locus_tag="ACHA52_000498"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8939251.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            26238..26924
                     /locus_tag="ACHA52_000499"
     CDS             26238..26924
                     /locus_tag="ACHA52_000499"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8939252.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(27125..27652)
                     /gene="msrA"
                     /locus_tag="ACHA52_000500"
     CDS             complement(27125..27652)
                     /gene="msrA"
                     /locus_tag="ACHA52_000500"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8939253.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(27736..28413)
                     /gene="pgmB"
                     /locus_tag="ACHA52_000501"
     CDS             complement(27736..28413)
                     /gene="pgmB"
                     /locus_tag="ACHA52_000501"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8939254.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(28410..29540)
                     /locus_tag="ACHA52_000502"
     CDS             complement(28410..29540)
                     /locus_tag="ACHA52_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8939255.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(29540..31723)
                     /locus_tag="ACHA52_000503"
     CDS             complement(29540..31723)
                     /locus_tag="ACHA52_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8939256.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(31720..32745)
                     /locus_tag="ACHA52_000504"
     CDS             complement(31720..32745)
                     /locus_tag="ACHA52_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8939257.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(32790..33617)
                     /locus_tag="ACHA52_000505"
     CDS             complement(32790..33617)
                     /locus_tag="ACHA52_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8939258.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(33614..34681)
                     /locus_tag="ACHA52_000506"
     CDS             complement(33614..34681)
                     /locus_tag="ACHA52_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8939259.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(34707..35543)
                     /locus_tag="ACHA52_000507"
     CDS             complement(34707..35543)
                     /locus_tag="ACHA52_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8939260.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(35540..36409)
                     /locus_tag="ACHA52_000508"
     CDS             complement(35540..36409)
                     /locus_tag="ACHA52_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8939261.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(36479..37762)
                     /locus_tag="ACHA52_000509"
     CDS             complement(36479..37762)
                     /locus_tag="ACHA52_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8939262.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(37776..39461)
                     /locus_tag="ACHA52_000510"
     CDS             complement(37776..39461)
                     /locus_tag="ACHA52_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8939263.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            39736..40761
                     /locus_tag="ACHA52_000511"
     CDS             39736..40761
                     /locus_tag="ACHA52_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8939264.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(41379..42767)
                     /gene="ltrA"
                     /locus_tag="ACHA52_000512"
     CDS             complement(41379..42767)
                     /gene="ltrA"
                     /locus_tag="ACHA52_000512"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8939265.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(43255..46908)
                     /gene="rpoC"
                     /locus_tag="ACHA52_000513"
     CDS             complement(43255..46908)
                     /gene="rpoC"
                     /locus_tag="ACHA52_000513"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8939266.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(46987..50604)
                     /gene="rpoB"
                     /locus_tag="ACHA52_000514"
     CDS             complement(46987..50604)
                     /gene="rpoB"
                     /locus_tag="ACHA52_000514"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8939267.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            51332..52300
                     /locus_tag="ACHA52_000515"
     CDS             51332..52300
                     /locus_tag="ACHA52_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8939268.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            52318..53223
                     /locus_tag="ACHA52_000516"
     CDS             52318..53223
                     /locus_tag="ACHA52_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939269.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            53220..54026
                     /locus_tag="ACHA52_000517"
     CDS             53220..54026
                     /locus_tag="ACHA52_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939270.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            54183..55151
                     /locus_tag="ACHA52_000518"
     CDS             54183..55151
                     /locus_tag="ACHA52_000518"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8939271.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(55468..55992)
                     /locus_tag="ACHA52_000519"
     CDS             complement(55468..55992)
                     /locus_tag="ACHA52_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8939272.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            56279..>57482
                     /gene="scm"
                     /locus_tag="ACHA52_000520"
     CDS             56279..>57482
                     /gene="scm"
                     /locus_tag="ACHA52_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8939273.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDT"
BASE COUNT        17156 a        11767 c         9850 g        18709 t
ORIGIN      
        1 aacgaatgat tttaattatc aacgtttttt aattcgtttt tagcttaaaa ataacttttg
       61 tcacagtctc actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc
      121 agaactgggc tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
      181 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
      241 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
      301 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
      361 gttaccggaa ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga
      421 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
      481 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
      541 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
      601 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
      661 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
      721 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
      781 tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac
      841 caaattgatg ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc
      901 tatttgtcca cctatattga cttagcaact cgtattctac gttactatat ggtagattca
      961 cacatgaaaa aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg
     1021 gtaattcatt ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac
     1081 gaccaccaca tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt
     1141 gaagcgtttc atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa
     1201 atagagatga aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc
     1261 catacaaaat ttatgatgac tccttgacaa tttgaagcga agaaaataag ggatctataa
     1321 tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta ataaacgcaa
     1381 acagacacct taaaatcctt tagaataaag tcgacgaaaa ccattcaaag gagtgtttca
     1441 agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta aatcttatct
     1501 ttcatgaaga tgcctttcgc aaagagcgaa tcaatgacga aacatgtttt gtttttgatg
     1561 gcacattgac ctatcaaccg gaggaatgct ttcatcgcca ctatcaaaat gaacaaacga
     1621 tcattaaata gggctggaaa aaagtatcca tcttattgaa tgatgtcagt aactacaaga
     1681 ccattctgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag acttttttag
     1741 cagaagattc agttagtgat cgtcgctgct ccattgctcg cagagtcaaa caagccattc
     1801 ttgagctatt aagcgaacct ctttctatgt ctttgatcgc acgtatgaag cacatttctc
     1861 cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccatttct ttgagccagc
     1921 tgttaccaga agtggtgtgt ttcgatgaat tcaaatcagt gaaaaatgtc tcgggagcca
     1981 tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg gaaaatcggc
     2041 aactcaatcc tttacgcgac tatttcttgc gctaccctat aaaagtccgt gaacaagtgc
     2101 gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag tgttttccga
     2161 atgcccgaat cgtcgccgat cgctttcata ttagccaaca cattggtcgg gcttttacca
     2221 accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg aagcacttaa
     2281 agaaatactg gaaactactt caaaagaatg cgtgggaatt gaaaggtcag catcgctact
     2341 ggcgtccgtc atttcgagat catctgacag aagcagagat agtcgatcgg ctattatctt
     2401 atgatgattc cttaaagcga ggctacgaag tctaccagga ttttctttcc gttatcagaa
     2461 gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag ttgccagagc
     2521 actaccaacc agtgttcacg accttcaaga aataccaaac agaaatcaaa cgagcgttgc
     2581 gcgttcccta ttccaacggc cctatagaat gtttgaataa ccatatcaaa gtactaaaac
     2641 gaattgccta tggctataga aactttcaaa actaccgaga acgaatcttt ttatatcgag
     2701 gaaaatattt taagaaaact aagaatacta cccaactgac taaagccaga acaacaacac
     2761 ggctagacaa gatagctgtc tagccgtgtg agtatctcga ttattctatt gaatttcttc
     2821 accaacactc attgacgaag agccaaaata agtaatctaa ccgactgtta actaaaccat
     2881 tgcaggtccc gttaaagata cttgtgcctg ttgtcaccgc ttctttttct tgtccacaag
     2941 cgcttaacac acttaataaa gcccctgcta ttattaataa taatatcttt ttcttattaa
     3001 aaacctcctc atctatttcc agattagtaa atgtagtaat ctgtgctttc ttttttctca
     3061 atactaataa ctaacttatg gggtaaacaa atactcgttt gaccgggttt tgagatccat
     3121 ccagttctaa ccgcgatttg atcaggacta ttatcttctt taactcgaat tcttccatct
     3181 ttaatctcaa caatattata ttgtcccttc gcaggatgat aattcacgag tttatgattg
     3241 atcttatcca aatcgaagcg atccatttcc ttgccatcaa ttcgaacgat ggcatatttg
     3301 atagcatctt cagttgtttc attctccttt tctatagaga atacaaataa gggaataaaa
     3361 gaggtaataa tcaaaatgcc aataattatg acatcgccta tcttgacacc agcttttctt
     3421 aaaaatttca ttaacgatat ctggctaaac atattagact ttttccatgg aaatggtgaa
     3481 atttttcgga aattatctgc attaagagaa tcagttaaac aaccctcaag ttgttcctta
     3541 atacattgtt gatccatctt cataccaata aaaactagtt tattaatgcg ttcaccaaac
     3601 aacggatgcc aggcttcctt caattcggga tttttttgta taatcatttg acactcaatt
     3661 ttaggtaaac tagctaccca ataatttaat gattcaatac ttttatagct acccgatgtt
     3721 tcaaagatcg tcgaaaaatc actatgttgg gcaaaccaaa gaaacccttt tgctcttgta
     3781 atattgccag gaaaattttt atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac
     3841 cgatttcgat agataaaact tgagatacca tattcttctg tttcaggaat atgattttga
     3901 tagccaagct ctaactcttt gaaccagccc actgaatagg aagtctcatc tatattaaaa
     3961 agatttgtat ttagaatttt cgataagtct acttcacctt taattgtcct aattattttg
     4021 gcattggact gtaacttagc aattagaccg ttaattttgt aaatcgtctt ggttgacact
     4081 aagtcactat tatttaaaag taatatgtta caacactcga attgatcaat taacaagcca
     4141 cgaatatcct gttcttcttc aactacatta ccttcagctt ttctttcctg aatcttttca
     4201 cccgtttcga aattatccca aaaacgtttt acatccacta ctataataat ggcaccaatc
     4261 tctaatcgtt gggtcaaatc aatgtctaaa gggtttttag ctaatataat tgcttgaata
     4321 attggaattg gtttgctgat tcctgaagct tcaataacaa tttgattcaa gtcagaaaat
     4381 tgcactaatt gatctagttc aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca
     4441 ttcggcatcg aaacaagttt ttctccagtt cttttaaaac ctttcttgtc gactaatacc
     4501 ccatcaacat ttacttcccc tatgtcattt acaatgacag caattttctt tccgtgatca
     4561 ccgtacagaa tatgatttag aagtatcgtt tttcctgaac caagatagcc agttaagatt
     4621 gtaataggta tttttttcat agggaacctc tttctatacc ttgaatttac actttaagtg
     4681 caacacataa aaatgagtca taatcgatat ccaactagta taagtttgtc gagaacactc
     4741 ctagaaggaa aataattatg agccagtatc gtcatcttac tatactcgaa cgagaaagaa
     4801 tctttctgct tcacgaagaa ggattttcta ttagtataat tggcaacgac attcagcata
     4861 gcccgtttac tattttacat gaactgagaa gaaacaagaa aaaaactatt caccttccaa
     4921 agcggaaaaa aattatgctt atcgaagaaa aaaatgcgga cgttatcaca ttctaaatga
     4981 tccgtgagtt tggtcaattg ttcgtcattt atttgtcgat tttcagtggt cgccagagga
     5041 aatctcgaat cgtttgaagc atgaacatat ggacttttca attagtttta tcacgatcta
     5101 acgtgcaatt tgcaatgatc ttttcgatac agaacccctc tcacacggca ataaaggcct
     5161 gatccgttca ttgcaccatc gaggaaaaac aagacataca aaaaatcatg tcgagtctcg
     5221 tggaaagatt tgccttactc attcgatcca tggccgtcca aaatccgcag atgatagaag
     5281 tgattttggt cattggaaag ccgatattgt agctggcaaa actggtggta cttgtttagt
     5341 gactttgaca gacagaaaat catgcttcct tttagcagga aagaaggtcc ccagaaagaa
     5401 agccgcttat gtgagcgaga aaatgattga acttttaagc agtattccaa atgaggcggt
     5461 caaaacgatc actccagata gaggaaaaga attctccaat catttcgcag ttacagaagc
     5521 attgaataat atgccctttt attttccaga tcctcatgcc cttggcaaca atgaacaaat
     5581 taaaatacga atagactcat tcgagaatac ttgccaaaat cgaaagagat agctacggta
     5641 gataactcaa cgataaatca gatgattgaa aaactgaata acagacctag aaagtgttta
     5701 gattggagaa caccttatga agtcttcttt gataaagtgt tgcgcttgat ttgacaattc
     5761 aagacataat aaaaagcaga tcaaatccaa ataagggaaa aatgatctac tctggtatta
     5821 tcaattaatt ggccaagcta ttatgccgtt ttgctaaacg tctacttctt ccagtattga
     5881 ccttgataaa acaacaatca ggatcccgtt ttaatactct atgaactgtg tcaatactaa
     5941 catgtaaagt tgcagcaata tcagctaatg tactcccatt taatctcatt tgctttgcaa
     6001 atcgataacg ccgataagca tcaatattct tggaatccat agacaataat acttttctta
     6061 ccatgtattt cctccaaaaa ttgaatttta atgagccatc atttcttgag caatttcatg
     6121 tttagacaat gaagctggat agaaagtagg ccaattatgc aattcattat agagtttttc
     6181 ttgactttct ccaccaaaga aaatatggaa atgctctgcc ttacttggtg caattccatg
     6241 atcactaaat tgaatatatt taaaacgacc agcacctgca tcatcagttt caaaattaaa
     6301 acgaacacca cggtttcctt tttcataatt caaaattttg tatccaacat atttgtatgt
     6361 gtattgatga tgttcaccgt tgactacaaa atcaatggtg ttgtctttaa tgttgatagt
     6421 atcaatatct gtcttatagc cagtagtata ataatctttg tactcggcag ctgtcatgtc
     6481 tttattgatt tttgctttgt aatcgaaaac ttcatccaaa attccatctt ttaacaacgg
     6541 ataaacagat tgccattcgc ctgtataatc actcaattcg cgatccttca catcggcatc
     6601 tttaaagtac ccattagcaa ctgtcttttc atccttcgct tctttttctg gaactacctc
     6661 ttcaccagca accatagttg ttttttctaa cgcttttaga ttgtcttcca ttactgaaac
     6721 ataatcttca ccattatcca tttgttcttt tgtaagactt tcaagaggat tcagtacttc
     6781 caattttatc ccagcttcgt tggccaaagt ttttgcaatt ttatcattag cattcttttc
     6841 aaagtaaata taattgatct tatttttttt aacatactct tttaattcag ctaatcgacc
     6901 agaagatggc tcttcttcag gacttagtcc agcaatagga acttgtttca atccataatc
     6961 caaagctaaa tatccaaagg ctgcgtgttg cgtcacaaaa cttttttgtt tcgcttcttt
     7021 aagactagtg gtatagtcag catccaaccg tttcagttta gtcaaatact tttcagcatt
     7081 tgtctcaaat acttttgctt ttttcggata caatttaact aactgatcct tgatatttga
     7141 aacttcctta attgccattt tgggtgaaac ccacgtatga ggatctagtt catgatggtg
     7201 atcttcttct ccgtgatcat gatcttcttc ttcactacct ggcaataaaa tcatgtcttt
     7261 tgttccttct actacattag gcccagcttt cttccaactt tctttagctt ttggtaccca
     7321 tgattccatg ttttcgttat gatatacaaa aacatcagca tcatgagctt ttgccatatc
     7381 cttagctgag ggctcatagt catgaggttc tgatcccgct ggaaccatta aatccacgtt
     7441 cgcttcatcg ccgacgatgt ttttggtgaa atcatacatt ggatagaatg ttgttacaat
     7501 ttctaatttt ccatctttat cattcgatac ttttgcatca ttagtgctgg agcaagctcc
     7561 caataatcct agagctgcta tcccaataag tcctacactg attttcttca ttctttttcc
     7621 ttccttaatt atgctaattt cttcatttta tttcgtttat gttctttgat aatttttaat
     7681 gccctttttc tagttttaat attcgggctt ttaagtcgtc gatatgcact agatatatcc
     7741 gttttctcca ttagaaatta cccccaatca ctacttatca ttatttgctt tccgccagta
     7801 atttatcaaa actctgtttg atcctttctt catccaacat ttttccgatt agaataatag
     7861 tggtttggtc tttttgtcga gtggtgtatt gaaaattaac ctgttcgttt acctcttgca
     7921 tggcaactat aaaatcctgc tcttgtaaag caatcaatcc tttaaaacga tacaggctcc
     7981 cttggttggt taaaatcagc cagtcaatcc attggagtaa aaaatctttt tttatgtctt
     8041 gatctgaagt taagagaatt gatttaaact catgtacttg atggtgttga tgatagtatt
     8101 tgccattata gtgattttct gatgaatcac tttcaaaaac taagggatga ttaaattttt
     8161 tcaaattata aaaatccttt ttttgaatcg tttcactatg tgtgaacatt cgaatatcgg
     8221 ataaagggtt aatacttttg atttctctct gtaatttttt tagtgcagat aaattcaagt
     8281 tttgttttct agacacaaaa atgcgatctg ccatgacaag ttgcttaagg gcttgtggtt
     8341 ctctttcaat aagttcaaaa ctgtcactat caataacagt caataaacta tcgatatata
     8401 ctcttcctct tagttgaggc gctgttaaaa gtgtttgaat gattggttgg ggatctgcta
     8461 tacctgtagt ttcaatgatg atttgtgaaa ttggttgttg atgttcttct attgtttcca
     8521 gtaaagaaat caatattgct aataaatcac tgcgaagact gcaacacata catccattat
     8581 ttagttgtaa cacccgttcc tcgctatgaa ctaataattt gtgatcaatc ccaacttcac
     8641 caaactcatt ttcaattata ataatttctt ccctggggta gggtgagact tgcaaggctt
     8701 gattgattag cgttgttttt ccagccccta aaaacccaga aacgattgta actggtatgc
     8761 ccattttgtc acctttttac cacttagcta tttcacttct ttaaaaattg cccgtctgcg
     8821 caattttggc gaatactttt ttaattctaa ccgttcaggg ttattccgtt tatttttgct
     8881 ggtgagatac agacgttctc cagtttcgac acattccaaa ataatatttt gacgcataaa
     8941 tcttcctcct tcaatactta tcgatcttca agatatgctt gaatttgttg tgcatcttca
     9001 aaagttaact cgccagattc caattgtgaa actaaatttt ctttcatatc aacaggtatc
     9061 tcccaataac catcgataca ttgaaataac tcctcgtcat actcttcaaa aattggatag
     9121 attcttaaaa aatcatcaaa tgataagcct tggtcctcat agtaatcttt gactaatcca
     9181 ttttcaattg cctgttgggt cggtaaaccc gtttcagtaa taattcctct ctctaccatc
     9241 gaacgataaa aagtgcattt atctagcgat tgctgggtcg tttcaagata atactttaat
     9301 ccttcttcaa ctaaaggtgg gtaaataatt tccattttga ttccctccta aaagttatat
     9361 attaaactat tttgaagctc ctttaattac tttctttcca ttatagtacc cttttaaaga
     9421 tacatgatgg ctacgtctat aatcaccatt cgcttcgtca aatgaaattt ctggttttac
     9481 tacagtttga tgagttcttc ttaaccgttt cttcgcctta gatgtttttc ttgctggtac
     9541 tgccatgata tttcctcctt ttaccaactt gcttttttta ctccaggaat caacccttga
     9601 tgggcaagtt ctctaaactt aatacgggac ataccgaact ttcgcatata ccctcttgga
     9661 cgaccatccg tctgatcacg aagcctcaga cgattcggat ttgaatctct tggcaattta
     9721 gcgagagcgg tgcgatctcc tgcggctttt aattcctgac gtttatctgc atacttttca
     9781 atcatcgcca tttgtttctt tgctttagca attttcgact tttttgccat atacctttat
     9841 actcctttta ttttgattta gacacctcaa atcgtaatcg ttacgttttg acactgatac
     9901 tttaccaaca agagttgctc ttgtcaacaa taaataacat gaaataaaac tcatatgaat
     9961 agataactat ttgaaaacga atttatcggt acatagagat aatcaaatca tttaaaagaa
    10021 tatgcagttt aggctctgaa aaataagaat tcacttttat tagtccaatc aaacacaaat
    10081 accgttttaa gtagcttttc ctctacttaa aacggcaata tgaaagaaac tataactttt
    10141 tattcaatct tttatgttta actggtttat cgccaatttt ttaaaacttt gattattcct
    10201 tatattataa gaatagacat taagtgaact aattcaatca tactgattct ttcgtgtatt
    10261 caaaattaga tgctgtttaa taaaattatt ctttttgaaa aagaattata actatatccc
    10321 aaaaattgta ggttaattta aagcctattt catcatcgtg attgagcttc tttttcatag
    10381 acgaatttct cagatagttt agttttcttt atgagatttt tgtatatttc tgaccttcga
    10441 tacaactcat tatgagtacc ttgatcaact agttttcctt gatccatcac tacaatttta
    10501 tttacctttt caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt
    10561 aaacgattta aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt
    10621 tcatctaaaa ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt
    10681 cgctctcctc cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt
    10741 agtttttcaa taaagtcttc gcagttagct aaacgagctg cttttttcac ttcctcatct
    10801 gacgcttgac tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc
    10861 tcttgaaaaa caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca
    10921 tacccatcaa ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag
    10981 atactagttt tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc
    11041 gtaaaagaga tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc
    11101 aactcaatgt caaaagattt cagaggacta tctgatccct cttggatact ggtttccttc
    11161 atagctctaa ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc
    11221 atagaatcga aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt
    11281 atagtcactt cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta
    11341 acatatgaga aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa
    11401 tgaatccttt cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga
    11461 ttaaagctat taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttctct
    11521 ctcaaagtat cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt
    11581 aagacaaaac taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc
    11641 aaaagcatca ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca
    11701 tgactcatcg catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat
    11761 aaattatgtt ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact
    11821 cttaaatgcg cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac
    11881 aaccaataca tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt
    11941 gtattttcta aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta
    12001 atgcagtata caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca
    12061 cttaaagcaa atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt
    12121 ctcataatta gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt
    12181 tccacgttct actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct
    12241 ggataatcga tgagctatca taataactgt tttttctttc atcagatgtt gaaaagcctt
    12301 ttgtagttca tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat
    12361 ctttgcatct tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag
    12421 ataaactcct ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga
    12481 ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc
    12541 tatagaaacg ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc
    12601 aattgtgtta ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat
    12661 ctgtccctta tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct
    12721 tcctgatttt cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc
    12781 taaaacacac tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt
    12841 aatctgtgtc tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc
    12901 tagtgtttcg actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat
    12961 gaaagaaaca aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat
    13021 cttgggagtg gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat
    13081 gccaaaaaat agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata
    13141 tgcaaactta gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc
    13201 accaaatatc tttataactt gaattccgcg gatatattct accgtttcag agcttaacac
    13261 atctaacgat tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa
    13321 gaatacacca attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa
    13381 gcctaaggca actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg
    13441 agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga
    13501 agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct
    13561 caaattagtc tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa
    13621 taaagctcct aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc
    13681 atctcctaca ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt
    13741 taatacgaca gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg
    13801 gacataacta aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt
    13861 ctcttattcg tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa
    13921 cagataaaag gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca
    13981 atacaccaat catagataaa cttgctacaa ctaacactga aatgattccc catataccta
    14041 ccgcatagtg gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt
    14101 gacgaaaacg atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt
    14161 gcattagagc ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa
    14221 taatatcagc taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc
    14281 ccattaacgt aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag
    14341 ggtagttata gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg
    14401 atatgctgtg accaaacgcc ccaccaatac tactaaccat tattgaaaaa aagtaaatta
    14461 ctagggaaat aactgtagct aaaccaataa aaatataatc ttttgttttc atatatattc
    14521 tcctcaattc tatttcgtta tgtcattaaa aatctgttct atcaaagttg aatcttctac
    14581 tctaagtttt atatcttttt ttattccacc attttttaaa taaataattc tattgcatgt
    14641 tttataaaga aattccggat catgagtagc tataactact gcaccctttt cttttaagtc
    14701 tttaataagg tcagcaatcc tttccatatt aatataatcc aacccacttg taggttcatc
    14761 aaatagaaat aaattagttt ttgacaatga agctatagaa accaataatc tttgtttttc
    14821 tccaccggat aaatcaaacg gtaattgatc tttcatttta taaagttcca ttttttttaa
    14881 aattttttct tgctgatctt tactaactat ttcattcccc agggctaact cgctcctcac
    14941 tgattctgtg aaaaattggt aatccatttc ttgcattact aaaaacggcg atgacaaatt
    15001 ttttttattt tcgattatct ttcctttggt agggcgaata atcttcatga gtattcttag
    15061 taaggttgtt ttccccacac cgtttttccc taccaaacca ataacatctc caactttgac
    15121 atcaaaattt atgtctttta gaatattttc ataagtaatt tcttcaactt ttaatacatc
    15181 tgcacttacc ttttcgatat agggaacttc aatcgaacta ttaaaaatgt caaatttcct
    15241 taatgcgtta ttgttttcag tttttaacaa ctgaatagtt gtagttttta cagttctatt
    15301 tttaacaaat agaacttgat caattaattc tttcaaatag taaaaacgat gttcagctat
    15361 aacaatagta aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc
    15421 tttgaagtcc aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa
    15481 actacatcca atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc
    15541 taatatattt gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc
    15601 ttttgtaaat ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa
    15661 gaattgtcct ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt
    15721 taaggattgt agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag
    15781 tcttggaata agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc
    15841 agtaatcaat gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc
    15901 cttaacatcc ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa
    15961 tcaaaatact taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca
    16021 tgtttgtttt atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat
    16081 tagcggtcat ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt
    16141 ccaaatattg cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt
    16201 taatcattct tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta
    16261 acattatttt tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat
    16321 agacactaac caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata
    16381 aggatgtcgc aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta
    16441 ctaaaaacag aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca
    16501 tcaatactaa aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta
    16561 aaaaaataat gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg
    16621 acactctttc acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac
    16681 cgttattcag ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc
    16741 aagctttttt taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc
    16801 ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg
    16861 tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat
    16921 tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttc gtcacaaatg
    16981 atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat aaaatcaaat
    17041 cgaatatacg tttattctca aaaatataat taataaatgg atctaattca ttgaagttct
    17101 ctctccaact tttctctata tctatatttt ctaagtttaa gaggtatttg gtaaactctt
    17161 cattatacat attaataagc gcattaatat gcggctgaat aatttcttca aataacgctt
    17221 ccttagaagt aaaatgttta taaaaagcac ccgtagttac gcctgcggtc aggcacagtt
    17281 gccttatctt agtttgctga aatccttctt tttcaaaatg gcttagtcca ctttctatta
    17341 acttttggtg agttataata taatctctgg tttctatggg tacttcctcc taataagatg
    17401 aattaacttt attatcttac acaggacttg aagtttcaat cgttaacgtt tattactgaa
    17461 ctcattcctt agagctaaac taatgagaaa gagcagaata actgatacaa aaagcaatcc
    17521 tacaagaatt aatagactca tctaaatcat tcaccttttc taaaataatc cgagaaagat
    17581 ttaaaaaaat tatatccagc tattaggata agtcctatca taatcactcc aatagccatt
    17641 ttcttatcct ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa tcgtcaataa
    17701 attttttccc tttatcagtt aattgaaatc ctatatctaa atagtcctca tatctaagtt
    17761 tagccagcat ctcacttaca gatgctggac taacacttag aacttttgct aaatcattat
    17821 tttttaattt cttttttctt tttgataatt cctgaattgc tagtaaataa attagtatag
    17881 tagtcgtttt aatttgtacc atacctaccc tccaaattat ttcgaaaggc cttcagcaat
    17941 tttatccagg ttccatttca tcatcgcata gtaactatca ccatcttgtc ctttttctgc
    18001 aattgaatca gtaaaaatcg ttgaatagat aggaatattg gtatcttttg atactgtttt
    18061 cattggtcta tcatccacac tgctttctac gaataaggag ggaacttttg ttgtgcgtaa
    18121 tttttcaact aagtgtttta tttgatctgg tgttccttct tcttcagtgt tgatttccca
    18181 aatgtaagca gaaggcacat tatacgcttt cgagaaatat ttaaagcatc cttcacttgt
    18241 aacaatcatt tttttatcat tcggaattga agcaaattta gatttagctt ctttgtctag
    18301 agaatccaat ttttcaatat acttatctag attttcttta tagaattttt tattatcagg
    18361 atctttttcc gctaactgtt tttcaatatt tttagcgtaa ataataccgt tttctaaatt
    18421 taaccaagca tgaggatctt ccttcccttt ctcactctga ccctctaagt aaataacatc
    18481 tatgccatca cttgctgcaa aatagtcttt gttttcctct ttgttcgcat ttttaactaa
    18541 ttttgtaaac caagcatttc ctccagtttc caagttaaca ccgttataaa aaatcaaatc
    18601 tgcttttgaa gttttttgaa catcttcagg caaaggttca tattcgtggg gatctttccc
    18661 aataggtacg atactgtgta aatcaatttt atcttttgct atattttcgg taatatctgc
    18721 taaaatcgaa ttagtaacta ctacttttaa tttttcgttc gaagccactt tatctttgtc
    18781 tttactattt attgaacatg ccccgaatat aaaaagagca atcaaaatcc ccagacttcc
    18841 cagtactgct ttccattttc tcatcataac gatctctcct tctttcttaa aaatttttgt
    18901 tttggtgaga ttagaaaact aattacaaat aagaatgccg ctgttaaaac aatacttgat
    18961 cctgctgcta tgttaaaact ataaccaata aacaatccaa gcaatgatga acttgctccg
    19021 actgttgcag aaataaatat catagttttc aagctatttg catataaata tgctgtagct
    19081 gctggtgtaa ttaacattgc aacaattaat atagttccca cactttgcat agctgttact
    19141 gctactaaag ttaatagaac catcaataga taatgataaa aactgacatt catccctatt
    19201 gcttttgcca ttaatggatc aaaagaagtg attaataatt ctttaaaaaa tagaactact
    19261 ataaccaaca ctaaaaccga aacgccaatt gtaatccact tatcaatatc ttgtacagct
    19321 aaaatatttc caaataaaat atgaaataaa tccgttgagc tatttgcaac tcctataagg
    19381 ataactccta gtgctaaaaa agaactgaaa gtaataccaa ttgctgtatc tccttttatt
    19441 acactattgc tttttataaa agtaataatg atagaagcta atagaccaaa tacaatcgca
    19501 ccaataaaaa aattaatacc taaaatataa gaaattgcta ctcctggcaa aacagcatga
    19561 gaaatagcat cacccataag agacattcct cttaaaataa taaaacaacc tactgttcca
    19621 gaaacaatcc ctataactac agatgtaatt agagcattct gtaaaaagtg aaagtcatac
    19681 agaccattaa taaaattttg aagcatatta ttccccctct ccaataaata tagtatctcc
    19741 ataggctttt tttaaatttt cttcattaaa tacagattct acacttccgt gagcaattag
    19801 atttcgattc aaaagaataa tattatcaaa atacttcttt accttgctta aatcatgatg
    19861 cacaattagc actgtttttc cctgatgttt caattttttt aacgtatcca taattattcg
    19921 ttcactaact aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg
    19981 agcaagacat cttgctaaaa gtacacgttg aaattgtcct ccagataatt ctcctatttg
    20041 atgattagaa tattttccca tatcgacctt ttccaaagcc tttgaaactc tttgccattc
    20101 tgcattttta attctttgaa aaactttcat tccggcatat gttcccatag atacacattc
    20161 ttttactttt attggaaaag taaaatcaat atcagctttt tgctctacat atgcaatctt
    20221 caccagtttt ttattgatat cttcattatt aatggagacc tctccctgat gagggataat
    20281 atttaacatt gctttaagca atgtggattt tccagcacca tttggaccaa taatacctgt
    20341 taaagcacct tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca
    20401 aacattaagc tttttcactt ctaacataac tttctccttc taaatgataa tgattatcac
    20461 gatcatatgg tatacaaaaa ttatatttta gtcaacccta aattttatag tttagatttt
    20521 tttctgttgc tgaagattag aatgacaaaa ctatttgaaa ctacgcgtat agtaagtagc
    20581 tttcaaaata tataaaaaag gagcgttctc aaatgagaac atgctcctaa aactacttta
    20641 ttggcgtatg gaaaaattat acttgaagtg gatagcgggt tgaataagtt gataagcatc
    20701 aaaacattta taaaaattat tcaagactat gtctgttcca tatacaaata tgaaaatcag
    20761 taaactccat ttaacttttt tcgtagtgtg tctttttata catgtttatc gttattaata
    20821 gtgaatagga aaaaaactat ttttgatcat tgacaattaa atacaatttt tgaataacga
    20881 tccctgctat tcgagctaga aactcaagta cgccatgacc tgattagcga caaatactta
    20941 gagatttctt tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa ataatgatac
    21001 ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt acccaaagca
    21061 attcaaaaat ttcataacta ctattttagg agatgatttt atagaatcca gagtctacct
    21121 gaataaaaaa caaacctgtg catttctagg aatcgataat aatactctag ataagtggat
    21181 tcgtatatat aatttcccag taataagagt tggtggtgta gttcgctttg acgtagaaga
    21241 tattaaagac tttatgaata catttaaaat aacttgaaat tttcttctcg ccgcatatac
    21301 taaaattagt gaaatatact gccttacttt ttttaaggag tgaggatttt gcctacaaag
    21361 ctttcaaatg ggaaatataa aactaatctg agatatccaa aaaggtttaa agaaataaca
    21421 ggaattgcat cagaaaagta tcaaaaaaca tttccaaata ggcaattggc gattaaagcc
    21481 gaaaatgata tgaagaagaa aatagaaaaa gttttgagag aggagaatgc aaactcatta
    21541 gaattaaaag gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct tcctagatat
    21601 gaattaggtc aaacaattag atcaaacagg cctcctagtg atataaccat tagtaataca
    21661 aaagatatat ttcggttgca tattttacct atgtttggtg aatacgcaat gaattatcta
    21721 aatatcaata ctgagataat atcagatgaa ttaaccaaaa aatcgaaaga atatgcaaat
    21781 ataaaaatca taaaaggata tgttcgttcc atgttcgata ttgcagaaat actaaattat
    21841 atcgaattta atcgaactac taaaattatt caaagtatca ctgcacctaa aaaaaatgct
    21901 ttagaagaaa aaagaataca agaaggtaag caagcactat catcaaaaga acttactaat
    21961 tggatagaag cagtaaatga tgattttaat aatcatttac ttacttttca tgattacaca
    22021 ctttttatga ttaccctgta tcttggtgat agaaaaagtg agacttacgc tttgcaatgg
    22081 aaatatattg attttgaaaa acaaacggtc cgtcttaaac atactttaga taaatatcaa
    22141 agaaaaaagt ttacaaaagg tcgaaaagat accgtcattc aagtaccaga agttgtaatg
    22201 acattgctaa gtgaatggaa atcggtacaa gctgatcaat tgttgaaatt aaaaattaag
    22261 caaacgttag atcagtatct ctttacttat actaaaccat ctggagaagt aaattgtcct
    22321 gttcatgccg attacctaaa ctatcgaatc aattcaatta aaagaaggca tcctgactta
    22381 gttcatttat ctccacacaa actccgtcat acttatgcaa ctatagctag acaaggtgga
    22441 gcagatatga atcaaatttc aaatgcttta acacactcag atatttctac aacaaagata
    22501 tatgtgaaca caccagatat agttgataag gctgtttttg aagcttttca gagaggattg
    22561 aataagtgtg attgatatgt gttcttaaat gtgttcttaa aaacaaaaga agcactttcc
    22621 aaataattga aaagtgccag aaacgttgat atataaagga tattaacgtt ttgagaattg
    22681 tgaagcttta cgtgctttct taagacctgg ttttttacgt tcgaccatac gtgcgtcacg
    22741 agtaagtaag ccagcacgtt taagagctaa acggaagtca ggatcaactt ctagcaatgc
    22801 acgagcgata ccatgacgga tagctcctga ttgtccagcg tatcctccgc cgtttacgtt
    22861 aacaaatacg tcatatgcgc cttttgtttc agtaacagcg aaaggttgat tgattacttc
    22921 acgcaagtca gcatgtggaa tgtattcttc aacatctttt ttatttacag taattttgcc
    22981 agttcctggt actagacgta cgcgagcaac tgcgttttta cgacggcctg tgccgatata
    23041 ttgaacttgt gccaatgaaa ttccctccta ttagattaag tttgtgatgt ctaaaacttc
    23101 tggttgttgt gctgcatgtg gatgttctgc tccaccgtat acgttcaatt ttgtaaattg
    23161 tttgcggcct aaagtgtttt taggaagcat accttttaca gaagtttcga tcaaacgacg
    23221 agaattttta gcacgcaatt cgccagcagt gattgatttc aatcctcctg ggtagttgct
    23281 gtgacggtaa taaattttgt cagtcgcttt tttacctgtt aatttcactt tgtctgcatt
    23341 gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt tatttttacc
    23401 acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag ttgcgtctac
    23461 tacataccat ttacgttcta cttcgcctgg tttggccata tatgttgtac gcacgttatt
    23521 ttcctccaat ttaagttctg atgtttgaca tacacttcga gtttccgggg ctctttgtgg
    23581 ggcaaacaat accatttaac atattacccg ctaatctagt caatgtcaac gtttttctgc
    23641 ttgattgaaa aatttatatg aaactttact tcttttcttt cttttatata aaagggtcgt
    23701 ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac aaccccttat
    23761 tttgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccataaaac
    23821 ataagaagct gttttttaaa attgtttctt gtgactcgga gctgaacggc aggcttacaa
    23881 cctcttggta aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    23941 tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatttcaca
    24001 acctcttttt cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc
    24061 aaatgagctt ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata
    24121 tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct
    24181 gctagataac gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag
    24241 cggccaattt catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt
    24301 gaaccgtcta atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc
    24361 atgaactcat ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt
    24421 gtttggtgaa caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct
    24481 ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata
    24541 gtagactttc aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct
    24601 tttttatccg taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa
    24661 attgctgata agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct
    24721 gatatttttt atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct
    24781 gtgcttcact gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta
    24841 atgcggatgc ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca
    24901 tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat
    24961 tttcctggca aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg
    25021 ccttcatcat ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat
    25081 ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg
    25141 cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt
    25201 ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag
    25261 caccattgct agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg
    25321 tctccatttc tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc
    25381 ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc
    25441 cgtctgcccc tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt
    25501 ttgatggtag acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc
    25561 gaatgttcaa gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc
    25621 cagttcctga agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat
    25681 agtctttgat accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca
    25741 gccacttgat tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa
    25801 attctttgcc aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg
    25861 cccaagtgat cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca
    25921 tcgcagagga gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt
    25981 cttttataac ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt
    26041 gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg
    26101 tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt
    26161 tttcacctca aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt
    26221 gaaaaggggg agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt
    26281 tatgtttaac cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt
    26341 gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg
    26401 tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag
    26461 aagccttatg tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt
    26521 cgacgttcat atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca
    26581 tggtccattc tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg
    26641 ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc
    26701 gctatggtca atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg
    26761 gcttgtctct agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg
    26821 ttttttctgt ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt
    26881 ttttgagcag tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa
    26941 acgacttgtc acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc
    27001 taggcgacag gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt
    27061 agctcaaaat gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg
    27121 cttcttattt cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc
    27181 gataaagatt gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag
    27241 gataaaattc gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt
    27301 cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga
    27361 tcactggacg ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg
    27421 tttgctgcca atagatttcc accaactgat cataagaaat aacttctgga tcatattcga
    27481 tctctactgc ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg
    27541 gtacatgccc gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg
    27601 gtttgaccat acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc
    27661 cccctttttt ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag
    27721 aaaaaacgga aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac
    27781 ttgatcaaaa acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc
    27841 acctattccc aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc
    27901 ttcaatgacc aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac
    27961 ttctggatca ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact
    28021 tgtcaaattc gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat
    28081 cttgatgttt gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt
    28141 ttcatccatt ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga
    28201 tttctctttt tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga
    28261 ctcggttctg gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag
    28321 ctggttagct aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc
    28381 atctaaatcg aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact
    28441 tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa
    28501 gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct
    28561 gctgcttttt cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct
    28621 ggtactcttc tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt
    28681 ccttcgccta cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc
    28741 aatacgtagt cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc
    28801 attccaccac cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa
    28861 aaatcatgca tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt
    28921 ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca
    28981 gtgattttgg ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta
    29041 cctagatttt ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct
    29101 agaccttctg ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg
    29161 atgtgtcgaa caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat
    29221 aacctaagtg gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt
    29281 tcaaaaatcg cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca
    29341 ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt
    29401 ccttctcctc catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga
    29461 aaacctgatt gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct
    29521 ggcgcaatca gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct
    29581 ggtattggtc tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta
    29641 tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa
    29701 ttttttccca tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa
    29761 tacctgaaaa accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc
    29821 cgtcatctga tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat
    29881 gatacgccca ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca
    29941 acgatgagtc gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt
    30001 gagaaaaata ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt
    30061 ggagttcatt tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt
    30121 ctttgtaagt ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca
    30181 gctgttcttt gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca
    30241 aaaaaagcgg atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat
    30301 agttcgtata tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt
    30361 gtatctctaa atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat
    30421 gaatgagcgt ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact
    30481 cttgaacggc ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt
    30541 cttgtcttgt tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag
    30601 tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc
    30661 gtgcgccttc taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact
    30721 gatataaaaa gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac
    30781 cttcgccagt tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa
    30841 tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga
    30901 tcgttatccg atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt
    30961 ctgtataact aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct
    31021 gaagagaggt agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct
    31081 tttctgaaaa ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga
    31141 atttagctgc aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca
    31201 aatcgtcacc aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt
    31261 gctgtccatc gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga
    31321 ccactttcag ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt
    31381 ccactttttt tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta
    31441 aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt
    31501 cgatttttat ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc
    31561 ctaaaggtcg gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt
    31621 cctcctggct tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact
    31681 tattgatata ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc
    31741 tgtttctgcc gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc
    31801 atctgcaatc atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag
    31861 ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc
    31921 ttcgaatagt tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt
    31981 cagtttgtct tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt
    32041 ttctaattcc agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt
    32101 cccgaatttc cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct
    32161 ttttacttta ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc
    32221 gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc
    32281 agaccgacga agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt
    32341 cttgatcatt gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc
    32401 gtttttatcg gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt
    32461 ctcgcacatc actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg
    32521 attgggtgta cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga
    32581 tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg
    32641 atcaaccaca gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac
    32701 tttttctgct acttgtccag cgcctatgat tgctactttc ttcatttatt tctccctcct
    32761 ttgcttattt acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc
    32821 gctgtttcca gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt
    32881 tctcctcgca aacgacattc atataccata taaccttcat actcattttg aagtagcgtt
    32941 tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct
    33001 aaatggatat gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct
    33061 tcaatattca tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca
    33121 atgtaatgac gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc
    33181 tccaaataaa taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg
    33241 ctgactgctt tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa
    33301 gtgaacattc cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca
    33361 cgcaaaatct cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg
    33421 atccacccat cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc
    33481 tgtttgactg tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat
    33541 ccacagtctt tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct
    33601 tgcgtaccga atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt
    33661 ctgggtgctg gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg
    33721 gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct
    33781 cgattcgttt acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa
    33841 agacgatttt tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag
    33901 gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc gatccctctt aaagaagctt
    33961 gtaatgcggc agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca
    34021 attcctttag acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt
    34081 atttttctgc taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac
    34141 ctgctttttt agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta
    34201 cagcatcacc tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat
    34261 cgtaacatac tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat
    34321 caacaccgtt tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt
    34381 tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta
    34441 attgaaattc gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc
    34501 gccattcttc tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt
    34561 ctgttcctgg ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct
    34621 cacggtcttc gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca
    34681 tcttttcttt cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct
    34741 ttaataaatc gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa
    34801 gcggccatca ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt
    34861 ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac
    34921 gaaaccataa agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg
    34981 atccgaataa tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca
    35041 gtcggaatcg tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg
    35101 accatcgtga taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta
    35161 aataatggaa caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg
    35221 attttcgagc gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc
    35281 actactgcag ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg
    35341 aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga
    35401 aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg
    35461 atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt
    35521 ttcctgctct ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg
    35581 ctgcaataat gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg
    35641 caaaggctgt ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga
    35701 catctgtaaa catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg
    35761 gtttgattgc cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt
    35821 cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg
    35881 catacggata gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct
    35941 ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga
    36001 cgattccata actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg
    36061 taacataact taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa
    36121 gcaaaatagc tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag
    36181 caactgttaa acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc
    36241 ttaatccgat aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct
    36301 gaatattgga aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta
    36361 aaagtcccat ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc
    36421 cctgataatc tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt
    36481 atttcgtatt tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta
    36541 cttggttcac tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat
    36601 tcttttcccc gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg
    36661 gcagctcgcc aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt
    36721 gtttattcac tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg
    36781 gttgagataa gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag
    36841 aagaaatcgt caatccagat actgtgccat agacagcttg atctttttgt gttgggatag
    36901 caaaaccgat attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat
    36961 agattgccat tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag
    37021 agcctggcat cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt
    37081 ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa
    37141 attgagagaa cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt
    37201 gtttattctc tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg
    37261 gttctgaaaa accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc
    37321 cgctaattgg tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc
    37381 cgtcataata attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa
    37441 tcagttcatc tttatccatc actttagcaa aatcctgact gacttccatt acttctggta
    37501 atttaccgga acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca
    37561 cttgcttgat tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca
    37621 cacttaatcg ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt
    37681 cgttagagct ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg
    37741 tagtaacaat aatttttttc atttttttcc acctcttatt ttattatttt cagccacata
    37801 tattcatacg gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa
    37861 aacaggtcta ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta
    37921 tggataacta agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct
    37981 gagtccagta agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat
    38041 agcatctggg tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc
    38101 tcaatatctg ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct
    38161 acgcctttgt aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct
    38221 ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta
    38281 tctgttggag aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt
    38341 ccatctgggt ttttcttgta gtttactcgg gtaccctctt tcttcagctg ttccactaat
    38401 tcaaggatcg ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg
    38461 gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat
    38521 tctttcaact tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa
    38581 aactgataga ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg
    38641 tgtggcacat ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca
    38701 cggaataatt ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct
    38761 ttccataaaa atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac
    38821 aaaagaatat cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga
    38881 tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta
    38941 agtaacgggc tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca
    39001 ataaaaaaat ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg
    39061 gcagacatat gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct
    39121 atatcttccc atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca
    39181 tcatcagatg tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa
    39241 tacttttgat aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga
    39301 tccccataag taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct
    39361 ttcttctgct gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa
    39421 tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt
    39481 tgggagcgtt cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt
    39541 tttaaataat tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa
    39601 tagcttctta aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa
    39661 tcatagacaa aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag
    39721 gaggtgcata taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca
    39781 aaatcaactg tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca
    39841 agtgtacttc aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg
    39901 tcaggaaatg gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat
    39961 ggcaatcctt ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac
    40021 ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa
    40081 aagatccaag aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca
    40141 cttctttttg aattggatca atacaatatt cctattgtgg taatcggaaa agtcggaaac
    40201 gactacacga acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg
    40261 gaaacactga tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt
    40321 tatgtatcca atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat
    40381 atccgtaatg aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg
    40441 aatgatttaa tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa
    40501 tcattgagta tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg
    40561 atcgcaggat tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata
    40621 gacattccga caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac
    40681 caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg
    40741 atccaacaac aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc
    40801 agttactctg ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg
    40861 aatttttctt attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa
    40921 actatttttt ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat
    40981 agttcgtttt tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat
    41041 ctctaaaaca taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta
    41101 ttcaataata gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt
    41161 gcattcaaaa aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt
    41221 tccgatacct taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta
    41281 gttagacaaa ttattttaga caaatcgagt agaggggaaa gccaactgac cttccgcccc
    41341 tctcacacca ccgtacgtac ggacccgtat acggcggttc aataagttga gtgtacgaac
    41401 tggtacttca aggagatgtc ctcatatcct tggcgggcca gttctttgtc tgttaatgat
    41461 cgatgaagaa tatgactttt ggagactgac caaatacctt ttcttgtatt tgaccattca
    41521 tacgctttcg ctttatcgat ccctaaacga atcagatttt ttcttttggt tcttggattc
    41581 ttccattgtt tccaaatata ttgtctgatt cttcgcttca accaaccgtt taactccctc
    41641 atgaattctt tcatctcacc aattccatag tagttgatcc aaccagtcat tagttgcttg
    41701 atttctttta aaataagttg gatacttctt ccacggtttc ttttagtgag ttgtttgagc
    41761 ttgcttttca cacgctgttt tgcttttgcg tgagggcgaa ttttaatacc tttctttgtt
    41821 ggcagaatgc agaatccaag aaactttcgt ttcaatggac ttcctaccgc actcttttct
    41881 cgatttaccg tcaactttag ttgttcttcg agaaattgaa tggatttctt catcacacgc
    41941 tgtcctgctc gtttgcttct aacatagata ttgcagtcat cggcgtatcg aacaaattta
    42001 tgtcctagat tttctagcac ttgatcaaat tcgttcaggt agatgttact taacagggga
    42061 gagagattgc ctccttgggg cgttccttct tcagttgttg tgatgagtcc attttccatt
    42121 acaccactca ttaagtattg acggatcagt tttaggactt gtttgtcctt tactttccgc
    42181 tcaatgaaat acatcagctt atcgtgattt acggtgtcaa agtaggcttt catatcaatg
    42241 tccaccacat atcggtaacc ttgttcataa taagattttg ctttcttcac tgcgtcatgt
    42301 gcgctgcgat ttgggcgaaa cccataacta ttatctgaga attctggttc aaatagcggt
    42361 tgtaacactt gattgattgc ctgttgaagc atacgatcaa taaccgtggg aattcctagt
    42421 tttcttacac cgccgtttgg tttcggtatt tctactcgca aaacaggctg aggtttatag
    42481 ttcccttggc gtaaattgga tagtaattcc tcgcggtgct ctttcaagta aggaagcagt
    42541 tgatcgacgg tcatcccatc tgcaccagcc gcccctttat ttctgactac ttgaagatac
    42601 gcttgattga gattattccg atctagaatt ctcccaaaca gattttctac accatcttgt
    42661 ttcctctgtt caccgcaaga agtgctgcgc acttcatcat acccttcgtg ttccacacta
    42721 gctctttgat gatagccaag ttttcttgtt ttctgcggtc gtcgcatctt ctcacacctc
    42781 ctaaattatt caagattctt attgttcagt ccttcgtaac ggtcagtgtc gttactacta
    42841 tgacctcggc tgacttctca tgattcacca tcatgtcgcc atgatcgttg tgctggtttg
    42901 ttttcattca acgcccacaa caccctcatg agatctcccc cggtaagagt gaaaactttc
    42961 ctcccatgta actgctagat ttactgtata ggtttcgggc agtattggac tttgtcttgt
    43021 gatgaagact tatccgacct aattcagcct tgtatctagt ttctgttcgt cagttcagga
    43081 gtttgcgtcc gacttccttc agccacaacc tcacgattgc cgccttgtct ttcgctaaca
    43141 gttcctactg ccaagcctgt agtggacttt caccaccaag ttttcaccca tgcagggcgc
    43201 acaccaaaaa agaggttgtg acaataattt gtcacaacct cttttttaaa atatctattt
    43261 tgtttgattg ttcattgcgt cttctgcagc catttgcgct tcaatatctg aaatactgta
    43321 gacattttcg cttgctacac cgacttcttt tggttccatg tcacgataac gttgcatacc
    43381 agtaccagct gggatgatct tacctataat aacattttct ttcaaaccaa gaagaggatc
    43441 tttcttaccg cggatcgcag catccgtcaa gacacgagtt gtttcttgga atgatgcagc
    43501 agataagaaa ctatttgttt ctaatgatgc ttttgtgata cctagtaaga caggtcgaga
    43561 agttgcagga acgcctcctg agactagcgt attgtagttt tgatctttaa agtctgcgat
    43621 gtctaataat gtacctggta agatatctgt atcacctgga tccattacac gaactttacg
    43681 cagcatttga cgaaccatta cttcgatatg cttgtcgccg atttctaccc cttgcatacg
    43741 gtaaacacgt tgtacttcac gaagcaagta gttttcaaca gataatacat cgcgaacttg
    43801 caacaattgt tttggatcga ttgatccttc tgtcaatggt gcaccacggt ggatcatatc
    43861 gccttctgct actttcatac gtgctgtata aggaacagta tacgtacgtg tatctgtttt
    43921 tcctttgatt gttacttctt tggtacgtgt tgcaggatct tctgagatat cgatgacatc
    43981 ccctgttact tctgtgatca ctgcttgccc tttcggatta cgtgcttcaa agatttcttg
    44041 gacacgagga agaccttgag tgatatcgtc tccggcaacc ccacctgtat ggaatgtacg
    44101 catcgtcaac tgagtaccag gttcaccgat tgattgagcg gcgatcgtac caactgcttc
    44161 tccgacttca acgtcagagc cagttgcaag gttgcgtccg taacaatgtt tgcagacacc
    44221 atgttttgta ttacatgtga agacagaacg gattgtcact gtttcgacac ctgcatcaac
    44281 gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct gcaatgatca attcatttgt
    44341 ttcaggatga cgtacttctt tacgggtgta acgtcccact aaacgatctt ctaatgattc
    44401 gatgatttca tttccctcgc ggatggcttg gatttcaaga cctcggtcag taccgcaatc
    44461 ttcttcacgg atgattacat cttgggccac gtcaaccaga cgacgagtca agtaacctga
    44521 atcggcagtc ttcaaggctg tatcggtcat ccctttacga gccccgtgag ttgagataaa
    44581 catttccaag acggatagtc cttcacggaa gttcgaaatg attggcaatt ccatgattcg
    44641 accattagga gcggccatca aaccacgcat tccggcaagc tgtgtaaagt tggaaatgtt
    44701 accacgagct ccagaatcag acatcatgaa gattggattt ttcgcttcca aaccttccat
    44761 cagtttttgt tggatgctgt cttttgcagc attccaaaca gcaatgacac gttcataacg
    44821 ttcgtcatct gtgatcagac cacgacggaa ttgttttgta atagtttcaa cttgtttatg
    44881 ggcattttca atgatttctt gtttttcatt cagtacactg atgtcagcga tccctactgt
    44941 gataccggca tgtgtcgaat gtttgtaccc taagtctttc atacggtcaa gcatcttaga
    45001 agtttctgtt actttgaatc gtttgaagac ttctgcgatg atgtttccta agtttttctt
    45061 cttgaacggt aatactaatt cttgctcttt gatatgtgca ggaatatcag taccagcttc
    45121 aacaaagtat ttgtccggtg tttggacggt caagttgaag tctgttggtt cattcagata
    45181 agggaattca ggaggcatga tctcgttgaa gatgatctta ccaactgttg tgatcatcgt
    45241 acgttctttt tgccattcag taaatggttt ttcacctaat gagttagggt tcacaccgat
    45301 acgtgaatgt aaatgaacat atccgttgcg ccatgcgata acggcttcgt tttgatctct
    45361 gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt gtcaagtagt agttacctag
    45421 aaccatatct tgggaaggag taacaactgg tttaccatct ttagggttca agatgttttg
    45481 agcagctaac atcaacatac gtgcttctgc ttgtgcttct tcattcaatg gaacgtggac
    45541 agccatttgg tctccatcga aatcggcatt ataagcttca catacaagtg gatgcaaacg
    45601 gattgcgcgt ccttgtacta aaactggttc gaatgcttgg ataccaagtc ggtgaagcgt
    45661 aggtgcccgg ttcagcaata ctgggtgttc cttgattacg tcttctaaaa catcccatac
    45721 ttcgtcttct tgacgttcga ttttacgttt cgcatttttg atgtttgaag ctagttcgcg
    45781 ttgaacaagc tcacgcatca cgaatggttt gaataattca atggccattt cttttggaag
    45841 tccacattgg tacatcttca agaaaggtcc aacaacgata acggaacgac ctgagtagtc
    45901 gacacgttta cctagcaagt tttgacggaa acgaccttgt ttccctttca acatatgaga
    45961 aagagatttc aatgggcggt tacctggtcc agtaactgga cgtccgcgac gaccgttatc
    46021 gatcaatgca tctactgctt cttgcagcat acgtttttca ttttgaacga tgattccagg
    46081 tgcattcaag tctaacaaac gtttcaaacg gttattacgg ttgattacac gacggtaaag
    46141 atcattcaag tcacttgttg caaaacgtcc accttctaat tgaaccattg ggcgtaaatc
    46201 tggtggaata acagggataa cgtccataac catccagctt ggttgatttc ctgaagcgcg
    46261 gaatgcttct aaaatatcca aacggcggat tgcacgtgta cgtttttgtc cttgtgcaga
    46321 tttcaattct tctttcaatt cggctgcttc tgcatctaga tcaacattat caagtaattg
    46381 tttaatcgct tctgcaccca tagcagcttg gaattcttgt ccatattgtt cacgtttttc
    46441 gcgatattcg cgttctgtca acaattgttt tttctctaaa ttcgtatttc ctggttcgat
    46501 gactacatat gaagcaaaat aaatgatttc ttccaaagcg cgtgggctca tgtctaatac
    46561 aagacccata cgtgaaggaa tccctttaaa gtaccagata tgtgaaacag gagctgctaa
    46621 ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga gtaacttcca ctccgcatcg
    46681 atcacaaacg attcctttat aacggatacg tttgtatttt ccgcaggcac attcccagtc
    46741 ttttgtagga ccaaaaatcc gctcacagaa caacccttcg cgttcaggtt ttaaggtacg
    46801 atagttaatc gtctcaggtt tttttacttc accgtaagac cagcttctga ttttttcggg
    46861 agaagccaaa cctatttgca tactttcgaa tttatttaca tcgatcaaaa ggggttccct
    46921 ccatttcatt taatggcaaa ccgcaagacg gctgatcttc gaccgccttg cagctcatag
    46981 accggattag tctaaattat cttctgcggt ttcaaagttt tggatgacat cgtctttttc
    47041 atcttccact tgttcagcag ctttcttttc taattctttg gcagtttgct gttcggcaaa
    47101 ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca tcgtccatgt cgcgaagttc
    47161 gatctctgtt tcttcgatat ccaagacacg catgtccaga cctagtgatt gcaattcttt
    47221 caccaatacg cggaatgatt ccggtacacc tggttttgga attggttcac ccttgacgat
    47281 tgcttcgtat gttttcacac gtccgactac gtcatctgat ttgtatgtca agatttcttg
    47341 taaagtataa gccgcaccgt aagcttccag tgcccatact tccatttcac caaaacgttg
    47401 tccaccaaat tgagctttac ctcccaatgg ttgttgtgta accaatgagt aaggtccgat
    47461 tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt ttgatcatgt acatcacacc
    47521 gacagacaca cgtccatcaa atggttcacc tgtacgtccg tcgtacagaa cagttttcgc
    47581 atcgctggcc ataccggctt cacgaactgt ttcccatacg tcttcatcac ttgcaccatc
    47641 aaatactggc gttgctacgt ggatacctaa ctgacgtgca gccataccca agtgtaattc
    47701 caatacttgc ccgatgttca tacgtgaagg cacccctaaa ggattcagca tgatatcgat
    47761 tggtgttcca tcaggtagga atggcatatc ttcttccggc ataatacggg atacaacccc
    47821 tttatttccg tgacgaccgg ccattttatc cccttcatgg attttacgtt tttgaacgat
    47881 ataaacacga actaacatgt ttacgcctgg tgacaattca tctccagctt cacgagtaaa
    47941 gattttcaca tcgtgaacga taccgccgcc gccatgtggt acacgtaatg atgtatcgcg
    48001 gacttcacga gctttttcgc caaagatcgc atgcaataga cgttcttctg cagataattc
    48061 tgttacccct tttggcgtga ctttccctac aagcaaatcg ccatcttgga cttctgcacc
    48121 aatacggatg atacccattt cgtctaaatc ttttaatgcg tcttccccga cattagggat
    48181 ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg gcttctgatt catattcttc
    48241 aatatggata gaagtgtata cgtcatcttt taccaaacga cggctcatga tgatcgcatc
    48301 ttcatagttg tacccttccc aagtcatgaa accaactaaa acgttttggc ctagagccat
    48361 ttcgccttgt tccatagatg gtccgtctgc cagagtatcc cctttttcga ctttctcacc
    48421 caaatgaacg attggacgtt ggttgtagct tgttccggag tttgaacgac ggaattttgt
    48481 tacatcgtat ttatctaatg caccgttgtc gcggcgtacg cggatttcag aagcatcaac
    48541 gtattcaacg acaccgtcat gtttacataa caaagcagct cctgagtcat gcgctgattt
    48601 gtattccata ccagtaccaa cccatggtgc ttgaggattg atcaacggca ctgcttgacg
    48661 ctgcatgttg gcacccatca aggcacggtt ggagtcatcg ttttccaaga aaggaataca
    48721 tgctgtcgca acggcaacta cttgtttagg tgatacgtcc atgtagtcta ctttatcaat
    48781 agatacttct aagttttcac tttgagcacg agccataaca acgtcttgag caaatgttcc
    48841 atcttcattc aatgggctgt ttgcttgggc tacgatgtaa tggtcttcga tatcagctgt
    48901 caagtagtcg atttggtcag taacacgacc tgtttcacgg tctacacgac gataaggtgt
    48961 ttcgataaat ccgaatttat ttactttcgc ataactggac aaactattga tcaacccgat
    49021 gtttgggcct tcaggagttt cgatcggaca catacggcca tagtgggagt agtgaacgtc
    49081 acgaacttca taaccggcgc ggtcacgagt caatccgcca ggtcctaagg ctgaaagacg
    49141 acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc atgaactgtg acaactgaga
    49201 agaaccaaag aattctttga tacttgccac aactggacgg atattgatca attgttgagg
    49261 tgtcaatgtt tcagtatctt ggatcgacat tctttcacga accacacgtt ccatacgtgc
    49321 tagcccgata cggaattggt tttgtaaaag ttcaccgact gaacggatac gacgatttcc
    49381 taagtggtcg atatcatcga cattgccgat accttccatc aagttcaaga aatagctcat
    49441 tgaagcaatg atgtcggctg gacgaaccgt ttttacagct gcttctggat agccgtttcc
    49501 gatcacgttt acttcacgtt cagggtctct tggtgaaaat actttgatga cttggacagt
    49561 cattggatct gtgaccacac cgtcttctga tggatagtaa gtcactgaat ttaggccatt
    49621 atcaatgaaa ggagctagtg tttccatgac ttggtgagtc agaactgttc ctttttcaac
    49681 gatgatctcg cctgtttctg gatcaactaa tgtttcagct aatgtcagat tcaatagacg
    49741 tgttttcaaa tctaattttt tgtttacttt gtaacgacca acgtttgcta agtcgtaacg
    49801 ttttggatcg aagaaacgag cattcagcaa gttacgtgag ctgtctgctg tttttggttc
    49861 gcctggacgc aaacgttcgt aaacgtcttt caaaccttct tctgttcttg agtcgcttgc
    49921 gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt tcgctgtcgc cgaagatttc
    49981 aaagatcgta tcatcggaac caaatcctaa cgcacgaact aatacggtta aaggaatctt
    50041 acgtgtacgg tcgatacgta cataagagat gtcttttgca tcagtttcca tttctagcca
    50101 agctccacgg ttagggataa cagtagaacc gaatccttct tttccgtttt tgtcgacttt
    50161 tccatggaaa tagacacctg gtgaacggac taactgagaa acgattactc gctctgcccc
    50221 gttgatgatg aatgttcctt gttctgtcat taatgggaaa tcaccgaaga atacttcttg
    50281 tgctttgatt tcgccagtct cgcggtttgt caaacgcaat gttacatgta atggtgcaga
    50341 gtagtttgca tcatgcgcac gagcttctgc tactgtatac tttggttctt tcaattcata
    50401 atctacaaat tccaaagata gattgccatt gaagtcatcg attggcaaaa tgtcttcaaa
    50461 catttctcta agtccctcat ctaagaacca ttggtaagag tctgtttgaa tttcgatcaa
    50521 atttggtaat tccaacactt cactgattct tgcgaaactt ctacgttcgc gatgttttcc
    50581 gtattttact acgtgtccag ccaagctctt cacccctcta aaatgtcatt caaaaagact
    50641 acccgatcaa ttgttacaat aatatttcaa atattatttt taggtaacaa cagacatttt
    50701 ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt ctcttgaaac cttacttcgt
    50761 gagcttctac ttttgtttcc ttatattaaa gccgaaacgg acaatatttc gtttctgcct
    50821 tttcttcatc agatagtttt tgcaacttca tatactacac ttattttcta taaaagtcaa
    50881 cacttcctgg cgtttcttct gctttttcgc aacattttct taatgttttc aattttatgt
    50941 tttcaattat ctgacaattc ttaattgaca gaccttttta tctcggttat actttagaaa
    51001 attcattttg tggaggaact tctatgcgaa ccaacatcaa ccaaatcgca ccaacacaac
    51061 tgaataactt ttactttagc tactttagat aggtttgtat tcatcatgat ggatacaaac
    51121 gttaaacgac agtttgtatc tatgatggct aaagaacttt gtattgcagc aaaaagccac
    51181 gtagacaaaa tgctaagtgg ctgagcagtg aaaatacaaa gtaaaagtat caagcattct
    51241 actttttcaa tttattttcc gacttttcaa tccatttgga cattttgtct gaatggattt
    51301 tttgtttccc aaaattaagg aggaaaaaga aatgaaaaaa aatataatca tcggattagg
    51361 cgctgccctg ctactagctt taggaggttg cgcacaaaaa gacaattccg cagaggcatc
    51421 agatggacaa aaaacaattg gcgtattgca acttgtacaa cataactcgt tagatgctgc
    51481 ctataaagga ttcaaagaag gattagcaga agaagggtat aaagaaggcg ataatctaac
    51541 aatcgattat caaaatgccc aaaacaatca agacaacctg aaaagtatga gtgagaaact
    51601 cgtcaaagaa gatcctgatt tgctacttgg aatcgccact ccagcagcac aatctttatt
    51661 aaatgaaaca accactatcc cgatgactgt cacagcggtc acagacttgg aacaagccca
    51721 tttagtcaca tccaatgaaa aacctggcgg caatgtgact ggcacgacag atatggtgcc
    51781 aatcgaaaaa caaatcggct tactgttaaa tatcgtgcca gacgccaaaa caatcggcat
    51841 catgtataac aacggtgaag caaactctaa gatccaagct gatttggcag aaaaagaatt
    51901 gaaaaaagcg ggcgtcaaag tcaaagtact gacagcaaat tccacaaatg atgtccaaca
    51961 agtgacgaaa agtctagcta aagatgtaga tggaatctat attcctactg ataacacgtt
    52021 cgcaagtgcg gcaagtgttg tcggtgaagt cgcaaaagaa acaaaaacac ctgtcgttgc
    52081 tggcgcagtt gatcaagtca aagacggcgg tctagcaaca gtaggaatcg actacgaaag
    52141 cttgggtaaa caaacaggga tcatggcagc aaaaatctta gacggcaaga atccaagtga
    52201 acttcctgtc gaaacagcga atgatttgga actgtatgtc aataaagaca tggccaaagc
    52261 attaggcatc aatccagaca gcatcaaaga agctgaataa aacacaggag gattctcatg
    52321 acaaatttgc tctctctttt cttatcatct gcatcgcaag gggtcttatg ggccctactt
    52381 gcgattggtg tatacgtcac tttccgtatc ttggacattg ccgacttgac tgctgaagga
    52441 agctttcctt taggtgcagg gatcgcggca gtaagtatta caaatggcta tcatccattg
    52501 acagcttgtc ttctaggctt tttagggggt gcggcagcag ggcttgtttc cggactgctt
    52561 cacacaaaat tgaaaattcc cgcccttctt gcagggatca tcaccatgac tggtttatat
    52621 tctgtcacat cccgtgtgat gggtgcgccg aacatctctt tattgggtca gccaacgatc
    52681 tttcattggg tcgagtcatt cggcataaca aaagacaacg ctgtactgat cgtcggtctg
    52741 ctttttgctt tgatcgttgt atgtttactt gttttgttct tacgtacaga aacaggactt
    52801 gctattcgtg caactggcga taatctggca atgagcgaag caaacgggat caacacagat
    52861 aatatgaaga tcatcggtta tatgatttct aatggatgca ttgctctttc tggctccttg
    52921 cttgcgcaaa acaacggctt tgctgattta aattctggta ttggaacgat cgtaattgga
    52981 cttgcttcta ttatcatcgc agaagttttg ttacgtaacc agccactatt tctccgtctc
    53041 ttgactgtcg ttttaggtgc ggtcatttat cgttttattt tagcgttagt cttcgaactg
    53101 aatgttgaac caagtgattc gaaacttgct tctgctttcg tgctagtgat ctgtctttct
    53161 ttcccgcaga tccagcaaaa attccattta cctcaaaaaa agaaaggcgg tagtgtcaaa
    53221 tgaatgacgt cttaacattg gaaaatctcc atcatacctt tgaaaaagga acgatcaacg
    53281 aaaatcatgt cctgaaaggc attaatcttt ccttagatcc cggcgacttc gtgacgatca
    53341 tcggtggaaa tggggctggt aaatctactt tgctgaacag catcgccggt acattttctg
    53401 tcgatcaggg tcaaatttta ttgaatggaa aaaatatcac gaaaaaatct gttgtcgaac
    53461 gttcgaaatc catcagtcgc gtattccaag atcctaaact agggacagcc gttcgtttaa
    53521 cggtagaaga aaatcttgct cttgctatga aacgaggaaa aaaacgcggc ttcttccgtg
    53581 gtgtcaaacc acaagaccga acatttttca aagaccagct ggttcggcta aatttggggt
    53641 tggaaaatcg tttatcctca gaaatcggtt tgctgtctgg aggacaacgt caagctatta
    53701 cgttattgat ggccacatta cagcgtccag aactgatctt attagatgaa cacactgcag
    53761 cgcttgaccc tgctacttcc aaaacggtga tggaattgac agaaaagctg atcactgagc
    53821 agaatctcac tgcttttatg gtcactcatg acatggaaga cgcgatccgc tatgggagtc
    53881 gactgatcat gctgcatcaa ggaaggatcg tcgtagatat ccagcaagag gaaaaacagc
    53941 agctcacagt gccccaatta ttagcgttgt tcaaacaaaa cagcggcacg gcattaaaag
    54001 acgatcaagt acttttatct tcataatcag aaagaggatg ggacaaaaaa tgaacgccca
    54061 ccctcttctt ttttctcttt ccttctttcg tttttgcttt atgtatcaac cgttgtttct
    54121 tcgaaaacta ttttgttctc tccttcttcc tgatagaatg gatgggaaag gagcttgatg
    54181 ccatgacaac gaaagacaag gtacttcaat tattgaaaaa gacaccagat tttctttccg
    54241 gagaaaagtt ggcacagcaa ctggaagttt cccggacaag tatttggaaa gccatcaaag
    54301 aattggaaaa ggcaggctat cgttttgagc acgggcctac agggtatcgt tacctgccct
    54361 ctgacgtact agatgcaaaa gaaatccagc aagagctacg gtcgtttatt cctgatttga
    54421 aagtacaaac gatgttgact tctgaatcta cgatgaaaga tgtcaaactg gcagcagctc
    54481 aaggagattc tgtttcgaag ttatttgttg ctgatacaca agaacaagct cgtggacgtt
    54541 ttggacggcc ttttttcgct gaacctggac gaggaatcta catgagtctc ttgctccatc
    54601 cagacaaacg gtttgatgaa ttgcctcaat atacgatcat cgcagccagt gcctgtgtga
    54661 aagccattga atctgtaacc ggtcgttctg cttcgatcaa atgggtcaat gatatctaca
    54721 tggatggaaa aaaagtctgt gggattttat ctgaagcagt cagtgatatg gaaagcggca
    54781 ggatcagtca tatcatcatt gggatcgggc ttaatttttc tgttcctcaa aatcattttc
    54841 cagctgatct ccaagaaaaa gccatctctg tctttcccga cggacaagct agtgtcacaa
    54901 ggaatcagct aatcagcaaa atctggcaag aattgttcac ttatctttct cacccagaaa
    54961 aagcgttgga cttctataaa gaaaaatcat tcgtattggg aaaaaccgtg tcgttcaatc
    55021 aaaaaggaac gacttatgtc ggtcttgcaa aagaaatcgg tgatcatggc gaactgatcg
    55081 tccaaacaaa tgaacagaag atgagtttgt cttctggcga aatcagtctc acatctatcc
    55141 gtacgcaata attatcagat ttcacaaaag gacacctgat ctcatacagt gtcctttttt
    55201 ctgaattctc ttcaaaagac tctccttatt ctatcagctg acagtttctc tatcctctag
    55261 aaaaaggtat tctaaaaata tctataaagc atggtacact ttctttaaat gaatcaatgc
    55321 aaaaaacaag agagtcatca gtttacacga tcaaaagaga tagcaggccg cttccttttt
    55381 caatcttata gatagaccat gggtaacgat aaaaacaaaa aagaacttgc gacttatttt
    55441 gccgtaagtt cttttttagc tagtgcctta atctcttaat cccaacaaac gtctcgcata
    55501 gatcacctga ccagagtgca tagcagcatc atcaataata gaaacgagac gcattcctcg
    55561 tgtgactggt ggtgtccagt tctcgtcaat tacttcatct agctcggaag gttccagcct
    55621 tcttaagtaa gaaatagcaa aatcagcagc tcctcgcaag tatcctagca gatcttctat
    55681 ttgattagct tggatctgct ttgcttgttc cagactatgc ttccaatctt gttcgttatc
    55741 tgctatcgtc atcggaaacc ggctttccca gtcttgacta tgccaaattg gagaagttcc
    55801 tgctaaatca gcaatctgga aatctaattc tcgagcggta tgccaagcca gccaagtcat
    55861 tgatttgatc tgcggggctc tatcttccac tggaaaacga ttggcctcct caatagagac
    55921 gccttcaaac aaacgttcaa accgatcgta tgcccgatcc acactctcgc atagcatatc
    55981 taaataatcc atttattttc ctccttttta tttatcctac ttactttttc ctaattctag
    56041 gatacctact tatatgttac tacacaccct tattttttac actttaacag tcaccatcat
    56101 caatagtttg gagattgaaa gaaaaagata gtgtcgaact atcaattgaa caagaagcaa
    56161 caatatgaac gtttcattaa tattctttaa ataaacaatg atttttcaaa cgaacagttt
    56221 aagactagga gaaaaagagt ataataggaa aatgtttgaa aggaaaggaa ggggaaaaat
    56281 gaaaaaaagg gcaagtttac tagtcctagt tgctttgttg ttcaatatct tttcgtatgg
    56341 tgtggccgtt tcagcggaaa catacattcc taaagaccct gggaatattt ataatgatct
    56401 aactggtaac tatggcttgt taggaattgc tagtcaattc cacgtatttg ctaaaaataa
    56461 gacaactgtg aatgcacata cagacggaaa tatagcaaca catgaacttg atgcaaaaaa
    56521 taattttggt acagagcttc atgaaggttt attgaaccaa gaaataaatt atatacaaaa
    56581 aactgattct ttcgctaatt cttcaggcat acctacaggt agcacaagaa tgaataaatt
    56641 tgtcgttggc agtaacgtgg atgtagcact tcaagatggt catcctacag tcaatggatc
    56701 caggcttgat cacttaactc cagatgattt ttataaagat cgtgctgggc atgaatatat
    56761 tgatttcgag caggaatttg ctaaattgaa tgtagcagca aatgatcttg caacgatcac
    56821 tccagctaaa acatatactg ctgcagattt tccagacatg aacaatcgga caatcgattt
    56881 aactgattta tcagataccg ggtttttatt ggtaaatatc gatgcagaag tgttaacgat
    56941 gaatacgcca cttcaaatca ttaatccaaa tgatcaagta gttgtattta atgtcatcaa
    57001 cagttcttct gcattgaacg tacagtcacc aatcaaatac aatgatcgtt caaatcatga
    57061 aacagaagat ttttcggatg cgaatatctc ttggaatttt ggaaatgaga tgactgattt
    57121 gacaatcagt gctccttttc aaggaacaat ccttgcgcct aatgctacta tccgcattac
    57181 tcaaaaccaa gacggaacga ttataggaac aaatgtgatt ttagatgcag ctaccaatcg
    57241 ttgggatcct aacgaaatat ttatcagcga caatggtaca gatacgacag acacaagcga
    57301 cagtacagat acatctacca caagtgatag ctcagattca tcaacttcaa gcgacagtac
    57361 agatactacc tctaccacaa gtgatagcac ggatacatca gcttcaagcg acagtacaga
    57421 tactacctct accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac
    57481 ta
//
LOCUS       DAYYGY010000008        52677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000008 DAYYGY010000000
VERSION     DAYYGY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.008"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..921)
                     /locus_tag="ACHA52_000521"
     CDS             complement(73..921)
                     /locus_tag="ACHA52_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8939274.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="ACHA52_000522"
     CDS             complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="ACHA52_000522"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8939275.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3422..3601)
                     /locus_tag="ACHA52_000523"
     CDS             complement(3422..3601)
                     /locus_tag="ACHA52_000523"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8939276.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3648..5471)
                     /locus_tag="ACHA52_000524"
     CDS             complement(3648..5471)
                     /locus_tag="ACHA52_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8939277.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5749..8028
                     /gene="helD"
                     /locus_tag="ACHA52_000525"
     CDS             5749..8028
                     /gene="helD"
                     /locus_tag="ACHA52_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8939278.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8121..8768)
                     /locus_tag="ACHA52_000526"
     CDS             complement(8121..8768)
                     /locus_tag="ACHA52_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939279.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8947..9666)
                     /locus_tag="ACHA52_000527"
     CDS             complement(8947..9666)
                     /locus_tag="ACHA52_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8939280.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9797..10948)
                     /locus_tag="ACHA52_000528"
     CDS             complement(9797..10948)
                     /locus_tag="ACHA52_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8939281.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11165..11905)
                     /locus_tag="ACHA52_000529"
     CDS             complement(11165..11905)
                     /locus_tag="ACHA52_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8939282.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11909..12232)
                     /gene="rsfS"
                     /locus_tag="ACHA52_000530"
     CDS             complement(11909..12232)
                     /gene="rsfS"
                     /locus_tag="ACHA52_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8939283.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12289..12882)
                     /gene="yqeK"
                     /locus_tag="ACHA52_000531"
     CDS             complement(12289..12882)
                     /gene="yqeK"
                     /locus_tag="ACHA52_000531"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8939284.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12866..13516)
                     /locus_tag="ACHA52_000532"
     CDS             complement(12866..13516)
                     /locus_tag="ACHA52_000532"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8939285.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13526..13837)
                     /gene="yhbY"
                     /locus_tag="ACHA52_000533"
     CDS             complement(13526..13837)
                     /gene="yhbY"
                     /locus_tag="ACHA52_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8939286.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13853..14962)
                     /gene="yqeH"
                     /locus_tag="ACHA52_000534"
     CDS             complement(13853..14962)
                     /gene="yqeH"
                     /locus_tag="ACHA52_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8939287.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14959..15486)
                     /locus_tag="ACHA52_000535"
     CDS             complement(14959..15486)
                     /locus_tag="ACHA52_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8939288.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15666..16451)
                     /locus_tag="ACHA52_000536"
     CDS             complement(15666..16451)
                     /locus_tag="ACHA52_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8939289.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16444..17313)
                     /gene="accD"
                     /locus_tag="ACHA52_000537"
     CDS             complement(16444..17313)
                     /gene="accD"
                     /locus_tag="ACHA52_000537"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8939290.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17314..18693)
                     /locus_tag="ACHA52_000538"
     CDS             complement(17314..18693)
                     /locus_tag="ACHA52_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8939291.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18701..19120)
                     /gene="fabZ"
                     /locus_tag="ACHA52_000539"
     CDS             complement(18701..19120)
                     /gene="fabZ"
                     /locus_tag="ACHA52_000539"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8939292.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19145..19621)
                     /gene="accB"
                     /locus_tag="ACHA52_000540"
     CDS             complement(19145..19621)
                     /gene="accB"
                     /locus_tag="ACHA52_000540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8939293.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19624..20859)
                     /gene="fabF"
                     /locus_tag="ACHA52_000541"
     CDS             complement(19624..20859)
                     /gene="fabF"
                     /locus_tag="ACHA52_000541"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8939294.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20878..21615)
                     /gene="fabG"
                     /locus_tag="ACHA52_000542"
     CDS             complement(20878..21615)
                     /gene="fabG"
                     /locus_tag="ACHA52_000542"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8939295.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21627..22544)
                     /gene="fabD"
                     /locus_tag="ACHA52_000543"
     CDS             complement(21627..22544)
                     /gene="fabD"
                     /locus_tag="ACHA52_000543"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8939296.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22651..22875)
                     /locus_tag="ACHA52_000544"
     CDS             complement(22651..22875)
                     /locus_tag="ACHA52_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8939297.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22928..23890)
                     /locus_tag="ACHA52_000545"
     CDS             complement(22928..23890)
                     /locus_tag="ACHA52_000545"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8939298.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23900..24340)
                     /locus_tag="ACHA52_000546"
     CDS             complement(23900..24340)
                     /locus_tag="ACHA52_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8939299.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24646..25611
                     /locus_tag="ACHA52_000547"
     CDS             24646..25611
                     /locus_tag="ACHA52_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8939300.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25668..25898)
                     /locus_tag="ACHA52_000548"
     CDS             complement(25668..25898)
                     /locus_tag="ACHA52_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8939301.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25984..26871)
                     /locus_tag="ACHA52_000549"
     CDS             complement(25984..26871)
                     /locus_tag="ACHA52_000549"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8939302.1"
                     /translation="MKLGFIGTGVMGSAVARHLLETGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26960..28183)
                     /locus_tag="ACHA52_000550"
     CDS             complement(26960..28183)
                     /locus_tag="ACHA52_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8939303.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28200..29246)
                     /locus_tag="ACHA52_000551"
     CDS             complement(28200..29246)
                     /locus_tag="ACHA52_000551"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8939304.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29410..31137)
                     /gene="ptsP"
                     /locus_tag="ACHA52_000552"
     CDS             complement(29410..31137)
                     /gene="ptsP"
                     /locus_tag="ACHA52_000552"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8939305.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31137..31403)
                     /locus_tag="ACHA52_000553"
     CDS             complement(31137..31403)
                     /locus_tag="ACHA52_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8939306.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31882..34116
                     /locus_tag="ACHA52_000554"
     CDS             31882..34116
                     /locus_tag="ACHA52_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8939307.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34264..34584
                     /locus_tag="ACHA52_000555"
     CDS             34264..34584
                     /locus_tag="ACHA52_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8939308.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34789..35013)
                     /locus_tag="ACHA52_000556"
     CDS             complement(34789..35013)
                     /locus_tag="ACHA52_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8939309.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35211..36791)
                     /locus_tag="ACHA52_000557"
     CDS             complement(35211..36791)
                     /locus_tag="ACHA52_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8939310.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37192..38568)
                     /locus_tag="ACHA52_000558"
     CDS             complement(37192..38568)
                     /locus_tag="ACHA52_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8939311.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38696..39784)
                     /locus_tag="ACHA52_000559"
     CDS             complement(38696..39784)
                     /locus_tag="ACHA52_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8939312.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39956..40390
                     /locus_tag="ACHA52_000560"
     CDS             39956..40390
                     /locus_tag="ACHA52_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8939313.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40468..41001)
                     /locus_tag="ACHA52_000561"
     CDS             complement(40468..41001)
                     /locus_tag="ACHA52_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8939314.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41095..42273)
                     /gene="mutY"
                     /locus_tag="ACHA52_000562"
     CDS             complement(41095..42273)
                     /gene="mutY"
                     /locus_tag="ACHA52_000562"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8939315.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42266..43063)
                     /gene="recX"
                     /locus_tag="ACHA52_000563"
     CDS             complement(42266..43063)
                     /gene="recX"
                     /locus_tag="ACHA52_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8939316.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43170..44552
                     /gene="rlmD"
                     /locus_tag="ACHA52_000564"
     CDS             43170..44552
                     /gene="rlmD"
                     /locus_tag="ACHA52_000564"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8939317.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44726..45304)
                     /locus_tag="ACHA52_000565"
     CDS             complement(44726..45304)
                     /locus_tag="ACHA52_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8939318.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45626..46096
                     /locus_tag="ACHA52_000566"
     CDS             45626..46096
                     /locus_tag="ACHA52_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8939319.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46204..47430)
                     /locus_tag="ACHA52_000567"
     CDS             complement(46204..47430)
                     /locus_tag="ACHA52_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8939320.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48184..48384)
                     /locus_tag="ACHA52_000568"
     CDS             complement(48184..48384)
                     /locus_tag="ACHA52_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8939321.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48518..49126)
                     /locus_tag="ACHA52_000569"
     CDS             complement(48518..49126)
                     /locus_tag="ACHA52_000569"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8939322.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49211..49681)
                     /locus_tag="ACHA52_000570"
     CDS             complement(49211..49681)
                     /locus_tag="ACHA52_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8939323.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49729..50643)
                     /locus_tag="ACHA52_000571"
     CDS             complement(49729..50643)
                     /locus_tag="ACHA52_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8939324.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50789..51592
                     /locus_tag="ACHA52_000572"
     CDS             50789..51592
                     /locus_tag="ACHA52_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8939325.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51770..>52677
                     /locus_tag="ACHA52_000573"
     CDS             51770..>52677
                     /locus_tag="ACHA52_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8939326.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATH"
BASE COUNT        15194 a        11107 c         9000 g        17376 t
ORIGIN      
        1 tgatggatca tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa
       61 atagtaaggt ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt
      121 cactttttcg ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt
      181 agcaaattgg gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct
      241 agatgtttgt acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa
      301 cgcaatgatt tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact
      361 tctacctgag tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg
      421 gagcaagacg tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg
      481 cagaatatct tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc
      541 cgattccaaa cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc
      601 ttttatcgct acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt
      661 tgcttctata tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat
      721 tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc
      781 actcaacccg gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt
      841 accgataatg tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc
      901 aatacgacta attacgctca taagttcctc caccctttta atgatagtct atgtaagtat
      961 accatatcag acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc
     1021 caacagaata tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg
     1081 aatacctgtt atctattgtt cggattgctt tctatctact acttttttac aggaaattta
     1141 cctattagtt ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat
     1201 tcaaatcttc tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt
     1261 ccaattcctt gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta
     1321 tcttatagcc cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca
     1381 aacgttcttt gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg
     1441 ttgcttgtat aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag
     1501 cgacaaaacg ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat
     1561 tttccccgtc ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt
     1621 gtatagttga taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg
     1681 gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag
     1741 cggctttcag cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg
     1801 tattgtttgg atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat
     1861 atactgccac catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa
     1921 caaatgtatg accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac
     1981 ctgatttttc atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat
     2041 aagaatggat cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa
     2101 caagcatttc gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc
     2161 agtgtccaga tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt
     2221 aacctagact aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc
     2281 cttttggaag ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa
     2341 gttcttttcc tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt
     2401 ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt
     2461 ttgagtttcc acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc
     2521 cagtggatgg aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag
     2581 taatcacttc ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa
     2641 aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga
     2701 cgattttcat catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc
     2761 cattatctgt atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat
     2821 ttggatagag gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc
     2881 caagtgcatc ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac
     2941 gagtcaaatc aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt
     3001 ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac
     3061 catctggaaa agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag
     3121 atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa
     3181 aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc
     3241 cgaccgccct tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa
     3301 aagtttcagc ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca
     3361 tcaatcaatt atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa
     3421 cttatttttt ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga
     3481 catggatgtg ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca
     3541 tcatattttc taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca
     3601 taatgctctc tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt
     3661 gtcttgggta ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa
     3721 atagagaccg tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc
     3781 gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc
     3841 gctattctca tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct
     3901 ttcaagatga ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca
     3961 ataccgatat ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca
     4021 acaggtcttt gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta
     4081 cgggcatgta cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca
     4141 tccccggtga tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt
     4201 gctgcttctg ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt
     4261 gttacataga ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt
     4321 ccaatcttta cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc
     4381 agttgactaa caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag
     4441 atatggctag attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt
     4501 ccattgactg gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta
     4561 tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg
     4621 atcccgtttt tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc
     4681 agtggacaag gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac
     4741 caccaagaaa taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca
     4801 gctaaacgga caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt
     4861 tgatactggc tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt
     4921 aaagaccctg acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc
     4981 aaagaagatt catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt
     5041 tcgccaggct tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc
     5101 aattttcctt ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt
     5161 tctttacccg ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc
     5221 aatatcatca aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc
     5281 gctaatacat ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc
     5341 aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga
     5401 aaaacaaatt cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg
     5461 aaatgtgtca ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc
     5521 cagctgcctc tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct
     5581 taattgaaaa caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa
     5641 tattcagcaa ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact
     5701 atgctatact tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa
     5761 caactagaac aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg
     5821 ctggatacaa aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct
     5881 aatcacaaga ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc
     5941 caacatgagc aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg
     6001 caaacgttgt ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac
     6061 cgtgaaacag aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca
     6121 atcgttattg actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc
     6181 gcattttatg aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt
     6241 aaaatccaag aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac
     6301 tttttgctag agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg
     6361 atacaaaaag cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa
     6421 ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat
     6481 cataatcgca agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt
     6541 tctgattaca tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca
     6601 ttcaaaaatt atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa
     6661 gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc
     6721 ttagtcgatc agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt
     6781 gatatgaaaa taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa
     6841 gaaacaaatg aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg
     6901 aaaaaattag gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg
     6961 gaagaacagc tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag
     7021 gagcgttcat tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc
     7081 catcgaggga tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta
     7141 caaactgtgc cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa
     7201 catttgacaa ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta
     7261 ctattctttt tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat
     7321 atctttattg atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg
     7381 ttcccaaaag caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa
     7441 acgatcgttg gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta
     7501 acgacaagct atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa
     7561 gaacatcaag tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca
     7621 acagatgata tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc
     7681 cggtattggc gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct
     7741 caactgccag acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg
     7801 aagcgttcca ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt
     7861 ttcttgtggg atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac
     7921 accatgtgta cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct
     7981 ttgttaaaca atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa
     8041 ctgctatcat taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta
     8101 attcaatcat ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt
     8161 aatttgacaa gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca
     8221 aataccttgc tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat
     8281 tcaatgaatt ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc
     8341 tctgatacac gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc
     8401 ttctcatttc tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt
     8461 agaaagaaag cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga
     8521 cgaaaaagca gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc
     8581 atacactcta aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct
     8641 tcaacgccga atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct
     8701 aactcttcta ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt
     8761 gcttccatat tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta
     8821 tgctctcatc ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga
     8881 caatctgtta gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact
     8941 ggttgcttag tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc
     9001 cagtgcatca atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt
     9061 ttgagggatc atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc
     9121 ttcttgtata ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc
     9181 cattacatcg cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc
     9241 ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac
     9301 acccatgttc ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac
     9361 attgttcgtc aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg
     9421 caattcagag tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat
     9481 atgttttggt acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc
     9541 atgcggatca ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata
     9601 aatccggcta ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg
     9661 tcccatattt tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc
     9721 cattatactt gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc
     9781 atttttagtc aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat
     9841 tgcctcccaa ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt
     9901 tttttccgat tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag
     9961 catcggtaaa tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct
    10021 ttttgatatt cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt
    10081 ttgtttttac ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca
    10141 tgcgcgattc tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag
    10201 ataacaaccg ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg
    10261 aaagatcttc ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg
    10321 catggcgaat ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc
    10381 ctgcactttt acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct
    10441 ttgcataact taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat
    10501 acagactgcg aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt
    10561 cttgaaatgc ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt
    10621 aatcaaacga agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca
    10681 agatttttac tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa
    10741 gctccactac taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga
    10801 tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg
    10861 ctcctgttat ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt
    10921 actctacgat aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa
    10981 gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta
    11041 ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc
    11101 gttcggatat ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa
    11161 accgctagtc tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt
    11221 ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat
    11281 aactttccaa tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag
    11341 acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc
    11401 ctggataact atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg
    11461 gaaaaacctc atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt
    11521 ctaacgcttg atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc
    11581 acagcgaatc agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca
    11641 tattgccttc cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca
    11701 tcaacatctc ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa
    11761 ccgctaatgc tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat
    11821 gacgtttcga aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa
    11881 tgaaagcaaa tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg
    11941 gagcatctga ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat
    12001 gtacgatcac atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac
    12061 gttttacttc atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga
    12121 tttgacgttc gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc
    12181 gaacgtctaa agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt
    12241 ttaattgttc tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc
    12301 tacccagtaa ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa
    12361 atgggccaat gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat
    12421 ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata
    12481 aatgattttg tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg
    12541 aagaatctct gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc
    12601 atgccagatt gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac
    12661 cattttgaac tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact
    12721 agctttttca ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc
    12781 aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc
    12841 tctagaataa gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa
    12901 tataatcaat tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt
    12961 gacgtatttt cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg
    13021 gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct
    13081 catcgatttt ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat
    13141 aatctgtatc cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc
    13201 caccacgagc taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt
    13261 ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct
    13321 gataagaagg catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca
    13381 tcactaaatg tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt
    13441 tcttctctaa gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag
    13501 cttgcgtatt catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat
    13561 acttttcttt tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt
    13621 cgcaatctat ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt
    13681 gaagtaacga tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca
    13741 ttgctgaatt gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc
    13801 tacgtaaaaa acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca
    13861 atgctttgcg tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg
    13921 ctgttacagt gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa
    13981 actcaaaacg aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata
    14041 atccacctac atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat
    14101 ttacatcatt tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc
    14161 ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga
    14221 tttcttttaa cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat
    14281 ggcgatggat aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga
    14341 ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg
    14401 tatttttgat gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat
    14461 agacatcccg tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag
    14521 atttcttagc actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt
    14581 ccttcatcca ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt
    14641 tcccaactaa aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac
    14701 cattgaagtc aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat
    14761 tcagtaagcg caaaaagtcg tcatccgtca atgagacatc ttggatctca ttataatggc
    14821 gtaatctgaa acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg
    14881 tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga
    14941 tacattgtaa ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc
    15001 taatagatag gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc
    15061 tgttgcgata atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat
    15121 atcagtcata atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt
    15181 ctttgctatg ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc
    15241 aaatttttca atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg
    15301 gattcctgca tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt
    15361 attccatgca atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt
    15421 taattgggca ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga
    15481 aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc
    15541 gtattatact gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa
    15601 gcatttcttt acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa
    15661 aacaactaat attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat
    15721 cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat
    15781 cgtggcaact gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg
    15841 cgtagttctg gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct
    15901 ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc
    15961 atccagactt catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg
    16021 atgatggcga tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc
    16081 tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag
    16141 gtaatcaccg gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag
    16201 ccttcaggat ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt
    16261 tgaataccga tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct
    16321 tcgtcatctg caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca
    16381 aaaaaatcca agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct
    16441 gtcttatcca cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt
    16501 ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc
    16561 agccttttgg aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc
    16621 agcaaaaccg atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact
    16681 ggcagttact cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt
    16741 gctatgccgt ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc
    16801 ttgcatccgt gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc
    16861 tcgttcgaat aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa
    16921 attaggatcc atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag
    16981 gactgcttca tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg
    17041 aaatgctagc ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc
    17101 aataatcaaa gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca
    17161 tactttttct tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc
    17221 ccacatgtta tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat
    17281 ccgaatatat ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg
    17341 tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac
    17401 tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat
    17461 aaattcactt aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac
    17521 gattattttt gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc
    17581 actgtctact cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg
    17641 agaaggagca aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc
    17701 ttggaatact acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg
    17761 tttttttact aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg
    17821 agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc
    17881 ggcattttca taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct
    17941 ttttgtttgt gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg
    18001 taaagaacaa tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg
    18061 aacttctata tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa
    18121 tgccgctgct gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg
    18181 aactttccga atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc
    18241 aattctgtct gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg
    18301 aatgactggg acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc
    18361 atcgatcgtt gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc
    18421 aaatcggcta ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac
    18481 gattgcagcg ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc
    18541 aatgcagatt gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata
    18601 tatcgctacg gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc
    18661 accgcggtta gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta
    18721 attccgcttc ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg
    18781 aagctttcac tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct
    18841 tacggaattt tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa
    18901 gagaaagtaa tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca
    18961 taacaggttc ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct
    19021 tagcaacgat ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat
    19081 aacgatgtgg aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct
    19141 ttgcttattt ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt
    19201 ctacaaggat ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca
    19261 tcgcttcaat aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag
    19321 ctggtttgtc gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga
    19381 ttccatcaaa tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt
    19441 cagatatgac attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca
    19501 tgcctcgact tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga
    19561 attccgttag cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca
    19621 aaattattcc tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga
    19681 attactcatt gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc
    19741 aatctcagga tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc
    19801 cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga
    19861 tttcgtgctg ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt
    19921 tgcttctgcc tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg
    19981 ttccggctgt acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc
    20041 atctggagta ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc
    20101 tccatagatt ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc
    20161 tgctccttca cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt
    20221 aggatcagtt gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca
    20281 aatcgttgct tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg
    20341 gaatgcttcc ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat
    20401 accttttgca cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg
    20461 tacgaacatt ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc
    20521 gattgttgga agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat
    20581 tttttcttta tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat
    20641 cgaaaacata tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc
    20701 tttgacttca cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc
    20761 gatgccattc gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg
    20821 tgaagtgact ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta
    20881 tccgtgcatg accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg
    20941 tgctaagaaa actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat
    21001 ttgcttttcc gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat
    21061 gaatcctggc gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga
    21121 ttttgttaat cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc
    21181 aatcaaccca gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt
    21241 tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt
    21301 gaaatcttct gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac
    21361 taacacatgg atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc
    21421 ataatcagag atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc
    21481 tatcttttct tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc
    21541 aaaagctaag gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt
    21601 tccttttaca tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc
    21661 ttattctcaa cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat
    21721 acttttccgg ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata
    21781 ctctcataga aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct
    21841 ttcttcatta tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga
    21901 atctgactta ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa
    21961 ggcccgctga catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca
    22021 gcttcgtcta ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt
    22081 gcaggagata cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc
    22141 ggcgcattca tcactgcaac cattttcccg cttccagctg gggtagcttc tgtcatataa
    22201 gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact
    22261 aatgccgaat attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc
    22321 ttcaataagc gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc
    22381 tgatccaaac gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca
    22441 cttgcttcat caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct
    22501 tgatattgtg caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata
    22561 tccagaatat atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa
    22621 atgtcccagc ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac
    22681 taaatcttga accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc
    22741 aatttcattg atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc
    22801 agtagtcaat ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat
    22861 tttttcgaat accataataa tatattcctc cgatattgtt tgctttttat ttttataatt
    22921 taacagtcta caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa
    22981 atattacttt ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata
    23041 agatagggat gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt
    23101 cttgcggcat ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca
    23161 aaagcagata atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc
    23221 gtacggcaaa atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat
    23281 cttctgaata gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg
    23341 taccgtcagc tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa
    23401 tcccacctgc tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct
    23461 tagatagtgt ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt
    23521 cagccatgct taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag
    23581 catgagttgc tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg
    23641 gcgacattgt tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta
    23701 tcaatttttc cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc
    23761 gttcacgaat acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa
    23821 gatcattatt agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa
    23881 attgttccat taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg
    23941 catccagcaa ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt
    24001 cacgatgaaa atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact
    24061 ttacaacgcg gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact
    24121 tgttaatagc agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag
    24181 ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat
    24241 ctttgaattg agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac
    24301 taacaagata atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta
    24361 gtttgattat caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc
    24421 ttaaaagatc atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac
    24481 tgataaatca gcatttataa tttcttttat tagaaaaatc atctatctca catattttga
    24541 ttatcaaagt attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc
    24601 aaaatttgtt atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa
    24661 atgaaaactc tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc
    24721 atctatgttg gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat
    24781 aaacagcatt caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc
    24841 gtagtagaca cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt
    24901 cctttttata accgacaaat gaattatttc agtaattata attatgtaca tattaccaat
    24961 aagggatttg tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat
    25021 aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct
    25081 gaaattagtt tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa
    25141 cagaaaaaaa aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac
    25201 ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt
    25261 caaaaagata tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt
    25321 ttaagaacac catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag
    25381 cactacctcg tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct
    25441 cctgacttct attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa
    25501 gaagaaatcg atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag
    25561 tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc
    25621 ggcttctttt aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag
    25681 tcatagattt ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct
    25741 gtttcagtta attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg
    25801 ccttcttttt caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc
    25861 agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt
    25921 tctgttatat tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt
    25981 tttttatgat tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg
    26041 ggttccgtca ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt
    26101 agttgctggc aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt
    26161 gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata
    26221 attgcttaaa gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa
    26281 atcaagtccg tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc
    26341 aatcatgatc tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt
    26401 aaatgtcgta ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc
    26461 accgaccatg atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc
    26521 atctaacgca tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg
    26581 tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga
    26641 ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt
    26701 actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg
    26761 atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgttt ctagtagatg
    26821 gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac
    26881 ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt
    26941 cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa
    27001 tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat
    27061 aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta
    27121 aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt
    27181 cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag
    27241 catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt
    27301 gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat
    27361 cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca
    27421 attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat
    27481 aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa
    27541 tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt
    27601 tgctgatatc ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag
    27661 gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac
    27721 ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg
    27781 attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt
    27841 aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc
    27901 cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat
    27961 accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca
    28021 ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga
    28081 cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc
    28141 tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac
    28201 tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt
    28261 tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga
    28321 taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata
    28381 aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac
    28441 actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc
    28501 ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct
    28561 atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca
    28621 attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg
    28681 tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac
    28741 aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt
    28801 tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga
    28861 atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc
    28921 agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg
    28981 ttcgatccga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact
    29041 ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg
    29101 atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc
    29161 agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca
    29221 ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata gtataacata
    29281 gcctacaaac tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg
    29341 ttttcttaaa aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg
    29401 atacgttgtt tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca
    29461 ttctttcaat gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa
    29521 actacgcgtt ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac
    29581 tagcaatgga acagctgttt gatctccagc catttctcca cacatacctg cccattttcc
    29641 ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg
    29701 ttggtacaag taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa
    29761 atcgttcgta cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc
    29821 tgctgctgga atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc
    29881 acttatcaat ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag
    29941 tgtagctacc atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc
    30001 acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg
    30061 atatcctaag aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc
    30121 accgatgtcc attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt
    30181 gtaagcttca tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc
    30241 agtacggtac agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg
    30301 tgttccgatg tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt
    30361 ttttaatttt tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc
    30421 agctttttgt tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa
    30481 aatatcgcct tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc
    30541 taaagaacgt gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc
    30601 ttttacaaaa ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac
    30661 gaccacttct tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa
    30721 aatacgttta gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc
    30781 cattgcttcg aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt
    30841 tacgctgttg tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac
    30901 catcaaatgg gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc
    30961 acgaatttgc tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc
    31021 agccgcagta tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta
    31081 agcttttgca acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat
    31141 tcagataagc cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca
    31201 gcaccttctg cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc
    31261 atgattgatt tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat
    31321 ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact
    31381 acgtgaaatt ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg
    31441 tgagggtttt cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta
    31501 aagattaaca ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat
    31561 tcaaattatt ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat
    31621 ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct
    31681 ttgttttcat atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca
    31741 aaggtcagga atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac
    31801 taggggttat tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt
    31861 gtgaaaggac gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt
    31921 cacttgtatg ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat
    31981 caaaaaatca aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt
    32041 ggatttggca gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga
    32101 aattatgagc aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag
    32161 ccacctcaag gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa
    32221 gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt
    32281 gaaattttaa atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt
    32341 aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa
    32401 ttaatggaca aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata
    32461 cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat
    32521 gtgatcctat tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga
    32581 aatatggatg ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt
    32641 ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt
    32701 atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt
    32761 ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca
    32821 gccgcaactt tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat
    32881 ttgctagatg aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca
    32941 atcgagaaaa aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat
    33001 ttcgaaaaag cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt
    33061 cagctcactg aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa
    33121 caacggacag gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac
    33181 ttagcagatg acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg
    33241 aaagcgattc gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc
    33301 ttatttgttg gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa
    33361 ctattcggtt cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat
    33421 agtgtctcta aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa
    33481 ttaacagaaa aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa
    33541 gctcatccag atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat
    33601 gcacaaggaa gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga
    33661 actggtaaag tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct
    33721 gtattgaacc aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc
    33781 atcgaattca gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac
    33841 gatgtcaacc aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa
    33901 gaaaaattag ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg
    33961 atccaagaac aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat
    34021 gagttgaaag caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt
    34081 ttggctaaag aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg
    34141 tatttctttg agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt
    34201 tataaaaaag aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc
    34261 gccatgaaat tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa
    34321 aacactgatg cccatctagt caaggtctac acagccggaa ataccactat tgtctacact
    34381 gaagcccctg aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa
    34441 gaaatcgaag atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag
    34501 gatgaaatca aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa
    34561 ttaggcgcta cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac
    34621 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc
    34681 aactccttcg gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc
    34741 agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga
    34801 tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt
    34861 tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt
    34921 gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt
    34981 ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat
    35041 atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt ttaatatagt
    35101 aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga ggctgttttt
    35161 gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct ttacatcaaa
    35221 ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa ttggttctcg
    35281 aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact tgaactcatc
    35341 ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat tggagacatg
    35401 attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa ttgtagctga
    35461 ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg aatcgctcct
    35521 tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac attttttgca
    35581 gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc aacttttagt
    35641 tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac accgatgata
    35701 tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg tgtgacattg
    35761 cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt
    35821 ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat atttgcttgg
    35881 atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg atcatcttgt
    35941 gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca
    36001 ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg agcaattttt
    36061 tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa gacttgttca
    36121 tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc atccaattct
    36181 actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt atggtagata
    36241 tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg ataagattcg
    36301 atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc atcacgatct
    36361 agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg gaataatttc
    36421 tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact atccacggcc
    36481 atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt
    36541 actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat cccacgttgt
    36601 ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc ttttacggtc
    36661 ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt tgttttccca
    36721 gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt
    36781 ggattattca taacctaact ccatttcttc cattcaatta tttatatttt ctctaggcac
    36841 agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa aagccgtgta
    36901 gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa taagcccacc
    36961 caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta atgcaggata
    37021 gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg attcagcgaa
    37081 tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact aaaagaaaag
    37141 acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca attatcttct
    37201 tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag gttccgcttc
    37261 tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac cttccatttc
    37321 ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt catccggaat
    37381 catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca tatgcagatc
    37441 actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga tatattcgtg
    37501 ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga tttctccgac
    37561 gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat attcatctaa
    37621 taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat caataaagac
    37681 cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat tatcaaagcc
    37741 gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta ctttatcttt
    37801 gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat tcacattttc
    37861 ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca tgacttctcg
    37921 ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt cattattcaa
    37981 tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg aatcttcatc
    38041 atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa gcagccagac
    38101 gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact ttgcgacttc
    38161 ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa tcgatacata
    38221 ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc cgccacctaa
    38281 taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca aaatgttgac
    38341 caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca atatttttaa
    38401 aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat tcttattgat
    38461 cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca aaacgatcaa
    38521 taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa tcgcattctc
    38581 tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat agccacgtta
    38641 aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa actacctacg
    38701 aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg attttgatta
    38761 tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag ataccaagca
    38821 atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga tggattttca
    38881 gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg acgattagca
    38941 caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact agaaatgcag
    39001 gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc
    39061 caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg aaggtaccaa
    39121 cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta ttcagttgcc
    39181 ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg atgtttggca
    39241 ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta atgattacta
    39301 cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact ccactaagga
    39361 attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta ttgatatcta
    39421 cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc tgtttgatga
    39481 aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg atgacactaa
    39541 gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca gcccatattc
    39601 ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag taaagaaagc
    39661 ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa
    39721 agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca agcgaccaaa
    39781 acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta
    39841 gtatatatta tattcgttaa aaattctttt attctttttt tatttttgaa aacttgccat
    39901 actttttatt tgttttattt tccagtatac tatacgaaag gaaggtggct agaccatgaa
    39961 agttgtacaa acaaaagata caatgagcaa tatttactta gatgctgtac ggatcaggca
    40021 tcaagttttt gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca aagatgaagc
    40081 ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct
    40141 tttgccttta gaaaacggaa aaatgaaatt acagcggatg gcgattcttt ctgaatacag
    40201 acaccaaggg ttaggaaaaa tattgattga agaagcagaa aactttgcta agaatcaagg
    40261 ctataatacg attttattag gggcacagtc cacagctgaa actttttacg aaaaactagg
    40321 atatacagct tacggcgatc cttttgaaga tgctggcatg cctcatattt atatgaaaaa
    40381 gactttataa aaatttattt cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc
    40441 tcactggctt actgttcttt tttgtgttta cttacgcgac aattgcttgt aacggttata
    40501 ccaaatatca atatagcctt ccgagaacgg gccatctcca ttattgatcc agtcaaccag
    40561 aatcttcaca ttttctttta ggatgaagtc aatatcgttc gggtaattca tcattttgct
    40621 gtgcatctca tattcatcga catccaatag tcgtttctcg ccgtcaggaa ataccttgat
    40681 atccaagtca tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag
    40741 attacaataa taggaaaccc ccttttctct gatcattgct attacattga accagtattt
    40801 tttatgaaaa taaacaatcg ctggttcgcg cgttacccat cggcgtccat cagattcagt
    40861 gactaatgtg tgatcattca cgccgatcat agagtgttcg cttgttttta ataccatggt
    40921 atctcgccac gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc
    40981 tccttctttt ggaactccca tagcaaccct tccttaagca gcattttgtg ctttgtgttt
    41041 gggtgttcat tacaccgaca tcataattat tatatcaata aaaatgagct ttgcctagaa
    41101 aggattccta ttgtttcgat ttttctctaa ctgatctacc agtttcattt ggaccttagg
    41161 aaaaacgacc gaatcaaatg catcaggctt caaccacttc gaagttttgt tctcggtaaa
    41221 ttcttgttct gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg
    41281 actaaaaata tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt
    41341 ttcaaatggg agattttgct cttcttctgc tactaggtca tcaaacagat cgatttctgg
    41401 ctttgtttcc cattcttttt tcaatcgctc atactcctct tgtgtcactt ccatcattgg
    41461 aaatgtccac atattcgcca gcaatttttg gctatctctt tcttcaaaat aatacgctcc
    41521 agaatgattt tgaagagctg cactgatata ataaacgtct tttggctttg ctttttttgt
    41581 ttttacagga aaactcgttt ggattcctcg tttgttagct aaacaaaagg cttggatcgg
    41641 acaagtctca cactttggag aagttggtgt acagatagct gaacctagat ccatcattgc
    41701 ttggttgaat tcacctggat gcttttcatc aatgattttc cgcatcgctt catcaaagat
    41761 tttcctgcta gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg
    41821 catcacgttg ccgtcaactg caggttctgg aagtccaaat gcgatacttg caatcgcgcc
    41881 tgtcgtatat ggtcctattc ccttcaatga gctaatttct tcaggcgttt gcggcatttc
    41941 cccatcaaat tctgacatga tctgtttagc agcagcttgg atgttccggg ctcttgagta
    42001 ataaccaagg ccctcccatg cttttaaaag cttttcttct ggagcatttg ctagctcttc
    42061 aattgtagga aaccattcca tgaaacgata aaaataatcg atcactgtgt ctactctggt
    42121 ttgctgaagc atgatctcag agatccagat acgataagga tcacgattat agcgccaagg
    42181 caggtttctt ttttcttgtt cataccattg aatgaactga tcttgaaatt cttttgtttc
    42241 tttatctgtc catttttccc attctttatt cactttcctc ttccttttcc gtaatgaatc
    42301 gctggatcat atccaaatca aatccttttt gatataagct ttgctttact tttagatttc
    42361 tttttttagg ttcaaacctt aaatttcttc gccatagttt atctccttgt ttgactaaat
    42421 aatcatattc tatttcttga tctgcttctt gcggaagatc agctatcgct tgttggatca
    42481 cttcttgaga aaagcctttc gtcatcaatg tttgttggat ttttctcaat aattcttttt
    42541 ggctttttcc gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt
    42601 ggtcttcaaa tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt
    42661 gctgcatctg ctgcgctaat ttctttggtc ctttgtcact taagcgcata ttcgtgcgca
    42721 cataactttc cccatagacc aaatcatcga caaggtccaa ttcttttaag cgggctacga
    42781 ttttatttcg atcttcttga aggatttcct tttcttttaa gtatgttcgg atttcttttt
    42841 ccgatcgcaa ctgataactt aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc
    42901 tactcttctt gatctcttgg atagtttctt cgtctaattc ccgtcctttt aataatcgat
    42961 agcgtactaa tacatcttca gatacttgta aagcttctcc ttcagataaa tctacgcgat
    43021 agaaagggcc tttgccttta ctgattctcg taacggttaa cataaacatc ctcctcgaac
    43081 atatattctc tatcatacca cagaaaatcc ccgtaatcag gtattatatg ttagaataaa
    43141 ccacagaaaa aagaaaaagg gagatactta tggcagaaac aattgtcaaa actggacaaa
    43201 cggttccttt gaaaatcaag cgtttaggca taaatggtga aggaatcggt tatttcaaac
    43261 ggttgatcat atttgttcca tatgcactac caaaagaaga agtactagtg aaaatcacga
    43321 aagcaactcc tcgctatgca gaaggagaat taataaaagt caaaaaaaca agcaaagatc
    43381 gcgtagtggc accatgtcct gtttactatg aatgcggtgg ctgtcaatta caacacttag
    43441 cttatcaagc tcaattggat tttaaaaagg atctgctttt gcaagcattg gaaaaattca
    43501 aaccagcagg ctttcgatct tatcaacttt tgccgacgat cgggatggat gagccttggc
    43561 attatagaaa caaagcacaa tttcagttac gaaaaaataa gcagactcaa aaaattgaag
    43621 ctggtttata cgaagcaaac tctcatcagt tagtccctat aacagactgc ttggttcaag
    43681 aacctcgaac acaaaaagta atgaatactg ttgttcaact attgaataaa tttgatgcac
    43741 ccatctatga tgaacggaca aacagtggaa tcttccgcac aatcatggtt agaatcggtg
    43801 ttaaaactgg ggagttacaa gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa
    43861 acgcaatgat ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg
    43921 tccaggcaca aaaaacgtct ctcgtgatgg gggatgaaac gatccactta tggggaaaag
    43981 aagccattga agaacaaatc aatgaagtga ctttcgatct ttctcctcgt gccttctttc
    44041 aactcaatcc tcagcagacc gaattgcttt atggcgaagg aatcaaagca ttagatgtcc
    44101 agcctaatga aacgatcgtg gatgcttatt gcggagttgg gacgatcggc ttaagtgtag
    44161 caaaacaagc caaagaagta cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa
    44221 aaaaaaatgc ggaaagatta ggatatcaca acactcgata tgaagtcggg acagcagagc
    44281 aagttctgcc taaatggttg aatgaaggtt ttcagcctga tggtatcatc gtagatccac
    44341 cacgtacggg tttggataac gcattgatcc aatcattatt gaaataccct ccaaaaaaac
    44401 ttgtttatat ctcctgtaat gtttctacac ttgccagaga tctggttaat ttaagtaaaa
    44461 agttcaagat tgaatattta cagtccgtcg acatgtttcc tcagactgca cgttgtgaag
    44521 ttgtcgtgaa gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc
    44581 tgtatcaagt aataagaaca gccaaacaaa aatagctctt catgttttat gaatactgta
    44641 cttgtgaccg aagagttaac aggaggacac tatttattta aaaaaagggt tgtgacatag
    44701 tttttgtcac agcccttttt gattattatt ggatattcac gtctatcact tgataaaatg
    44761 catttgtcgt atcgctgata ttccatactg ctaaaatcac attatatcct tttcgatcct
    44821 gcggaagtgt gatcgtttgg ttgactgttt ttggtggaac agttgctttg tcgtctattt
    44881 ttgtgatcaa ttcaaaatca ctaaatttca aaggttgatt aggattccaa gttggtttgg
    44941 taatgtagta atcccaagta cttgtccgat gttgtgctgt taaattccaa gtaatattta
    45001 atgttcctgg attaaccatg tttttatgcc atctgttaga tgtctgttca tctaaaggct
    45061 caaagccgct gactcctgca ctagcaattt ttccatcaat gaatgtattt ttggtagctt
    45121 cgatactttg cggttcccat tgtgctcttc cgacattttg atttaagttt cctgctgctg
    45181 atgttcctag gtatgcacgg cttcctggag aagtaatata accatgcgca aaacctggaa
    45241 tgctaaaacc gaacaaccct aaactaacta acccaataaa caataacgta ctaatctttt
    45301 tcatgtagaa acctccttca tttgataacc acattgtaaa cgctttattg tattaacaca
    45361 aacattatgc ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga
    45421 ttataacaga agtagacgag aaaagtacaa atagaaaaca tgagaatcaa atcatatgat
    45481 ctttttcaaa aacatcattc atataaacaa atgttcaact ttttaataac aacaattatc
    45541 aatttttttc ttacttcaca aactaaatac aggtcttgct gtgagccttc atggtaaact
    45601 aatattatag aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg
    45661 tgttatagga attgtcatat taggcttttt atcagggttg cttagcggaa atcccggaaa
    45721 atattactat tctttgcaac ttccaccatt tgcaccacct agctggatat ttgggccgat
    45781 gtggacctta ctgtatatat tgatgggtat ttctctttat ttgttactta atcacaacaa
    45841 caaaaaacag cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt ttaattttat
    45901 atggtctgct ctatttttta atttaagaaa tattttcatt gcagcaatcg atattacact
    45961 tttagttatt ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat
    46021 gtggctcatg attccttatt atttatgggt actattcgca actctattga attattcaat
    46081 ttatttttta aactaagcgc taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg
    46141 ccacctaagt tagatttttt gtctaattta ggtggcactc tttattaacc aaactatttt
    46201 tttctagtac ccacttgttg ttccatactc attattctgg tcataaccat aaccattttg
    46261 ttcctgtcca gcgtaaggat cataactttg agtatcttga cttggtgtat agtactgact
    46321 tgtatcttct gtttgctgat aataggtatt cggatcttgg tactgatcat atgtgttttc
    46381 tgaataggaa gaataatcgg tgtacccttc actattcgat ccatcaccgt ttgtcgaatc
    46441 gccatcttcc gaatcatcaa agaacatcat tgaagcattc tcatttttca aattaggtaa
    46501 tgtatcgctt gtttttatat ttaattgttt cttcagttca ttttggatcg aaagaagatc
    46561 attttttcct aaaatttgat agtagatatc attgatcatt tgtccttcgc cttgcaactg
    46621 gtcttgtttg attgtttcaa acgccggggt gtagttcgtc gctacatcaa gcatgtcatc
    46681 ccaatctaag tccgtcgtca cattgttctc aactgctttt aaaatttttc tataattgct
    46741 tacgccatct aaacttaaaa ctttattgac gattttcgtc actacttccc gctgacgacg
    46801 ttgtcgaccg atatcgcctt ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt
    46861 ttctccattc aatgtttgtt cacctactgg tacatggaca ccatccaaag taaagtcaat
    46921 tttattattc actttgatgc caccgaccgc attgatcaaa tcttccatgc catccaaatt
    46981 gatcgttaca taatgatcaa taggaatatc gagcagttgc tcaattgaat ccatggccat
    47041 ttcgacacca ccaaatgcgt aggcatgatt taatttatct accgttccat aacctacgat
    47101 attcgtataa atatctcggt ccaaactgat gatcgttgat tttttttgtt tcggattaac
    47161 tgttactacc atcatcgtat cagaacgtcc ttgttccgtt cttcctaatc caccggtatc
    47221 taaacctaat agcaaaaccg aaaaaggttc tttgtcatca atgcttactc tctcagaacg
    47281 tttggaggat attcgattag agtttttaga aattttgttt actgtttggc tcgcttctcc
    47341 cataaatctg attccgtatg ctgataatcc ggcaatcagc aaaacgaata cccctaacaa
    47401 ggttaataga actttttgcc atcgcttcat aatacacctc tgtttcacat tttctaaaat
    47461 attgtctcat ataactgttc aatcagcaaa ttcacttttt aatcataaga aaactttaaa
    47521 aaacattttt gtgtaacgat tattattttt taaaatttct caaaattctt aactatcata
    47581 ccataccgtg aaaaagatcg aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt
    47641 tttatgtatt ttttccagaa aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta
    47701 aaatgatcgc acataccttt cccatctttt taacagtctt tgcctaccgt tcagattatc
    47761 attcgatttc acgacaattt cttcctatga ctggtccttt tgttaaaccc atcttctatt
    47821 cgttttattt ccagctcact gcatggctct taaaatgact cgaactagtg attgcattac
    47881 catcatcttt taatggatag atatgctcct cttcaaagta aaacgatttt cttttaaagt
    47941 aatagaattg acatggtaat gagtatgtat actagtggtt tcacaaatgg tcttttattc
    48001 aacatccaaa aactgaaatt tctttaaaat ccattactaa cgaagaaaat gttccttgct
    48061 tgataattag ttaggacatg tcaagtgaac aaatgcattt acttagccaa aaaatctaaa
    48121 aacttcataa atagacaaaa agacacgact ctctaagaag tcgtgtcttt attcaacaaa
    48181 ttattataat ttagtcactt cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa
    48241 ttctacttct tgaccttctt ctaggctctt gaagccgtct ccttgaattg ctgagaaatg
    48301 aacaaatacg tcatctccgc cgtctactgt aataaaccca aatccttttt cattgttgaa
    48361 ccatttaacc gttccgtgtt ccataaatgt atatcctcca aaaaagttct tgcaagtaac
    48421 cttgcaagaa cattataccc aatttagaaa acgctagcaa gctttcgctc ttttattttc
    48481 caaattattt ttaagactga tatgacggag gaaatggtta ttttgctgct tcgaaacgtt
    48541 tgtttacttc atcccaatta ataacattcc agaatgctgc aatataatct ggtcggacat
    48601 ttttatattt caaataataa gcatgttccc aaacgtccaa acctagaatt ggtgtctttc
    48661 cttccatcaa tggagaatct tgatttgcag tagaggtaat agctaatttt ccattttcca
    48721 ttacaagcca agcccatcca gaaccaaatc gtccagcggc tgcttttttg aattcttctt
    48781 taaaagaaga aaaatcacca aaagcttcat taatcgcttc ttttatttct cctgtaggtt
    48841 cgccaccagc atttggtgcc atgatttccc agaaaaatga atggttagca tgtccgccac
    48901 cattgttacg tacagctgtc ttgatatctg ttggaatagc gtccatatca gataataatt
    48961 cttctattgt tttttcgcct aattctgggt atttctcaat tgctgaattt aaattcgtca
    49021 cataagtatt gtgatgctta tcatgatgca gatgcatcgt ttcttcgtca atataaggtt
    49081 ccaatgcgtc atatgcataa ggtagatctg gtaatgtgta agtcataaaa aaattcctcc
    49141 tcaatgtttt cttaataatg tctacattac aaagagtaac aaagagaagg aaattcttca
    49201 attgttatgc ctagtcgtct ttttcacttt gacctacttc ttcttcaaaa acctcagttt
    49261 ctttaaaaat aaacaacttg ctgaaaatat agttcgcaag gataacaata atttgagtga
    49321 tgattttcgc cacatactca ttgctgttca taacattgat caacaatacc attgccccca
    49381 tgtccaatcc gtaagaaagg atacggtaga agaagaactt accgcattca ataagtgcat
    49441 gtgtaaaatt cggtgatttt gaatggaata cccaaagttt gttcgttaca aaagcaaaca
    49501 atacagaaca aaaccacgaa atagaattgg ataaggccat acttaaatca aaatattggc
    49561 gtgcgagagc aaaactaaca aaatttacaa tcgtagccaa accaccaaaa aagagataga
    49621 ctagtatctc ccaatatcct tttgcttcta aatatccttt taactgtcga tactgcttca
    49681 tgagccaacc taccttatta aaaattctta tttatcatac tctatatttc aataactttc
    49741 atctttattt aaaaaaatta attttttttg atcctttgcc tttgtctctg cttggatcgt
    49801 cacatgtgta ataccatatt ttaattttaa taaagaaatt atacgaggat atatcttttc
    49861 aatctcgctt agtagctgat cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc
    49921 gctatactgc caaatatgga catgatgaac atcctttacc ccatcgattg cttcaatttc
    49981 ttgttccatt ttttcatagt cgatagcagg tgcactttgt atcaaaatag cggcagcttt
    50041 tttgatcacc attatcgctt ctatcaatat atatatcgaa atgatcagtg taataactgg
    50101 atcgatccag tagatatcga aaaaacggat caaaattgct ccgataatca cacttaccga
    50161 agacagtgta tcacttaaca aatgcaaata actagatttt atattcaaac tttcatctgc
    50221 ttcttgtcgt aataataata ctgagaacaa attcgcgagc aatccaataa ctgctactgt
    50281 tagcatcaaa tgactattta tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt
    50341 taaaaacaag gataatatga tcaatatgaa tgagttgatg aaagcactca atatttctaa
    50401 acgctggtag ccatacgtac gttttttatt tgcttctttt cgggccattt tttgtgcccc
    50461 gtaagaaaaa ataattgcca atgtatcact aaggttatgg aaagcatctg aaacaagaga
    50521 caaactccct gacataatcc ctccaataaa ttctgataaa gtgatcaccg cattcaaaac
    50581 cactactcca aatagttttt ttgatgatct ctgtggctgt gtaggcacta ttccttcctc
    50641 cattttattt atattggata gtctttagac aagaataaca tttttttgat atttcaccaa
    50701 tcattcagct gtttccttaa agcacttgta tttcacttga tacttcgcta gaattgcaag
    50761 tgatgacact actagatgag gagtacttat gacaacaaaa caattaatta tcagttcctt
    50821 tatacgattc aaagacctaa aggaggcgag aaatgtccca ttttggaaag ccattgtcta
    50881 tcttttagtt ttgagtataa ttatggcttt gccaatctct tttcaagttt ttcaagtctt
    50941 aaacaacatt caattggatg ggcaaaagat tgctactcgc attcctgatt ttacaatcca
    51001 aaacgggcag cttcaaaccg aagaaaaaga aggattcatt taccaaacaa actccattat
    51061 cttcacattt gatccagagg gaaaacgaac agaacaggac atctctactg atttgatggg
    51121 gaattttgtt agtgtcggga tgttaaaaaa caaacttatc atagctcttc ctaatacagg
    51181 cacgacttct gctttgttga acaataacca attagaactt ccctacacca atgaatcttt
    51241 aaaaaactta acaggtaagc agctccgttc ttttttaagt gaagcatcta taccactttg
    51301 gataaaagtc ctgacattta tattttctat ttatcctagt tttctgaatc tgctgatcac
    51361 tttgctattt accaacattg ctgcttttct ctatgcacgc tttcgactaa caaaagctac
    51421 ttttcttgat tgtttaaaaa cattgatcta ttcaatttca ttgcctacta taattgcaac
    51481 aatcttgatg gtctttcttc cttcttttga cactagcgcc ttcattgctt ttgcaggaat
    51541 ctttattttt gctcaagctg tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa
    51601 gacaactcgt tatttctact tcaatagacc cttttagagg ttgtgacatt tttttgtcac
    51661 agcccctttt ttatttgaaa aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt
    51721 tcttcttgta tgcgctacac ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag
    51781 aatcatttat gcagatcgtc catttcgtga tgaatttatc gaagaaatcc atactatagc
    51841 gcctggttat atctttaaaa cagaattaca accagaggat ctacaaaatg tagagatcag
    51901 tttgggctgg aactcagact accaagaaaa actattagcc tcttctacct tgaaatgggt
    51961 ccaatctatc tcagctggtg ttgatcagct accccttgag actttttcaa agaaacatat
    52021 tttactttca aatggtagtg gcatccacag tgaatcgatt acggagcatg tcttaggaat
    52081 catccttggg tattctcgtg gtctttttca agcacaaaag gcacagagtg acatgaaatg
    52141 gttaggttca tcggtccatt accaatctat ccaagggaaa aaaatactaa tcattggaac
    52201 tggccatatt ggaaaaatga ttgcaaaaaa atgcaaagca ttagacatga actgctatgg
    52261 aatcaataca tccggtcatc cagcagaagg aatggaaaag actttttctt taaatcaact
    52321 aaaggaagtt cttcctgaag tagatttcgt agtcaatatc ttacctctaa cggaggaaac
    52381 aactgggctc tttgatcagc atatgtttga acactttgat ccaaaatctg tattcatcaa
    52441 tgttggccgt ggtgcttctg ttaagacaca agatctggtg caggctttaa ataaccagca
    52501 actttccttt gcagcattag acgtttttga ggaagagcct cttccacaag atcatcctct
    52561 ttggaaaatg gacaatgtac tgattacttc ccacattgca ggtttgacac ctgattttca
    52621 aaagaaatta atggctattt ttttaaccaa tctgaaaagc tactttgcca cccatga
//
LOCUS       DAYYGY010000009        51582 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000009 DAYYGY010000000
VERSION     DAYYGY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.009"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHA52_000574"
     CDS             complement(93..1136)
                     /locus_tag="ACHA52_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8939327.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHA52_000575"
     CDS             complement(1513..2478)
                     /locus_tag="ACHA52_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8939328.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHA52_000576"
     CDS             2712..3557
                     /locus_tag="ACHA52_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8939329.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHA52_000577"
     CDS             3748..4935
                     /locus_tag="ACHA52_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8939330.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHA52_000578"
     CDS             4941..5297
                     /locus_tag="ACHA52_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8939331.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHA52_000579"
     CDS             5299..5637
                     /locus_tag="ACHA52_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8939332.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHA52_000580"
     CDS             6032..7066
                     /locus_tag="ACHA52_000580"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8939333.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHA52_000581"
     CDS             7059..7745
                     /locus_tag="ACHA52_000581"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8939334.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHA52_000582"
     CDS             7769..8617
                     /locus_tag="ACHA52_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8939335.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="ACHA52_000583"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="ACHA52_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8939336.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="ACHA52_000584"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="ACHA52_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8939337.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="ACHA52_000585"
     CDS             10883..12118
                     /locus_tag="ACHA52_000585"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8939338.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /gene="sufU"
                     /locus_tag="ACHA52_000586"
     CDS             12105..12575
                     /gene="sufU"
                     /locus_tag="ACHA52_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8939339.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="ACHA52_000587"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="ACHA52_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8939340.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..15211)
                     /locus_tag="ACHA52_000588"
     CDS             complement(14069..15211)
                     /locus_tag="ACHA52_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8939341.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15262..16059)
                     /locus_tag="ACHA52_000589"
     CDS             complement(15262..16059)
                     /locus_tag="ACHA52_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8939342.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            16261..16560
                     /locus_tag="ACHA52_000590"
     CDS             16261..16560
                     /locus_tag="ACHA52_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939343.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            16825..17700
                     /locus_tag="ACHA52_000591"
     CDS             16825..17700
                     /locus_tag="ACHA52_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939344.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            17697..17915
                     /locus_tag="ACHA52_000592"
     CDS             17697..17915
                     /locus_tag="ACHA52_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939345.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            17902..20529
                     /locus_tag="ACHA52_000593"
     CDS             17902..20529
                     /locus_tag="ACHA52_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8939346.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            20544..20762
                     /locus_tag="ACHA52_000594"
     CDS             20544..20762
                     /locus_tag="ACHA52_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939347.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            22281..22595
                     /locus_tag="ACHA52_000595"
     CDS             22281..22595
                     /locus_tag="ACHA52_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939348.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(22648..23421)
                     /locus_tag="ACHA52_000596"
     CDS             complement(22648..23421)
                     /locus_tag="ACHA52_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939349.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            23526..23771
                     /locus_tag="ACHA52_000597"
     CDS             23526..23771
                     /locus_tag="ACHA52_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8939350.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            23927..24778
                     /locus_tag="ACHA52_000598"
     CDS             23927..24778
                     /locus_tag="ACHA52_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8939351.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            24811..25488
                     /locus_tag="ACHA52_000599"
     CDS             24811..25488
                     /locus_tag="ACHA52_000599"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8939352.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            25976..26941
                     /locus_tag="ACHA52_000600"
     CDS             25976..26941
                     /locus_tag="ACHA52_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8939353.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            26963..27175
                     /locus_tag="ACHA52_000601"
     CDS             26963..27175
                     /locus_tag="ACHA52_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8939354.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            27250..27726
                     /locus_tag="ACHA52_000602"
     CDS             27250..27726
                     /locus_tag="ACHA52_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8939355.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            27723..29531
                     /locus_tag="ACHA52_000603"
     CDS             27723..29531
                     /locus_tag="ACHA52_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8939356.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            29608..29823
                     /locus_tag="ACHA52_000604"
     CDS             29608..29823
                     /locus_tag="ACHA52_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8939357.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            29915..30778
                     /locus_tag="ACHA52_000605"
     CDS             29915..30778
                     /locus_tag="ACHA52_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8939358.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            30815..31351
                     /locus_tag="ACHA52_000606"
     CDS             30815..31351
                     /locus_tag="ACHA52_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8939359.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            31365..31787
                     /locus_tag="ACHA52_000607"
     CDS             31365..31787
                     /locus_tag="ACHA52_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8939360.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            31717..34116
                     /locus_tag="ACHA52_000608"
     CDS             31717..34116
                     /locus_tag="ACHA52_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8939361.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            34148..36139
                     /locus_tag="ACHA52_000609"
     CDS             34148..36139
                     /locus_tag="ACHA52_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8939362.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            36162..36413
                     /locus_tag="ACHA52_000610"
     CDS             36162..36413
                     /locus_tag="ACHA52_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8939363.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            36403..37632
                     /locus_tag="ACHA52_000611"
     CDS             36403..37632
                     /locus_tag="ACHA52_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8939364.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            37629..39710
                     /locus_tag="ACHA52_000612"
     CDS             37629..39710
                     /locus_tag="ACHA52_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8939365.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            39859..43779
                     /locus_tag="ACHA52_000613"
     CDS             39859..43779
                     /locus_tag="ACHA52_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8939366.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            43780..44724
                     /locus_tag="ACHA52_000614"
     CDS             43780..44724
                     /locus_tag="ACHA52_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939367.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            44728..44931
                     /locus_tag="ACHA52_000615"
     CDS             44728..44931
                     /locus_tag="ACHA52_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8939368.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(45231..45467)
                     /locus_tag="ACHA52_000616"
     CDS             complement(45231..45467)
                     /locus_tag="ACHA52_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939369.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(45650..46021)
                     /locus_tag="ACHA52_000617"
     CDS             complement(45650..46021)
                     /locus_tag="ACHA52_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8939370.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(46018..47346)
                     /locus_tag="ACHA52_000618"
     CDS             complement(46018..47346)
                     /locus_tag="ACHA52_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8939371.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(47307..47636)
                     /gene="mobC"
                     /locus_tag="ACHA52_000619"
     CDS             complement(47307..47636)
                     /gene="mobC"
                     /locus_tag="ACHA52_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8939372.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(47894..48264)
                     /locus_tag="ACHA52_000620"
                     /pseudo
     CDS             complement(47894..48264)
                     /locus_tag="ACHA52_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            48409..48594
                     /locus_tag="ACHA52_000621"
     CDS             48409..48594
                     /locus_tag="ACHA52_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8939373.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            48752..48919
                     /locus_tag="ACHA52_000622"
     CDS             48752..48919
                     /locus_tag="ACHA52_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939374.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            49151..49324
                     /locus_tag="ACHA52_000623"
     CDS             49151..49324
                     /locus_tag="ACHA52_000623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939375.1"
                     /translation="MKSHTARCIRKKEAAHFAEAWGVPNKQNENAAGGQVVDQNIRKG
                     YSDPLLASIIGMF"
     gene            49537..50196
                     /gene="vanR-B"
                     /locus_tag="ACHA52_000624"
     CDS             49537..50196
                     /gene="vanR-B"
                     /locus_tag="ACHA52_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8939376.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            50199..51560
                     /gene="vanS-B"
                     /locus_tag="ACHA52_000625"
     CDS             50199..51560
                     /gene="vanS-B"
                     /locus_tag="ACHA52_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8939377.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT        15294 a        11113 c        12267 g        12908 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttacttc attattgcag
    15241 gtagagaagt tatttgaaga atcaattatt tttaaattta tcgtgattat cagttaaaaa
    15301 ttttgcgatt atactatttt tctcatatgg gaaattgtat tttttactaa gatctgaaat
    15361 aaatttttct gtttcaaatt ggtttttagt aattttataa aagaaatcct cgacagatga
    15421 atactttgaa atatctttat aggctaagtc catttcgtct aatagctgtt ttaaagaatc
    15481 aatcattctt tctttttcac cgtatttgaa atcaccattt cttatcttgt cttgaagttt
    15541 actgttggct cttttgaaaa atagctcttt agagatagag tgtctttggt tagcgtactc
    15601 ttcaatcaga ggattgatat tattagcaag tatctcattt agataaattt caaaatcttt
    15661 tgagtaatat tttgtaaata tattttttat tataggattc aataactcat agtctttatc
    15721 tagcgaaaac gtatcactat atctttcttg aatgtcttta aagactttgt gaggtatttc
    15781 tgggaagtcc ttaatatata gctcataccc tatatcgata aggtatgaag ttatatattc
    15841 ttctaaagtt ccccatttaa tccagtcaat tgttgaatta gtgatagttg ataataattt
    15901 cagcttttgt ggatttggta aattggtccc acgttcccag ttggatatgg tcgcacgtgg
    15961 tgaattatct attttccttc caagttcttc catagtaaaa ccacatgaaa ggcgtaattg
    16021 tttgattcgt tttcctgctt ctgaaggatt tggcttcatc ttttttctcc tttttatttt
    16081 taaagatagt atatcatatt tataaaataa aagtatttaa aagtatttac tttttatctg
    16141 gagtatgata ctttattctt ataaatacaa ataaatacat ttttttacgc aatttgtatt
    16201 tatttgtact tataaaggta gaggagttat agaataatta attaaaaagg agaggttttt
    16261 gtggaagcaa taaaattaat gatttcagaa gaacaggagg aaatgtttct aaagcagttt
    16321 tatcaaattg ctacaaaagc tattgaaaag gctcaagaaa atgcggggat gacaagggaa
    16381 tatctcaatc aaaaagaaat ggcggaatac ttaaatgtga gtgaaaactt tttaaaaaat
    16441 gctgtgagaa atgagggatt gcccgtagtt gaattagggg caagaaaatt ttattcgaaa
    16501 aaggcagtta atgagtttat gtttgctcga atgaaaagag gggaaaatat tggaagatga
    16561 tgaagtagaa attataaaaa aatgctttaa aaaaagaaaa aaagtgttcc caaaagtttg
    16621 cagactgggg gaacactcaa taataaagtg ctgagaaaac tttatttata gctattatat
    16681 caaaaaagaa ggtgaaaagg tgttttcttc tggaaatgtt gtagttgaca aacttagaga
    16741 aataaatttt gacggaaatg taatccacac acatggtacg atcaattaag aaaaaaaacg
    16801 aaaaaaggtg ttgagaagcc ctatttgaat gcaatagtta tcttagcaga aattacctat
    16861 tggtatagac ctaaaaaaat ttttaacgat gatggtcagc tacttggtta tcaaaaaaag
    16921 tttcttgaag atattttgca aaaatcatat aagcaattaa gcaaaaaaac aggattatca
    16981 gaacgagcta taaaagatgc aattgtgtac ttagagaaaa aggggattat caaaagggtt
    17041 tttagaaata tttattatga gaaaagagca gttacattat caaatgttat gtacattgtt
    17101 ttaaatgtac ataaattgat agaaattact tttcctactt tggattgcga aaaaaaggag
    17161 tatatgccga aaattcgtca gaggggtatc gaaaaaaaga gatatgtctc tttaaaaaaa
    17221 ggtagaggta tgtacaaatt tgtatatacg tatacaaaga ctacttcaca gaacatatca
    17281 aagactactc tcgttatgga agaagaggat attaatataa agaggattta cagtaagatt
    17341 agtgttcttt atgacgcaaa taacgaagat ggtcgaaaat tatttcaagt gtatcactat
    17401 ttgagaaacg ctaatgtaga tacagaagat atcagaaaaa tcattgaata tttatctgaa
    17461 aatactcagt atcttattgc tgagtgtatt ataaaacaac atgaagcatg cttttttcaa
    17521 agaaaaactg aagaagggtt atacgattat gtaacatatt ttttgaatgg acttcagaaa
    17581 agatataaaa gtcaattttt agaaaatgta gagcctatag atcagttcaa aaaagctttc
    17641 gaaatcaaag agttacctga aattactatg cataattggt tgaaaggaga agaatcatga
    17701 ttaaaaatcg cttaatagat caaatgtggt gtcttgatca aaatacagat actctcgaat
    17761 taatcgagag tatctgcttc aacacagtag ttctcgattt aagagctgaa aatgacaatt
    17821 gtgtaactca tatcataatt aatcaaaaaa tggctcagca gctctctgag agcctaagaa
    17881 aatgggctga aggagaaaac tatgaaagca attaaagaaa aaatcgttag tagaaaaata
    17941 aactggttag cactcagtgt acttgtttta atgattagcg ttgtttttac aagtaatatt
    18001 gttgaagcta gcattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat
    18061 aattctcttt atagacctta cctaaaaaaa tacaatggag atttccttag tcagttcggg
    18121 atttggcttg gatgggctgt ggtaaaaggc atgtttactg tgactgattc gattcaaaat
    18181 atgattccag atgtgttgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt
    18241 tatcaatcag tcataaatac aattgtcgtt ggattaatga tcctttcatt gatgtttgtt
    18301 ggctacaaga tgattactgg aaaaggaaca atagatttaa gatctgttgg aatgaatata
    18361 gtgatgtctg tagcgttaat tcttttaatg ccaacaatga tttctagcgg tattcaattt
    18421 gccaaaatat tttacaatga tgctactacg attacaaatt cagatgacgg agtcgcttgg
    18481 tcattgataa aacaaggagt aacagatctg gcatatatta ataaaacaga tcaatactca
    18541 agcattgata aaacggagga tcgaaataag ttaacacgaa aaaattttca acagacagat
    18601 ttaacacaag tgttgacgga taaagtgatt gataaattgg aaaaagaaaa tccagctgcg
    18661 gataatcttc gctatgaatt agttgaaaat tctaataatg aatttgttgc cacaaagttt
    18721 tctgataact tcttatcaac attttctgat agcttgaaat caggttatta tcgatatcaa
    18781 gcaaatcttt gggggatttc gattgggttg acagcgttgg caatcgccta tgtgttcagc
    18841 gcgtttgtga tcattacagc aatattagaa cttgcattta aacgtgtgtt aggtgttctt
    18901 gtttttgcga cagatattga aacaggacaa cgttcaaaag ttgttttatc cgatatttta
    18961 caatgttact taacagttgg atttcaaggg tttggactat ctatgtttgc tatgtttatc
    19021 aattttttaa atgctggaca aggtatctcc acaaatattt ttataaaaac aattgcttat
    19081 atctgtgctg tgtttgtgct gataaaaggc agtggaacag tcatgagata ctttggtgtt
    19141 gatattggac tcaaagaagg ctatgggcaa cttgcttctg cttttggtat gggcgcaatg
    19201 ctgtttagaa aaggatcaac tggatttaat cgtgctaaag gaagcggaaa tggaaatgga
    19261 tcagacagtg gagaaggaga agatcgaaaa ccagaaaaga atttcggcga aactctatca
    19321 aaaaaagctg gcaaaactgg aagagctttg ggatatgccc atgaacgtgg attatcaggt
    19381 ttagcttcag atggagctac aatggctagt gaaagagcaa taaaaccatt taagagtatg
    19441 cgtgatatgg ctaatgatac caagaataag ttcaaagaag gtttagatga tggtacagtg
    19501 tccgctatta ataaaaatag caaacctatg ttagctaaaa ataaagaaga tgaaactgga
    19561 aaatatgctg attccatgcc aacacgtctt tcggatagaa aagatggtgc taaggttgaa
    19621 aatgccgata gaattatgtc tagttctgaa cgtatgcgtg aagcaatgaa aaataatgct
    19681 gaatcgaata ataatcctgt aagtgcaatt cagcaaaaag ttcaacaaga tattgaagaa
    19741 cgtaaaaatg ctatgcacgg tcaagctaag tctgctgaag aattaattaa tcagaagcga
    19801 caagaagcta aatacacgcc tgaagcaatg aatcgtgagg aaatgttaag gaaacgtgta
    19861 gaagggcgta caggagctaa tacggacgaa aaagaagcgt tgaccaaaga aagaatccaa
    19921 gaagctaaaa actcaaatac cggattagaa aaattggtca aagaaaatct acaaaattct
    19981 acaagtagtc aaggtggccg atcagtagat gtccgagaaa atattcaagg ttcattgaat
    20041 ggtcatgtcg gacggaatgt tgatgtaaga gagaatttac aaagctcttc aaatggtcaa
    20101 ggtactaaga caattgatgt tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt
    20161 aaatcaatgg atgtaagaga gaatgtacaa aattctacaa gtagtcaagg gtttaaatca
    20221 atggatgtaa gagagaatgt acaaagttct acaagtagtc aaggattcaa aacagtagat
    20281 gttcgggaga acgttcaaaa agatagtgga gaaatgaaag aaaaaacaca aaaaatcaat
    20341 atcgttgaag acagaaagtc agcgaaaaaa tttgattcaa atcatgaaac agtgattatt
    20401 gatagtgaaa tccgtgaaaa tgataaggga tcaaaaccaa gacgaagatt tacctatgaa
    20461 gacaacgagt tattcagaga tacacttaat gatcctaatc cgcttttcga taatctatta
    20521 aaaaaataaa agggggaatt gtcatgaaat taattctaaa gattagtacg catgatttct
    20581 tagaacttga cgaattatgt gaatcagtaa agaacgcaac aaatgaaaaa atcacggtca
    20641 ctaattatat tgaactgatg attcaagaag aattaaagat gaaacatgag ataaaaaaca
    20701 acttgaaata ttgttatcta gaaaaaagtg aagagataat ccatgaatta aatggctttt
    20761 aaaaattgta tttgacatga atttacgaca aggaattagt ggggattaag cgattattct
    20821 cttccacttt agagaaataa tgggttaaaa agccagaata cgtagagcaa tattgacaac
    20881 ctataaagga gctattgttg aaaattgaag taacaaaaag ataaagggag gtttacatct
    20941 atcagattaa aagtgatagt acacccatga aacctgcaaa agagttgaac ctaatgtata
    21001 tcggtttaac tctttttatt tgcaaattaa aaaaatatca ccgaagtgac gaatgaaact
    21061 atgaccaaat taagcaagaa aatatatctt gcaaataaag tagtataaaa agatattgat
    21121 aaagccaatc aaaactttga atttcaaacg ttagataatc aaacagatac aaaaaatgaa
    21181 atcagttcta cgcgtacgat tattaaaaat aataaatttt cactagaaga aaatggaaat
    21241 aaaatcaaat aattaggaag aaaaaacaaa gcaattagaa aagcctgcaa gcaaatttga
    21301 gtaatcgaga tccaattttt ttaaggaagt ttaacaaaaa tatgtaattc tattatcact
    21361 attaaagaga aagttggagc ggaatataaa gaaaagagaa aagtaattaa acgcaaaaaa
    21421 atgtagaact taaaaaatat aaagaggaac aaccgttaaa attaggaatg atgttgtgta
    21481 aacattataa ttgctatgat ataaatgaag tgttaaagaa agcaaattta aaccaatttt
    21541 gtgaacagaa aagccttagt cactatgacc aaaactacgg acgaaactat gaccaaaagt
    21601 aggtactaaa ttatgaccga aaataggtcc aaaatggatt tttttatgat atcaaaaatt
    21661 atgaaatgat tttgaaagaa aaccagttgg cacagccaac ttagccacct tcctgtagga
    21721 aggtgctatc agttttccct tatgctcttt tctttctttt ttgtttcata tgtcaaatgc
    21781 cattttgaaa tgtaccatct taagaaattt atccttaaaa acaattgcgt tattggataa
    21841 aaagatgcta cattatctta aaaaaggaat caaatatgat gaatttagtg gctagcatat
    21901 cgtgtgatta agatacttta tagtgtaagt tgaaggagaa aaaatattta aaatatactt
    21961 cccatttcag tgggaagtaa aattaatctt gttgtttcta ctagtaatgt atctttttgg
    22021 aaactatgta acgatgtaaa caagattttt ttcagaagaa aaaacgataa aagttgtgat
    22081 aaacgattta aaaaatagga gaaaatatat caagcgcatg gtatgaaacg cacatttaaa
    22141 aagattcgac taactaaaaa aataagttta caaagcattg ttgtgtatta caagtgatct
    22201 atacactgaa aaatgataga gaaaatctga tacgtccgca acaatggaca attgaaaatg
    22261 aaagtgagga aattggaaga atggctgatg aaaataaagt tgttccgctt gaaacactag
    22321 tgataaataa ggaagagttt gagcaacttt ttcttgattt ttttgttcaa tatgacagga
    22381 tttatgattt tagttcgtca ctagatttat ttgaattaga ggcagaaata atagaaaaca
    22441 gtcgagagga gaatggtaaa atcatcattt cagtagaaaa attcaatgaa ttattctcag
    22501 aaacatttga aatagataca gattattatc aaccacaaga agttttccaa tcatttagaa
    22561 atcagattat tcgctcaggt aagaccgtaa actgaaaaac aataaaagaa acgttgaaag
    22621 aataattatt ccttcaacgt ttctttttta gaatgtttta atgaccaaag cagtttcatt
    22681 gtcaggctta ggtaattcat gaaatcccgt atttatagtg ctcaattgat caactaacca
    22741 tgttttactt tttgaattgt agataaatct aaaattaata tcgttattat cgtgttgaag
    22801 gggcgtctcc aaataatctg ctgtatattc atagtcaacg taacctgcta atacaaaaga
    22861 gagtatccct ttatttaatg ttagatttaa tggataatca tacgacttta tttttttcaa
    22921 cttcatattg tacatatctt cagccataaa agttgagttc gtgatttcat cttgggtctc
    22981 attaaaaaag ttatccgaat aatcgcttat ttttgaaaag tctttattgg caccagcctc
    23041 aaaaaattgt tcaacttttt cagtataaag ttgtgctaac gctccttttt caatattaac
    23101 tttaatattt tcatgtctat caatttgtgt gctgttcagt aagggaagat cagcttttag
    23161 ttttatatcg tcttgatttc tttcaatttc aattggcaaa atcttttcag tagattcagt
    23221 ttctgataaa ggaacatcta gttttttatc atgataataa gcttcaaatc catctggaac
    23281 cggaaaaaaa tgtattgggt aattcttttc tgaggttgtt gtactagttt ccgtacttgt
    23341 agaacttgtg tcagaagtgg tacacccgct tatagttccg atgagtaaag cgcaagttag
    23401 aatgattgca aatttcttca aaaaaattcc tccttaaaat tgaatatgga ctaatactag
    23461 tcccattgta gcataccgaa tctttaagat taataagaaa aaggactaat tttagtccaa
    23521 aaggtgtgct attcttgaaa gaggaaaaaa ttattgataa agaaaatgta atcggtagca
    23581 atattagacg agtccgatta gaaaaaggaa ttggtcaaac cgagctgatt agagacctac
    23641 agcttcgtaa aattgctata actagggaaa cgttggttaa gattgaaggt ggccgccaac
    23701 acattaagtt ggatcaattg aaagccatca aagatattct acaggtgtct tatgaagatt
    23761 tgcttcaatg actacttgta gtcatttcgt ttgaaattat tatgttacac ccttatgtgt
    23821 cacgaagaaa tactggtata ataatgaatg ggatatgtaa cgatagggta tactgttatg
    23881 ttgcctaata aattgtggat caaaatacaa gaatggaggg atatccttgt ataaggcata
    23941 tgaattggat tttacaaatg ctgacttaaa tgcattctcc tcaagctcgc aatcatatac
    24001 acatgtagta aatcaaatag atcacaacca aaaatctatt aacaagagaa tagaaaatct
    24061 atttgaaagt gaaaatgatt tagttgatta ttattctaag gacagcaaaa ttctagatgc
    24121 agacgaaatt attaaagatt ggtttccaac tataaaagca gatattttta tatctcactc
    24181 tcatgctgat gaaaaattag ctatacgttt tgcatcatgg ctatttgaaa attttggtct
    24241 tactgcattt attgattctt cggtttgggg atacagttct gatttattaa aaaaaataga
    24301 ccaaaaatat tgctataagg aacaaacaaa aacatatgat tatgataaaa gaaatgtaac
    24361 aactagtcat gttcatatga tgctttctac agctttaaat aatatgattg atagtacaga
    24421 atgtctgttt tttcttaata cacctaattc aattagtttg agcaatgaaa tcactaatga
    24481 acagaaattt acgtattcgc catggctata tagcgaactg acgacagctt caatagtgga
    24541 aaaaaagaat ccacgattgg aaagtaatcc tcaaatgagc accgaggatg ttagaagcat
    24601 tataaagcac tattcttcgc aagaaaatct gcaaataaaa tattctttaa atttaagtga
    24661 attaacttat ttacgaaaag ctgatttgaa aaaatgggct ttagatgctg tggaatgcac
    24721 taaaggaagt aatacattag atattttata taaaaacacc attttcacta atctctaatc
    24781 aatttactga atcgaaagga accgggtaga atggataaga aagttttaat aaagaggttc
    24841 tctgaagcta ttgaaaaagg aaatgcagca atatttgcag gtgcaggatt atcgatgtca
    24901 caaggatatg taagttggcc ggaactactt aatgatcctg caacagaaat cgggttggat
    24961 tctaaaaaag aaacagactt agttactctg gctcaatatt ataaaaatga aaatggtgga
    25021 tcaagaggaa ttttgaatca gatacttatg gataattttg gtgaagaatt agaaatatct
    25081 gaaaatcatc gaatactagc aagtttacct attgaaactt attggacaac taattatgat
    25141 catctaatag aaaaatcaat ccgagaagct tataaaaacc ctcaggttaa gaaaaattat
    25201 actcagttag caacaactaa tccaaatgtg gatacaattg tttataaaat gcatggtgat
    25261 atagatgatg tcagttcgac agtaataaca agagatgatt atgaaaaata tgatgattcg
    25321 tacgcgttat ttaaagaaac tttaaaagga gatttgttaa caaaaacttt tctcttttta
    25381 ggatttagtt ttacagaccc taatttagaa agaattttat cagatataag atgggttttg
    25441 agagaaaacc aaagacctca ttattgtata atgagaaaaa tattatagaa aaaattttag
    25501 gttatcgctg aaagccccgg aaatacgggc atttcgcagg atatgagaac tcaatttact
    25561 accaatttac tactttcgga ttgagccgca tatatgctga accatactgc taatacccgc
    25621 caccccaaat acggcacatc ataaattgaa tacgacaccg taaacgcggc gttcctcttt
    25681 cccgtcatgg gagaacagga cgccgctttt ttatgcccaa atagagaaag gaggcgacgc
    25741 catgctattt acttccggcc ctgaccgggc gtttgaacgg ctgatgcagg aaaagcccgg
    25801 tttcgatcac tacgacggcg gcggggcgga cgcgccggag gattgcggca cctgccgatt
    25861 ttaccgcccg gattggaaat atcaattctg ccagtttgca gagtgtccct atcagccggg
    25921 caagctgacc gactatgcag cggtcaaatt cacagtgaaa ggagcgaaca acgctatggc
    25981 agttttccgt gtggaaaaga acaaaggcta tacggtgatg tcaaatcacc acctgcggaa
    26041 taagaacctg accttaaagg ccaagggcct gctgtcgcag atgttgtcct tgccggagga
    26101 ttgggactat accctaaaag gactgtccta tatcaaccgg gaaagcatcg acgcgatcag
    26161 gacagcggta tgggagcttg aaaaagctgg atatatccgg cgtgagcagg gccgtgacgc
    26221 aaaaggcaaa atggccgata tggtctatac catctacgaa cagccggtat tggaaaaccc
    26281 ggtattggaa aatccaacat cggataaccc ggtgttggaa aatccgacat cggataatcc
    26341 ggcgtcggaa aatccaacgc aattaaataa agaagtacaa agaactgact tatcaaaaaa
    26401 agaaaaaata attacagatg gacaaagtac ccattccatt cctatcccat ccccgacccc
    26461 tgcccctatg gagggcgagg cagcggctac gccgccggaa cggaaaagga caggaaagga
    26521 tgaggctgta cggatttatc gggaaatcat tttggagaac atcgagtatg cctatctcat
    26581 tcaggacagg agcattgacc gtgaacagct tgacgaaatc gtagacctga tgctggaaac
    26641 cgtctgcacc gcccgaaaga caatccgtgt cgccggggac gactaccctg ccgagctggt
    26701 gaaatccaag tacatgaagc tgaccggcga acatatccgc tttgtccttg actgtctgcg
    26761 ggagaacaca acgaaagtcc gcaatatcaa acaatacctg cgggctatgc tgttcaacgc
    26821 ccccagcacc atttccaact actacacggc ccttgtcgcg cacgacatgg cacagcccga
    26881 ttggggaaag cctaaatccg gcctgccgga ctattcctgc ggcgagggcg aaagcctgta
    26941 acgagaaaag gaggacgacc ctatgaagca gggcgcattg atttttgacg agcgaacaga
    27001 ccgttacgac atccgctttg atctggccga ctattacggc ggcctgcatt gcggggagtg
    27061 tttcgacgtg atgatcggcg gcaaatggag gcccacccgc attgaaatgg ccgaaaactg
    27121 gtatcttgtc ggcatccggg ccgacgacct ttccggcctg cgggtgcgga tttgacctat
    27181 atctacctgc ggcgtctggt ggttccgcct tagacgccgc ttttgacttt ctgacaagga
    27241 gggatgcgat tgcaggaaga agttactcaa aagaccattg ccctgtccat gaaaacgggc
    27301 aagctcacgg ctcaagtgct gcaagcggcg ctgaaaaagt acctgcaaca ccgggccaag
    27361 ggcaaaaaca cgctgcacca cggccagcag agcctaaagc agcttaaaaa gcatggggct
    27421 gatctctcca acattgagat caccgaggcc aacattggag cgttcaagcc atgcgccaag
    27481 aaatacggcg tggattttac cttacgaaaa gataagacca cccagccgcc ccactacgtt
    27541 gtgattttca aggccaagga tgcagataac cttgatctgg cgtttcggga gtttaccgcc
    27601 aagacgcttt ccaaggagca gcggccctct atccgcaagg ttttgtccgc tgtgaagcaa
    27661 aaggcggcgg cccagaccaa gcagcgggcc aaggagaaaa tcaaggaaag ggggctggaa
    27721 ctgtgaagcc tgacgtaaag aaattgctga tcctgaacct cccgtatctg atttttgtgt
    27781 acctgtttgc gaaatgcggg gaggcgtacc ggctggccgc tggtgctgac ctgtcgggga
    27841 agctcctgca ttttgcggat ggttttgcgg cggcttttgc caaccctctg ccaagcctgc
    27901 acctgtttga cctgtgtatc ggtatcgccg gtgccgtgat cgtgcggctg gtggtgtact
    27961 gcaagagcaa gaacgtgaag aaataccgca aaggcatgga gtatggcagc gcccgctggg
    28021 ggacggccaa agacatagcg ccttacatcg accccaagtt tgaaaataat atcctgctca
    28081 cccaaacgga acggcttacc atgaccggca ggcccaaaga ccccaagaca gcgcggaata
    28141 aaaacgtgct ggtgatcggt ggcagcggtt cagggaaaac gagatttttc gtgaaaccca
    28201 acctgatgca atgctttccg accacggact atccgacttc gtttgtggtg acagatccga
    28261 aaggcacttt ggttctggaa accggcagga tgttccagca ggcgggctac cgcattaaaa
    28321 tcctgaatac cattaacttt tccaagtcca tgaagtacaa cccctttgtc tacatccatt
    28381 cggaaaagga cgtgctaaag ctggtaaata ccctgatttc caacaccaag ggcgaggggg
    28441 aaaagtcagc ggaggatttt tgggtgaagt cggaacggtt gttttattcg gccctgatcg
    28501 gctatatctg gtacgaggcc ccagcggagg aaatgaactt ctcaacgctg ctggaaatga
    28561 taaacgccag tgaagcccgc gaggacgacc cggagtttca aagccccgtc gatctcatgt
    28621 ttgagcgttt ggagcagaaa gaccccgacc attttgcggt gcgccagtac aagaaattcc
    28681 tgctgtccgc aggcaagacc cgttcttcca tcctgatttc atgcggtgcc agacttgcgc
    28741 cctttgacat ccgggaggta cgcgagctga tggaggacga cgaaatggag ctggacacca
    28801 tcggggacga aaagacggtg ctgtatctca ttatgagcga tacggacacg acctttaatt
    28861 tcattctggc tatgctccaa agccagctaa tcaacctgct ttgtgaccgg gctgatgatg
    28921 tgtacggcgg gcggctgcct gtccatgtac gcctgatttt ggatgaattt gccaacattg
    28981 gccaaatacc caatttcgat aaactcattg ccaccattcg aagccgtgaa atatcggcct
    29041 ctattatttt gcagagccag tcgcagctaa aagcgattta caaggacgcc gccgagatca
    29101 tttcggataa ctgcgatagt gtgcttttcc ttagtgggcg cggcaaaaat gccaaggaga
    29161 tttccgatgc gctggggaaa gaaacgattg acagtttcaa caccagcgag aaccggggaa
    29221 gccaaacgtc ccacggattg aactatcaaa aattaggaaa ggagttgatg tcccaagacg
    29281 aaattgccgt gatggacggc ggcaagtgta tcttgcagct acggggtgtg cgcccgtttt
    29341 tatcggagaa attcgatatt accaagcatc cccggtacaa ataccttgcc gacgccgaca
    29401 agaagaatac ctttgacgtg gacagatttt tgactatcac gcgccggaaa cggcagcaag
    29461 tggtaacgca ggatgaagta ttcgacctgt atgaaatcga cctgtcggac gaggacgccg
    29521 ccgctgaata aacagcggct tttttcattc caacaacaaa atcaaaaaat cattatgagc
    29581 cggaaaccgg cagaaagagg taaatttatg gcatttttta attccgcagt tactactttg
    29641 cagactatcg tgatcggact gggcggcgct ctgtgcgtat ggggcggcat caatctgttg
    29701 gagggatacg ggcaggacaa tccgggggca aaatcgcagg gtgtcaagca gcttgtcgct
    29761 ggcggcggcg tggccctgat cggtgtcact ctggttcccc tgctgtccgg gctgctgggc
    29821 tgatcctggc ctttgtccct gtccaaaccc ttaactaatc gcctccgcgt cctcccggcc
    29881 agaccgggag ggcgcgggaa aggaggtgct acccgtgatt agtgaaatca ttgaagaatg
    29941 gataaaaggc atcctcatgg acggtatcac gggcaacctg tccgggctgt tcgatacggt
    30001 aaatgccaaa gtcggagaaa tcgccgccga cgtgggacga accccgcagg catggaacag
    30061 cggtattttc aatatgctgc acagcctgtc ggaaaccgtg atcgtcccca tagccggggc
    30121 catactcgcc cttgtcatgt gctatgagct tatccagatg ataacggaaa agaacaatat
    30181 gcacgacttt gacagctcca tgttcttccg ctggatattc aaatccgcgt ttgcaatcct
    30241 gatcgtaacg aacacatgga acatcgtcat gggcgtattc gacgccaccc aaagcgtcgt
    30301 caatcaaagc gccggtgtga ttatcgggca gacatccatt gattttaaca ccctgatacc
    30361 agaccttgaa agccaattgg aggctatgag cgtgggcgct cttttggggc tgtggttcca
    30421 aacccttgtt gtgggcctca ccatgaatat cctttccatc tgcattttcc ttgtgaccta
    30481 tggccgcatg gtggaaatct atgttgtcac gtcgctgggg cctatccccc ttgccacaat
    30541 gggcaacgcg gaatggcgcg gcatgggcca agggtatctg aaatccctgc tggcgctggg
    30601 cttccaagcc tttttaatca tggttgtggt gggtatctac gcggtgctga tacagaatat
    30661 cggttccgct tccgacatat ccggcgcaat ctggggctgt atgggctata cggtgctgct
    30721 gtgcttctgc ctgttcaaga ccggcagtat atcaaaggcc gtattcgggg cgcattgatc
    30781 ccaaaatcca agcatacaca ggaggaactt gtttatttca acgtacaaca tcatttacgc
    30841 tgacccgccg tggcggtatg actgcaaaaa cgtacagggc gcagcggaaa atcactattc
    30901 caccatgagc attgacgagc tgtgcgccct gccggtggaa cggctggcgt ccaaagactg
    30961 cctgctgttt ttgtgggcca cattccccat gctgccggaa gctctgcggt taatcaaagc
    31021 gtggggcttt tcattcaaga ccgtggcttt tgtatggctg aaacagaacc gtaaaagcct
    31081 tacatggttt tacgggctgg gccgctggac aaggggaaac gcggaaatct gcctgctggc
    31141 aacacacggc aagccgaagc gtcgttctgc ctctgtccat cagtttatta tcagcccgat
    31201 tgaacaacac agcaaaaagc cggatgtgac ccgtgaaaaa atcgtagagc tggcgggcga
    31261 tttgccccgc gtggagctgt ttgccagaca gaaaaccccc ggctgggacg tgtgggggaa
    31321 cgaggtggaa tgtgacctta tcctgacgtg aaaggaggtg ccaaatggct tatgtaaccg
    31381 tccccaagga cttaacccat gtgaaaagca aggttgtgtt tgggcttacc aaacggcaac
    31441 tgatctgttt ttccggggcg ctggtgatcg gcgtcccctt atattttctc acccgtgact
    31501 atcttaccaa cagcgcggcg gccctgctca tggtatttgc catgctgccg ggctttttgc
    31561 ttgccatgta cgagcgtcac ggccagcccc ttgaagtggt gattatgcag atcatccagt
    31621 gctgcttcat ccagccaaag gagcgtccct atcagaccga caacacctac accgcccttg
    31681 tgcggcaata ccagatggaa caggaggtaa aggccattgt ccaaaaagga aatcccaaga
    31741 gaaaccgaaa aaacaaagct gacccgcgcc cagcgaaaag aaattgacgc ggtaatccgt
    31801 aaatataagg gcgacggcag gccccacacg gcgcagcaga gcatccccta cgaggtcatg
    31861 tacccggacg gcgtgtgccg ggtatcgccc ggcgtctttt ccaagtgcat tgagtttgcg
    31921 gacatcagct atcagcttgc ccagccggac acgcagacgg ctatctttga aaaactgtgc
    31981 gacctgtata actatgtgga cgcctccatt catatccagt tttctttcct caatcggaaa
    32041 gttgacccgg tgcagtacgc caagagcttt gagatcgcgc cgcaggggga cgacttcgac
    32101 gacatccggg ccgagtacac cggcattctg caaaagcagc ttgcaaacgg caacaacggc
    32161 atggtaaaga ccaaatacct gacattcacc atcgaggccg aaagcgtgaa agcggcgcga
    32221 gccagattga agcgcatcgg ctttgacctg ttaggctatt tcaagagcat gggggccgta
    32281 gcccatgtga tggacggctg ggaacggctg aacctgctgc atggcgtgta ccacccggac
    32341 ggcgagatat tcaattttga ctggaagtgg ctggccccgt ccggcctctc caccaaggat
    32401 tttattgccc cgtcctccct gtgcttcggc aatgcaaaga cattcggcat gggcggcaaa
    32461 tacggcgcgg taagtttcct gcaaatcctg tccccggagc tgtcggacga tatgctggcc
    32521 gacttcctca atacggaaag cggtgtttta gtcaatctcc atgtgcaggc cattgaacag
    32581 accaaggcga tcaagaccat caagcgcaaa attaccgacc ttgacgctat gaagattgcg
    32641 gagcagaaaa aggccgtccg cagcggttac gatatggaca ttcttcccag cgaccttgcc
    32701 acctacggcg aggacgccaa gaagctgctg acaaagttgc agacccgcaa tgaacggctg
    32761 ttccagttga cgttccttgt cctcaacgtg gccgacacca agcaaaagct gaataacgac
    32821 gtgttccaag ccgcaggcgt ggcccaaaag cataactgtc cccttgtccg gctggactac
    32881 cagcaggaac agggccttgc aagcagcctg ccgctgggcg tcaatcagat caagatacag
    32941 cgcagcctta cgacttccag cgtggccgtg ttcgtcccct ttgtgacgca ggagcttttt
    33001 cagggcggcg cggccatgta ttacggcatc aacgccaagt cccgcaacat gattatgctg
    33061 gacaggaagc aggcccgttg ccccaatgcc ttgaagctcg gcacccccgg cagcggcaaa
    33121 tccatgagct gtaaatccga gatcgtcagt gtgttcttaa cgacgcctga cgatattttt
    33181 atttcagacc cggaggccga atattacccc cttgtcaaac ggctccacgg acaggtaatc
    33241 cggctgtcgc ctaccagcaa ggactttgtg aacccgctgg acatcaacct gaattattcg
    33301 gaggacgata acccgctggc cctgaaatcc gactttgtgc tgtcgttctg tgagcttgtc
    33361 atgggcggca agaacgggct ggaagccatt gaaaagaccg tgatagaccg ggctgtgcgc
    33421 gtgatctacc ggccctatct ggcagacccc cgcccggaga atatgccgat cctgtccgac
    33481 ctccacaaag cgttgttaga tcagcatgtc ccggaggctg accgggtggc gcaggccctt
    33541 gacctgtatg tgtccggctc cctgaacgta tttaaccacc gtaccaacgt ggacatcggc
    33601 aaccggcttg tgtcctttga cattaaggag ctgggcaagc agctcaaaaa gctggggatg
    33661 ctcattgtcc aagaccagat atgggggcgc gtcacggcca accgaagcca aggaaaggcc
    33721 acatggtatt ttgccgacga gttccatttg cttttgaaag aggaacagac ggcggcctat
    33781 tctgcggaga tttggaagcg gtttaggaaa tggggcggca ttccaacagg tgccacccag
    33841 aacgtcaaag acctgctttc ctccccggaa attgaaaata tccttgaaaa cagcgacttc
    33901 attacgctgc tcaatcaggc gtccggggat agaaaaatcc ttgcggaacg cttgaatttg
    33961 tccaccgaac agcagaagta catcgacaat tccgaacccg gcgagggcct tttgatcttt
    34021 gaaaacgtgg tgctgccctt taccaacccc atcccgcaca acacccagct ttacaagatt
    34081 atgacgaccc gcttaaacga ggtggcggga gtatgacccg gcgacgccac aaaggaggtg
    34141 aaccaccatg aaagagctga aagccaaggc gaaagtgacg caggccatga cccgcgacgg
    34201 gctgacgctg gaaaatcagg ccaccggcga ggtggagaac atttccagcc gggaggccga
    34261 acaggatttg tccgcgccgg ggccgggcgg caccgccgat aagctgctgg aacgggccga
    34321 cgccatccat gacggccata aggccaagaa agccgcgaag caggccgggg ctgtggccgc
    34381 cgagggggac gctgccctgc accggccatc cgcccggctc caactgtcgg aggaagaacg
    34441 ggccgacccg gatttacagc cctatatccg aaaatcggac aaacgggccg ataagctgga
    34501 taaggcccgc gccgccatcc ccaagaagcg cgtcccggtc aaggaaaccg tctttgacga
    34561 ggcaagcggc aaagcaaaga ccgtcctgca ctttgacaaa caggacaagg ggcctccctc
    34621 cctgaaaccc aatccggcag gccgtcctgt ttccgaggtt tttctattcg gccacgggaa
    34681 aatccatgaa gtcgagcatg aaaacgtggg tgtggagggc ggccataagg gggaggaact
    34741 tgccgagcgg caggccggaa aagccatccg cagcaccatc cggcaccaca ggctgaaacc
    34801 ctactgggac gcggaaaagg ccgagcgcag gctattgtct gccaacgcgg agtatttcta
    34861 ccagaaatct ttgcgggata acccggagat cgcacaggcg gccagcaacc ccatttcccg
    34921 gttctggcag aaacagcaga tcaagcgcca gtacgccaag gccgcccgga cagcgggcca
    34981 gaccgcaggc gcggcggcac agggcgcggc caagacagcc ggggcctcca cgaaagcggc
    35041 caaggccgca gccaaagaaa gtgaacgggc ggcctccttt gtggcccgcc actggaaagg
    35101 ggcgctggcc gtccttgtgt tggggctttt gatgctgctt atcatcggcg gcttacagtc
    35161 ctgcaccgcc atgttcggca gcgccggaag cggcatcacg gcgtcctctt acttttccga
    35221 ggatagcgat atgttggccg ccgaggacgc ctacgcggag ctggaagccg agctgcaaga
    35281 ctatctggac aactacgagg ccacccatga ctatgacgaa taccattttg accttgacga
    35341 gatcagccat gacccctatg tgctgatttc cattctgtcg gcgctccatg acgggatatt
    35401 taccatcggg gaggtacagg gtgatttgtc catgctcttt gacaagcaat atatcctgac
    35461 agaaaacgtc gtggtagaaa cccgttaccg caccgaaacc gatacatgga cggacgcgga
    35521 gggcaacacc catacagaca cctaccaagt gccgtatgac tattacatct gctacgtcac
    35581 gctggaaaac ttcgacctgt cccatgtgcc agtctatatc atggacgagg aaacgctaag
    35641 tttctattcc ctgtatatgt ccacgctagg aaaccggcct gatctgtttt ccggcagcca
    35701 gtaccccaac gcctccaccc tgacgccgcc gacctactac gacgtacccc cggaggcttt
    35761 ggaggatgcc acctttgccg ccatgctcaa agaggcggaa aaatatatcg gttatcccta
    35821 tgtgtggggc ggcagctctc ccagcaccag ctttgattgc agcggcttca tttcgtgggt
    35881 aatcaatcat agtggatgga acgtgggacg gctgggggcg cagggcctat ataacatctg
    35941 cacccctgtt tccagttcgc aggccaagcc cggcgacctt gtatttttca agggaaccta
    36001 cgacaccccc ggcgtgtccc acgtcggcct gtatgtgggc aacaatgtga tgctgcattg
    36061 cgggaacccg atttcctaca cgaaccttac ttcaaactac tggcaatccc atttttacag
    36121 ttacgggcgt ttaccttaac gccagaaagg agctttcaat tatggcaacc aaacttgacc
    36181 gcatcgaaaa ggacatccag aaaaccaagt ctaagatcgc ggagtttcag aaacagcttc
    36241 gggagctgga aacgcagaaa accgaacagg agaatttgca gatcatccag cttgtgcgcg
    36301 gcatgaacat gaagccggag gaatttgccg cgttcttacg cagcggcgca atgcaggccg
    36361 cccctgccgc aaccccttac catgaacagg aggacaatgc ccatgaagaa taagaccaga
    36421 cggatttccc gtattctccc cgcacttctg gccgtgatgc tctgcgtcac ggcctttccc
    36481 gttaccgccc ttgcaggcgg caatgacccg gcaccggccc cgctgccgga ggctacaacc
    36541 gaacccgcta cgggcggttt ggagcctgaa acagacgaaa ccggcaccgc cacggacggg
    36601 acagaaaccg aggccgaaaa agataccggc cctctgaccg gcaaggacat caccgacctg
    36661 ttttccgcgc tgttcggttc caaggtgagc attgccgcca cggacgacgg catccagatc
    36721 accaccggca ccgacaaaaa agaacctgaa cagaccggca ccgtcaccac caacggcggc
    36781 aggctgaatg tccgcaccgg cgcggggctt gacaagaccg cgttcaccca gcttcccaat
    36841 gggacgacgg ttgaggtagt cggcacagac ggcgactgga ttaaaattct cttgccggag
    36901 cgcatcggct atgtccattc cgactatatg accgtcagcg aaaaggaagt agcggctacc
    36961 ggcgagggcg gtttttccct ctccattgac ccggaggaaa ttgcttctct gctggaactg
    37021 ttcaatggcg gcggggccag cgccgccctg tccccggacg ggaacctgtc actgattgac
    37081 gacatcggca gcgccgcgaa aaatggcaag cagtttatca ccgtggaaac caaaaacggc
    37141 aatgtgtttt atctgattat tgaccgggac gacgagggaa aagaaactgt ccatttctta
    37201 aatcaggtgg atgaagccga tctgctgacg ctgatggagg acggggaaac cgtcaccccc
    37261 gctgctgtct gctcctgtac cacgaaatgc aaggccgggg ctgtgaacac gaattgcccg
    37321 gtatgtaaaa ccaacctgac cgagtgcagc ggcccggagc cgcaggaacc ccagccggag
    37381 gaacccgaag ccccggagga agaacccaag ggcggcgcgg gcggcctgat cgtgttcctg
    37441 ctggtggcgc tggcgggcgg cggtgcggcc ctttactatt tcaagttcaa aaagcccaag
    37501 gctgaaacca ccggcagcga cgatttgggc gagtatgatt ttggggagga cgaggatttg
    37561 gaggacgagg aacccgaaac cgagtttgac ccggaagctg attttgtgag tgaacaggag
    37621 gacgaggaat gacatataaa cttgtaatcg cggaaaaacc cagcgtggcc cgcagcattg
    37681 cgggcgtgat cggggccacg gagaaacacg acggctattt ggaggggaac ggctatctgg
    37741 tgagctggtg catcggacat ctggtgtcct ttgccgacgc cggacgctat gacgagcgtt
    37801 tcaaaaaatg gcgctatgag gatttgccca ttctcccgga aacatggcag tacatcgtcc
    37861 ccgacgacaa gaaacagcag tttgacgttc tctgttcgct gatgcagcgc cccgacgtga
    37921 ccggccttgt gtgcgccacc gacgccgggc gcgaggggga attgattttc cgtttcgtct
    37981 accagatggc gggatgccaa aagccctttg agcgcctttg gatttcctct atggaggaca
    38041 gtgctattaa ggacggcttt gcccacctga aaccgggcgg cgactatgat ccgctgtatc
    38101 aatcggccct gtgccgggcc aaggccgact ggctgatcgg cattaacgcc acccggcttt
    38161 tttccgtcct gtaccacaag aacctgactg tgggccgcgt ccagacgccg accttaaaaa
    38221 tgctggtgga cagggacgcc aagattaccg atttcaagaa agagaaatat cacatcgtcc
    38281 atatcacggc gggcggcgcg gacgcggtaa gcagccgctt ttccgacgct gccgaggcaa
    38341 acaccgtcaa ggcggcttgt gcaaaagcgc aggccgtttg cgtttccgtg acgcgggaga
    38401 aaaagacgga acagccgccc aagctctatg accttaccac cttgcagcgg gaggctaacc
    38461 ggcttttcgg ctataccgcc aagcagaccc ttgactatgc ccaggcactg tatctaaaga
    38521 agctgctgac ctatccgaga acggatagtc aatatcttac cgacgatatg ctgcccgccg
    38581 tggaaagcct tgtgtccggc ctgtgggagc tggtgccgtt tgccaagggc ctgaaccttt
    38641 ccccgcagtt tgaccgcatc ctgaacagca aaaaggtgtc cgaccaccac gccattatcc
    38701 ccaccgctga atttgtgaag cagggctttg gcactctggc cgagagtgaa aaaaagctgc
    38761 tgtccctgat ctgctgcaag ctcttatgtg ccgtggccgg gccgcatatc tatgaggccg
    38821 tcaccgccac atttacctgt gccgggcagg agtttaccgc caagggcaag accgtccttt
    38881 cccccggctg gaaagagatt gaacgccggt tccgttcttc cttaaagacc gacgccgacg
    38941 aggacgccga ggcggtgcgg gagctgccgg agctttgcga gggccagacc tttgcagacg
    39001 tggccgcctc tgtcaccgag catttcacca cgccgcccaa gccctacacc gaagatacgc
    39061 ttttgtccgc tatggaacgg gccggggccg aggatatgcc ggaggatgcc gagcgcaagg
    39121 gattaggcac accggccacc cgcgccgcca ttctggaaaa gctggtgcag atgggctttg
    39181 tccagagaaa aggcaagcag ctcattccca ccaaggacgg catcaatctg gctgtggtgc
    39241 tgccggaggc gcttacttct ccccagctta ctgccgaatg ggaaaaccgg ctgaccgaga
    39301 tcgccaaggg gcaggccgat cctgacgaat ttatggacgg catcgagacg caggcgcggg
    39361 agctggtgca gacctattcc tgtatcagtg aggaaaagca gaaattgttt caggccgagc
    39421 gtgttcctat cgggacgtgt ccccgctgtg gtgaggctgt ctatgagggc aagaaaaact
    39481 actactgcgg aaaccgggcc tgccagttcg tcatgtggaa gaatgaccga ttttttgagg
    39541 aacgcaagaa agcgttcacc ccgaagatcg ccgctgcgct gctcaaaacc ggcaaagtca
    39601 aggtaaaggg cctctattcc gtaaagaccg gcaaaaccta cgacgggacg gtgcttttgg
    39661 ctgataccgg gggcaagtac gtcaactacc gcattgaaca caggagctaa aggacagtaa
    39721 cacagcaagc atagggagtg tggctacact cccggtaaaa tcccccggcc gcgtccctgc
    39781 cgggccgggg atttttgctt gttggggaag tccccaaccc cctatttttt gaaacagaac
    39841 agagaaagga gtgaccgcgt gaacgaagta ttttctatcc aagtccataa ccggcagttt
    39901 ttggaacagg gcaaacaagg cgtctggctg gatttgccca ccacaaccga aacgctgcaa
    39961 gccgccctgc gggatgttgg cattactgcc gacaatccgc aggatttttt tatcaacggg
    40021 tattcctgcc cggaggacag acaccttgcc attccctatg acatggtgct ggccgccgac
    40081 gtggacgagc tgaatttcct tgccgcccgg ctgggccagc ttgacgccgc cgagatcgca
    40141 gagctgaacg ccgccctgca aaatccccgt ggcggctttg agaatatcgg ccagatcatc
    40201 gactacaccg aaaatgtgga ttactatgtg catcttcccg acgttagggg ggatggacag
    40261 ttgggcgact actacctgaa ccgttccggc atggtggata tgccggagga atggaaagcc
    40321 gggattgata cggcgcagtt tggacgccac attgcccagc aggaacaagg cgtttttacc
    40381 cagtacggct atctggtgag aagcggcgac gaatggcagc gcgtccatga gggccagccg
    40441 gtgccggagg aataccgggt gatgcactac ccccagccgg aagctgaccg ggaggaaccg
    40501 caggcccagc agccggaggc atccgaaagc cccgacaaag cgccgccccc ggttatcccg
    40561 atcatgctgg acggcatgaa cagcaaagaa cgcatgaaag tgattactga ccgtctggaa
    40621 accggcgtac aggagctttt tgaaagtagc cgctatatgg cttacttatc cgctatgtcc
    40681 aagatgcacg attacagtct gaacaatacg ctgctgatcg ccatgcaggg cggttcgatt
    40741 gtggcgggct ataacaggtg gcaggatgaa tttgaccgcc atgtgaagcg gggcgagaaa
    40801 ggcattaaga tactggcccc ggccccgttc aaggttaagc agcagatgga gaaaaccgac
    40861 ccggccaccc agcagcccgt gatcggcgcg gacggcaagc ccgtgaccga agaacaggaa
    40921 gtgacggttc cggcctttaa ggtggtgaca gtctttgaca tcagccagac cgagggcaag
    40981 gattttccca tgccctatat cagtgaactt accggcgacg tgcagcagta tcaggatttc
    41041 tttgccgcgc tggaacggac atcaccctat cccataagca ttgagccgat ttcagacggg
    41101 gccaagggcc gctgcttcta cgcaaaacag cgcattgcca tcaacgaggg catgagcgaa
    41161 ttgcagaccc taaagaccgc catccatgag attgcccatg ccaagctcca cgccattgac
    41221 cgggaggccc ccgccgagga acaggcggcc cggccagacc gcagcacccg cgaggtacag
    41281 gccgaaagcg tggcctatac cgtctgccag tattacgggc tggacacgtc ggactattct
    41341 tttggctatg tggccgattg gagcagcggc agggagttat ccgagctgaa agcctccctt
    41401 gaaaccatcc gggccacggc cagcgaattg ataaccgaga tcgacggcca ttttgcccag
    41461 cttcagcagg agcgcgaggc ccggcaggaa caggaggccg tcacggttga cgttaccccg
    41521 gaggcagcgg ccccggcgca ggccgaggaa gaacaggccc cggcccttga tccggcagcc
    41581 gagccgcttg tgactgtgct atggagcgaa agcccccagc taaaggacgg ccagcaaatg
    41641 ccgttgcatg aggccgacgc gctgtttgcc cgtctggatg cagcatatcc ggcagaggca
    41701 ggctatgaca aaaccgcgtt ccgcattgac tgtatctttc agggcgagcc ggacagctac
    41761 gaggggcggc aggattttgg agatagggac ggttccctga tcgaacatat ccagagctac
    41821 caccaatttt acgcgcagga cgaaaactgg aaaaagcatg taatcgctac aaccggcata
    41881 gagggctggg agcaggacaa ggcccatcgg gatatgctgt taggcgagtt tgtcccctat
    41941 atgaagctgc attgcagcct tgcccaaatg gaacagacgg cgcagggccg cttacagtcc
    42001 ggggaaaccc tgacgccgga ggaaacggcc tatttggggg cgcttctctc ttatgtgcag
    42061 gagtgccgcc ccatgctcaa tcagggccag tatcagttgc cggagccgcc gaaactggct
    42121 gacttcgacc agagcttgca ggactacaaa gcacaggtac aggccgagat cgcgcaggag
    42181 gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg ctggctatga agcaccccaa
    42241 caggacacct tttccatcta ccagcttcga ggcggggagg aaacccgcga ctatcggttt
    42301 gagccatacg agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa ctatgacctt
    42361 gtttatacgg caccgctgga taaaagcacg tctttggagg atatttaccg cacattcaac
    42421 atcgaccacc cggcagattt taggggacac agcctttccg tttccgacgt ggtggtgctg
    42481 caccagaatg gacaggacac cgcccactat gtagacagct tcggctatat ggaagtgccg
    42541 gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt ctacggagca gaacgaaaac
    42601 atgattgacg gcattatcaa caatacgccg cccgaaccgc agccggagga acccgaacag
    42661 ctatcttttg aggatgtggc agttgcttcc ggccagacac aggaggaacc cccggaggct
    42721 gcgcccccgc aaacggctgt ccgctattat cccatcaatg agggagccgc ccgccgcgcc
    42781 aaggaagcca tgagcttttc tgactaccgg cccggcagcg ccacggcgga ataccggcac
    42841 tatgtcgatc aggccgtgga aattgcggag cgccagaaaa agcgtgtgga gccagagtat
    42901 cacgaaaaga tagacagcct ccttgacacc tacgcccgca agctggccga gaacatgaat
    42961 aagggctatg agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc gtccaatttc
    43021 ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga ggaatttcag
    43081 tacattcagg gcttgcttga caaaatccgc agcaccggca tgggtggtat cagcgccgac
    43141 gatccgcagg ccgtttccaa gctggaaaag aaactggaaa agctggaagc gtcgcaggag
    43201 ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga cgctggacgg atgcccccat
    43261 ctgatcgaaa gaggcattga aaacctgaaa gcggatatgg cgtcgggctg gcactatgag
    43321 aaaaaaccgt tccagtcctg gcagctctcc aacaataacg ccgagatacg ccggttaaag
    43381 ggccgcattg aggaattgac ccgccaaaag gaggcggcct atgtgggatg ggagtttgac
    43441 ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt tgaggggaag
    43501 ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc gcccaaggcc
    43561 ggggcatggc agcggcagtt aaacgacacc acaatccgcg tcgctgaccg tatcaagtgc
    43621 atccagcccc tttccggcga aaagcccagc gccttacagc ttgcggcccg cagggagaaa
    43681 gaaaagccct ctatccgggc gcagctcaac gcggccaaga ccgaacaggc caagcagcca
    43741 ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt tgaaaatgtg
    43801 gacgttctgg ccgcgctgga acagatcatg cggcagaaca ccgcgtttta tcaaagtgac
    43861 tttggcattg acaaagacat tatcaggcgg gatgccgcca gcgatcaggc cgcagacaag
    43921 accctgctct ggatgtcccg gccctccggg acgtactgtt tccgggaacg tgatgttttc
    43981 ctgaaagaca cccgacagta caacacatgg aaattttacg gggagcagac ccgtgacaag
    44041 atactggcct acgcggtgga gctgaccggc acacagggcg gcactatccg gggcaatctc
    44101 tatgagctgg actatccgca gcatttccgg cacgtcattg aaaccgccca gccggtgagt
    44161 gtgaaccggc tcttttatga gcatggcacc cgtgacatcc cggagagcca gcgttttgac
    44221 ggaagccctg accgggtatt gggaaacttt ttgtactatg aggcccagcc ccatgacccg
    44281 gctgtgctgc aggaggcgct acggacggag cagcacagcc gggggcgggc cgtccccggc
    44341 gatttcaaag tccacgtcac cgctttgcat gacagccgga ttgaaaccga ggcccggcgt
    44401 attgtggaca gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac gcatttcatg
    44461 gtggagatct ccccgcactt tgcggcgatt gcttccacca aggataatga ccggctcttt
    44521 gccatgctgc cctataaatc cctgtgtttt accgggatga aaggcaggca tggcgtgtat
    44581 gccgtgatta accgggacga gcgccgggat gtgagtattc gcaggccccg gccctctatc
    44641 cgcaagcagc tatccgacag caaacaggcc gccagcccaa aaaaggcggc ggccagaacc
    44701 aaaaagaatg aattggaggt ataaggaatg atgcacttta ccgtggagga agaaaacctg
    44761 atctgtatgt accacaatgc agaccggcgc agaacgataa acaggctcac cgccgtcatg
    44821 ccggttatgg atggggatat gcgggcgcag gcccagcaga ccctttccaa gctggaacgc
    44881 atgacggatg ccgactatga cggccagaag ttccacttca cagacgaata acgaacagca
    44941 gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt
    45001 cctacgcatt tagaacccgg tattgtcggg gataggatga agtattcgct tgcccctgca
    45061 aacatgcggg agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag
    45121 ctataattag acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg
    45181 tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt
    45241 tccagcggga agctcggcaa tgcttccagc agtttcaggg aaatgcgctg ccgtatatct
    45301 tcgtcaatga ctttcttctt tttgccgttt gcctgtctga tctccaccgt ggaaagctcg
    45361 ttgatgtagt cagcataatg ggccaacact ttttccgtgg cccacctctc accggcaacg
    45421 gcggctttga tcgtttcata gtccagcagc ttttcttgtc cgtccatcct gatacccccg
    45481 tcagactgat tttttctatg ataccatatt cggaagcatg gaaccagaga aaacggcgct
    45541 tttctgctgg taaattctac gcatttagaa cagcacttcg gcggggatag gataaaacac
    45601 ccacataccc cgctgaccat gccggaaaag ccttgaaata taagggcttt tacagctcta
    45661 agtgcgtagg tttccagtat cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc
    45721 gtttggagca ggccgcgcag tatttggccc ggttagactt cggcgtgaac gtggccccgc
    45781 actcacagca gcgtttcact tcgtcccggt ctttggtgat ctcggcatac agggccgcat
    45841 ccagcgggag gacagcggcc ttaaaccagc ggcacagaag cgaataggaa atgctctgta
    45901 cgcaaataca ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa tagttgcagc
    45961 actcatgcgt cagcttccgg gccttgcgat attgagggta attcattcgt ggaatactca
    46021 tagctcttgc gtccattcct ttcccggcgt catacgcaaa atgccgtcca cattctgctt
    46081 gatgatgccg tattcctgca tctgcttttt cgcttcgccg tacttctcat acagccgttc
    46141 cttttcctcg gccaatcgct tgtactcggc tttcagggcg tacagatcgg ggagcttttt
    46201 cacaccctgt ccctttaggg cctttgccgc agcttcatac aaaatcaacg tgctttcatg
    46261 ctcacgccgg aattttgcct tatccgctgc attgcggtat tccatagcca cgggccagag
    46321 ctgcttgtag gtagaaatat tctttatcag caccgccata tccgcaagcc ggtgttccat
    46381 agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca ctttgctttc
    46441 caaatccggg taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg
    46501 cttcaaattg tggagcttcg cccacctctc atagcctgcc gactgctggg ccttaatgtt
    46561 gttttccaga tcaatccgca aagaaatccc cttgcgctct ttgggcgctc tggtgataac
    46621 gcggcctttg atacgctctt tgatggcttc ctcggtgtag gcttccccca atgtcttgca
    46681 gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata aactttcccg gcttgatctc
    46741 atagccctgc gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc
    46801 ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc cctttccgct gggcgtccca
    46861 ctcggcatag ctcttgccct tactctggcc gggcttcacc acggacaatc cgttttcctt
    46921 gcacagcctg tcgctggtgc gccggatata ggcatagctc tgtttgttgg aaatgtactt
    46981 gcgctgattt accatatcca cggcacagaa aataatgtga ttgtggacgt ggcccttatc
    47041 aatatgggta gtcagcacat agggatattt cccttgcagc acttcctcgg caagctgctt
    47101 cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg
    47161 gtggcccaga ttattgccct tttggtatgc ctgcgccagc agcatttcaa attcaatgtc
    47221 cgctgtttca taagagcagg caaaggaggc caccagcagc ttgccgtcag ttttgtccgg
    47281 gttctcgatg tagtcaaggg cttttttcag cgttgacttg ataggatgta tctttgtaac
    47341 tgccatatct gcgccagcgc cccctttata tcctcgatgt cctgtgcgta caccggcccc
    47401 ataccgttca gcctctttgc aatctgattg acgttgacgc cgatcttctg tatctcggcg
    47461 gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat gagcatcttc
    47521 cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc aatcatagcc
    47581 cgttcctccg gcgtcacagg gacgcgcagc acgatttttc gctttctgtt tgccatgctt
    47641 caccgtcctt tccattcagg ggtttgggga tttccccaac aagcaaaacg gacgcggccc
    47701 ggcagggacg gggcaggccg tttttccggg agtggctaca ctccctatgc ttgctacgtt
    47761 actgtttctc cactaacaag acgtttccaa agccccaaac tacccgcaaa atagccctct
    47821 ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa
    47881 cagaggatgc cagttacaca tcctccgttt cattcttctt cggcttttca attagtatgg
    47941 cgctgtcaat cgcgccctcg atgatcggca ggtattcttc ggggcagagt ttcagcttgt
    48001 ggctgaccct ttgccgcagt tcgctttcct cccgcatgat ctccgggtta aagtagcgtt
    48061 ccacaggaag cccgcaggct ttaataagct gtatgacaac cggcagactg ggaatggccc
    48121 gtcgttttcg atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt
    48181 gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc
    48241 gggacaatct tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc
    48301 tgcccgtgga atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct
    48361 tttattcttc gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg
    48421 acgttgaaag aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt
    48481 gctgcgggac gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact
    48541 gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat
    48601 gaaaccggcg cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg
    48661 aaaaactata agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg
    48721 aaacagaaag aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga
    48781 aatggaaaat gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag
    48841 aattgaaaag gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc
    48901 tggtctaatg cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt
    48961 ttgaggaaaa tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg
    49021 acacggatga tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt
    49081 attaggactg aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg
    49141 ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt
    49201 tgctgaggct tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt
    49261 ggatcagaat atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt
    49321 ttgacaaccg ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct
    49381 tgatttaatt cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc
    49441 ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag
    49501 actgttatgt tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc
    49561 gaggatgatg atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag
    49621 gtggatgcct gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa
    49681 ctggttattc ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt
    49741 cgtgcgaaaa atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa
    49801 atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt
    49861 ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt
    49921 gtcggcaggc tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg
    49981 cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc
    50041 ttaacgcatg aaatcatttt gtcacgtata tggggatatg actttgaggg tgatggcagc
    50101 acagtccaca ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc
    50161 attcgcggtg taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca
    50221 ttaaggtttt ttcctatacg atcattgtcc tgctactgct tgtcggtgta acagcaacac
    50281 tgtttgcaca gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat
    50341 cctatcagcc attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg
    50401 cagggctgtt tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa
    50461 gcgtactcta tgccacacca aatgccaata catcaaatag ttttagaccc aactttcttt
    50521 atgtggtaca tagagatgat aatatttcga ttgttgctca aagcaaggca ggtgtgggat
    50581 tgctttatca agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca
    50641 gccttttatg cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg
    50701 acagtgcgaa taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg
    50761 agcttggcgc actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg
    50821 caaggctgga ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct
    50881 ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg
    50941 gaatgcttga aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca
    51001 aaatgatgga taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg
    51061 atgggagaat cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc
    51121 tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag
    51181 ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat
    51241 tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg
    51301 ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc
    51361 caaggctgtt caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa
    51421 gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt acaaaaaacg ctggatgcca
    51481 tgagcctcca gtatgcgctg gaaaacacct cggatggcgt tttgttctgg ctggatttac
    51541 cgctcacatc aacattgtaa atatttaaaa cttaaatgat tt
//
LOCUS       DAYYGY010000010        51202 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000010 DAYYGY010000000
VERSION     DAYYGY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.010"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..406)
                     /locus_tag="ACHA52_000626"
     CDS             complement(101..406)
                     /locus_tag="ACHA52_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939378.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(403..564)
                     /locus_tag="ACHA52_000627"
     CDS             complement(403..564)
                     /locus_tag="ACHA52_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8939379.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(561..863)
                     /locus_tag="ACHA52_000628"
     CDS             complement(561..863)
                     /locus_tag="ACHA52_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939380.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(856..1020)
                     /locus_tag="ACHA52_000629"
     CDS             complement(856..1020)
                     /locus_tag="ACHA52_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939381.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(1025..1294)
                     /locus_tag="ACHA52_000630"
     CDS             complement(1025..1294)
                     /locus_tag="ACHA52_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939382.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(1306..2055)
                     /locus_tag="ACHA52_000631"
     CDS             complement(1306..2055)
                     /locus_tag="ACHA52_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939383.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(2058..2744)
                     /locus_tag="ACHA52_000632"
     CDS             complement(2058..2744)
                     /locus_tag="ACHA52_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8939384.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(2750..3421)
                     /locus_tag="ACHA52_000633"
     CDS             complement(2750..3421)
                     /locus_tag="ACHA52_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8939385.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(3414..3755)
                     /locus_tag="ACHA52_000634"
     CDS             complement(3414..3755)
                     /locus_tag="ACHA52_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939386.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(3933..4631)
                     /locus_tag="ACHA52_000635"
     CDS             complement(3933..4631)
                     /locus_tag="ACHA52_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8939387.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4718..5188
                     /locus_tag="ACHA52_000636"
     CDS             4718..5188
                     /locus_tag="ACHA52_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939388.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(5231..5410)
                     /locus_tag="ACHA52_000637"
     CDS             complement(5231..5410)
                     /locus_tag="ACHA52_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939389.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(5397..5735)
                     /locus_tag="ACHA52_000638"
     CDS             complement(5397..5735)
                     /locus_tag="ACHA52_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8939390.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(5751..5954)
                     /locus_tag="ACHA52_000639"
     CDS             complement(5751..5954)
                     /locus_tag="ACHA52_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939391.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(5969..6745)
                     /locus_tag="ACHA52_000640"
     CDS             complement(5969..6745)
                     /locus_tag="ACHA52_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8939392.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            7044..7364
                     /locus_tag="ACHA52_000641"
     CDS             7044..7364
                     /locus_tag="ACHA52_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8939393.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            7382..7813
                     /locus_tag="ACHA52_000642"
     CDS             7382..7813
                     /locus_tag="ACHA52_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8939394.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            7943..8404
                     /locus_tag="ACHA52_000643"
     CDS             7943..8404
                     /locus_tag="ACHA52_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8939395.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            8438..9073
                     /locus_tag="ACHA52_000644"
     CDS             8438..9073
                     /locus_tag="ACHA52_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8939396.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            9186..9821
                     /locus_tag="ACHA52_000645"
     CDS             9186..9821
                     /locus_tag="ACHA52_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939397.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(9814..9948)
                     /locus_tag="ACHA52_000646"
     CDS             complement(9814..9948)
                     /locus_tag="ACHA52_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939398.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            10120..11259
                     /locus_tag="ACHA52_000647"
     CDS             10120..11259
                     /locus_tag="ACHA52_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8939399.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(11328..11401)
                     /locus_tag="ACHA52_000648"
     tRNA            complement(11328..11401)
                     /locus_tag="ACHA52_000648"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11365..11367),aa:Arg,seq:cct)
     gene            11555..11719
                     /locus_tag="ACHA52_000649"
     CDS             11555..11719
                     /locus_tag="ACHA52_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939400.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            complement(11790..12970)
                     /gene="eis"
                     /locus_tag="ACHA52_000650"
                     /pseudo
     CDS             complement(11790..12970)
                     /gene="eis"
                     /locus_tag="ACHA52_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced intracellular survival protein Eis"
     gene            complement(13093..13506)
                     /locus_tag="ACHA52_000651"
     CDS             complement(13093..13506)
                     /locus_tag="ACHA52_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8939401.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            complement(13506..13688)
                     /locus_tag="ACHA52_000652"
     CDS             complement(13506..13688)
                     /locus_tag="ACHA52_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8939402.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            complement(13719..13940)
                     /locus_tag="ACHA52_000653"
     CDS             complement(13719..13940)
                     /locus_tag="ACHA52_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939403.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            complement(14008..15468)
                     /locus_tag="ACHA52_000654"
     CDS             complement(14008..15468)
                     /locus_tag="ACHA52_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8939404.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            complement(15576..15896)
                     /locus_tag="ACHA52_000655"
     CDS             complement(15576..15896)
                     /locus_tag="ACHA52_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8939405.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            complement(15926..16243)
                     /locus_tag="ACHA52_000656"
     CDS             complement(15926..16243)
                     /locus_tag="ACHA52_000656"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8939406.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            complement(16395..19097)
                     /locus_tag="ACHA52_000657"
     CDS             complement(16395..19097)
                     /locus_tag="ACHA52_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8939407.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            complement(19160..20437)
                     /locus_tag="ACHA52_000658"
     CDS             complement(19160..20437)
                     /locus_tag="ACHA52_000658"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8939408.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            complement(20437..21450)
                     /locus_tag="ACHA52_000659"
     CDS             complement(20437..21450)
                     /locus_tag="ACHA52_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8939409.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            complement(21466..22194)
                     /gene="sfsA"
                     /locus_tag="ACHA52_000660"
     CDS             complement(21466..22194)
                     /gene="sfsA"
                     /locus_tag="ACHA52_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8939410.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            22371..22700
                     /locus_tag="ACHA52_000661"
     CDS             22371..22700
                     /locus_tag="ACHA52_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8939411.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22884..23531
                     /locus_tag="ACHA52_000662"
     CDS             22884..23531
                     /locus_tag="ACHA52_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8939412.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            23546..25072
                     /gene="zwf"
                     /locus_tag="ACHA52_000663"
     CDS             23546..25072
                     /gene="zwf"
                     /locus_tag="ACHA52_000663"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8939413.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(25121..27919)
                     /gene="ileS"
                     /locus_tag="ACHA52_000664"
     CDS             complement(25121..27919)
                     /gene="ileS"
                     /locus_tag="ACHA52_000664"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8939414.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28181..28888)
                     /locus_tag="ACHA52_000665"
     CDS             complement(28181..28888)
                     /locus_tag="ACHA52_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8939415.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(28909..29691)
                     /locus_tag="ACHA52_000666"
     CDS             complement(28909..29691)
                     /locus_tag="ACHA52_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8939416.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(29740..29961)
                     /locus_tag="ACHA52_000667"
     CDS             complement(29740..29961)
                     /locus_tag="ACHA52_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8939417.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(30042..30641)
                     /locus_tag="ACHA52_000668"
     CDS             complement(30042..30641)
                     /locus_tag="ACHA52_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8939418.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(30655..31332)
                     /locus_tag="ACHA52_000669"
     CDS             complement(30655..31332)
                     /locus_tag="ACHA52_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8939419.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(31349..32590)
                     /gene="ftsZ"
                     /locus_tag="ACHA52_000670"
     CDS             complement(31349..32590)
                     /gene="ftsZ"
                     /locus_tag="ACHA52_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8939420.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(32613..33938)
                     /gene="ftsA"
                     /locus_tag="ACHA52_000671"
     CDS             complement(32613..33938)
                     /gene="ftsA"
                     /locus_tag="ACHA52_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8939421.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(34094..35314)
                     /locus_tag="ACHA52_000672"
     CDS             complement(34094..35314)
                     /locus_tag="ACHA52_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8939422.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(35329..36417)
                     /gene="murG"
                     /locus_tag="ACHA52_000673"
     CDS             complement(35329..36417)
                     /gene="murG"
                     /locus_tag="ACHA52_000673"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8939423.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(36443..37804)
                     /gene="murD"
                     /locus_tag="ACHA52_000674"
     CDS             complement(36443..37804)
                     /gene="murD"
                     /locus_tag="ACHA52_000674"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8939424.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYRHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(37818..38780)
                     /gene="mraY"
                     /locus_tag="ACHA52_000675"
     CDS             complement(37818..38780)
                     /gene="mraY"
                     /locus_tag="ACHA52_000675"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8939425.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(38806..40998)
                     /locus_tag="ACHA52_000676"
     CDS             complement(38806..40998)
                     /locus_tag="ACHA52_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8939426.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(40999..41400)
                     /gene="ftsL"
                     /locus_tag="ACHA52_000677"
     CDS             complement(40999..41400)
                     /gene="ftsL"
                     /locus_tag="ACHA52_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8939427.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(41405..42364)
                     /gene="rsmH"
                     /locus_tag="ACHA52_000678"
     CDS             complement(41405..42364)
                     /gene="rsmH"
                     /locus_tag="ACHA52_000678"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8939428.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(42385..42816)
                     /gene="mraZ"
                     /locus_tag="ACHA52_000679"
     CDS             complement(42385..42816)
                     /gene="mraZ"
                     /locus_tag="ACHA52_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8939429.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(42986..43354)
                     /locus_tag="ACHA52_000680"
     CDS             complement(42986..43354)
                     /locus_tag="ACHA52_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8939430.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            43525..44481
                     /locus_tag="ACHA52_000681"
     CDS             43525..44481
                     /locus_tag="ACHA52_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8939431.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            44554..44670
                     /locus_tag="ACHA52_000682"
     CDS             44554..44670
                     /locus_tag="ACHA52_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8939432.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(44725..46407)
                     /gene="recN"
                     /locus_tag="ACHA52_000683"
     CDS             complement(44725..46407)
                     /gene="recN"
                     /locus_tag="ACHA52_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8939433.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(46425..46874)
                     /locus_tag="ACHA52_000684"
     CDS             complement(46425..46874)
                     /locus_tag="ACHA52_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8939434.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(46981..47799)
                     /locus_tag="ACHA52_000685"
     CDS             complement(46981..47799)
                     /locus_tag="ACHA52_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8939435.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(47808..48698)
                     /locus_tag="ACHA52_000686"
     CDS             complement(47808..48698)
                     /locus_tag="ACHA52_000686"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8939436.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(48700..48924)
                     /locus_tag="ACHA52_000687"
     CDS             complement(48700..48924)
                     /locus_tag="ACHA52_000687"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8939437.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(48929..50266)
                     /gene="xseA"
                     /locus_tag="ACHA52_000688"
     CDS             complement(48929..50266)
                     /gene="xseA"
                     /locus_tag="ACHA52_000688"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8939438.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(50267..51121)
                     /locus_tag="ACHA52_000689"
     CDS             complement(50267..51121)
                     /locus_tag="ACHA52_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8939439.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
BASE COUNT        14703 a        10633 c         8598 g        17268 t
ORIGIN      
        1 aacttaacta attcagctcg ttcttttgat tttttatctt tctgaatctt catcatgatt
       61 ttcttgagga tgatgtcact gtattgtgta atgagatcca ttatctctcc tccacatacc
      121 taaactgtcg tccttttgaa tcaatccata agctcctagc tctatcccag ataatgtttt
      181 tgcttaatcc agtaatttca gataactgtt cagcagtacc tgttactaaa attcggtcat
      241 catgccagat tgcaattttt ctcggcgttc tccgtttggg cttttcagtc cacattgatt
      301 taccgagctt ttggacttct gcaactattt ctttgtcttc ttgccaagat tctgacttgg
      361 ttaattcagc aattcttttc attgctgctt tcttatccac gctcattcct ccaatcgatg
      421 gatttccctt cttaaattct ctatgtgcaa atcgattgcc tttctcgccg tttcattgac
      481 catcactgcc tttgttcgtt ccagatcgtc aatctcacgc tgaaggcttc gaatacgcat
      541 ttgaatcact tcttctgttg tcatgatgat tcctccacgt atctaaacgt tctcttctta
      601 gcgtctgtgt agccacacct tgctcttttc ctcacgattt tctcgtgcaa gcctgtgaga
      661 cttgctaact gttcagctgt ccctatcact agaagtttgt caccgtgcca aattgcgatt
      721 tttcgtggtc ttggcttgtt gctcttgtct gcccacatcg ctcttccaag cctcatcact
      781 tctgaagcag cttctttgtc attttgccaa tcttctgaat aagtcaattc gataattcgc
      841 tgcattgccg ctttcttatc catcccgaca ttctcctttc agtaatttga gtacttgatc
      901 aagtgcgctc tcacgtccgc catggaacgt gttgagccac ttgtcttcgt acgatacact
      961 ttgtcttaaa gcttcttgat gcattagttc aatctgtgct gtaaatgtct ttaggtccat
     1021 ctgattacac ctgctcaagt tcattaagat gtttttgcaa gcctttaaca caatcaacaa
     1081 atagtaattt ggtatatgct aaatttctta attgtgttgt atcgatataa agtgcgaaat
     1141 agtatctgag cttactccaa ctcgaacgat cattcttaat tccttcaatt ccagcttctt
     1201 cgagttgatc atatacgtct ctcaggacgt ctaattcctc gcccttttta taattcgcta
     1261 tttcattaat tagttctaga taatcgatct tcatttttcc acctcttaga atggtgcttt
     1321 tgattgtcta ttagctcgtt ctagcgcttt tttcttgaga taggcttctt ggtcgattgc
     1381 ccattcagga agcttctctc gtcttcctgt gcgcttgtat ccactgcttg cgttcttagg
     1441 ttcacttttt tctttccttg cccaacttcg aatagttgcc aaatagtttt tataagtctt
     1501 accagatgat tcacaatact ctgacagtcg ttcgattcgc tcttggtaat cattagggaa
     1561 ttctgttttg agtttctcca tctgctcatc tgacaaaaga acatttttat actctccgta
     1621 tttatgacgg acgggcttag ccttcgattt tttcgaaggc ggtaactctc ttatatattc
     1681 ttttgtatta ttaaatgtat tattaataga tgtattatta tctttgactt tttcgtcaat
     1741 aggggtattg cgtttttcgt caatagggta ttgattaatt cgtaggtacc tattgattat
     1801 ttgattggta ccctctttgt aaatgatttc ccgattcaag tatccaaact taatcaaatc
     1861 acttacccat cgcgatatgg tctctttatt cacaccatat aaatctgcaa agtactcatt
     1921 gcctgcccaa caaaagcctc tttcattaca caaggccgtt atctctccgt ataacaactt
     1981 agtatttggt ttaagtcttt tgtcgtacct tacgttggct ggtataatcg cataataact
     2041 tcgatgttct gtcattttta ccctccaata tttaactttt tgattgtttt ctggtttaat
     2101 ttgatccctt tgatttgata tttatttttg aaattaatca cacctatttt gtgcttctct
     2161 gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg catgatcaac ttctttgcgc
     2221 tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag ctcctgtttt gccacagatg
     2281 cagcagactc tttttgtgat gcatttgtag aagtaatatt cttgattcgc aggtaaaatc
     2341 tcatagcctt ctttgaaagg aatatgatgt tcaaagatga aatctaagat gatatttgct
     2401 aagacattgg catcactcac agttgtattc gattcgtctt tcaggcttat tttgcgccct
     2461 gtgacacctt caaaacgaaa gtagaagaat tccttccaga agtccgttgg catgcctgta
     2521 tcgataaaaa tatcgcctat cagcgcatag atgaagtttc gttgctgtac ggtgaaacgt
     2581 ctaggatcaa taaaacgaat ttcaatgact cgatcaccat cgtagccgtc atacatcgtc
     2641 tttagtcgat caatgttcac ttcctcattg atggttgcgc ttatgtcttt tcctttgaac
     2701 tttttcagaa ccgctgaata tgaatcgatt aatggtttaa acactcatat cacttcttat
     2761 ctaattcttt tctcttagct gctattgctc gctccatcaa ggcacattgc tcatagctta
     2821 actgttcaat agtttcaacg ttatcagcta agagccctaa tttatctgtc tgctcattaa
     2881 catattcgat taaggttttg gtcatatctt tacccatctg ctcattgaaa gcttctagaa
     2941 tcgtctctag catgtttaat ttctttgtat cgattctagg tggtgttgga atatcttccc
     3001 cttgaaatac atataatccc agtccgtgta gagccaatgc tttcacaaag catcgcttca
     3061 atgagttatt gatttgcatt gcatttggtt taacaactgg ttggtttcga taatctaaaa
     3121 caggaaataa ttcggtttcc gtgtgtcctt taaccgttac tgagacagat acataagtcc
     3181 cagtttcatc cataagaaaa ggtttatatt cctcaacaag aaagtcttga tgagttccag
     3241 aaacaaccct gtagtgtttg tactcattaa tagttaccgt tgcctgtgga tcattctttt
     3301 tcataatctc ccacgcgtga gcccaagata aataatcaaa atttcctttt ttcttgagaa
     3361 ttttatttaa cttacgacta aaaagttttt caaagttcgt tgtccctttg atttcactca
     3421 tcaaattctg cctccatttc agcaatgtat ttcttacctg gtccgtaata agagatatca
     3481 atcaagttat ctctgtcgta ctcttctagc gcatcaatca agccatcttc gatgacgtaa
     3541 atatattcag gtttattcga atgcttcgat agatggataa gataaacatg atcccaaata
     3601 gttacatggt tgcctaaatc atcttgatcc caagctagtt cttcattcgt caaaagattt
     3661 cgtctgattt ttcgaccact tgtttcctca attttcggct tgccccaatc aggatcaatc
     3721 aaatattgat ctagagtgga aagttctttt tccatatgct aaaatctcct tatgatgtgt
     3781 tttctttgtg actctttgct tgccggcgga gtcacttttt tatttgttgc catgcttttt
     3841 gcttgtcaat atgttgttgg cttaggatga ttggtttata gtatttccac cagcaattag
     3901 caattgccgt ccctattctt agcgcttcag ctctattcat tgtcatcacc aaaaagtctc
     3961 tgttgtctgt tcagttgatc aatttccata cggatcgcag tttctggcaa ccacatttca
     4021 ataaatgaaa cagcatcatc gaatctctta cgaggtaact cgccatatct tgggattgaa
     4081 aaggtacgtt taaattcaga ccaaaatttt gagaatactt ttttgctgat ttcttcataa
     4141 gctcggcttt cttttccccc tagaactccc ataactttca tatttccttt ttgcttaatt
     4201 tcaaactctt gttgtccgct aattcgcata gtatctttaa gcatggaaac atctttttta
     4261 acatctttca tttcttctaa ttgatagatc atcatatctt caattgtttg aggaacagta
     4321 ttcttgcgaa taacatcttc catttcgttg aatgcttcaa tgtatttttg tttgaagtag
     4381 atagctttct ttcctgtaaa ccccatagcc agcaagaaaa atccatctct actaatgaag
     4441 aaaactcgtc gatttctgcc atatgaatct ggttcattac cttctacaaa catctgtcca
     4501 aaattggaca catctttttt tagtgcatca atatctctta aaacatgttg atgttttttc
     4561 tcgaagcttt ctgccacttg taagctcgta gtcacagctt ctttattttt caaaattact
     4621 aattcttgca ttatttcttc tctccttttt ggtataattt agttaaaaac tggtggtgtt
     4681 tattttgaat ttttttcact taacaatttt tcaatggatg tcaataataa gttttatatt
     4741 gagtacgttt ttaacaggga taaaaataaa aggaatgcgc cctcaactag aagttgaact
     4801 taatgcttct tacttcgctg cagatatgat ttatacaaaa gtaatcattt ctaactattc
     4861 aacagaacca gctatgttag ttaatttaga actttcatcc gctaacttaa accgcagatg
     4921 gtcagctact ccatttaaga aattaattgc taaaggagga tcagtcgatg ataatagaat
     4981 atactctgaa gctgtaccgc taaatattcc tcctaaaagt gctatatctt gttaccttgc
     5041 atttgaagta gggaaaatta attttaataa actccttaat caccaaacaa aaatgatatt
     5101 tactctaaat agaacccaaa tccacaaaat tgttgatatc aaaaatacaa acttcccaat
     5161 agagaaatta gtgaaagaat taaactgagt aatagtaata tacattctag tttctctttt
     5221 ctattcattt ttagtcagcc ccctcggttg gctttttcgc tctgtactca gcttcagctt
     5281 catcaagccc cataaaaatc caaaccatgt aaacaatcgt tcctatcaac gcttgcctgc
     5341 ttccccaaag ttctaaagcg tagatgatta gcggtgcgct gaacactagc gctctattaa
     5401 attttcccat ccgcttgcct ccttaagatt tccgaaaaat ttgtttctaa aaattcaagt
     5461 gttttacttc ttaaaaatag atatgtgtct cttccttcaa ctggataata gacaaatcca
     5521 tttttgtttt tctcgatatc gataatattt ctatatcttg ggttctttaa aactctagaa
     5581 gtaaaccaat catattttct gttaatccgt tctagcactt ctggcaacgt catccatcta
     5641 ccagtatcat cagctttttt taactcctca taatccactt gggagataat tacatagcct
     5701 tctggaattg ggatttttgc ttctagatat tgcattagct gttcctccct ttacaattcg
     5761 tacatagtga taatcgaatc tataattctg tttgcttctg cagaagtctt tttaccgttt
     5821 aaaattaaag ataagtagct tttgctaatt ccaaatcttt cagcaagcat ggtgtaagtt
     5881 aagaactttg aactttcgac atattctttg attttttctc tatctcgttg agtgatttct
     5941 gcaatgtcag tcatactaaa actcctttct aaccaatttc ctctaaatcc atttgagggt
     6001 aatatccttc tttttttagt aattcgtaga taaatagacg ccctttctgt gtccatttgg
     6061 tattcattac aattttagtg ccaccatcgg ctttcgggat ctcagttgta tgagattttg
     6121 tgtatccttg gttcatgtgt tttttgcaca ataaccattg gttaccgact tttttctgaa
     6181 tacctagttt atgaagtaat ttattcatct gttgtggaga catcccataa tctgctgcaa
     6241 tctgactaat tgttactgaa tctgtagaag ataatatgct atctaaatag gagattttag
     6301 gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc agttctttct tcttttagtt
     6361 gagttgctaa ttgaatgatt gtatctggat taagcaaagc ttcttctact ttttctggag
     6421 ttagataacc tccatgtttt ctaattgctg gcaacacttc acttgttacc caacgtttaa
     6481 attttttggc agagggaagt tttgatttta agattaaact gtacaaacca gattcgttga
     6541 tgattgtcat ctctcttgat tgacctgagg tcgtgattcg cgaccccatc ttatcttcca
     6601 aatctacatg ccgcgacaaa gcatctttag tgtttgaata acccaaaaca ctcgcaacgt
     6661 ctttgcctac aaaatatggt tcatcattta ctaaaattgt ccgaacttcg ttttgttcga
     6721 aattaaaaat ttgcggtgtg ttcatatttc tcattccttt cttttatatt cgtaaacaaa
     6781 tttaacaact tttttctaaa ttcaattgac agtatctgag taatactcta tactaagagc
     6841 ataattaaat aagacataaa aacattgatt ttaaagcttt cttggcggtc tgcatttaca
     6901 tatcaatagt gttttttgtt gtctttttag ttgttaaatc tgtttacaag atatagtata
     6961 gctcattact cagaattagt caaattattt ctgcgtattt ttctaaactt tttttgtaaa
     7021 catttagaaa ggttgtttta tcgatgaata cctatgaaat aataaaagac ttggcgaaga
     7081 ggaaaaagat atctatacgg cagttagaaa ttaattttgg atattctaac ggttatctag
     7141 caaagtggaa aaataatact cctaattcag atgaattaag tcggattgct gactatttcg
     7201 atgtctctgt agactacctt ctaggtagag aagaaagaga aacccctaaa catgtggatt
     7261 tatcagaaga cgatactgtt ttttcttttg atggaaaaga aatatctaag gagacaatgc
     7321 gtaaagcgat tgcaattgct aaagctttag aggaaaatga atagttggag tgatggtttg
     7381 tatgtatttg aaattgaaag aaatgttgag cgaatacaat ttgaaagtag tttatatgga
     7441 gatgaaagag cctggtttct actatccaaa acccagaatc atttttttga atgaaaatct
     7501 atacggtgaa actgcagaag cctttcattt atctcatgaa cttgcacatt tcagcgcctc
     7561 tcattttgaa ttttcagttt tgtacgatac ttctacaact tttcactcaa aatttgaaac
     7621 agaagcagat aaaatagcta ttctaatttt attaaatatc tatattgaga acgaactgac
     7681 tgatgaatct cagtttaatc tcgaaaaatt tatggagtat tattctattc agaataagct
     7741 cagatacact tgttatgctg tctgccaatg ctattttaag aaaaaatatt cttacgcaag
     7801 gcaatatgta tgaatacatc aagaattatt aaaaaaaccg atttcagcaa aacattaaaa
     7861 aagcccgtgc tgcaacacgg gctctttcct catatatgag cttctacaaa aaaaatcata
     7921 tcacagaaat gaggaagaaa aaatggaaaa agagatttta ataaaatctc caggaaaaac
     7981 cttgattaaa gtaactgatg attctatttc aattattaga aaaggattta taaatttagt
     8041 taatcaaggt attaaaggag aaaaaactat cccctttaaa aatatatcag ccgtacaatt
     8101 aaaaaaacca ggtatgagta atggttatat acaatttacc ttgctaggcg gaaacgaaag
     8161 tcgcggaggt atattagcgg ccacgaaaga tgaaaacacc gttatgttta caaaaaaata
     8221 ttggaatgaa atggaaaatt taaaaaaata tatagagaag caacaatcta tattagataa
     8281 taattccaaa gaaacacaaa tttcaagtgc agatgaaatt aaaaagtata aagagttgtt
     8341 agatgaaggg attattaatc aagaagaatt cgattttaag aaaaaagaat tgcttggttt
     8401 ataatattca atatcaaaag agaaagagga tctattaatg gacggtatat ttggttttac
     8461 tggtattgtt ttatttatta taggtttaat catgttaatt gtaagattta tcaaaaaaac
     8521 aaataagaaa acacctatga ttctccttat tatcgggatt atttttacta ccgtaggttt
     8581 ctcgctttcc tcatcaaacg aggcaaatga caataacaaa caggaaagct catcatctga
     8641 tactcaaact actacatcta acagcagcaa taaaagccta actcaatttg aagaattttt
     8701 agagacaaat aatcatgact ggggtgcttt cttagatagt tattattcta ttactccagc
     8761 tactgagcaa aatacagctt ttactaaata tattagtggt aaaacatata cttttgaagg
     8821 aactgttatc gaatcaatga ctactagaat agccattata gcagacaaag agtacgatga
     8881 taaaagttgg aatgatattt ctgcaacacc taaagtttca tatgtaattt ttgcgaagga
     8941 cttaaacaat gctgatacct ttacaaaggg cgataaagta acttttacag gagaaattag
     9001 ttcaagagga tcgaacatag aaaaatccta tgctcaatgg gatatgatta atagtaaagt
     9061 aagtaaaaaa taaatgaacc acgccccact aagagttaga ttgtgcacaa taataaaacc
     9121 tatacaaaag agtgccatca ggagtcacaa tgcttgtaaa ggaaatgaca aaataggagg
     9181 taactatgga taatagcaat gaaaaactaa ccaaaaaaga atatcttgaa ttaatagttg
     9241 aaggcggtat tggagcaatt ccttatattg gtggcgttgt gcagtcactt tattttgggt
     9301 ctaaaaacga gaagcgtttc aaacgcatcg agaaattcta caaaaattta aacgaatcat
     9361 tagaaaacat tcgcgatcaa attccagaag atgttttcaa cttagagaac aaggaccaac
     9421 tcattggaat attcgaaggg ataaatgacg aggtagaaaa atcaaaggca caaaataaaa
     9481 tagatatgta taaaaatctt tataagaatt gtttacttcg aattaacaac gcttcatggg
     9541 ataacgagga gtattatctt cttgtactga atcaattaac atcgattgag attcaattgt
     9601 tagcatgctt aatgagtcgt ggaaacgaaa atttcactgg aaatatcagc atgtctggct
     9661 attcacaaga gctaatagat ggtagcttaa atagattatc tgattttggt ctgttagaaa
     9721 aaacgattag ctccatagta ttaggtgatg tcggcaaaca aaatatgggc tacaaaatat
     9781 ctcatttagg aattcaattt attaactcaa ctcttactta agttttcttt ctaatatgac
     9841 atctagacct atcagcaatg caacaataaa aaaccagaca taccaataat caacaaataa
     9901 tctaaactct ggaagttcag ttattctttt aaaaatcact ttaatcataa taaacacctc
     9961 tgattggatg atctaataca catccaatta taccattaaa acgacaaata gcacatcctt
    10021 gcgccggcaa gcaaaaagga tgtgcaaaaa ataataaaac ccaaacaatg ggcttcttta
    10081 tagttcctat tgtatcagag aaagagagct gattcaatta tggcaagatt agtcaaacgt
    10141 ggaaatagtt ggcaatacga aatttcatac aaaaaagatg acggaaaata cacgaagata
    10201 agaaaatcag gatttaagac aaaaggagaa gcaaaagatg ccgccaacga attagaatat
    10261 aacttgaaca aaggccttaa aggggatcgc aaaaatctat tattatcaga ttactttgag
    10321 gattggatgc aactttataa agaaggaaca gtatctccta tcacttatag aaaatacgaa
    10381 gatacgttaa tgaacataaa gaaatatatg ccagcggtat tgatttctga tttagataga
    10441 gtgggatatc aacgcttttt aaataagtat gctaaagacc atgtaaaatc taccgttatt
    10501 aagtttaata accatattag agcgtcgttg aaagatgccg tagaagaagg attaattccg
    10561 tttgatccaa ctagaaaagc agtaatcaaa ggaaaagatt cattgaagcc aaaagaagat
    10621 aaatatttag attatgatca atttaaatct ttaatgaaac tcgtagaaga aaacctttct
    10681 gcgcagtact cttctcctat gctcgtgtta gttgctggtg ccactggaat gcgatttgct
    10741 gaacttctag gattaacatg ggaagatatc gatttcgaag atcaaatcat cactattaat
    10801 aaaacatgga attataaatt aaatgaatgg ggaaaaacaa aaaacgaaac ttcaaatagg
    10861 aaaatttcca ttgataaaca tacgattgat ctcttaaaaa agtttaaaat caatcaaaaa
    10921 gaattattcg agaattttga aatcaaaaac cctcataatt ttgttttttt caacttaaaa
    10981 aatggattag tttcatcaaa cgccgtcagc aaatatttgc gcaaaaaatt aaaagaatta
    11041 gggattgaaa agcaatttac tttgcatgga ctaaggcata cacatgcatc tattttactt
    11101 tatcaaggag taaatatact tagcgtatcg aaacgtttag gacatagcag tttagaaact
    11161 acaatgtcta cttatcttca tattgttcga gagcttgaag atcaggataa agaaaaaatc
    11221 aatactgtgt tcgatagttt atataaaaat gataactagt ttggcacatt tttggcacaa
    11281 atacaaaaca aacccctgta ccgcaaagga tacaggggtt atttatacgt tcctgactgg
    11341 aattgaacca gcgacctatc gcttaggagg cgattgctct atcctactga gctacaggaa
    11401 caatttcaat aaatcaattg tagcttgttt gttttttact tgcaagctta tctgctaaaa
    11461 aaatcagctt ataagttagg caaaacgttt ccactagtcc ttaacagatg ataaactaga
    11521 ggtagaatga aataatgtag ggaggtcctt tccaatgaat gataagccaa aagaaaaagt
    11581 atacgcagaa cgcttagatt catctaaaaa aaatcgggaa aagattacga aagaagcaat
    11641 ccaagaccac aaaaaagaaa accctaaaac aaaagaaaac aaaaaattca aatttacgaa
    11701 aaaagaggat ttactttaac attttccttc aaatgccaaa tgaaaaacaa aagggtggtg
    11761 acaaaaatat tgtcatcacc cgttttttat caaaaatagt cattaagaat tggctgttgc
    11821 tgtggcaaga ttgagcttaa cgtttcttca tattcttttg tcacctgtat tctttcttgg
    11881 aataccaact cttccacttt acgataccct aataatagct gcgtgatatg ttggatactt
    11941 cctcgaacga ttgggagttt tgtttgttct acttttgaga gatgattttt tccattttcg
    12001 attgttactt ccacgattcc ttcattccat ttcgcataat gatcttcttc tatttgtagg
    12061 gcaaatgcag cttgtctctt tttaaatgga taattttcca aaaacaaaga aatatctacg
    12121 atcctcgcca tcatataagg tgtgatcatt acagaagcaa atggattttc taatagataa
    12181 ttttgatcat taccgccgta gccgcatgaa tattcaaatg tttcaaaagc accattgtga
    12241 gaacttacga agcgaacaag gctgcaaaaa gcatcatgat ccaaaaatcc ccactccgca
    12301 ataataaagg aagacgttgc aagctgataa acgatatatc ccgttgcttc ctcattatca
    12361 tcaaaatgca aagcaaactg gtaattgcct ttttgcttga atttatacgt ataccaccag
    12421 tcttcgcgct tcagaccagc tcgttggttt tccggcatct gttcataaat tcttctgatt
    12481 gcagcttttg catcttcaaa atccatacgc tttatttttc cagatttttt ttgataaacg
    12541 gcacatcacg actgtttagt tgataactga ttttatcaaa tgattgttca tagccatagc
    12601 gccgatagaa gggataggag aatggcgcca gataagataa gcttacttta cgatcacggc
    12661 aatcttcaag gatcttttcc atgatctgat tgatcccgcc ttgtcccctt gcttctggat
    12721 aagaagcaac atagccaatg ccggccatta ggtactcttg tccgtaaaaa ttcactggaa
    12781 aaggggtcgc catgacttga cttgttagcc gatcctcctc atcaaaaaat ccgtaattcc
    12841 aagaattctc cgcaagtgta cgaaaacgtt ctcgcctttc aggtgtatct gccccattaa
    12901 atgcataagt ggctaaatca aacatttcct caatcgaaga tacacctagc tctcttactt
    12961 tgtttgtcat tttgctgccc cttcctacaa aaaagctagt gaggattcgc aggaaagatc
    13021 tatcttccta tgaaaaattc cttctagctt acttcatttt tcaataacta aatagtacca
    13081 tgtttcattg atttaatacg ttgcttcgat catatctagc atttttttga aacgtctccg
    13141 tttaaaatac atcaagattg ttcctaaaag catatcattc aacttttgaa gcatgccata
    13201 agactccatt ttttctgtgt aatgaacctc acaggtcgaa tcgtccaatg gtttgatttg
    13261 ataatgaacg acgaattcat tcttcgttgt cgatgtacga aacttataag actggttttc
    13321 aatgacttct tcgattttta tgcgagcccg gttattttgc gagaattgtt tgacgtactc
    13381 caaatttttc agctgttttc tagttactga tttgccggtc gctttacgta catcgaaaag
    13441 gacagagtcc ataactttat cgtaaaaaaa cgatgctgga atatttaatt ttttcgtgat
    13501 ttccattagt tatttttctc ctttgcttct tctctttttt tccccttcat gaagaaatag
    13561 attcctaatc ctgttagaaa aagacctgtg accatactat acaaatcata cctttgcgaa
    13621 tgtggattca aactatacaa cacgatcaaa gaaccaatac tacataaaaa taaaccgttt
    13681 tttatcatct caaccatcct atctttttaa accattttct aaacagctgt tgattgaaaa
    13741 taaaggactg ttccccaagg ctgtgctttg attagttcct ttgctttttt agccgcaact
    13801 tccggatctt ctgcacttgt ttcaaagaac agcttgattc cttgtttttc tttaaatgta
    13861 aaagtcattc cttcttcatt gtatccttct aacaattctt tgatttcatc ttgttgcatt
    13921 gcactggcaa ttgtaatcat ttcagttcct cctcgaaaat tcattatgag gaacgaaact
    13981 taaagcgtcc cgctcctcac gttcatctta ttgttgttcg cctgcggcag ggtccatttt
    14041 gtttgcagag atgacaaatg gtgcccaaat caagaaagtc acgaccatac atactaatgt
    14101 tacgatcggt gcccgccaat ccgctcctgt tgctaagaat gacaataaga acggcggtac
    14161 tacccatgtg acttgctgcg ataccggagc gactaggcca agataagtag ctgtccatcc
    14221 aattgctgtt gctactactg gagcaatcaa gaatgggata aacatgattg cattcaatac
    14281 gattggcaga ccaaacatca ctggttcgtt gatattgaag ataccaggcc caatggataa
    14341 tttagctact gttagataat ctgcacgttt agagaaaatc aagatagcta tcagtaagac
    14401 gatcgttccg cctgatccgc caaaccatgc aaaagcatcg aacgatccgc ggacccacat
    14461 ataagctttt ccatcttcga ttgcatctaa cacggctggt gtaccagttt ttccgttata
    14521 acctttttgg aaaatatcaa tattgattag ttgtgcttgt ccccagattc cctctagcac
    14581 tggtgccaat acatttggtc catgaatacc aaagaaccag aatacttgaa caaatactgt
    14641 gacgatcaat actgctccca acccttgaga taatcctagg aatggttctg caatatattt
    14701 ttgcaccaaa tcaatgatca gttgcccatc agctacacga cctacgatat aattgataat
    14761 agcaatgacg tataaagcga tcgtcgctgg taaaatggct gcaaatgctt tagatactgc
    14821 tggtggtact gaatcaggca ttttgatcgt aatgttggca agcatcaatt tacaaaaaat
    14881 aatgactgaa atagctccca tgatcataac tgtaaaataa gcgtttccat tcaaatgatc
    14941 tagttttagc caaccccata atccaactga cagtgtgcca tctgcaatcg ttgcaccaat
    15001 ctcagaacta ttgattgcat ctgcgacatt tgaaggaaca ttcacatcta atgctgctga
    15061 taatgctgga ccaaaggaaa taccttgaat caaggtagct aaaccaacga tccccccagc
    15121 tagatcattt actccataag ctcgggcaag attatacccc caagaaaagg caaagatcaa
    15181 tccggcgatt gctagtgtac cgttccagac aaaaccattg attccgatga tgacattcaa
    15241 tacggcaaag attcctttgt ctgctgctaa actgtcagca taggtactgt taaactgttg
    15301 cggtaaatca cgaataattg cattgatcat tacagcgact gatcccgcca ttgttgcggg
    15361 cagtgtgccg ataaaagcat cacgcaaagc aactaaatgt ttttgcgctc cgatttttgc
    15421 ggcaaccggc aggatgtatt tctccatcca acttgatagt ccgttcattt tttacttcct
    15481 cctataagtt ttttatataa agagagttct ccttataaca aaataaaaaa tccgttcaaa
    15541 aatttttctt cttccgttca aaaaagaagt tgtatttatt cgctgtatac gcgacgataa
    15601 agttcgatga tttcttttgc taaatcagta aacgcaatag atgtcattaa gtgatcttga
    15661 ctgtgaacag tcaataatgt cacttgcata tgatttcctt gtgcttcttg tgtcaacatg
    15721 tctgtttgtg catgatgcgc ttgtataagt gattcttctg catcagcgat tttcctatcc
    15781 gctaactcaa aatcaccgat tttagcagcc gcaatcgctt ccattgcatc gctctttgca
    15841 ttaccgccaa acatgattaa ccccatgatg gcttctagat tttgttgatc ttccacgttg
    15901 ttataccccc aactgcatac ttgatttatt tcattagatt ttctgcttgt gcaagaactt
    15961 tttctccgtt catcatgcca tagtcttgca tattgatgac atctaatgga atacctttag
    16021 gcgcaagttt ttgttcaaat tgagctttca tgaaacgtac ttgaggacct aataataata
    16081 catcgacatt cttagattct aaattattat ctgcatctga ggcagagacc gcaaaaatat
    16141 cggcttccat ccctttttct tctgctgctt tttgcatttt tgtgaccaat aaactcgtac
    16201 tcatccctgc tgaacaaact aacatgattg ttttttttgc catttttaac actcctttta
    16261 tttttttaca cttttatata acgcaagaag catgccaata aaaaaaagag ctgaaatagc
    16321 gttttcatgc tttttagctc ttttttatag tgcgcacaca gatgtttagt gtgtaaacag
    16381 tatgattagt gtgttcattt ttttatttta ttttcaagaa acatttgtgt aagaaatgcg
    16441 atatcatctt ctggaatttt tacctgatat tttcgttcta gtgccatcaa gtttgcccga
    16501 acgatatcca tttccaaacg ataaattttt gagaaaatag acagtttctc aaattgccga
    16561 tggctttctt gtttgatcaa tccgtctaac atgaaggcta aatggataac gattccagta
    16621 tctactccag gttccacgat aatcgacaaa tcggtctgga tctgctgaac tgtcttttgc
    16681 aatgaacgga tcagttcatc aatagaaaag acatcgctta atgatccagc aagtgaatcg
    16741 acaattttag ctgtcgggat ctcatcatca gctatacgct ttaaaatatt tagtttttca
    16801 tcatcaaaaa cgtcataggc tgaaaaaaat gggatatttt gataatcaac atcaactgtt
    16861 cccgcaattg ctttgatctc atattcttcc aataaggcat cgatgtgttt tttgaatgat
    16921 tcacgttcta aaaactgcat ctggatcagt tctaccttat tttgattaat cactggcata
    16981 atacgctgat ataatttggc agctactcct tcaccagtaa agcatgtcac gattacggct
    17041 tttttacgct gtaaagtttc agacttttcc gctttgaact gatcacgtac gatcgattca
    17101 aaggaaagct ggatattttg ataaatatct tctaagcttc ttccaacact tgccatccgt
    17161 atcgcttcta tcacaatcat ggtacttgtc atcgtgatag ctttcgtccg aatgccagtc
    17221 tcttcataaa tcaaatcact gaaggtgttt aatgatccca tatctgttaa caagagtagg
    17281 ccattcgtca aatgctcttg atagtttttg acatattgta acacttcttc atacattgtt
    17341 tgaacttcca taaccagtgg catattaaaa gctttcccta cttttgttcc taatagttct
    17401 tgtgccatct ccaacatgct tgaagcagtc tgtctgccgt gcatcaatat gagaacatct
    17461 actttgtttt ctttaacagg ctcactttct cctagattga tagaaagaaa catactaata
    17521 aagccgattt catcaaaagg aatctcaact tgttcttctt cttcaatgat cgttgaaaga
    17581 tcgattgcta cctggaattc cctcttaaaa tttttccgta cgttattcaa atctgggtgg
    17641 acgatcatat gaccttctct cacacgatcc aatgtacttt gcagatgcaa agcaaaagca
    17701 aaacgagctt tttcattata aatacggtct aaccgttttt cagccacatc atacaattga
    17761 ttggttaacc gccagatttc atctggcaaa agatctttgg gaatcgaact tttagtcaat
    17821 tctttgacat atgtctggaa ataagcatcc acatcttttg aaatgagggt ctctagatca
    17881 atgttctcca caccggtttc ggaaagagaa gtgacttttt cctcaatatc gttatagacc
    17941 tgcatgtttc gttctggatc ttgtgaccag acaacatcag caaatcctgg ttcaaatgtc
    18001 aaatactgac ttttgctgtc catgaatcgg tcgagacgtt ccggtatttc cttgattttc
    18061 agtaatcctt tttgtacttg aagggaaagt tcttcttttc gaatgattag ctttttctca
    18121 tgatgagtac ggtaatgtaa aaaagatttt gcacagacta gtttcaagtc tcgtttcact
    18181 tgaccgatat ttccttccgc atcataaagc atgaaggcaa gaatcgcttc tttttcgata
    18241 tcgatacgtt gattcaaacg attcgcttct tgtttgataa aaagagagat gatttcataa
    18301 cgctcatcca gtgaacgtga agcaagattc ggcaaggtga ttgccatagg aatccgtcga
    18361 ttgaacgtag ataagaaact ttctgaagat tcagtcgtcg ccccaatgat ctgaacagat
    18421 gcttcatgta cttgtgcact ttctccaagc ggacggtaaa tacctttatc aatgaaagta
    18481 aacagcattt cctgcccttc cggcggtaaa cggtggatct catctaaaaa caatataccg
    18541 ccgtctgctt tggctattag accgggacta tcttgagctg ctcctgtgta agcccctttt
    18601 ttgatcccaa agatgtgtcc aaagagcagt tgcggatttt gagcataatc cgcacaattg
    18661 aaagaaacaa atggcgcatc tgcagaaagc atctctgaat cgattgcaaa atgatacata
    18721 cattcagcaa acatcgactt ccctgtacca gtttctccaa aaataatcgt atgcaacccc
    18781 cgcggtggat aaagaatcgc agcttttgcc tgctgtatgc tgacttttaa agaatcattt
    18841 tctccaacga gtgtttcaaa agtcacttgc gtagaagagt cgacatgcac ttcatcagat
    18901 tttgactttt ccgttggcaa agagtaaacg actggtcggc cagcggtttt tacaatatga
    18961 tgggccttgt atagttcact taaatatcgg cttgcattgc ttcgatcgat agttaagcgc
    19021 tcagctacat cacttgctgt caatccttgt tggttttctg tcaatagctg caagatctct
    19081 tcttgacgtt ctttcacata acccctcctc tggcaaccaa ttttgttccc gttttcataa
    19141 tcatagcata aaaaggcgct tatttgcgcc ttgctccttt atttctctct ttttttgatt
    19201 tatttttaac tttttttctt attttttctt tgttatattc tgggttctgc tcctttccag
    19261 aaaaagtttt cgcatacttt ttatttactg caggagctgc atcttctttg gttggctgct
    19321 cagtttgcag cttacctccg tataaaaaaa tctcttcaag tgttatattt gatggacgta
    19381 aaagccgttt taaatctctt agattattat cttgtacgag tgtaattact attccttcat
    19441 tacccattcg accagttctt cccgaacgat gcaaataagc ttcttcactt aacggaactt
    19501 ctgcattcac tacataagga agacctgtaa tatctaaacc tctagccgcc acatctgtgg
    19561 tcaaaagtgc tctgaccatt ttctttttga attgttctag cgctgctttt cggagtagct
    19621 tgttctgatc agaggccaga cttcctaccg gtacacctct atacagcaat ttttcttcca
    19681 ttacgcctaa ttcagtcagt tgattaaaga aaatcagacc atgaaaatct tctatatgcg
    19741 tgagacgtct caagtagtca tctttctttc gtatcggtac acgtaaaaag taatgtctca
    19801 gtccttttcg agaatcgtcc tctgctgtca cgtcaattgt ctcaaacgtt ttcccagtga
    19861 ttttttcaat gctttcaagg gaacgatctg ctgtcgctga aaaaaaagct agctgatact
    19921 ctgttggagc ttgatgaaga atttgatcaa tgatttggct gttcccagcg tcaaatagct
    19981 ggtctgcttc atcaaatacc attgttttca cttgatgtgc ttttattttt tttgctttca
    20041 tcaattccaa tattcttcct ggtgtaccaa tcaagatttc tggtttttcc ttcaaaccct
    20101 caatctgacg tttgacgttg gctccgccca ccaaagattg gactttcaac tctttgtctt
    20161 ttccccattc acgtgcaaca tctgctactt gaatagctag ttcttgagaa gaagcaaaaa
    20221 tgaccaacgc acttgcttca cctggttcca cgtttagtag tagaggccaa agataagcca
    20281 gtgttttacc tgaacctgta ggagaaaccg ctactatact ttgtttatta cttagtggct
    20341 gaaaaactgc tcgttgaata agagaaggtg agataaaccc tttatcctca tattttttct
    20401 gccaattttc gggaaatact tgtacaaatt cgttcattaa aaatcttcct cattatctgc
    20461 atcgaatttg atgccgccat tttttcttaa atcataaatc acttggttca catttctagc
    20521 tagctcgatc cactcttcat acaacaatcc ccaagctgga tctttcggat gagccatgac
    20581 attggcaaaa tcctctgctt catctgccat tggattttct gcagctgtta ccgccaaatc
    20641 aatgatttct ttatgcgcgc gatcatgaaa ttgtgcttgt tcaattgcat tgactccatt
    20701 catcactagc gttcctgagt caaaataaat ctctgacggt aaataagaat caccgatttt
    20761 acctggctgg atcgatacat caaaattctc ataacgtaag atcccccatc caagaccatc
    20821 tacaccagta gaaatctttc ttgggaaata atggacttct tttggcattc caaaccaagc
    20881 aacagctgca tatacaaggt aaacacctaa atccatcaat gcaccacctg agaaatgagt
    20941 cgagaagata ttcggttctt ctccatctaa tacttggtca tagcgcgaag aatatttcat
    21001 atagctgaaa tttgcaccga ttatctcgtc ttttaatggt aagtaatcgg caatcttttt
    21061 aaaacctgat tcatggatat ttcttgctgc ttcaaaaaaa tacacattgt gttcatttgc
    21121 caaatggatg atttcatcca tttcttttgg cgtcgaaaaa gctggctttt caactatcac
    21181 attttttcct gccaaaattg cttgcttagc ttgactaaag tgaagactat tcggagaagc
    21241 aatataaatg gtgtccatat gagtaatatt gaaaaaagtc ttcaaatctg ttgcgaattc
    21301 gatatcccct tcataatgag aaccaaattc ctgtgctttt tccaaatgtc tagaatatac
    21361 cgctgtcaat tcatattttt ttgtgcttaa agctgcttgg acaaattgat gagtaatcca
    21421 atttgttccg ataataccta agttgatcat tattttacct tctttctatt gtataggatc
    21481 ttcaaaaggg atatctacat caaatggaat ggcctgcttg acagttacct catctgccga
    21541 tacttgacaa ttataggcga ggatttgaac tccgctttgc tgcgcatgtc gaaccatatc
    21601 agctagtgct ggttgcattt cttcatggat cgttgccaga tgaagatgtt cgaactgagc
    21661 aataaacaag acataagcgg cataaccatc ttgtgcggca tacgttaatt ctcctatatg
    21721 tttcaatcct ctgagtgtgg gcgcatcagg aaaagcacca atcgctttat tttcaagtgt
    21781 catacctttg acttcaacaa atgctttttg tccgaccacc gtctctaaat aaatatcaaa
    21841 tttggagtga agataagtta cttcacgctt gtataccgta aatggaccag ttaatcctgg
    21901 taaaaggatc gtgccgtcta aaataccatc aattgccagc cgattaggca attgactatc
    21961 gatattaaac cagtcaccct tcttttttac agcaatcaga tcataggcgg ttttccgttt
    22021 aggtccagga gcaaatacaa tagccacttc agcgcctggt atcaggacct cttttcctct
    22081 acctgtattt ttgacatgcg caataattat ttctccgtct tttttcatac aatgtgctat
    22141 aaaccgattc gggcggtcaa taaaaaaagc tatttccact tgctgatact tcaaattatg
    22201 tcacctttct tcctactatg tatcctatca taatttgtag aaaaacagta aaaaaaccag
    22261 tcgaaagtct tagagaaatt ttagctttta ttcggtacaa tgaaataaag aagaaagaat
    22321 atgtctatgc gattattttc tcataaagga gtgatttggt gataaaaaaa atgatctatt
    22381 ttcttggttc actcattacc gttctctcaa gttttattta cttgaaaaag aaaaaacaag
    22441 cggaaaagtc agaaggactc tcaaagtatc aaggtacatg gttctttagt gatagccagc
    22501 aaaaacaaca attacagatt acatctgatt acacgttttt gatcaacggt cagtctcttg
    22561 gcacatctgt ggtagaaata gatgaatatc gtttagtcgt acgtgatcag tatggttttt
    22621 atcttatctt ttcttatccc gaacaaactc tttatgacga atctgcagac acttcatata
    22681 agttgttatc tgaaaaatag atccctccca atgtatatat atatccttta taaacgaaag
    22741 aatgagtctt ctctatcatc tttcgttttt tgttgtaaaa acgtctaaaa atcctttgtt
    22801 ttttatttag aaaaatagtc aattaacgtt ttttttgtta cacttactaa tgatggtttt
    22861 ttaccatgaa aggatgatcg aatatgacac caaatcgaga agattatttg aaaatcattt
    22921 tagaactagg tggagataca acgaaagtca acaataaaca aatcgtatct agtctagatg
    22981 tctcagctgc atctgtcagt gaaatgattt ctaaacttgt gaaagaagag cttgtagaac
    23041 attcccctta tcaaggtgta caattgactg caagcggact gcaaaaagct agcagtctga
    23101 ttcgcaaaca tcgcttatgg gaagtttttt tagttgaaca tttagactat agctggaatg
    23161 aagtccatga tgatgctgaa gtattggaac atgtgacttc ggaacatctt gcagatcatt
    23221 tagaaaacta tttgaatcat cctgaacatt gtcctcatgg aggatcgatt cctaaaaaag
    23281 aggaagttgt ccatgaagaa cgccgacaaa cattggagtc ctatcctgtc ggtacaaaag
    23341 tgcgtatcgc acgagtgctt gatgaaaaag aattgttgga ttacttagtc actattgacc
    23401 tgaatatcaa cgaagaatat gagatcgttg acatcgcagc ttatgaagga ccgatcacta
    23461 tccaaaatga tagaaagaaa atccccgtca gctttaaggc ggccagcacg atttttgtcg
    23521 ataaaatata aaggagtgta tccacatgtc taatgaaaaa aatgtattgt ttacgatatt
    23581 cggcggtaca ggtgacctag cccagcgtaa attgtacccg tctttgttcc gcctttacag
    23641 aaaaggaaat ttaggcgagc attttgctgt aatcggaacc gctcgccgtc cctggagcga
    23701 tgagcactac cgtgaagttg tcaaagaaac aatccgtgca ttgaatcctt cagatgaaga
    23761 agcaactacc tttgcaagcc atttttacta tcaatcccat gatgtaaatg attcaacgca
    23821 ttatcagaca ttaaaagaat tgtctgaccg attagatgga cactatcaat taaacggtaa
    23881 ccgtgtctat tatttggcta tggcacctca attttttgga acgatcgttt ctcatttgaa
    23941 atcacaaaat attatgacaa aaaatggctt caatcgtttg atcatagaaa aaccgttcgg
    24001 ttctgattac cagtctgctt ttgaattgaa ccagcaaatc agagaagtat tcccagaaca
    24061 agatattttc aggattgatc attatttagg aaaagaaatg atccaaaaca tctcagctat
    24121 ccgttttgcc aataatattt ttgaggctca atggaacaat cgctatatcg acaatatcca
    24181 aattactttt ggcgaaaacc tcggtgtaga agatcgcggg ggctactacg atcatagtgg
    24241 cgcacttaaa gacatggtgc aaaatcatat tttacaggtt gtagcattac ttgcaatgga
    24301 accgccagta gcattttccg aaaaagaaat ccgtacagaa aagatcaaag cattgaaagc
    24361 tatccgcatt tataacgaag aagaagtgtt agaaaacttc gttcgcgggc aatatgctca
    24421 aggtgaattg gatggtgtac aattcaaggg ctatcgcgaa gaagacaaag tggatgctca
    24481 atcagagact gaaacattcg tcgcaggcaa attcactatc gataacttcc gttggtcagg
    24541 tgttcctttc tatgtacgta caggcaaacg tttgactgaa aaagggactc ggatcaacat
    24601 cgtattcaaa caagttcctg ttaatgtatt caaaacatct gtcgatgaac catgtgacga
    24661 tacgacactt ccacctaatg ttctaacgat ctatattcag ccaactgaag gattttcttt
    24721 atccttgaat gggaaagaag tagggcaagg atttgaaacg gaaccaatca aattggaatt
    24781 tagaaatagt gcagaaatgg ttgaaaatag cccagaagct tacgaaaaat tactattaga
    24841 tgcgttgaat ggtgatggaa cgaacttctc tcattgggaa gaagtcgctc aatcttggca
    24901 tatcgtggat gtcattcgaa aagcttggga ccaaacacaa ccggattttc ccaactacaa
    24961 agctggatca atgggaccta aagcagcttt cgaactactt gaaaaagacg gattcgaatg
    25021 gatctggcaa ccagataact ggtatcgtga acgtggaaaa ttaaacgatt gataacaaaa
    25081 gggggtgtga caaagaacat gtcacacccc cttttccctt ttacttttct tcgaatcctt
    25141 cagctacagc ttcaggatag aagtcttcaa cgatttttgc acaacgaccg catagatgag
    25201 gaagtttttc atctactcct acatctttac gaacttggcg acagcgatcg caagtttccc
    25261 cgtctgcttt ttccactgta atagcaacat catcaaatac catagcgtta tctggcactt
    25321 gttcatctgc tgccataact tcaaagtagt ctggtgaaac gatcaataat tgtgcaatat
    25381 ctgcatccaa agcttttaac atttcacgaa cttgttgttt tggataaaca gttagttttg
    25441 cttccaaaga tttaccaatc aatttgctgt ttcttgcttc ttccaatgct tttaagacat
    25501 tatcacggaa gtccatgaaa gcagtccagg tatccatcaa ttcttcttgg tttgcaaact
    25561 cttcataacc aggaaactcg cttaactgaa cgtactcctc ttcttctttc aggaatgacc
    25621 agatttcttc tgctgtatgc ggaatgatag gagtcaataa ttttgtcaaa gctacagctg
    25681 tttgatagaa aacagtttgc atgcaacgac gttgataatc atcttctgct tcaatataaa
    25741 ctacatcttt agcaaaatcc aggtagaatg acgatagatc aaccgtcaag aagttcataa
    25801 ctgtacggta aatatgcatg aagttatatt tgtcgtaccc ttcttctcgg atttctttga
    25861 ttacttggtt caatcgaacg gtcatatatt tgtctacaga acgtaaatca ttgtacgaaa
    25921 cagtatcttt tttaggatcg aaatcttctg tattcgctaa taagaaacgc atcgtatttc
    25981 tgattttacg atatacttct gccacttgac ttaagatatc catagaaaca cgaacatctg
    26041 cttcataatc tacactagat acccataagc gaaggatatc tgcaccaaat tgtttgatca
    26101 ctttttctgg tgcaatgatg tttcctaaag atttactcat cttacggcct tcaccatcta
    26161 aagcaaatcc ttgagaaata actgatttgt atggtgctac accattgata gccacactag
    26221 tcgtgatact tgagttgaac cagccacgat attggtctga tccttctaaa tacatatctg
    26281 ctgggaatga taattctggt cgctgacgta aaacagcttc atgagaagaa ccagaatcaa
    26341 accagacatc catgatatct ttttcttttg taaattcgcc attaggagaa cctgggtggg
    26401 taaatccttc aggaagaagt tctttcgctt ctttttcaaa ccagataatt gatccatgtt
    26461 cagcaaataa ttttgccaca tgttcgattg tttctggtgt gatgattgct tctccatttt
    26521 cagcatagaa aatcggaagt ggtactcccc atgcacgctg acgggaaatt acccaatcgc
    26581 cgcggtcacg gatcatatta tacaaacgtg ttttacccca tgggataacc cactctactt
    26641 tttctacttc atccaaaata ttctgacgga atttatcgat agatgcaaac cattgtggag
    26701 ttgcccgata gatcacaggc tttttcgtcc gccaatcgtg aggataacta tgagtaaaga
    26761 aatctagctt cagcaaagct cctttttctt ttaatagatc agtgatcata ggatttgctt
    26821 tgtcataaaa gataccttca aatccaggtg cttcttctgt aaacaatcct tttgagtcta
    26881 caggtgacac tacagatagt ttatatttgc taccagcgat atagtcatct tccccatgtc
    26941 ctggagcagt atggactaat ccagtaccag cgtctaaagt aacatgatcg cctaaaatca
    27001 caagtgattc acgttcatag aatggatgac ttgcagtcat atattccatt tcttgtccag
    27061 caaattcttt tagaactgtt acattttccc aaccgatttc tgattggact gtttctagca
    27121 aatctttcgc tacgacaaac ttacggccat cggcttccac caatacatac gtaaattttg
    27181 gatttacagc aataccttgg tttgctggca atgtccatgg tgttgtcgtc caaatgacaa
    27241 aagctgtatc ttcatcaaga atatctttgc cgtctttgac attgaaagct acaaaaatag
    27301 atggagattt tacatcttta tattcaattt cagcttccgc taaagaagac tcacttgacg
    27361 gagaccagta aattggtttc aatcctttgt aaatatagct tttttcagcc attttaccaa
    27421 atacgcgtac ttgtgcagct tcataagaag gatctaatgt aacataagga tgttcccaat
    27481 ctcctgcaac tcctaggcgt ttgaaatctg ctcgttgctt atctacttgg gaaagtgcat
    27541 attcacgaca tttttccaag tattctgcca tggacatttc tttacgtttc acgcctttat
    27601 tagccaatac ttgttcaatc ggcaatccat gtgtatccca tcctggaaca tatggtgcac
    27661 gatagccaga cattgatttt gaacgaatga taatgtcttt actgatttta ttcaatgaat
    27721 gtcctaaatg gatattacca ttggcatatg gaggtccatc atgaagaaca aagctaggtt
    27781 ttccttcgtt caactcttgt cttttttggt agattcgatt ttcttcccat tctttttgcc
    27841 actcgccttc tcggtttggc aaattaccac gcatagggaa ttttgttttt cctaattgca
    27901 aggtttcttt cattttcatt caaccataca ctcctttatt tgttttccgg acacaaaaaa
    27961 gcatgttcag ccgcaaggga cgaacatgcc gtggtaccac ccaaatttag ttgcatctat
    28021 ctagtgcaaa ctctctgtga ccaataacga ggtcagccgc tgcttcttac tctaatttcg
    28081 gaaaagcagg tctcaagtag aggatctttc accatgcagt gtcttaccga acttccactc
    28141 tcatcggctc gctttaagaa taacaaagtg cttttctgtt ctactcgttt tcattttcac
    28201 caggcacgat cgagatatca atagcctgtg tatcaaatgc cgtcataggt gcttctatga
    28261 ctcctgtgtc tgagtttaca tcagtatcat tttcattgtc aagctgtttt gctaaaactt
    28321 ctttgatcac ttcatggctg tcagtcacgt agctggagaa tggttgtaaa atttcatccc
    28381 attccggact tttcacttgt tctaattgag actcaagcat caagctgata cgttgatgga
    28441 atactcgtgt ttttttctta agatcttcag tttctgtcgc aagctgtctt gctttttctg
    28501 ttgcatctgt caagattttt cttgcttttt cttctgcatc agttgttaat tgatgtgcac
    28561 gtttttcagc gttcgcaact aactcatctg ctttgttttg tgcagaagta acgatcactt
    28621 cggattcctt gcttgcactt gtttttactt tatcagccgt atcttgagct acgatgattg
    28681 attggttcaa agcatctttt aattcattga agtactcaag tttttcttca gaatgtttga
    28741 ctgctttttc taattctcga tttctttgag tcaattcctc atagtcacga acaactaaat
    28801 ctaagaaatc atctacttcg tcttgattat atccccgcat ttttgtcggg aatgtcttat
    28861 tttggatatc taatggagtt aaagccatga gtaaaccacc tttttatttt atttacgtaa
    28921 tacaccgaac agtaccctgt acttttcttt tttcgtcttc ccctcaagtt cttggatctg
    28981 gactctgcca taacctctga ctgaaataat atctaatagt tcaagtacaa aatcaggtct
    29041 tgtattttct gtccagttca cttttacttt accagcttcg atcagctgct tggaccgctg
    29101 tctggaaata ttataaatct cagagatcac actgtctagt cgcatggaac taactgttcc
    29161 tctctcttgt gtccagctat ctttcggcat aatcatttct gtgtatttga ttggttccaa
    29221 gcgtacagca acctttccaa tctttgttac ttgtgcagca acaaaatgat ccacctcatt
    29281 tgcaataaat acttgccagc gatcaccgtc cgagatgata tcgccaaagt aagagcgttt
    29341 gatccccgtt cccactaaag ttccaaggat tttcccatga cttaggctgg caaatttgct
    29401 aggataatgg atttcataca atgctgtttc aaaatcttcc tctacaggtt cgtaataatc
    29461 ggggaaaatc aaacatcttt ttctttctgc ctgttcatat ccgccataaa acatgaaacg
    29521 caaatcactg ctttgtcgaa tcagtgtttc taaaatatag gattgtcttg ggtcaagaaa
    29581 ttcggtcaga tagggtgcat attgtccttg aacctgttca atccaatcgc ctaccgaatc
    29641 gataaatgta cgttcatctg cccgaaagtg ctggtataca tttgcatcca aaattttccc
    29701 ctcctatcta ttctgagaat aaagattttg ttgcttcaat caataaacta aatggacagt
    29761 tatccatata acgatctgtg ctaaaccttg tacggctaag ttcaagatca ggattgcccc
    29821 taaaattgtg aaatccaagg gtccaatcct caaaggcaga cgatcgaaca aactgatata
    29881 cggttcgcag atcttagcta ataatcgtcc tagccctgat tgatacccgc ctggaaacca
    29941 agagagcaat gcatatacca tcaagataat gctatagaaa taggctgccc gttgaagtaa
    30001 atgaagtaat agtgcaatag tataaatcgt agttcctcct cttacagatc aaacaaattc
    30061 gcatctgcaa aagattgagc ggttgaactg tcaatctcca tcccagtcgg tgtacaaaga
    30121 aaaatctcat ctccaacacg tttgatatct ccatctagtg cataaactgt tcctgttaga
    30181 aaatcaacga ttcttctcgc ttgtttttct tctactaaat gaaaattcac taaggttgct
    30241 tctccagcaa tgattcgttt agcaatcgcc atagcttcag aataagctct tggttccatt
    30301 acagtgattt ttccagcaag atttgcttta ggttgtgtag aggttcttga agctgttctt
    30361 tctttcgcta cagtttttgt aggtgattga tgtagttcaa ttactttttt cttttcagcc
    30421 ggctgaatac tttcatgcgt tacaggacgt gaagtcgccg ttttttgttt ttcatgagta
    30481 ttcgcagcag atacagaatg agttgattga ttctgtgttg tatttttttt agttggctgt
    30541 acagcctttt ttgttgtttc aggataatct tggtattcgt actcttcctc atcagctaat
    30601 ccgaaaaaat tagcaattgc tcctctgttt aaaagcggca tttttgttcc tcctttattc
    30661 gtctttgaat agcgctgttc ctacacggat aaatgttgca ccttcttcaa tcgcaatttg
    30721 aaagtcttga ctcatcccca tacttaattc actacatggt gcatgggcat aaccttttgc
    30781 ttgaacaaga tcacgcaatt ctttcagcgt accaaacact ttatggattt gctcatcaga
    30841 agcttcttta ggcgccatag tcatcaaccc aacaaccttg atttttgtgc aggcagctaa
    30901 agattccaca aacggtaaaa gctcatctgg tgaaattcca tgttttgatt cctcaccaga
    30961 aacatttact tcaataaaac aggataattc tttctctaac cgcttctgga tttcttctgc
    31021 taatcttaaa ctgtccaacg catgaaagta atcaatttca tttacaatca attttacctt
    31081 tctgcgctgt aaattaccaa ttaaatgcca ctttatttca ggaaaatcaa ataaatcttg
    31141 tttcttttct aataatttat ctactcggtt ttctgcacaa ttcataacgc cagctctaac
    31201 caattcttca gcttgagcgg attcaacaga ttttgttaca gctactaatg taacttcttt
    31261 cggatcacga tgaacaagag cacacgaatc ctgaatttct tgcttgattt tctgcaagtt
    31321 gtcagcaatc atgtgctctt cctctccttt attttttacg acggaaaaac ggtggcgtat
    31381 ttaattcatc atcgccatga gattttgttt cttcgcggtt gaatgtatca aattcttttt
    31441 tttcgatatt gtcaaaattg gaatcatcaa ctctaggacg aacattttgt tcacgtctga
    31501 tatcccaatc gccaaaactg ttttcttctt ctgaagaagt tggtttagct tgatccatat
    31561 ctaatacagt cttttgagca ggtgattgca tttgtgcttg tctagctggt cggctagact
    31621 tgcgttcctt ttttgattca tcgatacctg ttgcgatgac cgtcacacgg atttcatcgc
    31681 ccatttcttc attgatagat gtacctagaa tgatatttac atcgccagtt gctgcatttg
    31741 ctacgatatc tgaagcatct tgtgcttcga acaatgtcat atccaaaccg cctgtgatgt
    31801 taagaagtac ttgttcagct ccatcaatag atgtttcaag caatggagag gagattgctt
    31861 ttttcgttgc ttctactacg cgatcttctc cactagccac accgatcccc atcaatgctg
    31921 taccttggtt ttccatcact gttttcacgt cagcaaagtc aaggttaacg taacctggcg
    31981 ctgtgatcaa atcagagata ccttgtacac cttgtcgtaa aacattatct gcttcacgga
    32041 aagcttcaag catcggtgtc tttttatcta caacttctaa taaacggtta tttgagataa
    32101 ttaataatgt atccacattt tctttcaaac gagcgatacc ttctgcagca aaacggccac
    32161 gtttcggtcc ttcaaaagtg aatggacgtg tcacgacacc aacagtcaat gcgcccaatt
    32221 ctttcgcgat acctgcaacg attggtgcag cacctgtacc ggttcctcca cccattcctg
    32281 cagtgatgaa aatcatatct gcaccgtcta aagcttcgcg taaggattgt tcactttctt
    32341 ctgccgcttt ttgtccaact tctggctgag aaccagcacc taaaccacga gtatatttgg
    32401 ggcctaattg gataactgtt tcagcttttg aattttttaa tgcttgcacg tctgtgttgg
    32461 ctgtaatgaa ttcaacacct ttaacgtttt cttcaatcat tcggtttaca gcatttccac
    32521 cgccaccacc gactccgatg actttgatta ctgcgccgtc attgatatta ttatcaattg
    32581 agaattccat atcttattcc tcccaggatg aattaatcaa aaatatttgt aaagaaaccc
    32641 ttgattttat tttttacatt ttctccggac tcttttggtt ccggctcatc gtagattgtt
    32701 tcttcaggtg cttcatagct ctcatatgtg tttgttgttt cttgttcaac taccatttga
    32761 cgagctgctg aggtttcacc cgtaacagca atttttgcca attgatacac ttcactcaag
    32821 tttgctgaat aatccacgat acttattaca tttgtaaata ccgggtttct cagccccatg
    32881 tgatttggaa catacaattt tacatttacg ccgaagattt cttgtgccaa gtccacaaca
    32941 ccaggaagac tagcagctcc gcctgtcaat acgacgccac ctggcaactc taatgcttcg
    33001 atttggtcca atgcatcttt tgctttgttg aaaatctgtt ccattcgagc tgaaataatt
    33061 tctgacaagt agcgttcatc gactttcact ggttctgact gaccaatcac atctactgga
    33121 aattcttcat ctggtgatgt ccgttctggg tatgcatccc cgtagtttat tttcaacgct
    33181 tctgcattgt taaatgacgt atttaataca atagagatat ctttagtgac aaattcgcca
    33241 ccttcttggt caagattggt aaacttcaat tgcttatcat gcataacagc cgttgttgtt
    33301 tggccgccac ccatatcaat gacgatcgta ccaaaatctt tttctccgtc agaaagaatt
    33361 gattcagtca gcgctaaagg agtaatcacc atttcgttga cgattaatcc ggcattttcc
    33421 acgcatttac gtatgttatg gatgatcgtt ttaggtcctg taaacaacag accgtacatt
    33481 tctaatctta cgccgatcat tccacgagga tctttgattc cctcgaaccc atcgacagta
    33541 aaatcttgtg gcaatattga cacgatttga cgttcaggag gtattgaacg aacaagggca
    33601 gctgaagcca catttctgac atcttcatcg gtgatttcct ttgaatctcc gttaactgca
    33661 atcattccct ggcaattttc tacttcaagc atgtttgctg gcaaaccgac acttacgcct
    33721 ttgatctgga tacctgcttt ttcttctgct tgtcgtactg ctcgttgtat cgcttggacc
    33781 gttttatcga tatcaacgat aatgcctcgg ttaatccctt ctgattttgc gtttcctact
    33841 ccaataatat tcatttggct gtcgatatat tcagccacga caactttaac agatgtcgtt
    33901 ccaatatcaa ggcctacata cattcctgtt tttgccatga atgggattcc ctcctactta
    33961 ctattccata tacaagcgaa caactagcgt ccgttttaaa atatacttat taatagatat
    34021 attcactgct tccaattcta ccataaatta ctggttttta gaaagtacat tcttactttt
    34081 taaagttgtt ttatcaattt tcatttaaac ttgacggagt tgtacttgga ttatcttcta
    34141 tttgacttgt ttcacttgaa tcatttgaat cagatgatcc gtttgtgcta gacgatccag
    34201 gtgtttgtgc tttttgcgtt tctgcctctt cacttgattc gtttgtttga tccgcatttt
    34261 cttcactttc agttgtttct tccgattggc ccgattcttc cgtttcttga gtgctaccgt
    34321 aaggatgcga gaagatacca acttccatgt cgatcacacc tttttcatcc atatctttgg
    34381 ctacttgcga ataatatttc atttggttcg caagattatt gatatttaca ataacttgat
    34441 tgccgtcatt catattcaat tggatcagat ttttattgct tgctcttggc gtgtacttga
    34501 tttcagaaat tgctttttgt aattctgatg ataactgatt gtattgcttt gttaattctt
    34561 tgatttttgc agagtctttg aactcttcca agatagggag attttttgta ggatttgcta
    34621 ctttttcatc taatactgtt ccattttcaa tcactgggta gtattgattg ttttttgcga
    34681 tcaaagcaat ctctttatat tctgttacat ccaaatcgaa ttcattcatc cctttgaaat
    34741 gtatatttgc tgtttcgatt cttggctgtg cttttttaag gcgatcgata taagtagatc
    34801 gatgaaaata ctgtccccaa acattttctc ctaatgacaa ttttgtatca gagatgattt
    34861 cttggctgtt gactgtctta ttgcctgata ctgcaactgc ttgtaatttg ctataaggag
    34921 aaacataata gatcaaaaaa atcagcggta tacccaatac agtgatgatc aaagctaacc
    34981 gacgaaatag tactttgtgc cgttgacttt ttaaattggg cagccggttc aaaaaagaac
    35041 cgttatatgg tccaccgttt ttcttttgct tgatctcttc aaaaaattcg atttttttct
    35101 cggactggct ttcttcttgc tgttcctctt gaaccgtttt ttcttcttca gaatctgaaa
    35161 ggggtgtatc taatgttatg tcttcttttt ttggctcttg gtttttcatc tcttgttctg
    35221 ccagatattt tcggttagcc ttttgccaag gagtcaaatt gttttcatca gaagacggat
    35281 gagacaattt ttttggttct tttttcttac tcactaagac caccgccttt attttttgat
    35341 tatttgtttt actaagtcat aaagacgttt tgaagcatca gggattcctt gttctttaga
    35401 agctcggcac atttgctttt gcaattcttc atcacccatg atttcgttga ctgtctgact
    35461 tagtgattca cctgttagtt cattatctgc aatcattttt gctgctcctg cgtgaacaag
    35521 actcattgca tttttagtct gatgatcatt cgtaacatat gggctgggaa ccaacactgc
    35581 cggtaacccc agagctgtga attctgcgat cgaagttgca ccagcacgcc ccaccagcag
    35641 atcactggaa gccatgacct ctgccatatt tttgatataa ggctggacac taatattttg
    35701 tatttcagga tttattttat ttaggatctc ttcataatat ctatctcctg aagcatacaa
    35761 aacttggtaa gaagaaggaa tatattcact taaaaaaact gatactgctt gattgatttt
    35821 aagtgctcct tgactaccgc caaaaatcaa gactgttttc ttttctggat ccaaatcaaa
    35881 tgttttcaaa atatctgatt ttttagtatc tgctacttct tgtgctcgag gattacctac
    35941 aagaactgct ttgttttcag gaaaaaagga agccgcatct ttaaatgaaa gagcgatccg
    36001 atccacatat cggcttaaaa atttattggt aacacctggt acactgtttt gttcatggat
    36061 aactgttgga atacccattt tggctgcagc gtacactacc gctccggaaa catatcctcc
    36121 agtaccgata acgatatctg gttgaaaatc actgaggatt ttttttgctt gacggatact
    36181 cttaagaaac aatcggaccg tcttgatgtt atgcatggaa atttttcgtt taaatccttg
    36241 aatctccaag gtatggaaag gcattcctgt atcagggacg attttgtttt ctagacctcg
    36301 ttttgctccc acatacatga attcagcatt cggttcttga gtctttacat aattgacaaa
    36361 ggcaagagct ggataaatat gtccccctgt tcctccgcct gtaaccaaaa ttttcatttt
    36421 ttatcgctcg cttctacttt ttctatttta atcggttcac agcttccatg aatctgtttc
    36481 ctctagtttc aaaatttggg tattgatccc agctggcatt cgctggtgat aataaaacta
    36541 catcaccatc tgtactgttt tctaatgcta aaggaactgc ttgttctaca gaatcggctg
    36601 ttactattgt ctcgatgcct gctttttttc cagcatccat caacttttct tttgtttggc
    36661 caaatacgac cattgctttg attcctttta atgaaggaat taattcatca aatgtatttc
    36721 ctctgtctaa tccgccagcc aataacacga ccttagacgt ttcaaatcca cttagagcca
    36781 ttttagtcgc taaaatattg gttgcttttg aatcattata aaattttctg ccttggattt
    36841 ctcctacata ttgcgtacga tgtggtacgc catggaaaaa atgcaacgtt tctcgtatag
    36901 cttcatttga tataccatac aattttgcta cagaaatagc agctagtgca ttttctacat
    36961 tatgcttacc tggtacgcca agttcagata tttccattat tttttctttt ttatagtata
    37021 ttgagtaatc gtcagcatac acaccgtctt ccaacacttc ttttgtcgag aaaggcagta
    37081 cgcgtgcttt tgttctttgc gctaattctt gtagttctga ctgattccag ttaaggatca
    37141 gataatcttt ttctgtcatg tttttttgaa ggttccattt agctttgaca tattcttttc
    37201 ttgtaccatg ataatcaata tgtgcttcat agatattggt aatcaccgct atatgagggc
    37261 ggaaatctgt gattcccatg agttggaagc tagacaattc catcacaatt ttatcttctg
    37321 attttgcttc ttgcgctaca ccacttgctg gatacccaat attacccgct aaacgtgcaa
    37381 ttccttgatc tgctcctgca ttcagtagta gtcctgtcat cgttgttgtc gttgtttttc
    37441 cgttcgttcc tgtaatccca ataattgggc attccgctac ttcgtaagct agctcaactt
    37501 ccgtaattac cggaataccc atttcttggg cttttgctac taaaggatgc ctgtagggaa
    37561 tccctggatt tttgaccatc aatgaaaatt cttcatccaa caattcgatt ggatgactac
    37621 ctgttattac ttttatccct aaagaaagca gctcttgtgc ttccggattt tcgtcaaaag
    37681 gttttccatc attaacggtg acaagtgcgc ccaattcatg taataatttg gccgcactta
    37741 ctccgctttt tgcgagtcct aagacaagca ctttcttatt ttcatatgtt gatatttttt
    37801 tcatttcccg tcatctccta ccaaacgatc cataatgtga tcaacgaagt cacaatgctg
    37861 atcaaccaaa aaactgtatc aatcttccac tccgaccatc cgcacatttc aaagtgatga
    37921 tgaattggag acattttaaa aattcgtttt cctgtaagct taaaggaagt aacttgaagc
    37981 atcacacttg ctgtttccat aacatagatc aaaccaatca aaagcaatgt ccattcttgg
    38041 tttagcatta tagaaattgc cgctaaaagt ccgcccaacg ctaatgaacc gacatctccc
    38101 ataaaaatct tggcaggttt tctgttgtag acgaagaaac ccagtaaacc tcctaaaaca
    38161 cttaagcaaa tgattaatac atcatactga tgctgatgcc aagcaattag accgtaagct
    38221 gcaaaagaga tacttcctaa gccagcaact aatccatcga tgccatctgt cagatttact
    38281 gcatttgaaa atccaacaag ccagaagaga gcgaaaatac cataaatgat tcctaaaggt
    38341 agatctagtc cgaagaaatt caatacacct ggataacctt cagaacggta aacaagataa
    38401 aagatgattc cccctagaat ctgtccaatc aatttctgct tcgagttcag tcccatgttt
    38461 cttttcttga atatttttat aaaatcatca agaaagccga ttactccgta taacgctaat
    38521 acaaaaagca agatgaaaag agtaggagtt agctgttttt gccaagctcc aacccaaatt
    38581 cctgtcaaaa tagagccgat cagaaacact aagccgccca ttgtaggagt tccggctttg
    38641 ctattgtgcc actttggtcc ctcctcacga atcgcctgac cttgcttttt catttgaaaa
    38701 taaccaataa atagcggcat tgcggcaatt gtcattgcac aactgctgac aatgggaatc
    38761 aatgcttgtg tccattccat gctcttttct ctcctatttc tcttcttatt cttctaaagt
    38821 aaaactaagt ttgtcttcag tgattttttc atatggagca agttcttgtt tgacacaata
    38881 accatctcca tcaaaactaa cttcaatacc gaggattttc cccagcttca tgatatctgc
    38941 ttttgaccat cctgttacgt caggcatcag cttatcacca ccagtatata ggatcagttt
    39001 ttctccagag atcaactgat caccgttggc agtagattgt ttttgtactt ttttaccatt
    39061 accgatcact acaggttgca gtccactttt ttgagcatca gctgctgcaa cgtccgcttc
    39121 aagattacga taatcggcta cggatacttt ttctgttttt gtttctgtat ctgaatcttc
    39181 ttcagtttct ttaaaatcca ttgcccgttt catcaatgga tttgcgattt tagccaatgc
    39241 cattcgatca tatgtcttag ggtgtttcat tgttagatac aagacatagt ctggatcttc
    39301 agaaggaacc atctccacaa tcgaatacag gtaggctgta tctcctgttt gatatccccc
    39361 tgtatcagaa gcgatttggg ctgttccggt ttttgccgat acattgtatc caggtacact
    39421 atatacacca taagcactac cgtagttttc gctttcgaca acatctctca tatattcacg
    39481 gactttttct gttgtttctt ttgaaaaagg ttgccctaga acttctgttt gagttgttcg
    39541 ctcttctcca gtttggggat ccaccacttt gctgatatat cgaggttgaa tcattgtacc
    39601 gttattcgca atagaagtga aagctttcat catttggaaa ttcgtcacac ctaccgcttg
    39661 tccataagca ctcattgctc ggtcgacaat atttaatgta ggtaaagcac cgtttacttc
    39721 atcatctaat ccagaatgag tactttgtcc aaatcccagt ttttgtaaat agttatacca
    39781 tcgtccgcct aatcgctgtt ccagtatgac catcccgacg ttacttgacc atgaaagagc
    39841 ttgacgcatg gtcagcacac ctttttcacc aaagtcatgg tcattgatcg tagcatcttc
    39901 tacttggatt tttccagatt ggaaggtttc attctcgttg aattctcctt cttcgatagc
    39961 agctgcagta gtgaaaactt tcatggttga tcctggttca taactatctt gtaccaagaa
    40021 attacgccag atagcatctt ttccagtcaa tccttccatt gtttcaggat tgaaagtagg
    40081 tcgttgtccc attgccaaga tttctccggt ttttgctttc atcaggactg ctgtcaattc
    40141 ttctggctga tattcttcat ttacttgatc catcagtgtt tctaaataac tttgtagtct
    40201 gctgtctaaa gtcgtgtaga tatcttgtcc gtcgacagct ttttcttctt ctgccactgt
    40261 acctggcaga ggattttgaa aattatcttt ttgataaata atcttaccat ctttaccgct
    40321 caagatatca ttgtaagcag actccaaacc tagtttacca actaagccat tctcatcttt
    40381 gtctggaact gcgtatccaa taaaatgaga agaaaagacg ccatttggat acatccgagc
    40441 ctgatggtcg acaaaataaa gaccagcgac accttctttt ttcatgtccg cttcgatgtt
    40501 ttgcttggtt tcttgggaga tatttttccc atagctacca aattcaactt gatacagctc
    40561 tttttttgcg ccgtcctcta aaatattcag tgcatctttt ttgtctatag aaagattctt
    40621 atgcaggatt tctgctattt tatcaaaatt cttttcttca acatacagtt ttttatcccc
    40681 agaggtatat gtttttgaga gtatcgcttt aattgaataa gaggaggcat cttctgcgag
    40741 cgccacaccg ttacgatcat aaatcgttcc tctttttgct tttactactt cacttccttg
    40801 gtacaactgt tttgtttttt cagctaatga ggtaccagca acatgacctc caataacgat
    40861 atacgagaaa cggacggcaa ataaaaagaa cattccaata ctcgtagcaa ataaaatgat
    40921 acctactttt ttgcgattgt tcatcggatt tagatttttc ttttccataa accgccgaat
    40981 tttattttta agactcattt acttcacttt ccttaaattg tcgtcattaa tagacaatcc
    41041 tttttcttct gcgatttgtt taatccgttc agtacgagaa agttcgtttt tttcttgttc
    41101 taaactcgtt ttttcttgct ttttatcatt gatttctgtt tgtattgacg tgatttcttt
    41161 ttcagctccg ctgatatttg tgcgcagcat aactgttaaa acacataaag caacaatagc
    41221 aatcgccacg cttgccacag caattttttc caaagcagaa atctgcttta atcgttttgc
    41281 cggtgattgt ggaagaatga tttcttgatt cgaagaagaa tcaggcattt gttgttccgg
    41341 ctgatccacc aattcaggta aatcatattc gtaatcttgc attttcttta attctgccat
    41401 cattctactc ctctttcatt cttatttttt ctgcaatacg taactttgca ctgcgtgatc
    41461 gattgttttc tgttagctct tcctctgaag gaaggattgg tttacgagta atgactttca
    41521 gttctggttg gaattcgtca ggtatgacag gaagtcccgg tggtagatct tgtaccgtac
    41581 tatattcttt aaacatactt ttgacaattc tgtcttccaa tgaatggaat gtgattacgc
    41641 tgattcttcc attgactttc aataaccgga ttgcctgttc taaagaggcc tcttcggcac
    41701 cgagttcgtc atttactgca atccgcacag cttgaaaaat tctttttgca ggatgcccgc
    41761 cttttcttct tgcgggagct gggatgactt gtttaatgat ttcgacaagc tcaccagttg
    41821 tttggattgg ttttgttttt cgtactcgtt cgatttcacg agcaatttgt ttggaaaatt
    41881 tctcctcgcc atagcggaag aaaatcttta ctaattcatg gtaggaatat tcattaatta
    41941 cgtgataagc ggataaagga gctgactgat ccatgcgcat atccaacggt gcatcttgat
    42001 gataactaaa tccacgttct gcttcatcta gctgaggaga ggaaacccct agatcataca
    42061 aaatgccgtc taccttatct acatgctcac tcaagcgttc ttctaattct ctgaagttcg
    42121 ctttgataaa tgtgaccata ccttgttcga cataagaagc taaacgcagc ttggcgtgat
    42181 cgattgcttt ttgatcttgg tcaaacgcat acagatgccc tttagaactt agttggctta
    42241 ataaatattc actatgtccg gcaccgccga gtgtacagtc tacataaacg ccatccggtt
    42301 tgatgttcaa tccatctacc gtttctttca acatgactgt atagtgttga aatgtttcag
    42361 tcattagtca gcctcttcct attcttatag tccgaaatca atcatacttt cagcaatttc
    42421 gtcaaagttc tcttctgctg ctgcagaaaa atcttgccat cgtgtctcat cccaaatttc
    42481 aattcggtta gatacaccga tgactacgca accttttgtc aaagctgcat aatttcttaa
    42541 cgtgctgggt atattgattc gtccttgttt atcaagttcg cattcagtgg cagccgaata
    42601 gaagaatcgc acaaaagtac gtgcgtcttt tttagccaac ggcatttcgt tcagtttagc
    42661 ttccagattt tcccattcgc tcatcggata gccgaaaaga cagccatcca atccgcgtgt
    42721 cagaacaaac ttttcgccaa gttcttcacg aagttttgag ggcacgatta aacgtccttt
    42781 tgcatcaata ttatgttgga attcacccat caacattgaa agccccccat tcctccacta
    42841 actaaaatta gtctaccaca atcccccact ttctaccacc ttttctggga aatccgggaa
    42901 caactttttt tattcataaa cattttttta aaaatcaagc tgcaaaaagc cttttaaaag
    42961 actttttaca gcttttctat ttttttcata ggtaagaaag tatgttcaca atgatcaaga
    43021 cagcgtacaa gacgattgtc aacagaaaaa ccaaacgcca agtcatcttg aaataacgcg
    43081 gataaataat ttcttcataa taatatgctt ggaaaacagc aatacaaatt cccattagca
    43141 aaacagaaat aaagaaaaac ggtaagatag aaattgtccc tgtatttcta gacaatgcat
    43201 gcataccgcc ccacaaaaaa ggaatcgcca aatcaggggc ttttatttta aaacgacgat
    43261 ttaacgaaaa taattttaca agaaattggc aaccaaacaa aacgattacc gggtaaatat
    43321 accaaaacaa gataagtggt gtgaatgtgc ccatgaacgt aaactccttt tttatcgttg
    43381 cttcaagtat actgtatata ctttacacaa ctgtttcatt ttatttaaat ttatgcagtt
    43441 atattctata tcttacaaac acattccatg tattccaatt ccctatccgt tataataaaa
    43501 gaaacaggca acaggaggaa aatcatgatc aaatatttta cattagataa tgagaaactg
    43561 cgtaataccg gaaaggaaac tgaccaaacg atctggtatt gtgtagaacg tcctaatcaa
    43621 gaagagattc atcagctgac acatcaattt gaaattccgt cagattacat cacttccata
    43681 ttggacgatg cagaaaattc ccgtacagaa gaattcaatc aatcaaagtt gacaaagcca
    43741 gcgttattat tgttacaata tccttttgct aaaacaagtc ctagcggtta ttttcaagtg
    43801 gatacatacc cgctctctat tattttgacg cccaaaaaga agcttatcac aattacgaat
    43861 catgaaccac tgtttttaaa gaaagtcttt agccagacct ttccaaaaag tgatatttcg
    43921 cctaatctca atatcttctt tcaattcatt tggcaggtca cactctctta caacagttat
    43981 ttagctacgc ttcaatctca atgcgatact ttggaagcca aacttcaagt atccactgaa
    44041 aacaaacaac tttatcaaat catggatatc caaaaaagcc tcgtttattt cagagaagcg
    44101 acttctgcta atttagatgc attgaaagaa ctagcgcaaa ataaactttt ccaagaaaat
    44161 catgctttga aaaatcattt acgtgatgta ttggtggaaa cagagcaggc aatgactact
    44221 tctcgcattc aattaaaact agtagaacaa atgaatcaga ctttttcagc aatcgtatcg
    44281 aataatctga ataatattat gaaaatttta acttctttaa cgattatttt aactataccg
    44341 acaatcgtcg gcagtatcta tggaatgaac attaagttac ctattgctga aagggatgat
    44401 gcctttatat ggctagttct attaacatta tttatttgta tcgtaactac ctattattta
    44461 aaaaaaggga agtttttata aaaaagccct cttgtggaaa tggaaaaaag attgtacact
    44521 tgaatcaact aattaatgac gaggttgaaa acaatgcgtg aaaaaaaaca aacaagtact
    44581 tttgctaaag taacaaaatt tgtcatttgg acaatgctga ttttaacgat tggatctgtt
    44641 gtattgactg ctgtaatgag tgtcatgtaa aaataaaata tagctatcag ctgctgaaaa
    44701 ggacctgaac cactgcgagg tggctcaggt cctttttgcg agtttcaata gttcttttgc
    44761 atgttcgagt gtgagttttg taatctcact tccagccagc atacgtgcaa tttcttcttc
    44821 tctttctttt gttttcaact ctgaaacaga agtttgcgta cgtccatcaa tcactgcttt
    44881 cacaatataa tattgatagt cggcgactgc agcaacttga ggcagatgag tgatgcacaa
    44941 aacttgagag tatttcgata tttgagaaat tttatctgcg atggcctgtg caactcttcc
    45001 gctcacacct gtatccactt catcaaaaat aatacttgtc acaccttgct cagaagaaaa
    45061 aatagttttc aaagcaagca acatccgaga cagttcgcca ccagaagcga ctttcaccaa
    45121 aggcttcagt ggctctcctg gattggtcgt aatgtaaaat tcgatttggt caaatccatc
    45181 atgattcaat tgtctatttt cttcttttaa gaaacgtacc tcaaactcag tgttttccat
    45241 atacagactt ttcaattcat gcagaatgga tttttccaat tcttttgcga tttttttacg
    45301 cgcttcatga agatctgcgg cttgttgctg aagtagaagc tctttttgct ctagttcctc
    45361 tttcattttg tccaattgac cttctgaaaa atcagaagaa gctagctctt cggtgatttc
    45421 gtcatagtaa gccaatattg actcaatgga ttcgccgtat ttacgtttca actgtcggat
    45481 taattctaaa cgttgcgtaa cttcttctaa acgactctca tctaactcca ataaatcaat
    45541 ctgacgactc atatcgccga ttgcatcttg cagcaaatag taagcacttt ggatattttc
    45601 ataaattgct tcatactcca catctagatg ggcgatccct tgcagttctg aaacggccaa
    45661 gccaacacca tctacactgt tttgttcatt attcgtcagt gctccgtaac ctgcagctaa
    45721 tccatcagca attttttgat aattgctaag tttttctctt tcttctttta atttctcttc
    45781 ttctccgact tgcaaatcag cagcagctat ttcttcttgc tggaaatgga gcatatctat
    45841 tctctgaaca taggattttt cattttgctg cacttttcgt accctttttt ccagctcacg
    45901 ataagagata tattcttgct gataactctt tttcttctgt tggaaagcta catctccaaa
    45961 acgatccaat aaagcaagat gtgattcagg ctggagcagt tcctgatgtt cgttttgtcc
    46021 ttggatatct actaaataac tgccgattct tctcagatta gctaatgtga taatatgacc
    46081 attcactcga caaacatttt ttccagttaa ggacatatcc cgtcgaacaa tcagattgtc
    46141 ctcatctgtt tcgatcccta attcaaccat cagttcagag aatccttctt gttttggtag
    46201 ttcaaacaac ccttcaagca cacatttttc tgctccttga cgtatataat cgcttgaacc
    46261 tcttcctcct gctaacagac ctaaagcatc gataataatg gatttacccg caccagtctc
    46321 tccggtcagc gcggtcattc cttcgtgaaa agaaagccgc aattccggaa taatcgcaaa
    46381 gttagtaata ctgatttctt gtagcatcac attcgctcct caacttaaag gtaatgcagg
    46441 aagtctttct tcaataaaac tgcctggtct tcagttcgtg taatcatcaa aacattatcg
    46501 tcatcattga taatcgaaaa taattctttc ttgtaacgtt tatcaatcaa atttgctgct
    46561 gccgaagcat ttcccggcaa tgttttcaaa ataacaaatt tttccatttg gtcaactgcc
    46621 acgaaagcgt cttttaatag tttttccaat ttagtattta catcttcgtt tgtttcagct
    46681 ggtatgctat aacggtagcc gccatctgaa gccggtacct tgatcagttt cagttccttt
    46741 atatctctgg aaatcgtagc ttgagtaaca gatattcctt tgcttttcag gatttcaaca
    46801 aattcttcct gctttcggat atcttgctca ctcaacaatc gagtaatcaa acgatgccga
    46861 tctttttttc tcattacgtc ttcgctcctt agcatattct tcttaatcaa tgatagctta
    46921 tttccttggt ataataaaga agaatccgca tacttctcac ttgattttca cagttacaat
    46981 ttatttatgg tcaaactgtt cgtgtgcatt tgaaaccact tctgccatat taatagctga
    47041 attgattgtt ccgctttcgg gtactttcct caagtgagcc aagaactcga tatttccttc
    47101 tccccccgtg atcggagaat aacttaattc cagcacatca tatccacctt gtgccgcaaa
    47161 tgacaaaatg tcttctaaaa ctttttgatg ggtctgaggg tcacgaacaa ttccgttttt
    47221 ccctacagct tctctgcctg cttcgaattg gggtttgatt aaagcaatca cttctccctc
    47281 ttcttttaaa atagcatgta atggaggcag catcagtttt agtgaaataa aagaaacatc
    47341 gataacagca acttctggaa ttcctaaggt aaagtcttcg ggttttgcat aacgaaaatt
    47401 cactctttcc attacgacga ctctaggatc ttgtcttatt ttccaagcta gctgattgta
    47461 gccaacgtct aaagcataac tcatttttgc gccattttgc aaagctgcat cagtaaatcc
    47521 gccagtggat gcaccaatat ctaacaatgt ttttccttca acagatagat caaatgcttt
    47581 gagtgctttt tctaatttta atccccctcg agaaacataa ggaagttttt tacctttgac
    47641 tcttaattca ctactcactg gaattttttc tcccggttta tcaaaccgct cgtttttttc
    47701 gttgtaaatc aaaccagcca tcactgaacg tttggcttgt tctcttgttt caaataatcc
    47761 ttgtttgacc gctaatacat ctacgcgttc tttttccatt ctttccctca tttctccaca
    47821 tcaaattgct tgatcatctg gcgcatcagc ccgccatcaa aagaaaaaac gtcctcttct
    47881 aatttgtcta tgatcttgtt ggcggataac agctcaatct ccaacgcctc acgtgtcttt
    47941 tctaggccta ataaacgcgg gtaagtattt ttttccatac gttcatcttt accagctgtt
    48001 ttccctaatg ttttcgttgt accaatcaca tcgagtagat catctcttat ctgaaaagct
    48061 aaacctaaat gttcggctaa tcgttgaagt agcaataaga tttcttcagg ttgcttcgct
    48121 aatatgccac cagccagcaa ggcataacga atcaaatgcc ctgtctttct ttcatgaata
    48181 aatgctaatt cttctaaaga aagctttttc gattcgccct cgatatctgc agcttgccca
    48241 gcaaccatac cttgtgatcc cgcacaaaca gctaattgct gaagcagcag aagcttagga
    48301 gaattaccta agtgtgccat actgattagc tggaatgctc ctgtaagcaa accatctccg
    48361 gccaatatag ctaatgcttc gccgaatact ttatgattgg taggttttcc tctacgaaga
    48421 tcatcattgt ccatcgcagg caaatcatcg tggatcaagg aatacgtatg caccatttct
    48481 aaagcagctg ctacttgata gacgggaaca tcaataggct catcaaagga agcaacggcc
    48541 gttaaaagta ataagggacg tattcttttc ccgcctgctt tcactgaata actcatcgca
    48601 tcatagagtc ctctatccgt tgtatgctca tctaaaaaag acaagatttc tttttctaca
    48661 gcaggtagat gaatagctga aaagtcagtt aatctcatct cagctttcct cgtttccgtc
    48721 aaaatcaact tcttcatttg attctgtcat cattttcgtc aaggtctctt ctgctttagc
    48781 taatgtatct tggcaatgtt tgcttaattc cattcctcgt ttaaacgaat ctagagctga
    48841 ttctaatgga acgtctcctt gttctaactg catgacaatt ttttctaatt cttgcagtga
    48901 ctcttcaaaa gttggatttt ccatattttt attctccttt gtctatctct tgtacttgtc
    48961 ctttaacgat tccatcttga taatggatcg tcagcaaatc cccttgatga atctcggtga
    49021 cactttttac tacttgatca tttaacgtcg tatagctgta gcctcttccc atgattttca
    49081 atggactcaa caaatcaaga gattgaacag cttgagcaaa acgctgctgc tgttgtttca
    49141 tatattgttg gattttctct tctaagcgtt tttctagata agcagtttct tggcgagctg
    49201 ctttaactcg ataaagcgga gccacttgtt ccaattggtg gcttaatgcg atcgtttgtt
    49261 tttctttcgc atgataaatt tgctgtgtag cttgatataa tcgctgtttc aactggtcta
    49321 atttgatggt ctgaccttca tacaatcttt caggctgccg aaatacatag gaacgtcgag
    49381 cacgctcgaa ccgttcttgt ttgcgctgga tctggcgtac aaacgcctgc tgcaatctag
    49441 cttgccgttc ttgaatgcgc atcaactctt ctgacaaaac aggaacagct aattctgcag
    49501 ctgctgttgg agtggcagcc cgaacatctg ctactaaatc agcgatcgtg gtatctgttt
    49561 catgtcctac agatgaaatc accggcgtat tagcttgata aatcgcccga gcaacccgct
    49621 cttcattaaa cggccataaa tcctcaatcg aaccaccccc acgaccgatg atcattgtat
    49681 cgaaattgcc catctcttct actctctgga tattcctgac aacattgtcc gctgcttggt
    49741 ttccttgtac aactgttggg aacaaaacca attggacaat tggatatctt cttttcaccg
    49801 tcgtgatgat atcgcggata accgctccgc ttggactggt aaggaccgcg attcgctttg
    49861 ggaaacgtgg cagcattttt ttaggccctt gaaaaaggcc ttccttcatc agcttttctt
    49921 ttcgttcttc caattcttga tataaagcac ctattccatc aggctccatg tgctctacat
    49981 agatttgata agaacctccc gcttcatata atgaaatacg cccaacgaca aggactttca
    50041 ttccttcttt tggctggaat cgtaattttt ggaatgctcc tttgaacata atagcagata
    50101 tttttgcatg gtcatctttt aagctgaaat attgatgggc attggcacgt aaacggaaat
    50161 tcgatatttc gcctgttaaa tatacgcgtt ctaaataagg atcggcatca aattttcttt
    50221 tcaaatactt cgttaatgta gtgacagtta aatattcttg tgccatctat tcacctgctc
    50281 caaaagcttc ggcagatttt acggtttgat acatcagcat cgtgatagtc attggcccta
    50341 caccctttgg cacaggagtg atccagcctg caataggctc cacttcatca aatttcacat
    50401 caccgatcag cttaccgtta gcatcacgat tcatccctac atctatcact acagcacctg
    50461 gttttacgaa ttcttttgtg acaaaatgtc cacgtcctat tgctacgact aaaatatctg
    50521 cttcttgtgc cagttcagcc agattttcgg ttttagaatg agcaacagta acagttgcat
    50581 ctgccatcaa gagtagctga gccatcggtt taccgacgat attgcttcgt ccgataacta
    50641 ctgctcgttt acccgccgga tcgatagaat aagcttcaaa cattttcatg atgccgtatg
    50701 gagtacaagg aatcttgtcc ggtttacccg caaggagtct gcctaaattc atcggatgaa
    50761 aaccgtctac gtctttttca gggcggatag ctaacaggat cttttcttca tctatatgct
    50821 ttggcaatgg taattggacc aatataccat gaaaactagg atcttgattg tatttttcaa
    50881 tttctgcaag tagttcgtct tcagaaatcg tctcaggata acgctctacc aatgaatgga
    50941 ttccgatttc ttttgctaca cgttctttat ttcttacata gatttggctg gctggatttt
    51001 cccctaccaa taaaacgact aatccgggat ggataccatt atctttcaat ttttggattt
    51061 tttcagcaat ctctgcctgc attttttcag ctaattcttt tccgttaatc aattctgcca
    51121 tctaaccact cccagatatt ttttgtgttt tcattctacc atagaatttt cccgatttat
    51181 aacaggttta tgatgattca ta
//
LOCUS       DAYYGY010000011        45582 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000011 DAYYGY010000000
VERSION     DAYYGY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.011"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACHA52_000690"
     CDS             6..245
                     /locus_tag="ACHA52_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939440.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACHA52_000691"
     CDS             312..521
                     /locus_tag="ACHA52_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939441.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACHA52_000692"
     CDS             581..1252
                     /locus_tag="ACHA52_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8939442.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACHA52_000693"
     CDS             1305..3281
                     /locus_tag="ACHA52_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8939443.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACHA52_000694"
     CDS             3294..4049
                     /locus_tag="ACHA52_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8939444.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACHA52_000695"
     CDS             4046..4870
                     /locus_tag="ACHA52_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8939445.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACHA52_000696"
     CDS             4880..5128
                     /locus_tag="ACHA52_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939446.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACHA52_000697"
     CDS             5171..7270
                     /locus_tag="ACHA52_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM8939447.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACHA52_000698"
     CDS             complement(7308..9365)
                     /locus_tag="ACHA52_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8939448.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACHA52_000699"
     CDS             complement(9479..9691)
                     /locus_tag="ACHA52_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939449.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACHA52_000700"
     CDS             9878..10345
                     /locus_tag="ACHA52_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939450.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACHA52_000701"
     CDS             10342..10584
                     /locus_tag="ACHA52_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFM8939451.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACHA52_000702"
     CDS             10602..10904
                     /locus_tag="ACHA52_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939452.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..12943
                     /locus_tag="ACHA52_000703"
     CDS             10976..12943
                     /locus_tag="ACHA52_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939453.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRLEQEKEKEAAARKERQE
                     QMDTLRQAQLNYLAQPQPVAAVQTPVNNQANLTPSPQRSFNGWMVGCIASLSLLLGGG
                     TVFAFNHINQSSQADSVATTASIKEEAQEQARKEYEQQLEELLNRTNQSTTESSMENS
                     KESDSSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            12943..15555
                     /locus_tag="ACHA52_000704"
     CDS             12943..15555
                     /locus_tag="ACHA52_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8939454.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15552..18083
                     /locus_tag="ACHA52_000705"
     CDS             15552..18083
                     /locus_tag="ACHA52_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8939455.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18135..18467
                     /locus_tag="ACHA52_000706"
     CDS             18135..18467
                     /locus_tag="ACHA52_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939456.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18468..19061
                     /locus_tag="ACHA52_000707"
     CDS             18468..19061
                     /locus_tag="ACHA52_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939457.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19224..20918
                     /locus_tag="ACHA52_000708"
     CDS             19224..20918
                     /locus_tag="ACHA52_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFM8939458.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21606..23483
                     /gene="ltrA"
                     /locus_tag="ACHA52_000709"
     CDS             21606..23483
                     /gene="ltrA"
                     /locus_tag="ACHA52_000709"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8939459.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23614..23991
                     /locus_tag="ACHA52_000710"
                     /pseudo
     CDS             23614..23991
                     /locus_tag="ACHA52_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            23991..25196
                     /locus_tag="ACHA52_000711"
     CDS             23991..25196
                     /locus_tag="ACHA52_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8939460.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25212..25856
                     /locus_tag="ACHA52_000712"
     CDS             25212..25856
                     /locus_tag="ACHA52_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939461.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25867..26673
                     /locus_tag="ACHA52_000713"
     CDS             25867..26673
                     /locus_tag="ACHA52_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939462.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26695..26925
                     /locus_tag="ACHA52_000714"
     CDS             26695..26925
                     /locus_tag="ACHA52_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939463.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27471..27746
                     /locus_tag="ACHA52_000715"
     CDS             27471..27746
                     /locus_tag="ACHA52_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939464.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27788..28210
                     /locus_tag="ACHA52_000716"
     CDS             27788..28210
                     /locus_tag="ACHA52_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939465.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28229..29821
                     /gene="mobP2"
                     /locus_tag="ACHA52_000717"
     CDS             28229..29821
                     /gene="mobP2"
                     /locus_tag="ACHA52_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM8939466.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29833..30168
                     /locus_tag="ACHA52_000718"
     CDS             29833..30168
                     /locus_tag="ACHA52_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939467.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30315..30509
                     /locus_tag="ACHA52_000719"
     CDS             30315..30509
                     /locus_tag="ACHA52_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939468.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30541..32913
                     /locus_tag="ACHA52_000720"
     CDS             30541..32913
                     /locus_tag="ACHA52_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8939469.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            32974..34434
                     /locus_tag="ACHA52_000721"
     CDS             32974..34434
                     /locus_tag="ACHA52_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8939470.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34445..36649
                     /gene="topB"
                     /locus_tag="ACHA52_000722"
     CDS             34445..36649
                     /gene="topB"
                     /locus_tag="ACHA52_000722"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8939471.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36665..36814
                     /locus_tag="ACHA52_000723"
     CDS             36665..36814
                     /locus_tag="ACHA52_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939472.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36888..37481
                     /locus_tag="ACHA52_000724"
     CDS             36888..37481
                     /locus_tag="ACHA52_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8939473.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37497..38396
                     /locus_tag="ACHA52_000725"
     CDS             37497..38396
                     /locus_tag="ACHA52_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8939474.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38399..38884
                     /gene="ssb"
                     /locus_tag="ACHA52_000726"
     CDS             38399..38884
                     /gene="ssb"
                     /locus_tag="ACHA52_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8939475.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39250..39510
                     /locus_tag="ACHA52_000727"
     CDS             39250..39510
                     /locus_tag="ACHA52_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939476.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            39951..40157
                     /locus_tag="ACHA52_000728"
     CDS             39951..40157
                     /locus_tag="ACHA52_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8939477.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40157..40408
                     /locus_tag="ACHA52_000729"
     CDS             40157..40408
                     /locus_tag="ACHA52_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939478.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40423..40860
                     /locus_tag="ACHA52_000730"
     CDS             40423..40860
                     /locus_tag="ACHA52_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939479.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40853..41560
                     /locus_tag="ACHA52_000731"
     CDS             40853..41560
                     /locus_tag="ACHA52_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939480.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41725..41925
                     /locus_tag="ACHA52_000732"
     CDS             41725..41925
                     /locus_tag="ACHA52_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939481.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            41941..42120
                     /locus_tag="ACHA52_000733"
     CDS             41941..42120
                     /locus_tag="ACHA52_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939482.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42461..42724
                     /locus_tag="ACHA52_000734"
     CDS             42461..42724
                     /locus_tag="ACHA52_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8939483.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42718..43068
                     /locus_tag="ACHA52_000735"
     CDS             42718..43068
                     /locus_tag="ACHA52_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8939484.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43092..43706)
                     /locus_tag="ACHA52_000736"
     CDS             complement(43092..43706)
                     /locus_tag="ACHA52_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8939485.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44020..44442
                     /locus_tag="ACHA52_000737"
     CDS             44020..44442
                     /locus_tag="ACHA52_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939486.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44452..44655
                     /locus_tag="ACHA52_000738"
     CDS             44452..44655
                     /locus_tag="ACHA52_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939487.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44866..45450
                     /locus_tag="ACHA52_000739"
     CDS             44866..45450
                     /locus_tag="ACHA52_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8939488.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16703 a         7199 c         9245 g        12435 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatattttt ccaaggagta gagaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactttcac cgtgagtgac gttgattatc tgttctttca aaatatcaac tcgcccctca
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcaccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtca cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatggtaa ccatatattc ttttggcgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattcggtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatatttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tggacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggtt agagcaagaa aaggaaaaag aagcagctgc
    12481 tcgtaaggaa cgtcaagaac aaatggatac attgcgtcaa gcacagttga attatctagc
    12541 acaacctcaa ccagtagctg ctgttcaaac gccagtgaac aaccaagcaa atttaactcc
    12601 ttcaccccag cgttccttta acggatggat ggtgggatgt attgcaagtt taagcttgct
    12661 tctaggtgga ggaacagtat ttgcttttaa tcatatcaat caatcttcac aggctgattc
    12721 tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa caagcaagaa aagagtatga
    12781 acaacaatta gaagaattat tgaatcgtac caatcagtca acaacagagt caagcatgga
    12841 aaatagcaaa gagagtgatt ctagtaaggc taaagaagga tcaagcgatt caactgtaca
    12901 atcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa taatgcgtgt tagagaaaca
    12961 acgaagcgaa aagacaaacg attcaattta gaaaagaata ggtatctttc tcatttctat
    13021 cgctatactc cctcaggaga agagctacca gaaaaacatg acaaacgcat ttcgaaaaag
    13081 attttttata gtggacttgt atacctccct ttagagtatt tagtaacaag agtaccagaa
    13141 gttttcaacc gattggaaat tgttaaggga agcttacaag gaataaatct gtttcaatta
    13201 caggaaatgc cacaggtctt tcattttgat tcagctggtt tttggaatcc atttactcct
    13261 acaactagtt tgggtacacg tttagcaatt tcgacagttt gtgtggttcc agctgtactt
    13321 atgggattga aaatggacat gtggtttcgt cctctagcag atggacaaaa aggggataat
    13381 cgattaatga ccttaaaaga aatccaacag atttatccgc aaatacctga aacaaaaact
    13441 acttttccag gttatggagg tattccaatc acacattaca aaaaatattg gtacattcaa
    13501 acagatactg ttaatacgtg tattgttggt acctctcgat cagggaaagg tcagattgaa
    13561 gtgttagcca ccattgacaa tttatctcgt gcagaaaaac aaagttcaat ggtcgttaat
    13621 gatccaaaaa cagaattata tgttggtagt aaagatgaac tagaagctag agggtatgat
    13681 gtatatgcct ttaatatcac cgatccactg caatcaatga gtgataatcc attggcactc
    13741 atcgtgaaat actggaaacg aggagacata gatactgcaa cacagttaac caatactttc
    13801 acaaatacga tttattatga tgccaacgct tcagataaca aatactttaa cgaaaacgcg
    13861 cagaaagctg tcaatgcgct tatttttgcg ttgttagaac aagctgatca aacaggagat
    13921 tactccaaat tgacaccgaa taatctcgtt gagcttcttt ctgagattgg cggttttaat
    13981 tattatccag atccagacaa tgaattcaaa cagttgaatg ccttagatga atttatgaat
    14041 cagttgccac ctggaaacat tgcaaaaaaa caatatgggg caacgaaaat tggatctgat
    14101 aaagcgaagg gaaatatttt atcaacggca atcacgggat tgaatccatt tactctaacg
    14161 aaaattgcaa agatgacttc tcaatcaacg attgattata aatcggttgg gtttccaaaa
    14221 tacttagatt tgaaattaca tgaaagtctt ttaaatcaac gagtcgtagt agaattttat
    14281 cataagggaa agaaatttca tgaagaacgt gtcaaagtag gctataaagg atttagtgaa
    14341 atcaattttg attgtcattt agttgcaggt gatcaagttt gtttaaaata cgatcatcaa
    14401 ggaaaagaat atgtggcttg gtatgaattt tctcaaagag tattgaaaga cgaagagggt
    14461 aatatcgttt atcggaaaaa acgaggagaa acccatttgc cagaattaga aaaagaagcc
    14521 gttctaacat tagatgagga aaaaagtaac ttattagtag agtctattcg gatgcactat
    14581 accgataaac caatggctat ttttatgctt acacctgatt atgatccatc taaccatatc
    14641 attgttagta tttttcttag tcagttatat aaagaattat ctacgcaatg tgttaagact
    14701 aaaggagata aatgtcaccg caggattcat tttattttag atgaatttgg gaatatgcca
    14761 ccaatggata atatggaagg aatcatgacg gtcacagctg gccgaaacat gttgtgggat
    14821 ctgtttattc aatcttatca gcaactttat gcgaagtatg gagaacaaaa cgcagcgatt
    14881 atcaaagaaa attgtcagac acatatttat attatgtcga cagatgataa taccatcgaa
    14941 gaattttcta aaaaagtggg gaataagacg gttgaacaag agaacagtac catcaattca
    15001 ttaggtatga atactcatat caatcgcaat gttgattcag atcgtatctt aactcctgaa
    15061 agaatttcta cttttttaga aggagaaacg attgtactcg ctcctttaaa acgacaagat
    15121 ttgaaaggtc ataaaatacg gccgtatcct atttttaatc atggggaaac gaatcttcct
    15181 tatgcttatc aatatattga agatttcaat ccatcgtggg atatcaatga tatagacata
    15241 cattgtacac atgcggactt ggatcttaag gctttacaaa ttgattatga ttatttccta
    15301 ccagatgaat atgctagaaa tctctatcat ggactgcatg atcaattaga agaaaaaaac
    15361 gaagaaaata tgttttataa aatcgttcaa tctcttgaga atgagacggc taaaaaattg
    15421 ttaattcaat tttacgaaga acaagcagca gatcgacttc aagaatatgt tgaaaagttg
    15481 atcaaaaata aagaaatttc tgaaatgatt gggaaacggt tgttattgga atctgaaaag
    15541 gtggtgaaag aatgatcatt ggaggaattt tttctggcat agccgactgg tggtcaggct
    15601 tattcggaga aggggatgca aaccccaaaa ccgtaacgaa agtcttacaa gattatggga
    15661 attatctgca gtatcaagat gtatttagtt ggatttggaa cacttttctg tggggattga
    15721 ttaaaggatt atataatctc aatactttac tagaaaaaac catttatcaa agctttgatt
    15781 taaaagactt attgaacgcc gctggggtaa atgcactatt tcaaagtttg attagtaagg
    15841 tattggctct tttttgtgtc gtcacattaa tttatttagg cttgaaattt tttatgagta
    15901 aacacccacc aaaagtcaaa aatatcttgg ttaatttatt tatggccatg atcttgattt
    15961 ttggtggtag ttctttaatc gatgaagggt taaatatcag taaaagcttt tacggagacg
    16021 tcactgcagc aaataaagca gataaaacgg gatcacctgc ttttcaaatt attcaaaata
    16081 atgtgatcga tgtcagtacg ttactagcaa ctgacccgaa taaagtagaa tccctaccaa
    16141 cagataaaag aaatgcttta acagcgaaaa attttaaaac agcgaatatc aatgggatta
    16201 ttactcctga acaagctaaa ggtatggcag gtaatgataa aagtgggtta tcagatatac
    16261 aaaaggatcg gttaaaggca ttacaatacc gattggaact agatgataat gggaaagaaa
    16321 ttcctgttca aattagtgac gaaggcttag caaaatatgt ctatacttca gggtatcgtc
    16381 gatacagtag taaaccaggt attattttag ctggtgaatc ttctttggca gtagcctatt
    16441 tatttatcct atttacgatc gttacttgca ttattgagct ggtatttaaa aaattctatt
    16501 tagtgattgc cgcgtcaacg gactttgaga caggtcaacg gatgaaaagt gcgatagaag
    16561 atatttctca atccttcttg ttattggcgt ttacgatttt agaattacga atctatacct
    16621 taatgctttc aggtatcggt gatttgcatg cggcaggaaa gatcaatgga ttcttgtatg
    16681 tcgttgcttt gattgtacta accattgcgc tattcaaagg ctctcaatca gttacgaaaa
    16741 ttttcggcgt tgatacaagc ctgaaaaacg gcagtaattc tcttcttagc atgtttgcct
    16801 taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa ccttgcaagt gctggtaaga
    16861 gtggtttaag ccatttaaat gatattcgta aaaatggatt tggccatcga aaagatgtta
    16921 cccctgataa cggggaagaa caatcagacg ataaagctac aactgtacct aatagtaaga
    16981 aaacagtttc cgaaatgttc gggaaagcga aaaatggtgt ttctaaagcc gcagaaggtc
    17041 ttggttacgt caatgaacgt gggttaaaag gaacagctga agatcttaca gaaaaagcca
    17101 aagacacctt agaagataaa ttagacggca cgagagctaa agaattggct gaagctgtga
    17161 agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc ttcagaagtc aaagaaggac
    17221 taaaagatgc gtatggtgaa ggacaagtca atgcagcagt gaaagctaat acaacaaccg
    17281 aaaaagaaaa tcaagaaggt atagtgattc cttcagtcaa agatcaccaa gaagcgttgc
    17341 actctaatga aaattcgatt gaagagaaaa attccataga agaaagaaat tcagaagaag
    17401 gaaaaaatgg acagttacca aaaactgatc cagaacaata tcgctataaa cctaacagtg
    17461 accttgaaaa tgagaagcga atttcagatt tatcaaaagt aaatcaagaa gacaaagcag
    17521 aaaatgaggg acagttacca aaaactgatc cagaacaata tcggtataga tctgttaact
    17581 ccgaaaatga aaaacgcatt tcagatttac caaaacgaaa tcaagaagta caaacggatg
    17641 aggattccat cgttctgcca aatgataatg gaatcgaagt tggaactgaa aaatcaacga
    17701 aaattggttt acctaaggca caaaatttac caatgaaaga tagtcgtttg aatcaattca
    17761 aggataatga tcaaagtttt gttcatcaat cacctgcagg tactgttcaa atgaaaccaa
    17821 gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta caatttatct gaaagtggtg
    17881 agacagggaa tgcgtccgtt caaaatgttg tgacaagaga agctgcacca aaacctattc
    17941 aaaacgttca tacaggttac aagccagcaa atgtggcatt tactgcacca acaaaaataa
    18001 atgtgacaag agaaacaaaa aacaggaaca atgcattgaa agaacaacta ttgagggata
    18061 tcaaacagga tatcaaacca taaaaggcct acaagttagg ccttttttct atgtatgaaa
    18121 aggagaaatc atttatggat aaatatagta acatgaaaaa ccctccaaga tttagtggag
    18181 aatggaagtt tttcgctttt ttctttttag atctattaac gcttggtggc tgcttttttt
    18241 gggcgaaaat ggtcaataat cttgtccact taccactctt ttttgcactc ttgaattatg
    18301 gcttatggat tgcgatagcg ttagtgattg tttggcgacc ctataataac ccaggtcaac
    18361 gacaaatttt tgcgattatc gagtctattt tttttgcgga caagaaccat tatcatgcgt
    18421 tagatcctaa ccgaaattat catggtacgt ataaggagga tgaataaatg cgagtcaaag
    18481 gacaaaattc agttgaaagg aaatccattt tgcagtttgg aaaaccagaa gagcgctcta
    18541 tcttggactt atttccatat aaatcctgta cagaagaggg agtcttaatt actagagaag
    18601 atcattttca acgttattat cgtgtagttt ctacagacgt ggaaggatta aatgaacaag
    18661 agaaactaga acgaatgaat cagttgacca ccgtcatgcg tacgtatgtg ccaacaatta
    18721 agttagttag tttgaccaca gagacggatc taacggaaca aatcgtgcaa aagcggcaat
    18781 tattagataa aaatcgtatg gaacagtcct taggaagaaa tctacaacga ttaaaaaaat
    18841 atgaaaagaa attagtcgaa gaaatccagg aattgaaacg agcagaacaa gaacgtcctg
    18901 acttaagctt ctttttcgtc attgaaggaa agacaatcaa ggatttacaa accagaaacc
    18961 gtcagttaat gcgatctagt ggcattttag ggttaaaacc actctacaaa aaagaattga
    19021 tcaagattct gtatcgaatg aacaatatga atgatgagta ggaatggaga agagagaatg
    19081 aaatatccgt tgatatggtt agaaaaagat gttccgaagg aagagaccac tagaaaattt
    19141 cctcacactt taagcctggg taagttggga aaagggcaat cttttaacca agtaaagaat
    19201 caaacaagaa gaggtgactg tacatgttag aagcaattaa aacagctttt tcaccacaaa
    19261 atgatcaaaa agtacttgat ccattaaagc aactaggaaa tcgggaaata gaaattgtaa
    19321 aatccaaagg gtatgattta gattttttaa aacaagtgat gccacaaggt gggttaaatt
    19381 ttgatgaaga atatgtgacg tatggtaatg gttattcacg atgtgtgaca gtttatcact
    19441 acacaaaaaa tcctacgctt ttttggttgg ccaatctcat gaacaatcct catacagtgg
    19501 ccacactcga tgttagcaca gatgaaaagc aaaaagtgat tgatcaaatc aatcgtgcct
    19561 tagatgaatt aaatgatcga tcagataatg aacgaaaagc gactgaccga aataaaagcg
    19621 cggaagaata cctggaacta gcggaatatg ctagaaaatt gacttctaat ggggaaattt
    19681 ctaagcaagt caaagtgaga gtacatgttt acggaaacac gattcaagaa gtagatgaac
    19741 gagcgcagga actgatcgaa aatctcaaag ggatggatta tcgtgcggtg acttatctgc
    19801 ataaaaccaa acgagattgg caatcgatgt ttaccatgtt cgatgaacaa gctgaatggt
    19861 tcggaaatat caaagggcaa tcactaccat cgttaaacat tggcggaggt ttcccgttcc
    19921 accaccaaac gctaaaagat ccaggaggga cacaatatgg ggaaaccatg acaggcggcc
    19981 catttgtctg gaatgcgacg tataaaaata gtgtacggtt gtctactaat accttgcttc
    20041 taggaatgat gggagctggt aaatcgaccg ttcttaaaat gatcgcggaa gcccatttag
    20101 ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga ctttattcct ttcttaaagg
    20161 aatataacgg tattatggtg cgtttagacg gatctgacgg aatgatcaat cctttagaga
    20221 tatttgccac acggacgtat gatgaagcat ccagtttata tgacgatgga tcagtcaaag
    20281 atttgaagat caatgaagca gcgtcctatc aaacacactt agacaaagtg gtctatcagg
    20341 tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc agaatttaag acctacttaa
    20401 atcgttttta tgaagagtat ggaacagtac cacgcggctt tacttcctca aattcgcgtt
    20461 caaatactga tacgcaagtc acagggaaag atccagaagc ttatccgacg ttcaaagaat
    20521 ttttagactt tttaggacaa ctggaattac caggtgcttc ccaagagaaa aagaatcgaa
    20581 aagaagaaat ggaatccatc gtagaatcct tatgtgagac gtatggcatg atttttgatg
    20641 gtcatagtac gattcgacac cttgaccaac aacagttggt cttttttgac attgactcta
    20701 ttacaggctt agatcgaggg gtacaacaat gtcaaatgta catggctatg acgttgattt
    20761 ggaatcaggc attgattcaa ggaagaagac aacgacgttt aattaaagaa aaacaaatgg
    20821 aagaacaaga cttcaaacgc ttcttggcga tgattgatga gtgccataat ggtgcgacct
    20881 gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa actattgtaa taactcttat
    20941 aacgagaagt caaatgagtg ggtaacgcca caaagggctt ccactgatac tccgacatgc
    21001 aacaagtagt ttatgcagac caacgttgtg tgaagctcgg tgaagtcggc tgaaggatac
    21061 cgtaaggtaa aagaaatagt gaataccacg aaagttctct agaggtaaag ggtgtatccg
    21121 ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg tacaaacgta ctgacgaact
    21181 agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg gagagacagt gagcaaaaaa
    21241 tcactcattg tgctaacttg tggttgagta aaagtcgttg ttatgaaaga ccatgctgcg
    21301 ttacaggcgt aggagaagtt agggggcaaa agctagcatc taagttaagg catgtggata
    21361 gatataaggg aacaggggaa gccatcaaca tggagagtgc atactcgatg aagtggtagt
    21421 aagaaatatg tgaatgacaa ataacttact ttagtgatgg tgaaagtaga gtcacgactg
    21481 atgaaggttc tgtaatggaa ctggaggaac aggctcgatt cgtctgtaaa agtcatgaaa
    21541 cccgttcaac catgggattc tcgtatgact aaaaaggtca cgagccaatt ggagcgtgat
    21601 aaaccatgac aaaagaaaca agtccaaaag ataggaagtt gcggcatgca gaatattatg
    21661 gcatgacgga aatcttcgat aagatatact ccaaaagttt gaagggagat aatttcaaga
    21721 atctaatgca gattatcatt tctgataaca acattgtcct agcatatcgt aatatcaaga
    21781 gaaatcacgg tagtgtgaca agaggagaag atggagtaac gattaaagat attgaaaatc
    21841 tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt cgcacattat aaccctcgga
    21901 aagtaaggcg tgtagaaatt cctaaatata atggtaaatt aagaccgttg ggtattccat
    21961 caatatggga tagaatcgcg cagcaatgca tcttacaagt attagaacca atatgtgaag
    22021 caaaattcaa taatcacagc tatggtttcc gcccaaatag atcagcagaa cacgcaatag
    22081 cagattgtat ggcccgaatg aatcaaagtc acatggaata tgtggtagat gtagatatca
    22141 aaggtttctt cgacgaagtc aatcatacta aactcatgag acaaatctgg tctatgggta
    22201 tacgagacaa acagcttctc ttaatcatta ggaaaatgtt aagagcaccc gtagtacttc
    22261 caaatgggaa gttgatatac ccaacaaaag gaacaccaca aggcggaata cttagcccat
    22321 tacttgcgaa catcaatctc aacgaatttg actggtggat agcaaatcag tgggaggaaa
    22381 aagatgccaa agagctaaaa catcatttta ggaaaaatgg agcttgggat aaaggtaata
    22441 tctatcacaa gctcagaaag agtacaactc taaaagaaat atacctagtg agatacgcag
    22501 atgattttaa aattttctgt agaaattata atgaagcgaa aaaagttttc tatgcaacta
    22561 aaatgtggtt acaggagcgt ctaaagttac caatatctga agaaaagtca aaaatcacaa
    22621 atctttcaaa agaagaaagt gaatttttag gctttacgat gaaattagtg accaaaggta
    22681 acaaaaaggt tgttaatacg catataggta caaaagcaca aagcatgatt aaagtgaaac
    22741 taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag caaagatacc tttatctata
    22801 ttggagtcta taacagtatg gttattggga ttcataatta cttcaaaatt gcgactcatt
    22861 gtagtgtagc aatgcgaaaa attgcaagag aactcagacc aagttttgaa atacgactac
    22921 gagaacaagg aatctcaaaa gaaggacaat acggaaagta cgaaggatac aagccttacc
    22981 tacggtcagg acaaatacga tatataagag ggtttccaat attaccagta gggtatattc
    23041 aacacagaca tccgatgtgt aaaaaaagaa atattaataa atacaccgaa gaaggtaggg
    23101 aagccattca taagaagcaa caaaccgtag aagaatggaa gatatcgtgg ttgtgtagca
    23161 atccagtgct tagtaagaga gcaacgattg aatttgcgga caatcgaatt tcaaaattca
    23221 tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct tatcttatct gaaatgcatt
    23281 gtcatcatat tatcccttat catgaaagca aaagtgatag ctatgaaaac ctagtgattg
    23341 ttactgagga ggttcatcgg gtaatacacg ctacacaaag tgaaacgatt gaggaacttc
    23401 taaaatatct aaaattaaat ccaaaacaaa aagaaaaact caatgagttg agattaaaag
    23461 ttggtaacga agaaatatct taataaatat ttgtaggacg ggaaaacaga atgtctttta
    23521 tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac ggtgtggact gggggaaaag
    23581 acggagataa tatcaaactc ttacctatca gtatcgatta atccagattt tattgaaacg
    23641 gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat gactgtttta
    23701 gctacacaat ccccacaaga aatggtgcca gaaaatatgt cttctgataa cttctcgaaa
    23761 ctgaagaaag tctttgaatt ttcttctacg aagatcttta tgcgtatgga tgaatctatt
    23821 ttacgtcata tagaaggatt agtgggtaaa tcaatgacga actcagaatt agaatccatt
    23881 cctcaacaaa atcaaggaga cgctgtgatc aattttggct caaaagaaac cattcgtgtt
    23941 cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc
    24001 aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc aaagcggcgc ttatttttgt
    24061 ggttgtttgg tacttctgca ggtttgatta ctattttgat cactgtgttc gtgaccctat
    24121 ttctaatact tgcggcgggc tcgattgata attcggattc agattcaagc agtggtggcg
    24181 aagcttttac aggagaatat tcggaaggct taccgattta taaagaaatc aaaggaagag
    24241 gccctttttc tgatgagatt gcgcaatatg ccgtaggtgc agcagtgaaa tataaattat
    24301 taccaagtgt gatcctttcg caatacggtt acgaatcagc gtttggtaca tcggcgtccg
    24361 caagaaatga tctcaactat tttgggatta cttggtttga tggttgtctg tttcctaaag
    24421 gaactgcacg aggaattggt ggcattgaag gtggctggta catgaagttt cccaactcaa
    24481 aagctgcatt ttcctattac ggctttatgg tagccacaca atccaatttt aatgcttgtg
    24541 taggaaataa aagccctggc gcaagcttat tgatcttagg acgaggcggt tatgccgctg
    24601 caggaattac ggaagatagt ccgtattata caggttgtat gtcaattatc acttctaata
    24661 aattaaccga atatgatgaa ttcgccatca aacattgggg tgaaggtggc aataacaacg
    24721 gcacgattac tggtgaatgg acgaatccat tccctggcag cagtttagat aaaagttcct
    24781 tcagcggtgg tcaattattt ggaacgaatc caggcggtga attccgtccg aacggcttcc
    24841 atgatggatt agattttggt tcagttgatc atccaggaag tgagattcat gcggttcatg
    24901 gcggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg tttgggcgcg tgtgtgatcg
    24961 tgattaatta cgatggcttg aatatggtgt atcaagaatt tgcaaatagt acaggaaatt
    25021 ctcgagtgaa agtaggcgat caagtgaaag ttggtcaagt aattgggata cgagatacag
    25081 cacacttgca tttaggattt actcgaatgg attggcgtca agcacaagga catgcattca
    25141 ttgatgatgg tacatggatc gatccgttac catttttgaa tagcagtaaa aaatagatca
    25201 ggagaaaaaa tatggaaaat aaaacgttaa aaatcgtctt aacagctgca attagcgtta
    25261 gtttaatcgg caatattgcg ttgttttggc agctgaaaga ggaacaagca gcaaatacct
    25321 ataaaattga agaagtggct aaaaacgcgt ctgagaagct caaaaaaaat tcctctgagg
    25381 cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga gaaaaaccaa gaaatcgatc
    25441 agctgaagca acaagatggc acaaaagcaa aagaggaagt aactgaagca caaagaaaga
    25501 cggcagaaga gttcggtcaa ttggccattg ataaatcatt aagcagaaat gaattaacgg
    25561 aaaaactaaa agatgtcgca acccaagaag tgatcgacaa attaagtcct acagacgatg
    25621 atcaccaaca tgatgattac ggaagttact cctttacatt aggtgacgtg caaacttacg
    25681 ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt atttgttgat tatacaatcg
    25741 gcaatccgca attcaaagat gtcaaaccac aaaaaataaa aggtggatta actgtcactg
    25801 aaaaacaagt agatggtcaa tggaaagtca tagactttag ttatttcact cgttaggagg
    25861 aagaaaatga gtaagaaaaa aggagaaaga caacgtttca aaaaacgaaa aagttatctt
    25921 agatggctgg gaatctttat gattctcggc tttttgattc ttgctagtcc gatttatacg
    25981 aatcagaagg tagatatacc agatgaggta agcacgaacg cctttttaaa cacgaaagag
    26041 ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat tagtttttga atttgcgatc
    26101 gatgatgaag atcaaagctt gttaaaagaa ctggctaatc ttaattatcg cgtagaagta
    26161 aagacagcta aaggggatta tcaagcaata cgagccaaag tgattaaggt gtctgatgat
    26221 tattttgcag tgaagctaac gcatgtacca gaagaatata tcgccatgcg tttaaccatt
    26281 attcctgaaa aaattgatcc aaaagtggat atgcaagaac cacaagatct gatttattac
    26341 gtgcacgaag ataaggttaa agatcaagtg aaagatgaag attatgaaca acacgcgatc
    26401 aattacaaag tgaaaggata caagaaagaa caaaaagcag ctcaaaaaca aatggaatct
    26461 ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga agctaaaaga tcaattgcct
    26521 tatcaagtcg cagatgatca ggaaaatact gaaaacaaga ttaatagcta ccagcaagaa
    26581 attgaaacat cgaaaacgca actgaaagat caagaagaga tcattcaaaa acttcaagaa
    26641 aaaattgatc gtatgacaaa agaaaagatt tagaaggaca ggtggagtga aaaaatgaag
    26701 cgaacaaact tggctgacaa agatactttc gctgagcaga aagtattgat taaagcctat
    26761 ctcaaagttg taaaagctaa agaaatatgt cgagaagtga atttatctaa cacaacaata
    26821 tctgagttga agaatggtcg aaagaattta gatactttaa aattcagatc atttgaagct
    26881 ctatatgtag ctgctaaaaa atattttaca gctgataaca aataaaaaac aggagtattt
    26941 tatgtgaaaa aagtatataa aaaatgctta tgaatcagct atatcacctt aaccatttgt
    27001 taatgagatg ggagaaaaca ttaaccacga ttctaacgca aacatcaagc acataaaaca
    27061 tttaacactc ctcctttctg gctgaagatt tcaaccaaac aggaagacag cgtatcaatt
    27121 atagcataca ctttttctta aattcatttt tcatatgaca aaatatttcc ttctaatcga
    27181 caaagttaga aggatttttt attgtctaaa tacacaaata accttaattt ttaaagagca
    27241 taaggaaacg tgatatcact ttctcacgaa aggtgttagg taggctgtgc ctaccacgtt
    27301 tctaagaaag ataaatgata tcaaaatttt ggatttgtgt gtagatttgt gggtaggatg
    27361 aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc aaaatcaaaa aatgtgtagg
    27421 gatatgtgta gctacacata tccctacaca taataaattt ggaggaaaat atggacttat
    27481 taattagaaa tattgaagaa aagtatatta ataaaattga caaacgttgt gaagaactta
    27541 cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct aaaagttttg atcgaaaatg
    27601 attttgatca cgctcttctg aattataaaa aagaccaatt tgatcggttg ttagaaaaat
    27661 ttgtggatat tcaaacttat aatacgaaaa ctttagaaga atatattgca acaaataatc
    27721 aattaattgg tttgttaatt gaataataaa aatagtattg tgaatttata aaaggaggaa
    27781 atcaattttg gcaaaagcaa caagaaaaca tgtatatctt tacgatatgc atatcgaagt
    27841 acttgatgaa ataatttcta ctcatgaaga tatatctaat tatagtgaag cagtcagaca
    27901 tttgtgtatg atgtatgatc caaaaatcga agaaaaaata aatccaattc aaattaaatt
    27961 gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg gtttcagagt ttgcggatct
    28021 tcatttgaaa gatacaggaa tactaattgg tacaaattca actgtttatc aagaagcaga
    28081 agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa aaatattctt ctaagttaaa
    28141 gaaaccagtg aatgaatctg ttcataccag agtagatcca tttgatccaa cgaagattct
    28201 aaaaagataa aagtagaagg agaacgtgat gaataaatcg cctgcagtta ttcttacttg
    28261 tcaatttacc ttgcccaata aaaaatcttt ttcaacatac attgattata tgacaagaga
    28321 aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa gaacaggaac tgcaacgaat
    28381 caagaatgct ttagaggatt tttatattcc tgaaggagaa actttttcgg aaagtaagac
    28441 ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac atggaatcaa
    28501 ttttgacaag ttagaggaac aagattttac caaatatctt tcttatatga cccgtcacta
    28561 tgcgttagaa caaaagaaag aactgacgca aagggaacaa aaagaatacc aacggttgaa
    28621 ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa gcttcaggaa atcttcctgg
    28681 agttttttct atggcttcag acgaagtgaa aggaagtgat ctgaaggaga tcaaacaaat
    28741 cttccaaaga ggacaacaaa aaggctcaat catttatcaa gatgttatct ctcacgacaa
    28801 ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca gataccttag atgaagaagg
    28861 tttgaaagca gctggaagaa aaatgatgga gacattattc gagaaagaac aattgaatga
    28921 aacaggttac tggatggcaa cgattcaccg taacactaag catatccata ttcattttgc
    28981 agttgttgaa aaagaaaata cgcgtaaacc gtatactgag attgaaaatg gtgtgagtta
    29041 tatcgtaccg aaggggaaga gaagacaagc aacattagac gcaatgaaaa ccaattatgt
    29101 acatgagtta gaacgtttct cttatgaaaa ggatcgaatg atcggtatgg agaaacgaaa
    29161 tttattgaca cgaaaaagtg atttgcgtaa caccttaaca aaagtagtaa aagaaccagc
    29221 acgttacgat cgacacgcca tgaacttatt aaatgaagta tataaaaccc tacctgaaaa
    29281 acgttcggaa tggaattatg gagatgaaaa tcgaaccaag ctttcaccat ttacaagggg
    29341 aaaacttgat gagttaacca aatatgtttt aacaaaagaa cctggatatc aagaatatat
    29401 acagatttca aaagcattga aagaagaagc caaaagcctt tatggggaaa gcaaaagaga
    29461 aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa aagcgcacag gaaatgcgat
    29521 tttaacagaa ctgaagagac aggatagtca aataaaacca ttaatagaaa gtgtcaatca
    29581 gatgcaatct atttacaatg aacgatcctt tcgaacactc aaacagttgc caacaaatga
    29641 atacaagtat agttatcaag aattcgtaaa gcgtaggaaa caattccaac aaagagttgt
    29701 cacgcagcga agcttgcgtc aattaaatcg aacaattaat gcacatgaag aaaaatacca
    29761 cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa atggaacaag aaagacaata
    29821 ggaggaagag aaatgaaaca atatttagat caatggaaag tcatagaagg atcactaaaa
    29881 gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag agcatttgtt tcagataagc
    29941 gaaatgttga gaaacgaaca gtttgatcca aatcaatttt tagttgttga gtacccagct
    30001 actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat attttattat tcaagaatac
    30061 gaaggtaagt tagcgcctta ttatactact tgggaaataa atgaagaggg aatcaataat
    30121 ttcccatgtg aaagcattga agagtctatt tctctcacgg aatgttaaat aaatagagac
    30181 gtaaaagagt cctgaacgtg attgtccagg actctttttc aatgaattgt tttccgccaa
    30241 ttcactgagt ggtgcgattc aattttatta tatcaaaaga aagtgagatt ccaaagtgaa
    30301 gggaggaaga agaaatgact cagacaacga ttggtgttgt ggttgaaaaa agcagggata
    30361 atctcgtttt tgtgacagaa attcaaacag gtagagcttt tgtagtcaca gacaaagctg
    30421 caaaagcgta tcaaaatggc aatattttaa cgttaaatct gactacgaag atgttcgttg
    30481 atgcagtaga aaattatcca tttgtttaga caaacgatat tttacaagga ggaaacggac
    30541 atggcagaag aaaaagaacc actagataat agtcggttgg gaaagtccaa aagaaaatta
    30601 gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc tatttgatca ccaaaaattg
    30661 acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt catggtttaa ccagcaagaa
    30721 aaaatagaaa ataaaataca aagtttgaga gaagagatca agcaacaaga aaaacaggta
    30781 gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
    30841 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
    30901 gaatcgactt tttcagccgc cacgatccga aaatatcaac ggaagttgga aacattagaa
    30961 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
    31021 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
    31081 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
    31141 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgcacca
    31201 actgattctc tgaaggagaa agaaaaagat catttctatc gagtagaatt caacgaaaat
    31261 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga attgatcgat
    31321 caacttaaaa cgttggataa ccaatacgct gcagaagcag gttattataa attctatttt
    31381 gaagaagtga agaacggaga agtagtagat cagatccgaa tggatctagg ggatggcgtc
    31441 aacagtaatc accccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
    31501 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
    31561 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagatcaa acaagaagtt
    31621 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc ttctgaagac
    31681 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
    31741 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
    31801 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
    31861 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
    31921 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta cacgattgaa
    31981 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
    32041 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
    32101 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
    32161 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
    32221 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
    32281 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
    32341 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
    32401 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagcggcg
    32461 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
    32521 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
    32581 caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc cgttggttca
    32641 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
    32701 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
    32761 agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
    32821 ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
    32881 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact atctgaaaaa
    32941 cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat gagtcttatt
    33001 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
    33061 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
    33121 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
    33181 tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc cactgatttt
    33241 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga aacagaaaag
    33301 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
    33361 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
    33421 gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
    33481 gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa
    33541 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
    33601 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
    33661 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
    33721 acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
    33781 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
    33841 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agtcgatcca
    33901 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
    33961 gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt
    34021 atggccatac acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac
    34081 aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga agccattaat
    34141 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg cgtggatggt
    34201 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca gattcttttc
    34261 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttatacct
    34321 gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca agaaatcaga
    34381 aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga gtgaaatcgt
    34441 catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt atgccgatag
    34501 cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat tgtttcctgg
    34561 tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc ccgatatgta
    34621 cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta cacattatca
    34681 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
    34741 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tcgcttggac
    34801 gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt ggatcaactc
    34861 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa aagagaccta
    34921 tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg ggatgaacgg
    34981 tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct tttccttagg
    35041 cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga ttagaaattt
    35101 caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg gcgcatttga
    35161 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
    35221 agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg aaaccgagaa
    35281 aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta aaatgaatca
    35341 gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt atgaagggaa
    35401 atatctaagc tatccacgaa cagacacccc ttatattacg gaaggggagt atgcgtattt
    35461 gttggatcat ttagatgaat acaagcattt tttaaaggcc gaatcaatcc caacccctat
    35521 tcatacgccg aatagtcggt atgtgaataa caagaaagtc caggaacatt acgcgatcat
    35581 tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac tgcagcaggc
    35641 catttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaaat atacctatga
    35701 agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg gaaaagtacc
    35761 gatagatcta ggttggaaga agttatttgg caaagagaac gacggaaaag agaaggaaga
    35821 agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc tacaagtcct
    35881 tgaaaaagaa accaagccac cacagcctta taccgaaggt acgttgatca cggcgatgaa
    35941 aacagctggg aaaaccgttg atagtgaaga agcgcaatcc atattgaaag aagtggaagg
    36001 aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa aggaatatat
    36061 caaggtagag aaaaataagc tcgtggtgac gaataaaggg attttgcttt gccaagccgt
    36121 ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc aaatgggaaa gctacttgtt
    36181 aaagatcggt gaacgaaaag gaacacaaac cacttttcta gccaatatcc aaaaatttgt
    36241 ctctcactta ttggaagtcg taccagggca aatccaatcc actgattttg gatcgacgtt
    36301 acaagaagtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag gaatttgtcc
    36361 aaaatgccag gaacaagaag tcttactcta tcaaaaggtg gcagcatgta cgtctgaagc
    36421 ctgtgatttc aaactatgga caacgattgc caaaaagaaa cttacagcga cacagctgaa
    36481 agagatcatt caaaatagcc gaacaagcca accagtgaaa gggttaaaag gtcagaaggg
    36541 ttcgtttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg aattttctga
    36601 aaagaaaaaa acgaacttca agaaaagaac acgacgcact acaaaataat ggagggttaa
    36661 tcttatgcgt aatacaacac acaaacaaca agaaatcgaa aatcaaaaag ccaaaattcg
    36721 ttcagaacag gaaaaaatgc gtgccagcaa acgaagactg cagcaattga agaaccagca
    36781 gcggattcaa aaagaaatcg aattagatcg ataaattcct ttcttgttaa tataaaacta
    36841 acatgttata gttgtgttag ttttatatta actttagggg aagagtaatg aaagacaaaa
    36901 tccaatgtat ttttgataaa cataatggca acctatcctt gaaggatgct agaagtgaag
    36961 gattatcgcc tatgacgtta gatcgattag ccgaaaagga taaaatagaa aaggtagcac
    37021 caggtttcta taccttaaag gatgcgttcg tggatgaatt gtatctcgct cagagtatct
    37081 attccagagg gattttttct catgagactg cgctcgatct ttttcaagta ggaacatata
    37141 tccctaaaaa gatcaatatg atgtttccta aaggatacaa tgtatcaaag gagaaattag
    37201 atcagtatgc cattcaaccg cattacacta ataaagaaaa ttttgacctg ggagtcacag
    37261 aaacagaaag cttttacggc aataaaattt tagtctatga tttagagcga acgttatgtg
    37321 acatgtggaa tccaagatac aaagcaagcg tagaggtaaa acaagaagca ttaaaagaat
    37381 atatgacaag taccagtaga aatacaaata agctaagaag atacatggat attttaaaat
    37441 caccaaaaga aatgactttg tatatgttgc cactttattg agaggagtaa agatgaatga
    37501 caccgacaca atttaaagag aaaacaagaa atttcgcagc agagaaacat ttaaacccac
    37561 aaattgtcca aagatattat atgatggaaa aattattaga atgtattgtt gaatcgcctt
    37621 atcatgatga ttttgtatta aaagggggat ttctgattgg atcaaaatac gggttagaaa
    37681 accgttcaac cgttgatatt gatacgactt atcgaaattc gaaactaaca aaagaaaagt
    37741 tagaaactgt tttttctgaa ttaacagcta atcctacaaa agatggaatt cgttttgaat
    37801 taaagggatt aacagagacg agggaagccg attattatcc aggttttcaa gcaaagttta
    37861 ttgcttatct tgaaaatgca cgtgtacctt ttaaattaga tattacttca ggtgattcta
    37921 ttattccagc agcattgatg tatgaacaca aattaatgtt tgaagataaa acgattcata
    37981 ttccagctta tcctacggaa caaattctag ctgaaaaatt acatgccacg tttagttttg
    38041 gaaaagataa ttcgcgcatg aaagattttt atgacctgta tatgattccg aaattagaac
    38101 gtatcgatga gaaaaatttg tataagagtg ttcaaggaac attcaaggaa agaaataatc
    38161 aatcggtgtt taagcatttg tatgatcagg aaatgcctca attggtgaaa gatgagggct
    38221 tgagagaaaa gtggaaaaga taccaacaag aaaactattt tgccaaagac atttctttcg
    38281 aatctacact aacttccgtt gattccctga tgcaatcaat tgtagagcaa gagaaggcag
    38341 aacgaaaaca aaattttaag agatcacaac aaatgaatca agaaatggag agataaagat
    38401 gatcaataat gtaactttag tagggcgatt aactaaagat ccagatttac gttataccgc
    38461 aagtggcaca gcagtggcga ctttttccct agccgttaat cgtaatttca cgaatcaaaa
    38521 cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag cagaaacgat
    38581 ggcgacatta gctaaaaaag gcagcttgat cggcgttgtc ggacgaattc aaacacggac
    38641 ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtggccg ataacttcca
    38701 actattagaa agtaaagcag cgacagaaag ccgtatgcac gctgatcaat cgtccagcag
    38761 tccaagccct accacctttg aacaacggga tacagcgacg cctaacaaca atggattgaa
    38821 cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg atctaccgtt
    38881 ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt tagacaacaa
    38941 aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggc tttcttttac
    39001 gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat ttacaatcaa
    39061 gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca aaatcaaaaa
    39121 gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag cagacagcaa
    39181 gactattata gcataattga aatatccaaa tgattggatt tttgctataa ttttagcgag
    39241 gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat aaaaacgcaa
    39301 ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg tgtagttgca
    39361 gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa tattgccgtc
    39421 acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt tagaaatccc
    39481 gatcatttta cgcgatcgat gggcatataa ataaaagaag agttgtagtc ctaaatgagg
    39541 attgcagctc tttttgtttt acatagaaac aggaaaagac ctttttagga agacacaagt
    39601 gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc accgccgttc gagaagaaag
    39661 gaagtgaacg ctgcacgttc actaagatat ggtgtcttgc aaagcaagac aaaatagaca
    39721 aaacttcctc tcaccaaaag gttcgttcct gttcgttatc tctttattga tttgccagac
    39781 cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga caagggtaaa tggcacgctg
    39841 cgcgcccttg tccttgtcgc ttttcgttga tttattctgg ccaacaaatc aataaagaaa
    39901 gaactaacga aaggaagtaa aacctttcct aagaggaggc agaatcaaac atgcgaaaga
    39961 aatttgatta ttggggtgtt cccttcagcg aattattccc aaactatcat gcaccacata
    40021 ctgttgaatg cgattgtgga gaaagagcga aatgtatcaa aagctatcgt ttgtatcaat
    40081 gtccaacatg cgggaaaaaa tataccttgt cttatggcga ttacattttg attgatgaga
    40141 aaggaaagaa acgctgatga aaacacaaca caaattattg actgtaaaag aagtcgttca
    40201 aattttggtg gacaaagaag tcatttctga aaaagtagca gaagctttac ttattccgcg
    40261 gtactggacg atagaaaccc ctttagtaga ggatgcaccg actatgctat ttgaagacct
    40321 agtgaagatt gcaggatatg aactgcatta tcaagaaaac atggaaactt atgaaatcga
    40381 atattggtta accaaattag agaactaaaa aggagaaaaa atatggctca aaattactat
    40441 gacgaatttg tcaaattacc tttggataaa atggcacaaa aaatggagga tatgacgttc
    40501 ctataccatg aaacacgtgt acccaaaaaa cattacaagg aaaaattgtc agtcgcagtg
    40561 gaagaaatga ttgaatcagg tgtcgaaatg aatctcattg cgacgtacta tcgaacactg
    40621 gaagaactga aaaaacaaaa tggaaaatgg tttttccaag ccttactatg cttggaagta
    40681 ggtgtcaaac caagcactat caaaccttcc gaatatcaag ccttagaact gacttatgca
    40741 aaattcgcac agattaagaa agctaaagca atggctagcg actggctaga tacttttgaa
    40801 agtatcgaaa aaaatggtgc attttatacg attgaatcgg aggagaaaga taatgaataa
    40861 ggtggtttat cagctaacaa cagaagatgt ttcgcaattt ttaacaagca aagaacactt
    40921 gaatgaagta atcagacgga ttcaaaaaga ctttagttgt atggaagaaa tgaagtactt
    40981 tattcaaaga gtggaagaac agttgtatat ggaaagcgaa acaagtcaaa cgaaggtcac
    41041 agtcagccat aatcatttag acgtgtataa acgtcgtcct gaaacggtaa aaaaaggaac
    41101 agagcttcta cgactagaaa cagaaaaata catcatgaat gtgtacctag aaaggagtga
    41161 gctggtcgaa aactttgtca gctacacgat ttgtaggaaa cactattact cagaatcaga
    41221 atattattca attttcgaga gcatggaatt aatggaagaa gaaatggaag agctaaacag
    41281 caaaaatgcg acggtagacg attattacct tgtatttact aggtttttga cgtgtgggac
    41341 ggaatctatc gtgttaatgg gaatggatta tgaactcgct gtacccgaag catttttgag
    41401 ggaatatgta gaagaagtag ataacccagg cgacgactgg tacacgactt taactgattt
    41461 tttagacacc tacacttacg acgatacaga agaaatccaa aatgaacttg accgtaaacg
    41521 aatgccatat caacgatttg aaaggaaaaa aggtaaataa ccatttatac cgaaaagtga
    41581 ggatgtgtga aataagaaca aaaaaccttg aaattaaggg aatggttctt atttcgcacc
    41641 aatcgctcca accgcccccg ccgcttaggt ggtgcgaaat aagcgggcaa agcccgataa
    41701 cctaagagaa agaaggagat ccatatgaat aaaatcattc ctctattgtt cgtactgttt
    41761 gttggcgaga tacttatgcg tcatgctgca gctgtacatt taaacttcgt agtattctta
    41821 ggaatactgg tattttgttt gatcttttgg cgatttgtat tagcccttgt gagtctacta
    41881 ttcaagatga ttgcagccat atttttcata gggttatttc gatgaaagga gagagtacta
    41941 atgccaattg caataggaaa taaacggtta cccgtgacat tagatgaaaa gagacaaaaa
    42001 gaattgcagc aactaaagca gaagtacgga aaaagtgaat ctaagattat gtgtattgcg
    42061 ttagatctat tgatcgcgca agaaaaagca ggatttgata taccagcact aaaaaagtga
    42121 cgtcactttt catttgaaga aacaaaagtg atagcactta attgttccta ctatttagaa
    42181 tattttctat gtccttttta ttccattttg tatcaataac tatgaaatct ttaaagtatc
    42241 cagaagagat tatttttaga tcaatttttt tcttacctga ttctaaagat tttgttttct
    42301 taaacatatt atgatttcct ttcgactatt ttcatataaa tgttaacata gtttatggat
    42361 tcatggaatg ttaatctaat ttaatagaaa aggataatta agtatatacg ttttttctta
    42421 tgtatgttat actatgtata tacagaaaga ggtgtctact atggaaatgt tacctataaa
    42481 aaaatgggga aatagtaatg gccttagatt gccaaagtat attatggaat atcttggaat
    42541 tcatacagaa gacaaagtga agataataca agaagaagta aacggtcaga agcggctaat
    42601 tatcgaagcg gctaatatag aaaatgaatt aacaattgaa cagttatttg aaaattacac
    42661 tgatgaaaga aatcatgtga cgattcaaaa tttaggtgaa gcagtaggta atgaaaaatg
    42721 gtgagaaaac ctagacaagg cgatattcta ttattgaata cagcacctcg atctggacac
    42781 gaacagacag gtaaaagacc ttatattgtt ttaagtcatg atattattgc ggattatagt
    42841 aacgttgtta cagttgctcc aatttctagt acaactagaa actatccgct atatgtaaat
    42901 ataaactcag aatatgaaat gaagacaaca ggaaaagtat tattagatca actaacgaca
    42961 attgattatg aagccagaga gtgtattttt ttggaaaaag cgcatgatag tttagttgag
    43021 gagttattga tgaaagttag aacagttttt caaaaagtaa gcaaataaaa tagaaacagc
    43081 agcatgatca actattcgtg attgaccatg ctgtcttttt ttattctata tacggtttgt
    43141 ctcgtaacat tgtagtcttt tgcaatctta gcgatcgcaa cacctttttt tagatcctca
    43201 acgatgtttt tataaacaag acgacgttgg ggatcttttg cattggcact gtatagctta
    43261 ggacgtcctt tataaacacc gttcgctttt gcaatcttaa tgccttgtgc ctgacgtcgt
    43321 ttagattcgg tacgttcttg ttcagcgatc atggccaaaa tttgaatgat caaatcttta
    43381 atgaatttat ccaggagagg attgccaatt gcttcggcca tgatcggtag acttgtgatg
    43441 atcagctgca catttttctt cttcaagtaa ttgactgaag cgatgatctc gtcgtaattt
    43501 cttccaagtc gatcaatagc ttccacaata aaaatatctt gatctcggac gaaatccagt
    43561 gcttcttgaa aaacaagtcg atgagtaatc gttgcgccag attgtttctc aatgaaaatt
    43621 ctctctgcac cgaatttttt catttcttca atctgccgtt gttcattttg atcgatggat
    43681 gagacacgta tgtatgcaac tctcaaaatt tttacctact ttctgcaact atttaaaaaa
    43741 caccctaatc atacacgaga aaaccagatt tttcaatgtc gcggtcgtgt ctagttaggg
    43801 tataccttat atagacaaaa actaatgatg gaggaaatag tatgtgtcac ttttcttttt
    43861 aaatggcaat attttttgta aataaatgtc aatcaagaaa atttaaacga ttatgacatt
    43921 aaaaatatat tttatgaatt ttctagtata taacttgatt tatctgttaa gatttattaa
    43981 gaaaacgttt tcttattttt gatttcgagg tgatagacaa tgaaaaataa gtttatttta
    44041 tctttaggta ttttaactat tggaattagt gtaactggaa ctacaattcc tacatttgct
    44101 gatagtacaa atgaaccaac tactgtagta gataataacc aagaattaac tcctttagat
    44161 aggaacggaa tagccattgg taacccaact aacgacttaa ctgttactga aaattattta
    44221 atagtttctc cattacttgc tcgagcagct tctaaaacga tacgagtgac aggaacgatg
    44281 atttttgata aagttggttg gccttctaga cctcctcaat cacggaattg gaaagaaaaa
    44341 agaaatggtt attggtacta tggaaaattg tatttacaaa gttataaatc agctggaaac
    44401 aattggattg cgacttattc aggaacctta actagaaagt agggattttt tatgccagaa
    44461 tttgtgaaca caccaaattt ttatcaagat agcgatattc taagtaaaat taatttaccg
    44521 aaagaagaat cagctttaca agttcagcga actttacagg agacacaaaa aattcaaaaa
    44581 tcaatttcta atctagaaga ttctgaagaa aaaagaaaaa aaagagacca aaaaacatat
    44641 caaaaagaaa aataatgatt ttaaaaaaag agtgtctagt cgacattctt tttttctaat
    44701 tgaaagtgtt cataaaatca cccatttctg gacactttct ttttttgctt tatttttcaa
    44761 aaaaacaatt catttcatgt tcataaaccc ataacaatat ctatgttcag ttaaactata
    44821 tggataaagt tatgttacaa taaaattaat gaaggagtga tttttttgaa aataggatac
    44881 gcccgggtat ctactggttt acaaaatttg gatttacaag aagacagcct aaataaattt
    44941 ggttgtgaaa aaatttttac tgatcatatg agtggggcaa aaagtaatcg accaggatta
    45001 gaaatggcca ttgattttgt tcgctctggc gatacacttg tcgtttggcg cttagatcgt
    45061 ttaggtagaa atatggaaga tttgatttca atcgtcaatc gactgaatga gcgtggtgtg
    45121 agttttcata gtattcaaga aaatattacg atggacaaat caagttctac aggacagctc
    45181 atgtttcatt tgtttgcggc atttgcagaa tttgagcgta atttgatcct ggaacgatcc
    45241 gcagcaggac gagcagctgc tcgtgcaaga ggacggtttg gtggtcgtcc tgaaaaattg
    45301 tctgaacaag atttggaatt actgaagact ttagtggata gcggtacacc tattaagact
    45361 attgcggaac ggtggaacgt ctcaagaacc accatttatc gttatttggc aaaaatggag
    45421 gagaaaaatg gaaagacaaa ttctcattag tttttgtcat ttaatgctga aaatttttgc
    45481 catttatcta gaaaagtgac aatagtatga gaattttttg taagtgtcta ctgataactt
    45541 attctcaaat gttgtaagga ttttattcat ttgttaatat ta
//
LOCUS       DAYYGY010000012        44880 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000012 DAYYGY010000000
VERSION     DAYYGY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.012"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHA52_000740"
     CDS             212..604
                     /locus_tag="ACHA52_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8939489.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHA52_000741"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHA52_000741"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8939490.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHA52_000742"
     CDS             2698..2943
                     /locus_tag="ACHA52_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939491.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHA52_000743"
     CDS             3165..4775
                     /locus_tag="ACHA52_000743"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8939492.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHA52_000744"
     CDS             complement(4752..4964)
                     /locus_tag="ACHA52_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939493.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHA52_000745"
     CDS             5067..5936
                     /locus_tag="ACHA52_000745"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8939494.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHA52_000746"
     CDS             6101..7372
                     /locus_tag="ACHA52_000746"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8939495.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHA52_000747"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHA52_000747"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8939496.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHA52_000748"
     CDS             8763..9026
                     /locus_tag="ACHA52_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8939497.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHA52_000749"
     CDS             9471..9920
                     /locus_tag="ACHA52_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8939498.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHA52_000750"
     CDS             9977..11395
                     /locus_tag="ACHA52_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8939499.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHA52_000751"
     CDS             11395..12255
                     /locus_tag="ACHA52_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8939500.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHA52_000752"
     CDS             12429..13202
                     /locus_tag="ACHA52_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8939501.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHA52_000753"
     CDS             13349..13714
                     /locus_tag="ACHA52_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8939502.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHA52_000754"
     CDS             complement(13862..15070)
                     /locus_tag="ACHA52_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8939503.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHA52_000755"
     CDS             complement(15089..15367)
                     /locus_tag="ACHA52_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939504.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHA52_000756"
     CDS             15560..16216
                     /locus_tag="ACHA52_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8939505.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHA52_000757"
     CDS             complement(16658..17455)
                     /locus_tag="ACHA52_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8939506.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHA52_000758"
     CDS             complement(17452..18636)
                     /locus_tag="ACHA52_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8939507.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHA52_000759"
     CDS             complement(18661..19908)
                     /locus_tag="ACHA52_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8939508.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA52_000760"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA52_000760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8939509.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHA52_000761"
     CDS             21888..22202
                     /locus_tag="ACHA52_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8939510.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHA52_000762"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHA52_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8939511.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA52_000763"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA52_000763"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8939512.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHA52_000764"
     CDS             23546..23875
                     /locus_tag="ACHA52_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8939513.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHA52_000765"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHA52_000765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8939514.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHA52_000766"
     CDS             24863..25687
                     /locus_tag="ACHA52_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8939515.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLLEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHA52_000767"
     CDS             25680..26027
                     /locus_tag="ACHA52_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8939516.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA52_000768"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA52_000768"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8939517.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA52_000769"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA52_000769"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8939518.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA52_000770"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA52_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8939519.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA52_000771"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA52_000771"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8939520.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHA52_000772"
     CDS             31684..32451
                     /locus_tag="ACHA52_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939521.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHA52_000773"
     CDS             32441..34483
                     /locus_tag="ACHA52_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939522.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACHA52_000774"
     CDS             34764..34913
                     /locus_tag="ACHA52_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8939523.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACHA52_000775"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACHA52_000775"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8939524.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACHA52_000776"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACHA52_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8939525.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACHA52_000777"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACHA52_000777"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8939526.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACHA52_000778"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACHA52_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8939527.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACHA52_000779"
     CDS             39330..40412
                     /locus_tag="ACHA52_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8939528.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACHA52_000780"
     CDS             40412..41215
                     /locus_tag="ACHA52_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8939529.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACHA52_000781"
     CDS             41285..42025
                     /locus_tag="ACHA52_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8939530.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACHA52_000782"
     CDS             42036..43040
                     /locus_tag="ACHA52_000782"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8939531.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44878)
                     /locus_tag="ACHA52_000783"
     CDS             complement(43085..44878)
                     /locus_tag="ACHA52_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939532.1"
                     /translation="MKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYT
                     KDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKIL
                     YQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLIS
                     FIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSE
                     DILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVL
                     LYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHC
                     LIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLL
                     EQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCS
                     FCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETH
                     AIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDV
                     ADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14425 a         7831 c         9343 g        13281 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cttgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttatttt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
//
LOCUS       DAYYGY010000013        41707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000013 DAYYGY010000000
VERSION     DAYYGY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.013"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA52_000784"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA52_000784"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8939533.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA52_000785"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA52_000785"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8939534.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA52_000786"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA52_000786"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8939535.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHA52_000787"
     CDS             2179..2499
                     /locus_tag="ACHA52_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8939536.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="ACHA52_000788"
     CDS             complement(2611..3480)
                     /locus_tag="ACHA52_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8939537.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHA52_000789"
     CDS             complement(3576..4880)
                     /locus_tag="ACHA52_000789"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8939538.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHA52_000790"
     CDS             complement(4877..5386)
                     /locus_tag="ACHA52_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939539.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHA52_000791"
     CDS             complement(5561..7696)
                     /locus_tag="ACHA52_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8939540.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHA52_000792"
     CDS             complement(7779..9434)
                     /locus_tag="ACHA52_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8939541.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHA52_000793"
     CDS             complement(9436..9750)
                     /locus_tag="ACHA52_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8939542.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHA52_000794"
     CDS             9920..10180
                     /locus_tag="ACHA52_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8939543.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHA52_000795"
     CDS             10194..10508
                     /locus_tag="ACHA52_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8939544.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHA52_000796"
     CDS             complement(11238..11792)
                     /locus_tag="ACHA52_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8939545.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHA52_000797"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHA52_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHA52_000798"
     CDS             complement(14153..14869)
                     /locus_tag="ACHA52_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8939546.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA52_000799"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA52_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8939547.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA52_000800"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA52_000800"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8939548.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHA52_000801"
     CDS             complement(18339..19799)
                     /locus_tag="ACHA52_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939549.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHA52_000802"
     CDS             complement(19789..21084)
                     /locus_tag="ACHA52_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8939550.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHA52_000803"
     CDS             complement(21416..22093)
                     /locus_tag="ACHA52_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8939551.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHA52_000804"
     CDS             complement(22123..23214)
                     /locus_tag="ACHA52_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8939552.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHA52_000805"
     CDS             complement(23315..23749)
                     /locus_tag="ACHA52_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8939553.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHA52_000806"
     CDS             complement(24333..25790)
                     /locus_tag="ACHA52_000806"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8939554.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHA52_000807"
     CDS             complement(25787..27682)
                     /locus_tag="ACHA52_000807"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8939555.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHA52_000808"
     CDS             complement(27740..29515)
                     /locus_tag="ACHA52_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8939556.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHA52_000809"
     CDS             complement(29565..31061)
                     /locus_tag="ACHA52_000809"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8939557.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHA52_000810"
     CDS             complement(31255..32877)
                     /locus_tag="ACHA52_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8939558.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHA52_000811"
     CDS             complement(33205..34518)
                     /locus_tag="ACHA52_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8939559.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACHA52_000812"
     CDS             complement(34763..34987)
                     /locus_tag="ACHA52_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939560.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACHA52_000813"
     CDS             complement(35007..35429)
                     /locus_tag="ACHA52_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8939561.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACHA52_000814"
     CDS             complement(35571..36281)
                     /locus_tag="ACHA52_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8939562.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACHA52_000815"
     CDS             complement(36299..36796)
                     /locus_tag="ACHA52_000815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8939563.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACHA52_000816"
     CDS             complement(36833..37285)
                     /locus_tag="ACHA52_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8939564.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACHA52_000817"
     CDS             complement(37420..38025)
                     /locus_tag="ACHA52_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8939565.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACHA52_000818"
     CDS             complement(38475..39095)
                     /locus_tag="ACHA52_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8939566.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACHA52_000819"
     CDS             complement(39097..39966)
                     /locus_tag="ACHA52_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8939567.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACHA52_000820"
     CDS             complement(39989..40396)
                     /locus_tag="ACHA52_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8939568.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="ACHA52_000821"
     CDS             complement(40415..41704)
                     /locus_tag="ACHA52_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8939569.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7022 g        13943 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DAYYGY010000014        37502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000014 DAYYGY010000000
VERSION     DAYYGY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.014"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACHA52_000822"
     CDS             256..531
                     /locus_tag="ACHA52_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939570.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2622
                     /locus_tag="ACHA52_000823"
     CDS             544..2622
                     /locus_tag="ACHA52_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8939571.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2785..4668
                     /locus_tag="ACHA52_000824"
     CDS             2785..4668
                     /locus_tag="ACHA52_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8939572.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4680..5627
                     /locus_tag="ACHA52_000825"
     CDS             4680..5627
                     /locus_tag="ACHA52_000825"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8939573.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="ACHA52_000826"
     CDS             5647..6174
                     /locus_tag="ACHA52_000826"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8939574.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="ACHA52_000827"
     CDS             complement(6237..6890)
                     /locus_tag="ACHA52_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8939575.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="ACHA52_000828"
     CDS             7022..7864
                     /locus_tag="ACHA52_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8939576.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="ACHA52_000829"
     CDS             8022..8918
                     /locus_tag="ACHA52_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8939577.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="ACHA52_000830"
     CDS             8921..9634
                     /locus_tag="ACHA52_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8939578.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="ACHA52_000831"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="ACHA52_000831"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8939579.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="ACHA52_000832"
     CDS             10169..10396
                     /locus_tag="ACHA52_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8939580.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="ACHA52_000833"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="ACHA52_000833"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8939581.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11724)
                     /locus_tag="ACHA52_000834"
     CDS             complement(11182..11724)
                     /locus_tag="ACHA52_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8939582.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="ACHA52_000835"
     CDS             complement(11961..12359)
                     /locus_tag="ACHA52_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8939583.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="ACHA52_000836"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="ACHA52_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8939584.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="ACHA52_000837"
     CDS             13472..14239
                     /locus_tag="ACHA52_000837"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8939585.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="ACHA52_000838"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="ACHA52_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8939586.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="ACHA52_000839"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="ACHA52_000839"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8939587.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="ACHA52_000840"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="ACHA52_000840"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8939588.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18895..19800
                     /gene="xerC"
                     /locus_tag="ACHA52_000841"
     CDS             18895..19800
                     /gene="xerC"
                     /locus_tag="ACHA52_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8939589.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19837..20385
                     /gene="hslV"
                     /locus_tag="ACHA52_000842"
     CDS             19837..20385
                     /gene="hslV"
                     /locus_tag="ACHA52_000842"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8939590.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20399..21799
                     /gene="hslU"
                     /locus_tag="ACHA52_000843"
     CDS             20399..21799
                     /gene="hslU"
                     /locus_tag="ACHA52_000843"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8939591.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21817..22608
                     /gene="codY"
                     /locus_tag="ACHA52_000844"
     CDS             21817..22608
                     /gene="codY"
                     /locus_tag="ACHA52_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8939592.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22690..23565
                     /locus_tag="ACHA52_000845"
     CDS             22690..23565
                     /locus_tag="ACHA52_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8939593.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23697..24335)
                     /gene="plsY"
                     /locus_tag="ACHA52_000846"
     CDS             complement(23697..24335)
                     /gene="plsY"
                     /locus_tag="ACHA52_000846"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8939594.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24462..24896
                     /locus_tag="ACHA52_000847"
     CDS             24462..24896
                     /locus_tag="ACHA52_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8939595.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25062..27107
                     /gene="parE"
                     /locus_tag="ACHA52_000848"
     CDS             25062..27107
                     /gene="parE"
                     /locus_tag="ACHA52_000848"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8939596.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27119..29569
                     /gene="parC"
                     /locus_tag="ACHA52_000849"
     CDS             27119..29569
                     /gene="parC"
                     /locus_tag="ACHA52_000849"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8939597.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29846..32077
                     /gene="pflB"
                     /locus_tag="ACHA52_000850"
     CDS             29846..32077
                     /gene="pflB"
                     /locus_tag="ACHA52_000850"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8939598.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32321..33082
                     /gene="pflA"
                     /locus_tag="ACHA52_000851"
     CDS             32321..33082
                     /gene="pflA"
                     /locus_tag="ACHA52_000851"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8939599.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33167..34093
                     /locus_tag="ACHA52_000852"
     CDS             33167..34093
                     /locus_tag="ACHA52_000852"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8939600.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34275..35168
                     /locus_tag="ACHA52_000853"
     CDS             34275..35168
                     /locus_tag="ACHA52_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8939601.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35302..35427
                     /locus_tag="ACHA52_000854"
     CDS             35302..35427
                     /locus_tag="ACHA52_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8939602.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35690..37237)
                     /locus_tag="ACHA52_000855"
     CDS             complement(35690..37237)
                     /locus_tag="ACHA52_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8939603.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(37339..>37502)
                     /gene="tnpB"
                     /locus_tag="ACHA52_000856"
     CDS             complement(37339..>37502)
                     /gene="tnpB"
                     /locus_tag="ACHA52_000856"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8939604.1"
                     /translation="FLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLSIDQPRA
                     ILPGKKGVF"
BASE COUNT        12959 a         6516 c         7839 g        10188 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagactatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcattttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacaccgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag
    18781 gaattgcgcc aattgtgaac aaacttagaa atattggtta aattctgaca atttatttgt
    18841 ctttttataa acgctgtgct aaattattct ctgggacaaa aggaaggtga gaaaatgaca
    18901 gaagccacgt gggaacaaaa atttttgcgt tatttgattg tcgaaagagg atattcagaa
    18961 aaaacgaaag aagcatatga agaagacatt ctccatttca aacatttttt ggaggaatct
    19021 ggagacgctg atctgctgaa ggcggaatat tttacggttc gtgtctattt gagtgcatta
    19081 gctgatcatc gtcctagcta tagcagaaat tctatcagtc gcaagatagc cagcctacgt
    19141 tcgttttatc agtatcttct caaagaagaa gtgataaaag aaaatccatt ttcttacgtt
    19201 catctcaaaa agaaaaattt gagactgcca cgtttttttt atgaaaatga aatgcaagca
    19261 ctttttgaca gcgtagcagg agatagtcct ttggatttga ggaaccgcgc tttactggaa
    19321 gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca gtttgtcggt atcagacatt
    19381 gattttgatt cagaggtcat gttgatccat ggtaaaggga acaaagagag atatgctcct
    19441 ctaggttctt ttgctcaaga tgctctgcaa gaatatttca gggaaggccg ccaggtattg
    19501 atgacaaaat atcacgaaga gcatgatatc gtttttgtga atcatcatgg aaaaccaatc
    19561 acgcctactg gaattgaata tgtcttgaat caaatgatca aaaaaagcag tttggatagt
    19621 gatatccatc ctcacatgct gcgccataca tttgctactc atttattaaa taatggggca
    19681 gatttgcgga cagtccaaga attgctagga catgccaatt tatcgactac ccagatctat
    19741 gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa
    19801 aaataaggaa aagaacaaat ttaggaggaa acaaacatgg tcgaatcacc atttcattca
    19861 accactatct gtgccgtcga aaaagacggg aaatttgcta tggctggaga tggccaagta
    19921 acaatgggtg aatctgttgt catgaaaggc actgcaaaga aagtacgacg tatctacaat
    19981 ggagaagttg ttgtaggttt cgccggaagt gttgcagatg cgtttacttt agaagaaaaa
    20041 tttgaaggca aactaaacga atataacggg aatctacaac gcgcagctgt cgaattagct
    20101 caagaatggc ggacacagca atctatgcaa aaacttgaag cgatgctgat cgtaatgaat
    20161 gataaagaaa tgcttcttgt ttcaggtaca ggagaagtta tcacaccaga tgatggtatc
    20221 ttagctatcg gatcaggagg gaattttgcg ttggctgcag cccgagcaat gaaaaactat
    20281 gctcaaaacg acatgagtgc gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat
    20341 atctgtatct ttactaatca taatattatc gtagaagaac tgtaagaggt gagtagatat
    20401 gtatgaatta aaacaaacac caagacaaat cgtaagtgaa ttagatgaat atatcgtagg
    20461 acagcacgca gcaaaaaaat ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca
    20521 gttagatgaa aaaatgcaac aagatattac accaaaaaat atgctgatga tcggacctac
    20581 aggggtcggt aaaacagaaa tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt
    20641 caaagtcgaa gcaacaaaat ttactgaagt cggttatgtc ggaagagatg tagaatcaat
    20701 ggttcgggat ttagtagaaa atgcgatcca aatcgtagaa aaagagcagt acagccgtgt
    20761 atacacaagt gctaaaaaaa atgcagaaaa acgtttggta aaaatcttgg tacctggtat
    20821 caaaaaagaa cagaaacaat ccactaatca atttgaacaa atgatgaatc tttttaatgc
    20881 tagtcagcaa aataccgctg aaccgcaaga agaagtgaca gaagatattc gaatcaatcg
    20941 ccgtacagtc ttggaacaac tacaaaaggg tttactggat agtcgtgaag taaccattga
    21001 agttgaagac caaaagaaac ctgcaccaat gatgaacaat ggattggagc aaatggggat
    21061 cgacctaaat gaaactctag gtgctttgac accgaagaaa aaagtggaac ggacggtaac
    21121 agtcaaagaa gctttagagt tattgatcaa ggaagaatca gcaaaattag tcaaggatgc
    21181 tgatatccat agtgaggcaa tcaaattagc ggaagaaagt ggaattattt ttatcgatga
    21241 aatcgataag atcacttcta aatcacagca atctggagaa gtatccagag aaggcgtcca
    21301 aagagacata ttgccaattg tcgaaggttc acaagtaaac acaaaatacg ggatcatcca
    21361 aacagatcac gtcctattca ttgcttcagg agctttccat ttgtctaaac caagtgatct
    21421 aattccagaa cttcaaggac gcttcccgat acgggtagaa ttagatgatt tgacagctga
    21481 agattttgtg aagattctga ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt
    21541 gatcggtaca gaaaatgtgt cagttacgtt tacaaaagaa gcaattgaac ggatcgctca
    21601 aatcgctttc gatgtcaacc gtgacacaga taatatcggc gcacgcagac tgcataccat
    21661 tttggaaaaa ctactagaag atctattatt tgaagcacca gatatgcaaa tgggagaaat
    21721 aacgatcaca gaagcatatg tggatgacaa acttggctca attgcggcaa atgaagattt
    21781 aagtcgctat attttataaa aaatcggagg acgacaatga cagacttgct aacgaaaacc
    21841 cgcaagataa acacactttt gcagcaaaag aatacttttg acatgcaagc agatttgcct
    21901 tacgataaaa tggctgtcac actaggtggt attcttgaca gcaacaccta tatcatagga
    21961 agtgacggag tattactagg ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa
    22021 acaatgtttg ttgagaaaca atttcctgaa tcttatactg atacagtggc acagttgatg
    22081 aaaactgaag ctaatatccc aattactagt gatattactg catttccggt agagtcaaga
    22141 gagaaatatc catttggtct gacgactgtc gttccgatct ttggagctgg cgaacgtttg
    22201 ggtacgatta tcttatctcg tatggaacaa tcgtttgcag atgacgatct agtcttagca
    22261 gaatatggag ctacggttgt cggtatgcag atcctttatc agaagtctcg aagcatcgaa
    22321 gcagatgtac ggagtgcaac ggctgtccaa atggcaatca acacattatc ttatagcgaa
    22381 ttgaaggctg tacaagctat ctttgaagca ttggatggag atgaaggtcg tctgactgct
    22441 tctaatattg ccgatagtat cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa
    22501 ttggaatcag ctggaatcat tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa
    22561 gtattgaatt cccgttttaa agacgagtta gcaaaacaca gttattagaa gtaaggggac
    22621 tggggcagat agaaatgttt gttccagcct tcccatttta aaaagtaagt cgaagttgga
    22681 ggaatggcaa tgactgttat tattcaaaat gaccagctga tcgccgaaat cgctgaacac
    22741 ggtgcggaat tgatcagtct gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt
    22801 gatccagcgt attggggcag acatgcaccg gttttattcc cttttgttgg acgtttaaaa
    22861 gatgatcagt atgtttatca agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg
    22921 gatatggatt ttgaagtcat tgaacatgga caagaaatgg ctagtttcat cttaaggagt
    22981 acgccggaga cattagaaaa gtatccattt gattttgaat tagtgatcag ctatgaatta
    23041 ggaggagacg gcatcacggt tcattatcaa gtggcaaata ccggtgaaaa agaaatgttc
    23101 ttttctatcg gtggacatcc tgctttcaat gtacctcttg aagaaggatt gtcatttgaa
    23161 gatttctatc tggcattttc tccattgaaa tcaagattac gcctaccatt gaaaggccca
    23221 ttcatcgata tggatcaaaa gacgttaggt cagacaaata cgaatctggc tttgaatcgt
    23281 gaactttttg agcaagatgc cttggttttt gaaacaaggg gtctaaatgc gtacgccatt
    23341 cgaagtgaaa aatcttccca tagcgtaact ttaagctata aagatatgcc ttatgtaggg
    23401 atctggtctc cttatccaaa agaagcgcct ttcgtttgta tcgaaccgtg gtgcggaatc
    23461 gctgacacgg tgaacagtag tggaaacttg atggaaaaag tagggatcaa tcaactaggt
    23521 gcccatgaaa tcttcaagac aaaatattcc atcacagtga agtaagaggt tgtgaccgag
    23581 aagttagcag ggaacaccgt ttaccaaggt gtgtaaaaaa gctatcctgt aagtaataag
    23641 ggagtgtgac atcgatttgt cacactcccg ttttatctct ttggaatgaa aaagtatcag
    23701 gattttttct tgctgctatt caaaccaaac gggattcgac tttcagtatg gtttctgata
    23761 cgcttgatat tgtctttatg ccgataaaag ataaaggaag aaactaaaaa ggcaatgatc
    23821 gtcagtaacc aattgggctt agccaaaatc acaggagcta cataaggaag gacgatcgta
    23881 gataaagtga tcaatactgc actgatcata ctgctcaaac tcaccatact tgtacaaaat
    23941 aaactaacga taaagatgat acaagaataa acaaaaaata caggattaaa tcctaaaagc
    24001 attcctgcac ttgtcgcaac agcttttcct cctttgaacc cagcaaaaat cggaaatgta
    24061 tggccaagaa tggcgcatac cccaaaccaa agagggttaa tcccagaaag gtggaagatc
    24121 atcggcaaac aagtagccaa tgtacctttt aaaatatcca taagcaggac aacaatcccg
    24181 gctgttttgc ctaatacacg aaatgtattg gtcgttccag tattaccgct cccgtgttgt
    24241 cggatatctt ttttgaaaaa caatttgcca atccagacac cggaaggaat agaacctaaa
    24301 agatatgcta aaataagtaa taagatcaat ttcatttttt accctctttc aagttgaaaa
    24361 ctatccttta ttttagcatg aataggtaac aggaacaatt gaaaaaatga aagatacaag
    24421 tataatagat ggaataaaaa gaaaagcaag gggtggcaga aatgtctgta aaaaatccaa
    24481 cacaagaaac gattttcaac tatttggaag aagcaaaaaa tattgccgtc gtaggcttga
    24541 gcaataaacc agaacgaaca agttacatgg ttgcacaggt attgcaagac aatggatata
    24601 aaatcatacc agtaaatcca ttattggctg gtgaagaaat tctaggcgag aaagtctatg
    24661 cgaatttgac agaaatcgat gatccaatcg atatcgtaga tgtatttaga cggagtgaat
    24721 ttttacctga aatagcaggt gattttttga aaactgatgc taaagtgttc tgggcacaat
    24781 tagggattga aaacaaagaa gcagctgaga tgctaagaaa agcaggacgt acagatatcg
    24841 taatggatcg ttgtattaag attgaacttg gtaagttgaa agaaaaccaa aaataaaaat
    24901 gaccacaaag aagagatgat cgatagaggg gaatgtcgaa aaagcggtta ggagataatt
    24961 aaaattgatt tctcgtctac cctctttttt ttcacgacct tttctagtat gataatcgtg
    25021 gtgcggtttt gccgcaaagt attattgaag gagcgtttgc cttggcaaaa aaagtaaaca
    25081 acgaatacaa tgactcgtcc atccaagtat tggaaggatt agaggcagtt agaaaaagac
    25141 cagggatgta cattggatcc actgacagcc gaggcttgca tcatttagtc tatgaaatcg
    25201 tcgataatgc agttgacgaa gcattatcag gttatggcag tgagattgat gtgaccatcc
    25261 atgaagacaa tagtataact gttgcagata gcggacgtgg gatgcctgta ggaatgcatg
    25321 cttccggaat accaacagtt gaagtgattt ttactgtgct gcatgctggc ggaaagtttg
    25381 gtcaaggagg gtacaaaact tctggtggac tgcacggtgt aggagccagt gtggtaaatg
    25441 ccttatcgaa atggctaatt gtaacgatcg ttagagatgg cgtagagtat cagcagaaat
    25501 ttaaaaacgg cggtaaacct gacggtactt tgaaaaagat aggaaaaaca aaaaaagcaa
    25561 acgggacgac tgtccatttt ttaccagatg atacaatatt cagtacaacg aaattttcat
    25621 atgaaatttt agccgaacgg ttaagagaat ctgcctttct tctaaaaggc gttaaaatct
    25681 ctctttctga tcttcgtgga gaagaaccag tcaaggaaat ttttcattac gaagaaggaa
    25741 tcaaagaatt tgttgattat ctgaatgaag aaaaagatac attgacacca gttgtttatt
    25801 tttcaggaga aaaagaaggt atcgaagtag aggtcgctta tcaatacaat gacggttatt
    25861 cagaaaatgt tttatctttt gtcaataacg ttcggacaaa agatggggga acccatgaag
    25921 ctggtatgaa agcggcgatg acgaaatctt ataatgaata tgcgagaaaa gttggtttgc
    25981 tgaaagaacg ggacaagaat ctggaaggta gtgatttcag agaagggtta gctgctgtac
    26041 tttctatccg tgtacctgaa aatcttctgc aatttgaagg gcaaacaaaa gaaaaacttg
    26101 gtacgccagt tgctcgtacc gtagtagata acgtcatcag tgaacaaatg ggcttttatt
    26161 tgcaggaaaa tagcgaaatg agccagatgt tggtccgtaa agcaatcaaa gccagagaag
    26221 cgagagaagc agcacgtaaa gccagagaag aaagccgaaa tgggaagaag cgtaaaaaag
    26281 gcgaatctct tctttcagga aaattgacac ccgcacaatc ccgaaaccct aagaaaaatg
    26341 aattgtattt agtcgaaggg gattctgctg gcggatctgc taaacaagga agagacagaa
    26401 agttccaagc aattctacct ttacgaggaa aagtcatcaa taccgaaaaa gctaaaatgc
    26461 aagatatctt gaaaaatgaa gaaatcaata cgatgatcta tacgattggt gcaggagtcg
    26521 gtcctgagtt ttctatcgaa gactgtaatt acgacaaagt aatcatcatg accgatgcgg
    26581 atacagacgg cgcccatatt caagtattac tgcttacttt cttttatcgt tatatgaaac
    26641 cgctgattga agctgggaaa gtatatatcg cgttaccgcc tttatataaa gtatccaaag
    26701 gacaaggaaa gaagcaagtc attgaatatg cgtggacaga tgacgaattg gcagcaatga
    26761 ttaaaaaagt cggcaaagga tatatgctgc agcgatataa aggacttgga gagatgaatg
    26821 cggaacaatt gtgggaaaca acgatggatc cgacttcccg tacgttgatc cgcgtccgta
    26881 tcgatgatgc tgcgcaagct gaacgtcgag taaccacact gatgggtgac aaagtagaac
    26941 ctcgaagaaa atggatcgaa aatcacgtgc aatttacgct cgaagaagat ggcagtatct
    27001 tagataaaaa agaggatact gaaatctctc cttcagtttc taatgattta ttagatgaag
    27061 aacgagcaga taaaaatgaa aacaatcagt tatttgaagt agaataggag gtcacctgat
    27121 ggaaaatcga caagaagtac aagaattaac tttagaagaa gtgatgggtg accgcttcgg
    27181 acgttattcc aaatatatta tccaagaacg tgcactgcct gatatccgtg atggattgaa
    27241 gccagtgcaa cgccggatat tatttgccat gaacaaagat ggcaatacgt atgacaaagg
    27301 attccgtaaa tcagcaaaat ctgtcggaaa tatcatgggg aattatcatc ctcatggtga
    27361 catcagtatt tatgaagcga tggtccgcat gagccaagac tggaagttgc gggaagtact
    27421 gatcgaaatg cacgggaata acgggagtat ggacggtgat ccacctgctg cgatgcgtta
    27481 tactgaagcc agattatcca agttaagtgg agagatgcta gcagatatcg agaaagaaac
    27541 agtcgatctc gtgtggaatt ttgatgatac agaaaaagaa ccaacagtcc ttccagctcg
    27601 atacccaaac cttctcgtca atggatcgac aggtatatct gctggatatg caacagaaat
    27661 cccgactcat aacttagctg aagtgatcga tggcacaatc tatatgatcg atcatccaca
    27721 agcgagcctt gaaaaattga tggaatatat tcctggacct gactttccaa caggagggat
    27781 cttgcaagga aaagatgaaa tcaaaaaagc ttatgaaaca ggacgaggaa aagttattct
    27841 tcgttcgaaa acaaagatag aggcaatgaa gggtaataaa cagcaaatcg tgatcacaga
    27901 aatcccatat gaagtaaata aagcgacctt ggtgaaaaaa atggatgaga tccgtttgaa
    27961 taagaaaatc gatggaatag ctgaagtacg agatgaaagt gatagaacag gtctgcaaat
    28021 tgtcgtcgaa ttgaaaaaag acgccaatgc tcaaggaatt ttgaattatt tgttcaaaaa
    28081 cactgagcta cagatcaatt acaatttcaa tatggtcgct atcgaccata tgactccgca
    28141 tcaagttgga ctgaaagaca ttttaagaag ttatatcgag catcgaaagc aagtcataac
    28201 aaaacgtagt caattcgatt tagccaaagc tcaaaaaaga cagcatatcg tagaaggatt
    28261 gatgaaagct ttatcgattc tggatgaagt aatcgcaacg attcgtgaaa gtaaagacaa
    28321 aaaagacgcc aaaaagaatt tagtcgacgt ctttcaattt acagaagaac aagcagaagc
    28381 aattgtcaca ttgcaattat accgtttgac gaacacggat atcacagagc ttcaaaagga
    28441 atccgaaagc ttgattgcgc aaatcacaga gttgaataaa atcctttcaa atgacaaaga
    28501 gcttttctct gttatgaaaa aagaattgcg agaagtgaaa aaaaattatt cttccgctcg
    28561 tctaacgaca atcgaagacg aaatcgaaga aatcaaaatt gatacgcaag tcttagtggc
    28621 acaagaagac gtaattgtca gtgtgacgcg agaaggctat gtgaagcgaa caagccttcg
    28681 ttcttatagt gcttccaaac cagaagaaat cgggatgcga gaaggcgact acttgctata
    28741 ctcaggtgaa ttgagtacat tggaccatgt tcttctgatc acaaataaag caaatgtgat
    28801 ctaccgccct gtccatgaac tacccgattt gaaatggaaa gatgctggtg agcacatttc
    28861 tcaaacaatc tccaatctgt ctgtagacga atcgatttta gctgttttcc catatcaaaa
    28921 aattcaagca gagaaaacat ttgtattcat cagcaagaat ggattgatca agcaaacacg
    28981 aatgacagag ttcgaaccat ggcgcaccta taagagccgt ccgctcagct gtatgaagct
    29041 aaaggcggaa gaagatgaac tgattgctgt gtatctgaaa gaagatcagc caggcttaga
    29101 tgttttctta gtaacgcatc aaggaatggg tttgcgctat cctttaacag atgttcccgt
    29161 tgttggagca aaggcagctg gagtgaagtc tatgaatttg aaagatggag ataggattat
    29221 caacgggctg cttgtgttag cagaaggtga tacgccgatc gtaatcgtta ctcagcgagg
    29281 agctgttaaa cgcatgcttg ctcaagaaat cagccaaact tcacgtgcga agcggggagt
    29341 gacggtcatg cgtgagctga aaaaaaatcc gcaccgtatt atctatatgt cggaaggaag
    29401 atcaaaagaa ataaccttga tgaatcaaaa agggcagcag ttaacgattg acccaactga
    29461 tttccctata ggtgatcgaa catcgaatgg atcatttgcg atggatgaga aaaaaggtgg
    29521 agaagtcatc gaagttatcg atgctcctga gattttcatc gagtcgtaac tgttgtagta
    29581 ttttaatgaa gagacgccat ctgtattaat acaggtggcg tctctttttc attcattttt
    29641 aataaatcct tatacgtttg caaaaaaaga tgaaagtgtt cacaatgctt aaatgttggt
    29701 atgtcaacgt tgatttaaat atatgtaaat aaaacataaa aattgttaaa taaaaaacaa
    29761 gaaaaccctt gcaaagatag ggtgctgtgt tatactaatc acgtaaaaac tgttacataa
    29821 aacagaaatt taggaggtcc atacaatgga gcaatggaat ggatttaaag gtaccaaatg
    29881 gaaagaaggc gtagacacta gagactttat tcaaaataac tacactgaat atcgcggtga
    29941 tgacagcttt ttggaaggcc caacagaagc aacagaaaaa ttatgggatg aattgcaaaa
    30001 gttaaatgat atccaattcg agcgtaacgg tgtctatgat atggacacag aaatcgtatc
    30061 aactattact tcacacgatg caggctattt ggataaagat ttggaaaaaa tcgtcggtct
    30121 acagacagac aaacctttga aacaagcatt catgccgttc ggcggaatca atatggctaa
    30181 caatgcgcta aaaagcaatg gctatacacc aaacgatgaa ttgacacata ttttcactga
    30241 ctggcgcaaa acacataatc aaggtgtatt tgacgcttat actgctgaaa tgcgtgctgc
    30301 tcgtaaaaac aaaatcatta cagggcttcc agacgcttac ggtcgcggac gtatcatcgg
    30361 tgactatcgt cgtgtggcat tgtatggaat cgactatttg atggaacaaa agaaaaaaga
    30421 tcatgatatg actggttatc atacaatgag tgaagaagtg attcgtttac gtgaagaaat
    30481 cactgaacaa tatcgtgcat tagcgcaaat gaaagaaatg gctgcgaaat atggctatga
    30541 tatttctaaa ccagctgcaa atgcaaaaga agctgtccaa tggctatact tcggatattt
    30601 agcagcaatc aaatcacaaa atggtgctgc gatgtcaatc ggacgtatct cagcattcct
    30661 tgatatctat atccaacgtg atttggataa tggagtaatc acagaaaaag aagcacaaga
    30721 attgatcgac cacttagtaa tgaaattacg tatggtcaaa ttcgcgcgta cacctgaata
    30781 taaccaatta ttctcaggat tccctatctg ggctacttta tccattgcag gtatgggatt
    30841 agatggtcgt tcattagtta caaagaatga tttccgtctg cttcatacat taacaaatat
    30901 gggaccttca ccagaaccaa acttgactgt tttgtattct gaacaccttc cagaaggatt
    30961 cagaacatat gctagcagaa tcgctatcga aagttcttcg attcaattcg aaaatgataa
    31021 tcagcttcgt gaacaatggg gttctgatga ttgtgcgatt gcttgctgtg tatcaggaac
    31081 agttgtcgga aaagacatgc aattctttgg cgctcgtgct aacttggcta aaacactatt
    31141 atatgccatc aatggcggag tcgatgaagt gactggcgcc caagttggac cagaatatcg
    31201 tccaatcaca tcagaaatac tagactttga agaagtgaaa cataacttca tgaatatgat
    31261 ggattggtta gcagaactat atgtcaatac gttgaacatc attcattata tgcatgataa
    31321 atattcttat gaagcagctc agctggcttt gatggattct gacttgcaac gcacatttgc
    31381 tacaggtatt gctgggatct ctcatgcggc agacagcttg tcagcgatca aatatgcaaa
    31441 agtaaaagca atccgtaatg aaaaaggaat agcagttgat tttgaagtgg atggcgactt
    31501 ccctaaatac ggaaatgatg atgaccgtgc agatgaatta gtttcatgga tcttggaata
    31561 cttcatggga caaatcaaac gtcataaaac ttaccgcaac tcaaaaccaa caacttcatt
    31621 attgacgatt acttcaaatg ttgtttacgg taaaaataca ggaaatactc ctgatggccg
    31681 tgaagctgga aaaccacttg cgccaggagc aaatccaagt tacaatgcgg aacaaagtgg
    31741 gttattagct agcctgaact caacagctaa aatgaattac ggctgtgcat tggatggaat
    31801 ttcaaatacc caaacaatca atccttcagg tcttggaaaa gaacaagata caagaatcga
    31861 aaacttgcgt aatgtcttag atggatactt ctcaaaagga gcttatcatt tgaatgtcaa
    31921 tgtattcagt aatgatttat tgcgtgatgc aatggaaaat ccaatgaaat atccgaactt
    31981 gacgatccgg gtatctggtt atgcagttaa attccgtgac ttgacaagag aacaacaatt
    32041 agatgtcttg atgagaactt cacacgacag aatgtaaaaa gttcgtgaaa aaagtttttc
    32101 gacttagcgt cgaaaggacg ttgaatagcc atcgcatcaa aatcgaataa tcagtatgct
    32161 tatttgaaag aaacgattta caggggcagg acttgtatgt tccgtccctg taaattttac
    32221 acataacgaa gcggcaattg cgaaaaattt tgttcagaaa gcttcgcttc gacagctaac
    32281 ttgtacttaa aatgaaatca aagaaaagat gtgaacttaa atggaagaaa aaacaatcgg
    32341 ctatgttcat tctatcgaaa ctttcggttc agtagacgga cctggactgc gctttgtcgt
    32401 gtttatgcaa ggatgccgca tgcgttgcca attttgtcac aatccagata cttggaatat
    32461 tggcggaggg aaagaataca cagcagatga acttttagat aaagcagaac gcttcagacc
    32521 ttactgggga gataaaggtg gtattactgt tagtggtgga gaacctctat tgcagatcga
    32581 ttttttgatc gaactattta aaaaagcaaa agagcggaag atgcatacta cattggatac
    32641 atgcggaaaa ccttttacgt atgaagaacc tttcttttca cgttttcaag aattgatgaa
    32701 gtatacagat ttactgttat ttgacatcaa acacatcgat aatgaagagc ataaaaaact
    32761 aacgcattgg gacaatgaaa atattttaga aatggctcaa tatttgtcaa aaatcaacaa
    32821 accggtttgg attcgccatg tactagttcc tgagcgaagt gattatgatg aatatttgat
    32881 ccgactagac aactttattc aaaccttgtc aaatgtagac agagtagaga ttcttcctta
    32941 tcatacgatg ggaaaataca agtgggaaac acttggtttg aaatatccat tagaagggat
    33001 cgaaccacct acaaaagaac gtgtcgaaaa tgctaaacga ttgcttcata cagaagagta
    33061 tacaaaatat ttaacgcgat aggtcgcatc ggaaagaaga taagtattgc ttctttccgt
    33121 ttttttgcta ttattaaata ggagaaatca caaaggagcg aatgacatgt caaaaatttt
    33181 agtttttggt caccaaaatc cagatacaga tgcaatcggg gcagccattg cgttttcaca
    33241 cttgcaaaat gaattaggta aagaaacaga agcagttgct ctaggtacac ctaatgaaga
    33301 aacacaatac gcattagacc actttcagct atctgcacca cgagtagtag agtcagctaa
    33361 agcagaaaat gcagatgtca tgcttgtgga tcataatgaa ttccaacaaa gtatttcaga
    33421 tatcagcgaa gtaaatattt tagctgtagt agaccaccat cgtattgcaa actttgaaac
    33481 agctaatcca ttgtactatc gtgcagaacc agttggatgt acaagtacga ttattttaaa
    33541 actatataaa gaaaaccaag taacaattcc taaagaaatc gcaggaatca tgctctcagc
    33601 tatcatttca gatacattgt tgttcaaatc accaacatgt acgcctgaag atgaagcagc
    33661 tgcaaaagaa cttgcagcaa tcgctgaagt cgatctaaac acttatggac ttgaaatgtt
    33721 gaaagcagga acaaatctaa gtgataaatc agctgcggtt cttcttgatt tggatgcaaa
    33781 aagtttccca atgggcagtt ccaacgttcg tatcgctcaa gtcaatacag tggaccttaa
    33841 tgaagtgatg gatcgacaag cagagctaga agcagcaatg gaagatgaaa tgaaagcaaa
    33901 tggctacgat ctgttcgtat tagttgtcac aaacatctta gacagtgatt cagaattact
    33961 tgttgctggt aaaggaaaag aaaaagtaga agaagctttc caaacaactt tgaccaacaa
    34021 ccgtgctttg cttaaaggtg tcgtttcacg taaaaaacaa gtcgtacctc aattgacggc
    34081 tgtatttagc taatcaaatg gtagtatgaa gcattcaaaa tgaatggata agcgattgat
    34141 ataaaaagaa acatagaaca tactaaaggc cgaagaaatg gaggagaata acagtcaatt
    34201 atgactcttg ttcttcctcc tttttacgta aaagcctttt aagtaaaata aaacggatag
    34261 aaaagagaac atatatgaac tattattcaa aaaataagga aaacttttat caaaaactga
    34321 ccgatgatcc gcttttttct tcattgaccg attatctata tgaacatcga gaacaagaaa
    34381 cgattctaag agaattgaaa aaagaatttt cgcaaaataa attttcccat tttcttgatt
    34441 tattgatcga tgcaggatta atcaagaggg aagaacgaag atatcatttg aactttccaa
    34501 tatttgatcc taaagattac ttgcaacaag ccacttcggc tgctgaaacc atagctgaac
    34561 aattgaagag actctctgtt gatgaacaaa aattagcgat gggagaagtt atttgggcgt
    34621 attgtttcga agatgaacgg aaagaagcgt atttttatgg agtaagaaac agccgagaga
    34681 cagaattgct gaggacaact gcaggaaatg agaagtaccg ttttatcaca ctttcctcta
    34741 tagaacactt cccgcttact ttggctaatt acttttttgt acagaagaat cagcttcctg
    34801 taacgaaggc attcaaagaa ttagccgaac ttattggaga cgtaaatgaa tcgtactttt
    34861 ttgatcaaat agaagttatc gttgacagga tacgtaagaa taaatataaa aatcgccggc
    34921 ctagtatttt ccatcagtct ctacttgtga cgaatactat aaaggaagaa gaatcattca
    34981 cattagaatt acctattgtc gaaaaaaaca attttgagat tgaattacca acattggatc
    35041 cttcattgac aatggaggaa atggcctttc ttaaacgaca aatcttttct gagctgagca
    35101 agaaatttat accgcatgca ttttcttata tcaaagaata tggaacagtt ttagtgagta
    35161 aaacctaaga gagcagtaag caaaaattat actgactctt gcaggaggag cctttctctt
    35221 cctttatggt taaaagaatt atttgagtat tttgcctatc taccacctat tagattcact
    35281 acatggtctg gaaacaaagg aatgcaaaaa aatggtgaga aatgtggaat gacaaaggaa
    35341 gtagtgataa gaaaagttag atttttaaat aatcaatatt atgattcagt caagtatggc
    35401 attttatggg aagaactagc tgattagatt aggaagtcca tttgataatc cagaatcact
    35461 aagaaacgaa tggcgagtca ttaataattt atccaatctt gggttattct tttagattga
    35521 agtgtttaaa actaaaaaag catttatgaa gtcgtaatag tcaggcttca aaatgctttt
    35581 tattttgttt gttttttctt tgccacataa atagtaagcg ccccatagaa cagtacctgt
    35641 acgtcaaata acccgaccta tagaatagaa tcgggaatgt ttggcgtttt tagttacgag
    35701 aaaaatgttg ttgtacatcc ttgttccaag gcaacagttg atctaaagtt tctgctgaaa
    35761 gtacgggaag atttggtatc ttatcaaata gaaactctag atactttaca ggattcaatc
    35821 cgttaagttc tgctgtttta tatacactca gaatgattcc tgaagttcgt gctccttcaa
    35881 gacttgtgga atgcaaccaa tttttccgtc ctatcaccag ctctttgata tttcgctccg
    35941 cacgattatt tgtgagttct aaacgaccat cttcaaaaat aggttcaaaa ccacttcgta
    36001 gtttacacgc atattgaacg gctgcatcca acttggattt tgcaacagga tctatcgttg
    36061 ccatccactc ataaaaagca ataaataatg gacgcagttc ttgttgccga cagcttaatc
    36121 gcttctcagg agaaaagtct ttccatttct tttctaggac aaaccattta tccaattgtt
    36181 tgactgcttg agctgcggga atatccgtgt ttttctttcc ttttggaatt gcttcgaaaa
    36241 atttccgccg aacatgcgcg aaacaataga caagtcgtac actttccacg gcattatacc
    36301 cgctatagcc gtcacagtgt aaataccctg taaactcctt gagaaaagtt ttcggaacat
    36361 ctccagcacg actttcgtcg tggtgataat aaacgatggg atgctgactg tcttttcctg
    36421 tacaaaactg ccagtaatag gtatctgttt tggcactttc aatcacacga taacttgttt
    36481 catccccgtg tagaatctct tcttgattaa gatagactct taatcgttct cctaaagggt
    36541 ctaacgcgtt ttgcactgct ttaatatgcc aattggtgat attatctctc gaaatatcca
    36601 acccatgtgt ttgtttccaa tagttctctt gccgataggc aggaactttc tgaccaaatt
    36661 tcatccggat cgtttccgcc actaaactat tggaacctaa gctattagaa atcaaaggtt
    36721 taggaacgat cgctttttta aaagaagaaa gtccttcttt ttcgcagctt ttacaacaat
    36781 aggtatgttg ataatgggtg agacaatcca acatagcctg atggaaacga atttcttctc
    36841 gtacttttgt ccgtccgata tctttcatct gtgaaccgca atgttcacaa aattgttctt
    36901 ccaaaggaag gagatgttct ttctctgtta taggtaagtg tttgattttg tctgctttcg
    36961 ttcctacaac tcgttgatgt ccttttactg gaataatggt ggcagctgct tctggaacac
    37021 ttacagaaat ttcttcatca ctaaaaagat tcaattgccc attcgtttcc tcaaagagag
    37081 tctctttaga gcgtccaaat aatttgttgg ttaaataatt gaccttttca ttcaatagtt
    37141 ggatttcagt tgtaagattc tgaatggttt gttgttgtgc ttgattggtt ttatgggctt
    37201 cttgaagcaa ctgaagcaac gtatctgtag tttccaaaat ttttcaccac cttgaatcga
    37261 actgattttg ttacagaata ccacaagaaa cgcacaagaa ataggggttc aacacatttt
    37321 ttgtgttaac cccccagctt aaaaaactcc tttttttcct ggcaaaatag ctcgaggttg
    37381 atcgatactt aatccttcta acaaccatcg aacctgttgg ttcgttaagg ttcgaacttc
    37441 gtctttcgtc cgaggccatt ttaacttgcc gttttctatc cgcttataac agagaagaaa
    37501 ac
//
LOCUS       DAYYGY010000015        35803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000015 DAYYGY010000000
VERSION     DAYYGY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.015"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHA52_000857"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHA52_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8939605.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHA52_000858"
     CDS             complement(2020..2922)
                     /locus_tag="ACHA52_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8939606.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHA52_000859"
     CDS             complement(2919..3611)
                     /locus_tag="ACHA52_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8939607.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHA52_000860"
     CDS             complement(3654..4295)
                     /locus_tag="ACHA52_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8939608.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHA52_000861"
     CDS             complement(4333..4647)
                     /locus_tag="ACHA52_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939609.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKEAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHA52_000862"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHA52_000862"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8939610.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHA52_000863"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHA52_000863"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8939611.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHA52_000864"
     CDS             complement(5984..6916)
                     /locus_tag="ACHA52_000864"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8939612.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHA52_000865"
     CDS             complement(6913..7689)
                     /locus_tag="ACHA52_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8939613.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHA52_000866"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHA52_000866"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8939614.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHA52_000867"
     CDS             complement(10933..12009)
                     /locus_tag="ACHA52_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8939615.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHA52_000868"
     CDS             complement(12014..13294)
                     /locus_tag="ACHA52_000868"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8939616.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHA52_000869"
     CDS             complement(13313..14239)
                     /locus_tag="ACHA52_000869"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8939617.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHA52_000870"
     CDS             complement(14252..15535)
                     /locus_tag="ACHA52_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8939618.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHA52_000871"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHA52_000871"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8939619.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHA52_000872"
     CDS             complement(16323..17234)
                     /locus_tag="ACHA52_000872"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8939620.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHA52_000873"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHA52_000873"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8939621.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHA52_000874"
     CDS             complement(17700..18398)
                     /locus_tag="ACHA52_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8939622.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHA52_000875"
     CDS             complement(18385..18771)
                     /locus_tag="ACHA52_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939623.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHA52_000876"
     CDS             complement(18918..20588)
                     /locus_tag="ACHA52_000876"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8939624.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHA52_000877"
     CDS             20771..21004
                     /locus_tag="ACHA52_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8939625.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHA52_000878"
     CDS             complement(21103..21534)
                     /locus_tag="ACHA52_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8939626.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHA52_000879"
     CDS             21613..22026
                     /locus_tag="ACHA52_000879"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8939627.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHA52_000880"
     CDS             complement(22064..23212)
                     /locus_tag="ACHA52_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8939628.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHA52_000881"
     CDS             complement(23334..23930)
                     /locus_tag="ACHA52_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8939629.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHA52_000882"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHA52_000882"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8939630.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27590
                     /locus_tag="ACHA52_000883"
                     /pseudo
     CDS             27160..27590
                     /locus_tag="ACHA52_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(27635..28984)
                     /locus_tag="ACHA52_000884"
     CDS             complement(27635..28984)
                     /locus_tag="ACHA52_000884"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8939631.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29054..30217)
                     /locus_tag="ACHA52_000885"
     CDS             complement(29054..30217)
                     /locus_tag="ACHA52_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8939632.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30346..30669
                     /locus_tag="ACHA52_000886"
     CDS             30346..30669
                     /locus_tag="ACHA52_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939633.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30852..32201)
                     /locus_tag="ACHA52_000887"
     CDS             complement(30852..32201)
                     /locus_tag="ACHA52_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8939634.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32270..33223)
                     /locus_tag="ACHA52_000888"
     CDS             complement(32270..33223)
                     /locus_tag="ACHA52_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8939635.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33325..34515)
                     /locus_tag="ACHA52_000889"
     CDS             complement(33325..34515)
                     /locus_tag="ACHA52_000889"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8939636.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(35160..>35803)
                     /locus_tag="ACHA52_000890"
     CDS             complement(35160..>35803)
                     /locus_tag="ACHA52_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8939637.1"
                     /translation="LAHDQTGHGSDHTKRVVKLAEKILETEPQADRFVTLAAAYLHDT
                     IDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSKNLSEAAELSLEGKI
                     VQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKENYRKGTTVINHFYEK
                     LFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTPDNWIKNNKK"
BASE COUNT        10182 a         7825 c         6275 g        11521 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gcttccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aatttttgta ttagaacaag
    27241 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
    27301 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
    27361 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
    27421 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
    27481 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
    27541 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
    27601 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
    27661 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
    27721 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
    27781 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
    27841 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
    27901 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
    27961 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
    28021 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
    28081 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
    28141 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
    28201 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
    28261 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
    28321 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
    28381 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
    28441 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
    28501 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
    28561 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
    28621 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
    28681 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
    28741 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
    28801 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
    28861 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
    28921 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
    28981 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
    29041 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
    29101 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
    29161 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
    29221 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
    29281 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
    29341 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
    29401 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
    29461 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
    29521 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
    29581 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
    29641 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
    29701 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
    29761 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
    29821 ggaaataata tcttaaataa atgccaaatg gtatgagtaa taaaacatta aaagctggtt
    29881 ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac aagaccaata
    29941 ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc tttggccctg
    30001 tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa tacgcacaca
    30061 ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat gttccatact
    30121 ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat ggaaaaacaa
    30181 tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt gattctttgt
    30241 atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct attatttctc
    30301 atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct gaatatgtac
    30361 tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc ttatgtaaga
    30421 aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg tttcatgcta
    30481 gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat gactccacat
    30541 gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat tttcatttct
    30601 ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca gttacgcaaa
    30661 tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt gctatttcat
    30721 cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca tttcttccac
    30781 gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct aataaaattc
    30841 gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc aagtgtcaat
    30901 actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac gataaaagtc
    30961 cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta tggtcgaaac
    31021 aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt ataaaaaatc
    31081 caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat catcatgcca
    31141 atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt atcatgaagt
    31201 ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata agggagacgc
    31261 attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat accaactgtg
    31321 accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc cgcattgctg
    31381 tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac taataaataa
    31441 acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt cgccggcttt
    31501 ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa catcgttgct
    31561 agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg gatcggaaac
    31621 agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac aaacaaagga
    31681 ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga acctaaaaaa
    31741 ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat tgactgggaa
    31801 agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc tacatttgct
    31861 ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt tccgtaaatc
    31921 cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag ttcagctcct
    31981 gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat atgcaaactg
    32041 gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc accgatcact
    32101 gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa ttcattttct
    32161 atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc cctttcatac
    32221 catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt tataccgtct
    32281 ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct aagtcgtatt
    32341 tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt ggccaagatt
    32401 ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct ggtatgattt
    32461 tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata ttgaaggcat
    32521 ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca tcgatcgcat
    32581 ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg tactttcctt
    32641 ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct cctggcaagg
    32701 ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca tatttcacat
    32761 aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg gttgtaggac
    32821 gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac gcgccgattg
    32881 aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca ttgaccagcc
    32941 cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct gacaaaagag
    33001 cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc attttttcgt
    33061 aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca ggcatgcgta
    33121 tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg atcaactctg
    33181 aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc ctccctgatc
    33241 ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc tctaatttta
    33301 aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta ttttttgata
    33361 aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca tatttctgat
    33421 cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa tcccatgttc
    33481 gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga ctggagtaat
    33541 aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt taaaataatt
    33601 agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag taatcgaagc
    33661 caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg gccgacttct
    33721 agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa caacacctat
    33781 agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa aatgcttgac
    33841 ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt aggtgatcag
    33901 tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc catccatgga
    33961 gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta tcgcttcctt
    34021 tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt ttgctccgga
    34081 taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga tagcatcttc
    34141 ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt gaaaggcgat
    34201 tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat ggattttcag
    34261 cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg ttgcttcaat
    34321 acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg atgctagatt
    34381 gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta gtatgtcgat
    34441 cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat acattgttag
    34501 ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac ataccaaaca
    34561 tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg cttacactga
    34621 ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt caaatgactt
    34681 atatagtatg aacaattacc ttttagataa tgatctattt tactctacct gggcagataa
    34741 atacaacaca accattgata gaagcccttt cgttaaaatc aattgtattc tgaatattta
    34801 atttatttat tgcatagacc aataaaaata cataaaattt ccatcttcgt ttatcagcaa
    34861 tttttaattt tactctctga atatttctta aaagttttac ttgttgaatt cgaaaaaagg
    34921 actgtgacaa aagtcttgtc acagtcctat tcttccgaat aaacggtgtt ctcctgccaa
    34981 ttccgaatca caagttacaa tattcatcaa acacgagaag ctattttttg aaattgttcc
    35041 ttgtgactcg gagctgaacg gcaggctcac gacctcttgg taaacagtgt tccagaagta
    35101 acttttacgg aaaatgatcc gaaatatctt ggagctgacc acttctgtca caacctccat
    35161 cattttttgt tatttttgat ccaattgtct ggagtaaaat tttcatcgtc aatagaccat
    35221 tccagaaaaa attcttctaa gaattcacgc atgaaggttt ctcttcgttt ggcttcttca
    35281 tatccgtaat cagtattcat tttatcagac agcaagaaca gtttttcata aaaatgattg
    35341 atcactgtcg tgccttttcg gtaattttct ttgtccttga atgtttgcgg atacagttca
    35401 ggatcaaaaa tcgggtgccc atgtccaccg ccgaaataag cagtacgcaa aatgccgatt
    35461 gctcctaatg cttcgatacg atcagcgtct tgaacgatct taccttccag agatagttcg
    35521 gccgcttcag agagattttt agaaaaggac atattttcga taatggaaaa aatcatcgag
    35581 atctgttcct ctactattgg caaagagcga aggaatacac gaagctgttg ctttgcctcg
    35641 ttctcatttt taacgacttt atcatcaatc gtatcatgta ggtaagctgc tgcaagcgtc
    35701 acaaagcggt ctgcctgcgg ttccgtctcc aaaatttttt cagctaactt gacaactctt
    35761 ttggtatgat cgctcccatg tcctgtttga tcatgcgcta att
//
LOCUS       DAYYGY010000016        34432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000016 DAYYGY010000000
VERSION     DAYYGY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.016"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1147..1812)
                     /locus_tag="ACHA52_000891"
     CDS             complement(1147..1812)
                     /locus_tag="ACHA52_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939638.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1992..3974
                     /locus_tag="ACHA52_000892"
     CDS             1992..3974
                     /locus_tag="ACHA52_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8939639.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3978..4616
                     /locus_tag="ACHA52_000893"
     CDS             3978..4616
                     /locus_tag="ACHA52_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939640.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4644..5072
                     /locus_tag="ACHA52_000894"
     CDS             4644..5072
                     /locus_tag="ACHA52_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939641.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5529..6440
                     /locus_tag="ACHA52_000895"
     CDS             5529..6440
                     /locus_tag="ACHA52_000895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8939642.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6657..7094
                     /locus_tag="ACHA52_000896"
     CDS             6657..7094
                     /locus_tag="ACHA52_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939643.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7189..8073
                     /locus_tag="ACHA52_000897"
     CDS             7189..8073
                     /locus_tag="ACHA52_000897"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8939644.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8297..11464
                     /locus_tag="ACHA52_000898"
     CDS             8297..11464
                     /locus_tag="ACHA52_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8939645.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11532..12029
                     /locus_tag="ACHA52_000899"
     CDS             11532..12029
                     /locus_tag="ACHA52_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8939646.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            12048..12512
                     /locus_tag="ACHA52_000900"
                     /pseudo
     CDS             12048..12512
                     /locus_tag="ACHA52_000900"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            12509..13267
                     /locus_tag="ACHA52_000901"
     CDS             12509..13267
                     /locus_tag="ACHA52_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8939647.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13353..14093)
                     /locus_tag="ACHA52_000902"
     CDS             complement(13353..14093)
                     /locus_tag="ACHA52_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8939648.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14154..15407
                     /locus_tag="ACHA52_000903"
     CDS             14154..15407
                     /locus_tag="ACHA52_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8939649.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15729..15956
                     /locus_tag="ACHA52_000904"
     CDS             15729..15956
                     /locus_tag="ACHA52_000904"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8939650.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16079..16405
                     /locus_tag="ACHA52_000905"
     CDS             16079..16405
                     /locus_tag="ACHA52_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8939651.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16473..17186
                     /locus_tag="ACHA52_000906"
     CDS             16473..17186
                     /locus_tag="ACHA52_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8939652.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17496..18346
                     /gene="licT"
                     /locus_tag="ACHA52_000907"
                     /pseudo
     CDS             17496..18346
                     /gene="licT"
                     /locus_tag="ACHA52_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            18467..20332
                     /locus_tag="ACHA52_000908"
     CDS             18467..20332
                     /locus_tag="ACHA52_000908"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8939653.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20346..21788
                     /locus_tag="ACHA52_000909"
     CDS             20346..21788
                     /locus_tag="ACHA52_000909"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8939654.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            21896..22597
                     /locus_tag="ACHA52_000910"
     CDS             21896..22597
                     /locus_tag="ACHA52_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8939655.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22736..23248
                     /locus_tag="ACHA52_000911"
                     /pseudo
     CDS             22736..23248
                     /locus_tag="ACHA52_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            23259..23765
                     /locus_tag="ACHA52_000912"
     CDS             23259..23765
                     /locus_tag="ACHA52_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFM8939656.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            24176..24751
                     /locus_tag="ACHA52_000913"
     CDS             24176..24751
                     /locus_tag="ACHA52_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8939657.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24751..25191
                     /locus_tag="ACHA52_000914"
     CDS             24751..25191
                     /locus_tag="ACHA52_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8939658.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            25207..25908
                     /locus_tag="ACHA52_000915"
     CDS             25207..25908
                     /locus_tag="ACHA52_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8939659.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(26406..26831)
                     /locus_tag="ACHA52_000916"
     CDS             complement(26406..26831)
                     /locus_tag="ACHA52_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939660.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            27062..28387
                     /locus_tag="ACHA52_000917"
     CDS             27062..28387
                     /locus_tag="ACHA52_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8939661.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            28389..30689
                     /locus_tag="ACHA52_000918"
     CDS             28389..30689
                     /locus_tag="ACHA52_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8939662.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            30696..31667
                     /locus_tag="ACHA52_000919"
     CDS             30696..31667
                     /locus_tag="ACHA52_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8939663.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            31689..33086
                     /locus_tag="ACHA52_000920"
     CDS             31689..33086
                     /locus_tag="ACHA52_000920"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8939664.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            33242..33865
                     /locus_tag="ACHA52_000921"
     CDS             33242..33865
                     /locus_tag="ACHA52_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939665.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
BASE COUNT        11561 a         5631 c         7185 g        10055 t
ORIGIN      
        1 tggtaagtgt ttccttgttt gttttagttt aaactagtgt gtaatttaaa aatctatatg
       61 gaagggtctg tggggtaata cttaaataaa ggctaggaac ctgataacat caaggttcct
      121 agccttttta taataaaatg aagcgaaaat aatttctttt aaagagccga gattatcagc
      181 tatataagag tgaatttgat gcaaaaaaca gagccgaaac agtcttggtg ttcgtagctc
      241 tgcttatatg tttcgaattt aagacaaaga gttggcaaaa gatgttgata aggaagataa
      301 gataactgtg ttatataaac taggtaacta actgtaacaa gctaatttta agagattctt
      361 attagaagac gtttaggaat ataactaaga aaatgattat acaggtaatt gtcatttcaa
      421 taatgttttc taattttcgc gtctttgttt taagaaaaag gccagaatca gttggaaaaa
      481 agttcgtacg atagtgaaga ggggcaaaat ttcctactaa tattcccact aacaagagat
      541 aaaacggcca ggttggtgtg attcctctat aaaaaaatgc ccatgctacc gaaatcccat
      601 agattaacaa ctgaacaatt tttacaatga aaaaattgtt ctgccattac tcaaagtctc
      661 ttcctccttg tatcgtttat ttgtttcatt ttatcattat gataggcgct tgaacaatcc
      721 attttgtatt ttattgagta ctcttggata aactaccagc gattatcata tttatttatg
      781 ttactgtaag ccatcatata aaagtaaatg agcagatcat agaaatataa aaaagaacta
      841 aaattgatct aaattatgtg cagcttgact acgtaccaat ctatattact tactagattg
      901 gtcgttaatc tattcggatc gaatgagcaa aatatttttg tgaatgtcca tccttgatta
      961 tgattccaaa aagaatcatt gggaggctgt ggcgtaaata ttatactata agccagaaat
     1021 acctatcatt ctgaactttt ttatttagga actttcatct caaggctttt tcaattaatt
     1081 tgtacccctg ttttttggat aagatcatgt gcattgattt tttctatctc tacgttcatc
     1141 tcctacttaa tgcctatctg gctcctgagg gcttcttttt aaggcgtcgt catagtcttg
     1201 gctgaggatt tcttggtaat ctgagggaat ggcttcccaa gtgagcaggc gaacgttcat
     1261 gacgtgtttt cctttgtgtt tgattacagc atggtttttc tctttttcta cctctccagc
     1321 gtgaaaagac agccatgtac catggccaaa gatatcatta catacccaac gttcgatgga
     1381 attgtccacg tatcctacag ggagtgtggt atagccgatt tgtggtttca aaaaaggatt
     1441 gaatcgatcg atcgtttcat ttttgtattg ataattgata agaaaaagat agaccaaaca
     1501 aaagacgagc agaaagaaag atagtgtctt tttgagtgag aagggttgtt ttgatgaata
     1561 agatttcttg ttaacaagag gttcatctgg caatgttggt tctattgatt cggtagctga
     1621 ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga tgtaaaagtg acagcatttc
     1681 aaggtctggc attgtacgag aattttccca actagaaatc gttttatcgg aaacaccaat
     1741 tttttcgcct aattcttttt gcgtaagctt acaatttctg cggtaagttc gtattttttc
     1801 tccggtgttc atcttcttct cctttcgatt ctacagtata tagatttatt ctacatgacc
     1861 tatacacttg atagatacgt ttttttgatg aaaaacggta tgattttatc aaagaatcat
     1921 ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac agttttactc acaatcatag
     1981 tgaccggtat catgagtggt tttgccttgg ttagtataaa taaacaggta ctctatcgga
     2041 caaatgaatt tttccatcaa acagatactt atactccgat tcgattgcca caacatttta
     2101 ttgggcaaga agaatatgag cagctaatca tggtatttga aaatgtctca tcagaaactg
     2161 agctttctta tatgaaaaaa acagtaaatg aatccaatga agtagataca tcttggaact
     2221 tcaagataca gccaaagctt gaaatgacat tgtattatca aggggatgtc aaatcttctc
     2281 acgagaaggt accctattct aatacctcct ttcgcgtaac gtccattgaa gaatcgatca
     2341 aaataaaaga ttttgaaggt gaatttttct tgatgacgag agaaaaagaa gcctatgaac
     2401 ggttcatttc actattcact gatcgattca atcaagcatt tcacactgat tatgccacta
     2461 atatttttga agactttgaa aatagctatt cgtattcggt tatttcaaat acagacgtat
     2521 ataattatcg agtgtattta actattggat tgattctttt attccttttg ttatcatcct
     2581 ttttcttatt gatgaataaa gaaatcagtt tattgttgaa taatgacttt tcattacatc
     2641 aagtattgtg gtgtttattg gggaaaaaga aaatcattat tacattcata attgcaggaa
     2701 tcattggtgg ttttattttt atgtattata aggagcaatt tctttatttg ttccagatgt
     2761 acgttttctt gttcagcgga atatatggta tctctttgat ttctctttta ttagttgaaa
     2821 aaatcgcttc gttaagagaa gcgttcaact atatccaata cattcttttt acatttggat
     2881 tgttgttttc tcttcttttg accgttgcca atgtcgaatt agcaactatc gttttttctt
     2941 cttcacagtt agctgtttcc catgaaccac caaaagaaat cgctggtgag gactatcatg
     3001 tattttatcc agtaaccatc gggaaaaacc acgtggattt tatttattcc aatcgttttg
     3061 catcagcagc agatcaggaa ttatatgaaa ctttgaataa aaacggaagt atattagtca
     3121 atgtatcaga ttacttgaat gaagaaaatg aacagtttgg cagagggata aaaataaatc
     3181 tgaattatct taagaaattt cctttgattg atgtctctgg aaggttgatc caaataaccg
     3241 aaaaagaaac gcaccctgtt atattgatcc ctgaacgata tagacataca gatttaaaaa
     3301 atgacctggc gcaaataaca gaatattatc aagaaatcag tgaaaaagaa ccgaaatttc
     3361 tatttataaa aaacaatcaa cccatttata catttattcc ttctatcccc tggatcgaac
     3421 attatccggt tgttgaaata ctgacattga aaaacagtat gtatttggaa agaaatattc
     3481 tcagcggaga aatctatcca ccgttaaaga tcaagatagg ctctttgtcg aaagaaaggt
     3541 tatttgagct gattgaagaa agtcagttac gtgataactt gcagttatca tttccgtata
     3601 ctcagactga agagatagct gtcaaagtgt taagcagatc atttcattac ttgatacaga
     3661 tatttttact cactttcttt tctttcttct tgttgagcta tgtcatgtta tgcatatatt
     3721 ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag tggttattcg ctctttgaaa
     3781 cgtataaaga tttcttcatg atgaacctca tcaaatgggg aacgacaagt gtaatcttcc
     3841 tttttcttat agaaagagag cctaaatatc tttttaatat ttttttcttt agcttcattg
     3901 attttattct ttcagttgtt tttattttgt ctacagaaaa gaaaagtcag ttattgctga
     3961 tgaatggagg ttaacaattg gagccgttga tagtgataaa aggtgcgaaa aaaatcattc
     4021 gaaacaaaac gattcttcat ttggatcgtt tcactgcgga tgctggggat cttgtgactg
     4081 ttgttggaga aagtggagca ggtaaaagta cgttattgaa tatattagga ttgaatgatc
     4141 agttcagttc aggacaattc tatttgttcg atcaagagac gaaacaatta actgggagag
     4201 agaagcgctt gataaaaaga aataccatat cttttctttt tcaagatttt gggttgattg
     4261 aatatgaaac aatcgatttt aatttagata ttggattgaa gttttcgaat ttaaatgttt
     4321 cagataagcg aacagcaaag tatgaggcat tgaaaaaagt aaatataaac aagaagctaa
     4381 gtacgcctat ctacacatta tctggtggtg agaagcaaag aatcgcctgt gcgagagtct
     4441 tattgaagaa tagcaaaatc attttagccg atgaaccgac tgggtcattg gataaaagaa
     4501 ataaggataa agtagttgaa atcttatcgc aacaagcttc ccaaggtacg ttagttatcg
     4561 tagtcacgca tgatccatat ctgaccaaaa taggaaacaa gaagatcatg ctttaaaaat
     4621 caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat tatttgtgca ctagggatag
     4681 gttttgttgg aggaaacgct atgtcatctg aagtggaagc aaattcagtt cacggtgaaa
     4741 tctattacaa atctttaaag ccaggagaga ttcattttga tgctaaaggt aatattatta
     4801 agtcaaattt accagtagac cgggaaattt ctagaacagc aattaatcat agctctggaa
     4861 gatgggttta ttatagtgat acaacttgta cgagaaaaac aggtcattct aaccactata
     4921 gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg aaactctatt cgatcagcaa
     4981 atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg aaatagaact gacacattct
     5041 catgttggta caatccaacc ggttggtatt aagtatccaa aagtttatat aaaatagaaa
     5101 aaacatataa tgaaacagcg ttaaaagtta tttttggtag ttttaatata ttcattttta
     5161 gttgtataaa aatatatcat aataaaaaca acaaaaaaat gtctggaaaa tcgaacataa
     5221 aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa acaaaatcga taacatttgt
     5281 tctaagaatt agttacgtta aatacggaac gcaagataga cttgaaaaac ggtaacaaaa
     5341 gaagtagtat attaaggtgt gatgtgctac ttgacattta ttctgctgag cgaactttct
     5401 ggattgtgta gccttatatc tattcaggac gtatcgagca gattagtaaa ggtgttcttg
     5461 atgattttca tcttgaacac ctttgttttt agcaattcgc aaaaaacgaa aagtaggaga
     5521 attgataaat gaaaaaaaga tttttagcac ttgccatcgt actaggcact ggcttattgt
     5581 caggctgtac gaatgccggt gaaaaaacag ccgttagtta taaaggcggt acaatcagcg
     5641 aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc agattccgct gtgcaacagt
     5701 tgatcgttta tcaagtattt gaagataaat atggagatga cgtatcaacc aaagaaatcg
     5761 attctcaata tgatcaaaca aagaaacaat tgggtgattc atttgattca caattgaaat
     5821 ctgcaggtta cacagaacaa acattcaaag acagtatcaa acaaagtcta gcctttcaag
     5881 aaggtctaaa gaaacatatc aaattgaccg atgaagattt aaaaacggct tgggaatctt
     5941 tccatccaga agtggaagct caaattatcc aagtagcaag tgaagatgat gcaaaagatg
     6001 tgaagaaagc tgctgataag ggagacgatt tctctaaatt agccaaagac aagtcaactg
     6061 atactacgac aaaagaagat ggcggaaaag tgaaatttga ttccactact acaacggttc
     6121 cggcagaagt caaagaagca gcctttaagt tgaaagatgg tcaagtttct gatgtgatca
     6181 cttctaccaa tgcttcaact tatactacgg aatattatgt ggttaaaatg gtgaaaaacc
     6241 aaaacaaagg caacgacatg gataaataca aaaaagaatt gaaagaaatt gcaacagaca
     6301 caaaattaag tgacagtacc ttccagaata aagtaattgg cgaagtattg aaagacgcta
     6361 atgtaaaaat caaagacaaa gactttgaaa atgttttgag tacattcact tcagattcta
     6421 gtacagcaag tagtaaataa gcggtataaa taaaaaatat gaactattta aatcaataaa
     6481 actgttcatc taaatctttt tattagaact aaatcaacag tagtttaatt actacttgat
     6541 ttagttcttt ttattttaaa aatttttttt acttagaatc cagcgaccct agatattttt
     6601 ctgtataatc ttaaataaag aaaacaatat caaaaaatgc acgtagaagt agataaatgg
     6661 agtggttttg caaatttctt ttatttctag aagttattca cgtatacgga tttgtttttc
     6721 tcacaaataa aaaggcagga agtacaatgg atcatttgta tataaaatat attcatcaat
     6781 tttccgtttt ggtgttaggc acactaatta tagcggggat tgtcgcattt attattcaag
     6841 gccgtgacat attaacagta catcaagagt ttttcagtta tgcatttggt attcctctga
     6901 tcgctataag gtctgataac aaaaagtgga agaaacgcta ttacattctc ttgctatttt
     6961 tgtgttttta tctgccacct aagattttta ctttattagg tatacaatgc ctatcatttt
     7021 cttttatttc ctatatcatt gttttaattt tcctcagttt catagtatgg atgttttatc
     7081 ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca tcacagccta atttttttct
     7141 ttatgctatc gttatattaa agaaatcaat cagaagggaa agaaagtgat gaaaaaagtt
     7201 ttattagtgg taaaccccag ttcaggcggt gaacaagcaa aggaatttga gcaattagcg
     7261 atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct tgcatacaaa aaaagcaggg
     7321 gatgcaaaaa actttactcg cgaagcggct acggaagggt atcatagtgt gtttgtcatg
     7381 ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg ctgaacaaga acatcggcca
     7441 aatttcggat ttttcccatt gggtacggtg aatgacttgg ctcgagcatt agggataccg
     7501 ctagaacctg aggaagcgat caatcatttt tcgattgaat cagtcaagcc attggatatt
     7561 ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa ttggctctat ccctgaatca
     7621 atcaatgatg tagatgcgga gaagaagacg aaatttggaa aactagctta tttcatgtca
     7681 ggaatcaaac agttagcttc cacacaatcc tactcgtttc acgtagaagt tgatggaaag
     7741 aaggaagaaa ttgagagcag taccttgctg ataggactaa ctaactcagt cggcggtttt
     7801 gaaacattac ttccaaatgc aaaagtggat gatggtaagc tccatttggt ttatttaaaa
     7861 gattcttctt tgatcgatac gcttaaaaca ttgccagacc tattaaaagg agtagatgaa
     7921 tctacgaaaa atctagccta ccttacttgt gagcaattag ttgtgtcatt agctgatgag
     7981 gaagaattga cgacgaatgt tgatggagat gaaggggata aattacctgt gacaatcaaa
     8041 gtattgccct cacatttaca ggtttattgt taaaatggag gttgtgaccg aggagttggc
     8101 gggagaacac cgcttgttcg ggataaggga ctgtgacaag acttttgtca cagccttttt
     8161 tttgtacaaa aaagaaattt acacctaata gaaaaatttt tccataaaaa tacatatttt
     8221 aaatatgaaa gtacaattgg aaatagatta aaacgataaa ataaaattgt caaaacgtca
     8281 gagaggatgg tggagaatga atcaatgtgt aaaaaagctg attgcgtcga ttttgtttgt
     8341 cttccctctc ttgatggtga catccgtttt tgccgatgta gagaaaacaa acgaatcaac
     8401 gcaatcagag aatcaaagtt ttgacaagac tactttttca gaagaatcga ttacttctga
     8461 aactacagaa tctcaaagtg aggcttctac taataaaagg caagaagaaa ataagacaag
     8521 tgagacctca gaagtagctg aacaaacgtc tgatagcagt gaagcggcac tggcaagagc
     8581 tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa gaactggcgc agcttctcgc
     8641 agacgaatca gtaactaaga tccgactgat tcagcctttg actttagaca gggaattaga
     8701 gatcaaacga gatatcgtta ttgattttgg tggattcgct cacaatttcg gcacacacca
     8761 tatttatatc aatgagatac ctcagtcgat cgagttccag aattttaaag gaacagctgc
     8821 acatccaggt ggattgacac caaatatcga tggaaatgcc attatcattg cttatatgac
     8881 cgatttttgg ggaagcagat atcattttac gggagaaata aaatttaccg ggacacttga
     8941 tttgtatgat gggtccaaat taggactgat ttacgctcct cgtgcaactg tcacattaga
     9001 tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa ggactgaatg ccgctccggg
     9061 taaagcctat ttttgtagaa gctatcaatt gaatgtgatc aatggtagtc aactttatgg
     9121 accgtatcta gggaaattct acggttttct agattcagga gatgagactg gtagtggaaa
     9181 aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc tctcttgatt atgatcgggc
     9241 agatggcgtt agcgatgaag gagaggcagt cgatacgcta cctggtaatg tcatgtttga
     9301 agtcagtggt tcgggaagcg aattttcggt aaatacaaag atcaatatta ccaatgacaa
     9361 caatcgcgga atcatccaaa tgagaggttc aggatcgaaa gtcactgtat cgaatggtgg
     9421 gaaaatcacc atcaatacag caactacggg cggatttcgc ttgcaaggag attctagtca
     9481 aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg acgatcaacc
     9541 cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct ttgatcgtag aaggagccgg
     9601 atcagttatc gacatcgata agcagagtgg gcgttccagc gcagttcgtt ttgagaatgg
     9661 tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg gtaagaaata caggtgatgg
     9721 caaatcttac gtgaacagac tcaatcaagg aaatcaagcg atccaatttt atgacgcttc
     9781 tggttttcta gaaagtccag gttcagctga ttttacagtc actggagaaa aatcaaatat
     9841 cgatatacgc gcagattccg gagcaactgt agatacaact ggagatattg atttaaattt
     9901 tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa acaggggaag ataagaccgg
     9961 catcatcagt ggggatgtgt tgaatgtgga cttagaatct cctacctatt ttgatttcca
    10021 aaacaaacgg attggaagcg agaccaatgg cataggcggc tgggtattcc aaggaaatgg
    10081 agattcttct ttaaagatcg cccattcaga aatcgcttta tggcgaaaaa caggcgcaac
    10141 attcgataac tttgaaaaag acccggatta tttttctgat atgacagact tatcgatcaa
    10201 tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca ggtttaacaa atgaatttgc
    10261 tcagacaggt gccggaatga aaaattataa tcgaatcagt gccaataatc agctcccgat
    10321 gattgaatcc ttaagagtgc caacgaatgc agataaaaaa atctatgggc atgtcgttgt
    10381 tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag aagtgacttt
    10441 acgtattact tacgcagata cgacgaaatc atctgtagaa attactgctc tcacgcaagg
    10501 acaaacagat gaaaatgaag gggcaggata taacccatgg ggagaaggcg aacaaggcgg
    10561 cttgttcgaa gttcaagtac ccaatgatga gagattaaaa acggaggatc aagtgcaagt
    10621 tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca gaactggact ctgccaaaac
    10681 gacagtcgat gtgattcctc ctgaacctgc taatttaagt accacattga tcaatgcagc
    10741 ttctcgcacg atcacaggta caggtgaagc aggagccttg gtttcattga aaaaaaatca
    10801 agctgtttta tcagaaacaa ctattgatga tacaggggaa tttgttcttt ctatcccaga
    10861 aaatcaactt tttgcaggag atgtattaga aattgtatta aacgaccaag caggagaagc
    10921 tactgctaag ggtgtgcaga acaagccaag taccaacgat gaaattggga atcacaatcc
    10981 aagtgatacg ccagttgttt atcatgatgc cccgcctttt gcacctgcta cgaagatcgt
    11041 agtagaaggt ggtctctcat ttcttgcccc ttccaaactc gattttggtc agatcaaagc
    11101 aactggtttg acacaagaag catttggcca aatggatata caggaaaaat tatggattta
    11161 tgatcagcga gaaacgaaaa atccgtggat cttaacgata aaattatctc agcctttcac
    11221 aaatgatgca ggattttcta tgcaggatgt cctatatttc aagaaaggag aggagtatca
    11281 attagtgaat gaagaagaac tggaaattgt cagaggacaa aattcaacga atgtgcaagc
    11341 agattattca gaatatttaa ataacggtcg ggcgtttaaa cttgtcttaa ccaaagaaaa
    11401 tcagatagct ggtaattact cagcagagat aacatggaca ttagcagatg cgccgacaga
    11461 ataaagatca aggaggatga ttgatgaatc gaatatggaa taaaagtcat cgaaacaact
    11521 gctctttact tatgatcctt ctccttggat tcatcctttg gcagacacct gtccatgcct
    11581 ccgaattcaa ttttgcggta gattttgaat taccggctaa tcagattgat aagaacaaaa
    11641 catatataaa tgtaaggatg gagcctggga aaacacagga aatcccctac acggtcttta
    11701 accgaacaga taaatcagta acagttgaga cattgatcca ctccgctacg acgaattcaa
    11761 acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga tgaaacatta agttatgata
    11821 tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc tcctcaaagt gcggtcactg
    11881 agacactttc actgaaagcg ccagaaaaag agctggctgg tgtggtcgca ggagggatca
    11941 cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac agttggtgtc gtcaatcagt
    12001 tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa agagtcttcc ccagatttga
    12061 aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa tgtgattaag gcgaatcttc
    12121 aaaatccaaa tgcttcttat atgttcggct tgactacatc ggttgaaatc tacaaaaaag
    12181 aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat agatgtagca cccaatacaa
    12241 attttgattt acttgtccca ttagaaggaa aaaaattagc tccgggacat tatcaagcgg
    12301 ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca taccgatttt gaaataactg
    12361 atgatgcagc ggaccaattg aatgaaaagg atattgattt aaaaaatcag ccagaaacat
    12421 ttgactggcg atggctgata cttgttggag tcattcttat tggcttattt gctggattat
    12481 ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca attgcattaa gtaccgttct
    12541 agctggaagt ctattattat gtacacaaag tgtttttgct gagaataaga gctcagccac
    12601 tacagaagga acgatttctg tgaccaaacc agatgctgca aatcctgaag acgttgttcc
    12661 agttcctcct gttgatccaa atgatcctga tactcctttt acacctgacg aaccaaatca
    12721 gggaacatcc ggattactga cgattgacca agctccagat cttgattttg gaacaatcaa
    12781 gttaggtaca gcaaaaacag tatatgctgc attacaaagc ggaacggaca gtactggagc
    12841 acaaacacaa gtacagaatt tcgtacaagt aacggataaa agcggaaatt atgccggttg
    12901 gaaattatca gtcaaacgaa cagaatttat tggtacaaca tctgatacga ataaactaac
    12961 tggttcaaag gtgaaattca aaaacgccgt gattcgaaat agcgcaacta atagcagtgg
    13021 aatgccatct accgtttctg gaactggcgc tgaaggaatc gaaatcccag cagatcaggc
    13081 agtagatcta gtaactgcag gagcaaatga agggattggc acatggattt attcattagg
    13141 agagaatgtg gaacaaggga gacaatcagt ggaattattt gttcctgtat ccaattatgc
    13201 tgtagacaac tacgtttcaa cattcacatg gtcgttaaca gatgctccta cagaagagga
    13261 agcgtaaaat catatcaacg atttcaggga gtggagccga ttcaaggccc actccctgaa
    13321 atctgctatg cttacgtctc agctcttatt catcaactcg aagcagtttt ttggaagtca
    13381 tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa cgagtaagga gtcaccgttg
    13441 aaaaattcta acacataatg agcagaatca attttctgca attgcgataa gttgatacag
    13501 taagatttta aaagtaaaaa gtaagcatac ttttctaact ctgtcttcaa ttgttttaaa
    13561 gaagtattca ctaatttatc ttctgtagtt gttttgacca taacttggtt tttcaaacct
    13621 ttgatacttt caatatataa aatttcccga ggatacagga cgagacttcc acctttttga
    13681 tcaataaaga ttttatcgac atttacgatc tgctgttctt tccaattgat ctgttctagc
    13741 acttgacgaa tttgttcaga taaattcctg tcttttctga tatatccaat cggaaggatt
    13801 ttccgattga tgatctctac tgcaaaattc gtataagcgg tgaaaaaaac aatattacta
    13861 tgtgcatttt ttttacgtat ttgttcggcg acatcaatac ctgtgagtga ttgcttcaag
    13921 tcgatatcga taaagaaaac atcgtgatta tcaatagtgc ccaactggat caactgattg
    13981 atttgagaga tagaagtgag ctgcacgata ttctcgatat gatgattgac caattctctg
    14041 atgatccatt cgctgtgaaa ctggttatct tctatgataa aatacttttt catatgcagg
    14101 ctcctattaa aaaaatatgt gtacattttg aaacaaaaca atggaggaat aagatggtcg
    14161 caacagtgac actttatttg acctttactt tgattttaga tttaggtatc tatttattct
    14221 tttcgaaaca actgctagta aaaaacagct ttggttgtat agctttgatg gtggtgacac
    14281 ttttacttcc tatccctaaa gtgagctttc cttacctagt tggcttatta ttgatgtttc
    14341 ttttttattt tactacgaaa aacctgttta tcagctgttt gatcagtaat cttttttttg
    14401 ctatcactgg actcagcttt tttctggttt ttgatctgat ggattattta ggaaagagac
    14461 atcttggaac agcaagtttt tttgcactac ttatcgcatt tattttctat ctattagtaa
    14521 aaagtataga caaacagacg agatttctca acttgctttt tacttatcca actgaacact
    14581 attttggagg tatttgtatt ttattaagct ggggcgtgat ttacttggta ttgggaagtg
    14641 ttcacttccg ctcatctaat tatctgcttc tatctctttt gtcgatagtc atcagttttt
    14701 tttctttgtt tttttccatt atgttgatca gtttccaagt aaaagaaaga caacatcagg
    14761 aatcgcttca actgtatcaa ataaatgaag aatattacca tcatctagag gaattcaaac
    14821 atgattataa aagtctactg ttttcattaa aaattactct tcaggaaaat aaaaaagaaa
    14881 cgttggaatt tttagaaata ttagaagatc attctgcagc tatttttgaa aaaccgcaaa
    14941 tacaacagct aacaaatatt gtatccccag ctgtcagggg tgtcttttta aaatttctag
    15001 aaaaagcaga atcggagaaa atcccgatcc aagtagttat tcctgtcgaa gtgaaaacaa
    15061 tcccgattga tctggttgtt tttattcgct gtttatctat ttttatttct aacgcattcg
    15121 atcatcgtgt agccgatcag agtccgatta cgatcaatct gctggaagaa aaagagggat
    15181 cacgttttag tatagcgaac aaggcaaatc caacgaattt gacgctatcg gagatggtca
    15241 aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct atacagtgcg aaaaaaatgt
    15301 tggatgttta cgaaaatgca gaactaaaaa tcgaattttc aaaacagaat cactattttt
    15361 ttgtagaaat gtatctggca tctagaaaga gcaaatcagc ctactgactt actgtcaaag
    15421 gataagtttg aggaaagtct acacggagat agagacaaca acacagcata ttcaaaagaa
    15481 acagaagcgt gatgaccgtt gtcacccttc tgtttctttt gctatgtagt taaacaaagt
    15541 agttgacact tccgctactt agtattagta cgaaagtata cttcatgata ctaagtagat
    15601 aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa ctgctttgct tacaccagat
    15661 aaagggaaga gcaatatcgc ttattttagg cgtggagaag caagcaaatc cctacgatca
    15721 caaaaaaaat gctgacccag cagttaagag agttagaaca agatgaaagt atccatcgaa
    15781 aagcctataa ccaagtcccg cctaaagtag agtactcgct caccgaagaa ggaaaatcat
    15841 taagagagat tctagtagcg ttgtctgttt gggaagaaga acgtatcgaa aaacaaaaag
    15901 cctcaaggga agaagacacg ctactgcata cacatggtgg ctacctaaat tattgatttt
    15961 ctatgacatt tattttaaat aaaagaaaac aggagttttt ttaaaagcat catctatttt
    16021 ttatgtaaaa aaatggtatg attaatcaag taagaagtga ttggaagaag gcaagcaaat
    16081 gaatagtatg gaaaaagtca accagtttag agatgaacgt aattggcgac aatttcataa
    16141 cgaaaaagat ttggctatct ctatttcttt ggaagcatct gaattgttag aactttttca
    16201 atggaaaacg ccggaagaag taaaagaaac acaattagaa cgaatcaaag aagaactggc
    16261 agatgtattg atctattcat acatgatggc agacaacctt cacctggatc tagatgagat
    16321 cattgaagaa aaattgatca aaaataattt gaaatatcct gtagaaaaaa gcgcaggtgt
    16381 acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg ctgttggaaa gagcaaaaga
    16441 aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa aaaaggaaaa gcaaaaagat
    16501 ggctagtcaa tatcctcttg ttcctcttgt tattagtagg actagctttg attttcaatg
    16561 aacagatcaa ggattatttt gtcagagaga caggagataa atatgctata gcgaatgtaa
    16621 caaaagaaga tctgaaaaag aataacgata aagatgtcag ttttgacttt gatgcggtag
    16681 aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag aggaacagat ctaccagtga
    16741 ttgctagtat cgcagtccct tcagtttcaa tcaatttacc ggttttcaaa ggcttggata
    16801 atacgtcact actttacgga gcaggaacat tatctccaga tcaagaaatg ggaaaaggaa
    16861 attatgcctt agcaagccat cgtgcaacga accctgaatt gctgtttaca ccgctagaaa
    16921 atttagagat gggtgccaag atctatttga cagatttaga aaatgtgtat acatacaaaa
    16981 cattttttaa agaaaaagtt gctcctacag atacccaatt actgaatgaa gtcgagggaa
    17041 aagaaattgt cacactaatt acttgtggcg acatggacgc cgtcacgcga ctagttgtac
    17101 aaggagaact ggaaagtgtt acatcgataa aagatgcgac agatgatatg agatctgctt
    17161 tcaatctaga gacaaaaaca ttttaggata ctgggacata agtcgttaca gattaaaaac
    17221 agccaaagga tatgaatcga taaggaaaaa ccaatttgct gaaaaaaaga gaaacgcaaa
    17281 gaggtgcatc tctgatttgg ttgttttcta gatagacttg tgttccagtt tttttatttt
    17341 ttatatgaaa aggcttgcat aaataaaaat tcgatggtat gatgattttg aataagaaat
    17401 ggattgttac tgtacggcag gcaaaaccag aattcagcta agaaacagta ggcgaattct
    17461 aggttttttt atttttcttg agagggcgaa gcaaaatgaa aattgataaa attttgaata
    17521 acaatgtcgt catttcgaaa aatggattcg gggaagaagt cgtttgtatg ggaaaaggac
    17581 tagcttttca aaaaaagatt ggggacgaga tttcgccaga agcggtccaa aaagaatttg
    17641 tttaagagat tcttttgcta aaaaccaatt ccagcagctg atggctgata ttccggcaga
    17701 ggagatcgaa tgggtcaaac aagtagtcga actggctgaa gataaactaa aagttgaatt
    17761 ttcgcctaat atttatctga cattgaccga tcatcttcat tatgcgatca cacgagcaaa
    17821 agaaaatatc caactgccta atccattatt atttgaaaca aaaaaatttt atccgaaaga
    17881 atatcaagca gcgaaagagg ctcttttgtt cgtgaaacaa aagacgggtg tggaactttc
    17941 tgaggatgaa gccggtttta ttgcctttca ttttgtgaat agccaacaag gaaacgaaga
    18001 catgcaagtg acgatgaccg ctaccacaat ggtaagagat atactgagta tcatcagtaa
    18061 attcttcggc atcgtgttcg atgaagaatc tttgaattat cagcgtatca ttactcatct
    18121 gcagtttttt gcccagcgtt atctcaaagg ggaaggatct gacgagcaag atgaatttct
    18181 ctacgcatta gttcaaggaa agtatccaaa agcgtttcat tgtgtcgaac ggatcaatga
    18241 gtatcttcga caaacgcaaa aacaggaaat ggggatcgca gagcagattt atttaacgat
    18301 tcatattcaa agagtagtaa gtgagaaaaa aagcatccaa tcataaggat tgttactgta
    18361 tggcaggcaa aacctggatt gaaaaaatag gttcacgaat gtttgtgact gtttttttca
    18421 atctgggttt ttatttttta aataaaaaag aaagaaggaa aaaacaatgg ataatcaagc
    18481 agttggtcgt cgtgtatggg aagcggtagg aggacaaaag aatgtcaaaa gtttagtaca
    18541 ttgtgctacg cggttgcgat tcagattaaa agatgaatct ttagcagata cgcaaaagtt
    18601 aaaagaagac ccggacgtga tccaagtcgt acaaagtggc ggtcagtatc aagtagtgat
    18661 tggcagtaat gtagcagatg tttatcaatc gatcgtggat gaggaaggtc ttgcagtttc
    18721 ggaggatgga aaagaagagt ccaaaaatat tttgaatcgt ctgatcgata ttatctctag
    18781 tatatttaca ccttttctag gggcgatggc agccgccggg gtattgaaag gattcttatc
    18841 attggcaacg gtgatgggct ggctgacagc tgatagcggt acttatcaaa tattgtttgc
    18901 tgcagcagat ggtgtcttta ccttcttacc tgtcatgctt gcttttacag cagctaaaaa
    18961 gttcaaagcc aaccagtttt tagcagtagc aattgctatg gcacttgtgt atccagctat
    19021 cacagcagtt gctggtgcgg gagaagctat cagtttcttt gggatcccag tgattctttc
    19081 accatctggt tatacttctt ctgtcatacc aatcattcta gccgtatggg ttcaaagtaa
    19141 attagagcca ttcgtaaaaa aagtgattcc gcaatttctg caaatgattt tagttccgct
    19201 tgtcgtttta gtagtgatgg ttccattaac atttcttgct ttaggaccaa tcgggacggt
    19261 tgccggtaat gctttaggcg gtctcttcaa ctcgatttat ggatttagtc caatcgtagc
    19321 aggtctaatc atgggaagtt tatggcaagt attcgtgatg ttcggcatgc attggggatt
    19381 cgtaccgatc atgtttttga atatcgaaca atatgggttt gatgtattga tgccaatgct
    19441 tcttccagcc atcttggcac aaggtggggc tgctttagct gtagcattgc gaacaaaaga
    19501 tacgaaactt cgagcattag ggatttcttc tacagtgact tcgttatttg gaatcactga
    19561 accaactgtg tatggtgtga cattaccttt gaagaaacca ttcattgccg cttgtatttc
    19621 tggaggtatt ggtggagcta ttatcggatt ttccggagta aaagctttct ccagcagttt
    19681 ggtcagctta ttgacgattc cgactttcat taatacggtg gatggtgtag agtcaaatgt
    19741 gacagtagca gtaatagcta caggaattgc ttttgttctt gcattcgtag gtacattgat
    19801 tttaggattt gacgaacaaa cacaagacaa tcaattagaa aacaaacatg cgaatgcagg
    19861 agaaccgatt acttcagcac ggcatacctt aaaaagtcct ttaacgggta aagtactgcc
    19921 attgagtgaa gtaccagacc aagttttttc ttctggcgtg atgggaaaag gtatcgctat
    19981 tgatccagaa gtaggagaac tagttgcacc tgcagacgga gagatcacga ctatttttcc
    20041 aactggacat gctgtaggga tcacaacgac tgacggagcg gagattttga ttcatatcgg
    20101 gatggataca gtagaattaa atgggaatgg ctttgagatc ttagtcaaac aaggggatct
    20161 cgtcaaagca ggagatttat tgattcgttt tgacatcgaa gcaattagag cagctggcta
    20221 tagcgtaatc actccagttg tcattacgaa tactgatgca tttgccgata tacttgaatt
    20281 agaccaaaaa gagattattg caaacgaaga tgttctggca attgtaaaat aaggaggaaa
    20341 tcaggatgca atcacgtttt tcagaaaaat ttttatgggg tggagcaact gctgccaatc
    20401 aattagaagg ggcttatgat cgagacggaa aagggctatc cgttgcagat gctatgcctg
    20461 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
    20521 aaaaatatat ttacccgaat catcgaggaa ttgatcatta cgatcgtttc aaagaagatc
    20581 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
    20641 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
    20701 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
    20761 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
    20821 atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga
    20881 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
    20941 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
    21001 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
    21061 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
    21121 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
    21181 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
    21241 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
    21301 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
    21361 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
    21421 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
    21481 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
    21541 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
    21601 atttgatggg ctatacgcct tggggatgta tggatcttgt cagtgcatca acaggagaaa
    21661 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
    21721 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
    21781 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
    21841 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
    21901 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
    21961 aaaatatcca gtcggttcga tgttaccgac agaaagtgaa ttagaagaac tgtttcaagt
    22021 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
    22081 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
    22141 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
    22201 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
    22261 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
    22321 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
    22381 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
    22441 tttaacagaa gaagaacaag aaattctgaa aacagacgca cagatcggca tcaaacggat
    22501 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
    22561 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
    22621 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat attagcttct
    22681 catatttttc gtttggttag gcttattacc gcagataccc ggctgtttct ggattaccat
    22741 ttattctgga taagggggtg tggcacgatt ttcagaagga atctatgaac caatcactgt
    22801 attgatttta gcttcgatgg taaccccatt cggcttgacg atcgcttatt tcttagggaa
    22861 aatcattcgt aaaaatatat tcaatcgtca agaaatcgat acattgaaga ccgcttttcc
    22921 tatggggatc tgtcagatca cagagggatg tatccctatc gtattgaacg acttggcacg
    22981 caatgtcata gcaacgggag tcggtggggc agtcggcggt ggattgagca tgttttgggg
    23041 cgcagatagc cgtattccgg catcgggaat gtttgcagtc gctacgatga ccagaccatg
    23101 ggcattcatt ggtgcattgt tagctggttc ttttgctacg ggaataacta ttttattatt
    23161 gaaaaaacca gttgacccaa atgcagaagt catccaagaa gaaaaagagg aagaagagat
    23221 atcttgggat gacttgacaa tcagttagga ggaacagaat ggattacaag aaaagagtac
    23281 aacaaatgtt cctagattca ggagtagtat tgaaagaaac agaattagaa agaatcgaat
    23341 atacagattt tggattagga gcaatcgaga ctgaaggatt gaatttattt gtttatgaaa
    23401 ataatgagcg atactgtggg aaagaactgg tattattgcc ttatcaaact tgtccagagc
    23461 atcggcaccc acctcgagat gagaatgaag gaaaaaagga aacctttcgt tgtcgaaaag
    23521 gcttagtata tctttatgtc gaaggaaaag aaacaaaaaa actttcctgt attattccaa
    23581 aggggcaaga acgttactat acggcaaaaa aagaaatcat acttcacgct ggagatcagt
    23641 acacgatcga acaaaatatt aaacactggt tccaggcagg tgcaacaggc gcggttatca
    23701 ccgaattttc ttccagcagt gatgatccaa gtgatagttt tacgaatcca gctatttatc
    23761 gttaatacaa aggtttggga acaagctgat gaaccaaaat gatcaaattt cgattatttg
    23821 gttcgtcggc ttttttattt agagaaacat caggcgttgg tgaaacataa cacgagaata
    23881 aaatggattt ttcattaata ggagaaacaa gtgtgtgact atttaaataa tgcgtgttat
    23941 tgacatgcag gacgaaatcc caatttcttc tataagtaag tcgtttcaat tttatattta
    24001 ctagttattt atttttttgg ttactgtaaa atagctgaaa aattattttt ttgcaaatac
    24061 tatatatagt ggtgtttgat ctatatacca ctaaaaaact acaaaatcta gtcttttatt
    24121 ttaaaaaaac acgtgtagaa tgaagataga cttttgagtt agggagtgtt tttttatgaa
    24181 acttactgtt ttttgcggtt cccgttttgg caataaagaa agttacaaat ttattgcgca
    24241 aacgttagga aaatatatgg cacaagaaga aatcgaactg gtttacggtg gttcatactc
    24301 cgggattatg ggagtgatta gtaaaacagt actggaaaat aacggaaaag tcacaggtat
    24361 ctatccaaat ggcctttttg aaactgagct gcctgggaaa gatgttacta catttattcc
    24421 tacagagaca attgatgaaa gaaaggtatt attatttgaa aaaggagatg ctgtcctcgt
    24481 tttccctgga ggtctaggta cattggagga attttcgcaa cttctttctt ggatagcgat
    24541 cggcctgaca cctgataagc caatcggaat attgaatatt ggcggttatt acagcggctt
    24601 gcaacaatta ttagaaactt ttgcaaaaga ggattttatg gataaaaaat ggttggatca
    24661 agtgatcttt tccaacaatc cattggaact ggtcaatgaa ttacgagcag tatctattaa
    24721 aaatcagtta ttattgaagg aggcaaaata atgagtcaaa tatatttagc aggaccattt
    24781 ttttcagagg agcaaatcga tcgagtaagt cgtatcgaaa aagcgttgga ggaaaacaaa
    24841 acaatcacaa gtttctactc tccacgtcat caccaagaaa gcaattatga actattcagc
    24901 gctggatggg cgcaggaagt atacgaaaaa gatatggaag agttgacaaa tgcagaattt
    24961 gttttggcta tccttgactt tgagcatcaa acaatcgatc caggaacggc ttacgaatta
    25021 ggtgtggcaa ctatgttgaa aaaaccgatg atcatcgtac aagaagagac ggtacctacg
    25081 aacttgatga ttacacaaag tcttcacacg tatttaaaat cagaccaagc tgtacgtgaa
    25141 tatgattttg aaaaattgcc tgttgaaact tacgtaggcg agtacttata gaagaggaag
    25201 ggttgtatgt cagtgatttt gatttcagga acgattggtg caggtaagag cagtttgacg
    25261 gatatgcttg caaaagagat tgattctaaa ccattttatg aaaatgtcga agataacgaa
    25321 gtattgccgc ttttttattc aaatcctgag cagtacgcat ttttattgca aatcttcttt
    25381 ttgaataagc gatttttagc aatgaaaaat gctttagtga acgatgacaa tgtgctagac
    25441 cgctccatct acgaagatag tctgttgttc catctaaatg ctgatttagg tcgggtaacg
    25501 gatatcgaag tacagcagta cgatagctta ttagatacga tgctaaacga gttagatgat
    25561 gttgctccaa aaaaacgtcc tgatttgatg gtccatatca aagtttcttt ggacacgatg
    25621 ctagaacgta tcaaaaaacg cgggcgtgac tatgaacagc tagaaagcga tgaaacgtta
    25681 tacacctatt acgaaacact gaacacccgc tataatcaat ggtatgagga ttttgatgta
    25741 tgtcctaaaa tacaagtgga tggggataca ttcgattttg tagaaaatcc agaagatgca
    25801 aaaaaggtga ttcgtcagat ccaagaaaaa ttagctgaat tggaaggaaa gcctgctgga
    25861 aaatatttta cagataatca gcatgcgaaa ggtcaagaat taggctaaaa gtaaaaaacc
    25921 aaacgacggg aaagaaaaat tactacgtga tgtagaataa gtctttctat cgtttggtta
    25981 ttttatattc aaagatgtga aataaataaa acgttactct ttgatttgtg aactgaaatg
    26041 atccactgtt tgttttactg attccagttt ttgaagcttg atcaaaggaa gaagctgagt
    26101 ttgaagttca taaaaatcag ccatagtgag ttttgcttga ttttgcttat cttcttttgc
    26161 tttttacata atttttgaca ggataagatt caaacgattt aaaagaatct ttgactagtt
    26221 tgtggcaatt gtcgcatata ctcaaacttc cttgatcatt aaattctgta tggatatttt
    26281 tgttgttatt acatgtacaa taatcaggac tatcgatcat ccatttcgtc tcctttacag
    26341 tcatcttgct tctttactat agcagaaaag aaggggagaa acaaaagaag atccaacaga
    26401 tggcactata gactgtagat gttgccatcc ttatccttca aacggtaatt accgattttg
    26461 gtaaatgtga ctgttgcttc ttttttattt tcttttcctg aaataatata ttcgtcatct
    26521 ttggtagtaa tcttgatatc tgtcagcact gttttttctt cagatgattc agaagaatca
    26581 gctccttgtg tctgatacgt gcgtgtccat tccatttttt tatctgtaaa tgtcaactgt
    26641 cccgcggata aatccatatc tttacgtact tttagcgtat atacactgtt ggtgatatct
    26701 gctttttccg cttttaagac agaagaatcg gttgtttcgt ccgaggattg gtcagagccg
    26761 ccaaacaaat cacagccagt caatgtcaaa agtcccgcag acaaaataaa tagtacgatt
    26821 aatttcttca aaatacttcc cccatttgat aggataaaga aattatacca ttggaaagta
    26881 aaacttcaaa cggatggaag agaaaaaatt cacaaaatac cattaaatag aataattttt
    26941 caaaagtcat aaaaaatcca ccacctttag aaagcgtttt aaaaaaatgt gttgcaatct
    27001 ttaaaaaaag tgctatatta aaaatgaacc ggttcaattt tagaatctag gagatgaaat
    27061 aatgaaagtc gtagacagac tatctgaaaa aatgctacca atcgtatcta agatgagtag
    27121 ccagcgacac ttgctggcca ttcgagacag ttttatcgtt actatgccgt tagtaatggc
    27181 tgcttctgtc atggttttgc tgaatgctct gattttttca aatgcaacag tccaaagaat
    27241 acttgatcta agtccattgg ctgaacttgc ttcaatcgtg aataatggta cgatgagtat
    27301 tttagctatt atcgtgtgtt acaatatcgg ctggaatttg gcaacccact atatccaaac
    27361 aggcgtgatt gatgatccat catttagtcc tgttcatgca ggaattctaa gtacagccgt
    27421 aatgtttatt ttgatgtctt tatcaagtac agtcactttg atggacggaa atacagcaga
    27481 agctacaggg gttttcttac aaagtctgac ttcttcgtct ggcttagcaa cggcgatgat
    27541 tgctgccctt gtcagtacag aattatttat caaattagcg aaaatgaaga gttttaaaat
    27601 caggatgcca gaaggagttc caccggcagt agctgcgtca tttaattctt tagtaccaga
    27661 aatagttgtc attttatttt tttcagttgt cgtttttact ttaaaccata cgactggttt
    27721 gaatgttccg caattaattg aattagcgat tcagacacca ttgaaagctt ttgttttatc
    27781 cgtcccaggc atcttatttt tacagttttt ctctgatttc ttatgggtgt ttggtatgca
    27841 tggttcgagt attttggctc ctatacggca agcacctttg ttacagtcta ttcaggaaaa
    27901 catggatgct ttttcagtag gaaaagaaat tcctaatatt attacggagc cctttaccaa
    27961 tgcatttgga ttgatcggag gtggcgggtg tattttaccg ttagttattg ctatcttgtg
    28021 ggcttctaaa cgtcaagaac agcgatcaat cgctaaattt ggtctgacta cttgtttatt
    28081 taatattacg gaaccaatta tgtttggatt accagtagtc atgaatccaa tatatatgat
    28141 tccatgcgct attataccat ctattaactt ggttattgcc tacgcagcca caagtcttgg
    28201 aatcatcagt aaaacagttg ctgctgcacc ttggatcact ccaccggtta tacaatcttt
    28261 tattgctaca ggaggcgata tacgagcagc agtcttaaca gtaatactta tcattctaga
    28321 tgtattttta ttcctgccat ttgtattagc cgctaataag gcaaaattgg cagaaggagg
    28381 atattaaggt gtacgattac ccgtatcaaa acccaaagtt aaccatccgc caaagagtag
    28441 aagatttact ttcgaggatg aatattgctg aaaaagtagg acaggtaaac cagcatttgt
    28501 atggttggga atgttatgaa aaaaacggag acaagatcga attaacagaa aagttaaaag
    28561 aacatgttaa atggggaggc ggtctgggtg ccttatacgg tctgtttcgc tctgatccgt
    28621 ggtctaaaac agattataag aatgggattc ctgcaaaaga aagctggaag gtggctaacc
    28681 tagtccaaga atatgtacta cagcattcgc gatggaaaat tccagttttg cttgtagaag
    28741 aatgtccaca tggtcatcaa ggcttggatg ggatttctta tccaacgaat atcggccgtg
    28801 gaaatacttt caatgtagca ttattagaac aatcgagtcg attgatggca aaagaactgg
    28861 ctgctaaagg tgttcattta gcgctggtgt cgactttgga tctggcaaaa gatcctcgtt
    28921 ggggaaggac agaagagtgc tttggggaag atccgattct ctcagctcgt ttttctgaag
    28981 cggtcgttcg agggttccaa ggagagatta tttcagaaaa agtttctttt cttgatcaga
    29041 cagtagagga aatcaataag aagaaggatc agatcggtgt tgtgttaaag cactgtatcg
    29101 cacaaggaga agctttaggc ggtcataatt caggcacagt aacgattgga cgcagagagt
    29161 tctccgatat ttacggacca ttattgaaga gtacaagaaa tgcagtaggt gtcatggctg
    29221 cttataatga catcgacggg ataccttgtc atatcaatcg agcgcttttt gagcaaacat
    29281 taagaaaaga gatcggttat caaggaatcg ttatggcaga cggtgtggct ttggatcgtt
    29341 tatccgatgt ttttacagat aaaaagactg ctgcagcata tgcattagcg gcaggaatcg
    29401 atctcagttt gtgggatgag acctatacca agattgcaga agccattgat aatcaagtcg
    29461 tagatgaaaa gctactagat caagcagttc gccgagtttt atctgtcaaa tttttattag
    29521 gtttatttga acagccattt gcaaacgatc caaaggagta ttgggatcat ctcatggagg
    29581 aatcagagca cttaaattta gagacagcaa aagaaagtat cactttacta aaaaatgacg
    29641 gtatattgcc gttggatcaa caagtaaaaa acattgctgt tataggtcca aatgctcacg
    29701 atgtttacca cttgttggga gactactctg caccacaaac agaagaccgt ttgaaaaaaa
    29761 agatagtaca agagataaaa acagagtttc cccaatcaaa ggtttcttat gcacaaggat
    29821 gtgaagtaag aaatcaggag gatcaggaag aaaaattatt agaagcggtg acccttgctc
    29881 aaaactcaga tgtgatcatt gcagttctag gtggatcaag tgcgcggaac tttgatatgg
    29941 aatttcttcg taacggggca gtttcatcga aagggatcaa catggattct ggagaaaatg
    30001 tagatgttgc ttctctttct ttaggtggat accaagaaca gctgattgaa caattgtatc
    30061 aactaggcaa gccaatcatc acgatacttg tgcaaggaag accctacgat ttgatgaaaa
    30121 tcgagtcatt tacgaatgcc attgcaacag catggtttcc tggacaggag ggtggaaaag
    30181 ccattgcgca gatgatttct ggaagaaata acccaagcgg acgattgagt ttgtcttatc
    30241 ctagaaatag tcggcaacta cctgtttatt actatcagcg tgctgcttct aaacaagaga
    30301 attattatga tttaagcgga agtccatttt atccttttgg acatggtcta agctatacaa
    30361 catttgaata cagtcagatg gaagtcaaaa cggatattga caaagtaata atatcaatta
    30421 aagttaaaaa tacaggaagt gtttcaggta aaacaagtac tcttgtctat gtccgattaa
    30481 ttgacggcgt tgtcatccaa agaacgaaat tattgaaagc ttttcgtaaa gatgaattgc
    30541 ggtctcaaga ggaaagactg cttgtttttg aactcacttc tgaagacttt agttatatgg
    30601 atattgatga caagcggcat tatgcaaaga aagcggtcat catggtcgaa caacaagaaa
    30661 aagaaatcaa atgtaactgg caggagtgaa aatgtatggc atcgatccgt gacgtggcta
    30721 agatggctgg tgtttcagtg ggaacagttt cccgctattt aaacggtcag cagctgaagg
    30781 aaaagaatat ggaaaaaata tcagaagcga tttcagcgtt ggactataaa gaaaacatca
    30841 ttgctaaagg attgaagaat aatcgaagtt tctctattgg actgttgata aacgggatat
    30901 ctaaccgatt tggttcggag gttgtttcag ggatcgaaag agtagctgaa gaaaatgggt
    30961 atagcttatt attaagcggg tttgccgatc agccagaaaa aatcgaacaa aaaatcgaat
    31021 acctgatgaa gcatgcgatc gatggattga tcgtcttctt atcagaagaa gagtggtctg
    31081 gatttgaaat gctgacaaaa atgagtatcc cagttgtcgc attgaattgt ccgggtgggc
    31141 cagcaggtat tgatacaata gtagtgaatg atcgagaaag tgtagcaaaa gtgattgctc
    31201 atcatggcgg acaaggacat aaaacaatag gatttattgc tgccgctcaa acagattacg
    31261 tagcaagaga acgtttagct ggagccaaag aagcggtaca aaaggatccg tctatccatt
    31321 tagagatctt tacaggagac tattctagaa aaagcggcta ttacggggca aaagcattat
    31381 tagaaaaagg ggcaactgct atttttgtca gcaattacaa catgtcgatt ggtgccatcg
    31441 aatgtttcaa tcaagaaggt gtcagaatcg ggaaggatat tgcgttcagt cactatgatt
    31501 atttagataa aaacgatctg agtatccttc caaaaatcac gatcacgcca ccaacagata
    31561 aaatcgggga acgagcagca gaacagctgt tgaaacgaat caaaaaagaa gaggaagaaa
    31621 atgaaaaagt gattgtgatg gaaaatatca tacatggaat caactaaaaa agaaaaagag
    31681 gtgctttgat gaaaagatat caatttccaa aaaatttttg gtggggctct gcagcaagtg
    31741 gtccacaaac agaaggaaga atttcaggcg atggaaaagg agagaatatt tttgattatt
    31801 ggtacaaaaa cgaaccgcaa aagttctttg atcaagtagg acctgaaaaa acctcgcaag
    31861 tttatacgaa gtatcaagag gatgtacagt tgatgaaaca gactgggcat aattcatttc
    31921 gaacctctat ccaatggagc cgtttgatac ctgatgaatc tggaacaatc aacgaagaag
    31981 ccgttcgttt ctatcatcac tattttgatg agttgattga aaacggtatc gaaccctttg
    32041 ttaatctgta tcatttcgat atgccgatgt atctgcaaga aaaaggcgga tggttgaacc
    32101 gagaaacggt tgaagcctat gaaaaatacg caaaaagttg ttttcaattg tttggctcga
    32161 aagtaaaaaa atggtttacc cataatgaac caatcgttcc agtcgaggca ggctatttgt
    32221 atcgatggca ttatccggaa gaatcagata tgaaaaaggc aatccaagtt ggttatcatg
    32281 aagcgttggc ttcagcatta gccatcaaag cttaccatga aatggatcaa agcggtgtga
    32341 tcggaatcat cttaaatttg acaccgagtt atccaagaga cgaatcaaat cccgaagatg
    32401 ttaaagcagc acgaatagca gatgcgttct tcaatcgttc attccttgat ccagctgtaa
    32461 aaggcgagtt tcccagtgaa ttgattgaga gaataaaaga actcgatcta atgccggaga
    32521 ttcatccaga tgatttgacg atcattaaag aaaacacagt agatcttttg ggaattaatt
    32581 attaccaacc acgccgaata aaagcaaaag aaacaccaat cgatactaaa aacggtccta
    32641 tgcctgagga ttacttcgac aattatgaaa tgcctggtcg taaaatgaat ccttatcgcg
    32701 gatgggagat ctatgaaaaa ggaatctatg atattttgat caatgtacga gatcattatg
    32761 ggaatatcga ctgttttatt tctgaaaacg gcatgggtgt agaaaatgaa gcacgattta
    32821 tcaaggaaga tggtatgatc cacgatgatt atcggatcga atttgttcaa tctcatctga
    32881 aatatgtcca tcaagccatt caagaaggtg ccaattgttt aggttatcat atgtggacat
    32941 gcatggataa ctggtcgtgg accaatgcct ataagaatcg ttacggtttt atttcagtag
    33001 atttagcaca agatggaaaa agaacgatca agaaatcggg tcattggttc aaagatgtat
    33061 cagacagaaa tggatttgaa gcttaaagaa tagccaagag agaatgtatg aaaattcccg
    33121 ttcttaccgc ttcaggaaaa agaagttcaa ttatttacga tctttttcaa cttttttgtc
    33181 tttctatttc attttttgta tataatagaa tagtcaaaat taaataggag tggaggaagg
    33241 catgaggaaa aaaagaccgt ttcttctctt ttggttattc ggttttttct tactgcttat
    33301 tttatccgca gcattttcag gaaaaatgac agcagatctt gctcagaaag aaacagaaaa
    33361 aatcgtagca agcaaagaat cagtagataa tttcaaaaaa gaattctcaa cttattggtg
    33421 ggaggaatat aaattaactg ccaattacga aaaaggtgaa ttggacatct atttgccaaa
    33481 aggaaaagaa gaaaatcaat ttatcaatat cgatgacgta cttagtgcga tttctctcag
    33541 acaatatcaa ttcaaagaca aacatttggt cgttttgctt ttgactcatg atggcgacaa
    33601 ggtagcacgt ttgaaaaatg gaaaagtggt tacagcttat tctgaaaaag tggacaaaaa
    33661 agtggtggcc gatcgactag cggaatggaa aaagaccaac gaacaagtca agaaaacaac
    33721 tgcttcttct caaaaaaacg agtcgacaga actaagccct tcagattctg gcagtggaca
    33781 aacgataacg ggaaatgaag aaaatcaagc ctcaatcatt acgaatgagt catcagaagg
    33841 acaatatcaa acctatggac aatgaaaaac agaagttgtg acagaagtgg tcagcttcaa
    33901 gaaataagaa gaaatttacg aaaagtgctt ctcaagtttt tgtaaaattt cggcttattt
    33961 ccgaagaagc tacttctgga acaccgtgaa tcagaggttg tgaaatcagc ggtttgacct
    34021 gagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat
    34081 accgaaggtc agctgattga acaccgttta gtggagaggt tgtgaaaaag ctgtcctgca
    34141 tcaagaaata gggagctgcg acaagaattt tgtcacagcc tctttcctac gattagtcaa
    34201 gtaaaaggtg caagaaaaat agctcgtaaa cgaaagcgac aaaatcaagg cacactaaat
    34261 aaagccccgt tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt
    34321 tagttaatgg tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat
    34381 agcgtgcata catttcagaa gcttgttcat acaagcaact gtggcaacct ta
//
LOCUS       DAYYGY010000017        34419 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000017 DAYYGY010000000
VERSION     DAYYGY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.017"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHA52_000922"
     CDS             352..1041
                     /locus_tag="ACHA52_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8939666.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHA52_000923"
     CDS             1028..2236
                     /locus_tag="ACHA52_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8939667.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHA52_000924"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHA52_000924"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8939668.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHA52_000925"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHA52_000925"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8939669.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHA52_000926"
     CDS             complement(6339..7034)
                     /locus_tag="ACHA52_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8939670.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHA52_000927"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHA52_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8939671.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHA52_000928"
     CDS             complement(8233..8424)
                     /locus_tag="ACHA52_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8939672.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHA52_000929"
     CDS             complement(8445..9335)
                     /locus_tag="ACHA52_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8939673.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHA52_000930"
     CDS             complement(9322..10089)
                     /locus_tag="ACHA52_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8939674.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHA52_000931"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHA52_000931"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8939675.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHA52_000932"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHA52_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8939676.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHA52_000933"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHA52_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8939677.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHA52_000934"
     CDS             14660..15043
                     /locus_tag="ACHA52_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939678.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHA52_000935"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHA52_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8939679.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHA52_000936"
     CDS             complement(15987..16805)
                     /locus_tag="ACHA52_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8939680.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHA52_000937"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHA52_000937"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8939681.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHA52_000938"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHA52_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8939682.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHA52_000939"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHA52_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8939683.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHA52_000940"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHA52_000940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8939684.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHA52_000941"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHA52_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8939685.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACHA52_000942"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACHA52_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8939686.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHA52_000943"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHA52_000943"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8939687.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHA52_000944"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHA52_000944"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8939688.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHA52_000945"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHA52_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8939689.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACHA52_000946"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACHA52_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8939690.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHA52_000947"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHA52_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8939691.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACHA52_000948"
     CDS             28113..30086
                     /locus_tag="ACHA52_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8939692.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACHA52_000949"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACHA52_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8939693.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHA52_000950"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHA52_000950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8939694.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACHA52_000951"
     CDS             32965..34257
                     /locus_tag="ACHA52_000951"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8939695.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10502 a         6693 c         6817 g        10407 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgaca
//
LOCUS       DAYYGY010000018        34340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000018 DAYYGY010000000
VERSION     DAYYGY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.018"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(259..996)
                     /locus_tag="ACHA52_000952"
     CDS             complement(259..996)
                     /locus_tag="ACHA52_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8939696.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(998..2500)
                     /locus_tag="ACHA52_000953"
     CDS             complement(998..2500)
                     /locus_tag="ACHA52_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8939697.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHA52_000954"
     CDS             2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHA52_000954"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8939698.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHA52_000955"
     CDS             4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHA52_000955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8939699.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            7842..9107
                     /locus_tag="ACHA52_000956"
     CDS             7842..9107
                     /locus_tag="ACHA52_000956"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8939700.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9111..9842
                     /locus_tag="ACHA52_000957"
     CDS             9111..9842
                     /locus_tag="ACHA52_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8939701.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            9977..10861
                     /gene="rapZ"
                     /locus_tag="ACHA52_000958"
     CDS             9977..10861
                     /gene="rapZ"
                     /locus_tag="ACHA52_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8939702.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            10858..11856
                     /gene="yvcK"
                     /locus_tag="ACHA52_000959"
     CDS             10858..11856
                     /gene="yvcK"
                     /locus_tag="ACHA52_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8939703.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            11890..12825
                     /gene="whiA"
                     /locus_tag="ACHA52_000960"
     CDS             11890..12825
                     /gene="whiA"
                     /locus_tag="ACHA52_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8939704.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            13393..14049
                     /locus_tag="ACHA52_000961"
     CDS             13393..14049
                     /locus_tag="ACHA52_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939705.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            14228..14793
                     /locus_tag="ACHA52_000962"
                     /pseudo
     CDS             14228..14793
                     /locus_tag="ACHA52_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15492..16013)
                     /locus_tag="ACHA52_000963"
     CDS             complement(15492..16013)
                     /locus_tag="ACHA52_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8939706.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            16211..19891
                     /gene="nifJ"
                     /locus_tag="ACHA52_000964"
     CDS             16211..19891
                     /gene="nifJ"
                     /locus_tag="ACHA52_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8939707.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            20080..20967
                     /gene="cdaA"
                     /locus_tag="ACHA52_000965"
     CDS             20080..20967
                     /gene="cdaA"
                     /locus_tag="ACHA52_000965"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8939708.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            20964..22133
                     /locus_tag="ACHA52_000966"
     CDS             20964..22133
                     /locus_tag="ACHA52_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8939709.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            22157..23512
                     /gene="glmM"
                     /locus_tag="ACHA52_000967"
     CDS             22157..23512
                     /gene="glmM"
                     /locus_tag="ACHA52_000967"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8939710.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="ACHA52_000968"
     CDS             complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="ACHA52_000968"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8939711.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            25799..26089
                     /locus_tag="ACHA52_000969"
     CDS             25799..26089
                     /locus_tag="ACHA52_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939712.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26102..27127
                     /gene="sppA"
                     /locus_tag="ACHA52_000970"
     CDS             26102..27127
                     /gene="sppA"
                     /locus_tag="ACHA52_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8939713.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            27130..27789
                     /locus_tag="ACHA52_000971"
     CDS             27130..27789
                     /locus_tag="ACHA52_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8939714.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            27946..28878
                     /locus_tag="ACHA52_000972"
     CDS             27946..28878
                     /locus_tag="ACHA52_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939715.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            29032..30321
                     /gene="tig"
                     /locus_tag="ACHA52_000973"
     CDS             29032..30321
                     /gene="tig"
                     /locus_tag="ACHA52_000973"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8939716.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            30568..31818
                     /gene="clpX"
                     /locus_tag="ACHA52_000974"
     CDS             30568..31818
                     /gene="clpX"
                     /locus_tag="ACHA52_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8939717.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            31901..32488
                     /gene="yihA"
                     /locus_tag="ACHA52_000975"
     CDS             31901..32488
                     /gene="yihA"
                     /locus_tag="ACHA52_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8939718.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(32562..32717)
                     /locus_tag="ACHA52_000976"
     CDS             complement(32562..32717)
                     /locus_tag="ACHA52_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8939719.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            32853..34181
                     /locus_tag="ACHA52_000977"
     CDS             32853..34181
                     /locus_tag="ACHA52_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939720.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        11202 a         5939 c         7426 g         9773 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
       61 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
      121 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
      181 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
      241 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
      301 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
      361 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
      421 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
      481 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
      541 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
      601 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
      661 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
      721 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
      781 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
      841 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
      901 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
      961 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
     1021 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
     1081 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
     1141 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
     1201 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
     1261 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
     1321 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
     1381 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
     1441 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
     1501 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
     1561 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
     1621 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
     1681 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
     1741 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
     1801 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
     1861 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
     1921 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
     1981 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
     2041 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
     2101 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
     2161 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
     2221 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
     2281 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
     2341 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
     2401 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
     2461 ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc
     2521 aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa
     2581 tgaaaaaaat gcgagtttct aacattttat gttataaaac gtaacatgca taataagata
     2641 aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt
     2701 tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat
     2761 gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga
     2821 tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat
     2881 cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt
     2941 gaataaacca acactaatta ttgcccataa caaaacattg gctggtcagc tgtacgggga
     3001 attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta
     3061 tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa
     3121 tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt
     3181 gatcgtcatt gcatccgttt catgtatttt tggattaggt tcgccttttg agtaccaaaa
     3241 acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt
     3301 agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg
     3361 cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt
     3421 ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg
     3481 agaaacggaa cacgtggcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat
     3541 ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa
     3601 tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat
     3661 gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg
     3721 taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt
     3781 ggcggacgaa tcgcacgtaa ccatgcctca agtcagagga atgtataatg gtgaccgagc
     3841 gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc
     3901 acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc
     3961 aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg
     4021 gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg
     4081 cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa
     4141 aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca
     4201 tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt
     4261 tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct
     4321 tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca
     4381 aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt
     4441 aacagattct atgcgtctag cgatggatga aacatcaaga cgccgtacga ttcagcagaa
     4501 gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat
     4561 ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtatgaaga
     4621 aatgacgaaa gaagaaaaag atacgttatt gatgaaatta gaaaaagaaa tgaaaaatgc
     4681 ggcgaaagca ttggattttg aaacagccgc taatgtgcga gatatgattt tagaattaaa
     4741 agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcacg gtgcacgtgc
     4801 ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg
     4861 tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg
     4921 gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc
     4981 ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag
     5041 taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt
     5101 gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc
     5161 agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt
     5221 ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa
     5281 agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga
     5341 gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga
     5401 agggattcgt tctcgtctat ttgattcttt agaggcggct ctgcgattgg cagacggcta
     5461 tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc
     5521 ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt
     5581 tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt
     5641 gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag
     5701 ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga
     5761 tacaccattc gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga
     5821 tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt
     5881 cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg
     5941 tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt
     6001 gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt
     6061 gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt
     6121 aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg
     6181 actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg
     6241 aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga
     6301 accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat
     6361 gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc
     6421 agattatctg attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc
     6481 aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg
     6541 gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat
     6601 cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt
     6661 tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa
     6721 gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat
     6781 ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac
     6841 gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc
     6901 tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa
     6961 aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt
     7021 gcctgatgtc tatgttcctt gtgaagtatg tcatggcaaa cgatataact ctgaaacatt
     7081 agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaagatgc
     7141 tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt
     7201 tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca
     7261 acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact
     7321 agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca
     7381 aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa
     7441 aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct
     7501 tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt
     7561 gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa
     7621 tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc
     7681 tacactgatt ggtatagaca aaaacttcct ataagttcaa tccgttcaaa aaagaattga
     7741 gcaatcggag gagtttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag
     7801 ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa
     7861 atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt
     7921 cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta
     7981 cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtgt
     8041 ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc
     8101 ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt
     8161 cagaatattt tatctgtcca tatattgatt attctttatt tgaaagactg atcaataaag
     8221 taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca
     8281 gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg
     8341 ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag
     8401 cttttatttt agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg
     8461 gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg
     8521 tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgtctt ggtcatccac
     8581 tgttagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata
     8641 acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg
     8701 ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt
     8761 tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgtttcg
     8821 tcacagaaga tcgagtgttt aacaaaccct ttgttgaaac gacctatgga acgaatcaat
     8881 cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg
     8941 aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg
     9001 tgttttatga aaaagaccgg tcaatcaagc gctggttgtt gatgaaatat atgttccata
     9061 actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt
     9121 ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca
     9181 accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg
     9241 caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt
     9301 ttctgctgga tgatattgag aaacaaaatt tattgcagcc gaacttcatt gtgttgactt
     9361 gtaacaccgc acactacttt tttgaagaat tacaagcggc aacggatatt cctatccttc
     9421 atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga
     9481 ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg
     9541 gattcgaaac agtgattcct gatactgttc ttcaagaaaa aatcaattat ctgatctatc
     9601 atgagatcaa ggaatcggat tatttgaatc aagagctata ttacgaaatc ttagaagaag
     9661 cggtagagcg cctaaactgt gaaaaagtta tcttaggttg cacagaattg tcattgatgc
     9721 atgaatttgc tgaagataac cattatccag tgatcgatgc ccaatctatt ttagcagatc
     9781 ggacgattga acgagcgcta gctgaaagaa gcaaggcatt ggatacagca tcggaaaagt
     9841 aaaaaaattg gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa
     9901 agaaaaagaa aactggtaat gatacgcccg gaatgatata attttactat gcacaatgtg
     9961 agaggagttc gtaattatga cagataattt gcaattagtc atcataacag gtatgagcgg
    10021 tgcagggaaa acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa
    10081 tatgccgcct cgattgattc caaaattctg ggaattaatc aaagaatcag gcaaagtcac
    10141 aaaaatggct ttaggagtgg atttacgttc tcgttccttc tttgaagaaa tccaaaacat
    10201 gctgatcgaa atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc
    10261 agacgaagaa ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga
    10321 cggtttgatc actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca
    10381 agcttccgtg atcattgata cgacgacatt gacaccacga caattacgtg aaaaaatcaa
    10441 tgagtcattc aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt
    10501 taaatacggg ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc
    10561 tcattatatc ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt
    10621 gatgtccttt gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt
    10681 attgcctgga tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg
    10741 cggtcaacac cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta
    10801 taaagtcaat gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg
    10861 aaaatgaaaa cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt
    10921 ctgccagtta tcctgaaaag tttgcgaaat caaggagcag atatcacagc agtagtgact
    10981 gttgcagatg atgggggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct
    11041 ggtgacttga gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt
    11101 ttccaatacc gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg
    11161 atcattgccg cagtttccga gatgcgggga agcacttatg aggcgattca attattagca
    11221 aaaatgatgc atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat
    11281 gctgtcttta aagacggaac gactgcagta ggagaatcta aaatcgcgat cgaccgtaaa
    11341 accattgacc gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga
    11401 aaagtcgttt cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc
    11461 accagtatcc ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact
    11521 gctgaaaccg tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca
    11581 gatgccgatc atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta
    11641 ttggtcaata cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat
    11701 ctagtacaag tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc
    11761 accgactttt tggaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa
    11821 gaattgttcc gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag
    11881 gagaatagga tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat
    11941 cgagaacatg cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt
    12001 gtgaaccagc agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc
    12061 tattctcttt tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg
    12121 aaattaaaaa agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg
    12181 atggatcttg atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg
    12241 ggaaattcgc aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc
    12301 aataatccgg aaacgagccg ttatcatttg gaaatatttt ctatttatga acagcataac
    12361 caagacattt gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc
    12421 aatggctata tttcttattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt
    12481 ggtgcaacca actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat
    12541 tcggtcaatc gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct
    12601 tctaaacaaa tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct
    12661 gaaaaacttc aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa
    12721 ttaggggaaa tgattccctc aggtgcgata tcaaaatcgg gaattaatca ccgaattcgc
    12781 aaaataaatg aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact
    12841 gatacatgaa gcaggagatg cgaatgtatc agtttttttt aaaagttata cttgcttttc
    12901 ttccgtcaaa ctataattcc atctttactg gagtaacata atttggaaag gcttctattg
    12961 tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttgtac
    13021 ataaattttt gcttaatata gaagtcctga tatagataag atcaagtatt tatctataac
    13081 tggcctgatt aattaaaaaa ctggaaggaa ctgctcgatc atccttgaga actgctcttc
    13141 ttacttctgg tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat
    13201 tagctagtcg aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg
    13261 atttattcag tgataatcat aagttcataa atagtaattg agatgtatgg ttggaatacg
    13321 ttatacctga acttgaacta ccaagatttg gtttagaatt tgagaatcga tgtttataaa
    13381 gggagggcaa aaatgcgttt ttttacaact tttactatca ttatgattgc agtacttttt
    13441 atttttctag atatagcaaa gcgaaataca gcatttttat tatatcgggt tttacttcga
    13501 gcaggtctca taacatttat atcgatcgtt ggattttttc tattcacagt tattgtattt
    13561 atttggagaa ctccagcacc accattgcca gagattacat atggagaatt tccttttaga
    13621 cttgagtatg aattgaatga agaattacat gtgatagaag acacgttaat agttgaattt
    13681 gatggatttg gaatgaacga aggcataggg agatatcgta gatggacaag tcgtttagcc
    13741 agcggagaag atttagtgtt gctgttagaa gttagtgata ataagcaaat attttatttt
    13801 ccaggacctg ctaattatta catgggagat aggttgaatg gttataatca tacttttcct
    13861 agtgcttctt tcattgaaag ggaaagaggc attactagaa gggatatttt acatgacaaa
    13921 gaattattag aacaatttgg tccgcttgat caaaatacaa taaatgaaga agaattgtta
    13981 aatcaatata atataaggct agtgaattgg gaaatatctg aaccgattgt taacaatttt
    14041 ggtgattaga aaacaattac gataaataga aacaagccaa atcatcagca agtaaaagct
    14101 gatgatttgg cctttcttat acgagtagta ggaattcata gcgaatatta ttacgatcag
    14161 tagatatttt gatattaaag gagtattacg aactagtatg tgagaatgta gaggtgattt
    14221 gcgaataatg agaaatttat caaatagaaa caaaatatta atcattattg ttgttatagc
    14281 tgtgtttcat ctgggaacga atgcggtttt aagtagaata atattgggtc ctaagccacc
    14341 aagacctgcg attacccgag gggagtttga ttttagatta gaatatgaag tagatgggga
    14401 aaggattgtg atagaagata caattgttgc attatttgat ggattttctg cagatgcagg
    14461 tagcatggca tggtatcgca cgtggcgttt gcatttggcg agtgacaaga gggcacaaca
    14521 attattgtta catgagttag atgatggtcg aaaaatatat tatcattttg gaggagctgg
    14581 attttatatg gggaagttag tagcagtgta actgtaacaa ggaaagattt gccaggtctt
    14641 ggcattcgaa actccatatt ttatatcgag cgtgggagag atacatctat tgggaacagg
    14701 aaagggttaa ctcttgaaga gttatataat gactttggaa tccgtcttgt tagttgggaa
    14761 cacaatccac caatagaaaa tcgctttgaa taatcgaaat ctattaatcc atttcatcag
    14821 ccagtgaatg ttgtaatgga acaatttggt ttagtgttca aaaccattga aatactaggc
    14881 tgtgatcagc aaaaatgtat ctgaataaaa aagagagcaa ctattaaatg agaggagtta
    14941 ggagttgtta gctttatacg aatcaaagtg gcaaatacag atgcgtatgc atttgtgttt
    15001 gccatcttgc gtttggtaga cgagataggt ataagctaaa gaaacaactt gtttaaaata
    15061 aacgacctag ctcaacaaac gaccatctaa tcaactaaca gcatacataa aagcaactat
    15121 ttttgaagta gaattgaaat cgggataact taaaaagtga ttgggatact agtgtgaatc
    15181 accgtacata aatattactc gttaagggaa tttgagaaat tttgtatgaa aatttaagaa
    15241 ttagttagta tgcgcaagtc aaaaatatga ataaattatt tggtggagat aatttgctca
    15301 gcttgtcata agaaatgaga taatctggca gatgcaagct tttggacacc atttattcgg
    15361 aataaggagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct
    15421 tccgccgttt ataggtgttt agagcgtgag acaaaagtgt tctttacttt tgtcctacgc
    15481 tccttatttt tttattttgt ttggatcaat tcttttaatt cctctattga aatagattca
    15541 tcaaagattt ctttacccaa gatgacagtc ggaacaaatc gaatatgtgc ttgctcagct
    15601 tcgttgatga cagcttggct cagctgttcg tctttttgtt ctgtcagctt tagtttatct
    15661 actgcaaaat ccgctacttc ttggagagaa agatgtttcc actcgtcttg agtatcgaat
    15721 atttgtttga tctctttgat cgctttttga ccatcgctga ttgtgagata ccggtgcatc
    15781 acgtttcctc gctgcaagct ttctttttct ttatcgaaca atttgatcac tcgctgcaat
    15841 tgcccgcttt ggactgcttc ttctaatagc tcataagatt cttcaaacca ttgtcggcaa
    15901 tacggacaag ataggttgat gaattcgacc atttttttag gagcattcga tgaaccataa
    15961 aggattcctt tttgtgtatt tgttttcgtt gcatcaatca ctgatatatc catgttaaaa
    16021 cacctctttc gcttatagtt tatacatgtc tgacaaaaaa aggcaacgat ttagactgtt
    16081 tgtctaaaaa ttagatgacc aattcaaata gacatatgtg aaatattttt ataaataaaa
    16141 gaaaaaatgg aatgttttac gttataatgg aacagataat aagagagaat atgaacaagg
    16201 atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat acggcagccg catatatttc
    16261 ttatgcattt accgagattg ctgcaatata tccaataaca cccagttcga ccatggctga
    16321 attagtagat caatggtcag cagcaggaaa agaaaatgta ttcggtcaac cagtaaaagt
    16381 agtagaaatg caatccgaag caggagcagc tggtgtggtc catggatcat tgaaaactgg
    16441 tgcactgaca actacatata ccgcttctca agggctgctt ttgatgatac cgaatatgta
    16501 caagattgct ggggaactac tgccaagtgt tttccatgtt gctagtcggg cagtgacgac
    16561 aaatgcgttg aatattttcg gagatcacgg agacgtgatg gctgcaagac aaacaggatt
    16621 tgctatgtta gctgaaagca gtgtacaaga agtgatggat ttagcacctg ttgcccatct
    16681 tgcttctatt gaaggagaag tacctttttt gaatttcttt gatggattcc gtacgagtca
    16741 cgagattcaa aaaatcgaag ttttggaata tgaagaatta gctcctctga tcaatcagga
    16801 gaaattagct tcatttcgta aacgagggat gaatcccaat catccgactg ttagtggtac
    16861 gaaccagaat ccagacatcc attttcaaca acgggaaacg atcaacccct attatgataa
    16921 attacctgaa atcgttcaaa aatatatgaa agagatcaac cgattacgag gaacttctta
    16981 tgacttggtg acttattacg gagcagacga tgcggaagaa gtcattgttt ctatgggatc
    17041 agttgcacag acgattgaac aaacgattga ttacttgaat gcaaacggac ggaaaacagg
    17101 ttttttgaat atccatcttt atcgtccttt tccagtggaa acattcgtgc aaaagttgcc
    17161 tcatacagta aaaacaattg ctgttcttga tcgaagtaaa gaaccagggg caggtggaga
    17221 acctctctta ttagacgttc aaagtgcgtt atatgattct gaacttcgcc cggctgttat
    17281 tggtggtcgc tacggattgg ggtctaaaga tgtgactccc gaccaaatct cagcagtttt
    17341 tgatgaattg aaaaaagacc caagcatacg caaaaaacgg tttaccatag ggatcgtgga
    17401 tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg gatttgacag aaccgcaaac
    17461 attccaggcc aagttttggg gatttggatc tgacggaaca gttggagcaa ataaatcagc
    17521 tatcaagatt attggagatc acaccgataa atatgcgcaa ggctattttt attatgattc
    17581 gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt ggagatacgc cgatacgttc
    17641 agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac acaccagctt acctgcatag
    17701 ctatgacttg gtcaaaggat tgaagccagg tggcattttt ttattgaata ctctttggag
    17761 cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa cgttacttgg ctgaaaataa
    17821 cattagattc tatacgatca atgccatgag attggcccaa gaagtcggtt tgggacgccg
    17881 gatcaatacg gcaatggaaa cggctttttt taaattagct gacatcattc cttttgatga
    17941 ggtgcttccg ctgctaaaag aagaagccct gaaaagctat ggacataaat caatggctat
    18001 cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa ctgcttcatc aagtcgcgat
    18061 cccagaccat tggagaacgt tggagatgcc tgaaaaaatc aataaaagga acactggtta
    18121 tatacaagaa atacttgaac cagtgaatgc acaggaagga aatcaactct ctgttggtac
    18181 acttgtagca aatggaatga caactggcga aatgccttta ggaacggctt ctatggaaaa
    18241 gcggggcgtc gctttagaag tacctgagtg gattagtgat cgctgtacga tgtgtaatga
    18301 atgtgccttt gtttgtcctc atgctgccat ccgtccattt ttagcagatg aagaggaaat
    18361 ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga ggcgcagacg gcgtaaaata
    18421 ccgtatccaa gtttcagtgg aagactgtac aggttgtggt ctgtgtgtag aggcttgtcc
    18481 cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa cagaaagaac aagctgtgaa
    18541 ttgggctttt gcaatgacat taagacaaaa agcaaatcca gtcaaaggaa agaatactgt
    18601 cttgtctaca caattcaatc aaccgcttct tgaattctcg ggcgcttgct caggctgtgg
    18661 cgaaacacct tatgtaaaat tattgacaca aatgttcggt gatcgaatga tgatcgcgaa
    18721 tgcgacaggc tgttcttcta tttggggcgg tgcttctcct gcttctcctt atacgaccaa
    18781 tgaatgtggt caagggcctg catggtcaaa ttctttattg gaagataatg ctgaatttgg
    18841 ttatggtatg cttctagcgg cacagactcg tcgcgaggct ttggcaagtt caatcaaaga
    18901 agtattgcca aaagtatcag aagaactacg agaattgatg gaagactggt tggtacacat
    18961 gtttgagagc gaaggaacgc agcaaagagc agcaaaactg caagctgcga tcgaaagcga
    19021 acgatcaatt gtccctgaac tggaggatat atacaagaga aaggatctat tcgtaaaaaa
    19081 cagccaatgg atgattggtg gagacggatg ggcttacgac attggttttg gcgggatcga
    19141 tcatgtactt gccagcggag cagacgtgaa tattcttgtt ttggataacg aagtctattc
    19201 caatactggc ggtcaaacct caaaagcaac accagcttcg gcaatcgcta aattttcggc
    19261 tagtggaaag tatgcttcaa agaaagatct aggaatgatg gcgatgacat atggaaatgt
    19321 ttatgtggcg caaattgctt caggtgccaa tcaaatgcag acgataaagg cttttgaaga
    19381 agcagaaaag tatcctggac cttctttaat cattgcctat acgccttgta tcacacatgg
    19441 tttgcttggt ggaatgaaag aatcgcttaa agaagcaaaa gaagctgttt cttctggcta
    19501 ttggtcatta tatcgatata accctgcaat ggtcgaaaaa aacaagaacc caatgacttt
    19561 ggactacaaa aaacctgatt tctctgctat gcctgatttc atgaggaaac aagttcgctt
    19621 ttcctcgcta gagaacgcaa atccaaatct tgcaaaagct ttatttgaaa agactatcca
    19681 agacgctaaa acacgttttt acaactatgc tgcactgacg ggagaattag aaaaaatcaa
    19741 gaaacgactt gaaccagagg aatctatcga tgtacccgtt aagactagaa aagaaaaaac
    19801 gacggacctt gaagcagaag aaagaagagc tgccagagca gaacgacgta aaaaaatgga
    19861 acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat aattctctga
    19921 agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt tcctgaaaat
    19981 tatgcgtttt tctcaagaaa aatacgagca gatcagcctc taaaattaaa acatatgata
    20041 tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca aatcggggaa
    20101 ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc tctgaatttt
    20161 ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact gatccaactg
    20221 ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat tgttatccga
    20281 atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca agtcattacg
    20341 tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg actggaacat
    20401 cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga tgagaagatg
    20461 atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg tgcgctaatt
    20521 actatcgaac ggcatactgg attagatgag tatattgaga caggaattgc attagatgca
    20581 gatatcactg gagagctgct gatcaatatc tttattccta atacaccgct gcatgacggt
    20641 gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc tctatcagaa
    20701 agtatgttga ttccaaaaga attcgggact cgacaccggg cagctgtcgg tataagcgaa
    20761 gtcagcgacg cgattaccat cgttgtctct gaagaaactg gggatgtcag cattacatta
    20821 gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatttt gcgtagagaa
    20881 ctgattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt
    20941 atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc attctttaca
    21001 gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc
    21061 caaatacgct ttctggaaat gaagcgtata atcaaacagt tactggggtg ccaatcaaga
    21121 tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg actgtcaaac
    21181 tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca cggatgttcc
    21241 gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaggttccg ctaaaaattc
    21301 aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca gtaacggttg
    21361 agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat
    21421 ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc actacaggtg
    21481 acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg atggtcacag
    21541 atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa
    21601 gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag ccaagcaagt
    21661 cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa agctatgatt
    21721 tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt gcatcgatcg
    21781 gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa
    21841 ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt gtggagatta
    21901 cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg acacaagaaa
    21961 atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca tcgacaacaa
    22021 ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca gaagaaacat
    22081 cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat taagttaaag
    22141 gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa
    22201 caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag
    22261 ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata caagaatctc
    22321 aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta tcgaagtgtt
    22381 ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc aaaaagcaag
    22441 cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa tcaaattttt
    22501 cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag cattattaga
    22561 tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg tagaagaatt
    22621 tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg gtgacctttc
    22681 cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt
    22741 gtttgccgat cttgaaacag atttttatac catgggcaca aatccagacg ggttaaatat
    22801 caatgatggc gtaggctcta cccatccaga acgtttagct gagatggtcg ttgaaaaagg
    22861 agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg tggatgaact
    22921 tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctagatact tggcagaaaa
    22981 aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag gattccacaa
    23041 agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc gttacgttgt
    23101 agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc acatgatttt
    23161 cttagatttt aacacaactg gagatgggat gctttcagga attcagttac tgaatatcat
    23221 gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct atccgcaaaa
    23281 attagtgaat atccgagtaa gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa
    23341 agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag ttcgtccttc
    23401 cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga aagttaatta
    23461 ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt aagagatatt
    23521 ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt ccttgttctt
    23581 ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt cgaatccccg
    23641 taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta gagtagttag
    23701 ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc cctctgagac
    23761 aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc gagtatgcgc
    23821 accagtcact tcttcactga tgattccaat cactggtgtt ccttcttcga tcaaagcaat
    23881 cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac tgatttcttt
    23941 caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg ccacgtccaa tgtaaaaggc
    24001 atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt tttgggcgat
    24061 gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga atacaagaga
    24121 agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta cagctatttg
    24181 tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca atagcagtgt
    24241 aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa gtgaagggta
    24301 gccgagacgg ttgattttta ccagaacttg gcggctatct gctgtttcgc cactttgact
    24361 caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa attcactggc
    24421 taaatgaacc tcaacaggga tctgtgtcaa tttttccaga gtctgtttac ctgctaaccc
    24481 cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag acaatgtatc
    24541 gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt gagcgatacg
    24601 acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat aggtaccttt
    24661 ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa cgttctacga tgtcacctgc
    24721 aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat cttcaatctc
    24781 aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca cattgaatcc
    24841 atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa catcaggtgc
    24901 tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag cttttaaaaa
    24961 cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga taatctcagt
    25021 atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt cttcaaagtt
    25081 ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt tgggaagtat gtggatgggc
    25141 gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta tgaccaatac ctatatttcc
    25201 tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg ctttgaccag
    25261 ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac ggtattctaa
    25321 tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca ttccaacaat
    25381 tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct tccagctgca
    25441 ggaaaatcct tttgttttat ctttgcagat attttttgtg gattttctgt agagtgcagt
    25501 ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg tttacacaga
    25561 cctggcgcta tcctaaaagg tataactatc ctcctctttt gttttttatc tggaaacatt
    25621 taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa aataaattgg
    25681 tatatatcaa atgctttgtc aaatgctctt tttatgtcaa aaaataatgg gtttttatct
    25741 atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag gagtaaaaat
    25801 ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt ccaaattcga
    25861 agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct gggatagcat
    25921 ccaagaaatc gcacctgatg ccttggaagt tgctgaagct cgtgagttta cggtagaaga
    25981 tgaattgaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag agcggttcga
    26041 cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga aaggagaaag
    26101 aatgaataag agacgttggg cagcagttct gattgctgca ggattatttg tggtttcgtt
    26161 agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat tgaacagttg
    26221 gttatatggg gatgaagaac tagcttcaaa tgtgttggaa gagggagaca gtacgcaaaa
    26281 aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag gtttatttgc
    26341 ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg aagaagatcc
    26401 aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct acgaaagtgc
    26461 ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa tgtatgtcag
    26521 catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag ataaaatctt
    26581 tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat tgaattattc
    26641 aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag cgttgaaaga
    26701 tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc aagcatatat
    26761 cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata aatctgaaga
    26821 agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa aagaagtagg
    26881 tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatgcgaa aggaacaaca
    26941 gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg ctagtacatg
    27001 gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta gtcagatctt
    27061 atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt attacggagg
    27121 tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga taagcaacag
    27181 cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc ttttgaaaga
    27241 gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa gtatacggac
    27301 acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc aggttttttt
    27361 atccgaatgt ttgctttctt ggtcgatctt ttgtgtatcg gagctattac gcgtttaaca
    27421 cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct gagtatctac
    27481 ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa attcaatcat
    27541 ggtcaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga tgaagaagaa
    27601 cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct aaaatttcct
    27661 ttgctgatgt tagggtatct tccagcaatc tttaatcaga aaaagcaaca tctaggcgat
    27721 tttttcacta atacaagtgt tgttacgatc aatctgatca aagcattcaa ccaggaagtc
    27781 aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa agtcagatct
    27841 aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt ttttgttttg
    27901 ctactattga acatgagctg aacatgagca agaggaggac actgcgtgaa gccgattgat
    27961 tttccagaaa aatatgagaa tttaatgcga gtagcacaac aagcactagc aaaccagcaa
    28021 tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag ctttgaggct
    28081 aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc attgaagcaa
    28141 gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt ttattttgac
    28201 ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag tacagatttt
    28261 tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga attatccgga
    28321 caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact tccttcgttg
    28381 gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta tcatgagttt
    28441 atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc aagggcaaaa
    28501 ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt gacgattgaa
    28561 gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca ggcaagttac
    28621 cagcaattat gtgtattttc agatcgttac ggtaatgaag atgcgctgct tagttccgtg
    28681 ttaaatgaag aattcacgtt gcaatctgca attgtttatc ctgtctatga tacatacatc
    28741 gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa aacgatgtat
    28801 gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat tttgcagcaa
    28861 atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg aaaaacaagg
    28921 attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt aaaggtatgc
    28981 atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta aatgactgca
    29041 acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc gcaagacgaa
    29101 atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa cgtacctgga
    29161 ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga agaagcttta
    29221 tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt caaagaagct
    29281 ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa aggtcaagac
    29341 tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca atataaagat
    29401 ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc acgtatcaaa
    29461 cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc tgaaaatggc
    29521 gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga tggcggaaaa
    29581 ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt tgaagatcaa
    29641 ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc agaagattat
    29701 caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca tgaagtaaaa
    29761 gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga cgaagtatca
    29821 acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa agaaaaagca
    29881 gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc agaaatcgtt
    29941 gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga attcttgaac
    30001 aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg ttctacagaa
    30061 gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac aaatcttgtg
    30121 atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat caatgcagaa
    30181 atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg cgtgttgaca
    30241 gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat cacaggtaca
    30301 gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa gctacttcta
    30361 gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt ccttattttt
    30421 tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa cactttttct
    30481 ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt ttcttgcata
    30541 ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa tgagaccgta
    30601 cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc tggtccaggg
    30661 gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga agaattttat
    30721 gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct tgaagtattg
    30781 aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc cgtatacaat
    30841 cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga actgcaaaaa
    30901 agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc acaaacatta
    30961 gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac agaagctggt
    31021 tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc tgattacaat
    31081 gtggaacgtg cagaaaaagg gatcatctat attgatgaga tcgataaaat tgcgcgtaaa
    31141 agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca agccttgctg
    31201 aaaattctag aaggaactgt tgcaagcgtg ccacctcagg gaggacgtaa acatccacat
    31261 caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg tgcatttgat
    31321 ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt tggtacacaa
    31381 aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga agatttacta
    31441 aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc tttagagaaa
    31501 ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct agtcaaacaa
    31561 tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga agcgttgaga
    31621 gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg ttccatcatt
    31681 gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat cgaaaaagtg
    31741 attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta taaagattca
    31801 gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc ttttgcgaca
    31861 gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc atcaagctga
    31921 aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc cagaaatagc
    31981 tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga ttgatcgaaa
    32041 gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt tttatttgat
    32101 cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag tatccaaaac
    32161 tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag accagctacg
    32221 tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata tccagatgta
    32281 tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg cagataagat
    32341 ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg attttgatcg
    32401 caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag aagcgtgggc
    32461 agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca gtcaaactct
    32521 ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg tttcttgtct
    32581 tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc ttttggtgca
    32641 actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt atctgtacgt
    32701 tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc tatcatgtaa
    32761 tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcgtt ggttataatt
    32821 aaattataaa aagattttaa agaggtgaga aaatgattgg cgaagataaa ttattgatga
    32881 ctatccagct cacagactta aatgaagaag aaaaaaaaca ggaaataagc gattgggcta
    32941 ttttgacacg tctgttgatc cagccaacat ttatccgctc tttcacacaa caagcggatc
    33001 cgtatgatct tcgaaagctt caagaaaaaa taatgctagc atcggtaaga gacgaaagtt
    33061 ggttagttgt aggaaatgac gagaacgaat gtagttttcg cttggaagat aatcaattat
    33121 tgataaaaaa tgtattgtct atctctgttt tcaaagaaaa gcaatttttg attcgagact
    33181 tcattcagaa aaaaatgatt gctcatgggg tgttcgccta tatgcgagct tatagcgagt
    33241 ttatctatca caatacaaaa cagatctctc aacggctatt gtttgagaaa aaggatgaaa
    33301 tcgaacattt acctaaaatg aaacaacaaa acggggaagt agtagtcgat tgcaaccaat
    33361 ttccaggata cgatcttttt taccaaggtt tatgtttcac ttcgtgttgg gaaatgtatt
    33421 atagtcgcta ttatcatcaa atcatcccta aaccgatttt tctcgacatc caacaagtag
    33481 aaaaagtgaa ggaattagaa aacgaagtca tatgtatcca gctttatcga gaccccttta
    33541 actggcaaaa gccaaacaat caaatgtttc aatcctattt tagggaccag cttgggtttg
    33601 atcatcttgc ttgggacaat ggtgtcggtt tattgaaacc gccattcgtt gaatacattt
    33661 acactgacca tacgatccaa tctgtccagt atcagaatgc acaaatgcaa cctgttccta
    33721 aaaagaatgc ttcatttttt gtaacaaaaa gttatgatat tcaacaagga gattataaag
    33781 aaaggcgtgt tcgcggaaca ctaaatgctc aggcgtattt tccttgggtt gacgagaatc
    33841 gctcgcgcat gatgtgctat aaagttatcg atccgactgt tgctttggat aatggaatcg
    33901 aagcttattg ttattatatt agggaatatc tagaagtaga ggtaacagat gaaaaatatc
    33961 aagagtatct tttatcctta agaatctatg ttccttcaga atccctgtca gaattaccgt
    34021 taaaggaaat caaacataga ctctctgatg ttaccttcaa acgaataaag aaaaagcggc
    34081 gaagtatcca attcgatgtc aagaaaggtg agcagcatct gcgtgtgcaa tttttagatt
    34141 accgagaatt agaacaattg attactttac aaaaaatatg aggtgaagaa aatggataaa
    34201 caactctatt atcgaataat ggttccggca tcattattat ccgtctgtct cttttttgtt
    34261 cctaattttc ctattcgatc agtcatcggt tttttggtca tgtttggcgg gatgggactt
    34321 tattattatc ttcttcataa
//
LOCUS       DAYYGY010000019        33673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000019 DAYYGY010000000
VERSION     DAYYGY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.019"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACHA52_000978"
     CDS             214..498
                     /locus_tag="ACHA52_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8939721.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="ACHA52_000979"
     CDS             complement(815..1198)
                     /locus_tag="ACHA52_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8939722.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="ACHA52_000980"
     CDS             complement(1200..1784)
                     /locus_tag="ACHA52_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8939723.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACHA52_000981"
     CDS             complement(1831..3306)
                     /locus_tag="ACHA52_000981"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8939724.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACHA52_000982"
     CDS             3487..3798
                     /locus_tag="ACHA52_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939725.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACHA52_000983"
     CDS             complement(3913..6249)
                     /locus_tag="ACHA52_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8939726.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACHA52_000984"
     CDS             6391..7059
                     /locus_tag="ACHA52_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8939727.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACHA52_000985"
     CDS             7062..8105
                     /locus_tag="ACHA52_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8939728.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACHA52_000986"
     CDS             complement(8342..9223)
                     /locus_tag="ACHA52_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8939729.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACHA52_000987"
     CDS             9382..10110
                     /locus_tag="ACHA52_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8939730.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACHA52_000988"
     CDS             10266..10583
                     /locus_tag="ACHA52_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8939731.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACHA52_000989"
     CDS             10576..11184
                     /locus_tag="ACHA52_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8939732.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACHA52_000990"
     CDS             11239..12030
                     /locus_tag="ACHA52_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8939733.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACHA52_000991"
     CDS             12168..12656
                     /locus_tag="ACHA52_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8939734.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACHA52_000992"
     CDS             12707..13579
                     /locus_tag="ACHA52_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8939735.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACHA52_000993"
     CDS             13755..14117
                     /locus_tag="ACHA52_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8939736.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACHA52_000994"
     CDS             14126..14878
                     /locus_tag="ACHA52_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939737.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACHA52_000995"
     CDS             14892..16694
                     /locus_tag="ACHA52_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939738.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACHA52_000996"
     CDS             16971..18011
                     /locus_tag="ACHA52_000996"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8939739.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ACHA52_000997"
     CDS             complement(18063..20207)
                     /locus_tag="ACHA52_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8939740.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="ACHA52_000998"
     CDS             complement(20564..21937)
                     /locus_tag="ACHA52_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8939741.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACHA52_000999"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACHA52_000999"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8939742.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACHA52_001000"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACHA52_001000"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8939743.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="ACHA52_001001"
     CDS             complement(23385..24398)
                     /locus_tag="ACHA52_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8939744.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="ACHA52_001002"
     CDS             complement(24479..25078)
                     /locus_tag="ACHA52_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8939745.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="ACHA52_001003"
     CDS             25264..25965
                     /locus_tag="ACHA52_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939746.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="ACHA52_001004"
     CDS             25975..28686
                     /locus_tag="ACHA52_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8939747.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="ACHA52_001005"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="ACHA52_001005"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8939748.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="ACHA52_001006"
     CDS             complement(30825..32006)
                     /locus_tag="ACHA52_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8939749.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..>33673
                     /locus_tag="ACHA52_001007"
     CDS             32258..>33673
                     /locus_tag="ACHA52_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8939750.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYA"
BASE COUNT        10659 a         6038 c         6849 g        10127 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaccttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gca
//
LOCUS       DAYYGY010000020        33287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000020 DAYYGY010000000
VERSION     DAYYGY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.020"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..408
                     /locus_tag="ACHA52_001008"
     CDS             133..408
                     /locus_tag="ACHA52_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8939751.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            414..1181
                     /locus_tag="ACHA52_001009"
     CDS             414..1181
                     /locus_tag="ACHA52_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8939752.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1349..3178
                     /gene="typA"
                     /locus_tag="ACHA52_001010"
     CDS             1349..3178
                     /gene="typA"
                     /locus_tag="ACHA52_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8939753.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3439..5211
                     /gene="recQ"
                     /locus_tag="ACHA52_001011"
     CDS             3439..5211
                     /gene="recQ"
                     /locus_tag="ACHA52_001011"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8939754.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5325..5627
                     /locus_tag="ACHA52_001012"
     CDS             5325..5627
                     /locus_tag="ACHA52_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8939755.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5630..6793
                     /locus_tag="ACHA52_001013"
     CDS             5630..6793
                     /locus_tag="ACHA52_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8939756.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6819..10247
                     /locus_tag="ACHA52_001014"
     CDS             6819..10247
                     /locus_tag="ACHA52_001014"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8939757.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10268..11392
                     /locus_tag="ACHA52_001015"
     CDS             10268..11392
                     /locus_tag="ACHA52_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8939758.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11373..11819
                     /locus_tag="ACHA52_001016"
     CDS             11373..11819
                     /locus_tag="ACHA52_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8939759.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11803..12141
                     /locus_tag="ACHA52_001017"
     CDS             11803..12141
                     /locus_tag="ACHA52_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8939760.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12260..12820
                     /gene="rsmD"
                     /locus_tag="ACHA52_001018"
     CDS             12260..12820
                     /gene="rsmD"
                     /locus_tag="ACHA52_001018"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8939761.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12817..13308
                     /gene="coaD"
                     /locus_tag="ACHA52_001019"
     CDS             12817..13308
                     /gene="coaD"
                     /locus_tag="ACHA52_001019"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8939762.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13298..14341
                     /locus_tag="ACHA52_001020"
     CDS             13298..14341
                     /locus_tag="ACHA52_001020"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8939763.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14500..15228
                     /locus_tag="ACHA52_001021"
     CDS             14500..15228
                     /locus_tag="ACHA52_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8939764.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15344..15589
                     /locus_tag="ACHA52_001022"
     CDS             15344..15589
                     /locus_tag="ACHA52_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8939765.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15586..15804
                     /locus_tag="ACHA52_001023"
     CDS             15586..15804
                     /locus_tag="ACHA52_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8939766.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15940..16479
                     /locus_tag="ACHA52_001024"
     CDS             15940..16479
                     /locus_tag="ACHA52_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8939767.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16472..18745
                     /locus_tag="ACHA52_001025"
     CDS             16472..18745
                     /locus_tag="ACHA52_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8939768.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18783..19829
                     /gene="holA"
                     /locus_tag="ACHA52_001026"
     CDS             18783..19829
                     /gene="holA"
                     /locus_tag="ACHA52_001026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8939769.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19918..20172)
                     /gene="rpsT"
                     /locus_tag="ACHA52_001027"
     CDS             complement(19918..20172)
                     /gene="rpsT"
                     /locus_tag="ACHA52_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8939770.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20572..20841)
                     /locus_tag="ACHA52_001028"
     CDS             complement(20572..20841)
                     /locus_tag="ACHA52_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939771.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21103..21231)
                     /locus_tag="ACHA52_001029"
     CDS             complement(21103..21231)
                     /locus_tag="ACHA52_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939772.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(21311..21766)
                     /locus_tag="ACHA52_001030"
     CDS             complement(21311..21766)
                     /locus_tag="ACHA52_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8939773.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21904..23832)
                     /locus_tag="ACHA52_001031"
     CDS             complement(21904..23832)
                     /locus_tag="ACHA52_001031"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8939774.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24029..24862)
                     /locus_tag="ACHA52_001032"
     CDS             complement(24029..24862)
                     /locus_tag="ACHA52_001032"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8939775.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25054..25758)
                     /locus_tag="ACHA52_001033"
     CDS             complement(25054..25758)
                     /locus_tag="ACHA52_001033"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8939776.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25781..26407)
                     /locus_tag="ACHA52_001034"
     CDS             complement(25781..26407)
                     /locus_tag="ACHA52_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8939777.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26733..29108
                     /locus_tag="ACHA52_001035"
     CDS             26733..29108
                     /locus_tag="ACHA52_001035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8939778.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGVYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29132..29623)
                     /locus_tag="ACHA52_001036"
     CDS             complement(29132..29623)
                     /locus_tag="ACHA52_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8939779.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29810..30730
                     /locus_tag="ACHA52_001037"
     CDS             29810..30730
                     /locus_tag="ACHA52_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8939780.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30884..31228
                     /locus_tag="ACHA52_001038"
     CDS             30884..31228
                     /locus_tag="ACHA52_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8939781.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31418..31918)
                     /locus_tag="ACHA52_001039"
     CDS             complement(31418..31918)
                     /locus_tag="ACHA52_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8939782.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32446..32529
                     /locus_tag="ACHA52_001040"
     tRNA            32446..32529
                     /locus_tag="ACHA52_001040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32480..32482,aa:Leu,seq:cag)
     gene            complement(32853..>33287)
                     /locus_tag="ACHA52_001041"
     CDS             complement(32853..>33287)
                     /locus_tag="ACHA52_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8939783.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11006 a         5772 c         6785 g         9724 t
ORIGIN      
        1 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
       61 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
      121 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
      181 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
      241 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
      301 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
      361 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
      421 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
      481 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
      541 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
      601 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
      661 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
      721 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
      781 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
      841 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
      901 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
      961 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
     1021 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
     1081 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
     1141 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
     1201 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
     1261 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
     1321 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
     1381 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
     1441 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
     1501 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
     1561 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
     1621 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
     1681 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
     1741 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
     1801 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
     1861 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
     1921 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
     1981 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
     2041 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
     2101 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
     2161 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
     2221 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
     2281 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
     2341 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
     2401 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
     2461 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
     2521 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
     2581 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
     2641 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
     2701 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
     2761 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
     2821 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
     2881 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
     2941 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
     3001 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
     3061 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
     3121 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
     3181 tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt
     3241 ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc
     3301 gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa
     3361 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
     3421 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggcta tgatgagttt
     3481 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
     3541 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
     3601 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
     3661 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
     3721 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
     3781 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
     3841 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
     3901 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
     3961 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
     4021 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
     4081 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
     4141 agaaaagaag tagaccgtct ctatcatttg cttagaaaat tcgatttttc cgtgggtaga
     4201 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
     4261 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
     4321 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
     4381 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
     4441 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
     4501 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
     4561 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
     4621 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
     4681 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
     4741 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
     4801 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
     4861 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
     4921 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
     4981 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
     5041 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
     5101 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
     5161 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
     5221 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
     5281 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
     5341 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
     5401 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
     5461 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
     5521 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
     5581 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
     5641 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
     5701 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
     5761 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
     5821 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
     5881 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
     5941 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
     6001 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
     6061 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
     6121 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
     6181 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
     6241 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
     6301 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
     6361 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
     6421 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
     6481 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
     6541 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
     6601 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag ggattactgg
     6661 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
     6721 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
     6781 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
     6841 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
     6901 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
     6961 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
     7021 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
     7081 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
     7141 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
     7201 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
     7261 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
     7321 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
     7381 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
     7441 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
     7501 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
     7561 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
     7621 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
     7681 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
     7741 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
     7801 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
     7861 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
     7921 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
     7981 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
     8041 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
     8101 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
     8161 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
     8221 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
     8281 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
     8341 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
     8401 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
     8461 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
     8521 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
     8581 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
     8641 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
     8701 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
     8761 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
     8821 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
     8881 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
     8941 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
     9001 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
     9061 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
     9121 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
     9181 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
     9241 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
     9301 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
     9361 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
     9421 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
     9481 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
     9541 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
     9601 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
     9661 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
     9721 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
     9781 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
     9841 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
     9901 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
     9961 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    10021 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    10081 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    10141 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    10201 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    10261 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    10321 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    10381 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    10441 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    10501 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    10561 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    10621 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    10681 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    10741 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    10801 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    10861 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    10921 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    10981 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    11041 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    11101 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    11161 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    11221 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    11281 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    11341 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    11401 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    11461 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    11521 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    11581 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    11641 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    11701 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    11761 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    11821 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    11881 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    11941 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    12001 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    12061 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    12121 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    12181 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    12241 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    12301 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    12361 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    12421 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    12481 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    12541 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    12601 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    12661 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    12721 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    12781 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    12841 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    12901 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    12961 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    13021 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    13081 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    13141 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    13201 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    13261 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    13321 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    13381 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    13441 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    13501 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    13561 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    13621 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    13681 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    13741 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    13801 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    13861 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    13921 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    13981 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    14041 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    14101 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    14161 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    14221 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    14281 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    14341 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    14401 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    14461 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    14521 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    14581 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    14641 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    14701 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    14761 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    14821 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    14881 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    14941 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    15001 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    15061 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    15121 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    15181 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    15241 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    15301 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    15361 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    15421 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    15481 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    15541 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    15601 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    15661 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    15721 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    15781 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    15841 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    15901 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    15961 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    16021 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    16081 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    16141 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    16201 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    16261 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    16321 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    16381 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    16441 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    16501 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    16561 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    16621 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    16681 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    16741 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    16801 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    16861 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    16921 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    16981 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    17041 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    17101 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    17161 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    17221 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    17281 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    17341 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    17401 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    17461 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    17521 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    17581 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    17641 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    17701 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    17761 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    17821 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    17881 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    17941 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    18001 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    18061 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    18121 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    18181 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    18241 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    18301 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    18361 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    18421 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    18481 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    18541 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    18601 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    18661 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    18721 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    18781 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    18841 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    18901 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    18961 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    19021 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    19081 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    19141 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    19201 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    19261 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    19321 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    19381 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    19441 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    19501 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    19561 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    19621 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    19681 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    19741 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    19801 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    19861 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    19921 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    19981 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    20041 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    20101 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    20161 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    20221 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    20281 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    20341 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
    20401 tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga
    20461 gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc
    20521 tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg
    20581 tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa
    20641 ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg
    20701 taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag
    20761 ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa
    20821 aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg
    20881 cactaattct tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca
    20941 ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt
    21001 ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc
    21061 ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa
    21121 cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc
    21181 tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc
    21241 tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac
    21301 aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag
    21361 caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa
    21421 caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga
    21481 cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta
    21541 atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga
    21601 tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg
    21661 gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta
    21721 aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc
    21781 actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct
    21841 tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt
    21901 tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga
    21961 agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat
    22021 tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga
    22081 agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg
    22141 catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac
    22201 gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc
    22261 ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag
    22321 ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg
    22381 ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat
    22441 acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg
    22501 aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt
    22561 tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt
    22621 ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa
    22681 tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc
    22741 gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag
    22801 aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc
    22861 tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa
    22921 atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga
    22981 agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc
    23041 tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag
    23101 aggaacagca agcaaaatca attttacata attattttcg attcgttcga taaatgaagc
    23161 tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt
    23221 ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc
    23281 atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc
    23341 atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc
    23401 gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt
    23461 ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg
    23521 acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat
    23581 tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa
    23641 atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata
    23701 tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc
    23761 gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt
    23821 ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta
    23881 attgataata ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg
    23941 atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc
    24001 tcaaataaga gaagtgttct ggttttttct acattgcaat aaaccaataa ccaatcaaaa
    24061 tgataccaag gataatacga taccagccaa aagcggtaaa gtcgtttttc ttcaaatagc
    24121 ttagtaagaa tttgatagct aagacggaaa ccacaaaaga tacgaatgtt cctacaagaa
    24181 gaacaacagt ttcttccatt ttgaatgaat tcccttcagc taaatatttg acgatcttca
    24241 agccgcttgc gccaaacatg acaggaatcc ctaagaaaaa tgaaaactct gtagcgacaa
    24301 aacgcgaagc accaatcaag atagatccta ggatcgttgc gccagaacgg gatgttccgg
    24361 gaacaagtgc caagacttgg aataacccaa caagaagagc tgcttggtac gtaaagtcat
    24421 tcagagaagt ccaacgaggt gttttgtttt tgttgcgttt ttcgacaata ataaaggcaa
    24481 taccgtaaac gatcaaaaca atggcaactg gtaagaactt gtggaaattt gcttctaacc
    24541 aatcatccaa aggcaaacca atgatgatgg ctggtatgca agcaacgatc actttaaacc
    24601 aaagtgtcca cgtatctttt ttttctaccg ttgatttact tggagaaaat gggtttaact
    24661 tgtgaaaata aagaacgacg acagccaaga tcgcccctaa ttggataacg acattgaaca
    24721 tcgacataaa ttgtgaactg gcatccagtt tgatgaattc atctgctagg atcaaatgac
    24781 cagtactact gatgggcacc cattccgtta ttccttcaat aataccgaga atcactgctt
    24841 tgataatatt tgcaaaaaac ataaattcct tccttcccaa ttaagataca tcttatagta
    24901 taccgttatt ttttgagaaa cccaatctgt tttttaatat taagaaaaac ttgagaaata
    24961 aagcagataa gtacacgaag agaaaaaaga agacaaagcc ctcgttcaga ctttgccttt
    25021 tatgattctc tggtaaaaat taaaatatcc ccattataga aacgagtcgc gactatgtgt
    25081 tttcccgtta tcgatataag acgtatcatt gatgccgtgg atagaaaatc gaccgtttgc
    25141 ataagtgacc tttgttacgc tgccatttaa aagcccagca cgaacaggct gtttaggatc
    25201 gatcagatta agcaaaaagg cgatcgtcaa accatgagat acgactaaaa catttccacc
    25261 accatttttt tcgcagtgat gggcgatatc ttcaaatcct gtccatacac ggtctcgcaa
    25321 tgtttcataa gtttccgccc acccagcggt atctaagttg tatacagcat tagcaatttg
    25381 ttcaaaagaa acttctgtgg taaacatttc atcatatgtt ttaaaattca atacacgagg
    25441 gatcacaccc cacatttcca tgtcatatcc accttcaaga gacccgaaac accattctct
    25501 aatccttttt tcaataaaat aaggaatttc ttttccctta gaatgttctc ctagaataat
    25561 acgggcagtt tcgattgcac gaccactgtc actagaataa gcaaatgaaa agtcgacatt
    25621 ttttaacccg cgacctaaat aatgaatgcc ttcttgtcct tgctttgtga ggggtgtatc
    25681 acaccagcct tgtactcgtt caattgtatt gaacatcgtt tttccgtgtc gtacgatata
    25741 aagtgtagtt tctgtcattt tattctcctc ctaagtcgtt ttaacgattg aaaaaaggat
    25801 ttgtcctttt ttcctgttca atcgtcgttg catcgccatg tcctggatag actgcaaatt
    25861 catcaggtaa tacaaacagc tgggtccgaa tactatgaag taattgttct aaattccctg
    25921 tatacagatc ggttctaccg atacttcctc taaacagggc gtcgccagaa acgacaaaat
    25981 catcaaaaat aaagctgacg cttcctatag aatgccctgg ggtagggacg acgcgaaaag
    26041 acatgtttcc taaagtataa gttttcattt caaattcata ttctgcaggc gagacaataa
    26101 catcagcaat atcatcatgt ctaccgagtc cagaaagatt caaaatcgga tcacctagcc
    26161 atttttgctc tagtgggctg acataaactg ggatttgata atacgcgcgt aaaggttcca
    26221 ctgcaccgat atgatcgtag tgagtatggg taagcaaaat cgctaccggc tgttggtttg
    26281 tttttttgat ttcttgtttg attcgttccg catcttctcc agggtcaatg atcaaaagtg
    26341 cttcttcgtt gtagaccaaa taacagtttt cttcaatcat tcctgttttt aatctttcga
    26401 tatgcatgtg cattcctcct tgatagccaa aacttctttc atccattagt ataacgcatt
    26461 cattgctagg aaacaaaaac aggtacttat ttctctttac ttatctgaat tatcagacta
    26521 tatgaaaagt atttggatat tctaaagaga aggaaagtaa gttttcatag cattttaata
    26581 tgtccttgtt acattagatt catccaaaaa tccaaattct atatcctata tccctgccta
    26641 atcagcaggg tgttttttta tgtttggaaa gagagctctt tttgctaaac taggtttttt
    26701 caggtaatct tagagagaaa gaggtggtgg aaatgatcaa acagtcaaaa atatctgtgc
    26761 ggcacttagt agaatttatt ttgcgacgtg gaagtatcga caatcgtaaa aaaagtaacc
    26821 atactgcatt agaaggagcg agaatccacc gcaagctcca aaaagatgcg ggagaaaact
    26881 atgaaaaaga agtcttttta aaaacaacag ttcaattaga tgaatattac ctgatcgttg
    26941 aaggccgtgc agacggtatt ttcaaaagag acggtgttta ttatatagat gaaatcaaaa
    27001 cctccgaacc acgatttgaa gatttagaac cagaacaagt ggaacttttc tttcatcaag
    27061 caagggtgta tgcctatatc tatagtcaag aacatgattt acaagaaata aatctgcaat
    27121 tgacttactt ccagacggca gaagaagtga tcacgagaaa ggttgaacat cagacaagag
    27181 agcagttaga aacttttttt aaaaagctga ctgaagatta taaagaatgg cttgtttttc
    27241 aagaaaactg gcgcactgta cgcaatgcct ctttgatggc tttgaaattc ccccatgatg
    27301 agtataggaa aggtcagcgt gaactagcgg tagctgctta taaaacgatc cgtacgaaac
    27361 agaaattgtt tgtagaagca cctaccggaa caggaaaaac aatctctacg ttattccccg
    27421 ctttgaaagc tgtaggggaa gaagagggag aaaagatctt ttatctaacg gctaaaacaa
    27481 taactagaca agtagctgaa gatgctatga cagcattaaa agatacagga gctgaagtaa
    27541 aaagtgtcac tttgacggcg aaagacaaga tctgcttttt agatgagacg acctgtaatc
    27601 cagatcagtg cccatatgcc aatggttatt acaatcggat taatgaaggt ttatgggatc
    27661 ttttgaatca tgagaatcag atcactcgtg aagtcatcga aacttatgca agaaagcata
    27721 cactttgtcc tttcgaacta tcattagatg tcagtatctg gtgtgacgta atcattgggg
    27781 attacaatta tttatttgat ccaactgttt atttaagacg tttttttgaa gatgaaaaaa
    27841 acgaggacta tctgtttttg attgacgaag cacataattt ggtgaatcgt tcccgagaga
    27901 tgtattcggc agaactatct tatgaaaaaa cgaaacgaag taaggaagct ataccaaagg
    27961 aattcaaaaa acttcatcgc cgtttcaaca aactattaaa agaatttgat tcgatccgag
    28021 aaatagcaaa agaagatcat tgggattatc atcatcaaaa agcaccggct gaatctttag
    28081 taaaggcagg ttatcaatta agcgaaaaaa ttaaagaatg gctggcagaa tttcctgagc
    28141 acccatcaca agaacaattg cttccttatt attttgattt gcttcatttt ttgaaggtca
    28201 gtgaatttta tgatgatcac tacgaaacaa cagtcgaaaa aacgtatcgc gatctgatag
    28261 taaaagagtt ttgtattgat ccaagcttgt ttcttgagca gtcgttggat aaaggaaaaa
    28321 gcagtattct tttttctgct agtttttcac cactctctta ttatcaggaa acactggggg
    28381 gacaaaaaag tctggcttac aaactgccga gtcctttccc agaaggaaac cagcaagttt
    28441 tcatcgcaaa ttacctagag acgacttatc gaaaaaggga acacagtctg gcaaaattag
    28501 tagagacgat tcatgctttt gctgatggaa aggtcgggaa ttatttcgtt ttctttcctt
    28561 cttatcaata tttagatctt gcggtggaag cattcagaca gcaatatccc gacagcaatg
    28621 tgctaatcca agaaacggac atgaatgagg aagaacgcga acgattttta acaaaatttc
    28681 aaacaaatcc cgctgaaaca ttgattggat tttgcgtact tggcgggatt ttttcagaag
    28741 gaatcgattt gagaggggac cgattgatcg gcagtatgat cgtaggtgta ggtttgccgc
    28801 agatgaatca tgagcaggaa ctgatcaaat catattatga tgagaaggaa caccttggat
    28861 ttgcttacgc ttatcagctt cctggaatga acaaagtctt gcaagctggt ggtcgagtga
    28921 ttcgtggcat ggcggatcaa ggaatcgttg tattggcaga tcaacgattt tctagttttt
    28981 cttatcgacg tttattccca caacattggc aaacagcaag agaagtacga agtgttgggg
    29041 aattggaaga aggaatacag cgtttttggt tatcaaaaga agcagtaaat caaaaaaacg
    29101 aggaatgaca gacgtcagcc tcgttttttg attaacgata gttagtgaat tgcaactcga
    29161 ttggtaagtc aagtttacgt aataaagcaa tcacagcttg tagatcatca cggttcttac
    29221 ctgtcactct aatctgatct tcttggattt gtgcttttac tttcagtttt tcatttttga
    29281 tagctgtagt gattttcttc gcgttttcac gatcgattcc gttgaccaaa tcggcagttt
    29341 gtcgagcttt gccacctaga gcgtgttctg attcggaaaa atggatattt ttgattggca
    29401 catttctttt gatcagctta ccaaataata catctttgat ctgttcgatt ttgaaatcat
    29461 catcgctcaa tacaactaga ttgctgttct ctaatttgat ttctactgta gaatccttga
    29521 agtcaaagcg attggacaat tcttttgttg tttgctgaat cgcatttttt acttcttctg
    29581 tattgacttc tgatacaatg tcgaagctag catcttttgc cataggaacc ctcctgatat
    29641 acatataaat atttaccata catctatctt atcataggat tagggcagat tcttacctgt
    29701 ttgctaaaat tcttctcatt tgagttcgaa gtattttatt ttaatagata aactgttatt
    29761 cttacatttg gaataccaaa gtaggggaga ggtgcttgtg agtaaaatta tgactgcgta
    29821 tcataaaaat gaaagattaa agaaaatagt ggttattttg ataaccggat tgaccgctgc
    29881 ggtcgcttta aattattttt tgattccagc caatgtcttt tctgcaggga tgaatgggat
    29941 cgctcaaatc atttcctcgc ttttatatga atggtttcat atcaagatag acacaggaac
    30001 atttattttt cttttgaata tccctgtgtt tgtattaggg tttctcaaag taggaaaacg
    30061 agcaacgatc cttagttttg tgaatgttgt atgtgtatcg gtgatgacga tgctgcttcc
    30121 agtcggacag atcactgata atattttgat gaatgcccta gtaggcggtg ttttattagg
    30181 tcttggcgta ggattatctt taaaaatggg ctttacaact ggtgggatgg atatcatctc
    30241 tcttgtactt tcacaaacaa ctggaaaaac agtcggaaac tacatgctga ttctaaatgg
    30301 tatcattgta gtcatcgcag gcttcttctt taactgggaa agtgccttgt atacgattat
    30361 ttccatttat gcaatgactc aagtaattga tgccatccac acgagtcatc aaaaagtaac
    30421 agcaatgatc gttaccgtca agccagaaga ggtaacagaa gaactagcaa aacggatggt
    30481 acgtggaatg acattactgc cgtctgtcgg tggctattca aaaaaacaag ggaacatgat
    30541 catgatggtc atcacccgct acgaaatgta tgatcttgat cagatcgttc atgaaatcga
    30601 cgacgatgca tttatcaata ttttaccaac tcattcagta tttggccggt ttgcaaatga
    30661 aaacgaacaa aaaatgtatc gaacaactgg cgtattgcca gaaataaaaa atcgttcaca
    30721 aaaaaaatga ggaatcaggt ttattttaga ataaatttaa tctattacat ctaataagaa
    30781 catttttata aatttaaacg agaatcttac ttctaaattc tcatttacaa gcagctaaaa
    30841 aaagagtatg ataaataaaa atcgtgttaa ggagtgtcgt acgatgagcg aagcaaacca
    30901 agaacggtct ggacaagaaa tagaactaat caaagaacgt attcttgctg ccttggaaat
    30961 ggtgattgat cctgaattag gaatcgacat cgtcaattta ggcttgattt atgatataga
    31021 attcaatcct gaaaatggag aaacagtcat taagatgact ctaacaacta tgggctgtcc
    31081 attagcagat attttaacag aatctatcca tgacgcatta aaagaagtgc cagaagtaac
    31141 gaaagcagaa gtcaaactag tctggtatcc agcatggacg acagataaga tgagccgtta
    31201 tgcacggatt gcgttgggga tacgatagaa atcttggctg actagctatg agaagcaaat
    31261 aggaaaaaca aaaaagcctt ttatcctttc tagaagaaaa gagataaaag gtttctttgt
    31321 ttataaaatt ttgtctgcta tattataagt taattaaata aatgtgttct attgataaag
    31381 gacattcaaa tccctttcct tttggaatcc ttctttttta catcagtttg attttcgtat
    31441 agtattcttt ctcatcttca cgataaatcg cagccccatt tttttgttgt gctttaagac
    31501 tgccaagatt ttttttattt acagacaaaa gtgcctcatc aatgccgatt ttttttgcct
    31561 cttgtagtac taatgctaat ccttgtgtgg catatccttt tttacgataa ttaggggaga
    31621 tgccgtaacc aatatggcca gcgccgtttc ttaatgtttc gttcagttta tggcgcaaat
    31681 taaaaattcc tacatactca tgttcatcac tttctaaaat aaagacggtt gcgggaacgt
    31741 aactctcagg aagatcgatt cctaaagacg ttttttcgca tttttttaca taatctaaaa
    31801 attcagcaaa tgtatagcca taagcattgt tgataaatcc actctcattt ggtccaaagc
    31861 tagtttgtag ctgataagct ttctttaaat ctgcttccca tagtttgatc agtttcatcg
    31921 tattcacctc tttaagtaac cttattcatt tgttttctac tatagcacta ttgttgtaaa
    31981 tagccatgcc tatttttatt tattgtgtca tatcgtatta agaatcatcg aacaatacaa
    32041 atatgaatta caataaaaaa attatttgaa gttcgttcat tcgttactta tgatctctag
    32101 aagaaagaaa acgtgaatta cttcagcgtc tagaattcca gaaggatagt aatcaacaga
    32161 agaaataatc aatatccaaa tgaaagaaga tagctgaatc gagaaaaaga ggaaaaaaga
    32221 aatgaagaaa aaaataaaat agttactaat cccagagtcg ttcgtttaac attgaaatcg
    32281 atcatatgga taaaacagat tttttcacca accctcaata gatgatggag ttgctcggat
    32341 gaattatttg tgtagatggc ataagggagg gcgtacagaa atgaaaaaga acaaaaatat
    32401 gttcttgaca aaaaacacat gtaatcctaa actaaaagat gtgatgcggc tatggcggaa
    32461 ttggcagacg cgctagcttc aggcgctagt gagggtgacc tcgtggaagt tcgagtcttc
    32521 ttagccgcat aatttgtttt tttataaaga gaaggtacac ctggagcgat gagaaagaaa
    32581 ataacatatt atctaaaaga atataggaaa aattttaaac aacggaacca gattcaaata
    32641 aatcaaaaaa aatttgtgtg aaggtgggac agtttaaaac ctaatagatt gattcaaata
    32701 gtagatttat agattctgat acaagaaata ctggcaaacg atagttaagg aataaagact
    32761 aaacttaaac tcaaaaagag gtcatgcgaa agaataaatc gcatgacctc ttactatatt
    32821 ttgaatgtgt ttaaacgttc aaataatgaa tcttagtacc aaccgtttgc ttcccagaat
    32881 gctttcgccg cttcccaaga accataacgt gaagtaacat attgttctgc tactctttct
    32941 tggtttgcag cagagtagtc accatttaag taagaagaat ctaattggta acggccgatg
    33001 taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat ccattctttt
    33061 gcagagcttg tacttgctgg agtagcagca gtttctgtta cagcaggagc aggttgtgtt
    33121 tcagcaacag gcgctgcttg tgtttccgtt tgaaccgttt cttgtacaac aggttgttca
    33181 gcttgaacag gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    33241 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DAYYGY010000021        32736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000021 DAYYGY010000000
VERSION     DAYYGY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.021"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            308..628
                     /locus_tag="ACHA52_001042"
     CDS             308..628
                     /locus_tag="ACHA52_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8939784.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            789..1694
                     /locus_tag="ACHA52_001043"
     CDS             789..1694
                     /locus_tag="ACHA52_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939785.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1691..2464
                     /locus_tag="ACHA52_001044"
     CDS             1691..2464
                     /locus_tag="ACHA52_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939786.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2641..4065)
                     /locus_tag="ACHA52_001045"
     CDS             complement(2641..4065)
                     /locus_tag="ACHA52_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8939787.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4276..4722
                     /gene="nrdI"
                     /locus_tag="ACHA52_001046"
     CDS             4276..4722
                     /gene="nrdI"
                     /locus_tag="ACHA52_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8939788.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(4792..6774)
                     /locus_tag="ACHA52_001047"
     CDS             complement(4792..6774)
                     /locus_tag="ACHA52_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8939789.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6788..7684)
                     /gene="murQ"
                     /locus_tag="ACHA52_001048"
     CDS             complement(6788..7684)
                     /gene="murQ"
                     /locus_tag="ACHA52_001048"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8939790.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(7687..8748)
                     /locus_tag="ACHA52_001049"
     CDS             complement(7687..8748)
                     /locus_tag="ACHA52_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8939791.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8960..9487)
                     /locus_tag="ACHA52_001050"
     CDS             complement(8960..9487)
                     /locus_tag="ACHA52_001050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8939792.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(9554..11056)
                     /gene="eat(A)"
                     /locus_tag="ACHA52_001051"
     CDS             complement(9554..11056)
                     /gene="eat(A)"
                     /locus_tag="ACHA52_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8939793.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(11314..12435)
                     /locus_tag="ACHA52_001052"
     CDS             complement(11314..12435)
                     /locus_tag="ACHA52_001052"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8939794.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            12561..13502
                     /locus_tag="ACHA52_001053"
     CDS             12561..13502
                     /locus_tag="ACHA52_001053"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8939795.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13645..14541)
                     /locus_tag="ACHA52_001054"
     CDS             complement(13645..14541)
                     /locus_tag="ACHA52_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8939796.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            14823..16163
                     /locus_tag="ACHA52_001055"
     CDS             14823..16163
                     /locus_tag="ACHA52_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8939797.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16358..16801
                     /locus_tag="ACHA52_001056"
     CDS             16358..16801
                     /locus_tag="ACHA52_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8939798.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17063..18481)
                     /gene="nhaC"
                     /locus_tag="ACHA52_001057"
     CDS             complement(17063..18481)
                     /gene="nhaC"
                     /locus_tag="ACHA52_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8939799.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(18885..20108)
                     /locus_tag="ACHA52_001058"
     CDS             complement(18885..20108)
                     /locus_tag="ACHA52_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8939800.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20140..20979)
                     /locus_tag="ACHA52_001059"
     CDS             complement(20140..20979)
                     /locus_tag="ACHA52_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8939801.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(20996..21676)
                     /locus_tag="ACHA52_001060"
     CDS             complement(20996..21676)
                     /locus_tag="ACHA52_001060"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8939802.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(21678..22751)
                     /locus_tag="ACHA52_001061"
     CDS             complement(21678..22751)
                     /locus_tag="ACHA52_001061"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8939803.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(22810..23048)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23091..23324)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23401..24072)
                     /locus_tag="ACHA52_001062"
     CDS             complement(23401..24072)
                     /locus_tag="ACHA52_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8939804.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KKWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(24160..26808)
                     /gene="mgtA"
                     /locus_tag="ACHA52_001063"
     CDS             complement(24160..26808)
                     /gene="mgtA"
                     /locus_tag="ACHA52_001063"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8939805.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKTIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWF
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27151..27325)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27498..27935
                     /gene="ndk"
                     /locus_tag="ACHA52_001064"
     CDS             27498..27935
                     /gene="ndk"
                     /locus_tag="ACHA52_001064"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8939806.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(27957..28823)
                     /locus_tag="ACHA52_001065"
     CDS             complement(27957..28823)
                     /locus_tag="ACHA52_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939807.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(29057..31135)
                     /locus_tag="ACHA52_001066"
     CDS             complement(29057..31135)
                     /locus_tag="ACHA52_001066"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8939808.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            31628..>32736
                     /locus_tag="ACHA52_001067"
     CDS             31628..>32736
                     /locus_tag="ACHA52_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8939809.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         9533 a         6895 c         5807 g        10501 t
ORIGIN      
        1 ttgcctgcta atatataaaa ataaggtcta gttactgttt ttttaaatga ttaataaata
       61 acaactcctt tctatgaaat tgcaaagcat tcatcttctt ttctgctaac gatatatccg
      121 tcaatgcttg ttacacttta ctaaaaagta tgagattgcc gatatctgga taggtgtgct
      181 atactacaga aaaagatata cgatctgcca ccgggtagta aaaaagagga tgatcattcc
      241 attaggtctt tgaagggatg attctcctct ttttgtgtat tttttatatt gaaggaggaa
      301 cgtatgtatg gcatggatgg aattaatcat agcaggaatt ttggaagtat tctggtcaac
      361 gatgatgaaa tggagtgatg gtttttctaa aatcaattat tctttttaca cagtgattgg
      421 aatgattgct agtttttact ttttgtcaaa ggcaatcaaa agtttgccta tgagtctctc
      481 ttatcctatt tggacaggga ttggcgcagt tggttcggtc attattggtg tggtcttgtt
      541 tcatgataag ctgaatcttt ccacttggtt ctttgtcggg ttgctgctca tcagcatcat
      601 tggcatcaaa atcactagcg gtcactagat acttagacaa gcagacaagg actgatcaga
      661 aaaagcgggg ctttttattt gatattcctc tttctatgaa aaaacaagaa aataagacta
      721 atgacctagc gacttctcta ctcatcaggt gtatgcttgt actatctata gaaaaggagt
      781 gaatattttt gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag
      841 ccctcagcga tgtcagtttt agcttatctc ctggcaaaat cgttggatta gtaggtccta
      901 atggcgctgg aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagaag
      961 gaacgatccg tattggtgaa aatgagattt ctccctctag tcataaagga ttagaacaag
     1021 taggagcatt gatcgaatat ccgggtatct atccattctt aacaggttat gatcatctga
     1081 agttattctc tgaaacaaac gatgtatctg ctatcgacac tatcgtgaaa cagctcaaga
     1141 tggaaaaata cattcacaaa aaagcgaaat cctactcgct gggaatgaaa caaaaattgg
     1201 gaattgctct tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg
     1261 gtttggatcc acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg
     1321 gcacctcttt cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc
     1381 tgatcctgat caatcaagga aaaatcatca aacaatgtac aatggctgaa ttgaaccaaa
     1441 cagcgcaaga tttcatcgtt atctctacga caaatgatca agctgctcga acgatcttga
     1501 cagacgctgg atatcccttg gaagatcctg ataaaatcaa gcttctaaaa aaaaacgaag
     1561 agttattagg aactgttatc cagttattgt ctgcagaaaa tgtccatgtg acagatgtcc
     1621 agcatctgac gaatgatttg gaatcttccc tattggaaat tctggattca gtaaaggggg
     1681 aagaagaaaa atgacgttgc ttataaaaca agaattattt aaattgatca agaaaaagtc
     1741 aactgctgtt ttatctgttc tattagttgc tttgctcata ggaacagcac ttctagccaa
     1801 aaaatacacg acgatcattg atccagttga aatgactgct cagcttttca gtgccacatc
     1861 ttggattgtc tttatcatga ttgctgctgc aagtacgatc atttcaatgg aatcacaata
     1921 tggtacattg aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagtaa
     1981 atggatcaca ttagttattt attctattta tttatacctt ttagctatta ttgtcaccgt
     2041 gctgatgaaa ctgatccttt tcccttcgat ttcctttacg gagaagattt ctactggtca
     2101 gacactgatc caaagcttat tcacttatac tttgggtagt tatatcggtt tatggctaat
     2161 tttaagttta gtgttgatga ttgcttgttt tatcaatagt tccggtgcct ccatctcagc
     2221 agggatcgtc ttttattttg cttcatcaat catatctggt atcctcatcg cactcattca
     2281 aaaatgggag tggatcaaat ggaatccaat cagtatgctg aatctgcaaa accaaattgg
     2341 caacgaagag attatgaagc aattgactca tctttctact aatcaaatgc tgtttggcaa
     2401 tttagcttac attgctttgt tcttagcatt gggatacctc gtattcaagc gaaaaaatgt
     2461 atgatggttc attaacaaag aagttgtgac agaagtggcc agtttcaaga aatttcggct
     2521 tatttttaga agaagctact tcttgaacac catttattca aaaaaagaag cgatgacacg
     2581 aatgttgtca ccgcttcttt tttgaataaa ccacaggctt cacagcctct ctttatactt
     2641 ctatacgtga agcatcccag tggcaaataa aaccattgcg attactccga ccatcgtcac
     2701 accgataagt aaaatcttat ctaagtggat atgttgtaca ggttcgtttt gttctttacg
     2761 tgctttgagg taaaaataaa tacctggtaa gaaaagaaca agaacaagca tcacttcatt
     2821 aaaggctaca actagcatgg cccatacttg aaaagcactt gctaaaaacc ctactaaaaa
     2881 ttggacccac tctttccgct gataagaaag cttcatttgg tacagcccta agaaaagcca
     2941 cgtgaagaaa atcgctgctg taccaagtgt ataagcaaag ttatatgcac tgtcagtgaa
     3001 taataatgaa agtaaaaaga cattcgtaca taacgtcgtc aatacgatag cataagttgg
     3061 tgccccattt ttgttgagtt ttccccatgc tttaggcaaa agtccgtcct cagccatatt
     3121 ccccattgtt tcaatcggca agattgtcca tgagagccaa accccaacta aagaaatgat
     3181 caaaccgccg ttgatcacga ccgcccccca tttaccaacc atcatttcaa atacatatcc
     3241 catagcaggt tgcgctttga tcgtttgcag ctgattcatc gtcattgtgc catatggaag
     3301 catggaaacc aagacgtaaa taatgatcaa accgaatatt cctagaatcg ttgctttccc
     3361 aacatccgat tgtttttttg ctcgtgaacc tacgacagaa gctccttcaa taccaacgaa
     3421 tacccagagc atcaccatga tgcaactcaa tacttgacta gaaaacggga tcggctgccc
     3481 attcgcattc aatatgatat cgctagaaaa gttattaatg aattgttgga tcgaaaaggt
     3541 gctgatagct actacaataa ataaaaataa tggcactaat ttacaaaatg tgacgatagc
     3601 attgacaaag ctagcatttt caactccacg attcactagc catgcatatc cccataagaa
     3661 gatcgtactc atcacgatcg atgccacatt ttgaccacct tcgaatacgg ggaagaaata
     3721 acctaatgca ctcatcaaca atgttgcgaa cgccacgttt cctaaccagc atgtgagcca
     3781 gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa ccttcttctg cgtaactaaa
     3841 gatcccgcct tttaaatctg gacgtttttt attgatatta ttgatcgata aggagagcat
     3901 caacattgct gttcctacga gtacccagct gaagatagct gggccaccag ccgatccgga
     3961 gatgtccgtc ataataccaa atacgccacc tccaatcgct gaaccaatca ctaaggcagc
     4021 aagtggaatc aaaccaattc cttttttctg ttgctctgta tccatgtaag catcctccaa
     4081 agtaaaaccg cttttatcaa aaagtataac aaaccttttt tatttttact taattaataa
     4141 aaactttaat agaaatataa ttctaccgac ttttttctca ttatacaagc aaagcgccta
     4201 gtccctttcc aaattacacc accaaaaaaa cttaaaaaga tgctatacta aagggaaaga
     4261 attgggggat gatccatgaa cattttatat atatcgattt caggaaatac gcgtgctttt
     4321 gccaaacatc tagcagaata tgctgaaaaa atgcatgcag aagatcctgt caatcctgaa
     4381 gttactttaa aagaaatcca tgaaaatagt gattttacga aagaaacaga accatttttt
     4441 actttcgttc ctacttattt agacggcggg aatgggttag acaacggtga cacagaaatc
     4501 ttgaccgaaa cgatgcgcga atatctggaa tacgaagaca atcataaact ttgccttggt
     4561 gtagtcggta gcggtaataa aaacttcaac aatcaatatt gtctgacagc caaacaatac
     4621 gcacaaagat tcggctttcc tttcttggca gattatgaat tacggggaac accatctgat
     4681 gtgacacgcg tttatcaaat tttggcagaa aaaaataaat aacacaaaag aagaagctga
     4741 aaaataaatt tcggcttctt ctttttatgt tctctattgt ttctattctt attaagcttt
     4801 gacatccatc acaggtgctt ttgctttcgc tttacctgtt ttttccaaga caaagttttt
     4861 tacacgatcc atattggtta ccaagacgat catggaggtt tctttgtctg ctgctttgat
     4921 tgcttctaag tcagcttctg ctacaagcgt atcaggtgtc acttgctgtc cttctgttac
     4981 tttcacatcg aatggcgtac cgcctaagtc aactgtgttg atgcccatgt gaagcaagat
     5041 ttctaagcca tttggtgtcg taatgccgat agcatgtttt gttgggaaaa cagaacttat
     5101 tactccagta accggggaat agatatgtcc gttttctggt tctacaaagt aaccttcacc
     5161 catcattttt tgtgcaaaaa ctgggtcact tgctttatct aaagaaacaa ctgttccgtc
     5221 agcaactgat gataagatag tcacactttc agctgaagct tgaggtactg ctttttcagg
     5281 tacttcattt actgtggctg tttcggtcgc tagttcttct ttttctaaac ggtctttagg
     5341 aataccgaag aagaatgtag caacgaagcc accagcgtaa gcagccaata aaccaaggat
     5401 atagcctaac catttaccat cagagatcaa tggaatcaaa gcggctcctg aaggtccaat
     5461 agcgattgct ccgacatttc cgatcattcc gatcactgcc ccaccgatac ctccaccgat
     5521 acaagcagta ataaatggac gtcctagagg taatgttact ccgtaaatca atggttcgcc
     5581 gattcctaag attccgactg gtaaagcccc tttgatcatt tctgttaatt ctttgtcttt
     5641 tttgcagcgg atccataatg ccaatgctgc acccacttgt cctgctccgg ccatcgcaag
     5701 gattggaagc aataaggtac gaccagtttc atcaatcatt tgtacatgga ttggcgtcaa
     5761 gatctgatgt agtccgaaca tgaccattgg caagaataat gtacctagaa cgaagccggc
     5821 aaatgctcct ccaacaccaa gtacccagtt gatcccaccg accatgccgt cagaaatgaa
     5881 ccctgctaaa ggcatgatga agaaaatttc gatcagaccg atggcaatca gggaaagagt
     5941 tggcgtaacg atgatatcta ccgcatcagg tactacttta tgcagttttt tctcaacgat
     6001 tgacaataac caaactgcga agataacacc taagatcccg ccttgtccag ttgcaagagc
     6061 ttcaccggta aacaagtttt tcagtggtgc ttctggattc atccctgtca gcatcacaac
     6121 agccccgata actccaccaa gcgttggcgt tgcaccaaaa acttgtgcag cattgatacc
     6181 tgtatagata actagatagc taaacatccc gtttttcaag atgttcatca catcgatata
     6241 ttgttgccat gttcctgcgt ctagtgttcc agctgtgact aagtttgcta aaactgcggc
     6301 aatccctgcc acgatcccag tgccgacaaa tgcagggatc attggtacaa agatattaga
     6361 gatgtctttt aataccgatt tgattttaga gggtttttgt ttcgattttt gtgccgcttt
     6421 catttcttgt gcccgttcgt ttacacggtc ttttccagat gcagaagaac ttgctgtagt
     6481 tgcactagca gaaggaagtt tttctcctaa tttcacgcct gctaagtcaa ccatttcctg
     6541 cgctactttg ttcactttcc caggtccgat gatgacttgg agctgttcat cctctactac
     6601 gccaagtact cctggaatcg cttttaattc cgagacattt acaagatcat cattgatgac
     6661 cttcatacgt acacgagtca tgcagtggac aatgctttca acattgccca taccgcctgc
     6721 tccttgataa atttgttttg ccagttcttc caaagtcaat tcgttttttg ccatggtctt
     6781 ttccccctca taaggttcct tttacaaaac cgtttgcttc agctaatttt gcttgagctg
     6841 cctctttatc actatttgtc aaaatcatga cgatagccag tttgacattt tgatctgctt
     6901 cctcaaagta cttttcagct gtttcgtagt cagcttctgt cgcttgcatg atgattcgtt
     6961 ttgagcgttc aattagtttt tcatttgttg gtttgacgtc gaccatcaag ttattgaata
     7021 ctttaccgat accgatcata ctgatagtag aaatcatatt caaaatcagt ttttgtgctg
     7081 ttcctgactt caatcgagta gagcctgtca aaaattctgg tcctgcatcg acttcgattg
     7141 ggaaatccgc atgctcgctg atttctgctt gtttattgca agaaatcgtt cctgtaacag
     7201 caccaatcga ttttgcgtaa tctaatccac caatcacata tggtgtacga ccgctagctg
     7261 cgataccaat aactatatca ttttttgtta aattcagctt gattagatct tcttgcccta
     7321 attcataaga atcttcggct ccttcgacgg ctacagtcat tgcttcttct ccaccagcaa
     7381 ttaggcctat gaccatttcc ggttctacac cgaatgtcgg tacacattca gcagcatcta
     7441 atacaccaag tctaccactc gttccagctc ccatataaat caagcgacca ccttttttaa
     7501 aggattcaat cgtgttggcg atcactggct cgatacttgg cagttctttt tcaacagcag
     7561 cggccacttt ctgatcttct ttgttcatga gttggactgc ttgagaaata ctcatttcat
     7621 ctaatccaaa agtttcttgg tttctgcgtt ccgttgttag gttctctaaa ttgattttgc
     7681 tcatttttat cttccttctt ttcttataaa ttgatagttc ataccagctt tgatttggtt
     7741 gatcagcggt agttcttctt tgatgacttt ggctactaca ttcacttttt catccgctgg
     7801 caaatcatat ttagttaatt ggatctcacc catatagcgc aaatactttt cgttatctaa
     7861 agtaatagat cctttcatac gcttagctgt atttcttact gggattggcg gaatctctcg
     7921 aaaacgcgca tctgcgcttc gtatgacatc tcttgcatca tcttgccgat tcgtatgttt
     7981 tcctaatata tattcgtaaa aaacttcatc tacaggttcc gtatggagca agactgtctg
     8041 ttctttttgg aaagaagcaa attgctcctg aacgtgttct gacagacctg catctccgat
     8101 ataaacaata tccgtgttac ttgctaatag atcaagagca cctgccagag gatggatccc
     8161 tcgatgttct tccaaagttg gtaatccttg atacagtgga cctctcaact ctgtatctcc
     8221 tggtacaaag ccttgtacaa ggaatccttg agttttgagc cattgatttt tttcctgata
     8281 ccattctttg tccaaccctg tttcaagacg tggataataa ttatgccaag cttcaatctg
     8341 tgagaaatca gccgctgctt ctttcaactc atcgatatca ttaggcgtga tcgtgctggc
     8401 attcaaactg attttcaatt gatgtgacca gtcggcgatc tgctgattag aaatatgata
     8461 atccattctt aaccctgtta caccaattgc tttcaagggt gttagatctt ctaatgaaaa
     8521 gccagctcgt ttcaatgcct ctcctgaaat atcgaccatc aactctagtt tattcttttt
     8581 cgcttcgctt cctagttcga tcaatcgatt ttgataagcc gaaacatcat cttccggtat
     8641 atgcatggaa gtgaaaatac cgacaaagcc attggatgcc atttggcgga tatactgttt
     8701 cttctcttct gacaaagaat cattcataaa tattgaaaag ccaaacatat tatccctctt
     8761 tttctgtatc cgtcttcatt tctatgacta gagtatatat ctaaagaaac aaaatttcaa
     8821 tacttttgtt aaaataatta aattttattt ctaaaaattg gatttataat gccaaaagaa
     8881 ttgacacagc cacaaaaaag gcactgctaa gcaaaagcag tgccttgaaa cattttattt
     8941 aaataacaca aaagtctaat tactcataag aaattaattc tgtattgtct accatgaatg
     9001 taaagaaccc ttcattaggc attggatgaa aataagcatt gatcactctt atcaaggcat
     9061 tatgacagac taacaagtag acattttctt cgtcttccat gcattctttc aataaaggaa
     9121 cgatccgtgc gtacacatct aagaccgact ctccatttgg gaaacgtaca gcaaacgcta
     9181 aacgcgcttt ttgaaagttt tcatctttgc ttggcagtcc gtcataatcc ccaaaatcca
     9241 tttctaccaa tcgctcatca gcggtcatcg gcaaattgag acgatcaact actgcttgtg
     9301 ctgtatctct tgctcgttga agaggggaat ggatgatttt cgtgattgga acaggcaaat
     9361 ctactacctt ttctgctagt gcctgtgcct gcatctgtcc tttttcagtc agcggcacat
     9421 ctgctcgccc gcaaactaat ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat
     9481 aaagcatgtg attctctcct tcgttgacaa agttctcctt aaccatacct tgttgtcttt
     9541 gagattacaa ggtttagatt ttctcaagag aaataatctc agtagatact ttatctagaa
     9601 aggtttgatc atgttccact agtaacatag caggtttcac gttcaaaatc agttgttcta
     9661 attgttcttg attgaagaca tccaaataat tcaatggttc atcccatata tagagctcag
     9721 ctggttggga taaagattta gccaattcca cttttttccg ttgtcccata ctcatctgtt
     9781 cgattttatt atgaaaaaca tctctttcca tcccaagctt tcggagatta ttcaaaaatg
     9841 cttgatagtc tactcggttt ttcttcgcga attccgccaa tgtccctcga ttgtcttcat
     9901 aattttggcg tacgtaacta atggtcaaat gtttgggctg ggatttttct cctacaacct
     9961 tgccgttgaa tgtccccaga agataatgga taatggatga ctttcccgcg ccgttaggac
    10021 ctgaaattgc catccgctga tgaggttcga tcgtaaaatg aactggctcg aataacagat
    10081 tttcataccc taattgaaga ttttctacgc ttaacagccg tttatgatga gacacttggc
    10141 tattcatcgt caaagcatcg atatactcga tatcttttaa taatttttct ttctcactga
    10201 tctgggtttc catccgattc acgattgctt tagatcgttt catcgtccgt gcagcatcag
    10261 cgcctactgc tcctttattc actcgcctag attccgtatc gatgaatccg acttttttct
    10321 ttgttttgtc cccttctcgg gaacgagacc attctgcttt ttcagctgct gttttcttta
    10381 gcctgctgac ttcttttttc aatttttcat ttttagctat ttcaaattca tcacgaagtt
    10441 ttttctgttc ttcatagata gagaaattcc cttgataaag ttccagttgg ctcttttcga
    10501 ttgctaaaac atggtccact acttcatcga taaatccccg atcatgactc accacgatga
    10561 atccttgttt tttctttttc aagtaagccg ctacttgttt tctgccagag atatccaaat
    10621 gattcgttgg ttcatcgatt aacgggaaat gagtgtcatc cacaaataag agtgccagta
    10681 ggacttttgt cttctctcct cctgatagtg tgctgaattc tctccataag atttcaggat
    10741 ctgtttgcat cagttggagc tctctttcaa tttcccatat ctcaaaatcc ataatctcat
    10801 ttaacacata ataggtcaaa cgctctttat cttttgtttg ctgcgggaaa tagacaaacg
    10861 cttgctgatg gatcacttgc ccttgataag ggagtttgtt ttgcaggata ttcagtaaag
    10921 ttgtctttcc tcgaccgttt cgtccgataa gtcctagttt ccattgtgtg tcaaagttta
    10981 gattggcttg ttcaaataat aatgtgcctt ggctgtcata gccgaatgtc agatttttta
    11041 tttcgatttt agacattatt ccatcacctc gtaaaaaaag accagtccta ttttcagaac
    11101 tggctaaaga ggatgcaagg gttggggcac acaaaaagac ccatacatcg aaagaaccaa
    11161 gccaatcccg aaaatctgca cactgctccc ttgtctccac aaggtacagt ctgaatgcag
    11221 ctttcatcaa tcgacttaat ttttcaatgc acggatccct cactttgtcc tatatttgaa
    11281 attacgatag cataaagaaa ttgtttttgt caactacttc gggagaatgt tgaaaaatct
    11341 gctgagtgtc tgtcctaaga tttctgtttt ttctaatgtt tgcctgtgtt ccattgcttc
    11401 ctctaaatcg attggtccta attgaatgct taagacaatg gggagaaaat caaagagtcc
    11461 ttccagctct cggtctctac ttccgcaaat agcgattatt ggttttcgat atttttttgc
    11521 aagcactgct acgccatatg gaacttttcc gtggattgtt tgctgatcaa tccgcccttc
    11581 tcctgtaatg atgacatccg cttgttcgat ctgtttttct aatccagtga tttggctgac
    11641 caactcaaaa ccagagacca tcttcgctcc taacaaaaaa agggctccgc caataccgcc
    11701 tgctgctccg cttcctggga tgtcatctaa atccacctcc caaacatctt ttatctgctg
    11761 agcaatcttt tcagcttgtt tatccaagaa agctaacgtc gtatccgttc ctcctttttg
    11821 tcttccaaat tgccaagcag ctccttgttg acctgtatat gggttggtca cgtctgcagc
    11881 gatgatcact tttactttag aaacaagaac gttccttcga tccaaatgaa gttttctagt
    11941 agataacaaa ggatttcctt ctgtgtaccc ttctaaagaa gcacccaaag attggagtaa
    12001 tcctagacca ccatcgctac atccgcttcc tcctaaagtg acaatgattt gttccacttc
    12061 ttgaagcagt gtatctttga taagctctcc caaggcataa ctagaagcaa gtcggatggt
    12121 ttgatccgtt ggtttatcaa gtaaatctaa tcctaatact tgtgcagatt caatgactgc
    12181 ggtatttttt ccttgccagt tcgtcaataa ataactgatt tttcgctgac gaaataagag
    12241 atccatccca tcaatcgtac gccaggttcc cccgagcgcg taatagattg cttctgcagt
    12301 attttctccg ccatctgcaa tcggcacatt gatttttctt cctttacggt cttcccaacc
    12361 atttagaact gcctgattta attcaaatga agaagcactt cctttaaatg aatcgattgc
    12421 cgccaaaata tccatctata tccctcactt tcttcttaag taagacactt tttttcactt
    12481 ttttcaaaca aaacaatgct attttcttct gtttttctat atactaaaag ggaactcatt
    12541 gaataaagga gagaaaaaac atgaaaatcg caatagctgg agccggagca atgggcagcc
    12601 gaatcggatt aatgttgcat caaagcggaa atgaggtcgt cttgatcgat cgctggcctg
    12661 ctcacattga agccattcgt acaaatggat tgattgctga ctttaatggc aaagaagtag
    12721 ttgcaaaact gccaatctat tcaccagaag aaatcatcga aagcaacaaa cacgtggatt
    12781 tgatcgtagc cttgacaaaa gcgaatcaat tagatgatat gtttcgttcg atccaatcca
    12841 ttattactga gaatacttat gttttgtgtt tactgaatgg attaggtcat gaagatgtac
    12901 tggaaaaata tgtaccaaag aaaaatatct tatttgggat cacgatgtgg acagctggtc
    12961 tagctggtcc cggaaaagtc acattattag gtgatggaga aatcgaacta gagaacttgg
    13021 aaccagaagg cgaagcattt acaaaaaaag ttgtagaagt ctttcaagaa gcaaaattga
    13081 atccaatcta tagtcataat gttcgttatt ccatttggcg aaaagcttgt gtaaacggaa
    13141 cattaaatgg attatgcacg atattggatt gtaatattgc tgaattaggt gcgcaaaaag
    13201 cggcagaggg catggttcga acgattgtat ctgagtttgc ttctatcgca gcaaaagaag
    13261 gaatcacatt agatcaagaa gaagtgtacc aacatattgc ttctacttat gatcctgaca
    13321 acatcgggtt gcattaccca tctatgtacc aagacctgat caaaaaccat cgtttgactg
    13381 aaatcgacta catcaatggt gccgtttggc gaaaaggaca aaaatatgat attgctacac
    13441 catattgtgc ctttttgacc caattagtcc atgcaaaaga aggaatcctt ggcgcagaat
    13501 aaaaggctct ctgatgagaa tcagctggat cagcagctgg ttctcatttt tttaccccca
    13561 tgaaagttca aaaagaaaaa cacaattctc atgttgccat aagaattgcg cttgtttatt
    13621 ttcatctatg attggttggc acttttattt atttttaaca gaggaaagat cgtattcata
    13681 ttgttcataa atagcggagt cagtagtgaa aaaacgtttt gcccaatttt ctgcatcaaa
    13741 aaattccaac agttctgatg ggattggttg aaatggcgta tgcaaaaatt ccggtaattc
    13801 atctaaatca cgttcttgaa gcaaaaaaat attttcagaa tgataaaact gataatgagc
    13861 tatggcttga ttatctgtca ctagtttctt ttgaaaatac atagattcaa acgttcgcaa
    13921 agtcactcct tgctgccctt cccgcaacag ttctagaatc cccctcgatt ccccggtttt
    13981 tttcagatat tcttcataag taaggtatcc aggttgttca tttcctcgaa gcggaagaat
    14041 aaagtaattg gtcgtaacac ctctttttgt aaattcctgc ttatattttt ctgcccgttc
    14101 ttttctgccg ttatttgttg caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa
    14161 atccatatgt ttcgaataaa aggaactatt atgttttagt ccaagttctt tcgcttctaa
    14221 aaaatcaaaa tgataaatct catcgattgc cggttcatct tgtgcctgct tcagcatcgc
    14281 ctcgtgttct gctttaaagt ggttccaaaa aaataacacg atttttgcat tggtaaatgg
    14341 acgcacgcgt cgagccatct ccatatcaaa tccataatct aaaaaaacga tcagctgata
    14401 atttgccaac tgttgtcgag ctgatttaat gaattccgac tgtagtaaat cttttccgga
    14461 ccaaacatct aaagagcgga tggaaggaaa aaaataaagt gatttatttt tcgcaattag
    14521 caatacgtcg tttttgttca tctgttccgc ctcactttct ctgtcacttt ttcctatgat
    14581 agcctgttta aatagtaaaa agccagtaaa acgcttccta ttatttttat aaaaatttaa
    14641 catttctatg aaaatacgat gaaataaatc attcttccat taaaatatgc ccaaaagttt
    14701 tctgaatagt tgtaattatc tgaaaatttg ttatcattag tcattattat tattactatt
    14761 tattcttact atttattctt actatgcatt ttgattgtga atttaaaaga aagaggttat
    14821 ttatggaaga taaaaaattg aaaaaacaac ttagctcccg acatatcacc atgctagctt
    14881 taggtggagc gattggtgca ggtctattta aaggaagtgg cgaagcgatt ggaatcgccg
    14941 gcccttctgt attgatcgct tttttgattg gcggcataat tttatatatc gtgatgaaag
    15001 gtctgggaaa aattgtttta agtggtggtg atacgcacca cggattatct ggattgattc
    15061 gtccatatct aggtgctcat gctgccgatt ttaccgattg ggtctactgg tccatgtgga
    15121 ttatcaatat cattgctgaa gctgttgcag ctgcttcctt tttacagttg tggttccccc
    15181 acatcccagc ttgggtattt gtttttatgt tggctgtttt aactactatc atcaatcttt
    15241 attcggtacg attattcgca gagacagaat attggctggc ctttgctaaa atcagtgtca
    15301 ttatcctatt gatcattttt gctgcttatc ttgtcggtca gcaaatgttg ggtactggtg
    15361 ttttccctac ccttcaacaa ttaactgatc atggtggttt tactccccac ggtatgaagg
    15421 gaatcatcag ttctttgctt gtagttatct attcttacgg cggttctgag ttgattgcta
    15481 tcacagtaag cgaagcagat gatcctaaac gtgcgattcc taaagcaatc aaaggcgtca
    15541 tgggaagaat catttccttt tacattattc ctttattttt gcttttgatt atttttcctt
    15601 ggaatacttt agcaggcacg aacgttagtc cttttgtgat ggtatttgaa aaaatgaata
    15661 ttccatttgc ggcagatatc gtcaatttcg tgattgttct tgctctgttc tcgtctatca
    15721 attcaggtgt atatgcttct tcgagaattt tgttcttccg cttgaaagat cggaaaggtt
    15781 cctctagaaa actagctgtt ttaaacaagc atcaagtacc gcaacgtgct gtgctgtttt
    15841 gtaccagtgt tctttatcta ggtgtggctc tttcttattt tgtcggtgac aaattatttg
    15901 gctatctggc aggttctctt tcctatacag tattattgat ctggatcatt atcagtgctg
    15961 cgtcctttgt actgacacta aggaaaggaa cgttatggga caaaggaatc agcttttttg
    16021 cattagcagt cttatggttg attttccttg gtatcctatt cacgaactcc attggggtaa
    16081 ctgtattgac cggtttgctt tacctgttca tttatttcag ttatcaaaaa aagaatgatg
    16141 ctttcgtctt gacgaatgaa tgaaaccaat agagaatttt attctattta tattgaatat
    16201 ctggagctat tgaaaaaaag gagctgcgac aaaaattgtt gcagttcttt tttccgaata
    16261 aacggtgttc aaaaaccatc tgtgcagatt aattgattgt tctccacgct catgacatga
    16321 tagaagaaag gaatgaagaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt
    16381 gttgatgatt ggtgtttcga cggtagtcat gtttggtatg atgtatttga atgtttatgc
    16441 gcttgatcat atcttcttca gtcgcacacg tgtattcatg gcgttgatga tgggtgcagt
    16501 catggcagtc atcatgttgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgc
    16561 tatgatttta gccgctagtc ttcttgtttt tgccggttcc ttatttatgg tacgaagcca
    16621 aacaactgtc aacgatacgg cgtggatgaa ggcgatgatc cctcatcact ctattgctat
    16681 cttgacaagt aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat
    16741 cattcaggca caagaagaag aaatcgacaa aatgaaaaaa atgatcaaag aaatggaatg
    16801 accattatcc atccaaataa aaaaagagga tgttccagaa gtgatcaact tcaagaaata
    16861 agaagaagct acttctggaa caccgtgaat cagaggttgt gaaaaagctg ccctgcatca
    16921 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt taggcttatt
    16981 accgcaaatg caagcttttg aacaccgttt attcgtaaaa gggagtgtga caaaagtctt
    17041 gtcacactcc ctttttacgt ttttattgtt ctaattgcgc tgtcttgctt actgcttctt
    17101 gcggtttcac aaatttcttt gagatccccc aacctgtaaa ggcactgacc atcgaaaaca
    17161 caggtgaaag caaactgaaa aacacgaatg gcaagtaagt gagtgtagga atgccaaatg
    17221 tacttgctac gaaacttccg gcgattcccc aaggcactaa gtagttgatt actgttccac
    17281 catcttctag tgttctccct aatacagttg gatcaaatcc tgctttttcg taagtttctt
    17341 taaatgcatt accaggcagg ataacagata gaaattgttc tccaataaag atatttactc
    17401 cgatgcttgt catcaatgtt gtaaaaatca attttggggt gttcgtcatc tttcttgata
    17461 ctcgttccat aacggttgag atcaacccaa attcgatcaa caaaccacct aatgacaatg
    17521 ttagaataat caatgacaca gttggcatca tgctttcgat tcctccacgg ctaagtagtg
    17581 aatcgatttg ttggtttcca gtatttgaaa caaatcccga ttcgatgacc cctgccaact
    17641 tcgttaagga agtttgtggg ttttgaataa agatcattcc tactgctaca gcaatattca
    17701 acaaaatcgt caaaattgcc ggtacctttt tccatgcaca caagagcatt aaaaatagag
    17761 gaatcagtga ccagatcgaa atagtaaagt ttgcttctaa aatatcagtc acttcacgaa
    17821 tagatgaaag acttgcggct ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa
    17881 caacaaatgc cggaacagtt gaccacatca tatgcttgat gtgtttgaac aaatcggcat
    17941 caacaatagc tgctgctaaa tttgttgatt cggataatgg agacatcttg tctccaaaaa
    18001 cggcacctga aacaatggct ccaacaacaa gggctggatt tagtcctagt gttgtcccaa
    18061 taccgaaaaa tgcaatccca atcgttgaca taacagtaaa ggcactccct acagcgctac
    18121 ctacgattgc acaaacaaca aatacagaag ggacgaacca ctggacacta atcaatttaa
    18181 atccaatgac catgagggtt ggaataattc ctgcttgtat ccatacagca atcaatgaac
    18241 cgactaaaat aaaaataaag attggaatga tccccggttt gatgccattg atgatccctt
    18301 cgtgcagcca atctgtcggg tattttttta tcactgcgta caacatgacc attccgatag
    18361 ctgccaaaat cgtgatatta ggtgaaacct ttaatccgac aacgccaatc gcaatgccta
    18421 aaacaatgac catcaataca agtgttgctt ctaaaaaact tggtgttcct tttttttcca
    18481 tgataatctc tcctttttta gttgttctta tgttctaaaa aagttaagat atcggattac
    18541 tccgccacaa ttcatccgcg attcctcctt tataaaaata aaaagacctg ctttcaacta
    18601 aggacgaatg actccgcggt accaccttaa ttaaaaacag atctgttttt cactttcaaa
    18661 cgattattca aatgtatgga ctttccatgt aattcgtcaa ttaggcttga tcggttcaca
    18721 gcgaccaccg actttctgaa caaaacacct aaccactact gcgtgaaaag ttgacttgta
    18781 tgaagtttac cttccataag atcatttgtc aatgaaaaag aaaatatttt ttcatttttc
    18841 tgaaaattat ttttataact ttcgcgcaca actttatctt tttactagaa agtagcgccc
    18901 tcttgttttt ttcttgctac tatttcttga tgttcttgtt tgattttttc taatagcttg
    18961 ggttctctca ataaatctag agctgtattg gcaagtaatt ccgctgcaat cgcaatagaa
    19021 gcaagtcctt tttcactttt agcagcagct ttgaattctt ctgtatgtcc ggcaatatat
    19081 gtctcagaga ttgctacagt aggctgtatc gttggaataa cttggctaac atttcctaca
    19141 tcagaagaac ctagacttgt tctttgttcc gtttctattt cttcatcagg aacccctgct
    19201 tcttttgcat gttggaagaa taagtcatcg aaagaaggcg ttacgatgac atcatcgact
    19261 gcattttgga acaatccaaa ctcaaaagtc gtacccgtgc ttaatgcagc acctcgtacg
    19321 atattttcca ctttttcata tactttgtct aatgtttgtc gatttcctgc acgtaaatag
    19381 aaacgtccag cagcgtagtc tggtatgaca ttggccgcca cgcctccatc tgtaatgatt
    19441 ccatggatat tgacatcttt tggcagttgc aagcgtagag cgttgattcc gttaaacact
    19501 tggatcaagg cttccaaagc atttactcct ttttcaggtg ctgctgctgc atgagaagcg
    19561 acaccgtaaa atttgatatc gactggatca tttgctaaag attctgtcgt tttcccataa
    19621 cgaaaagctg gatggacaca taatgctgca tcgacatcct caaaaaaacc ttcccgaacg
    19681 aagcttccct tagctgatcc attttcgcct ccttcttccc ccggagtacc atacacacga
    19741 atttctccgc caatctcatc aatgaattgt ttcactatgc tggctgctaa tacggagtaa
    19801 gtaccaaata aattatgtcc gcatgcatga ccaatacctg gtaaagcatc aaattctgcc
    19861 aaaaaggcaa ttgtcggacc tggtttattg gaaatataac gtgcatcaaa gcctgttcgg
    19921 tgcccagcta cttctttttc tacccagaac ccttcttttt ccaattgttt gattaaagct
    19981 gctgaggaat aaaattcata attacttact tcaggatggt cgtggatagc tagtgcaagt
    20041 gcttgatatt ctggtaaacg gtattgaatc agatccttca attgatttgt tgagagtgtg
    20101 gttgtcataa aaatttcctc ctatgctagt cgttttttct tattttctcc cgaatgtttc
    20161 ccacgcaggt acgcttgaac cttttgatgt tttttcgatt actttggctg tttcttctgt
    20221 ttgataggca tctactattt tttgatacgt tttattgtct tcatcttctt ttcttgcaac
    20281 gatgatattg acataaggac gtgcatgctc atcgactggt tctaagaaga ttgcatcaga
    20341 agcgggatta aatccagcat ctacagctac accgctattg atgactgaaa tatctacatc
    20401 tgacaaagca cgtgctgttt gggaagcatc taactcagaa atgtctagat tttttttatt
    20461 ctcggtaata tcagaaacgg taggcgcttg gccttttgcc ttgtcaactt tgatcaatcc
    20521 agcagtttgc aataacagaa gagctctgcc gccattcgtg acatcattag ggattgcaac
    20581 ttttgctcca tctttgatat cagccacatc ttttaacgta ttggaataaa ttcccaacgg
    20641 tgcattaacc gtattcccaa tcgatactaa atcagttccg tgttcttcat tgtaattgtc
    20701 taaaaagaat tgatgttgga aggaattcaa atcgatcgat ccatctgcta aagccgcatt
    20761 tggctgattg taatctgtaa atttcacata ttccaaatgg atgttttcct ttttcaaacg
    20821 gtctttcaca tcgtcccata catctgtatc ttccccaatg atccctagtt ttacggtcgt
    20881 ttcttttttt tcagttgtag ctgcttttcc ttggctgcat cctgccaacc aaataattgc
    20941 tacaaatgta atgactgcaa aacgaataat ttttttcatt ttttctcctc cattttcaat
    21001 gtgtgacttt tttaatcagt tgattactga taaattgact caaaaaaact aagaccagaa
    21061 tcaataatgt agcgacaatc gttacatctg tttggaatcg attatatcct ctcgatatag
    21121 caagattccc taatcctccg gcacctactg ctccagccat ggtagtcagt ccgatcacat
    21181 tgatgatcgt caacgctgag acccgaatga taccaggtaa cccttctaac aaataaacac
    21241 gtaaaatgat gccgattggg ctcgtgccca tcgcttctgc tgcttcgatc accccaggat
    21301 caacttctag cagtgcattc tcgatttgac gggcaaaaaa cgggacaacg ccaaaaatca
    21361 aagggactaa agctgctttg gcaccaatcg tcgttccaac taagaaacga gtagtcaaag
    21421 ctaaaagaga aagcaagata atgaaaggga tggaacgaat gatattgatc aacttatcaa
    21481 gtatttcgaa cagcagcctg ttagctagta atccatttgg acgtgtcact actaacgtca
    21541 cacctaagat gattccgatg atcccaccaa tgaatgccgt ccaaaaagac atatatagtg
    21601 tttgctttgt cgcttcgatg aattcatccg gaatcaaata gacattcggc aaatatgttt
    21661 taaaaaattc agtcatttta gtttcctcct tcgatgactt tgatacggtt atctaattca
    21721 tttttttgta aaatagttac ttttacacca gatttaatca aataatccaa tgctttttgc
    21781 cgctgtttgg tttctccagc taaagtaacg atcaaacttc ctaagggtgt ttcttgtaat
    21841 agctctacat taccgtataa gatattcgct gttacttggt acttgctgta aagatgagcg
    21901 attagaggtt cgttcgtctg gttgccgata taacttaatt ccaccaacca tttatttgca
    21961 atctgatcgg caaaagcagc atgggcactg attgtttcca atgcttggtc aaggtgtgtc
    22021 gctgtccgaa taaaatcttt agtcaattct tcttcaggat gactgaaaat ctggatgctg
    22081 ctaccttttt caatgatttt ccccgcttcc ataaccgcta ccttgttgca gatttctttg
    22141 acaacttgca tctcatgagt gatcaggacg attgttagac ctagctgacg atttagtttt
    22201 tttaacagtg caaggatctg aagggtcgtt ttcggatcaa gtgcactagt tgcttcatcg
    22261 cataacaaca ctttaggttc atttgccaga gctcgtgcga ttgccactcg ttgtttttgc
    22321 ccccccgaca gttgtttagg atacgcagat gctttttctt ccagaccgac aagttccagt
    22381 agttcattca ctttttgacg acgttcctgt tttgattttc cagaatactt tagagaaaaa
    22441 tctacgttgt caaaaatcgt tcgactttcc attagattga aatgctgaaa gatcataccg
    22501 atggcctttc tttcttttcg caattctttt ggagagagct ggcttaaatt cgtctgattg
    22561 acgatgactt ctcctgctgt tggtttctgc aacaaattca tcacgcggac taacgtactc
    22621 tttcctgcac ctgagtaacc gacgatccca taaacatccc ctttttcaat cgttaaatct
    22681 acagaatcta ctgcctgtat ctctttttct ttttgtttga aacatacaga aatatctcgc
    22741 aattcaatca ttaacattcc tccttgtcta taaaaaaagc atcctcaaga aaatgaaaat
    22801 agtcattttc ttgaggacgc ctttttgcgt gttaccacct catgttcaag ctgtgttcac
    22861 acacaactcc tcttcaagta ccatcatact ctagcaagat atcggtcgca gccgtcaccg
    22921 ccttacatct atgctcggga tgaattgctc gatggccatt ttccaatagt cgttccttgc
    22981 ttcttctcag ctaccgaagc tctctgtcaa aggttcttct atcgtactta ccatttcaat
    23041 gcaaataata ttcaaaaaaa aaatcctttt actaacataa actatgtcag taaaaggacg
    23101 acgtatcgtg gtaccacctt ttttcggaag tttccttccc tcaagcccaa catctcaaaa
    23161 agagagattg gcgttcgttt taacggtcga acatttccga atttgcctac ttatcgacaa
    23221 attattctcc aaggccattt tcagcttttc ttacttgact cttcacacca gccagagcct
    23281 ctctgcaaag tttccaagct tactttcctt ctcaacgaaa tcaatatttt gttgttggga
    23341 ataatctaca ggaggaaaac tgaaaagtca agcatgaaat ccaaattatt ttttctatat
    23401 ttatttttct gtttttttat ctaacatttc aataacagtt cgaagatctt ttccttccgc
    23461 aagaaggttc aatttttcct tctcttcgac gctataattt cgaacaaaac tggaatcggg
    23521 aatcaaaaca acgcctagtc ctgcacgtga agcggctgtc gctcctacta aagaatcttc
    23581 cactgcaaaa gctgaaagag cttctaatcc tgttcgttcc aaagctgtca gataagggac
    23641 tggtgaaggc ttgttctccg atacttcatc tccgaacgta aacgtatcaa agtaatcggc
    23701 taattgaaag tgatccaaga tatctgttgc tcttttcgta acagttgtcg tagcaatccc
    23761 tagtttcata tgacgttctt ttaagtctgt caatatttct tttgcatagg gtttcaattg
    23821 gatagctcca cttgtcagct gttgataaaa atattcttca cgctttgctc ggacttcctg
    23881 cactttttca ggactgcctg ctgcctgcac taaaatatca aaactttgtt tccatttttt
    23941 tccgccccaa gcgtcaacga tttctttcgg gatatgaata tcaaattgct ggaatcccca
    24001 aagccaacca tcccgatacg ttctttccgt atccactaac aaaccatcta aatcaaatat
    24061 tgcacctttc atctatacat taatcctttc caaaacaata cacctagttt agcatgactg
    24121 atccaaaatg aacagaagca tagagcccag ccgcatcact tataagagtt cttggaatcg
    24181 acggatatag attttcttca cagctgtcac aagcgccaga taagcgacga ctgttataaa
    24241 aaaccataac cagtaattgc cagggagcgg catcaagcct aactcagcac caaatcctgt
    24301 gaatggaagg actgaaccga caatgattcc taatgtagtg ataaccagca tggcaaatgt
    24361 tgcattgctt tggataaatg gtattttcgg tgtacgcaat gcatggagta ctaatgtttg
    24421 tgtccacagg gattcgacaa accatcctgc atggaataat gcgataaaaa tcacttgttg
    24481 tgagctatcc aatgtatgga aacttccacc aactacagca ggacaaataa caaaatacat
    24541 caacaagtaa gtagtgatat caaaaatcga acttgttgga ccaaaataaa ccatgaattt
    24601 tccaatacta gatgcattcc atttctttgg ctcatccaaa tattctttat ccatccgatc
    24661 ccatggtaag gaaatgcaag aaacgtcata gatcagattc aaaaatagaa gttgcaaagg
    24721 caacattggt aaaaatggta agaacgtact ggcaatcagt acggaaaaca tattgccaaa
    24781 attagaactt gctgttgcct tgatatattt catgatgttt ccaaaagttt ctcttccaga
    24841 aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt ctgctgattc
    24901 ttttgcgata tccacagctg tatccacgga aataccgata tcagccactt tcattgctgg
    24961 agcatcattg ataccatctc ctaaaaatcc gacagtatgc ccatttttac gtaaagctgt
    25021 cgtcaatcgt gctttttgct gaggattcaa ttttacgaat acttcgtgtt cttcagctac
    25081 ttgcgctaat tcatgatcat tcatttccga gattttctca ccagtaatca attcattgat
    25141 ttctaggcct acttgacggc acacagaccg tgtgactaat tcattgtccc cagtcagaac
    25201 ttttacagct acaccatgtt cttttaaagc tttcaacgct tgttttgttg tttcttttgg
    25261 tggatcaagg aaagcaagat aaccaatcag aaccatgtct gactcatctt tgactgaaaa
    25321 ttctccaaca acgcttggat tggtcttttg tgcgacacca atcactcgta agccgtcttc
    25381 attaagtgat tgtacttttg caagcaccgc ttcttttcgt tcttttgtta atggtgcgac
    25441 agtaccacca agatcaataa atttagaaat gttcaacatt tcttcaatcg cgccttttgt
    25501 gatcatctgt gttttgccag ccggatcagc gactacaaca ctcatacggc gacgttcaaa
    25561 atcaaaagga atttcatcta ctttttgata attgattgta tctgtatcta aagtttctgc
    25621 tgcttcatca atgatagcca catctaacaa atttttcagc cctgtttgat agtagctgtt
    25681 tagataagca tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca tatgatattc
    25741 aagaatgatt ttgtcttgtg tcaatgttcc cgttttatcc gtacataaca catcgatcgc
    25801 accaaaattc tggatagcat ttaaattttt gatgactgtt ccttttttgg ccatcacact
    25861 tgctcctttg accaaattcg tcgtaacgat cattggaagc atttcaggcg ttaaaccaac
    25921 tgccacagat aatccaaaaa gaaaagcttc taaccaatct ccttttgtaa gcccattgat
    25981 aacgataact gctggagcca tcaatgacat aaatttgatc agcagcatgg atgttttgtg
    26041 gatgcctaat tcaaaacttg attggatggt tttttcagat agcgtttctg ctacatgccc
    26101 aaatagtgtg tcatttccga cagaaacaat gattccttcc gcgcttccac tgatgacatt
    26161 gcttcccata aagacaagat tttcataact tgtttcactt tgctgatcgt cttcacatac
    26221 tttcgcatgc ttttctactg gatagctttc ccctgtcaat gctgcttgag aaatgaatag
    26281 atcctttgcg gacaataaac gcatatccgc tggtatcata tctcctgctg aaagccgaac
    26341 aagatctcca cagacgattt cttccatagg aatctctgta tactcgccat ttcgtttgac
    26401 agctgccgtt actttcacaa gtgatttcag tttttcagct gcttgattag aacgcacaga
    26461 ctggatcaac gtcatcgtcc cactgacgat aaccatagtg aagataatgg ctacaccaaa
    26521 gagatcttta tctcctggag cggctaatac gtattccgta atgaatgaaa taattcctaa
    26581 tgcaatcaac actaatgtaa agggtgtgat gtatgctttc aatatttcta taatgaatgg
    26641 tgtttttttg ccatatgaaa tcgcattttc accataagtt tctcttgaac gctcgatttg
    26701 ttcttctgac aatccatttg gtgatgcatg gaacaattca aataattctt gcttcgtttt
    26761 tccagcaaaa gctgcataga ttttttgtgt atctttcttt ttttccatct cgatctctcc
    26821 tttgtgtcca aaagacagct gttctcgctg tttttggtct tttgctattc atcggatcga
    26881 tagagaaaat gctgaaaggg gctgagaaca gatagataat agccctggcc tttcctctaa
    26941 atccttgttt ttcgaatgac tgtcatccat ttgttctcat ctcctttact aagtaatttt
    27001 aattcatgac ttccaatcat ttttcgaata gttattttcc caactcgctc acctcccttt
    27061 ttgttttttc acctatggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga
    27121 aaggtttgaa aaagcaaatc aaaaaaagct tctcagatct catcgttgag ttttgacact
    27181 gtacaacgta aaaagcagat atctgctttt agctacctta tgaaaagcct tatctcgaca
    27241 attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata
    27301 ggagcctcac ctaacaaatc ctattcggtt ttccctcgtt aatataacaa ctaaaaaaga
    27361 ggatgtcaaa tcaattttcc atgtttttca tactttttta aaatacacgc gttatatttc
    27421 ctgtctcacg cagttttatt tgtctcattt tccacctccc gctataatag aattatagta
    27481 ggaaacgagg tgctgttatg gaacgaacac ttgttattat caagccagac ggcgtaagac
    27541 gccatttggt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa
    27601 tgaaatttgc aacgatgaca cctgaactag caaaagaaca ttatcagcac ttgactgaac
    27661 gtagtttttt tgatgagctt atcgattaca tgacttctgg tccggtcgtt tatcttgtgc
    27721 tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt cggagcaaca aaaggggcgg
    27781 atgccgttcc aggtacgatc cgaggagatt atgcactgcc ggggactgaa aacatcatcc
    27841 atgcttctga ttcccgtgat gctgcaacaa aagagatcgc ccgctttttc ccgccattcg
    27901 atacaaacaa accgtctgaa aaagcggcga agtgatacaa atcctaaact gttggtctaa
    27961 ctgctgggtt tagcaatggc cagcagtttt ttctctaaag aattcctttg attcttccct
    28021 aatgtatatt cgatcactgc gaaaaactct tggtgatttt ccgatttttc ttcccctaaa
    28081 gcagcagaaa tcagtcgttg gtaataatcc gccatcaatg tttcataaat cactgtactt
    28141 ctttctttat gcgctaaact ccgtttcata tcttcattca cgctacgtgc cttttttaag
    28201 cgaccatttt ctatataaaa caaagtcaag ttcgtcaaaa aagtaaagag atactgctcg
    28261 tgattattca gttgcgcgat ccgtcgcatt ctttttttga ttttcaaata attattctca
    28321 ataaaacgat cactgaatat ccaaggaaaa tctgctagaa tagatacttc aaattgccca
    28381 tactctggta cttgcatcaa atattcttca atgatacgac attcataacg caaaaattca
    28441 aattccgtct cagatacttc tgtttttttg gacatgtgca gattcaaaac caaatagtac
    28501 agatcttctg ttttcttata gtgcctgcgg ttattagcaa tttctttgta aggatcaaag
    28561 ctgtcttctt gaatccgatc tagcattcgt ctttttttct ttaatttttt tggctcatca
    28621 agctgatttc gtatccattc atattcggca agtgtcatgt tcattcgctc taaaaatgat
    28681 ttcaatgttc caaacgatac ttttttcact gaatttgtct cgatcccgac tagcgaagtt
    28741 ttagaacaga ttccttccgc taattgggtc tgtgtcagct ttttgttgac tcgaagcttc
    28801 ttaatcaatt ctccatcata cataatagtt ctcctttttt aattgaaatt aacccttggc
    28861 ttttccagaa tgcttgccaa taagaggaat gattatttca atttaaattt tccgcattca
    28921 ataaatagat acgttaaaaa taaaaaatac acgaaaaaaa taaaaaaatg tttttctcat
    28981 aaaaaatacc aaccttcaag catgagactg ctgtctaatc cctgaaggtt ggtttgattg
    29041 tttattgatc atttttttac tgctccgcat tggttcctgt tactggatat aggatagtcg
    29101 ttgttttatt gccgtttttt gaaaagacac tggtcgaatg ttcacctttt tgcttttcga
    29161 ttgcttcatt acgttccgct tcgtttttat agtcatattt tttcggatca atcgtctcaa
    29221 atccgtttgg cacataaaac cgcaagagat tttcctgatt cagcttatct gaaagactta
    29281 acgcagtctt gactttttcc tgatctttgg agatttcctc gtcgattcct gctgctttcg
    29341 tatcgatgat ttctcctgtc tgattattgt aagcttttcc tcctaggacg gtgtatttgg
    29401 gcgtcacgaa gttcccattt cgaaaagcaa ctacttgatc gtgctgagaa gataataagt
    29461 ctgtaccaaa atgaagataa tctttatcat cgattcccaa tagatgtagt aatgtcggca
    29521 aaacatcgat ttctccacca tattcatgat tgactgtccc tttagtgtaa ccaggcatat
    29581 ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat gtatctggat
    29641 ctttaccaag agcacttgct aaatctttat tatctgtatt cgagatccca aaatgatccc
    29701 cgtaaatcac aaagattgaa ttttggtaaa taccagattt tttcaaatac gtaaaaaatt
    29761 gttccaatga ttgatccaga taatgggccg ttcgaacata attatctact gtgctattgc
    29821 cagtattcaa agatggaaag tcgaaattct tgtcatccgt ataataaggc gtgtggttgg
    29881 ttacggaaag aaatttcgtg taaaacggct gttgtagatg ttcaagatat tgggcagatt
    29941 ctctgaataa atctttatct aatatcccat aacctaacgt ttcatcggat tcatcaaaat
    30001 aagatcgatc aaagaaattg tcatacccta aatttttata tacatgatct ctattccaga
    30061 aactagctac attcccatgg aaaacagcac ttgtatagcc ttctgtttga ccaagggtcg
    30121 ctggtgctgc ttgaaagaca ttatctgacc caagttctgt gaataatgag ccttgaggta
    30181 aaccaaatgt gccagtttcc aacatgtttt cagcatcact tgtctttccc tgtcctactt
    30241 catgaaagaa attgtcaaaa ctgatcgttg cttgattctg gaaaatacta tttaaaaacg
    30301 gcgtgacttc ttgtccgtct accttcatat tgatcaaaaa ctgttgaaaa ctttctaaat
    30361 gaaggacaat cacatttttt cctttagcaa tcccaaatgt ttcaggattt ggctctgcat
    30421 aatgtttctt tgtatagtct aaaacttcat caattccatt actgcttgca tgcgcgcgaa
    30481 cactgctagt cattcctgtt ttgattccgt cataaatagt gtacgcatcg attcctagat
    30541 atttgacaat ataggaacgg tcaaatgtcc gctgtagcaa ttggggacgg ttggcttcgc
    30601 tcaacgctaa atttcctgaa aaaatcaatc ctgaaagaca ggtaacagcg atagccatcg
    30661 gtttgctgac agggttgctt ttgataggta ttttcttgac cttcaaccaa accagtagcc
    30721 caatgaacac agcgatatcc aaccaataga tgacatcatg gggtttcatc aaagaaaagg
    30781 agcttcctga caacccttgt gacactttcg aaaagcctaa tacagatttg actgtaatga
    30841 aatctgtgaa ttcccgataa aaaataatat tcaagtacaa caaaagcgta tttaaaatat
    30901 caatgatcaa aagaatggct aacgctggct ttggcttttt aatatacaaa gaaaggccaa
    30961 aaaacaataa ggtagtcgca atcgggttga tccacaagat aaagtactgg atcgtacctt
    31021 ccacacctaa tgaaaaatct gcatagtagg caatgatcgt cttcagccag aaaaagaaaa
    31081 cagctgtcaa aacgattgct aaacgatttt ggcacaattt tttaagttgt gtcacgtata
    31141 tcacttctct ctattcacat tttttcttat tttgctcctg aaaaatgaaa atttcctaaa
    31201 aaagttgtta tcttaagatt tttttattct ttctaaagat tattccattc atttttcaca
    31261 gcataaaatc gtttcggtgt aagcggttaa aatagtaagg agataaaaat cttgtggtga
    31321 ccgatttact ctatagtatt caaaagtagt ttctcggtat tcgtacaaaa aaaacatact
    31381 aagattagag gggaacatca ttgacgaatg atgttctcct ctattttcta tttttagatt
    31441 atagtgaaga gtgaaagaaa gttcgaacaa agccttgagg actacgagtc cctatcaaaa
    31501 accgaacttc tttactgtca ttttagaatc aggtttacgt atgttggggc catgagcccg
    31561 tgtataggaa attggaatcg aaatgaagca gtaaagttct ttcaacactt aaaaaggagg
    31621 aaaacaaatg ccttacattc ttgcattaga tgtttcaatg ggacacagct acagtgtcct
    31681 atacaaagac gacacctgct tatttgaaga cgagatcgaa cacactcaaa gaggatttca
    31741 tgcattactc gaagagatcc agcacttgcc tgaacgtcca ttgattgttt ttgaagcaac
    31801 gggtatttat tccagaccag tcgaaaagtt ctttcaagat catcactttt cttactgttt
    31861 gttaaacccg ttagaagcga aaaaacagat ggaagaaaac accttacgca gttggaaaac
    31921 agataaatcg gatgcccatc ggctcgcaca gactcatctt aaaaatcaac gtcaaccaaa
    31981 agaagtacaa agcaattttt acttagagat gcgcgattta gcccgttttt atcaagaaat
    32041 tgagaagaaa atcactcgac tgcgcatgga tttacacaac tgccttcagt tgacttttcc
    32101 agagcttgaa cagttctttt caaacaggct gaccccttac gcattgacat tgatccgtct
    32161 ttttccccat ccagattttg tattggcatc tactcggaca aaaataaaaa ataaactgat
    32221 caacgaaaca cgggaaaaaa tctccgcgaa tcgtgccgag caaaaggcag atcaaatcat
    32281 ccactatgca cagtgtgcct atccagctgt tgaaaaagac agtattcact gtcaaaaaac
    32341 gatctattac gccgaacttc tccaagattt actcgaacaa aaaaaagcac tggcgacaca
    32401 aatgatcaaa aaagccgaag cctctccttg ctttttgttg taccaaacgt tccctgggat
    32461 tggcgcactc acagccgctt tattgctcgg tgaattagga gatatcacac gatttaaaac
    32521 ccataaacaa ttgaatgcct ttgtcggaat cgacatccga cggtatcatt caggtaagta
    32581 tactggccaa gaccgcatca ataagcgtgg taatcccaaa gcacggaaaa ttattttctt
    32641 caccattcga aatatgattc gccagcaacg cgcagctcct aatcatattg tcgattatta
    32701 ctacaaatta aaaaagcaac ctatccctaa gaaaga
//
LOCUS       DAYYGY010000022        32715 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000022 DAYYGY010000000
VERSION     DAYYGY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.022"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            456..1181
                     /locus_tag="ACHA52_001068"
     CDS             456..1181
                     /locus_tag="ACHA52_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM8939810.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            1171..1845
                     /locus_tag="ACHA52_001069"
     CDS             1171..1845
                     /locus_tag="ACHA52_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM8939811.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(2133..2417)
                     /locus_tag="ACHA52_001070"
     CDS             complement(2133..2417)
                     /locus_tag="ACHA52_001070"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM8939812.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2711..4018)
                     /locus_tag="ACHA52_001071"
     CDS             complement(2711..4018)
                     /locus_tag="ACHA52_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8939813.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4507..5613
                     /locus_tag="ACHA52_001072"
     CDS             4507..5613
                     /locus_tag="ACHA52_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939814.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            6204..6404
                     /locus_tag="ACHA52_001073"
     CDS             6204..6404
                     /locus_tag="ACHA52_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8939815.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            6551..9340
                     /locus_tag="ACHA52_001074"
     CDS             6551..9340
                     /locus_tag="ACHA52_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8939816.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            9387..9914
                     /locus_tag="ACHA52_001075"
     CDS             9387..9914
                     /locus_tag="ACHA52_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8939817.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            9935..11125
                     /locus_tag="ACHA52_001076"
     CDS             9935..11125
                     /locus_tag="ACHA52_001076"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8939818.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            11165..12463
                     /gene="asnS"
                     /locus_tag="ACHA52_001077"
     CDS             11165..12463
                     /gene="asnS"
                     /locus_tag="ACHA52_001077"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8939819.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            13397..14071
                     /locus_tag="ACHA52_001078"
     CDS             13397..14071
                     /locus_tag="ACHA52_001078"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8939820.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            14068..14409
                     /locus_tag="ACHA52_001079"
     CDS             14068..14409
                     /locus_tag="ACHA52_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939821.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            14439..14798
                     /locus_tag="ACHA52_001080"
     CDS             14439..14798
                     /locus_tag="ACHA52_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8939822.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            15058..15180
                     /locus_tag="ACHA52_001081"
     CDS             15058..15180
                     /locus_tag="ACHA52_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939823.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            15322..17403
                     /locus_tag="ACHA52_001082"
     CDS             15322..17403
                     /locus_tag="ACHA52_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8939824.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            17714..19180
                     /locus_tag="ACHA52_001083"
     CDS             17714..19180
                     /locus_tag="ACHA52_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8939825.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            19465..19963
                     /locus_tag="ACHA52_001084"
                     /pseudo
     CDS             19465..19963
                     /locus_tag="ACHA52_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            20037..20477
                     /locus_tag="ACHA52_001085"
     CDS             20037..20477
                     /locus_tag="ACHA52_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8939826.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            20490..20699
                     /gene="copZ"
                     /locus_tag="ACHA52_001086"
     CDS             20490..20699
                     /gene="copZ"
                     /locus_tag="ACHA52_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8939827.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            20699..22885
                     /locus_tag="ACHA52_001087"
     CDS             20699..22885
                     /locus_tag="ACHA52_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8939828.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            22905..25076
                     /gene="copB"
                     /locus_tag="ACHA52_001088"
     CDS             22905..25076
                     /gene="copB"
                     /locus_tag="ACHA52_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8939829.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(25188..27359)
                     /locus_tag="ACHA52_001089"
                     /pseudo
     CDS             complement(25188..27359)
                     /locus_tag="ACHA52_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(27349..28101)
                     /locus_tag="ACHA52_001090"
     CDS             complement(27349..28101)
                     /locus_tag="ACHA52_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8939830.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(28098..28421)
                     /locus_tag="ACHA52_001091"
     CDS             complement(28098..28421)
                     /locus_tag="ACHA52_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939831.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            complement(28435..29310)
                     /locus_tag="ACHA52_001092"
     CDS             complement(28435..29310)
                     /locus_tag="ACHA52_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8939832.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            29637..30926
                     /locus_tag="ACHA52_001093"
     CDS             29637..30926
                     /locus_tag="ACHA52_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939833.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            31000..31569
                     /locus_tag="ACHA52_001094"
     CDS             31000..31569
                     /locus_tag="ACHA52_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8939834.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(31745..31921)
                     /locus_tag="ACHA52_001095"
                     /pseudo
     CDS             complement(31745..31921)
                     /locus_tag="ACHA52_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            32000..32698
                     /locus_tag="ACHA52_001096"
                     /pseudo
     CDS             32000..32698
                     /locus_tag="ACHA52_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT        10845 a         5712 c         6798 g         9360 t
ORIGIN      
        1 atatagaaaa gttagttact aaatagtcaa tcagtttttt cacgacgttt ctattataac
       61 gaattatgtt gaaaaaagct tttatcgaaa acataaatct tcaaattaac agatttttta
      121 gacctcgatc atgatgaaga cttttgtaga tacaaataac tgtaacattg aaaatgaatg
      181 cgaaaacgat attttttttt attggaaaat atcggtcaag caataaacca gttgcataat
      241 aaaatgttaa cggcaacaaa atctgtggaa ggaatgaacc ggatgatttg atggaaaaaa
      301 caagatagac aagaagtata catagtaaaa cgtggaacaa gtaggccaga atgaaaaatc
      361 agtttttatc cactcgcttt tctatacatt cactgtcaca aatataatcg aaagtaattc
      421 tattttgaga taaagtattt tataggagat agtatatgaa caaaaaaaga tttaaacgta
      481 ttgaaagaaa atttgcatta acttatgacc aagctaagta tttgaaaaca ctatttccaa
      541 aacaggtagt tcgggataaa tatagtgatc attttatttc ttccttgtat tttgatacaa
      601 acgatcatca ctttttaaaa gaaatcacta cagaaaagtc ctatcgagaa aaatttagaa
      661 tcagaaaata tggagatcct caggacatat cgcctatcta cttagagata aaaagaaagt
      721 ttcaaggaat gacagataaa cgtagaataa aattagcgca tcatgagctc gatgatatgt
      781 tggaaaatat cgaattggca gaaaccacat tcagtaattt agaagaagag tatattttta
      841 atgaaataca gtggttgttc agaaggttgg cattggagcc caaagtatat atctattatt
      901 taagagaagc atttgtaaat agagattcag aagatttgcg aatcactttt gatcaaaata
      961 tccattaccg tctaatgaac ttaacgacct gggaacactg tacagaagac aatctggtag
     1021 cgccagaaat cgatgtgctg atggaagtaa aaacgacagg agaaatacct gaatggttac
     1081 aggtaggatt ggataggatg ggtaaaaaag agataaaatt ttctaaatat aaacaagtct
     1141 ataaaagata tatccaagag gacccgctct atgttcaata gtgtcttaaa aaatgagcct
     1201 tctcaactca cactagatga tttcttttta tgtatggtgg tgtcgatttt gctaggcatt
     1261 ctgattgctt acacccatat gtatcaaaac aactgtacaa aaaacttcat gtttgccttg
     1321 attgggttac ctactttagt acataccgtc attttgttga tcaacggtaa cttaggcacg
     1381 gggatcgctg ttctaggcgc atttaactta gtgcgatttc ggtctgctca agggaattca
     1441 agagatattt tcagtatttt ttgggcaacg tcgattggtt tagccacagg gacgggatat
     1501 attgtctatg cactattcgt gtctagtata gttgctgcta tccaagtcat catcttcacg
     1561 cttccaatca aaaataaaca tacagttttt aatactgaaa aaacgatcaa gctaaaagtt
     1621 tctaaatcct tgtttctttc gagtattatt gaagaaaaaa ttgcagaata tacagaaaac
     1681 tgcaatttaa aaaaagtaaa aatcaataaa gaaacttgtt ccttacacta tatagtaaaa
     1741 gtaaaaacga gtatgcttga aaaagaattg gttcgaacga tcatggatca tccagaagtc
     1801 atttcgatca ctgtaggtat ggataaatcg aataaattat tgtgataacc aagaaagtac
     1861 ttaacaatga agtaagaccc cagttccaaa gtttagcggg gacacttcaa taaagtactt
     1921 tcttttttgt cttcagcaac tactggtaaa actgagagca agctttcgtg agagtacagc
     1981 gataaagatc aacctccctt tttatctcga gcaatttcct tgcaaggctg tttcgttgat
     2041 cttaaacgag ttagcaacag tcaaagatgc gatggccata aaaaaattgc attaagttgg
     2101 ctggtatctg atagctggga atctaaggaa gactatccat tctgtttctt aaattccgct
     2161 agttgataga tatcagccaa ttgttccaga tgatcaacat caaagataac cgaaagatgt
     2221 ttccttttat tcccatttgc ttgaagagtt tttatcttct ctttggttcc ttcactcaat
     2281 gagagtgtga ctaatttctc cctcgttttt tctgggaaat gaaaggatac tttgaaaaat
     2341 ccgtcccaaa tagaaagcca gaagatcggt tttactttct gagagtggga caaaagtaag
     2401 aaacactttt gtctcattct ttaaaaacga ataaacggtg ggagcagaag caactccttc
     2461 ggaaataagc caaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
     2521 ctcggagtta aacacttctg tcccaacctc ttgtattcac cttttgatag ccaagctttt
     2581 ccatcattat aaaaccgcaa atttcaaaaa aaaacgaaca atacaaaaag cgtattgctc
     2641 gtttttctct tcagtttctc acacctaaac gacttttgtc tcgacctctt tttggtgact
     2701 attcatgatg ttaaaataaa ttactgaaaa aatctgtgac atggcgccaa ccaatgacaa
     2761 aaatattagc tttttcaaca gtagaagtgg taatgatgtc tgtcgaaggc tcttggtttg
     2821 cttccaaata gcctaatttg tcatcagcta attggatcgt tgctgagcca actgtagtgc
     2881 ctttgttgat aggagcggtc acttggtcat ccgttacttt ctttttatca attgttggag
     2941 taatcgttaa ttttccagta tccattccat tttcgaccca gacttttaca gggcttttta
     3001 gcgcaacatt gacggaggtt tcttttccat ctttcacttt gatgtctttt aggcttggga
     3061 tgcttgcatt tgcttttcca agttcttttt ctgaccagtt gtcataacaa taatccatca
     3121 atcgtgatgt ttcattgaat cgggcacttg gattttcttc atggttatct gcatttaaaa
     3181 cgactgtgat gattcgtcgg ccatctttgt tgatcgttcc gacaaagcat gcacccgcaa
     3241 aatcagttgt gccagttttt aatccatcga caccttcgtg atagtttaca aaaccaggta
     3301 gcatccagtt ccaattgacc atttcaaccg gtgactgtgt gttttcgccg aatgtttgat
     3361 ttgtggtact cgtgattttt aagacttcag gataatctaa gatgaggtga cgagcaacaa
     3421 tagcgacatc tttcgcagac agttgatttt catctgtttc accagtattc tcaggtcgat
     3481 tctcacctag gtaagaattg tttaaacctg atgcattgac gatcgttgca tctttgatac
     3541 cccaatcttt tagttgtttc ttcattagct caagaaattt actttcactg cctgcaactt
     3601 tttcagctaa agcaaccatg gaagcattgg ctgattggat gaaagctgag tcaacaagtt
     3661 ctttcactgt ataatcattt tctttatgca aaggaacatt cgatagatcc ggcacgacac
     3721 ttaatttttc tgcataatcc gaaatggcta ccttatcttc ccaagataat ttaccctctt
     3781 tgatttgttc aagtacgata tatagaccaa tgatctttgt gatggaagca attcccatcg
     3841 ttttttcgcc atcctgatca taaaggattt ttccactttc tgcatctacg gcaaaggcag
     3901 ctttggcatt tattttaaat gcgtcgtctg cttgaacagt tgcgctaggc aagaaagaag
     3961 tgatacataa gataagactt gctaatagta caataaaacg ggtatttttt ttgaacatct
     4021 ttgttctcct catttttaaa ttgattctta caacttagaa tattatcaga gagaacaaaa
     4081 ggtttcaatg gttctgaaaa aaaccttata aagaacaagt cttattaatg aagaatcata
     4141 gaaagttatc ttttgggttc attgacacga actttgactg gctgaacttc gggttcggtt
     4201 aggcgtgatg ctgcaaaaag ggcgcctgct gagagtgcga ccaaactaaa aatcatgaat
     4261 aattgcataa gatcacctcg ttcttttttg tttttttata gtatagcaag agagtttgaa
     4321 gctactgtta aggaaatgag catataattg atgaaagtat gaaaaaaata tgaagtagga
     4381 caacacaact aaaaatcgat caggtatttc tctggtaggg attcgtttta ttgtattgat
     4441 aaaatgggat gtggtagact ttatttacag atacaaagaa atgatttttt taggaggggt
     4501 acgtttatgg caaaaaaaga aaaagaatta ttatcacaat tgacttcaga aggtaccaaa
     4561 ggaccgtttg tcacgattat gttaaacacc catgttgcgc atcaagacgt agaaaaagat
     4621 caaatcaaat tcaagaattt tgcaaaggaa gcgaaaaagc gctttgaaaa gaaatactct
     4681 gagcatgatt ggtctccatt ccaagaaaaa attactagtt tgttggacga tccgtctttc
     4741 tggcgtagtg ccacaacgag tgtagctgtc atagtatcac cagaacaagt gtttatccac
     4801 cgtttgggta ttcctgtaga tgaccagtat tatgtgagtg atctacctta tttattagcg
     4861 atcatcaaaa atggacattt caactattca tactatttat taggattgaa tcgtgattcg
     4921 atgaaattgt acgaagtaac gaataaacaa gtcaaagaag tggctttgcc tgaagatgca
     4981 ccaagagatg tcgtcactgc tttaggatct gaactaacag gaggcgggtt gaactacgca
     5041 tcaaatggta gtgaaggtac gacgttccat ggagtcaatc ctaaagatga agaagtcgaa
     5101 attgattggg tcaattatta tcaagcagtc gatgactatt tgaaagatac atttgaaaat
     5161 ccagagaaat ggcctatctt cttatttgca ttaccagaaa atcagacgat gttcaaaaaa
     5221 gtggcgaaga acccttatta tcgttccgat gttgcgatct cagcttcacc tgcacaactt
     5281 tcattaaaag aaattgaagc aggcgcaaaa aaactatcgc aagaattagc tgtcaaagaa
     5341 gcaagtgact acaataagtt gcttgataaa aagttcattg atcaactcgt tgatattata
     5401 ccggcagcaa aagaagggaa aatatcccat ttattcattg ctacatctaa tttagcagat
     5461 ggttttggcg aagatccaga aacagaatat gaccgccgtc aggtgctgaa tgaactagca
     5521 gataatgtga tcacgaacgg tggtcaggtc tttatccttg aacaaaaaga tgcaccagac
     5581 gaaaagagtt tagttgcgat cttacgttat tgagtgaaaa ctaccaaagt agatgaatgg
     5641 tcaggcgttg atagaagtgt atagctccga gagaaggagt ttcctctgtt tcagccgttg
     5701 gattcatctt gagcgagaga caaaagctag cttttgtctc tcgctcatta tttttgtgct
     5761 ttagtcccat tggctttgcg aggggaagat acttttctca aaaaaacgaa ccttgcagtt
     5821 gagaggggga tagatgcgca tactaatttt tatcagttaa aaaatgatgt ggatcattag
     5881 agaattccaa agttcgttca tcctttttca gataataaat tgaaattttt tgcaaaagtg
     5941 aaaatagcaa atagaaattt tctgaatctt ataggggtga tttcgagaaa acgttcgttt
     6001 atttgcaaat atatcttcta aataagagaa taaatacaaa aaaataagga aaaactctgg
     6061 caattttgta aacggtatga tatagtaaga atgtagtttt tgttcggtaa tctgtggtag
     6121 cttgtttcaa ataaacatct tccagatata caccattcaa gtaattgcaa aatatgtgtt
     6181 gatacacaag gaggattttt attatggaaa caggtacagt aaaatggttt aactcagaaa
     6241 aaggatttgg attcatcact gcagaaaacg gaaacgatgt attcgttcat ttctcagcta
     6301 tccaaggcga cggattcaaa actttagaag aaggccaagc agtaactttc gacgttgaag
     6361 aaggtcaacg cggacctcaa gcaacaaacg ttactaaagc ttaatttacc tatattaaaa
     6421 ataaaagtcc agacatttat gtgtctgggc ttttttgtct ttttttcctt aaaaagcgat
     6481 gagagtattt ttctgcactt tgtagtataa tttcaaaaga acattagagg caggagaaga
     6541 ggaagatatt ttgaaaccaa gaacttatgc agtcgttgat atcgagacga ctgggacgaa
     6601 tccaaaagaa gatcggatca tccaatttgg ctgcgtcatg attgaagctg gtcggatcac
     6661 ttcacgtttt tcgatcgata ttcatccagg gcggaagatt tcaaaacaaa tccagcattt
     6721 aacagggatc acgaatcaac gcgtccaaaa agcgccttat tttgaagatg tcgcccaaac
     6781 gatttacaat cttttggcag acaccatatt tgtcgcgcat aatatttatt ttgattataa
     6841 ttttttgaac catgaactca tgcgatgcgg acttcctagt ttgaagatac cgggcatcga
     6901 taccgtagaa cttgcacaaa ttttcttgcc gacggaacca agttttcgct tagctgattt
     6961 gtcagaaagc cttggcttta ttcatgagaa tcctcatcag gcggatagtg atgccgaagt
     7021 gacaggtcag cttcttttac tgatcgaaga acgaatgagg aaattgccga tcatcacatt
     7081 agagcagatt gcacgattaa gtagccatac aggaatggat acgagtcgtt tcatttacca
     7141 cgtgcttgaa gaaatgaaag aaaacccgga gccccttgat ctctcattag aaatcatcga
     7201 tgggctagct ttgcagaaaa aagaagtgga gctattcaca tccgtccatt atggcgagcg
     7261 gacatatcca agaaaaaaac aagcaaaaga aaaaatgttt ggtaaaacac tgatgtatcg
     7321 aaaagaacag aacaggctga tgaatgctgt ctacgaccat ttcacaaaag atgaatcaaa
     7381 agatttgatg atcgaagcag ctacaggaat gggcaaaacg attggttatc tgttgccttt
     7441 gagttatttg gctacacctg aaaaaccagc tgtcatcagc accgtttcgc ttgttttgca
     7501 gcagcagatc atggaaaaag acattccatt actgaatcaa ttgctagatc agccgataca
     7561 acctgtgatt atcaaaagct atcgccacta tattgattta cagcgtttta aaggtacact
     7621 tgtggaaccg ccgggacaaa aacaatatgc tttgtaccaa atggctgtct tagtttggct
     7681 aacacaaacg aagaccggtg atttggatga attacattta accaatctga accatgcatt
     7741 ctttgcagat gtcgcgcaca gaggcactgg gtttttagca aggaatcagt cattttacaa
     7801 acaagacttt gtgcgtttct tgcaaaagaa aatcaaacaa agcaattttt tgatagtcaa
     7861 ccatgcattt ttagtacaag aaacacagcg aaaagaacca ttgcttccag ctagtcctta
     7921 tttgttgatt gatgaagtgc atcatttgcc ggaaattgct gaaaaagtaa atgacaaacg
     7981 actaagtatt aactacttcc ataaacaaat tcagcaactg gaagagccga atcagttatt
     8041 tgaccgcata caaagttatt tgccgaagga acatgaagcc ttgcgctggt tgtcgattta
     8101 tcgagaagaa ctctctgcac tatgcgaagc gcagatttgc gtggcagagg gattaatgaa
     8161 cttagtaagc agcaagcaac ctaacggaca atatccagaa gaagtcatga tgacaaaaga
     8221 aatgttggat cagctgccac tggaatctgg aacagcaatg gatcatctgc aactttttta
     8281 tcaagagatc caacaactac aatcacagat gcgcttgact tttgagatta tccaagaaag
     8341 ttggaccaac cagatgcgga tcgatttagc aagtttatat agcttatttg atcaattaga
     8401 caagcaatac ttgctgatgg accaatggct agaagactgg cgagatcatt tgatccattg
     8461 gttcgttccc gggaatacag cacagcagat gatttttcat ctccttgatt ttcaagcagc
     8521 tatgttgccg tcaactgtgt ggtatcctcg atatgaccat attctttata ttggaggaac
     8581 actgaagatc ggacctaatc gacagtatct ctctaaacga cttggcattg aagcagcacc
     8641 tcttaaagtg attgcttctc cttatgatta tgagaaacag gcaagaatct atgtgccgaa
     8701 agaagcacct gcagtccatc aagtcagcaa tcaagcatat atcgattatt tagaagcagc
     8761 actcacagga ttgattgaac aagaagatcg gccgatcctt gttttattca cttctcatga
     8821 tgttttgcaa aaggtatatc agaaaatgca tttgtctatg ctagaaaaag gacgtgaaat
     8881 tcttgctcaa ggatttggcg ggagtcggga aaaacttttg aaacgcttca ttttgtcaga
     8941 aaaaagcgtc ttatttggtg ctgacagctt ttgggaaggc gtagatttac ctggagatgc
     9001 tttgcagatt gttgtagtca cacgtttacc cttcgacaac ccgttacgtc cgatggtcaa
     9061 agctaggaat gcttatctgg aatcagaggg gatcaatcct ttttatcagg aatccgtgcc
     9121 taaagcagct ttgaagttac gccaagcatt aggtcgtttg ataaggacgg agacagatcg
     9181 tggcgtatta cttattttgg atcggagatt agtcactgca aaatatggtg cacgaatcat
     9241 ccatgcatta ccgaaaaaag taccgataaa agagctagct atagatgaaa tctatcagga
     9301 tatccatgaa ttcttaaaga aaaatgaaga aggaagttaa aggtgccgtc ttttttcatc
     9361 attaaaatct gctataatga aaaggaatga agataaagga gcgacgaccc ttgaagaaaa
     9421 aacagacaaa ggaaaaaacg attaatcgcg tgttgcttgg cttgatcgta tttttattag
     9481 cggtcatcgt gatcagctcg attttttact tgcgttccag tcgtccggcg gtccaggcga
     9541 aaaaagaagc agtagaaatc gcaaagaaat atgcaaatct cgaggaagcc gatcattttt
     9601 actggtttac tcgaaagaaa acgtactttt cattgacggg tacgaatgat aaaggaaaag
     9661 aaatcgccgt tatcattcct aaatctggtg aaaaagtaaa agtattggat caaacaaagg
     9721 gactgactga aaacgaagca aaacagacaa ttgctgcggc ccatcctgaa atccaaatcg
     9781 aaaaagctgc tttagggatg tatgatgatc agcctgtgtg ggaagtagta ggaaaagaaa
     9841 caaatgggaa actgaattat tacctgattg gctttgaatc tggagatgaa gtcaagacaa
     9901 tcaagggaat ttaaaaggta aaggaatgaa tgggatggag ttatcaaaaa gaacaaaacg
     9961 attagaaccg tctgtcactt tggcagctgc ggcaaaggcc aaggcattaa aagcaaaagg
    10021 cagagatgtg ctgagtttga ctgtgggaga accggatttt gcgacaccag aaaacatcca
    10081 agaagcagca atcgaagcaa tccgcaatgg gaaagctagc tattatacac caacagcagg
    10141 aatcccagag cttcgacagg caatcgttga ttatctgaag aaaaactatg gacttgtcta
    10201 tgaaccttca caaacagtcg taacagacgg agcgaaattt gctttgtata cccttttcca
    10261 aacgatcctt gacccgcaag atgaagtcat cattcctgtg ccttactggg tcagttacgg
    10321 tgagcaagta aaactggcag aaggacttcc tgtctttgtg aaaggggaag aatccaatag
    10381 ctggaaagtt actgttgagc agctagaaca agcacgttca gaaaaaacaa aagccgtaat
    10441 catcaattct ccatcgaatc cgactggaat gatttatagc aagaatgaac tgcaggcaat
    10501 cggtgaatgg gcagtaaaaa atgatatttt gatagttgct gatgatattt atggccgttt
    10561 agtttataat ggcaatgagt ttactccaat tgcaacgatt tcagaagcaa tcaaaaatca
    10621 gaccatcatc atcaatggcg tgtctaaaac atatgcgatg acaggctggc gcattggtta
    10681 tgcggtgggg aattctgaga tcatcaatgg aatgattgct gttgcttccc agtcaaccag
    10741 taatccgaca gcagtcagtc aatatgcagc tgttgaagca ttgaaaggcg agcaagatac
    10801 agtggaagaa atgcggaacg cctttgaaga acgcctgaat acgttatatc cgcttgtggc
    10861 agaactgccg ggtgtttctc ttgaaaagcc acaaggagct ttttatcttt ttcctaatgt
    10921 aaaagaaacg ctacgcatgt gtaaatatga aaatgtcaca caatgggtag aagatctttt
    10981 agaagaaaca ggggtagcgc ttgtgactgg cgaaggcttt ggggcaccag aaaatgtccg
    11041 catgagttat gccactgatc gcgctacgtt agaagaagcg gtacgcagaa tcaagcaatt
    11101 tattgagagt aaaagtcaaa attaatcctg aagaaattca aggaatataa aaggagagtc
    11161 atacgtggaa caaattcaaa tcatcgattc gaaaaaccat gtgggagaaa cagtaacaat
    11221 cggcgcttgg gtcgcaaata agcgatcaag cggaaaaatc gcatttttgc aattaagaga
    11281 cggaacagcc tttttccaag gtgtagttgt aaaaagcgaa gtgccggaag aagttttcca
    11341 aacagctaaa aatctacacc aagaaacatc cgtgttagta actggagaaa tccgtgaaga
    11401 cagtcgttca aaatttggtt atgaaatcgg aattacaaat gtggaagtgg taagcgagag
    11461 ccacgattat ccgatcacac caaaagaaca cggaacagac ttcttgatgg atcatcgtca
    11521 tttatggctg cgttcttcaa gacaacatgc gattatgcag atcagaaatg aaatcattcg
    11581 tgcaacgtat gaattcttta atcaacatca ttttgtcaaa atcgatccgc cgatcttgac
    11641 tggttctgca ccagaaggca ctacggatct attcgaaaca aattactttg atcaaaaagc
    11701 ttatttatct caaagtggcc aattatacat ggaagctgct gctatggctt tcggaaaagt
    11761 cttttcattc ggtccgactt tccgtgctga aaaatcaaaa acacgacgtc atttgatcga
    11821 attctggatg atcgaaccag aaatggcttt cactcatcaa gaagaaagtt tagaagtaca
    11881 ggaacaatat gtagcattct tagtacaaaa tgttttagat cattgcgaat acgcattaga
    11941 tgtactagga cgagacaaag aagtcttaaa aacttataca aaattgccat tccctcgtat
    12001 tacttatgat gatgcgatcg aattactgaa gaaaaatgga tttgatgaca tcgaatgggg
    12061 cgaagacttt ggttcacctc atgaaacgtt cattgcgaat tcattcgaac agcctgtctt
    12121 tatcctaaac tatccaaaag ccatcaagcc attctacatg aaaccacatc caacaagaga
    12181 agacgtggtg atctgtgcag atatgatcgc accagaaggt tacggtgaaa ttattggtgg
    12241 aagcgaacgg gcaacagact atgatttctt gctagaacaa atccgtaatc acggtttgga
    12301 tgaaaaagaa tattcatggt atcttgatct acgtaaatac ggttctgttc cgcactctgg
    12361 ttttggtcta ggccttgaac gaacagtcac ttggattgct ggtatcgacc atgtgcgtga
    12421 agctattcca ttcccacgtt tgttgaaccg aatttatcca taagaggttg tgaaatcagt
    12481 gccttgacct gagtattaga gaaacaaaac ggaaaaatag cttctcatat tttactgttt
    12541 tttgagctaa tatcgagggt cagctgattg aacaccgttt atatcaagag gttgtgcgtc
    12601 tgccgttcag ctccgagtca caaggagtaa tttcaaaaaa acattttttc atgtttgatg
    12661 actattgtga cttgtgacca aggagttggc cggagagcaa cgtttattta aaaaaaagaa
    12721 ctgcgaactc tcgcagttct ttttttttaa atacgggata aaggaagttt ccagcagata
    12781 agagaagaaa gcaaaagaaa ttgctatttg attcacaagg ttctataacg aaatcgcaat
    12841 caatcaaaca tatacaaaga aggttattca aatggaaatc tgatttgatg atgcttctgc
    12901 tgttgaaata aagcagcatc tagcacctga aaacaaacta atagtaacat tcgaagatgg
    12961 agtaggtccg tattttcagc atgcaatgat ccatatgtaa gttcaattca cccttgaaat
    13021 catcggacca gataaagtta tggccaatct atcccaagca atctagaaga gatattagta
    13081 aaggaacatt tactggaagt cttagatgag cctataaata tatgttctga tggcgtcatc
    13141 aagtggtctg attgacggaa gcttaggtgt gatcgatctc tctggaccaa acaatgaagg
    13201 aatcaagggg aatccagcac gttaagaaaa aaatagcata aaaaactaga cacttgatga
    13261 gcgttcattc aattgtttga agggactagg ctggtgtcat tttttataca tatattttct
    13321 ctttcgtctt gatagggctt atgggataat ataaggaaga aaatcatcgt tttattcaaa
    13381 aaggagtttt tgacaaatga aaaaacgtat attatttatc ggtgacagca ttacagacgc
    13441 aggaagagac cgagcagaag caactgatct aggtaaaggt tatccacttt tgattgccgg
    13501 aaaactaatc tcccaatatc caacgatgga atttgaatgc atgaaccgtg ggataggagg
    13561 agacaaaatt gcagatctgc aagtacgctg gcgagaagat tgtttagctt tacaacctga
    13621 ctatgtatcg attttgatcg gaatcaatga tacctggcat aatactagcg atcaggctat
    13681 attcggaact gagaaatctg ctcagcaatt tgaatccgtt tatcgaaaat tactaaatga
    13741 acttcaaaaa gcgggaatct gcaacatcat cttgatggag ccgtttgtac ttcctataac
    13801 cgacgaccgt catggctggc gtaaagactt agaccctaaa atccaaattg ttcgtaaact
    13861 tgctaaggaa tatggcgcat tgctggttcc tttagatggc atattcaatg ctttagggat
    13921 tgcttatggt ttttcgaaat atacaagaga agatggggtc cacccaacga ttggcggaca
    13981 tgagatcatt gcacaaagct ggatagatgc aattgccgca gtcgattttt ttagtggaga
    14041 taaaaatgag cgaaagaaaa gtgagaaatg atggaaatac ggtgggcagt aaaagaagat
    14101 ctacctcaaa tcaatcagct gattactgat catccagaat cattcgtaaa agcaaaaacg
    14161 gcagctgacc taaatgataa gaataaacta ttgctcgttg ctgtaacaaa agaaaaacgg
    14221 attatcggtt ttgcgcaagc ggacgtgcaa cagtcagaag aacgggtggg tcagcgacca
    14281 tcaggaaaac taatccaaca cacattagta acaaaagaaa atacggaaga aatccaaaaa
    14341 aaatttgcag aatatttgat gctggaagct aaaaaaagaa gaataaaatc aatttttgcc
    14401 cagttttaat caaaaacagt aatagaagga gaaatacaat gagttgttta ggaaatgtca
    14461 tctggtttat attcggcgga ctaatcggtg gtcttagctg gttagcagcg gggatacttt
    14521 ggtgtatcac gatcataggt atcccaatcg gtatgcaatg ttttaagatc gccttccttt
    14581 cttttgcgcc ttttggcaaa gaagtaattt atcaccagtc tggcatgtca ttgatcgtta
    14641 atatcatctg gctgatcgtt tcaggcctac caattgctct cgcacatgtc gtcagcggca
    14701 tccttctatg tatcacgatc atagggatac cttttgccaa gcaatctttc aaacttgctc
    14761 gattggcgtt gatgccattc ggtgcagaaa ttgtataaaa gagaataata aaggttagat
    14821 aaagcataga gtcaggtggg atacatgtat ttcacctgac tctatgcgtt ttcctgtata
    14881 agttcttcca tgtgactttt acgtctttta tttatatggg gttactgttt atggaaagtt
    14941 gaaataattt tcattaaaga actctttcta tatttttgaa agcgtagtgg agcttcgtat
    15001 atctgggaag gacattttgc agggattgaa aaaagatatt gtcctatcga aaaattaatg
    15061 aatttagaca aattgccagc aacaggcttc aaacttagtt gctatcctgt caagatcaag
    15121 aaggcaagtg cgggttggat aagagcagtg gcaatgatcg aagagaaaaa gaaagaataa
    15181 gccaaaacat ctgtatggaa gaaattttcc gatgcagatg tttttcattt tttctttcgg
    15241 ataccttgac atatgaataa ccattcattt ataattagtt atgaagagat attcatatgt
    15301 tgaggcgagg aggtcattaa aatgaaaagt tacagattag aaggtttaga ttgtgcgaac
    15361 tgtgcaatga aaatcgaaaa aggagttcag aaaatcaatg gagtcaagga agcaacggta
    15421 aacttcactt ctggaaaact aacgattgac gcggaggaag atcatttggc tacgattgaa
    15481 caagaaacaa aaaaagtagt gaaggaatta gaaccagatg tcaaagtaac agaaatagat
    15541 aaggaaaaag tatctgaaca tggaaatgaa aaagagagaa atacgctgtt ccggatttta
    15601 ttttcccttg caggtatcgc tttattgcta ttatttgatt ttaatgaact aattcgcttg
    15661 atagggtatt tattgattta tctgctgata ggatatgatg tagtgaagaa ggcagtgatg
    15721 aatatcgtca aaggaaaaat ctttgatgag aattttctta tgtctgttgc tacgattggc
    15781 gctatgttga tcggtgaata tccagaagcc gtagcagtga tgctatttta tcaaatcggc
    15841 gagtatttcc aaggattggc agtcagtcat tcaagaaaat cgattcgcga attgatggcg
    15901 atccgtccag aaacagctca cgtgcagacg gcggaaggat tgatgaccgt gaaccctgaa
    15961 gacgtgctga tcggtcagtt tgtacttgtg aagcctggtg aacgtgtacc gttagatgga
    16021 acgatcatcg aaggagaatc attagtcgat acgtcagcac ttacaggaga atcagtccct
    16081 aaatctgtat atgttggtga aacagtatta agcggattta tcaataaaaa taaaccattt
    16141 ttagttcaag tagaaaaatc ttatgaaaat tctactatca gcaaattatt agaattagta
    16201 gaaaatgcca gcagtaaaaa agcaccagct gaaaatttca ttactaaatt cgcgcgctat
    16261 tacacaccag tcgttgtagg attggccgtt ttattagctg ttttacctcc acttgttgtt
    16321 tctggagcgg cgttttctga atggatctat cgagcattga catttttagt gatctcatgt
    16381 ccatgtgcat tagtgatttc cgtgccactt agtttctttg gcggtatcgg aggagcaagt
    16441 aaaatcggtg tattagtaaa aggttcaaat tacttggagt tgcttgcgca aaccgagaca
    16501 gtggtattcg ataaaaccgg cacattaacg aaaggcgact tttcgatcca gacgatcgat
    16561 acaaaaatcg atccaaagat attcatgcaa tatattgcga gtgcagaaca gttttctaca
    16621 catccgattg ctcaatctgt tttagaaggc tacgcaggtc cgcttttacc aacagctaat
    16681 atccaggaat ttgcaggtga agggatctta gctgaagttg atggcaaaca agtattagta
    16741 ggtaatcaca aattgatgga aagatttgaa atctcttttc cttcaagtca agaaatcggt
    16801 acattgcttt atctagcaat cgatcaaagt tacagtggtt atcttgttat tgcagatacc
    16861 ttgaaagaag atgccgtaga cgcattggtt caattaaaac aggctggtgt gaaaaacaca
    16921 gtcatgttga cgggcgactc gaaaaaaatt gctgatcata tcgggaagca agtaggcgtg
    16981 gacaagatat atagcgaatt gcttccagaa gataaagtgc agcggttaga ggaaatctta
    17041 caaagtaaca ataaaaagac cgcatttgtc ggagatggaa tcaacgatgc accggtgcta
    17101 gcaagagcag atgtaggaat cgcaatgggt ggattgggaa gcgatgctgc aattgaagca
    17161 gcagatgtag tcatcatgaa tgatcaacca tcgaagattg cagaagcaat ccatttagca
    17221 aagaaaacat tgaaaatcgt caaacaaaac attgtctttg ctattgggat aaaaattttg
    17281 gtactttcct taggtgcttt tggttttgca tcaatgggag atgcagtatt tgcagatgtt
    17341 ggggtgacag tattagctgt tttgaatgcc atgcgaagct tgcatgtaaa aaataaacgt
    17401 tgatcaatga agaggacgaa tgaacgtcct ctttttttta tatccttttc ttaatgaaaa
    17461 atgtaaagaa aatagatagg attataaaat aactaatgtt tttaattttg cagaaaattc
    17521 aaaatagctt gaaattcaat aaggtcaatg aaataaaaga aaataactta tttttttgat
    17581 ggaaaaaata tgaaaaaacg atgtgtatta aataaaataa tacacacata aaataacact
    17641 gtcttgtaag tttgttaaaa attgaatatg attacatctg gataacaaat ttttatcata
    17701 aaggagagaa cgaatgaaca acaaacaatt acgctggttt actgttgcgt taattgcatt
    17761 taacatggtt tggggcatgg ggaatgtagt taataactat gctcagcaag gagtatcagt
    17821 tgtcacctct tggctattga ttttggccat ttattttatt ccatacgcat tgatcgttgg
    17881 acaattagga tcagccttca aggatagtaa aggaggcgtc agttcatggg tcgaaaatac
    17941 gacatctaat aaacgactgg catactatgc cgcttggact tactgggtcg tccatattcc
    18001 ttatttggca caaaagcctc aagcaattct gattgctggt ggttgggcag tccaaggaaa
    18061 tggaaatatc gtaaatacga tgtcagtaca aatggtagca gttatttcac ttattatatt
    18121 tcttgccttt ttatatttat caacaaaagg actttctact ttgaaagtga ttggcggact
    18181 ggccggaaca gcgatgttcg tcatgtcgat cttatttatt cttttagctg taacggcacc
    18241 gttcatcaat ccaacgatgg aatttgccac accacatatg aatcaagtga aaacatatat
    18301 tccaacattt gatttcagtt atttcacaac cgtttcgatg ctagtatttg cagttggagg
    18361 ggcggaaaaa atatcccctt atgtcaactc tacaagaaat cctgcaaagg aatttcctaa
    18421 agggatgatc ttcttagcag caatggtagg gatttgtgct gtattaggct cagtcgcaat
    18481 gggtatgctg tttgctagcg ataatatccc taaagacttg atggcaaatg gggcatacag
    18541 tgcttttcaa attttaggta attactatgg tatcggtaac tcattgatga tcatttacgc
    18601 attgacaaac atgattggac aaatctccgc attagccttt tcaattgatg caccactaaa
    18661 aatcttgtta gcagatgcgg atccagaatt tgtaccaaac tggctacgta aacgtaatcg
    18721 taaaggtaca ttggtgaatg gttatcttct gacaggtata ctagtaggaa tcattatttt
    18781 gttgccgatc tttggtatca aagatatgaa tgaattagta aaatggttga cgaacttgaa
    18841 ctcagttgtc atgccaatgc gttacctatg ggtgttcttg gcatatatga tgttgaataa
    18901 agcttataag aaattcaatt ctgaatataa atttgttaaa aatccaaaaa tcggttttat
    18961 ctttggtgct tggtgctttg ccttcactgc gtttgcatgt atcctaggaa tggtgccaaa
    19021 agtagattat actgctgatc ctaaagcatg gtggttccaa ttattcacga atatcatcac
    19081 accaattgtc ttgatcttat taggtatgat cttgccagca attgctcgtc gagacaaaaa
    19141 caaagaaatt actgaaccag aaacagtggt tcagccttaa aatagaaata cttgttacaa
    19201 gaggttgtga cagaagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgtc
    19261 ctgcatcaag taataagaac agccaaacaa aaatagctcc tcatattttg ccgttttttg
    19321 agctaatgct gaaggtcagc tgattgaaca ccatttattc gaaacaaggg actttgacac
    19381 gacttttgtc acagtccctt gtttttttgc ggcgttataa gtggtatgat cgaaccaaat
    19441 tccaaagaga agtgagggag aagaatgaag atcatcgtga ttggacaaag tgggtcggga
    19501 aaatcgtccc tcgcaaggaa gatcaaagaa atcaccggct ttccgcattc aggcaaaaaa
    19561 gtggtttttg caagaacagc aatcgtttat agtatctaat ccgtcatgga tcgttgaagg
    19621 aaattatact tctacgttag aggaaaggat taaagaagca gataagatca tttggcttaa
    19681 agtgccgcgt tatctcgcca tctaccgagt agtttggcgt tctctaaaaa gaaagataaa
    19741 caaaaaatct cggccagata tgcctgaatc attttcggag aaactaaaca ggaaatactt
    19801 agaattttta tcttttatct ggcattttga aggaaacaat gaaccaaaaa tacagcagct
    19861 gatccaagta gctaatgcaa gaaataaact gatcatcctt aaaacaagac gagagaaaca
    19921 agaattttta actaaattga aaatagagag caaaaataat tgacagtttt tgtttacaag
    19981 cgtaatctgt attcataagc taatgattac atttgtaaac gaaaggtgtg aaataaatgg
    20041 aaaaagtcaa aacgccagta aagatcagtg attcagagtg ggagatcatg cgtgttattt
    20101 ggacacttgg acagacgaca gcccaagaaa tcacgcagat cttagctgat tcgatggatt
    20161 ggaaaccagc aacgatcaaa actttgctgg gacgtttggt aaaaaaagaa gtgatctgga
    20221 cagaacaaaa aggaaaaaaa ttcatttatc atccagctgt ctcggagatg gaaacagttc
    20281 gcagtgcgac ggaaaatctt ttttcccata tctgtgcaaa aaggattgga gagacgattg
    20341 cggatctagt agaagaagct actttgactc aagaagatat caatgagatt ttgaaacaac
    20401 tggaacaaaa gcaaccagta gatacgatcg aatgtaactg tatcccaggt caatgtgaat
    20461 gtaaacaaca tcaataggag gaagaaaaaa tgaaacaaca attttcaatc gaaggtatga
    20521 gctgcaatca ttgtgcagca agagtagaag aaacagtaag cgcacttgac ggcgtgcaaa
    20581 aagtgaaagt aaatttgaaa aaagcaaacg gtacggtcaa gtttgatgaa acaaaagttc
    20641 aatccgaaaa gatttgtcaa gcaatcaatg gattgggtta taaagcagag gtgatttgat
    20701 ggcaacagca gaaaccaaaa tggatacgtt cgtcattaca ggtatgactt gtgcaaattg
    20761 ttctgctagg atcgaaaaag agctgaacgg acaacctggt gtggtcaatg ccacagtcaa
    20821 cttggccact gaaaaagcaa gtgtcaaata tgaagggacg accactgaaa aattaatcca
    20881 aagcgtagaa aacatcggtt atggtgcaat tttgtacgat gaagcccaca aacaaaaaat
    20941 tgcagaagaa aagcaagcgt atttgaaaaa aatgctattc gatcttatat tgagtacggt
    21001 tttgactttg ccgttgatgt tgtcaatgat tgctatgatg ttaggaagcc atgcggcgat
    21061 tgttcacttt tttcattttc caatcgtcca gttagtgctg tctgctcctg tccaatttta
    21121 tgtcggtgct cgtttttata aaggtgcgta ccatgcaatc aagacgaaag caccaaatat
    21181 ggatgtattg gtagcaattg gaacatcggc tgcctttgca ttgagcattt acaacggatt
    21241 tttccgtgga cacccccaag atctttattt tgaaagcagc agtatgatca tcacgctgat
    21301 tttgctcggc aaatatttgg aacatacagc aaaaacgaaa acaggtaatg caatcaaaca
    21361 attgatgtct cttcaaacaa aaaccgccca agtgataaga aacgggaaag aagaaacgtt
    21421 agcgattgaa gaagtagtcg ttggtgatca actagtcatc cgtcctggtg agcagattcc
    21481 agcagatggt cgaattattt caggaagcag tgcgatagat gaaagtatgc ttactggtga
    21541 aaatcttcct gtagaaaaga atccggatga cacgcttttt ggcgggacaa tcaatacgaa
    21601 tggtctgcta cacatggaag tcacacaagt cggcaagcag acagtattag cgcaaatcat
    21661 tcaaatggtg gaagatgctc aagggagcaa agccccaatc caaaaaatcg cagatcggat
    21721 ctcagggatt tttgttccga tcgtattagt catcgcgttt atcacgctca tagctacagg
    21781 tttgatcact ggtgattggc aactcgcatt gatccatagt gtctctgtat tagtcattgc
    21841 gtgtccatgt gcattaggtt tagcgacccc aacagctatt atggtaggaa cgggtgttgg
    21901 tgcacgaaat ggaattttaa ttaaaggcgg agaagcatta gaagcagctg ctcacttaga
    21961 cagcattgtt ttggataaaa ctggaacgat tacagaagga aagcccaaag ttacagatct
    22021 agtcggttca aaagaagtcc tatctatttt ttatacttta gaacaagctt ctgaacatcc
    22081 actaggtaaa gcaatcgtgg aatacggaaa actgcaagaa gcagcaacat acgatatgat
    22141 tgattttaca gcccacccag gtgctgggat cagcggtacg attaatggag tacgctattt
    22201 tgcgggtaca cgtaaacgat taattgaatt gaacctatca tttgatgaat accaagaaca
    22261 cgcgttagaa ttagaacagc agggaaaaac agtcatgttt ttagctgatg aaaagcaagt
    22321 gatcgggtgg attgccgtgg ctgatcaaat caaactagaa gccaaacaag caatcaagca
    22381 gctccagaat aaaggattag atgtttttat gctgacagga gacaacaaac tggcagccga
    22441 aacgattggt aaacaagttg gtattgatcc aaagcatatc tttgctgaag tacttccaga
    22501 ggataaagca gcctacgtag aaaaacttca gaaagatggc aaaaaagtcg gtatggcagg
    22561 cgatggaatc aatgatgcac cggccttagc attagctgat gtcggtatgg caatgggaag
    22621 tggaacggac attgcgatgg aaacagccga tgtgacgctg atgaatagca gtttggcatc
    22681 cattgcacaa acaatcgagt tgtctaggtt gacattgaga aaaatcaaac aaaatctttt
    22741 ctgggctttt gtctataata cgattggtat tccttttgcc gcactaggct ttttgaatcc
    22801 aatcattgca ggaggagcga tggcgtttag ttccgtcagt gtattgttga actccctgag
    22861 cttaaatcga cacatgaaaa agtaagagaa gggatggagt aaaaatgaat gggaacgata
    22921 tggagaaaaa acatgagcat aaaaaagaag tagtaaacaa agaaagtaca gaatctcatg
    22981 aaaaacagat ggagatgaaa catgaccata gtcagatgga tcattccatg cacatgggac
    23041 ataaccatgg agatatagca aacagcaaac aaatggacca ttcgatgcac atggatcacg
    23101 aacacggcgg catggatcat tccatgcaca tggggaattt caaacaaaaa ttttggctat
    23161 cgcttatcct agctatccca atcattcttt tttcaccaat gatgggaatg gaatttccat
    23221 ttcaagtaac gtttcctggt tctgattggc tcgtattgat cttagcaact atactattta
    23281 tctatggcgg tcagccattt ttaagtggag ctaagatgga attgaaacaa aaaagtcctg
    23341 ctatgatgac actgatcgcg atggggatta cagttgcata tatttatagt gtctattcgt
    23401 ttatcgcaaa tctgctcaat ccacatacac acgtcatgga tttcttctgg gaattggcta
    23461 ctttgatcgt tattatgcta ctaggtcact ggattgaaat gaatgctgtg tcgaatgcca
    23521 gcaatgcttt acaaaaacta gcagaattac tgccagaatc agtgaaacgc ttgacgaaag
    23581 atggaaaaga agagacagtt tcattgaaag aagtaaacga aggtgatcgt ttgatcgtcc
    23641 ggtctggtga taaaatgcca acagatggtg taatcttgaa aggtgaaaca attgttgacg
    23701 aatcagctgt gactggcgaa tcaagaggaa tcaaaaaaca agccaatgac aaagtgattg
    23761 gcggttctat caatggggat ggaacgatcg aaatcgaagt aactggaact ggtgaaaatg
    23821 gttatctagc gaaggttatg gaaatggtgc gaaaagcaca aggagaaaaa tccaagctag
    23881 agtcattatc tgacaaagtg gcaaaatggc tgttttatgt cgcattaatt gtgggtatcc
    23941 tggcattcat cgcctggttg ttcttgacag atcttcctaa tgcattagaa cgtatggtaa
    24001 cagtctttat catcgcctgt cctcatgctc taggtttagc tatcccatta gttgttgctc
    24061 gctccacatc aatagcagct aaaaacggat tacttttgaa aaaccgcaat gcactggagc
    24121 aagccaatga tctagatgtg atcatgctgg acaaaacagg aacactaaca gaaggaaaat
    24181 ttactgtaac gggtgtcgaa gtacttgatg atgcgttcaa taaaaatgaa atccttcaat
    24241 atctaggagc cttggaagcg aatgccaatc atccattggc tgttggcatt atgaattatt
    24301 tgaaagaaca tgaaatcaag ccttatcaag ctgaaaatct aaaaaatctt tcaggagtcg
    24361 ggctagaggc aacagtcaaa aaacaacaag tgaaaatagt taatgaaaaa gaagttgagc
    24421 gtctcggatt aaacgtagaa caagcattat tgaaacctta ccaagaacaa ggaaatacca
    24481 tcagcttcct gattttagaa aatcatctag ctgcaatcgt tgctttaggt gacgtagtaa
    24541 aaacagaagc aaaagaattt atccgaacat tgaaagaaag aaaaatcact cctgtcatgc
    24601 ttactggtga caataaaaac gctgcccaag cagtagcaga ttatttaggg atcgaagaat
    24661 attatggcgg tttattacca gatgataaag aagctgttgt ccagaaatac cttgaccaag
    24721 ggaaaaaagt aatcatggtg ggtgatggga tcaatgatgc gccaagttta gcccgtgctt
    24781 caatcgggat ggcaatcggt gcagggacag atatcgcaat cgattctgcg gatgtcgtat
    24841 tgacaaacag cgatccaaaa gatattttgc attttcttga tctagccaag caaacgcgaa
    24901 agaaaatgat ccagaacctc tggtgggggg caggttacaa tattcttgca attccgctag
    24961 cagccggaat tcttgcgcca attggtatca tattaagtcc agcagttggt gctgtcttga
    25021 tgtcgttaag tacggtagtt gtagctctaa atgctttgac attgaaaatc aaataataat
    25081 gaaaaaaggt tgtgacaaaa aacgtgtcac aacctttttt tcgagtaatc gatactcaat
    25141 cagctggttc tcggcattcc agaagtagat tctccagaaa ttttatctta ttttttgaag
    25201 cggctccctt ttgtcagaat ttttttgtat accgtttttc ttattatacg cgttatttct
    25261 aaataggttt ttttatcgaa ttttaccgga tctgcttgtc gtccatatat ttctgcttca
    25321 tattgagtag ttaatcgaat gaaacgcaga tctagcttga attcttgttc tattcgcagg
    25381 ctatattcct gcaaagattc tgcgggtttg cgcggtacca atgtttccaa agggttaaga
    25441 agtttttgat atgcaatcgt attagtagaa gcaaaaaaat gaagatagat aagataactt
    25501 gtccagcgca gagattttct gaataacaat agaaagaaca acaagacgga taaacacact
    25561 ccaaggctga ataagacgat tttataagct ggtgtgagtg ttgtttcttc tgatggaatg
    25621 acctcagatc tctcactgga aatagtttct gccatatttt cgctttcatt tggttgttcg
    25681 atagcaggct ggacggtttc ggttgtttca gcaactggtt gtgaacccat tggattggca
    25741 aatgaagggg ttggttcaaa tggaagccag ccaaatccag gaaagtatac ctctggccaa
    25801 gaatgagcat gactattgag aatgctgtat tcctcattcc cttgattatc tgtgcctgtt
    25861 ctgagcccat ttgtaaaacc ttttgtccag cgagctggga taccaagtgt tcgaagaagg
    25921 acgaccatag aagttgaaaa gttgtcacaa tagccgactt ttgaatcaaa gaggaaataa
    25981 tccacataat cgtgttcttt cggagtatgc ccggtatccg ttttggaata gcggaaattt
    26041 ccgcttgttt ttaaatactg ttagacagct tcgactttac gaattaaagt tgcttcattt
    26101 ttggtcaatt cgaccgccaa gtcctcaata cgtttaggca aactttttgg caattgaaga
    26161 tttgtctgct gattttctaa atcatcagct tttaactgac tgctttttaa ttcttctctc
    26221 gtaaaaacta acctttctgt ttgtaaagtt gcagaacgtg tcgattcatt cgggacagga
    26281 atgaatcgct ggctttcagg caaatagtaa agcgtttgtc cactagctaa tgaaaaatca
    26341 gtcaaggtag tatttccata aggataaggt aagaaagctt gtttttttag ggtcaatgta
    26401 atgttttctt tgtcttctat aacggtttgg tagctcgtga tgtcaacagg gaattcttgg
    26461 attggttgga tgggtgcgtc ctgatcttct atccatccag aaccggtgta atgattttta
    26521 ttttcgatct tccaatagcc atttcctttt tggttcgctg taaaaacaac ttcgccatta
    26581 tcatataaag gtccaccaag ctgttcatca ttctcactga agcctgttct tgcacttccg
    26641 gttaattgat actcatggag tgtgtcataa agcccttcat cattaagata ttctcggatc
    26701 ggttcactcg tttcctcgag agattggtgt actttcggaa gatataaagg caagatagcg
    26761 ctaaaagtta agaaacaaat aagcagaact gtagtcaatg actggaatcg agtgtttccc
    26821 gacaacaacc ggatggagaa aaattgcctt gctgccagat acacgaacaa aacagttact
    26881 accgcaatca tttcgtacag taaatctttt ttccgaaaaa cggtaagcat catgaagtaa
    26941 gccatcaaag aaagaaaggg tgcgtaccaa tagtcgtaat ccacggtcaa tgtaataaat
    27001 aaatagatca gcaacattat gagaaaaaaa gctgtctttt ctggcaaata ataaagttta
    27061 ccttgggata gatggaagaa ttcctctgta actgacgaaa aaaactgtaa gatccataat
    27121 atggaaaatg tcttcgaagg aaaatataaa aacaaacaac ctatcattac tggcaaagaa
    27181 agcagcaaac gtacccattt tttaggaata cagcagacaa taaggcatag gacaccaata
    27241 gttaggataa gaaggtagct gttttgcaat tgattagcta ctacaaatat cggagcacct
    27301 acacttagta tcacaaagga gtaaacgcca cgcagccatt tttcccgttt aatcgtcaat
    27361 ccattcacct cctggcaatg aacatatctt gtctttttct tggaccatga gttgtttttc
    27421 tacgaaataa acaagataag ccgttcgatc ttttgacaat gtttcaatag cttctgagac
    27481 ttcaggagaa ttctcaggaa caaagaggac gatttgtttt tttgcatatt ttttcaagtg
    27541 tgcgaaaaaa gctttctcat cgccagcttc caaagaagca aaataggtat gaccggggtc
    27601 tttgccatga aaggcgcggt tgcctaatat cagaagttcc ggctgctttt cttttctcaa
    27661 atgccataaa gaaaaataca agtctagcag aggttcaaat tttggactgg gcgaacccca
    27721 aaagcaaaaa agaggagaac attcttcttc ttgataatcg tattcacggt ataaccactc
    27781 ttgagtatga gcagacaatt tccaatcgat tcggttgaaa gaatcacctg gacgataagc
    27841 gcgatgttcc cgaaaatcaa gacctctttg gtacgcgttc agctgctgtt ttttttccag
    27901 aactctctgt aatttttctg cttgaatctt acaaaaagca ggcagaacag tcacaggaaa
    27961 agagatctct ctttttgcac tttttctgaa aagtcctaga ctatcatagg ctgtaaaaat
    28021 gacagttaat gtttcatggc atcctctttc ttttggctgc caaagcaatt ggacttcttg
    28081 cggtttattt gtaagaatca aaatctcttg tttttttctg caaagattgc tgggaagccg
    28141 atttgtaaac gagggagagg taaagaaaaa gttctctttt ttgctatgga aaaaataacc
    28201 tgcatacttt ttttgcgata aacgagtggc tgcaatgtac aagtaaccga aagtttcttt
    28261 agaaagctgc ttaatgaaat caagctccat gatacataaa gagtaaaaaa ggccaacaag
    28321 aaccagccta aagaattgtt gaatacaaga gcaaaaagca caatgatgaa atataccaga
    28381 aataacacaa aattaatgaa ataatctttg attttttcca ttgtctacct caatctacct
    28441 cacaggaata ggaatttgtg caacaagagt atgaatgatt tgtttctttt cgtcttcctt
    28501 caatgaacga tcgtaaaaaa tcaggcgatg ggaaaacacg taaggaacta aatcctgcaa
    28561 atctttgggc gtgacgtaat ttctcccatg gattaaagca taagcttttg ctgcctgaat
    28621 aaaggaaagg cttcctcgtg gactgatgcc aagctggatg gcagcatgac ttcttgttgc
    28681 tgacactaac tgcaaggcat aagatgccac ttcgggactg acaaagacat tcgccacatt
    28741 tgtttttatt tcttctattt cataactgtt caatacttca tttaagttat ccaacacttt
    28801 ttcgaattta tccaaaagta gcaaaagttc gtcatcaaaa gaaggataac cgatttgtag
    28861 tcggaataaa aaacgatcga gctgtgcttc cggcaaagga tatgttcctt cgtactcgat
    28921 tggattttgc gtagctaaga caaaaaaatg attgtctaga gggtatgtat tgttgtcgat
    28981 cgttgcatga ttctcggcca ttgcttctaa aagtgctgac tgagtacgag gagtcgtacg
    29041 attgatctca tctgccaaca agatcgtggt aaagatcggt cccggacgaa attcaaactc
    29101 ttttgattgt gaattgtata cggaaaaacc aagaatatca ctaggcagca gatcaggagt
    29161 acattggact ctgctgaagg ttccctgtac agcttttgct agtgctttga ccagtaacgt
    29221 tttacctaca ccgggaatat cttcgaataa gacatgacca cctgctaata gggccacgat
    29281 catcaattgg atagttgaac gtttgccgat gaccgctttt tctaattctt atatcaactg
    29341 atcgattttt tgtttggatt cttctatttt ttcttccatt ttcacatcac cttctcataa
    29401 aatcgctatt tatttcattg taatggaagg gaatacatcc tacaagcaaa gtgatcttat
    29461 tatgtttttt taaagtacat tcgttttgaa atgagaatac gattaaattg agtaaaataa
    29521 aaaataatga ggaagatttg cgtatttaaa taaatagaaa gggagttttt tatcttttta
    29581 ggagtgaact cattttgatc aaacaatatg tttagaaatc aggtgagaaa tgatcgatga
    29641 gatggcaaaa actattatca aaaaatgcac agaatttatt ttcactaatc aatctaatga
    29701 aagatttgat ctggcgaaaa gctgatttag ctgaagaact gaaaatcgat gttaaaacag
    29761 tggatcggca tctgaaacgt ttggcggaaa gcgactgtcc cattcagctg attcttcata
    29821 agaaaaccgt tcagttagtt tatgatcaag gatatagcga accttatctc ttgtttgttt
    29881 ttctgatcca atcacccccg ttttgtttac tgaaaaatct gctattaaat gaaccttacg
    29941 agcagaagta cgatcgttct acgcaagagc gattgagaaa gtggtttggc gagtaccacc
    30001 tttctttttc ttataaaaaa gcacagatct ttggttcaga aagaaaagta agatatctgc
    30061 tgttttactt tttgaaagat tttccgcatt tttttgcaga tgatggagag cagtcctttt
    30121 ctgagatgtt catccaatta ttgaagcaaa gaaaggatcc ggcagaaatc gagattttag
    30181 gagacagtcg aacactattc tttgaattgt ttcctgaatt gctatttaga gaggagcaga
    30241 ttttgccaat aaaagaaaaa aattatctag caattttgcg cttgagctgt aaccagatta
    30301 ctgaaaagcg gtggaaacgg atagaagaga cacaagacta tcggaaattg acgattgtga
    30361 ctgaaaagct tgattcgctg ttttggctga atagcgaatt tgagagcgaa agcaaatcaa
    30421 agctgataaa agtactttac caagaatggc ttcgttacgt aattggtatc catgaccggc
    30481 tgatagaaga agttcgttat tatcaggaaa cattagaaaa atttcccaat tccaaagata
    30541 aactatatca atttcggcta gcgatccaac aagcacttgc acaccccgca gtagagtggg
    30601 ggatgctttt actgaaaaca ctccaggaac gagggtattt agatgtccac cctcctgaag
    30661 tgaaaatcgt cttttttttt cgctatgaac accaagaagc aagaagactt gctggggtac
    30721 tgaacaaaac gctaagacat cgaaaacgga tctctataca tgtatgtact cgaaataagc
    30781 cgcctcggta tgcagatcta gtgatcaccg atcatttttt tccactagac cataacgaag
    30841 atcaaaaaac gtatctgtac catccttcat ttggtatcga aagacttctt accgatatca
    30901 attactggtt aggcaaaaac cgataaaacc tttggaaaaa agatcaaaac aagtcagtct
    30961 gtgctataat aaaaagcgat tagtaaagga ggaaatccat tggaattaaa gaaattagaa
    31021 gttcccacat cttcgatcac agaagtgaaa cgatcgccga tggatgtatt tgctcaagcg
    31081 cgagacgcgg gaactggcgt atatatattt aatcgtgaaa aagtagctgg cgtgatgctg
    31141 acccaagaac aatacaaaac gctcttgcaa gaactagatg tccttcgtca aacagtgaaa
    31201 gtggagagga aagaaccaaa agaaataaac gaagaaccta ttaacaaaac agcttccgtc
    31261 gtatccaatg ctgaagggat ggaagagctg gcccaaacat tacagcactc gctcattact
    31321 ggtgtgacga ttacagcaaa aaatctagat gaacgaatgg tagcactagg ttttattacg
    31381 aaaaagaccg gatttggcgg agtcgtggat atgcttagcg agttgatgga aactgggaaa
    31441 atcaactacc agttaagaag aaaaccgcaa aatcggacaa tcatagctga aatcatagga
    31501 gagcaaaaca gtcaatctca attgatggat aaaatcatca tcaagaaaat ttttctacat
    31561 gagctataag aaaaccatgt tcgcacttct aggcaagcca ggctttgaag accttgccaa
    31621 agaattgaac gaacgccttt aagataaggg tttttcgcta gatttaaaaa atcagtgata
    31681 gaaacatgtt cgcttataaa taaaattagt gatagtttta gtgatagtga gcttaaaaaa
    31741 gcactcaatt tgagtgcttt tttatttgct cttctatata gttttctagt tgattcattg
    31801 atcttttttt cacttcggga gaaatatggg catagacttg tgtagtggaa acatctttat
    31861 gacctaaaaa atctttgata tcttctaaag gtattcctgc ttgtctcgtt ctagctgaaa
    31921 aagcaaaaca taatgttggt gttttccttt cagcatatct atcagcaaga tgtagagtag
    31981 gacaacctcg tgagccaaaa gtcctgtgca aatcgagtta caacacacac atgataaaga
    32041 aacttttgaa aagaactata aagtatacgt tgaattggca agagattcag caatgaaata
    32101 tggtattcca ttaacattag acactcctta taaccaacca ggaatcaaat cgcatttatg
    32161 ggtaacacaa aatatttggg gcgatcatac agatccttac ggttatcttt ctgaaatggg
    32221 tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg ataatccaac
    32281 tacttcggag gataaaccag taattgatcc aactcgagca ggtgctgcaa attctacgct
    32341 gacagatgga acaaattacg cccacattga tcagttcgga gaaatcgaaa acgcaaactt
    32401 gcatgtagct ggatggcaca ttgctaatta taaatacgag tatattttca ttatggacta
    32461 caatactgga aaagaactag ctagagtaag agctgatggg atttatagac cagacgtaaa
    32521 tcaagcttat aatactttag gaaatgttgg ctatcatgta tctttcaata tgcgtgattt
    32581 ccctaataag aaagtatacg tcatgatgcg tgcgacaaac gatccagaag gaaatactaa
    32641 gggtggagca caagattttc atgacaagcg ttggtattta aatattccac aacgataaaa
    32701 aatccccccc tcttt
//
LOCUS       DAYYGY010000023        32549 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000023 DAYYGY010000000
VERSION     DAYYGY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.023"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHA52_001097"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHA52_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8939835.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACHA52_001098"
     CDS             499..861
                     /locus_tag="ACHA52_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8939836.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACHA52_001099"
     CDS             913..2595
                     /locus_tag="ACHA52_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8939837.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACHA52_001100"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACHA52_001100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8939838.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACHA52_001101"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACHA52_001101"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8939839.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACHA52_001102"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACHA52_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8939840.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="ACHA52_001103"
     CDS             6430..7434
                     /locus_tag="ACHA52_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939841.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="ACHA52_001104"
     CDS             7435..8379
                     /locus_tag="ACHA52_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939842.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /gene="opp4B"
                     /locus_tag="ACHA52_001105"
     CDS             8379..9341
                     /gene="opp4B"
                     /locus_tag="ACHA52_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8939843.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="ACHA52_001106"
     CDS             9368..10282
                     /locus_tag="ACHA52_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939844.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="ACHA52_001107"
     CDS             10308..12089
                     /locus_tag="ACHA52_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8939845.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /gene="rnc"
                     /locus_tag="ACHA52_001108"
     CDS             12437..13123
                     /gene="rnc"
                     /locus_tag="ACHA52_001108"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8939846.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="ACHA52_001109"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="ACHA52_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8939847.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="ACHA52_001110"
     CDS             16733..17542
                     /locus_tag="ACHA52_001110"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8939848.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="ACHA52_001111"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="ACHA52_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8939849.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="ACHA52_001112"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="ACHA52_001112"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8939850.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="ACHA52_001113"
     CDS             complement(21094..21546)
                     /locus_tag="ACHA52_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8939851.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="ACHA52_001114"
     CDS             21696..22646
                     /locus_tag="ACHA52_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8939852.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="ACHA52_001115"
     CDS             22639..23598
                     /locus_tag="ACHA52_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8939853.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="ACHA52_001116"
     CDS             23595..24350
                     /locus_tag="ACHA52_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939854.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="ACHA52_001117"
     CDS             24373..25326
                     /locus_tag="ACHA52_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8939855.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="ACHA52_001118"
     CDS             complement(25970..26266)
                     /locus_tag="ACHA52_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8939856.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="ACHA52_001119"
     CDS             26621..26977
                     /locus_tag="ACHA52_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8939857.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="ACHA52_001120"
     CDS             26987..27886
                     /locus_tag="ACHA52_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8939858.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28406..29134
                     /locus_tag="ACHA52_001121"
     CDS             28406..29134
                     /locus_tag="ACHA52_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8939859.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="ACHA52_001122"
     CDS             29279..31306
                     /locus_tag="ACHA52_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8939860.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="ACHA52_001123"
     CDS             31414..32511
                     /locus_tag="ACHA52_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8939861.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT        10858 a         5667 c         7001 g         9023 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaaggatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagg
//
LOCUS       DAYYGY010000024        31199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000024 DAYYGY010000000
VERSION     DAYYGY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.024"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHA52_001124"
     CDS             1..369
                     /locus_tag="ACHA52_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8939862.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACHA52_001125"
     CDS             549..1490
                     /locus_tag="ACHA52_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8939863.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACHA52_001126"
     CDS             1559..2290
                     /locus_tag="ACHA52_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8939864.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACHA52_001127"
     CDS             2434..4614
                     /locus_tag="ACHA52_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8939865.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACHA52_001128"
     CDS             4634..5116
                     /locus_tag="ACHA52_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8939866.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACHA52_001129"
     CDS             5132..5893
                     /locus_tag="ACHA52_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8939867.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACHA52_001130"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACHA52_001130"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8939868.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACHA52_001131"
     CDS             7665..8150
                     /locus_tag="ACHA52_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8939869.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACHA52_001132"
     CDS             8143..8826
                     /locus_tag="ACHA52_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8939870.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACHA52_001133"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACHA52_001133"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8939871.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACHA52_001134"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACHA52_001134"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8939872.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACHA52_001135"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACHA52_001135"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8939873.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACHA52_001136"
     CDS             13123..13455
                     /locus_tag="ACHA52_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8939874.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHA52_001137"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHA52_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8939875.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACHA52_001138"
     CDS             14281..15654
                     /locus_tag="ACHA52_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8939876.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHA52_001139"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHA52_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8939877.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACHA52_001140"
     CDS             16709..17149
                     /locus_tag="ACHA52_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8939878.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="ACHA52_001141"
     CDS             17293..17994
                     /locus_tag="ACHA52_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8939879.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="ACHA52_001142"
     CDS             17991..19112
                     /locus_tag="ACHA52_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8939880.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="ACHA52_001143"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="ACHA52_001143"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8939881.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="ACHA52_001144"
     CDS             20491..21399
                     /locus_tag="ACHA52_001144"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8939882.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="ACHA52_001145"
     CDS             21434..21706
                     /locus_tag="ACHA52_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8939883.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="ACHA52_001146"
     CDS             21717..22763
                     /locus_tag="ACHA52_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8939884.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23014..25623
                     /gene="clpB"
                     /locus_tag="ACHA52_001147"
     CDS             23014..25623
                     /gene="clpB"
                     /locus_tag="ACHA52_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8939885.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25774..26460)
                     /locus_tag="ACHA52_001148"
     CDS             complement(25774..26460)
                     /locus_tag="ACHA52_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939886.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26438..26932)
                     /locus_tag="ACHA52_001149"
     CDS             complement(26438..26932)
                     /locus_tag="ACHA52_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8939887.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26952..28172)
                     /locus_tag="ACHA52_001150"
     CDS             complement(26952..28172)
                     /locus_tag="ACHA52_001150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8939888.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28319..30154
                     /gene="lepA"
                     /locus_tag="ACHA52_001151"
     CDS             28319..30154
                     /gene="lepA"
                     /locus_tag="ACHA52_001151"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8939889.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30350..>31199
                     /locus_tag="ACHA52_001152"
     CDS             30350..>31199
                     /locus_tag="ACHA52_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8939890.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTN"
BASE COUNT        10536 a         5486 c         6700 g         8477 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
    22921 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
    22981 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
    23041 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
    23101 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
    23161 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
    23221 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
    23281 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
    23341 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
    23401 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
    23461 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
    23521 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
    23581 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
    23641 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
    23701 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
    23761 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
    23821 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
    23881 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
    23941 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
    24001 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
    24061 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
    24121 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
    24181 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
    24241 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
    24301 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
    24361 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
    24421 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
    24481 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
    24541 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
    24601 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
    24661 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
    24721 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
    24781 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
    24841 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
    24901 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
    24961 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
    25021 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
    25081 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
    25141 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
    25201 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
    25261 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
    25321 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
    25381 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
    25441 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
    25501 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
    25561 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
    25621 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
    25681 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
    25741 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
    25801 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
    25861 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
    25921 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
    25981 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
    26041 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
    26101 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
    26161 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
    26221 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
    26281 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
    26341 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
    26401 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
    26461 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
    26521 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
    26581 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
    26641 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
    26701 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
    26761 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
    26821 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
    26881 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
    26941 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
    27001 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
    27061 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
    27121 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
    27181 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
    27241 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
    27301 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
    27361 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
    27421 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
    27481 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
    27541 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
    27601 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
    27661 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
    27721 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
    27781 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
    27841 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
    27901 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
    27961 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
    28021 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
    28081 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
    28141 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
    28201 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
    28261 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
    28321 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
    28381 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
    28441 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
    28501 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
    28561 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
    28621 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
    28681 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
    28741 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
    28801 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
    28861 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
    28921 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
    28981 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
    29041 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
    29101 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
    29161 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
    29221 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
    29281 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
    29341 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
    29401 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
    29461 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
    29521 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
    29581 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
    29641 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
    29701 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
    29761 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
    29821 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
    29881 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
    29941 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
    30001 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
    30061 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
    30121 tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa aaacctatta
    30181 aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga ttttaaactg
    30241 tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt cttgaagatt
    30301 tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca tggttgataa
    30361 ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc aaaaaaagtt
    30421 agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa
    30481 aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa ctctaataaa
    30541 agatgaggaa gataagattg agattctcat cggtaaacta attgcataca gagatatgaa
    30601 taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat tactcagtta
    30661 tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct
    30721 attaagtaat gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga tatttagttt
    30781 atcaaaagac gataaaattg ttatgggaaa aagaatacga gaagtccgaa agaaccggga
    30841 agagacgctt gaaaaatttg gtaaaaactt taccatctat acaggcaaaa atgttataag
    30901 tagatgggaa aaaggtatta atattccaga tattgaaaaa ctaatgaata ttgcctatct
    30961 tggtaaggtc actgttcctt accttatgta cggagaagat tataagaata tacttccaaa
    31021 agatgaaaga gttagcgatt ttaaaaagat aaattctttt agtatgggat taaggatgag
    31081 gaaaattagg aaagattatt atctagaaag ggaggagttt ggaaagcttt tttctccttc
    31141 aatttcaaaa tggtccatag ataggtatga gaatggtaga gatataccca atactaatc
//
LOCUS       DAYYGY010000025        31089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000025 DAYYGY010000000
VERSION     DAYYGY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.025"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..729)
                     /locus_tag="ACHA52_001153"
     CDS             complement(19..729)
                     /locus_tag="ACHA52_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8939891.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACHA52_001154"
     CDS             complement(726..2198)
                     /locus_tag="ACHA52_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8939892.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACHA52_001155"
     CDS             2336..2809
                     /locus_tag="ACHA52_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8939893.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACHA52_001156"
     CDS             complement(2962..3513)
                     /locus_tag="ACHA52_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8939894.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACHA52_001157"
     CDS             3618..4148
                     /locus_tag="ACHA52_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8939895.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACHA52_001158"
     CDS             complement(4631..5074)
                     /locus_tag="ACHA52_001158"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8939896.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACHA52_001159"
     CDS             complement(5090..6250)
                     /locus_tag="ACHA52_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8939897.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACHA52_001160"
     CDS             6757..8457
                     /locus_tag="ACHA52_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939898.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACHA52_001161"
     CDS             complement(8559..9392)
                     /locus_tag="ACHA52_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8939899.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACHA52_001162"
     CDS             complement(9696..11861)
                     /locus_tag="ACHA52_001162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8939900.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACHA52_001163"
     CDS             12121..14415
                     /locus_tag="ACHA52_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8939901.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACHA52_001164"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACHA52_001164"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8939902.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACHA52_001165"
     CDS             15066..16073
                     /locus_tag="ACHA52_001165"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8939903.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACHA52_001166"
     CDS             16095..17114
                     /locus_tag="ACHA52_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8939904.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACHA52_001167"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACHA52_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8939905.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACHA52_001168"
     CDS             complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACHA52_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8939906.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACHA52_001169"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACHA52_001169"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8939907.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACHA52_001170"
     CDS             complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACHA52_001170"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8939908.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="ACHA52_001171"
     CDS             24539..25840
                     /locus_tag="ACHA52_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8939909.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="ACHA52_001172"
     CDS             complement(25909..26508)
                     /locus_tag="ACHA52_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8939910.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="ACHA52_001173"
     CDS             26762..27643
                     /locus_tag="ACHA52_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8939911.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="ACHA52_001174"
     CDS             27656..29266
                     /locus_tag="ACHA52_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939912.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="ACHA52_001175"
     CDS             complement(29343..30002)
                     /locus_tag="ACHA52_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8939913.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="ACHA52_001176"
     CDS             complement(30285..30950)
                     /locus_tag="ACHA52_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8939914.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         9296 a         6341 c         5644 g         9808 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gagagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttt
//
LOCUS       DAYYGY010000026        30801 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000026 DAYYGY010000000
VERSION     DAYYGY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.026"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..320)
                     /locus_tag="ACHA52_001177"
     CDS             complement(147..320)
                     /locus_tag="ACHA52_001177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939915.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            606..1934
                     /locus_tag="ACHA52_001178"
     CDS             606..1934
                     /locus_tag="ACHA52_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8939916.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2136..2969
                     /locus_tag="ACHA52_001179"
     CDS             2136..2969
                     /locus_tag="ACHA52_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8939917.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2985..3722
                     /locus_tag="ACHA52_001180"
     CDS             2985..3722
                     /locus_tag="ACHA52_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8939918.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3877..4845)
                     /locus_tag="ACHA52_001181"
     CDS             complement(3877..4845)
                     /locus_tag="ACHA52_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8939919.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5077..5910
                     /locus_tag="ACHA52_001182"
     CDS             5077..5910
                     /locus_tag="ACHA52_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8939920.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5944..6783
                     /locus_tag="ACHA52_001183"
     CDS             5944..6783
                     /locus_tag="ACHA52_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8939921.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6809..7369
                     /locus_tag="ACHA52_001184"
     CDS             6809..7369
                     /locus_tag="ACHA52_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8939922.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7568..9289
                     /locus_tag="ACHA52_001185"
     CDS             7568..9289
                     /locus_tag="ACHA52_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8939923.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9454..10596
                     /locus_tag="ACHA52_001186"
     CDS             9454..10596
                     /locus_tag="ACHA52_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8939924.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10612..11823
                     /gene="thiI"
                     /locus_tag="ACHA52_001187"
     CDS             10612..11823
                     /gene="thiI"
                     /locus_tag="ACHA52_001187"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8939925.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12396..13043
                     /locus_tag="ACHA52_001188"
     CDS             12396..13043
                     /locus_tag="ACHA52_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8939926.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13172..13388
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13436..16081
                     /locus_tag="ACHA52_001189"
     CDS             13436..16081
                     /locus_tag="ACHA52_001189"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8939927.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16391..17704
                     /locus_tag="ACHA52_001190"
     CDS             16391..17704
                     /locus_tag="ACHA52_001190"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8939928.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17694..18347
                     /locus_tag="ACHA52_001191"
     CDS             17694..18347
                     /locus_tag="ACHA52_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8939929.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18402..19094
                     /gene="radC"
                     /locus_tag="ACHA52_001192"
     CDS             18402..19094
                     /gene="radC"
                     /locus_tag="ACHA52_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8939930.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19244..19444
                     /locus_tag="ACHA52_001193"
     CDS             19244..19444
                     /locus_tag="ACHA52_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8939931.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19698..21377)
                     /locus_tag="ACHA52_001194"
     CDS             complement(19698..21377)
                     /locus_tag="ACHA52_001194"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8939932.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21379..21591)
                     /locus_tag="ACHA52_001195"
     CDS             complement(21379..21591)
                     /locus_tag="ACHA52_001195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8939933.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21984..23714
                     /gene="efrA"
                     /locus_tag="ACHA52_001196"
     CDS             21984..23714
                     /gene="efrA"
                     /locus_tag="ACHA52_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8939934.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23711..25471
                     /gene="efrB"
                     /locus_tag="ACHA52_001197"
     CDS             23711..25471
                     /gene="efrB"
                     /locus_tag="ACHA52_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8939935.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25566..25763
                     /locus_tag="ACHA52_001198"
     CDS             25566..25763
                     /locus_tag="ACHA52_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939936.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25916..26401
                     /locus_tag="ACHA52_001199"
     CDS             25916..26401
                     /locus_tag="ACHA52_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8939937.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26505..27098)
                     /gene="clpP"
                     /locus_tag="ACHA52_001200"
     CDS             complement(26505..27098)
                     /gene="clpP"
                     /locus_tag="ACHA52_001200"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8939938.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27278..27805
                     /locus_tag="ACHA52_001201"
     CDS             27278..27805
                     /locus_tag="ACHA52_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939939.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27896..28633
                     /locus_tag="ACHA52_001202"
     CDS             27896..28633
                     /locus_tag="ACHA52_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8939940.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28637..29554
                     /gene="miaA"
                     /locus_tag="ACHA52_001203"
     CDS             28637..29554
                     /gene="miaA"
                     /locus_tag="ACHA52_001203"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8939941.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29556..30785
                     /gene="hflX"
                     /locus_tag="ACHA52_001204"
     CDS             29556..30785
                     /gene="hflX"
                     /locus_tag="ACHA52_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8939942.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10173 a         5269 c         6572 g         8787 t
ORIGIN      
        1 ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt
       61 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
      121 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      181 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      241 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      301 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      361 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      421 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      481 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      541 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      601 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      661 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      721 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      781 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      841 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      901 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      961 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
     1021 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1081 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1141 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1201 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1261 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1321 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1381 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1441 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1501 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1561 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1621 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1681 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1741 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1801 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1861 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1921 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1981 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     2041 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2101 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2161 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2221 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2281 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2341 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2401 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2461 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2521 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2581 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2641 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2701 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2761 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2821 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2881 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2941 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     3001 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3061 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3121 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3181 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3241 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3301 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3361 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3421 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3481 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3541 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3601 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3661 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3721 aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat
     3781 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
     3841 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
     3901 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
     3961 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
     4021 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
     4081 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
     4141 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
     4201 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
     4261 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
     4321 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
     4381 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
     4441 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
     4501 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
     4561 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
     4621 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
     4681 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
     4741 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     4801 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     4861 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     4921 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     4981 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     5041 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     5101 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     5161 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     5221 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     5281 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     5341 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     5401 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     5461 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     5521 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     5581 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     5641 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     5701 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     5761 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     5821 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     5881 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     5941 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     6001 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     6061 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     6121 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     6181 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     6241 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     6301 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     6361 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     6421 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     6481 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     6541 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     6601 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     6661 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     6721 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     6781 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     6841 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     6901 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     6961 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     7021 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     7081 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     7141 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     7201 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     7261 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     7321 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     7381 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     7441 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     7501 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     7561 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     7621 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     7681 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     7741 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     7801 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     7861 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     7921 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     7981 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     8041 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     8101 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     8161 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     8221 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     8281 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     8341 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     8401 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     8461 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     8521 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     8581 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     8641 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     8701 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     8761 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     8821 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     8881 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     8941 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     9001 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     9061 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     9121 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     9181 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     9241 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     9301 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     9361 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     9421 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     9481 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     9541 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
     9601 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
     9661 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
     9721 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
     9781 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
     9841 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
     9901 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
     9961 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
    10021 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
    10081 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
    10141 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
    10201 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
    10261 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
    10321 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
    10381 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
    10441 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
    10501 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
    10561 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
    10621 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
    10681 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
    10741 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
    10801 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
    10861 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
    10921 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
    10981 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
    11041 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
    11101 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
    11161 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
    11221 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
    11281 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
    11341 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
    11401 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
    11461 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
    11521 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
    11581 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
    11641 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
    11701 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
    11761 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
    11821 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
    11881 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
    11941 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
    12001 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
    12061 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
    12121 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
    12181 ggttgcgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    12241 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgttttatt
    12301 tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa acatgttaca
    12361 ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca gatacactgc
    12421 caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg ctggatgact
    12481 caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt ccttctgcaa
    12541 cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat ggctatgatg
    12601 tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa aaacgaatcg
    12661 cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt agaagaaatg
    12721 aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat cgagtaattg
    12781 atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca aaaaatgtga
    12841 cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat gaaattgtca
    12901 aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc ccagcttatg
    12961 tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac tttgatgaga
    13021 attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt gcgagaactc
    13081 gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca cgaccacttg
    13141 ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga ggagtaactg
    13201 gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac aaccagtgaa
    13261 ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg atcgttaact
    13321 cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt aaatcatacg
    13381 tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa acagcatgac
    13441 ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg gacgttatca
    13501 gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa aaccttattc
    13561 aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg cttgggatac
    13621 gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct
    13681 gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga agcttagaga
    13741 gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg
    13801 gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct tatctttaga
    13861 ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac gtaaagtttt
    13921 tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca actgggatcc
    13981 tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg aaggcgcctt
    14041 ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg cgacaacccg
    14101 tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg agcgttatca
    14161 agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc caatcattgc
    14221 agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc cagcacatga
    14281 tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga
    14341 tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt ttgctgctcg
    14401 taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg atacgatgat
    14461 ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc tgtctactca
    14521 atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag aaacagatca
    14581 agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga tggaaaatgt
    14641 ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg catggtatca
    14701 caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg aaaactgggt
    14761 acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt tctcaacaat
    14821 gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta gcactttagt
    14881 aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa gcttggaatt
    14941 tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg acgaacaagg
    15001 gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa tcgaaaagta
    15061 tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac aagatgtccg
    15121 cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct ggaatgcttc
    15181 acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt tatctggaga
    15241 aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg aacgagtaac
    15301 tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata actttatctg
    15361 ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg gagatgatgc
    15421 tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga tcctacgttt
    15481 attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc ctcatcaggg
    15541 aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg atgaaacagc
    15601 ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga
    15661 agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg atgctaaaat
    15721 cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt
    15781 gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc
    15841 agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca aacgattaga
    15901 aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac tttctaatga
    15961 acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga
    16021 ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata
    16081 agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc tgattgaaca
    16141 tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga gatgtgacat
    16201 aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg agggaggtgg
    16261 atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct gtttcttttc
    16321 tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa gagaaatggg
    16381 ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat taccatttgg
    16441 ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc atccagagag
    16501 aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg tagcttattt
    16561 gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac cttatatcga
    16621 aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg atttgatcca
    16681 gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag cagtagctgg
    16741 tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga tgaagaaagt
    16801 ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca atgtagctgt
    16861 accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac ttggagatac
    16921 aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac
    16981 aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag caaaaaatgc
    17041 accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg atgagatgtg
    17101 gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa ccactccgct
    17161 tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt ttgtttactg
    17221 tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga agaaatctcg
    17281 atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag atggtgccca
    17341 taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca aaggatatca
    17401 aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc tagaacgatt
    17461 gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa aagccatcga
    17521 attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat tgtggcaatt
    17581 cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg tgaccggttc
    17641 actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag atcatgaagt
    17701 ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta gtgtattacg
    17761 aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac ctgtatttaa
    17821 aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt ttctcggaat
    17881 ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg cttgaacgtt
    17941 tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct tttttagaag
    18001 aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt gaacttttat
    18061 tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa aatgttcaac
    18121 gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt acagcgaaga
    18181 aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct gcggctgaga
    18241 tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc aaaaaaacag
    18301 ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa tccatctctt
    18361 ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa cgattgatca
    18421 aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc gcggaagctt
    18481 tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga
    18541 tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta cgacaggcta
    18601 ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc gaaatcaaag
    18661 ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct ccaccggttc
    18721 gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac aaacaagaac
    18781 atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa acagtgttca
    18841 tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat gctgtacgtt
    18901 attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt attccatcgc
    18961 agcaggatat gaattttacc aaaaggatac aaaagtgtgg agaaatgatg gggatcactg
    19021 tactggacca cttgatcatc ggacgcaaac gatattttag tttgagagaa gaaggaatga
    19081 tggaagagaa gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca attatattgt
    19141 aattttgttt gggaatagaa gttgaaacat ctgttgcacc tctatcgtac acgagcaatg
    19201 ttacaattta aattagtgct tttggcacga ggaggatatt attatggaaa acggaacagt
    19261 aaaatggttc aacgctgaaa aaggatttgg atttatctca cgtgaagatg gaagcgacgt
    19321 attcgttcac ttctcagcta tccaaggtga cggattcaaa acattagaag aaggtcaagc
    19381 agtgactttc gacgttgaag aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc
    19441 ttaattttat ctgtaaatca aattgatggt agacatcaat ttttacgaga gaacaaagcg
    19501 agtaacaaaa atacctgcca caagagacaa tttctcttgt ggcaggtatt tttttgtatg
    19561 aaaaagtaaa taaatttaag aggctatgaa agagccacct tgtatcaagg aataagaaca
    19621 aacaaaaatg gcttctcatg tttttcattt gttttctctt atattcagga caaggtgctg
    19681 atttcataac ctcttattta ttcttcatct ggtgtcatta ccattggcaa aatcattgga
    19741 tgtctttctg tacgttcgaa taagaaaggc tgaagcgcgc cagtcatcgc ttctttcaat
    19801 ttatattctg aacagtcagg tccattcaaa cattctctta aagcgtggaa taagattcgc
    19861 tgagcttcat tgatcatttc tcctgattct cgcatgtaga caaatccacg agagagaata
    19921 tctggaccag ccaagatttc ttttttcttg atatccactg tcgcgacagc taatacaaga
    19981 ccttcttctg aaagcaggtg acggtcacgt aaaacgacat taccaatatc accgatcccg
    20041 ctaccatcga tatacacatc tccagccaca aaatgcccag caggacgcgc agaatcgcta
    20101 gttaacgcta aaacatcgcc attttccatg atgaagcagt tttctgcagg aacacctaca
    20161 tcttgtgcta gagaagcatg tactttcaac atgcggaatt ctccgtggac aggaataaaa
    20221 tattttggct tcatcaaacg aagcattaat ttttgttctt cttgtccacc gtgaccggaa
    20281 gtatggatgt tattgatttt tccgtgaatc acttctgcac cagcttcaga cagcaaattg
    20341 atcaaatgat tgacacttgt tgtattacca ggaataggtg aacttgaaaa gatcactgta
    20401 tcgtctggct ggatttggat ttgacggtga gtaccattcg cgatgcggct caacgcggcc
    20461 atcgcttctc cttgagaacc tgtacatagg atcatcgttt cattggcagg caagcgattg
    20521 atttcgtgtg catctacaaa tgtgccgtct ggaactttga tatatcctaa acgttgaccg
    20581 ttgacgatgg cattttccat gcttcgtccg aaaacagcga ttttacgacc agttgccaca
    20641 gcggcatctg ctgcttgctg caaacggaag atgttagaag caaagctggc aaaaatgata
    20701 cgtccttcga tttttgaaaa gatcttcaaa atagaatgcc caatcgtttt ttctgattta
    20761 gtaaatgttg gaatctcagc atttgtacta tcagataaaa ggcacaagac tccttcttcc
    20821 ccgatacgag ccattttatg caagtttgct ggttcgccaa caggagtgaa atcgaatttg
    20881 aagtctccag tcgctacgat atttccagaa ggtgttttca ccacgatccc taatgcatct
    20941 gggatactat gggtcgttct gaaaaagctg acagaagtct ttctaaaacg gatgatcgta
    21001 tcttcattga tctcatggag ctctgcatct cgaagcaagc catgctcatc taatttgttt
    21061 gtgatcaacg ctaatgcgag aggaccagca tagataggaa tatttgcttg tttcagcaag
    21121 tagggtattc cgccgatgtg gtcttcgtgc ccgtgagtaa tcaccaaggc ttttactttg
    21181 tgtaagtttt gtatgatgta gctgtagtcg ggaatgacat agtcaattcc aagtaaatcg
    21241 tcttccggga atttgatccc ggcatcaatc aaaatgattt catcttggaa ttgtacccca
    21301 tacgtatttt tcccgatttc acctaatcca ccaatagcaa agacgcctgt ctcgttattt
    21361 ttgatggaaa gtttcatatt aaaactccac taatgagaaa tcagcatttt ctttttcgta
    21421 ctctagatga cttccttcta gaagttgaac gaattcaata ttgtatgggg tattttcttc
    21481 cacacgttgg cgagcgtcta catcgctttt tgcctcaatg taaagagatt ttgtttcttc
    21541 ccgtttaggg ttgcgtactt ttgtttcttg ataataaact ttatagatca ttgttttgtc
    21601 tcctttatct gcgtaaagca gtgatttagc tagttgattt ttttagacaa cacaaaacga
    21661 ggggacaagc cacctggttt ttcatatgct ttgactagaa tactggattc gagtcaaaga
    21721 gacaagcctc gtttttgctt atctgttcgg atattttttc aaccgtacct tctgtataaa
    21781 aaatatacaa caattaattg ttattctagc ataaaacagc aaataagtat agaaatcaac
    21841 tagattgaaa gaaaaaatca atgtttatca tcccgctata atcataatga cttttttatg
    21901 agagaagtgt tctattaata ggctcgttgt gctatcattt ttagtaatga aaatgtaaca
    21961 aaagattaaa aggagatgag aaagtgaaac taatgtggca ttataccatg cgttataaga
    22021 aattcttgtt tttcaatttc atatgcgtat ttggttttat tttgattgaa ttaggacttc
    22081 ctacgatttt agcacgtatg atcgatgtag gaatccgcaa tgatgatttt gattatgtga
    22141 agcaacaagg gatatttatg atcgttatta ctgtaatcgg tattattttg aatatcttgt
    22201 taggttattt tggttcgagg attacgacta atatcgtagc agatattcga gatgatttgt
    22261 tcgaaaagat ccaaagttat tcgcatcagg aatacgaaaa tctaggcgta tcttcattga
    22321 tcactcgtac aacaaacgat gcttatcaga tcatgttgtt cctgcaaaat attttacgta
    22381 tcggttttat ggcaccaatg atgttcattg tcagtttgta catggtcatg cgtacaagtg
    22441 tgacactcag tttgtatgta gtaggtgcat tacctttact attattggca gtcgtagcga
    22501 ttgctaaggt gtctgaaccg ttatctaaaa agcaacaaaa aaacttagat aaaatcaaca
    22561 gtatcttgcg ggaaaatctt tcaggattgc gtgtgattcg tgcattcgtc aatgaaaagt
    22621 ttgaagaaaa acgttttgct gtagtcaatg agaattatgc ttctagttca aaaagcttat
    22681 tccgtttgat ggctgttgca caaccgggtt tttatttctt atttaatatc gtaatggtat
    22741 tgatcatctg gagcggggca gtgcaaatcg atgcaggcga gcttcgtgta ggagatttga
    22801 ttgcgtttat cgagtatata ttccatgcct tgttctcatt catgttattt gctagtgtgt
    22861 tcatgatgta tccaagagca gctgtttcag ctggacgtat ccaagaagcc ctagatgcca
    22921 aaccttcgat tgtagaagat ccaaatggta tatctgatac aaaaactaaa gggtatatcg
    22981 aatttcaaaa tgtaacattt gcttatccag gccattcgca agagccagtg atacgaaatg
    23041 tcagttttac agcctctcca ggagagaccg ttgcttttat tggaagtacc ggtagcggaa
    23101 aatctacgtt gatccaattg atccctcgct tttatgatgt gagtgaagga aagatcttga
    23161 tcgatggagt agacgtgaga gattatcggc taagcaagtt gcgagataag attgggttta
    23221 ttccgcaaaa agcgctgcta tttactggaa cgattgcaga taatttgcga tacgggaaag
    23281 aagatgctac gcaagaagaa atggaacgtg cagctgagat tgctcaagct tcagaattca
    23341 tttctcgaaa accagatgga tttaatgaac atttatcaga aggtggttcc aatttttcag
    23401 gtggacaaaa acaacgattg gcaatagcca gagccatcat acgccgtccg gaaatctata
    23461 tttttgatga cagcttttct gcattagatt atcaaacgga tgcgaagtta cgtgctcgtc
    23521 tgaaaaaaga aacgacagaa tctactgtat tgatcgtagc acaacgtgtg ggaacgatca
    23581 tgcatgcaga taagatcatc gtattaaatg aaggagaagt cgtcggtatg gggacgcatc
    23641 gggagttgtt ggaaaaatgc ccgatttatt atgatattgc cgcttctcaa ttatcaaagg
    23701 aggaattagc atgaaaaaaa ccttttcttc cttaaaacgt ttagctcgat atatcagacc
    23761 gtatcgtgag acttttcttt tagtgatcct atttacagtt ctgacagtgg ctttcaacgt
    23821 ggctttgcca tatgttacag gacttcctac tacagaaatc agccgtaata ttgccaatgg
    23881 cgaatcaatc aatttttctt atatattcaa atgtctcatt tggatcacag ttgttggaat
    23941 agggtattgt atttcgcaat tactttctgg tgttttgatg acaaatgtcg ttcaaagtgc
    24001 catgcatgat ttgagaagag acatagaaga aaaaatcaat cgtcttccgg tttcatattt
    24061 cgataaaaac cagcaaggaa atattttgtc tcgtgtgacg aatgatgtag atgcagtcag
    24121 cggtgcttta caacaagctt ttattggtat cgtaaatgcg atattgggaa ttacgttggc
    24181 tgcagcaatg atgttttata tccagccgat gatggcgctg atctctatga tcatgattcc
    24241 aacatcgatc tggatctcaa aacgagtgat caatgcttcg cagaaatatt ttcaaagcat
    24301 gcagaattct cttggtgagc taaacggcta cgtccaagaa aatatgacag gttttagtgt
    24361 attgaaagta tatggacgcg aaaaagaaac attggaagga ttcaaaaaag ttaatcacag
    24421 cttgaaatac tttgggtttc gtgcagcatt catttctggc ttgatgatgc ctctagttca
    24481 attaactgcc tatgctactt atattggtat ggctgttcta ggaagctttt atgttattac
    24541 tggggcgatc gttgtcggtc aactacaagc attcatccaa tatatttggc aaatcagtca
    24601 gccaatgggg aacgtgacac aattgtcttc agttttgcaa agtgcatcag cggcaacgaa
    24661 acgtgtattc gagattttag atgagccaga agaaaaagaa aacaaagtag atgcttcact
    24721 gccagaaacg attttaggag atgtgacatt cgatcatgtc gattttgcat atgatccgaa
    24781 gaaaccatta atccaagatt tgaattttga agtgaaagct ggtcaaaccg tagctgtggt
    24841 cggtccgacc ggtgccggca agacgacatt gatcaatcta ttgatgcgtt tttatgacgt
    24901 agacaaagga gcaataagga ttgatggaat cgacaccaaa tcaatgagtc gcagtgatgt
    24961 gcgttcgcta tttggtatgg tcttgcaaga tgcatggctt tatcaaggaa cgattgcaga
    25021 taatatccgc ttcggaaagc ttgatgcgac agattatgaa gtagttgatg ctgcgaaaac
    25081 agcaaacgta gatcatttta tccgaacgat gccagatgga tatgatatgg ccatcaattc
    25141 tgagggagac aatgtgtctt taggacaaaa acagttgctg acgattgccc gagcagtggt
    25201 ctccgatcct aaaatcttga ttttagatga agcaacaagt tcagtagaca cccgtttaga
    25261 agcgttgatt caaaaagcaa tggatcgcgt gatggaggga cggacaagtt tcgtcatcgc
    25321 tcaccgttta tcgacaatcc gtgatgcaga tctgattcta gtgatggatc aaggtcagat
    25381 cattgaaaaa ggaacgcatg agagtttatt ggcacaaggt ggattttatg aaaaactgta
    25441 taacagccaa tttgctgagg aagcagaata agtcatataa gaataaaaca gtttaaaact
    25501 tattttttgt tctctattct tgataatggt aaagtaaagt tatcaagaaa agtgaggaca
    25561 cggagatgga acagttttta aatgaaaaaa ttactgaagc caaaagagaa gttgatcgtt
    25621 taacgcacgt ctgtcagacg aagccttttg attcagctga tttttttgaa aatgagtttc
    25681 aaatcgaacg cttggcagca caaatagaag tgtacgaatt aattttaaaa aaagtaatag
    25741 aagaaaatat agatgtacaa taaaaacagg taaaacgtca gttaccagat actgtaaata
    25801 gacatctggc ctgagcaggt tttacctgtt ttttttctta tgaaataaat atatgctatg
    25861 atacctttat tcaataggat tagtttctac aaaaaatgaa aggagaaaaa taaatatgga
    25921 acggtttata gcatcaaatg ccacagtgat tggtgatgtg acattgtcag aagatgtgac
    25981 gatctggtat caagctgttt tacgcgggga cagtaattgg ataaaaatag gacaaagaac
    26041 gaatatccaa gatggtacaa tcatccatgt cgatcatgat gcacctgtag atattgcgga
    26101 gaatgtgacg gtaggacatc agtgcatgct acatggctgt acaatcgaaa aaggggcgct
    26161 gatcgggatg gggaccacga tattgaatca tgcggttatt ggtgagaata gtttgattgg
    26221 tgctggatca ttagtaacag aaggaaaagt tattcctcct aatgtcttgg cttttggtcg
    26281 cccagctaaa gtgatccgtc ctttgacaaa agaagaaata caaaaaaaca aagaaaacat
    26341 ccagcattat gtcgagatag gtcggatgca tgcgcaagga aaattcaagg agcaaagata
    26401 ggcagtagca tccataaaat aaaaaaggat ggtcagtttg agttgaccat ccttttttat
    26461 ttggtttaga taagagagct taaaaaagct tcctttgaaa cagattaatt taatgaagaa
    26521 ctgttttcca tgacttcatc gatcaatcca tatgcttttg cttgttcagc tgtcatatag
    26581 ttgtcacgat ctgtatcttt ttcaatcact tcaagcggtt gtccagtgcg ttcagctaaa
    26641 attttgttta agcgttcgcg tgtttgcaaa atatggcgtg ccgcaatttc gatttcagtt
    26701 gcttgccctt gagcgccgcc aagaggttga tggatcatga tctcagcatt tggtaaagca
    26761 aatcgtttac cttttgtacc agctgtcagc aagaagcttc ccattgaagc agccataccc
    26821 ataacaattg tttggacatc tgctttgaca aagttcattg tgtcgtaaat agccatacct
    26881 gctgtaactg aaccaccagg agaattgata taaagataga tatctttttc agaatcttga
    26941 gcgtctaaga aaagcaattg ggcgatgatc gaattagcaa gatcatctgt tacttggccg
    27001 cttaacatga tgatacggtc tttcaataaa cgtgagtaga tatcgtaagc tctttcacct
    27061 cgtgaagatt gttcaatgac tgtaggaatt aaattcataa tagagttcct ccttgatttc
    27121 gtttagaaat agtgtaccat aatttagttt ctaataccta aaatgtagta tacatttttg
    27181 gtcaaaaaag gtcaaataaa aaacttaggc aaattagtcg cataaacccg ctttatggta
    27241 tgataagcag agaaacaaaa caaggaggaa caacagattg atagttggta ttactggcct
    27301 tatcggagtg ctgacaggtg ctgtagttgt ctatatcggc atgatactgc aaatgcgtca
    27361 cttggaaaat aaagaacagc gtagacgtga attggaattg aaagtcaaag aaatcgagac
    27421 gctgaatcaa ttaaataaaa agatcaacga gatcttgcaa aaacggacaa cgctgatgga
    27481 agaatatgtc agctttgatg catttgatga ttgttatatc acgattgacg attttgcgta
    27541 tctccaatca ttttcagcgc aaaatagttt ttatctgcca aattatattt tagacgagct
    27601 atttaagaat atctcccatc gaaaagtgat tttatctcca gatgaaacaa tgaagattgg
    27661 tggatatgct tataaaggcg gacgcgtgat tttagagaat tttacggata gtctgactga
    27721 aatgatcaat gaaaaaaaag ctcagttgaa gactatgacg cgccagccga ttggtttctt
    27781 ctcgaaagat gatgtttgga gctgatattt tgaagcattg ccgttgcaca tgagagcgtg
    27841 gtaatgcttt ctttttttct taatgataca atagaactga acggagtgaa gaaagatgtt
    27901 acaaatctat gcccatcgcg gaagcagcgg cacccaccca gaaaatacgc ttcctgcatt
    27961 tgcagaagct gtacgagtag gtgcagatgg tatcgaacta gatgtccatc taagcaaaga
    28021 tggttatttg atcgttatgc acgacgaaga agtggaccgg acgactaacg gtaaagggct
    28081 gattcgagaa aaaacattgg aagaactcaa aaaattgaat gcagggggca aattcagcaa
    28141 agaatttcca gcagctaaag ttcctgaatt gagagaagtg ttaggactgc tcctccaaaa
    28201 aaattaccga gggatgctaa tgattgaaat taaaactgat cagtatgaat atcccggtat
    28261 cgaagaaaaa ttagccgaaa cattaaaaag tagagattgg ccttttcaac atgcatattg
    28321 cagctttcat ttggattctt tggaaaaaat cgcattactg gaaccggaga tacagctgga
    28381 cttattgatg agtacttctg ataaaaagat ccaactagca gcagaaaccc cttatataga
    28441 aggagtacat ccaaaaaata cttggttgtt cgaacatcca gtccaggcag ctgattttcc
    28501 aaaagcaatt cgtgcatgga cgattaacga tgaagaagaa atggccaaat gtcaagaact
    28561 caaaatagat ggaataataa ctgattttcc ggagaaagct cttcggtgga aacaatcaac
    28621 gaaaagtagg taaaatatgg aaaaagtatt agtgatcgtc ggtccgacag cagtcggtaa
    28681 aacagcgcta agtgtggaac tcgcacaact atttggagga gagatcatca gtggtgattc
    28741 attgcaaatc tataaaaatc tggatatcgg tacagcaaaa atcaaagaag aagagatgaa
    28801 taatgttccg catcatttga ttgatgtgat cgaacctact caacgttact cagccgctga
    28861 ttttcagcat tcaggtcgac aattgattac cgagattgcg aaccgagggc acctacctat
    28921 catagctggt ggtacgggtc tctatatcca atcccttcta tatgattatc agttaggagc
    28981 gacagaagaa gcgaatcctg agattagaaa aaagtatgaa acgttagctg aaacaattgg
    29041 ttcccaagca ttatggaatc agttgaaaga aaaagacccg gcagctgctg agaaaatcca
    29101 ctggaacaat caacgcaaag tgataagagc tctggaagtt ttagagacga caggacatag
    29161 tatcacggca cctaaagaag agccaaagca attgtatgat tacctcatga ttggattaga
    29221 tacgaagcgt agtattcttt atgaacgaat caatcaaaga gtcgatttga tgctagcaga
    29281 aggtttagta gcagaagcgc gtaaggtgta tgagcttgga gaagtccagg ccagtcaagg
    29341 tattggatat aaagaatttt tcccttattt tgaagggaaa gaaacattgg aagaagcaac
    29401 agaacaagtc aaactacatt cgagaagata cgcgaaacgt cagttgacgt ggtttcgtaa
    29461 ccgtttggat gcacagtggt ttgatcttat aagtgagcca aatcaaaaaa gtcagattga
    29521 gcaaacaatc aaaaaatggc tggaggcgga ataaaatgga agaaaaagtg atcatcgtag
    29581 gggtagaaac cgaagcaaac cagcgttact tttcagagtc aatggaagag ctagttcagc
    29641 tgacgaatac cgcatcagga gaagtcgtat tcacaattac acaaaagaga ccacaagtgg
    29701 atcggcaaac gattatcggt aaagggaaat tacaagaatt agttcaacaa gcggatgcgc
    29761 atgaagcgga tttgatcatc tttaatcatg agctaactcc gagacagagc caactgatta
    29821 ccgatgctgt tggtcttccg gtaattgatc gcgtgcagct tatcttagat atttttgcga
    29881 tgcgtgctcg ttcgaaagaa gggaagctgc aggttgaact tgctcagtta gaatacctgc
    29941 tgcctcgttt agtagggcaa gggaaaacat tatctcgtct tggcggtggg atcggaacaa
    30001 gagggccagg tgaaacaaag ttagaaacag accgccgaca tatacggaat aaaatttttg
    30061 ctgttaagaa agaattgaaa gaagtagaag ctcatcgaga gcgcaatcgc caaaaaagaa
    30121 aaaatagcga gatttttcag attggactta ttggctacac gaatgccggg aagtcaacta
    30181 tcttgaatct attgacacaa gcggatactt attcgaaaga tcaattgttt gcaacgttag
    30241 atccattgac taagagatgg cgttttgctg aaggatttga gattactgta acggataccg
    30301 tcgggttcat ccaagacctg cctacgcaac tgatcgatgc tttccattct acattagaag
    30361 aaagtcaaaa tatggatctt ttgctacatg tagtcgatgc tagttctcca gatcgtattt
    30421 tacaggagca gactgtctta aaattgatgg atgaactgaa tatgaaagaa atgccgatac
    30481 tgactgttta caataaggca gaccagatcg acccagcgat gttcacgcct acgctattcc
    30541 caaatgtatt gatttctact cagacgaaag aaggtaagat ggcgttgatc gaagcaatca
    30601 aacgtcagct gatggaacta atgacccctt atacactttc tgtgccaagc aacgaaggac
    30661 agaaattaag cgagttgaga agacaaacaa tggttttgga cgaagcgtac atggaagaaa
    30721 caaacgaata tgaagttaaa gggtttgcaa aaaccaattc aaaatggtta agaaaaaacc
    30781 aataaaattt tattttttta a
//
LOCUS       DAYYGY010000027        30118 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000027 DAYYGY010000000
VERSION     DAYYGY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.027"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..397
                     /locus_tag="ACHA52_001205"
     CDS             <1..397
                     /locus_tag="ACHA52_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939943.1"
                     /translation="VQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTA
                     VVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAG
                     EHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            411..812
                     /locus_tag="ACHA52_001206"
     CDS             411..812
                     /locus_tag="ACHA52_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939944.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            814..1185
                     /locus_tag="ACHA52_001207"
     CDS             814..1185
                     /locus_tag="ACHA52_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939945.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1221..1523)
                     /locus_tag="ACHA52_001208"
     CDS             complement(1221..1523)
                     /locus_tag="ACHA52_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8939946.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1781..1894)
                     /locus_tag="ACHA52_001209"
                     /pseudo
     CDS             complement(1781..1894)
                     /locus_tag="ACHA52_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2277..3509)
                     /locus_tag="ACHA52_001210"
     CDS             complement(2277..3509)
                     /locus_tag="ACHA52_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8939947.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3763..4332
                     /locus_tag="ACHA52_001211"
     CDS             3763..4332
                     /locus_tag="ACHA52_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8939948.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4510..4950)
                     /locus_tag="ACHA52_001212"
     CDS             complement(4510..4950)
                     /locus_tag="ACHA52_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8939949.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5108..5872
                     /gene="map"
                     /locus_tag="ACHA52_001213"
     CDS             5108..5872
                     /gene="map"
                     /locus_tag="ACHA52_001213"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8939950.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5904..6827
                     /locus_tag="ACHA52_001214"
     CDS             5904..6827
                     /locus_tag="ACHA52_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8939951.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6903..8042
                     /locus_tag="ACHA52_001215"
     CDS             6903..8042
                     /locus_tag="ACHA52_001215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8939952.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8035..8835
                     /locus_tag="ACHA52_001216"
     CDS             8035..8835
                     /locus_tag="ACHA52_001216"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8939953.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8835..9662
                     /locus_tag="ACHA52_001217"
     CDS             8835..9662
                     /locus_tag="ACHA52_001217"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8939954.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9667..10374
                     /locus_tag="ACHA52_001218"
     CDS             9667..10374
                     /locus_tag="ACHA52_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8939955.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            10474..11340
                     /gene="rfbA"
                     /locus_tag="ACHA52_001219"
     CDS             10474..11340
                     /gene="rfbA"
                     /locus_tag="ACHA52_001219"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8939956.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11354..11926
                     /gene="rfbC"
                     /locus_tag="ACHA52_001220"
     CDS             11354..11926
                     /gene="rfbC"
                     /locus_tag="ACHA52_001220"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8939957.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11948..12976
                     /gene="rfbB"
                     /locus_tag="ACHA52_001221"
     CDS             11948..12976
                     /gene="rfbB"
                     /locus_tag="ACHA52_001221"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8939958.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13074..13925
                     /gene="rfbD"
                     /locus_tag="ACHA52_001222"
     CDS             13074..13925
                     /gene="rfbD"
                     /locus_tag="ACHA52_001222"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8939959.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13960..15993
                     /locus_tag="ACHA52_001223"
     CDS             13960..15993
                     /locus_tag="ACHA52_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939960.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16037..17317
                     /locus_tag="ACHA52_001224"
     CDS             16037..17317
                     /locus_tag="ACHA52_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8939961.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17527..18333
                     /locus_tag="ACHA52_001225"
     CDS             17527..18333
                     /locus_tag="ACHA52_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8939962.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18345..19565
                     /locus_tag="ACHA52_001226"
     CDS             18345..19565
                     /locus_tag="ACHA52_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8939963.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19555..21141
                     /locus_tag="ACHA52_001227"
     CDS             19555..21141
                     /locus_tag="ACHA52_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8939964.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21180..23318
                     /locus_tag="ACHA52_001228"
     CDS             21180..23318
                     /locus_tag="ACHA52_001228"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8939965.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(23386..24345)
                     /locus_tag="ACHA52_001229"
     CDS             complement(23386..24345)
                     /locus_tag="ACHA52_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8939966.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            24680..26071
                     /locus_tag="ACHA52_001230"
     CDS             24680..26071
                     /locus_tag="ACHA52_001230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8939967.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            26130..26933
                     /locus_tag="ACHA52_001231"
     CDS             26130..26933
                     /locus_tag="ACHA52_001231"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8939968.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            26966..27820
                     /locus_tag="ACHA52_001232"
     CDS             26966..27820
                     /locus_tag="ACHA52_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFM8939969.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            27817..28518
                     /locus_tag="ACHA52_001233"
     CDS             27817..28518
                     /locus_tag="ACHA52_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFM8939970.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            28526..29593
                     /locus_tag="ACHA52_001234"
     CDS             28526..29593
                     /locus_tag="ACHA52_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8939971.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            29586..>30118
                     /locus_tag="ACHA52_001235"
     CDS             29586..>30118
                     /locus_tag="ACHA52_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8939972.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKT"
BASE COUNT         9758 a         4937 c         6102 g         9321 t
ORIGIN      
        1 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
       61 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      121 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      181 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      241 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      301 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      361 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      421 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      481 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      541 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      601 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      661 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
      721 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
      781 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
      841 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
      901 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
      961 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1021 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1081 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1141 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1201 tttgtttgtt atttttttat ttaatctttg tatctttctg gatcaacgaa agtatacttt
     1261 atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcac gatatcaaac
     1321 aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta
     1381 ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
     1441 ttccatactt tataaaaatc tttgataaat ctattcgaag ggtcaaattt aaaccataat
     1501 tgtgtttttc cttccatcag cataaaagtt cacctcaaaa agagtatacg aacgaatgtt
     1561 ctttttgtga aggcggaatt taatatactg aataatagaa aacaagtact tgtgccaaat
     1621 tatgtgccaa aaaaattcga attcaacaaa atgtaaacaa atagaaaacc ttctatatta
     1681 agatttcctg caataacaaa cacaacagaa gacgtgtaat agttagtcag gaacgtacaa
     1741 ataacccctg tatcctttgc ggtacagggg gttattttgt ataagtagac attgtagttt
     1801 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
     1861 aaatagatgc atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa
     1921 aattgattac taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat
     1981 agattcgaat taaacgattt cgattggctt ataccagtcc tatccaacga aagatatatc
     2041 gttcttaact ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga
     2101 gatgactttt tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc
     2161 aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa taaaggatct aagccaatag
     2221 cttagatcct ttattctatg ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa
     2281 gcccaatcgc catttcggaa aatcggtact gtagagccat cttcacggat tccgtcgata
     2341 tccatcttgt cagatcctac catgaaatct acgtgggttt gacttctatt caatccagct
     2401 tctgctaatt cttcatcgga catttctgta ccgcctttca cactgaaggc ataagcagaa
     2461 cctagtgcta agtgattcga tgcgttttca tcaaataatg tattgaaaaa gatgatgcca
     2521 gattgagaaa taggtgaagg gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct
     2581 tcatctgtat caagcagttt tgctaagaca tcttctcctt gttcggcaga aaaatcaaca
     2641 acttttccat ctttaaaagt aaatttcata cccgagatga tggtgccagc ataactcagt
     2701 ggctttgtac ttgaaatgta accatctact cgatgactgt caggtgctgt gaacacttct
     2761 tccgtcggca tatttgccat gaatttttct ccacgagcat tatagcttcc agcgccttcc
     2821 caaagatggt ttttaggcaa tccgataatg atatctgtac cgggagcagt atagtgaagg
     2881 gcagaaaatt gttctcgatt caattcttct gctttttttg ctaatttttc atcatgcttc
     2941 ttccatgcta agacagggtc ttcttcatag acacgagtcg ttttgaaaat ctgatcccat
     3001 aatgcctcaa cttgtttttc ttctggcagt tctgggaaga cttttgctgc ccattgttta
     3061 ccagccgcag ccacgacagt ccaactgact ttatttgctt gagttgcctt gcgtaaattc
     3121 attagtgctt tgccattcgc tgattgataa gacgctactc gatggctatc tacaccagca
     3181 aatgcatcgg gatcggcaga aacaacgctg atcctgcttg ctcctttttc aagccattca
     3241 tccgcttgat cgattttaga ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa
     3301 agaaattctc tttggatctg gtcgtcagtc cattgaacga taacctcagc ggctcctagt
     3361 ttataagctt cttgtgtgat caatcgagca aggggtgcct gttccacgct gatctgtaaa
     3421 acaactgtgt gtcctttttc tgtggctacg ccagtttctg caattaagcg tgcatatttt
     3481 tttagaagtt cattaaaatc agataacata ataaaaattc ctcctcacca ttttttctgg
     3541 taactattag catgatacca tttaattatg attcaaacaa tgaggcttta aataaaaaaa
     3601 ggaggttatt tttttaaggt taagtttcat gatatatgtt actacttgct atttcagctt
     3661 gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaac atgttacaat gaaattaatc
     3721 aatggtggaa gcgttataaa aaaattaagg agattaaaaa atatggcaag aaaaaagacg
     3781 attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca
     3841 cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt
     3901 gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta tgatcattta
     3961 caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat
     4021 tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt agaagaatat
     4081 ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc
     4141 gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg
     4201 atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa
     4261 cagattgaaa agatgatcca agacagtata gatgctatct tgaaattcaa agaaccaatc
     4321 gaaatcgatt gaatatactc aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa
     4381 aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc
     4441 catgaatgca atgaataaaa gagccataca gattgaataa atctggatgg ctcttttatt
     4501 gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc
     4561 ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt
     4621 tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc
     4681 agatccgcag acaccgtaaa tttttcccaa tagatccaat tcttgcaagt cttcataaaa
     4741 gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat
     4801 atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat
     4861 atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat
     4921 gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac
     4981 aattatgaat tataccaaaa catcattgaa aagaaaataa aacctaaaga cacctgggga
     5041 atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga
     5101 cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc
     5161 tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg
     5221 ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat
     5281 tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg
     5341 attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga
     5401 tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga
     5461 gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca
     5521 agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa
     5581 tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc
     5641 agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat
     5701 cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg
     5761 gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac
     5821 taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca
     5881 gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc
     5941 tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct
     6001 ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta
     6061 atattttacc gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga
     6121 ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt
     6181 caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca
     6241 atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga
     6301 tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa
     6361 ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac
     6421 ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat
     6481 ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga
     6541 tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta
     6601 tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga agttttattg
     6661 tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg
     6721 cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa
     6781 aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct
     6841 tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc
     6901 atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta ttatcactga
     6961 tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg
     7021 aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat
     7081 ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga
     7141 tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga
     7201 cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag
     7261 atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta
     7321 gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg
     7381 gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg
     7441 gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag
     7501 caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata
     7561 caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg
     7621 tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt
     7681 tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac
     7741 atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt
     7801 atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg
     7861 gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg
     7921 gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag
     7981 agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat
     8041 aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt
     8101 cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt
     8161 tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc
     8221 tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat
     8281 ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa
     8341 gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca
     8401 cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt
     8461 tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga
     8521 ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc
     8581 tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag
     8641 cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac
     8701 aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga
     8761 aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg
     8821 tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat
     8881 tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc
     8941 ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata
     9001 tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt
     9061 ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg
     9121 acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc
     9181 cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc
     9241 gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt
     9301 caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt
     9361 ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg
     9421 agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa
     9481 gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa
     9541 ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg
     9601 tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt
     9661 gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag
     9721 ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg
     9781 tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag
     9841 cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg
     9901 aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa
     9961 aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc
    10021 attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa
    10081 gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg
    10141 tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg
    10201 attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga
    10261 agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct
    10321 tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag
    10381 atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt
    10441 gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc
    10501 agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat
    10561 gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt
    10621 ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat
    10681 gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg
    10741 tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat
    10801 atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc
    10861 gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat
    10921 gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct
    10981 gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct
    11041 tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa
    11101 ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca
    11161 ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct
    11221 tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt
    11281 gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa
    11341 ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat
    11401 ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc
    11461 agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg
    11521 tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt
    11581 agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg
    11641 tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg
    11701 atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa
    11761 ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga
    11821 ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt
    11881 aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa
    11941 ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt
    12001 tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta
    12061 tgcaggaaac aaagagaact tggccggact tcctagcgat cgcgtagaat tggtcgtagg
    12121 agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca
    12181 ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac
    12241 gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca
    12301 tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca
    12361 tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata
    12421 ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt
    12481 acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt
    12541 tattcctcgc caagtaacga gtatcttaag cggtattcgt ccaaaattat atggcgaagg
    12601 aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt
    12661 gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa
    12721 gaccgtgatg gaattgatct tagaaatgat gggccaacca aaagacgcat acgaccatgt
    12781 aaacgaccgc gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga
    12841 gctaggttgg cagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg
    12901 gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc
    12961 caaaaatgga caataataca gaggaaattt cactgtatta atggaaaaac acatgtgagg
    13021 caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataac catatgtttt
    13081 tattaactgg aggaaatgga caattaggta cagaattgcg ccatttatta gatgaaaaaa
    13141 atctgccata tgtttcaaca gatgcaaaag acttggatat cacagatgct gagaaaacaa
    13201 tggcttatat tacagaatta aaacccgatg ttattttcca ttgcgcagct tacacagcag
    13261 tagataaagc agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa
    13321 gaaatgtagc acttgcagca aaagctgtga atgcacaatt cgtctatatc agtacagact
    13381 atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac
    13441 taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag
    13501 actattatat catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca
    13561 cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc
    13621 gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac
    13681 catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag
    13741 aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa
    13801 aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg
    13861 tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata
    13921 agtaatttcc tttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
    13981 gcacgacgaa aagaaaagcc aaaagcgaat caggcagatt cgaatttggt gcttgacctg
    14041 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
    14101 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
    14161 aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag
    14221 acaccttttt acctctcaca agctgaagag gacttttcgt tggtgaacga ggattattca
    14281 ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc
    14341 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
    14401 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
    14461 aagaggaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
    14521 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgatt
    14581 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
    14641 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
    14701 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
    14761 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
    14821 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
    14881 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
    14941 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
    15001 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
    15061 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
    15121 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
    15181 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
    15241 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
    15301 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat
    15361 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc
    15421 tggagacgct tgttttacct cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc
    15481 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
    15541 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
    15601 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
    15661 tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
    15721 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
    15781 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
    15841 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
    15901 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
    15961 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
    16021 attagtagaa aggagagtga agataattca agcaatcgta caaaaatttg tcaacaaaat
    16081 gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcaggggt
    16141 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
    16201 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
    16261 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
    16321 atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
    16381 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac
    16441 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
    16501 caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct
    16561 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgttc gaatcatcgg
    16621 tgggatagca gtcgttgcag cctttttagg tatttgggtc tgccaatcta gaggtgcatt
    16681 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
    16741 tcgtgcgcca tttattgcct acacggttac gattctttta gctttgccga tttcttatct
    16801 ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt
    16861 ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt
    16921 ccagcgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    16981 tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg gtcgcttaac
    17041 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    17101 gatgcagtca tttatggagg atacattgac ttcataccga tgggttccga ttatttatat
    17161 cctgttggct atggccgttc atcgatatga tgaaccagaa gttacggagg aattctatgt
    17221 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga
    17281 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata cgagagaggc
    17341 tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct tctgtaaaat
    17401 agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc
    17461 ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc gcttggagga
    17521 acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa cagaaaatta
    17581 ctgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt tttaggaatc
    17641 ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt atttggtgtt
    17701 ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt agttaccggg
    17761 atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa
    17821 tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac gacgaaaatc
    17881 ctatccaatc tgtacaccca tttattcttc attctgattg gcatagctgt cgcgcttata
    17941 aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt ttgcatgtgt
    18001 gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt acctgatatc
    18061 atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat attatggagc
    18121 cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct gtactatgtt
    18181 gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca tggaacatat
    18241 actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc agtgttccgt
    18301 agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca gaatatgcaa
    18361 ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg gatcgatttc
    18421 gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg ttgaaaaacg
    18481 tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa
    18541 aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc acgatggaaa
    18601 tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct gagtattctg
    18661 gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg
    18721 aaatggtcga tgatatcatt gaatttgctg acatcggcga ccatatttat caacctgtaa
    18781 aaacctattc aagtgggatg ttcgttcgtc tagcttttgc tgtagcgatc aatgtgaatc
    18841 ctgatatttt gattgtggat gaagcattgg cagtaggaga tcttgaattt caactaaaat
    18901 gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc gtttctcatg
    18961 atattagttc gattcgccgt ttttgtgatc gttcctattg gttgaaaaat ggaaaagttg
    19021 tcgaatatgg tgatacgatg gaagtaacga cgaattacga aaactttttg aagaaaaaat
    19081 cagttaaaac agtagatcga aataaaaacc aagtagaacg cttcgcagct ccagatatcg
    19141 tagacgtgac gaaagccgaa ttgttggata aagataaaaa tccagtggat atgctggaac
    19201 aaggagaaaa actatacgtt aaagtaacct atgaagtaaa agacgataca atcaaaaatc
    19261 cagttttagg aattgctctg cgtacagtcg acaattttta tgtgtgcgga ctcaatactc
    19321 tactagatgg tatcaagatc ccttggaaaa aaggtgagaa tatcttttat ctggagtatg
    19381 aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg
    19441 caacggttcc tttggtatac aagacaaaat acatgaactt gtttgtcagc ggttcttata
    19501 tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat agcaatgaaa
    19561 tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc
    19621 aaaccaggaa gtaaagtttt agagtttggt cctggaaacg gccgattaac gaaacatttg
    19681 atcggagcaa aacaatgtga agtgagcatt gtcgagctag ataaggaatt gttcgatttt
    19741 gtcagcgagt ttgcccaaga tggattttat ggagatatcg aaagttttga atgggcaaac
    19801 tattatgctg gacaaacatt tgattacgtt ttgtttgccg atgttttaga acatttggta
    19861 gatcctggaa aaacgttgaa gaaagttaga gaatttttga atgaagaagg agaaattcta
    19921 attacattcc ctaaccttgc gcataattct gtcatgattg atttgttcaa taatcaattg
    19981 ccatgggctt cttatggttt gctagatgaa acccataatt ctttttatac tcatgatggc
    20041 ttccaaaaag tttttgaaaa agcaggatta tacatcaata ttgaagatta tttgtactta
    20101 gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc tgtacgttat
    20161 gatttcaaaa tgcgaccatt tggcgaagtc taccaatact tcttctcatt gatgaaacat
    20221 ccagtagcac aaagttccat tgcgcagccg caaaattcta attatgtgaa agtattagaa
    20281 gtaatccagc aaacgaaaca agatgaaata attcaacaaa taccattcaa taatttcaca
    20341 ggggaaaatg agacactaag tttccatgta tcagaattag cagaacgaat ggtgtttcgc
    20401 ttcgcgcagc aacctagctt tatcgaattc agcgcagaag ctgctggaga aaaactcaca
    20461 tttatcgatt caaatgctgt agtcaaaaca gtaaatgatt gttatttgtt tgatggtaaa
    20521 gatttaccag agttcttttt aacggatatt gcagggaaag aagtgactat ccattgtcac
    20581 tatcgtttta tcggagaatt gacaccaaca atgaaagaat tgctggaaac aatccgtcca
    20641 atggctgaag ttacaaagca gctttctgag aaaaatgatg aattagaaag agaaaatcat
    20701 tatcttattg agaataatac aagattagat cagacgttga aaacaaccat aaatcgctat
    20761 tgcacgttgt tggaaagtga tgaatggaca atcaaacata gattagggcg acgtaagaaa
    20821 gaaacatcga aaaaaatcca agaaaaagag ctttctctat gtattgacga aaaaatctgg
    20881 gatgcagaaa caaagatatt gaaaatcatc gggtggggaa tcgctaattc ggatcgccaa
    20941 ccattgtcct ataagttatc agccgaccaa tcaccatttt ttgaagcaat tcctgtttta
    21001 cgagaagaag tcaatcaagc aaaacagcta gctgaaggag caaaagcagg atttgaactg
    21061 cggatccttt gcgagaagga acgttcattc ttagttgaag cagtggctca aaatggtcaa
    21121 tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt aaacgaatcg
    21181 tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg gccactggcg
    21241 atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct acgataaagg
    21301 tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt aagattggat atcaatcatt
    21361 tatataatct tgatgttaag acccaaagcg gttttaaggt acgtttatca gggaaaatga
    21421 gaggaaaagc aatcttagac ttccaaacag caagacatca aaatggcata gctgtgaaat
    21481 taaacggtaa atatccttat gatgacggca ttgaatcttc atgggaaaga aaaaaaagat
    21541 tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagca atccgccggg
    21601 tcaaactgga attgaaacca ggatcgatcg actatgcaga gtggatcagt cgtcatgaga
    21661 aacctgatct gaaagagcaa aggaaagaag tacaaggatt tgcctaccgc ccattgattt
    21721 ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg
    21781 tacttgatca aacgtatgat cactgggaac tatgtatcag tgatgatgct tctactgatc
    21841 cagctatccg aaagtgtcta gaatcctatc aagcaaaaga tgatagaatt aaagtcgttt
    21901 tccgacaaga aaatggacac atcagtctgg caactaattc tgcattggaa atggcagaag
    21961 gagagtttat agcgctctta gataatgatg acgaattacc tccatttgcc ttatatgaag
    22021 tggcaaaagt gctaaacgta catccagaac tggatttgat ctacagtgac gaagataaaa
    22081 tcgatgcaga tgggaatcgc tttgacccgc acttcaaagc agactggtct cccgatacat
    22141 tgatgggaaa taattatatc tctcacttag gcgtatatcg gacaagcatt gttaaggaac
    22201 ttggcggttt ccgtaaagga tatgaaggct ctcaagatta tgatttggtt cttcgtgtga
    22261 cagaacaaat tcctgcagaa cacatttacc acattgaccg tgtcttgtat cattggcgga
    22321 cgatcccggg atcaactgca agcaatggag aagctaaaag ctacatctat gattctggag
    22381 tgaaagcatt gacagatgct ctatctagaa gaaatatcaa gggaagcgta catcctggca
    22441 gaatttctgg tttttatgaa atcacttatg atgttttgca agaagacttg gtcagtgtga
    22501 tcattccaac taagaatggc tatgaagatc taaaaacttg tgtggattcg atcatcgaaa
    22561 aaacgtctta tccaaattat gaaatcatta ttgcagataa tggaagtaca gatcctaaaa
    22621 tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgatttatc gtcgaattaa
    22681 ttgacattcc attcaattat tcacgtatca acaatctggc agcagaaaaa gcaaacggca
    22741 aatacttttt attcttgaat aacgatacag aagtgattga acctgactgg atgacagcga
    22801 tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaatta ttttatccgg
    22861 atgatacaac gcagcatgca ggtgtattat tagggatcgg tggcgttgca ggacatgcat
    22921 tgaataacta tgatcgaaca cattgtggtt actttggccg attggtcatt gacgtcaatt
    22981 acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat gctgtcaatg
    23041 gatttgatga aatattagaa gtagcattca acgatgttga cttgtgcttg aaagtttatg
    23101 aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc gaatctaaat
    23161 caagaggtta tgaagataca ccagaaaaac aaagacgttt tgctggggaa atcaaaaaaa
    23221 tgcaagataa gtggccttct tatattgctc atgacccatt ttataacgat aatctaacta
    23281 aagaaggaat cggcgatttt tcgttgagac ctgattgatt aaaagaactg tattttcatt
    23341 tttattaatg aaaatacagt tcttttaatt taattatatg atttgtcaaa ttaagctaga
    23401 ataaaatgct tcttgtacat agctcaaaaa tatttcgatt ggtgttctgt aattcaatga
    23461 ttttcttgga atatgattac gttgattgct gacactggaa ataaatgtct gattcacttt
    23521 tctaaaatcc attgatttcg gcagtccatt acgacgcaga atcccgttag aattctcgtt
    23581 taatgggcgt tgagaaggtg ttccagggtc cgcaaaatat atatcaatat catgttggtt
    23641 actaatggct ttccagttag aaaattcttt tccacagtca aacgtaatag atttaaagaa
    23701 gtttttaggg aagcgagaaa accattgatt aagggcagtt tcaatatcta atgccttacg
    23761 gccgttgggt ttaatcgtga taatgacttt agataatctt tcaactaaag taatgacggc
    23821 acttttatga tgaatgccaa cgatcgtatc accttcaagg tgaccaaact cagaattgaa
    23881 atcaggataa ttatcaggtc gatcatggat tgagcgctga tactgttgtt ttccccgttt
    23941 ttcctgatgg ccattgggtt ttcttttacc tttcatcggt agtgtgtcaa tatcaaatat
    24001 tcctttagaa aataaacgat aaagtgttct cataccgcat gaaacaggcc tttcttttcg
    24061 cccgataatg acgtcaggcg tccaaccaag agtgactttc tctttaatgt aatctacttc
    24121 atgagcaggt aattgaatga cttttctacc acattttttc ttattttctt tatactggtg
    24181 ccaataatca agagcagtct tgccttgctt gaacttattg actacattat aaatagtttg
    24241 tatagcacgt cccattcggt tagcgatttc aaccggctta ttatgttgaa gataatatga
    24301 ttctatcatt gtcagttcgt ctatggtaag atgtttgtag gtcatttatg gttacactcc
    24361 tttgttttct ttcgtcggaa atacaatttg agtgtaccat aaatgccttt ttatttttct
    24421 aacttaattt tacaattcgc gttatatgaa tattgtaact tgtgaataag gaattagcag
    24481 gagaatacta gttcagcata aaggaagtga tgggacttct gagacaactt tctgttatgc
    24541 agaaatacac atataaacag acaataaacg cttttccccg tttcttttaa tttcctttac
    24601 aatttggtta tacactaggt ttttaaactt tgctatggta agatgataga ggcattattt
    24661 tatggttagg agtgtatata tgaataaaaa tggggagtgg aatgcggcac gcagaatatt
    24721 cattattctt atggatgtac ttgtattaaa tgtatcgatc tatcttagtt ttttgctgaa
    24781 attccaggga gaagtaccag tgagaaactt agaaaccttt cagcattcag caatttttat
    24841 ttccatcatc tttattgcgt tgaatatttt gttaggagct tatgtattct ataaccgaat
    24901 gatcagtgat attgtcttca ttactgttat tggacaagta ctaatagcat tggggatcat
    24961 ggtagttact tttgctggcc gatggtttgc atttccgcgt ggcgtcattt tgatctcgtt
    25021 tatcctaggt acgatattac ttagtgttta ccggatagtc gtttacaaac tgtacttaag
    25081 agtcagtcgt gataagaaag tccttatcgt gggtttggag aaagatgtcg caccagctat
    25141 ccaaaatttt caatctaaga aaaacaataa acataaggtt gaagcagtgg caatcgataa
    25201 ttattacgaa aatacaaaaa aattgatcga tgatatggat atcgtctatt tggcttctaa
    25261 aatcccagaa gatgaaaaaa tcaacattta tcaattagtg actaaaaaag agaagaaatt
    25321 atttttaaat acgacttttg agaatctgac aatgataaat ccgaatatta tgaattttga
    25381 agatgaaagc atcattgaag cttctgggtt cagaatccaa ccagaatacg aactattcaa
    25441 gcgtctcttt gatatcatcg tttcgcttat tttactgatc ctcgcctcac cttttatgct
    25501 aatcacagcg attttggtaa aaacaacttc acctggacca atcatctata aacaagtacg
    25561 tattacgaaa aatcaaaagg aattttcaat ctataaattc cgttccatga gtgcgacagc
    25621 tgaagcaaaa tcaggaccag tcttggctaa aagtaacgat gcacgtgtga caccagtagg
    25681 taaatttatc cgtgctgtac gttttgacga attacctcaa atctttaatg tattgaaagg
    25741 cgatatgtcg attgtcggtc caaggccaga aagaccattt ttcgttgatc agttcaatga
    25801 agaaaatcct tattattact taagacacaa tgtacgtgcg ggaattaccg gatatgcaca
    25861 agtatacgga aaatatgtat cagattataa cagtaaacta cgttttgatt tgttgtatat
    25921 caaaaaatat tcattactat tagatttgaa gattttactt caaacaatca aaatcttatt
    25981 tgataagatg tcatctcaag gactagatga agatgaaaat attagttcat tagcagaaat
    26041 cgacttttca caagagaaaa tttatcgcta gattggaaaa attttgagca aagaggtagg
    26101 agagaacatt ggaaaataaa aatgtaagta tgctgctttc tttatatgat aaagaaaagc
    26161 cagagtattt aaaggaagca ttaactagta tttttgagca aacagtaatg ccaaaagaaa
    26221 ttgtattagt ttatgacggc cctataaatg aaaaacttca agtagttgtc acttatttcc
    26281 aacaaaaaga tcctcacttg tttacagttg taaaattaga aaaaaatcaa ggattgggga
    26341 ttgctttagc aattggatta gaacatacga aaaatcgctt agtagccaga atggatacag
    26401 atgatataat ggaaccccaa cgattagaaa aacaattaga gatcttccaa aaagatccag
    26461 aaatagcgat tgttgggagt aatattgagg aatttactgg cacgcttagc aatgttatcg
    26521 gaaaaagaat tgtacctgaa tataacaatg caattcgtgt attttctaaa agaagaaatc
    26581 cctttaatca tatgaccgtt atgtttgata aaaaagctat tttggaagtg ggaaattacc
    26641 aaccattaag tgggtttgaa gattattatc tttgggcaag attattaaag gcaggttata
    26701 aaggatataa tattcaagaa acattagtct atgcgcgaac tggcaaagat atgtatgcaa
    26761 gaagaggcgg aatgaaatat cttggttctg gtcttatggg tcgttatcgt atctggaaag
    26821 acggtttagg gtcagtaaaa gattttataa ttgtttcttg cggacatatt tttattagct
    26881 tactaccaaa tcaattaaga ggaaaatttt atcaaacgca attaagaaaa taggaggttt
    26941 ttctttgtat agcactgatc caaaaatgaa aaaaatacaa cagattacgt tgaaaatgtc
    27001 tcaagagatt gttgattttt gtctaaaaaa tcaacttctt tgttatttct gcggtggtgg
    27061 tgccatcggt actgtgagag aacaagggtt tattccatgg gatgatgatt tagatttctt
    27121 tatgcctaga gaggattatg aaaaatttta tcaactatgg gttaattcag aaaaaagtaa
    27181 aaaatatccc atacagaagg cttctaaaaa ttataatgat cataattctt ttacaactat
    27241 ccgcgatgca gatactacat ttattaaaac ttatcaaaga gatttagata tagtccatgg
    27301 aattactatt gatattttcc cgttagatgt tgcccctgtt tcgaaattta gccgtaaaat
    27361 tcaaaaacta tgggctttag tatatgcttt attttgttct caagtcgtcc ctgaaaaaca
    27421 tggtggaata atggctacag gtagtaagat actcttatct attttccgct cacctcgtac
    27481 gagatataag atatggtctt ttgctgaaaa acaaatgaca aaatataata atgaaccaac
    27541 gggctatgtg actgaacttt gtgtcggacc taaatacatg ggaaatgttt atcctgcaaa
    27601 agactttgaa tctgctgtgt gggtgccatt cgaagataca gagatgccag taccagttgg
    27661 ttatgataat tatttaagtc aagtttttgg tgactatatg cagcttccgc ctgaaaaaga
    27721 tcaggtttct catcatgaag cagtttatat tgatccagaa catccataca caatgtacaa
    27781 aggaaaatat tatttaacta aaggagcaga aaaaaaatga ttacagctat tattattgct
    27841 ggtggtgtgg gtaaacgaat gggacaagat atcccaaaac agttcattac aattgaagga
    27901 aaacctatta ttatctatac tttagaatca tttcaacacc atcctcaagt tgaccggatt
    27961 ttagtcgttt gcaaatctgg ttgggaagaa acgatgtggg cgtatatcaa agaattcaat
    28021 attagcaaag tcaaatgggt cattacaggt ggatcaaagg gacaagagtc gatcaataat
    28081 ggtgttcagt ttttgaaaga ctattctgaa gatgatgata cgatcatcat ccatgatgga
    28141 atccgtccac tagtagacga actggtattg tcagatgtaa tcgttaaatg taaagaattt
    28201 ggtaacggtg taacctctct accttacaac gaacaaattt ttatcaagaa aacagaagaa
    28261 acgacggaac agtatgtcga tcgtaacaca ttgaggcgtg tctcaactcc acaagcttat
    28321 caatacggaa aactacgggc agcatatgac cgggcagtga aagaagacat cggtatgact
    28381 gattcttctt atacgaatac catgatggta gatttgggag aaactttata ttttgctgct
    28441 ggttctgata aaaatatcaa attgacaaca acagatgatc tagaattgtt caaagcatat
    28501 ttaaaaatga aagactaggt gaaagatgat gacgttgatt aaacaaccaa tgtaccaaga
    28561 agatttggca aaagtaatca atcatacacc aaaaataaaa cagctacatg gaaaatccgt
    28621 tctgatgatt ggtgcttctg gaatgatcgg ctcttttttg attgatacgt tgatggctgc
    28681 taatgaacaa ctaggcatca aaatcaaagt ttatgcaatg ggcagaaacc gtcagaagct
    28741 tgaaaaaagg tttgccagtt atcttgaatt agatactttc gaaattgttg aaggtgacgt
    28801 cacagaaccc ttaccaaaag aaattcaagc tgactatttg atacacggtg caagtaacac
    28861 acatcctaag gcttacgcaa ctgatccaat cggtacgatc atgactaatc ttgcaggtac
    28921 agagcaagta ttgaaacatg cagtagcaac ccaaccggtt agagtattat ttttatcaac
    28981 agtagaaatc tatggcgaaa atcgaggcga tatagaaaaa ttcacagaag attactgtgg
    29041 atacatcgat tgtaatacac tgagagcagg ctaccctgaa ggaaaaagag tcagtgaatc
    29101 attatgccaa gcctacatcg caaaacacga cctcgacatc gtgattccaa ggctttgccg
    29161 tacgtttggt ccgacgatgc ttttatctga tacgaaagca tcctcacaat ttattttaaa
    29221 tgcagtcgaa caaaaagata ttgttttaaa aagtgaagga aaccaatatt tttcgtatgt
    29281 atatgttgga gatgctgttt ctgctattct tcatttactg cttgaaggag aaaaaggaga
    29341 ggcgtacaac gtttcttcag aaaactttga tttacgacta aaagaattag cccaacagct
    29401 agctgatatc agcggcaaac aagtaatttt tgaattgccg gatgagattg aacaaaaagg
    29461 tttctcaaaa gctagcacag cagtacttga aaataaaaaa attaaagaga caggttggaa
    29521 accaattttt cctttgaacg aatcattggt tcatacagta gaattagtta aaggtgagaa
    29581 atataatgtg tgagattagt attattgttc ctgtgtataa agttgaaaaa tatttaagaa
    29641 aatgtgtaga ttcgatttta gcccaaacat ttactgattt tgaagttata ttagtagatg
    29701 atggatctcc tgacaatagc gggaaaatct gtgatgagta tgctgaaaaa gataatcgag
    29761 tacgtgtgat ccataaagaa aacggcggat taagcagtgc tagaaatgcg gggattgatg
    29821 ttgcgagagg caaatactta ggctttgttg acagtgatga ttatattgat gaagatatgt
    29881 atgaaatttt gtatgagaat ttgaaaattc atgatgccga cattagctct gttgaattga
    29941 ttccttttta tggtgataga tataagaaag ctaataaaga aaagaaagta attatattaa
    30001 ataaaaagga agcaataaaa tctgttttag aaggaacgca attttatgca tacgcatgga
    30061 ataaacttta tcgtaaggaa ctttttaaag ataatcgtta cttggatgga aaaacatt
//
LOCUS       DAYYGY010000028        30024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000028 DAYYGY010000000
VERSION     DAYYGY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.028"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2424)
                     /locus_tag="ACHA52_001236"
     CDS             complement(61..2424)
                     /locus_tag="ACHA52_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8939973.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2589..3093
                     /locus_tag="ACHA52_001237"
                     /pseudo
     CDS             2589..3093
                     /locus_tag="ACHA52_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHA52_001238"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHA52_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8939974.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHA52_001239"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHA52_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8939975.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="ACHA52_001240"
     CDS             complement(5894..6205)
                     /locus_tag="ACHA52_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8939976.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="ACHA52_001241"
     CDS             complement(6202..6495)
                     /locus_tag="ACHA52_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8939977.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHA52_001242"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHA52_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8939978.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHA52_001243"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHA52_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8939979.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="ACHA52_001244"
     CDS             complement(8333..12685)
                     /locus_tag="ACHA52_001244"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8939980.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="ACHA52_001245"
     CDS             complement(12897..14606)
                     /locus_tag="ACHA52_001245"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8939981.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHA52_001246"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHA52_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8939982.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="ACHA52_001247"
     CDS             complement(16103..16903)
                     /locus_tag="ACHA52_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8939983.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="ACHA52_001248"
     CDS             complement(16900..17712)
                     /locus_tag="ACHA52_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8939984.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHA52_001249"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHA52_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8939985.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHA52_001250"
     CDS             complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHA52_001250"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8939986.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHA52_001251"
     CDS             complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHA52_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8939987.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHA52_001252"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHA52_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8939988.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="ACHA52_001253"
     CDS             21446..21925
                     /locus_tag="ACHA52_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8939989.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="ACHA52_001254"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="ACHA52_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /gene="phnX"
                     /locus_tag="ACHA52_001255"
     CDS             23226..24011
                     /gene="phnX"
                     /locus_tag="ACHA52_001255"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8939990.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHA52_001256"
     CDS             complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHA52_001256"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8939991.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHA52_001257"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHA52_001257"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8939992.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHA52_001258"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHA52_001258"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8939993.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHA52_001259"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHA52_001259"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8939994.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8012 a         6455 c         5344 g        10213 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa ttta
//
LOCUS       DAYYGY010000029        28637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000029 DAYYGY010000000
VERSION     DAYYGY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.029"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACHA52_001260"
     CDS             85..624
                     /locus_tag="ACHA52_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8939995.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="ACHA52_001261"
     CDS             1644..2813
                     /locus_tag="ACHA52_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8939996.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="ACHA52_001262"
     CDS             3286..3840
                     /locus_tag="ACHA52_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8939997.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="ACHA52_001263"
     CDS             complement(4013..4486)
                     /locus_tag="ACHA52_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8939998.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5445
                     /locus_tag="ACHA52_001264"
                     /pseudo
     CDS             4498..5445
                     /locus_tag="ACHA52_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5716..6151
                     /locus_tag="ACHA52_001265"
                     /pseudo
     CDS             5716..6151
                     /locus_tag="ACHA52_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            6167..7000
                     /locus_tag="ACHA52_001266"
     CDS             6167..7000
                     /locus_tag="ACHA52_001266"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8939999.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7245..8270
                     /locus_tag="ACHA52_001267"
     CDS             7245..8270
                     /locus_tag="ACHA52_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8940000.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8408..8533
                     /locus_tag="ACHA52_001268"
     CDS             8408..8533
                     /locus_tag="ACHA52_001268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940001.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            8558..9673
                     /locus_tag="ACHA52_001269"
                     /pseudo
     CDS             8558..9673
                     /locus_tag="ACHA52_001269"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            9756..10652
                     /locus_tag="ACHA52_001270"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="ACHA52_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            10668..11492
                     /locus_tag="ACHA52_001271"
     CDS             10668..11492
                     /locus_tag="ACHA52_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940002.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="ACHA52_001272"
     CDS             11524..12834
                     /locus_tag="ACHA52_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8940003.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="ACHA52_001273"
     CDS             12982..14241
                     /locus_tag="ACHA52_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8940004.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="ACHA52_001274"
     CDS             14251..15123
                     /locus_tag="ACHA52_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940005.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="ACHA52_001275"
     CDS             15135..15965
                     /locus_tag="ACHA52_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940006.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="ACHA52_001276"
     CDS             16005..18188
                     /locus_tag="ACHA52_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8940007.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="ACHA52_001277"
     CDS             18249..19469
                     /locus_tag="ACHA52_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8940008.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="ACHA52_001278"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="ACHA52_001278"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8940009.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="ACHA52_001279"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="ACHA52_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="ACHA52_001280"
     CDS             22431..22640
                     /locus_tag="ACHA52_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8940010.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="ACHA52_001281"
     CDS             22633..22782
                     /locus_tag="ACHA52_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8940011.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="ACHA52_001282"
     CDS             22936..23601
                     /locus_tag="ACHA52_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8940012.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACHA52_001283"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACHA52_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8940013.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="ACHA52_001284"
     CDS             25304..25537
                     /locus_tag="ACHA52_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8940014.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="ACHA52_001285"
     CDS             25534..25941
                     /locus_tag="ACHA52_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8940015.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="ACHA52_001286"
     CDS             complement(27078..27596)
                     /locus_tag="ACHA52_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8940016.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27963..28211
                     /locus_tag="ACHA52_001287"
     CDS             27963..28211
                     /locus_tag="ACHA52_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940017.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9843 a         4314 c         5246 g         9234 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcggtact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagc
//
LOCUS       DAYYGY010000030        28555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000030 DAYYGY010000000
VERSION     DAYYGY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.030"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHA52_001288"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHA52_001288"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8940018.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHA52_001289"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHA52_001289"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8940019.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHA52_001290"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHA52_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8940020.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHA52_001291"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHA52_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8940021.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHA52_001292"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHA52_001292"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8940022.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHA52_001293"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHA52_001293"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8940023.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACHA52_001294"
     CDS             complement(8557..9027)
                     /locus_tag="ACHA52_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8940024.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACHA52_001295"
     CDS             complement(9073..9498)
                     /locus_tag="ACHA52_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8940025.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACHA52_001296"
     CDS             complement(9565..9834)
                     /locus_tag="ACHA52_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8940026.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9866..10825)
                     /locus_tag="ACHA52_001297"
     CDS             complement(9866..10825)
                     /locus_tag="ACHA52_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8940027.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(10907..12505)
                     /locus_tag="ACHA52_001298"
     CDS             complement(10907..12505)
                     /locus_tag="ACHA52_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8940028.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIRRL"
     gene            complement(12520..16041)
                     /gene="mfd"
                     /locus_tag="ACHA52_001299"
     CDS             complement(12520..16041)
                     /gene="mfd"
                     /locus_tag="ACHA52_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8940029.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFART
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(16058..16618)
                     /gene="pth"
                     /locus_tag="ACHA52_001300"
     CDS             complement(16058..16618)
                     /gene="pth"
                     /locus_tag="ACHA52_001300"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8940030.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            16971..17945
                     /locus_tag="ACHA52_001301"
     CDS             16971..17945
                     /locus_tag="ACHA52_001301"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8940031.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            18115..19515
                     /locus_tag="ACHA52_001302"
     CDS             18115..19515
                     /locus_tag="ACHA52_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8940032.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(19593..20315)
                     /locus_tag="ACHA52_001303"
     CDS             complement(19593..20315)
                     /locus_tag="ACHA52_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8940033.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(20394..20900)
                     /locus_tag="ACHA52_001304"
     CDS             complement(20394..20900)
                     /locus_tag="ACHA52_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8940034.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            21023..22822
                     /locus_tag="ACHA52_001305"
     CDS             21023..22822
                     /locus_tag="ACHA52_001305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8940035.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(22890..24464)
                     /locus_tag="ACHA52_001306"
     CDS             complement(22890..24464)
                     /locus_tag="ACHA52_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8940036.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(24719..25228)
                     /gene="mreD"
                     /locus_tag="ACHA52_001307"
     CDS             complement(24719..25228)
                     /gene="mreD"
                     /locus_tag="ACHA52_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8940037.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(25232..26086)
                     /gene="mreC"
                     /locus_tag="ACHA52_001308"
     CDS             complement(25232..26086)
                     /gene="mreC"
                     /locus_tag="ACHA52_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8940038.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(26241..26879)
                     /locus_tag="ACHA52_001309"
     CDS             complement(26241..26879)
                     /locus_tag="ACHA52_001309"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8940039.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(26880..27530)
                     /gene="rpe"
                     /locus_tag="ACHA52_001310"
     CDS             complement(26880..27530)
                     /gene="rpe"
                     /locus_tag="ACHA52_001310"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8940040.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(27543..28442)
                     /gene="rsgA"
                     /locus_tag="ACHA52_001311"
     CDS             complement(27543..28442)
                     /gene="rsgA"
                     /locus_tag="ACHA52_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8940041.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
BASE COUNT         7871 a         6128 c         5179 g         9377 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttctcctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg ggatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact tgggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaactctt aattgtcaaa ttaagttaga cagatcatca atacttacgt
     9901 aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga atattatttc
     9961 gtttagatgc gacagattga ataaaaaatt catctactga attgaaatcc atagatttca
    10021 ataaaccatc tctacgtaac aatccgttag aattctcgtt taggcctctt tgagacggcg
    10081 ttcctggatc agcgaaataa atggcaatat cattgacatt actgatctgt ttccaatttg
    10141 aaaattcctt tccacaatca aaagtgatgg atttgaatag gtttttcggt acagattcaa
    10201 accattgatt taattttttt tcaatatcaa tcgcttgtct accacacggt ttcaatgtga
    10261 tgataacttt tgataatcgt tcaactaagg taattacagc acttttatgt ttcagaccta
    10321 cgatagtgtc accttcaagg tgaccaaact catttgagaa ttggctataa tccttttcac
    10381 gttcatgaat agagcggcgg aaagtttgtt ttccacgtct ttcttgatgt ccattcggtt
    10441 tacgcttgcc tttcatcggt aagtcagaag aatcaaatag cccctttttg aacatacgat
    10501 aaatggtacg agcagaacaa ctaataggaa acgctgcacg accaacaatc acatcgggtg
    10561 tccatccttg aacaaccttt ctttgaatat attctgattg ttcctcgggt aaaacaagcg
    10621 gacgtctacc acagtttgat ttgtttttct tatactgttg atagtactct aaggctgatt
    10681 ttccttgctt gaagaacagg tatactttat ggatcgtttg tcttgaacga ttcaagcaat
    10741 gggcagtttt agcaacagat tgattcattt tgaaataaga ctctattatt acaagttcat
    10801 ccgttgtaag atgggtatag gtcatttgta ctcactcctt ataattcttt ggtcggaact
    10861 attttgagtg tagcacaaat gactttttta gttgtctagc ttaattttac aatcggcgaa
    10921 tttttttatt taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg
    10981 taaacagacg aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta
    11041 tcacgctgac aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata
    11101 caccataata cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc
    11161 caaaatgccg ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc
    11221 aaagcgtcac acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac
    11281 cggtcagaaa agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag
    11341 aagggcgaaa cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga
    11401 aagcaatgga aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg
    11461 cataattgat ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg
    11521 tcgtcaatct aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca
    11581 aatgacgagt caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta
    11641 attgtactaa aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg
    11701 acagtccgta aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga
    11761 taagcaatcc actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg
    11821 gctttgacgg ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct
    11881 tttgttcata gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca
    11941 ttgctgccgt ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac
    12001 agacaatcac aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca
    12061 tcaagaatct tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg
    12121 taaaggaaac tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc
    12181 agctgctaaa tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat
    12241 aaagattttg caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg
    12301 atatgaactg cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa
    12361 cttgctgata aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa
    12421 gggcacttaa tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga
    12481 cgcgccgcat ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg
    12541 ctcacgcaac gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg
    12601 gttggggatc gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag
    12661 ttttgtcgct gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt
    12721 acttagagta aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc
    12781 gccatccatt ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc
    12841 aaaacggtcg agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg
    12901 aattcgttta tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata
    12961 cgcatcgaca ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg
    13021 tttacgagaa acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat
    13081 aaatccatgc tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat
    13141 cgcaatcttg aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc
    13201 actgacttcg ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct
    13261 gcttcttcct acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt
    13321 ctcaacgaac aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt
    13381 gacaagaaca tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc
    13441 tgtcatttgc ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc
    13501 tactttttgt tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc
    13561 catttctcga tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg
    13621 gatcggataa cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat
    13681 cgacatatgc aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc
    13741 acgtaactgt ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat
    13801 cagtagaccc aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac
    13861 aatatcaact tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct
    13921 gaaacgactt aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc
    13981 atagtgctgc tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt
    14041 tatcgcttta aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac
    14101 gagtagtcga tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct
    14161 caactggtca tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc
    14221 atcaggtcca aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc
    14281 gtcagcgata tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc
    14341 gcctagctta ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt
    14401 cagttgagta acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc
    14461 ttggtgaacg ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt
    14521 ggcatggaca acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt
    14581 tgttactgtt tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt
    14641 gatagctact attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa
    14701 ttgtacctgt cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc
    14761 tcgaagcatt tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt
    14821 ctgtttttgc ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa
    14881 ctgttgcatc gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc
    14941 tttttggaag agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat
    15001 atctgctgaa aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca
    15061 ttctgccgct tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac
    15121 gactagtaaa ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag
    15181 atctgagtag aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg
    15241 accgaagtaa tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt
    15301 ttctagcatt ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg
    15361 ttcactcgtt ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc
    15421 ttcaaaatac cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt
    15481 tgtcgttaac gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc
    15541 gaccatctct tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc
    15601 tatctcatca ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag
    15661 tcgtttgtgc aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc
    15721 gttaagcgtt gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga
    15781 aagcacttca tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc
    15841 ttctgctaac tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt
    15901 tttcttaaac gttcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag
    15961 ttgtctaggc attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc
    16021 gactaattga ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat
    16081 ccacgaacga atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt
    16141 cttctatcaa aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt
    16201 ttttcccttc tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat
    16261 gggaaataat acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga
    16321 tttttcccac tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc
    16381 caaaataagt cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt
    16441 tgactaaaag gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat
    16501 tttttttgaa agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat
    16561 tgtgttttgt atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt
    16621 ttgttttcca cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca
    16681 gtccatcgag cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc
    16741 gtttcggcta aaagagaatc gttaataaat gataaataaa ttaaaaattt cttcaaagtt
    16801 taatgaaata attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata
    16861 atacaacctt gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa
    16921 tgttagcata agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag
    16981 ctgcagaaaa aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta
    17041 gctatgcctt tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca
    17101 atactgctaa aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac
    17161 ctaaaaaaat ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta
    17221 cagctggcgc accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa
    17281 aaatcaaccg cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag
    17341 ttgccgctaa cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta
    17401 aagaacgcgt aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg
    17461 cggaattagt tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag
    17521 attcagaatt cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg
    17581 taaaaaacaa cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg
    17641 acgctgctta cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat
    17701 tagcacgtat cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat
    17761 tcatgaatgg cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc
    17821 gtcaaggtat ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg
    17881 ctgcttctgc taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg
    17941 aataatcttt gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc
    18001 acccttatgg tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa
    18061 ttatgctatc atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca
    18121 agatcttcgc atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc
    18181 ttagtgctga tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg
    18241 aatacagaaa taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa
    18301 ttaaaagaag cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag
    18361 gaagtaccaa aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc
    18421 ctaaatgaac aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa
    18481 gaaatagatc ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca
    18541 actaagctga ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa
    18601 gcaggtgact ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct
    18661 gcggtcaaac aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa
    18721 ttcaaagaaa aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc
    18781 atgaatgcta tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc
    18841 ccatcaagtg atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca
    18901 gaacaagtac gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac
    18961 acaaaattta aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg
    19021 acgatccaaa cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat
    19081 tttactcgtt cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat
    19141 acgtatatcg aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg
    19201 gcccttgaaa cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc
    19261 tatgtttgga ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct
    19321 tacgagagtc cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat
    19381 tcagactggc aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc
    19441 atcaacaccc caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct
    19501 gtattggtct tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg
    19561 tgctttcttt ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct
    19621 gaaacatcat catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc
    19681 tcatttaaat caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac
    19741 tctttttcca actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat
    19801 acttcaggag aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc
    19861 tcttcatcaa cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag
    19921 taactgactg aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca
    19981 tttgtcgctg tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc
    20041 ggaccatatc cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc
    20101 tttttaatcg cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca
    20161 acaagccgca aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa
    20221 atttcacgag aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt
    20281 ccttggatat tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga
    20341 gaatacactt ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa
    20401 cgcttcttga atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa
    20461 tccaacatga catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg
    20521 tccaaacctg cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt
    20581 tttagcttgg ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa
    20641 tgtgcgccct tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc
    20701 accatacttc caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta
    20761 ggcagccagc tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg
    20821 taaaataaga ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt
    20881 tgtttttttg aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa
    20941 acaacaatca ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt
    21001 acaatatgaa gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt
    21061 ttccggaggc agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat
    21121 gaacgagtat tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt
    21181 aaacgctatt cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta
    21241 tattactgca ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa
    21301 gctttacgca atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc
    21361 agaaatatct gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc
    21421 ttttcgttta aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa
    21481 cattataaat acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat
    21541 tgtagccagc atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg
    21601 acaagccttc aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc
    21661 agcatgggaa gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt
    21721 agatggtttt agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc
    21781 attagagtac aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa
    21841 aaacaaacaa ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa
    21901 aatggaatgg caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt
    21961 tagttttgac gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat
    22021 ggctaaattt gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg
    22081 gaaacgtcct ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat
    22141 gacctacagt aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt
    22201 ccatagcagc acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc
    22261 agaaacagca agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa
    22321 aacaaaagaa gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt
    22381 catgaatatc cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg
    22441 attagtctca gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca
    22501 aggagcttta gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga
    22561 tgacgtaccg ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta
    22621 tgcttacgcc aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga
    22681 tactgcctca atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca
    22741 accagatttt tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga
    22801 attgacagaa gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga
    22861 atgggctaac tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac
    22921 cattgaactg aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct
    22981 ccgcctaaag caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct
    23041 ccagcttttg cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa
    23101 ccaccgatgt cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca
    23161 aagccacttg gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct
    23221 gctacgattg ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt
    23281 ggttgttcag gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct
    23341 gtatcaacac tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt
    23401 gttgaagatt caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt
    23461 gattcttctg ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca
    23521 ggcgctgttg attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat
    23581 tgtgtagctg atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt
    23641 gctgagcttt gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg
    23701 cgtgcttctt tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt
    23761 tgttgttcac gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca
    23821 gctttcttgt cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa
    23881 tcagcttgtt tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct
    23941 aattctttta gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt
    24001 ttttgttgtt ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt
    24061 ccaatagcat ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg
    24121 ttgcttactt gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt
    24181 tcttgtaaat ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt
    24241 tttgctaata ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg
    24301 atttgtgatt gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt
    24361 tgtatctgag aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag
    24421 gtcattgaac ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac
    24481 tggcttaatt taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt
    24541 accatagcag tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt
    24601 taaaaatgac aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag
    24661 catttcttaa gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt
    24721 attcatcact gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca
    24781 acagcagtgt aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt
    24841 taaaaatcaa ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac
    24901 tgaaaaattc agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata
    24961 caattaaagg gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga
    25021 tagccccgag aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac
    25081 ataataatgc caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc
    25141 ctagcattct tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat
    25201 agtacttcat tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg
    25261 tcacaactga cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc
    25321 cataactatc tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc
    25381 ctccaagacc ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt
    25441 gggtcaccac taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc
    25501 cattagccga agatacccgt actggaaaat gattggagct ttcattagag gatgacagta
    25561 attcgatttt agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg
    25621 acatgactgc catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt
    25681 cctgccaact gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca
    25741 gcgtctgatt caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt
    25801 tttgctgtga aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat
    25861 aagttgaaaa aagattatgg acagaagcta ctccatttcg tacccaccgt actggggcag
    25921 agattgtttt atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct
    25981 ttccttcatt cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa
    26041 taaccaaggt aataataata tttttattag gattgaactt cttcacaaag acacctcgaa
    26101 ttaattagac tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc
    26161 tcctcttaag ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa
    26221 tttttttatt tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa
    26281 aaagaaaatt ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct
    26341 acttctttat tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc
    26401 aagcagcaat aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc
    26461 aaataataag acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg
    26521 aagcgaggtt ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta
    26581 gctccaatca aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac
    26641 tgtgtatctg tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat
    26701 acttccttct gctcttcgtt cgacaatgaa tcaaaatcac ccactgccag atctactggg
    26761 tagcctgctt gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg
    26821 gacagcagag actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt
    26881 tagtctaatg cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata
    26941 tatgatcctg caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga
    27001 acgattccgc catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg
    27061 agttccgtaa ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg
    27121 acagtcatga ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt
    27181 gttcctggat tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc
    27241 cgatgaatat gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca
    27301 aatgctggaa tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta
    27361 gtaacaggac ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg
    27421 ccatccatta catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct
    27481 ctttgtaaat tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc
    27541 tcctattttc gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt
    27601 aagaattgca aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt
    27661 tttacttcgc agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa
    27721 gcttctacaa attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca
    27781 atcgagctaa atcctggtgt atctgctact aacccgccat agataggaat cagttccaca
    27841 tgacgtgttg tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc
    27901 agttccggtg atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag
    27961 accgatagcc gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt
    28021 acaatcaccg gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg
    28081 tcgtctgaca agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag
    28141 gtaactagaa agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca
    28201 atgattccaa gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag
    28261 atttccagaa gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca
    28321 agcggcgtga ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg
    28381 tttgcgtata catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc
    28441 acctccgttt ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac
    28501 ttctcaaagt atatggcttg aaaattgatc aaaagagata gaaacacaaa aaaga
//
LOCUS       DAYYGY010000031        28067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000031 DAYYGY010000000
VERSION     DAYYGY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.031"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..695
                     /locus_tag="ACHA52_001312"
     CDS             48..695
                     /locus_tag="ACHA52_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8940042.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            779..1723
                     /locus_tag="ACHA52_001313"
     CDS             779..1723
                     /locus_tag="ACHA52_001313"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8940043.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1858..3309
                     /locus_tag="ACHA52_001314"
     CDS             1858..3309
                     /locus_tag="ACHA52_001314"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8940044.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3330..4166
                     /locus_tag="ACHA52_001315"
     CDS             3330..4166
                     /locus_tag="ACHA52_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8940045.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4166..4825
                     /locus_tag="ACHA52_001316"
     CDS             4166..4825
                     /locus_tag="ACHA52_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8940046.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4951..5640
                     /locus_tag="ACHA52_001317"
     CDS             4951..5640
                     /locus_tag="ACHA52_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940047.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5646..6602
                     /locus_tag="ACHA52_001318"
     CDS             5646..6602
                     /locus_tag="ACHA52_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8940048.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6646..7158
                     /locus_tag="ACHA52_001319"
     CDS             6646..7158
                     /locus_tag="ACHA52_001319"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8940049.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7414..7962)
                     /locus_tag="ACHA52_001320"
     CDS             complement(7414..7962)
                     /locus_tag="ACHA52_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8940050.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8051..9232)
                     /locus_tag="ACHA52_001321"
     CDS             complement(8051..9232)
                     /locus_tag="ACHA52_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8940051.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9468..10976)
                     /locus_tag="ACHA52_001322"
     CDS             complement(9468..10976)
                     /locus_tag="ACHA52_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8940052.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11135..11326
                     /locus_tag="ACHA52_001323"
     CDS             11135..11326
                     /locus_tag="ACHA52_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940053.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11529..12446
                     /locus_tag="ACHA52_001324"
     CDS             11529..12446
                     /locus_tag="ACHA52_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8940054.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12439..13512
                     /locus_tag="ACHA52_001325"
     CDS             12439..13512
                     /locus_tag="ACHA52_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940055.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13660..14178
                     /locus_tag="ACHA52_001326"
     CDS             13660..14178
                     /locus_tag="ACHA52_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940056.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14159..14551
                     /locus_tag="ACHA52_001327"
     CDS             14159..14551
                     /locus_tag="ACHA52_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8940057.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14627..15562
                     /locus_tag="ACHA52_001328"
     CDS             14627..15562
                     /locus_tag="ACHA52_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8940058.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15695..16475
                     /locus_tag="ACHA52_001329"
                     /pseudo
     CDS             15695..16475
                     /locus_tag="ACHA52_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            16468..16785
                     /locus_tag="ACHA52_001330"
     CDS             16468..16785
                     /locus_tag="ACHA52_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8940059.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17029..18639
                     /locus_tag="ACHA52_001331"
     CDS             17029..18639
                     /locus_tag="ACHA52_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8940060.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18796..19644
                     /locus_tag="ACHA52_001332"
     CDS             18796..19644
                     /locus_tag="ACHA52_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8940061.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19875..20354
                     /locus_tag="ACHA52_001333"
     CDS             19875..20354
                     /locus_tag="ACHA52_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8940062.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20387..22615
                     /locus_tag="ACHA52_001334"
     CDS             20387..22615
                     /locus_tag="ACHA52_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8940063.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22744..24387
                     /locus_tag="ACHA52_001335"
     CDS             22744..24387
                     /locus_tag="ACHA52_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8940064.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24460..25005)
                     /locus_tag="ACHA52_001336"
     CDS             complement(24460..25005)
                     /locus_tag="ACHA52_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8940065.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25028..25513)
                     /locus_tag="ACHA52_001337"
     CDS             complement(25028..25513)
                     /locus_tag="ACHA52_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8940066.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25503..26315)
                     /locus_tag="ACHA52_001338"
     CDS             complement(25503..26315)
                     /locus_tag="ACHA52_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8940067.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26454..27566)
                     /locus_tag="ACHA52_001339"
                     /pseudo
     CDS             complement(26454..27566)
                     /locus_tag="ACHA52_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(27587..>28067)
                     /locus_tag="ACHA52_001340"
     CDS             complement(27587..>28067)
                     /locus_tag="ACHA52_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8940068.1"
                     /translation="AKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVL
                     ETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRK
                     TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         9176 a         5112 c         5610 g         8169 t
ORIGIN      
        1 ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg
       61 ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc
      121 aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact
      181 cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta
      241 ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc
      301 cagatactgc aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga
      361 tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa
      421 tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag
      481 aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg
      541 acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt
      601 attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa
      661 caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg
      721 atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt
      781 gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg
      841 tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt
      901 tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac gctattttga
      961 tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt cttattatct
     1021 agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc
     1081 cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg
     1141 gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact
     1201 agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa
     1261 gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga
     1321 tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt
     1381 gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat
     1441 tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt
     1501 gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga
     1561 acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata aagaagggcc
     1621 ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac
     1681 gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca
     1741 tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta
     1801 ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg
     1861 aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta
     1921 gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg
     1981 cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga aaaagggcca
     2041 ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct
     2101 acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa ttatgtgcgg
     2161 caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga gtttggtttc
     2221 aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa ccttgaatgt
     2281 acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat cgttccttta
     2341 gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg cggattgatt
     2401 gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag aggatggcaa
     2461 ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc tttattttat
     2521 gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga gtggcaaaca
     2581 catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat ccactcacag
     2641 attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg
     2701 catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac gtcttattat
     2761 cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg catccagcga
     2821 gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata tggtcagaag
     2881 ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc aattgtcatg
     2941 gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt taccgcccct
     3001 aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca catcagtgat
     3061 tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag cttgaagatc
     3121 acaccttata cacagttggc taatctgact ggacaaccag caatcagctt gcctactcat
     3181 ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg aagagaagat
     3241 ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt accaaatcca
     3301 tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt gctctaataa
     3361 ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag gaagataaca
     3421 gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc acactgtttc
     3481 ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat ttggcaaaag
     3541 aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg gatggacaaa
     3601 tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt atcacaaaag
     3661 aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac agtatgatcg
     3721 tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg aaaaaagcag
     3781 ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct aaattagtca
     3841 catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt cgaacagatg
     3901 ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca gatatccctg
     3961 taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta ttgaaagaag
     4021 gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa gaagatggga
     4081 cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa
     4141 cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt ccctcatgtg
     4201 gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc
     4261 gtttctttta ggaaatctgg tagggttgct tttgacagtt ttaggtcttt tggattggct
     4321 gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc ccgcactagt
     4381 cctgctgttt ctgttatatt ttgggttacc ttatcaatta agtgccttaa cggcttcagt
     4441 catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag gatcactagc
     4501 aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt tctttggggt
     4561 attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt
     4621 agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc cagaaatatt
     4681 tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct atattttagt
     4741 tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa
     4801 acgttctatg catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt
     4861 tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca tgtaaaatgt
     4921 aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa
     4981 tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg
     5041 cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga tggaagccga
     5101 gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac agccaaactg
     5161 tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga agaagtaaaa
     5221 actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag
     5281 atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga gcttgctgta
     5341 ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt aagaaaagca
     5401 aagcaaatgg agcgaggcgt aaaatatttt ccaacagatc aattgtacaa agaattgaaa
     5461 agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg gtttttagag
     5521 gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct tcagcatgat
     5581 tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc attggagtga
     5641 aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca ttaaaagaag
     5701 cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca gtacgtgatt
     5761 ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga
     5821 tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag
     5881 tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag
     5941 tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag
     6001 aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct
     6061 ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc
     6121 atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc
     6181 acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata
     6241 aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata
     6301 tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt
     6361 ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga
     6421 ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg
     6481 gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc
     6541 atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat
     6601 gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa
     6661 acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt
     6721 gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg
     6781 aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca
     6841 gctgcattag tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa
     6901 tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa
     6961 aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa
     7021 gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct
     7081 ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat
     7141 caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact
     7201 gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac
     7261 aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa
     7321 atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgacct
     7381 ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt tttttatttt
     7441 atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt
     7501 caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc
     7561 agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa
     7621 gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa
     7681 aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata
     7741 tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa
     7801 tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg
     7861 attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt
     7921 tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat
     7981 gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct
     8041 tttttgtata ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg
     8101 ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc
     8161 ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga
     8221 cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca
     8281 tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt
     8341 ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt
     8401 ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa
     8461 aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc
     8521 ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt
     8581 cttctaatac atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa
     8641 caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca
     8701 tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg
     8761 gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc
     8821 cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt
     8881 tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata
     8941 caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa
     9001 aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata
     9061 cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta
     9121 aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa
     9181 aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc aaataatcac
     9241 tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta atctttagtg
     9301 aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa ataaagcgaa
     9361 caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat
     9421 cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta ttggtaatat
     9481 agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt
     9541 tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc aagttcagct
     9601 agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga taaagcgatc
     9661 aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct
     9721 tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat atgggctaaa
     9781 tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact tgtacgcccg
     9841 gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc ttttgtagga
     9901 ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt tcggtcttca
     9961 ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc ttcgtcgatc
    10021 aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc ttgtttagag
    10081 gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac acccatgtca
    10141 gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg tttgattaca
    10201 ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc atcaaagtgg
    10261 aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata agcaatgttc
    10321 agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga gtaaccagct
    10381 gcatgtcctt tttggctttc atgatacagt tggttcaagg aagtagcgag ctttccactt
    10441 cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata ctcatttttt
    10501 ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc gatttctgag
    10561 tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt cgtggcaggc
    10621 tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc acgtaattca
    10681 tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg gatcttatta
    10741 cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt caatttttgc
    10801 agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg
    10861 tcaactagct tcccaccaaa ttctaaatat aatttgtcat agtggttcac tcgttccaaa
    10921 atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt tttcactagg
    10981 ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca actggaagag
    11041 cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga tagttattca
    11101 ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa
    11161 cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc ttgaagatcc
    11221 cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa ctcgtgatga
    11281 gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa taagataaag
    11341 gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa aaaaaggctg
    11401 taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt ataacaattt
    11461 tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag taagaggagg
    11521 caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa ctagaggaga
    11581 cgaatgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag
    11641 caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa gctgtccgcg
    11701 aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa gaattaaaaa
    11761 gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc catgatcgtt
    11821 ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg atgagctatt
    11881 acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca agtctgccag
    11941 aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa ccggagtcaa
    12001 cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct cagacagaaa
    12061 ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca
    12121 ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga
    12181 agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg cctgaaacaa
    12241 tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt cgtgcagcca
    12301 tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag ggattcggtc
    12361 aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat aatcaatcta
    12421 atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa aaatgaagaa
    12481 caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag taatcctctg
    12541 gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta
    12601 actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac tgaaaatccc
    12661 gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat tgaaacagcg
    12721 gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga ttttccacaa
    12781 ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac gatcggaaag
    12841 acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga tattggtctg
    12901 gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat caattatacg
    12961 ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga tcaacgatac
    13021 caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat caatgtccaa
    13081 gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt ctctgcaggg
    13141 cagatcacga tattcaatca caaattgaat caattattag cccatgcaag aacacttggt
    13201 tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa tcttcagagc
    13261 gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat gaaaaatcca
    13321 tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga taaaggacag
    13381 tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc atctggagac
    13441 tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc attaatggtg
    13501 agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt ctgttccaaa
    13561 ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt actaaatgga
    13621 cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa taaaatagca
    13681 cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca atttattccg
    13741 aatttccagc caatgactgc tattttttta tttatagtac tttacttaag cttaaaagat
    13801 gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg tgttggacca
    13861 tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc aatcgtctgc
    13921 ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt tctggcgggc
    13981 atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt gcctcaacct
    14041 tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg caatgttgtg
    14101 ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat
    14161 gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt tgacaagttg
    14221 cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa
    14281 agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa
    14341 aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag aagataacgg
    14401 cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct attggatgtt
    14461 tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga atgatggaca
    14521 atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta aagggcgaat
    14581 attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg aaaattttaa
    14641 aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag ttgctatcag
    14701 caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa gcatccacag
    14761 tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag ccaataaagg
    14821 acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca gtacaatcgt
    14881 cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt ttgaaaagtt
    14941 gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac tgttatttgg
    15001 tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac
    15061 tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa tactgattat
    15121 ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag gaacaatatt
    15181 gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga tcggtttctt
    15241 gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca tcggtgaaag
    15301 cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg agatcagtcg
    15361 gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg cagcaaaagc
    15421 agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa
    15481 tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag ataaatatga
    15541 cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat
    15601 ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac aatgagtccg
    15661 ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg
    15721 gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag taggattagc
    15781 ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac tgttatcgat
    15841 tctggtcttt tccggtggcg cgcagttttt agttgcaaat gatgcttacg acgcaagcat
    15901 catttgcaac gacattattg atggttttct ttttagaatt acgctataca ttattaggag
    15961 caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat
    16021 cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga
    16081 ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata
    16141 tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt
    16201 tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta
    16261 caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg
    16321 gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca
    16381 aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca
    16441 acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct
    16501 tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga
    16561 aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg
    16621 ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa
    16681 atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca
    16741 gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat
    16801 taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac
    16861 agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat
    16921 gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta
    16981 taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa
    17041 ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta
    17101 gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg
    17161 acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga
    17221 tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct
    17281 tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct
    17341 gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat
    17401 aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt
    17461 gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt
    17521 atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg
    17581 actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg
    17641 ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa
    17701 gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc
    17761 attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc
    17821 gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa
    17881 ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc
    17941 tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa
    18001 gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt
    18061 cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc
    18121 tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct
    18181 ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga
    18241 ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt
    18301 ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc
    18361 tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc
    18421 ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg
    18481 ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta
    18541 ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg
    18601 ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct
    18661 gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt
    18721 agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac
    18781 gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa
    18841 cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa
    18901 atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt
    18961 aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa
    19021 ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc
    19081 tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat
    19141 gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat
    19201 gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc
    19261 tacgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat
    19321 ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg
    19381 aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa
    19441 attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca
    19501 aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca
    19561 gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc
    19621 ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca
    19681 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
    19741 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
    19801 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
    19861 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
    19921 aagactatga taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac
    19981 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
    20041 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
    20101 tagcgatctt agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
    20161 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
    20221 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
    20281 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
    20341 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
    20401 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
    20461 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
    20521 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
    20581 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
    20641 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
    20701 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
    20761 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
    20821 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
    20881 tgaaatcgtt cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat
    20941 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
    21001 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
    21061 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
    21121 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
    21181 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
    21241 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
    21301 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
    21361 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
    21421 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
    21481 aggaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
    21541 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
    21601 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
    21661 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
    21721 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
    21781 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
    21841 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
    21901 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
    21961 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
    22021 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
    22081 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
    22141 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
    22201 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
    22261 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
    22321 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
    22381 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
    22441 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
    22501 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
    22561 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
    22621 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
    22681 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
    22741 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
    22801 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
    22861 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
    22921 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
    22981 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
    23041 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
    23101 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
    23161 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
    23221 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
    23281 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
    23341 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
    23401 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
    23461 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
    23521 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
    23581 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc
    23641 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
    23701 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
    23761 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
    23821 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
    23881 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
    23941 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
    24001 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
    24061 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
    24121 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
    24181 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
    24241 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
    24301 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
    24361 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
    24421 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
    24481 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
    24541 acgaggtctg acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat
    24601 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
    24661 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
    24721 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
    24781 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
    24841 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
    24901 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
    24961 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
    25021 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
    25081 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
    25141 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
    25201 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
    25261 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
    25321 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
    25381 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
    25441 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
    25501 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
    25561 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
    25621 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
    25681 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
    25741 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
    25801 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
    25861 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
    25921 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
    25981 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
    26041 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
    26101 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
    26161 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
    26221 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
    26281 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
    26341 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
    26401 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
    26461 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
    26521 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
    26581 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
    26641 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
    26701 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
    26761 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
    26821 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
    26881 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
    26941 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
    27001 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
    27061 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
    27121 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
    27181 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
    27241 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
    27301 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
    27361 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
    27421 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
    27481 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
    27541 tgttccatta aataaagaat aggaataccc gtggtgctag ttaattttaa tatcctaaag
    27601 aatatctcaa agttccttca aaacgtcgtt gtgctcgact taatagaaca ctgtaagcca
    27661 gtaatatgct ttcaaatcta ctctctagcc ctttaacaga acgtgagttg aaattttgac
    27721 aacctaattt ttctaaggaa gaaaaaacag tctcaactgt ttttctttgt tttccaagta
    27781 agctgtggtc tagtttttct gatttatcca tgtttttacg tggcggaacg gatatagtaa
    27841 tgcccttaag ggcaagcttt tcatggattt tatggctaat atatcctttg tcccctagga
    27901 tattgggaag attcgcttct tccgataaag tctctaacac tttcacgtca tggacacctg
    27961 gattcgtgat tgagtaagta atcggaaagc cagtcttagt aacgatcata tgaattttaa
    28021 gaccataaaa ataagatttt tttgttgagt tatagccaac tttggct
//
LOCUS       DAYYGY010000032        27867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000032 DAYYGY010000000
VERSION     DAYYGY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.032"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(419..1363)
                     /locus_tag="ACHA52_001341"
     CDS             complement(419..1363)
                     /locus_tag="ACHA52_001341"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8940069.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1659..2603)
                     /locus_tag="ACHA52_001342"
     CDS             complement(1659..2603)
                     /locus_tag="ACHA52_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8940070.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2824..4038
                     /locus_tag="ACHA52_001343"
     CDS             2824..4038
                     /locus_tag="ACHA52_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8940071.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4060..6075
                     /locus_tag="ACHA52_001344"
     CDS             4060..6075
                     /locus_tag="ACHA52_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8940072.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6158..7531)
                     /locus_tag="ACHA52_001345"
     CDS             complement(6158..7531)
                     /locus_tag="ACHA52_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8940073.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGELGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7557..7841)
                     /locus_tag="ACHA52_001346"
     CDS             complement(7557..7841)
                     /locus_tag="ACHA52_001346"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8940074.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7843..9858)
                     /locus_tag="ACHA52_001347"
     CDS             complement(7843..9858)
                     /locus_tag="ACHA52_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8940075.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10037..10342)
                     /locus_tag="ACHA52_001348"
     CDS             complement(10037..10342)
                     /locus_tag="ACHA52_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8940076.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10431..11462)
                     /locus_tag="ACHA52_001349"
     CDS             complement(10431..11462)
                     /locus_tag="ACHA52_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8940077.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11669..12544
                     /locus_tag="ACHA52_001350"
     CDS             11669..12544
                     /locus_tag="ACHA52_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8940078.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12696..13538)
                     /locus_tag="ACHA52_001351"
     CDS             complement(12696..13538)
                     /locus_tag="ACHA52_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8940079.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13630..15507)
                     /locus_tag="ACHA52_001352"
     CDS             complement(13630..15507)
                     /locus_tag="ACHA52_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8940080.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15504..16925)
                     /locus_tag="ACHA52_001353"
     CDS             complement(15504..16925)
                     /locus_tag="ACHA52_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8940081.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16928..20317)
                     /locus_tag="ACHA52_001354"
     CDS             complement(16928..20317)
                     /locus_tag="ACHA52_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8940082.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20574..21965
                     /locus_tag="ACHA52_001355"
     CDS             20574..21965
                     /locus_tag="ACHA52_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940083.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYKLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22038..22685)
                     /locus_tag="ACHA52_001356"
     CDS             complement(22038..22685)
                     /locus_tag="ACHA52_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8940084.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22693..23379)
                     /locus_tag="ACHA52_001357"
     CDS             complement(22693..23379)
                     /locus_tag="ACHA52_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940085.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23385..24458)
                     /locus_tag="ACHA52_001358"
     CDS             complement(23385..24458)
                     /locus_tag="ACHA52_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940086.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24680..25354
                     /locus_tag="ACHA52_001359"
     CDS             24680..25354
                     /locus_tag="ACHA52_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8940087.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25359..26699
                     /locus_tag="ACHA52_001360"
     CDS             25359..26699
                     /locus_tag="ACHA52_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8940088.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26848..27834)
                     /locus_tag="ACHA52_001361"
     CDS             complement(26848..27834)
                     /locus_tag="ACHA52_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8940089.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8126 a         5268 c         5051 g         9422 t
ORIGIN      
        1 tgataaacct ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc
       61 ttatgcaaaa ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa
      121 tgataaaaac cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat
      181 tgtatttgcc gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc
      241 gcttattgta atcaacagat taaatttcat atgttcatat gtacggatac attttcggac
      301 cgattaattt taaccattct gtttatttgc ttacctatta gatatcaata aaaaactgct
      361 taacagctat ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt
      421 ataatacagt gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt
      481 cttcttcgtt gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg
      541 gcacacctgt aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca
      601 atacagcttg ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac
      661 catagtaagt tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag
      721 caatcacatc taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg
      781 gtttaccacc gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta
      841 agatatagcc gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga
      901 aacgagttgt atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt
      961 cgttccaagc gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt
     1021 cgaatcctga tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa
     1081 ctaaatctaa acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat
     1141 cagcatcttt acattcttca tagccacctg accatacagc cacattttca tcgccccaag
     1201 ccataccatc taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca
     1261 atactaattc attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa
     1321 aacctgtacc gacgattact actttacgac ttgttttttt catgaataaa cagctccttt
     1381 tcataactat ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg
     1441 ttaattataa tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac
     1501 agataaatga caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact
     1561 gaaaataaat ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa
     1621 caattttcct tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca
     1681 ttgggagtac atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat
     1741 ccgatacgat tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca
     1801 aagtcgaatt ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat
     1861 ttattactaa gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa
     1921 gaagtaaaat gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca
     1981 tctgctgatg aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat
     2041 gtatagagat ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata
     2101 ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta
     2161 tgttcccctt tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt
     2221 gccaaatgat tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa
     2281 atatcctctt gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg
     2341 tcactttctt ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac
     2401 atataattga tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt
     2461 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
     2521 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
     2581 tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc
     2641 tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa
     2701 agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg
     2761 ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg
     2821 aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg
     2881 acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt
     2941 tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt
     3001 ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta
     3061 gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta
     3121 gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg
     3181 attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt
     3241 caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg
     3301 caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt
     3361 gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta
     3421 atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta
     3481 tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa
     3541 actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt
     3601 tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg
     3661 ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg
     3721 atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc
     3781 caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta
     3841 ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa
     3901 acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt
     3961 ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa
     4021 caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa
     4081 gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc
     4141 caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg
     4201 aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc
     4261 caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact
     4321 tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg
     4381 atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag taattttatt
     4441 gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa
     4501 atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc
     4561 ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat
     4621 atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat
     4681 tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc
     4741 tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt
     4801 ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg
     4861 tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa
     4921 cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt
     4981 ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc
     5041 aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac
     5101 cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca
     5161 ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa
     5221 gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa
     5281 gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca
     5341 tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa
     5401 caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa
     5461 tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct
     5521 aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag
     5581 attactactc catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg
     5641 aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta
     5701 actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga
     5761 gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga
     5821 cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct
     5881 aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg
     5941 cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa
     6001 atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca
     6061 aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt
     6121 ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa
     6181 tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga
     6241 tgattccaac tgtcaaggct atttgaccgt attctcctag ttccccaagt acaagtcctg
     6301 gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg
     6361 gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg
     6421 cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat
     6481 gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata
     6541 gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa
     6601 aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt
     6661 ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc
     6721 cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta
     6781 gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca
     6841 tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta
     6901 tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat
     6961 aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa
     7021 acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata
     7081 agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag
     7141 aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa
     7201 gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt
     7261 gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg
     7321 tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca
     7381 gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct
     7441 tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga
     7501 ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat
     7561 tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc
     7621 aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg
     7681 aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac
     7741 tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa
     7801 cttgttccta atccactttg acatactgca aaaattttca tatcattcgt ctccttttct
     7861 tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt
     7921 attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc
     7981 agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg
     8041 ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg
     8101 taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat
     8161 agctttgata taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt
     8221 gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa
     8281 ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt
     8341 taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag
     8401 cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg
     8461 tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag
     8521 agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt
     8581 ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa
     8641 taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat
     8701 cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa
     8761 tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa
     8821 cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc
     8881 gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg
     8941 ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc
     9001 catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac
     9061 atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg
     9121 ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc
     9181 caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa
     9241 actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga
     9301 aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt
     9361 taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt
     9421 tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata
     9481 cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac
     9541 tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt
     9601 cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa
     9661 tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat
     9721 acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt
     9781 tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata
     9841 actaattttg tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct
     9901 tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa
     9961 cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg
    10021 aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt
    10081 tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag
    10141 gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt
    10201 caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc
    10261 ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    10321 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    10381 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    10441 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    10501 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    10561 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    10621 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    10681 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    10741 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    10801 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    10861 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    10921 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    10981 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    11041 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    11101 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    11161 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    11221 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    11281 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    11341 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    11401 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    11461 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    11521 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    11581 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    11641 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    11701 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    11761 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    11821 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    11881 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    11941 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    12001 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    12061 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    12121 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    12181 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    12241 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    12301 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    12361 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    12421 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    12481 caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac
    12541 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    12601 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    12661 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    12721 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    12781 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    12841 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    12901 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    12961 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    13021 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    13081 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    13141 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    13201 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    13261 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    13321 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    13381 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    13441 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    13501 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    13561 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    13621 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    13681 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    13741 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    13801 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    13861 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    13921 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    13981 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    14041 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    14101 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    14161 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    14221 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    14281 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    14341 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    14401 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    14461 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    14521 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    14581 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    14641 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    14701 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    14761 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    14821 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    14881 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    14941 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    15001 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    15061 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    15121 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    15181 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    15241 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    15301 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    15361 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    15421 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    15481 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    15541 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    15601 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    15661 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    15721 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    15781 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    15841 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    15901 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    15961 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    16021 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    16081 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    16141 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    16201 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    16261 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    16321 gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    16381 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    16441 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    16501 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    16561 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    16621 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    16681 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    16741 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    16801 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    16861 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    16921 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    16981 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    17041 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    17101 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    17161 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    17221 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    17281 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    17341 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    17401 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    17461 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    17521 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    17581 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    17641 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    17701 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    17761 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    17821 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    17881 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    17941 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    18001 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    18061 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    18121 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    18181 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    18241 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    18301 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    18361 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    18421 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    18481 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    18541 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    18601 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    18661 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    18721 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    18781 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    18841 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    18901 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    18961 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    19021 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    19081 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    19141 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    19201 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    19261 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    19321 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    19381 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    19441 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    19501 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    19561 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    19621 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    19681 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    19741 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    19801 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    19861 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    19921 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    19981 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    20041 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    20101 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    20161 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    20221 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    20281 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    20341 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    20401 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    20461 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    20521 gggttgaggg agaaaaacat atgatcaggt tgttggaagg aaactataga aagatggaaa
    20581 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    20641 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    20701 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    20761 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    20821 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    20881 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    20941 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    21001 tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    21061 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    21121 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    21181 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    21241 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    21301 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    21361 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    21421 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    21481 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    21541 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    21601 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    21661 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    21721 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    21781 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaaac
    21841 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    21901 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    21961 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    22021 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    22081 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    22141 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    22201 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    22261 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    22321 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    22381 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    22441 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    22501 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    22561 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    22621 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    22681 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    22741 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    22801 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    22861 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    22921 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    22981 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    23041 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    23101 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc
    23161 agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga
    23221 aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc
    23281 ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa
    23341 tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct
    23401 gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca
    23461 atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg
    23521 cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc
    23581 ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt
    23641 gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg
    23701 accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg
    23761 gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc
    23821 gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt
    23881 tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac
    23941 gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa
    24001 ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga
    24061 ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt
    24121 aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg
    24181 attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct
    24241 cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct
    24301 tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct
    24361 gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt
    24421 aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag
    24481 attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac
    24541 ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca
    24601 tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc
    24661 aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc
    24721 caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag
    24781 tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat
    24841 gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc
    24901 agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg
    24961 tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc
    25021 attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag
    25081 ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa
    25141 agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca
    25201 gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta
    25261 tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt
    25321 acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa
    25381 agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt
    25441 tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat
    25501 tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag
    25561 gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat
    25621 taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg
    25681 agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg
    25741 atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg
    25801 ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca
    25861 tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta
    25921 tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta
    25981 gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg
    26041 cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta
    26101 ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga
    26161 cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc
    26221 agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac
    26281 catttcaaga gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat
    26341 tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg
    26401 cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag
    26461 aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga
    26521 tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc
    26581 ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca
    26641 attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat
    26701 gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat
    26761 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac
    26821 ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac
    26881 atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac
    26941 atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac
    27001 atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt
    27061 tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac
    27121 cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt
    27181 tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc
    27241 tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc
    27301 aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa
    27361 gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc
    27421 cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg
    27481 ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg
    27541 atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt
    27601 atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt
    27661 ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt
    27721 ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa
    27781 tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat
    27841 tcctcctagc aaatagattt atgattg
//
LOCUS       DAYYGY010000033        27849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000033 DAYYGY010000000
VERSION     DAYYGY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.033"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..918
                     /gene="purC"
                     /locus_tag="ACHA52_001362"
     CDS             190..918
                     /gene="purC"
                     /locus_tag="ACHA52_001362"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8940090.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            918..1172
                     /gene="purS"
                     /locus_tag="ACHA52_001363"
     CDS             918..1172
                     /gene="purS"
                     /locus_tag="ACHA52_001363"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8940091.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1174..1845
                     /gene="purQ"
                     /locus_tag="ACHA52_001364"
     CDS             1174..1845
                     /gene="purQ"
                     /locus_tag="ACHA52_001364"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8940092.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1846..4068
                     /gene="purL"
                     /locus_tag="ACHA52_001365"
     CDS             1846..4068
                     /gene="purL"
                     /locus_tag="ACHA52_001365"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8940093.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4053..5492
                     /gene="purF"
                     /locus_tag="ACHA52_001366"
     CDS             4053..5492
                     /gene="purF"
                     /locus_tag="ACHA52_001366"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8940094.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5515..6567
                     /gene="purM"
                     /locus_tag="ACHA52_001367"
     CDS             5515..6567
                     /gene="purM"
                     /locus_tag="ACHA52_001367"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8940095.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6564..7142
                     /gene="purN"
                     /locus_tag="ACHA52_001368"
     CDS             6564..7142
                     /gene="purN"
                     /locus_tag="ACHA52_001368"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8940096.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7139..8674
                     /gene="purH"
                     /locus_tag="ACHA52_001369"
     CDS             7139..8674
                     /gene="purH"
                     /locus_tag="ACHA52_001369"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8940097.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8690..9964
                     /gene="purD"
                     /locus_tag="ACHA52_001370"
     CDS             8690..9964
                     /gene="purD"
                     /locus_tag="ACHA52_001370"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8940098.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10086..11000
                     /locus_tag="ACHA52_001371"
     CDS             10086..11000
                     /locus_tag="ACHA52_001371"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8940099.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11133..12698)
                     /locus_tag="ACHA52_001372"
     CDS             complement(11133..12698)
                     /locus_tag="ACHA52_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940100.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12691..14142)
                     /locus_tag="ACHA52_001373"
     CDS             complement(12691..14142)
                     /locus_tag="ACHA52_001373"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8940101.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14156..15172)
                     /locus_tag="ACHA52_001374"
     CDS             complement(14156..15172)
                     /locus_tag="ACHA52_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8940102.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15392..16480
                     /gene="chvE"
                     /locus_tag="ACHA52_001375"
     CDS             15392..16480
                     /gene="chvE"
                     /locus_tag="ACHA52_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8940103.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16534..18060
                     /gene="mmsA"
                     /locus_tag="ACHA52_001376"
     CDS             16534..18060
                     /gene="mmsA"
                     /locus_tag="ACHA52_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8940104.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18062..19267
                     /gene="mmsB"
                     /locus_tag="ACHA52_001377"
     CDS             18062..19267
                     /gene="mmsB"
                     /locus_tag="ACHA52_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8940105.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19525..20502
                     /gene="guaC"
                     /locus_tag="ACHA52_001378"
     CDS             19525..20502
                     /gene="guaC"
                     /locus_tag="ACHA52_001378"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8940106.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20766..22190
                     /locus_tag="ACHA52_001379"
     CDS             20766..22190
                     /locus_tag="ACHA52_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8940107.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22232..22489)
                     /locus_tag="ACHA52_001380"
     CDS             complement(22232..22489)
                     /locus_tag="ACHA52_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940108.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            22651..23760
                     /gene="pdhA"
                     /locus_tag="ACHA52_001381"
     CDS             22651..23760
                     /gene="pdhA"
                     /locus_tag="ACHA52_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8940109.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23763..24740
                     /locus_tag="ACHA52_001382"
     CDS             23763..24740
                     /locus_tag="ACHA52_001382"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8940110.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24765..26408
                     /locus_tag="ACHA52_001383"
     CDS             24765..26408
                     /locus_tag="ACHA52_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8940111.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26416..27822
                     /gene="lpdA"
                     /locus_tag="ACHA52_001384"
     CDS             26416..27822
                     /gene="lpdA"
                     /locus_tag="ACHA52_001384"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8940112.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         9059 a         4787 c         6332 g         7671 t
ORIGIN      
        1 taaatgtaaa cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa
       61 aagaatctaa tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa
      121 atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga
      181 gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt
      241 acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc
      301 gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt
      361 ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc
      421 gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca
      481 gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc
      541 attgaattct actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc
      601 tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca agcgttggct
      661 attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag
      721 ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact
      781 tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat
      841 ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa
      901 gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt
      961 tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg
     1021 aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg
     1081 atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc
     1141 gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca
     1201 ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc
     1261 gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga
     1321 ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg
     1381 gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc
     1441 cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat
     1501 tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat
     1561 gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac
     1621 gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac
     1681 atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga
     1741 atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg
     1801 ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa
     1861 gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat
     1921 gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact
     1981 ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta
     2041 cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc
     2101 gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca
     2161 gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc
     2221 agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat
     2281 ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt
     2341 atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca
     2401 ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc
     2461 gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac
     2521 ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta
     2581 caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg
     2641 catcattcgg attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct
     2701 agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca
     2761 caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg
     2821 ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat
     2881 ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa
     2941 ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat
     3001 aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca
     3061 gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca
     3121 aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca
     3181 ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca
     3241 gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca
     3301 gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg
     3361 aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga
     3421 attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat
     3481 aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt
     3541 gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata
     3601 ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga
     3661 atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta
     3721 ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg
     3781 gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa
     3841 ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg
     3901 gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg
     3961 acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca
     4021 gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg
     4081 aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg
     4141 ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag
     4201 gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa
     4261 aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca
     4321 atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct
     4381 tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg
     4441 gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg
     4501 aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg
     4561 cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc
     4621 cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat
     4681 tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca
     4741 atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga
     4801 tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg
     4861 cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg
     4921 ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc
     4981 cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc
     5041 ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag
     5101 gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg
     5161 tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt
     5221 tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa
     5281 atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg
     5341 aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta
     5401 tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg
     5461 aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat
     5521 tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta
     5581 gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga
     5641 ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg
     5701 gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca
     5761 atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg
     5821 ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta
     5881 gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca
     5941 gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg
     6001 atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt
     6061 ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt
     6121 gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa
     6181 gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag
     6241 aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg
     6301 ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc
     6361 ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg
     6421 ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt
     6481 gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt
     6541 gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca
     6601 actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt
     6661 tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg
     6721 cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc
     6781 ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg
     6841 gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac
     6901 ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg
     6961 gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa
     7021 aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag
     7081 agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat
     7141 gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt
     7201 gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc
     7261 tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg
     7321 tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag
     7381 ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt
     7441 atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa
     7501 cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac
     7561 ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca
     7621 tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata
     7681 tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt
     7741 gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc
     7801 tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca
     7861 tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa
     7921 ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac
     7981 agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt
     8041 tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa
     8101 gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca
     8161 aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg
     8221 agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga
     8281 aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc
     8341 attagtgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa
     8401 cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat
     8461 tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt
     8521 tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc
     8581 aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat
     8641 ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt
     8701 ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa
     8761 ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg
     8821 tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat
     8881 tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat
     8941 ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa
     9001 aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt
     9061 tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa
     9121 agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct
     9181 ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt
     9241 cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa
     9301 ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc
     9361 aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga
     9421 aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc
     9481 tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga
     9541 attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga
     9601 ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca
     9661 agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac
     9721 aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc
     9781 ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg
     9841 tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga
     9901 tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa
     9961 gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt
    10021 agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag
    10081 ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag
    10141 gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa
    10201 acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct
    10261 ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat
    10321 gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag
    10381 aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag
    10441 atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa
    10501 aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat
    10561 atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga
    10621 tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg
    10681 tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg
    10741 gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag
    10801 aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct tggattttac
    10861 gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa
    10921 tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg
    10981 ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa
    11041 gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa
    11101 tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc
    11161 tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata actgaaatag
    11221 ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc
    11281 aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta
    11341 aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc
    11401 caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc
    11461 aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg
    11521 aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg
    11581 gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg
    11641 agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt
    11701 ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt
    11761 gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca
    11821 tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca
    11881 aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta
    11941 tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg
    12001 atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca
    12061 gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg
    12121 taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct
    12181 gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct
    12241 ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg
    12301 tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat
    12361 tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc
    12421 atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc
    12481 cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg
    12541 atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg
    12601 acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga
    12661 atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt
    12721 gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt
    12781 gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc
    12841 gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag
    12901 tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat
    12961 tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac
    13021 agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc
    13081 tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata
    13141 atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga
    13201 cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg
    13261 ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag taatctgagc
    13321 ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa
    13381 agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag
    13441 tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc
    13501 gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc
    13561 atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg
    13621 gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa
    13681 gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc
    13741 cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc
    13801 gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt
    13861 gctctctcga atatcattcc taagttcttc taggatattt tcctcattga actcatctac
    13921 ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa
    13981 agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg
    14041 cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt
    14101 tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat
    14161 aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa
    14221 tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg
    14281 ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta
    14341 gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt
    14401 tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt
    14461 ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc
    14521 ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt
    14581 cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg
    14641 agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct
    14701 gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt
    14761 tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt
    14821 cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg
    14881 attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat
    14941 cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt
    15001 tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg
    15061 attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca
    15121 tcatttggta agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc
    15181 actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag
    15241 gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta
    15301 aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac
    15361 tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg
    15421 gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc
    15481 agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat
    15541 cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca
    15601 atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg
    15661 ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa
    15721 agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa
    15781 tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat
    15841 tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg
    15901 ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc
    15961 atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc
    16021 tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa
    16081 ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat
    16141 catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac
    16201 tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac
    16261 gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag
    16321 tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt
    16381 ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat
    16441 cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact
    16501 aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca
    16561 gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa
    16621 cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat
    16681 gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag
    16741 ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa
    16801 gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa
    16861 gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat
    16921 aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag
    16981 caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag
    17041 cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg
    17101 aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc
    17161 gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg
    17221 atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga tcgttatcct
    17281 gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac
    17341 cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag
    17401 attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt
    17461 ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca
    17521 gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa
    17581 tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat
    17641 aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc
    17701 cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt
    17761 gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt
    17821 ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc
    17881 gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata
    17941 ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg
    18001 cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa
    18061 gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag
    18121 agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc
    18181 gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta accttgttct
    18241 gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca
    18301 tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat
    18361 ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct
    18421 gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac
    18481 tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct
    18541 agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga
    18601 aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt
    18661 tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc
    18721 aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat
    18781 ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc
    18841 atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa
    18901 tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt
    18961 caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc
    19021 aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg
    19081 tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat
    19141 gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat
    19201 caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac
    19261 aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct
    19321 ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt
    19381 ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa
    19441 attacatagg gcgctaaaaa aaagccttga ttaaattaat aatgttcgtg ttttgctgga
    19501 tgtgaacatc aggaggataa tgtaatgaaa gtatttgatt acgaagatat tcaattaatt
    19561 cctaataaat gtatcgtaaa tagccgttct gaatgtgata caacggtcac attaggtaaa
    19621 catacattca aaatgccagt agtgcctgcc aacatgcaga cgattattga tgaaacaatc
    19681 gcagaattct tagcggaaaa tggttatttc tatatcatgc atcggtttga tgaagcagct
    19741 cgaattccgt ttatcaaaaa aatgaaaaag cgtggattga ttacctcgat cagtgttggc
    19801 gtcaagaaag aggaatattc ctttatagag aagttggcag aagaatcgct aaatcctgat
    19861 tatatcacta tcgacatcgc gcatggacat gctaactcag ttattgatat gattcagcat
    19921 attaaaaaat atttgcctga aacttttgtg attgccggta atgttggtac accggaagcc
    19981 gtccgtgaat tggaaaatgc gggcgcagat gcaacaaaag tcgggattgg tccaggaaaa
    20041 gtatgtatta caaaaatcaa gacaggcttt gggactggag gctggcaact ggcagctctt
    20101 agatggtgtg cgaaagcagc tcgcaagccg atcatcgctg acggtgggat cagaacacat
    20161 ggcgatattg caaaatctgt ccgttttggt gcgacaatgg tcatgattgg ttcattgttt
    20221 gccggtcacg aggaatctcc tggcgaaaca aaagtcgaaa atggaatcgt gtataaagaa
    20281 tactttggtt ctgcttcaga attccaaaaa ggcgaaaaac gaaatgttga aggaaagaaa
    20341 atctggatcc aacacaaagg atctttgaaa gatacactag tggaaatgca acaagacctt
    20401 caatcatcta tttcttatgc tggaggaaga gatctggaag ccattcgaaa agttgactat
    20461 gtcatcgtga aaaattcgat ttttaatgga gacacaatct aaaaaataaa taaaatatta
    20521 ggatgttgtg aaaaagttgt cctgcattaa gtaataagaa cagccaaaca aaaatagttc
    20581 tttatatgtt ttgtttggct ggaattatta tcgcagatac aagcttttga acatcattat
    20641 tcagaataag ggactgtgat aagatttttg tcacagtccc ttatgttttg caaaaaaagt
    20701 ttttcttttt tctcgtaaat tcggtaaaat caaaagaaaa aatagacaat agaaaagaga
    20761 agcttatgtg ggaaaaagta gagaaaaaac aaggaccgat ttatcgtcaa attatggaac
    20821 agattatgag agaaatcgaa aacggaagga tcaatcctgg aagtcaactt gaatcagaac
    20881 gaaaactagg gcaacttttt ggtgtgaatc gttccaccgt tgtccatgca ttggaagagc
    20941 ttagggagct tggggtacta acgagcaaac gtggcagcgg atggtttgtt aatcagactg
    21001 aatgggggaa attcgctgtt ccaagaatcg attggcggca aatgatctct cctcgttatg
    21061 agcagacgga ctggtatgag caaaaaataa gagaagcaaa gcagatccaa aaaacatcgt
    21121 ttcttgattt atacgctagc gagatgccag atgagctttt gccgaatttt gagtttcctt
    21181 cttattctct tgaagaaatc ttagcggaag aaaaagaaat ggatctttta ggctatactc
    21241 cgttacgtaa caaaatcaaa cggtatttgg aaagtaaatt tgaattggca ttttccccca
    21301 accagcttct tgtgactggt ggcggtcagc aagcaatctt tttgatttta caaacgattc
    21361 tttcagctgg agaggggata gctgtagagt caccttcttt tttttaccga ttacctcttt
    21421 tcaaagcttc aggaacgcgt ttatttggga taccgatgga tgaagaaggg atcgacttag
    21481 aaaaactgaa agaatcgatc cataaaaata aattgaaagc agtgcttatc aacccgaatt
    21541 ttcaaaaccc tacaggaaaa gtgatgagtc aaaaacgacg agaaaagcta gtagaattgt
    21601 gtcgacagta tcggcttcca attattgaag acgatgtatt tacggatctt tcatttctaa
    21661 accaagaaga agtccttcct atacgaacga ttgatccgga taatgtgtta tacgtcggtt
    21721 cgttatctag ggttttagga aaaacaacga aaatcggttg ggtgatagga cctgagactt
    21781 ttatctcgca actagctcaa gcgcaagaaa tgatggagtt ttcgatgaat atccttacac
    21841 aaatcacagt ggctaatgta tttaatgaag caattgattt gaaaatgcaa gatgtaagaa
    21901 aaaagttgaa agaaaaaagc cgactgataa gcgcttgggg agattcacaa tcgtttttca
    21961 agcttcattc aattttaggt ggttactatg cctggattac ttgggatggg aaaaaactag
    22021 accccgaact agcagaaaaa gtagtagata tgggtcttgg aattgcacca agtctacttt
    22081 ttgggcaaga tataaatgga atcagaatca attacagtag gctggatgaa aaccaaatac
    22141 cgttgtttta ttcaaagatg aaaattctag aaaagtggtt aacagaataa tttcacaaag
    22201 taaatagatt aaagaatata acagcttgga tttaaatttc aagctgtttc ttttttttcg
    22261 gaaaaagaat acgatataaa gaaactaaga aaaaaccatt atttgtacgt ccgttcacga
    22321 tgtgtaaata aaataaatgt ttgcaacaaa actgtataac accaacgttt ttaaatagaa
    22381 acagatggtt gtaagtttga ttagaaaata gaaaaaatgc agatgaaata taaaacgctt
    22441 tccttattgt gattttattc actaatgctg ttaatatgcg gattggcata atttcctttc
    22501 atcgtgaaaa tttgtgcttt tagcttgtat ttctcataaa tccatgataa tttattaatg
    22561 aagacggtta aatagtgata cagttttatt cactgtttca atcgcagaat gaagagattt
    22621 tcttatagat gagagaggaa tgtgtgacaa atggcaaaca aaaaagcacc gatcgacttc
    22681 caagcactgc tagatgaagt caatgcagat ttcccagtat atcaagcact tgatcaagat
    22741 ggaaacgtag ttaacaaaga tttagtacct gatttatccg atgatgaact cgttgaatta
    22801 atgactcata tggtatggtc tcgtgtatta gaccaacgtt caacagcgct gaaccgccaa
    22861 ggacgcctag gcttttttgc accaactgct ggacaagagg caagtcagct ggctagtgct
    22921 tacgcatttg ataaagaaga tgttcttctt ccaggttatc gggatgtacc tcaactagta
    22981 aaacatggtt tacctttatc tcaagctttt ttatggtctc gaggacatgc tgcaggtaat
    23041 tcttatccgg aagaattgaa agctctacca ccacaaatta tcatcggtgc acaatatgta
    23101 caagcagccg gtgttgcatt aggtttgaag aaacgaaaca agaaaaatgt cgtcttcact
    23161 tatactggtg atggcggttc atcacaaggt gatttttacg aagcaatcaa tttcgctggc
    23221 gcttaccatg caaatgccgt attttatatc caaaacaacg ggtatgcgat ctctacacct
    23281 cgtgaaaaac aaactgcagc aaaaacactt gcacaaaaag ctgtcgcagc tgggattcct
    23341 ggtattcaag tcgatggaat ggatccgctt gcagtatata ctgtatctaa aatggcacgg
    23401 gaatgggcga tcagtggaaa tggaccagtc ctaatcgaaa cactgactta tcgttatgga
    23461 ccacatacac tatctggtga cgatccaacc cgctaccgta caaaagacac agatgacgat
    23521 tggcagaaaa aagatccatt gatccgcttc cgtaaatatt tgacggaaaa aggactttgg
    23581 tctgaagaaa aagaagaaca agtcatcgaa gcaactaaag aagaaatcaa agcagctatt
    23641 gctgaagccg acaaagtacc aaaacaaaag gtttcagatt tcttgaaaaa catgttcgaa
    23701 gtatctccac aatcaatcaa agaacaaatt gcaatttatg aagcgaagga gtcgaaataa
    23761 tcatggcaca aaaaacaatg atccaagcaa tcacagatgc cttagcactt gaactagaaa
    23821 atgacgagaa tgtcgttgtt tttggtgaag atgttgggaa aaacggagga gttttccggg
    23881 caactgaagg attgcaggaa aagttcggag aagaccgagt attcgatact cctttagctg
    23941 aatctggaat cgcaggattg agtttcggtc ttgcgttaga aggatttcgt ccagttcctg
    24001 aaatccaatt ctttggtttt attttcgaag caatggacga agtagtagct caaatggctc
    24061 gtacaagata tcgaatgagc ggcactagaa atctacccat cacaattcgt tcgccatttg
    24121 gtggcggtgt tcatacacca gaattgcatt cggataattt agaaggtttg atcgctcagt
    24181 ctcctggtat tcgagtagtg attccttcca acccttatga tgcaaaagga ctattgattt
    24241 cttctattag aagtaacgat ccggttgttt tcctagaaca catgaaacta tatcgctcat
    24301 tccgagaaga agtgccggat gaagcttacg aagttccttt ggataaagca gcagttactc
    24361 gtgagggaac agatgtatct attatcacat acggagcaat ggtgcgtgaa gcaatcaaag
    24421 cagcagacaa tcttgcaaaa gaaaatatat ctgttgaaat cattgatctt cgtacagttg
    24481 cgccattaga tgtagaaacg atcattcaat ccgtggaaaa aactggtcgc gtagttgttg
    24541 tccaagaagc acaacgacaa gcgggcgttg ccgcgcaagt agtttctgag atctcagaac
    24601 gcgctatcct ttcattagaa gccccaatcg gacgagtttc tgcacctgat acagtcttcc
    24661 catttggaca agcagaaaat gtctggttgc caaatgcaaa agatatcgaa gacaaagtaa
    24721 aagaaatcgc ggaattttaa gaaaagaagg gacgaaacaa agtcatggct tatcaattta
    24781 aattgccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac
    24841 caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac aaatcagtag
    24901 aagaaatccc gtcacctgtt acaggaacgg ttaaaaatgt gattgttcca gaaggtaccg
    24961 tagcaaatgt tggggatgta ttagtagaaa tcgatgcacc aggacacgaa gacaatgaag
    25021 gggacagtgg tgtagccgca gaatcccaaa ctccagctaa accggctgct gaacccactg
    25081 ttgatacaga atcagctgga tcgtcaagcg aaggagtctt ccaatttaag ttaccggata
    25141 tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga
    25201 tcaatgaaga tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaattccgt
    25261 cacctgtcac agggacagtt aaaaacgtga ttgttccaga aggcactgta gcaaatgttg
    25321 gggatgtatt agtagaaatc gatgctcctg gacacaatag tgcaccgtca actagtgcgc
    25381 catcagctga agcaccaaaa gaaaaagtgg aaacatcagg ttcagcaagt gtcgttgaag
    25441 ctgcagatcc aaataaacgt gtattggcta tgccatctgt tcgtcaattt gcccgtgaaa
    25501 aagatgtaga tatctcacaa gtaactgcaa ctggaaaagg tggcagagta acaaaagagg
    25561 acatcgaaaa cttcttggca ggtggtccaa gcagtgcacc agcgaaatca gaagcgccag
    25621 aagcggcagc accgaaagaa gcagctcctg cagcagaaag caaaccagct gcaccagcaa
    25681 aaccattcaa gtccaactta ggagacttgg aagaacgtgt agcaatgacg ccaacacgta
    25741 aagctatcgc taaagcaatg gtcaacagta agcatacagc tccacacgtg acattgcatg
    25801 atgaagtaga agtgtctaaa ctatgggata atcggaaacg atttaaagaa gttgctgctg
    25861 caaatggtac gaaacttact ttcttgccat atgtcgtgaa agctttaaca gcgactgtta
    25921 agaaataccc agtattgaat gcatctatcg acgatgcaaa ccaagaaatc gtttataaac
    25981 attactacaa tatcggtatt gcaactgata cggatcacgg actatatgta ccaaatgtga
    26041 aagatgcaga ccgcaaagga atgtttgcta ttgcagacga aatcaatgag aaagcaaaat
    26101 tagctcatga cggcaaacta tctgcagaag atatgcgcaa tggaacaatt acgatcagca
    26161 atatcggatc tgtaggtggc ggttggttta caccagtcat caactatcca gaagtagcta
    26221 ttttaggtgt aggaacgatt gcacaacaac caatcgtaaa tgctgaaggc gaaatcgttg
    26281 tcggtcgtgt gatgaaactg tcattaagtt tcgatcaccg tatcgtagat ggagcaactg
    26341 cgcaacaagc aatgaataat atcaaacgtc ttttagctga tccagagcta ttaatgatgg
    26401 aaggatgata aacaaatggt agttggagat tttgctgtag aattagatac agttgtaatt
    26461 ggtgcgggac ctggtggcta tgtagctgcc atccgcgctg ctgaaatggg acaaaaagta
    26521 gcaatcattg aaagagagta tattggcggt gtttgtttga atgtcggctg tattccttca
    26581 aaagcactga tagctgcagg acaccattat caagagtcac tagattctga aatgtttgga
    26641 gtaactagtg aaaacgtcaa actagatttt gcaaaaacac aagaatggaa agaaaacaaa
    26701 gttgtccata cgttgacttc tggtgttggt ttcctattaa aaaaacataa ggttgaaaca
    26761 atcgaaggag aagcgttctt cgtagatgat cacacactac gcgtcattca tccagattca
    26821 gctcaaactt attcgttcaa taacgcaatc atcgcaacag gttcccgtcc aatcgagatt
    26881 ccaggattta aattcggagg acgtgtgtta gattctactg gcggattagc attgaaagaa
    26941 gtacctaaga aatttgtgat catcggtggc ggtgtgattg gtgccgaatt gggcggagcg
    27001 tacgcaaact taggagcaga agtaacgatt cttgaaggct caccacaaat tttaccaact
    27061 tacgaaaaag atctagtaaa attagtagaa gatgatttca agaaaaaagg cgtaacagtc
    27121 gtaacgaatg caatggcgaa agaagcagtc gataacggtg acagcgtgac agtgaaatat
    27181 gcagtggacg ggaaagaaga atccgttact gctgattatg tgatggtcac tgtaggtcgt
    27241 cgtccgaata cagatgatat gggcttagaa caagcgggtg tcgaagtagg agaacgagga
    27301 ttgatcacgg tggataaaca aggacgtacg aacgtaccta acatctatgc catcggtgat
    27361 atcgttccag gggcggcatt agcacacaaa gccagttatg aagcaaaaat cgctgctgaa
    27421 gcaatctctg gcaaaaaagt agctgtagat tataaagcaa tgccggcagt agccttcact
    27481 gatcctgaat tggcttctgt aggtatgacg atcaaagaag caaaagatgc tggtatcgaa
    27541 gcaaaagcat ataaattccc attttcaggg aacggtcggg cattatcgct tggtaaaaca
    27601 gaaggattca tccgtctagt tacgacgatc gaagataacg tgcttattgg cgcgcaaatc
    27661 ggtggagtcg gcgcaagtga tatggtctct gaattagctt tagcaatcga atcaggaatg
    27721 aatgctgaag atatcgcctt gacgattcat cctcatccat ctcttggcga aatcgtcatg
    27781 gatgcttcgg aattagcatt aggtttacca atccatattt gatttgaata atacaggcga
    27841 aacgcaact
//
LOCUS       DAYYGY010000034        26660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000034 DAYYGY010000000
VERSION     DAYYGY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.035"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1210)
                     /locus_tag="ACHA52_001385"
     CDS             complement(65..1210)
                     /locus_tag="ACHA52_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8940113.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(1271..1918)
                     /locus_tag="ACHA52_001386"
     CDS             complement(1271..1918)
                     /locus_tag="ACHA52_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8940114.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            2112..2405
                     /locus_tag="ACHA52_001387"
     CDS             2112..2405
                     /locus_tag="ACHA52_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940115.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            2449..2760
                     /locus_tag="ACHA52_001388"
     CDS             2449..2760
                     /locus_tag="ACHA52_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940116.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            2757..3381
                     /locus_tag="ACHA52_001389"
                     /pseudo
     CDS             2757..3381
                     /locus_tag="ACHA52_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            3522..3680
                     /locus_tag="ACHA52_001390"
     CDS             3522..3680
                     /locus_tag="ACHA52_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940117.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            3681..4043
                     /locus_tag="ACHA52_001391"
     CDS             3681..4043
                     /locus_tag="ACHA52_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940118.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            4080..4928
                     /locus_tag="ACHA52_001392"
     CDS             4080..4928
                     /locus_tag="ACHA52_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8940119.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            4918..6393
                     /locus_tag="ACHA52_001393"
     CDS             4918..6393
                     /locus_tag="ACHA52_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8940120.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            6659..7066
                     /locus_tag="ACHA52_001394"
     CDS             6659..7066
                     /locus_tag="ACHA52_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8940121.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            7069..7284
                     /locus_tag="ACHA52_001395"
     CDS             7069..7284
                     /locus_tag="ACHA52_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940122.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            7288..7668
                     /locus_tag="ACHA52_001396"
     CDS             7288..7668
                     /locus_tag="ACHA52_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8940123.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            7802..7957
                     /locus_tag="ACHA52_001397"
     CDS             7802..7957
                     /locus_tag="ACHA52_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8940124.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            8026..8499
                     /locus_tag="ACHA52_001398"
     CDS             8026..8499
                     /locus_tag="ACHA52_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8940125.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            8496..10190
                     /locus_tag="ACHA52_001399"
     CDS             8496..10190
                     /locus_tag="ACHA52_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8940126.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            10156..10341
                     /locus_tag="ACHA52_001400"
     CDS             10156..10341
                     /locus_tag="ACHA52_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940127.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            10345..11520
                     /locus_tag="ACHA52_001401"
     CDS             10345..11520
                     /locus_tag="ACHA52_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8940128.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            11513..13036
                     /locus_tag="ACHA52_001402"
     CDS             11513..13036
                     /locus_tag="ACHA52_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8940129.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            13092..13376
                     /locus_tag="ACHA52_001403"
     CDS             13092..13376
                     /locus_tag="ACHA52_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8940130.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            13363..13698
                     /locus_tag="ACHA52_001404"
     CDS             13363..13698
                     /locus_tag="ACHA52_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8940131.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            13993..14619
                     /locus_tag="ACHA52_001405"
     CDS             13993..14619
                     /locus_tag="ACHA52_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940132.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            15361..16197
                     /locus_tag="ACHA52_001406"
     CDS             15361..16197
                     /locus_tag="ACHA52_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8940133.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            16262..16696
                     /locus_tag="ACHA52_001407"
     CDS             16262..16696
                     /locus_tag="ACHA52_001407"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8940134.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(16764..18086)
                     /locus_tag="ACHA52_001408"
     CDS             complement(16764..18086)
                     /locus_tag="ACHA52_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8940135.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(18240..19880)
                     /locus_tag="ACHA52_001409"
     CDS             complement(18240..19880)
                     /locus_tag="ACHA52_001409"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8940136.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            20080..21159
                     /locus_tag="ACHA52_001410"
     CDS             20080..21159
                     /locus_tag="ACHA52_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8940137.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(21410..22549)
                     /locus_tag="ACHA52_001411"
     CDS             complement(21410..22549)
                     /locus_tag="ACHA52_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8940138.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(22858..23571)
                     /locus_tag="ACHA52_001412"
     CDS             complement(22858..23571)
                     /locus_tag="ACHA52_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8940139.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(23825..24526)
                     /gene="nagB"
                     /locus_tag="ACHA52_001413"
     CDS             complement(23825..24526)
                     /gene="nagB"
                     /locus_tag="ACHA52_001413"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8940140.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            24760..26292
                     /locus_tag="ACHA52_001414"
     CDS             24760..26292
                     /locus_tag="ACHA52_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8940141.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(26483..>26660)
                     /locus_tag="ACHA52_001415"
     CDS             complement(26483..>26660)
                     /locus_tag="ACHA52_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8940142.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         8846 a         4788 c         5320 g         7706 t
ORIGIN      
        1 aaaccttgat atgatagggt ttctcctttt tttatttttt actgtatgcc atattgtatg
       61 ccatctagtc aatatttaag tgttgtacta atttttcaac agtcttttct ttcgtttctt
      121 ttgccaagtg accgtaacaa tcgatcgtct gactaatttt tgtatgccgt aatctagttt
      181 gaacttctac catagtagct ccagcgtcca agagcattga agcatgagtg tgtcttgcta
      241 catgcacacc gagaaatgga accatagctt tttcacaaat agtaatcaat cttgcataaa
      301 tagcgtggtg tgtgattagt tctttataga taccacaaaa aatcatagtc ggatcagtta
      361 caccaagtgt cagcatatat ttccgctgat taatctgcca tttttttaat atagaaattg
      421 ttctttcatc tagtggaaga accccagcac tacaatcagt tttaggatca ctgattttat
      481 agccattttt ggattggctt agtgttttat tgatggtgac cgtattttct tgaaaatcaa
      541 tgtctgacca attcaatgca agggcttcac tgatcctaac cccgctataa aagagaaagc
      601 gtagtaacgt tttatcaaag tcgtttcgat aagtttcagg tttgctgttt aggtaatcaa
      661 acaaggtgat cagttgttct ttactgtaaa ctttgacctt ttctttatgc ttagtggtgg
      721 ttcttttagg cattaatacg ttgtctagtg gattagtctc aataatccca agattgatag
      781 cgtagctaaa aatacgctta gaagttgaat gaagtacctt gtattggtca gtagtttcag
      841 cccagctatt tacaatttgt tgggcttttt ttaccgtgac ttcattcaca cgaagatttc
      901 catattttgg aagaatccac tttctaaact tgatttcagt tttcatgaag gtggcttctc
      961 ggacagtggt tttatatgtt tctagccaca agtcataaac ttctttaaac gtttcgttgt
     1021 tcgctttttt aataccgttt ttatcgtagt ccacttgcaa gcgtgacaag gcaagctggg
     1081 cttctttttt tgttttgtaa cctcgtctac gaaccttatc aggtttgcca gtcattttgt
     1141 tcattccaag ataggcttca tagagccaaa atttaccttt tttagtttca tattgcttga
     1201 atgttgccat aatagttttc cctccatagt gtagcaagcc gtgggggcgg ttggaggagg
     1261 cagtttatta ttattttgaa tagtgtttat ttagctcagt aacaagagct gtgaactctt
     1321 ttagaagatc attttcataa aattcttgat actctttttt ctcatctgta tttttttctg
     1381 aatcattaat taatgagtca tatcgtaaaa gcgtatttag aagagctgcg agataagcaa
     1441 ttggggtact cccgtcagga aattcaattc gagatcctgt tttaagatat tttattgcgt
     1501 ttgttaaata aatcttctgt gagacagtca ggttatcttc ttcgatgtta ttgatagcat
     1561 tagaaagatt agaatcaatt atagagttat ttttttcaat agtttttttc aattgctttt
     1621 caagcacatt tcgcttaatt tcgggcatca tatgcatatc tcttaatgta taagatccgt
     1681 ttaatagata ggtagtggat acatctaata cctttgaaag ttgtttaagc ctggtgttat
     1741 tgggaagatt atagccattt tcccaattac taacggcacc tttactggcc ggtggggata
     1801 atttacctcc aagttgttcc attgagagtc ccttttcaat tctagccagt ctaattcttt
     1861 gtcctgtctc cactgcttcg gggtattttt ttctagccac attttcacca ccattcatat
     1921 attttctcta ctatatcacg atttaaatat gtgtgaaaca taaaagttca aaaaagtatg
     1981 ttaaaattct tgcaaatgaa aaggtatgat ggtatattga gtttataaga ggtatagaaa
     2041 tgtattcaag agcctcttgc atttttttaa aatctttgta tagaaatgta tgctaacgag
     2101 gggggataaa tatggaatta aaaattgaca aactcacgct tgatgatcca gcttttaaaa
     2161 ttattgacga tcgagttgtt caacaagtag agcaaactca aggaacgtta ctttcattat
     2221 tagaaaaaaa gtaccagttt ccaaaatata tgaacaaggc acaagctgct aagtatatga
     2281 acgtttcata caatactatg atgcagaaat atgttcctaa cggtttaaag ttgattatag
     2341 ttgatggagt cgtaagaatt tcacaagatg aatgtgatcg ctttatggaa gaataccaaa
     2401 aataaacgca agccgtgggg gcaaagggat taataggagg aaataataat ggaatcgaca
     2461 caaaataaag caatcgaaaa agtattatca acaattctta cagaagacac agcggcagaa
     2521 ttagcaaact tggaaggcaa agcgctagaa gaaacgtttg aatgtttgta cgaacaaatg
     2581 gactatcaaa aattgctacc acaagaacca acggtaagcg gagtattgcg tggcttgaac
     2641 gatttagtac aagcagagtt taaagaacga ctttctattg aagagtatca agaaattttg
     2701 tatcagcaag ttgagttgtt agcgagcttg ctagggcttg aactggagga cgttgaatga
     2761 aaactgaaat ttggaacgga cacattataa gatttgtcga tattaatgat gaatggtggg
     2821 cagtggcgaa agatgttgcg gaagcgttag gtcttaaaca ggttactaga gccattcact
     2881 ctttgcctaa agatggggtt actactagta aggtcatcga ttcattgggc agaacacaag
     2941 atgtaaacat catcaacgaa aaaaaatatt taccgcatgg cattcaaaag ccgtaaaaaa
     3001 gaagccgagg catttcaaga ctggattttc gacatcatca aagaactacg ccagtccaca
     3061 ggacttgaag gctttcaagt attccgcatg ctagacaaag aacaccaaaa agaagcaatg
     3121 actaaattaa gccacgctat tactgaacca aaaccagtag actacattaa agcgaatgtg
     3181 atagccaata aagctgtatc aactatttat ggtcattcca aaatggtaaa aaagaaagat
     3241 atgactcctg aaatgttggt tgatcgtgaa cctattttag atgagacagt agaattaatg
     3301 actgttaaag aaaaatatgg gttgcagttt tcagtgagtg agaaaattta taatcgttct
     3361 gctgaattgc agacaactta ggagcattaa catgatcgag aacaaagaaa aagccttatc
     3421 cataacagtc ttggcggaca ggataaaggc aacttaattg ggataaaacc cgatattctt
     3481 tgttccgatt ataccatatt aaaaaatcgg agggaaccat tatgaaacga aacttaatta
     3541 taggaagcat tttaatcggc ggaattttcg gcttattacc attaattgca aaaatagcta
     3601 tctttgcagg attagcgtta tatctcttca ctgcttatga cgagtacgac tatcaattaa
     3661 gaactggagg gcataagtag atgtacaaaa caaaactatt aaaccaactc gatagccttg
     3721 aattagagga aattaatcaa gggatcgctg aactagaaaa taatatcggg aaaacctact
     3781 ttggaaattc attcaatgaa aaactaacag ttttgtatgt actcaaaaaa catgcagaac
     3841 acaaaataat ttgtcgagaa atcaacgagc taaaaaatca gattttaacc gcatggttaa
     3901 acattactga tatgcaagaa gcaagggtga aaacatttaa tacatgggta aagtatcaga
     3961 atcaattaaa aggtgctgaa tttgttcggg atgggttgaa atacgaactt gagcagttga
     4021 agctcatgga ggtgtctgaa tgatttatac cagtattttg aaaaaggagg agaggtttat
     4081 tggcaaataa ttatcaagca gcaacggaat tacaaaagtc gggatggtca atttatccct
     4141 tatctcctgg aactaaaatt ccaataaaag gctctagtgg ttggaatgat gcaactaacg
     4201 attctaatca aattaatgaa tggtggacgg aagatgcaac tagaaatatt gggttaatgc
     4261 ttgaagatca tcaaatggtg gtagttgacc tagatcaaca ttctgaaaca cgaaacggcg
     4321 ttgagaatta taaaaaactt gcttcaatgt atgatccatt tccttctact tatacggaaa
     4381 tcacaccagg taaaggaatc catttcttct ttaaaattcc tgatggtgta attatccaac
     4441 aagaaacagg tgcatttgct gatatcttcg ggcgtgatga taaaggtaaa agtttaagcg
     4501 ggattgatat tatcactaaa ggagttccaa tagcgccaac tatgattatt tcaagaagtg
     4561 ttgggagagc atatgaacct attgaaaacg tatctctaaa taatattgca ccagctcccg
     4621 attggctagt gaacctactg ctaaaaaaac cgcaagaaat aaagactcaa taccgacccg
     4681 tagcaaaaac agggacagca aaaaaattag atatgattgt tcaaggcgct ggcgaaggta
     4741 gtagaaacga tcatttaact aagttatgcg gttggttact atggcacggt gtagataatc
     4801 aaactttagc agaattgatt tatacagcaa atgcgtacaa tgaaccaccg ctaaaagata
     4861 aagaagtgaa tcaaattatt cgatcgatga ttaaaaaaga tttgaggggg aaagctcatg
     4921 gctcataagc ataaattaga tagtatttta gactttcccg aagccagcga gcgtgaagac
     4981 aatatcattg aattgaaaac ctggatgtca cgattaagat gcaataaaga cgatcagatt
     5041 aaaagtaaca gtgttgtgaa tgcagaattg attttaacca atgatagcaa tttagctgga
     5101 acaattgctt ataacgaatt tagcggatac attcacctat taaaagattc tccttggatc
     5161 aatcgtaccg ctggagaatg ggaagacagt tttgaggatg ccttaacggc atatattgaa
     5221 gaaaactaca atgtagtttt tgatgacaac aaaatacaca aagcagtagt aaatgtggct
     5281 agaaagaatg ttttcaaccc agtaaaagaa cgtatcgaaa aagttaaatg ggatcaacag
     5341 cctagactgg aaacactgtt tattgacctt ttaggggtag acgacaacct ttacacacga
     5401 gaagtaacaa aacgttggat agtcggatca gtcgcacgta tctataaacc aggaataaag
     5461 tttgagatcg ttccagtttt ggatggaccg caagggattg gtaagagtac cgttccagca
     5521 ttactttaca cggatgattt tttcaccgat tcattagatt cgctaggtga aaagaaagat
     5581 gattatatgc agcttcaagg caatgtaatt attgaacttg gagaattatc ttcaatgaac
     5641 aaaactaaaa ttgaacagat aaaaaacttc atcagtgcca agatcgataa aattagacct
     5701 ccttatggtc ggaatgtcat agcttgggca aggcaatgtg tatttattgg aacgtcgaat
     5761 gatgggcagt atttgaaaga tgatacagga aaccgccgtt tttatccttt gccttgtaag
     5821 aataagccaa agatgaatcc gtttaaaaca aaggatgaat attttcttca agtgttagct
     5881 gaagcaaagg tgctttttga taaaggacaa cgaatctatt ttagtcccga tgaagataaa
     5941 gaagtcttag aaattgcaaa agactatcaa gaggatgcca agatggagaa tccaattaaa
     6001 gaagcaatca ctaaatattt ggaaatggaa atcccgtata aatgggaaga agctccagcg
     6061 tgggcgagac gttcttatta tcagaactat cctgacagta aatgtaatga gaaaaattta
     6121 cagcatttct ataatggagc tactatgcaa caagagtttt atttagttga tagcgtactc
     6181 accgctgata ttttggaagc tgtttttgat aaacaagcaa aggatttatt gaatggtcga
     6241 tcagatgcag aaactaaaaa gattgccttg attattagca gtattcctgg ttgggaacgg
     6301 aagcaactgc caaaaagaaa taaacgacga ggattttaca ataagtcaaa tgcctatgaa
     6361 aataaaaatc gagctggaaa gtacaaaaag taacgggtag taacgggtca gtaacaccta
     6421 gcccgttact agtttaccct tactgcccca agggatacag caatttggta acggggtaac
     6481 accttatttc cttaaaactt ttgaaatagt tagctaatat gctggggaat aatgttactg
     6541 gattttaccc gttacctgtt accaaaacgc tagaaccctt gtccccgttg ggattgagtg
     6601 gtaacgggta cctgttacgt aggtgttacc acgtgttacg aatcaaggag gaaagtaaat
     6661 gctcaatatt atttcaacaa acaaagcacc gaactttcaa tatacagatg aaatggatcg
     6721 gtttctaatg aacacgctgg catttagtgt gggattggta acagaagatt attccacatt
     6781 tgatccagaa gtattaaaga taatggaaga agaacctgat tggctgcagg agtcagtagc
     6841 gtggtgtcaa tcgctagtag tgggatcatt ggtagatagt ggcaactatg atgatacagg
     6901 tgagctaatg gatgaattta attgtctgct taacctatat gatcgagcga gacaacgaga
     6961 gcttacatcg aatgaggata atttgttttt aaacatccat gataagttct tggctttgct
     7021 actaacagat gatgaattga taactaattt attggaggta gaataattat gcatatggaa
     7081 agtattagat acttcgatgg tgagaaacac attgagtatt caaacactca agaagatgta
     7141 gactttatta gttttgagga agataaaatc actaaagtta attttaaaga cggaacgtgc
     7201 ttgaagatag tttcgcctta tattgaatac aaatcaaaat gggaacaaga tgattctgac
     7261 gaacgagatc gctttggttg gtaatctatg gcagttaaaa aacaatgtaa tcatgctgga
     7321 tgtaaaacat tgattgatta taggcagaag tattgtggga agcataaagc aaagcaaacg
     7381 gcaattaagc gtgaagaaag aaagcaaagc gaaggtaaat attttcaatt ctatcaaagt
     7441 agaacatggc gaaaggcttc atacttgtat cggttaaatc atccagtatg tgaggattgc
     7501 ctagaagaag gcttgataag gaaagctgat gttgtagatc acaaaattga actaaaagat
     7561 gattggaata gacggttaga tgaaactaat tttcgttctt tatgccatgc acaccataat
     7621 tccaagacag ccaatgaaag acaaagacga gaaaagagta ccctttgagt taagggggca
     7681 tagtgtgaaa cttactgaca atcgatgcct actcattttg gtacaaataa ccgttgtgaa
     7741 aaggcataag ggtaattaca aataacgatt acacttgtaa tcacaagtga aaaagtatat
     7801 aatgaaagta ggagatttat tgaaaaatac aattgttatt actgataaaa cagggaaaca
     7861 gcgcaccatt gagttacctc attttggtca agtgaccatt caaatgcaga acggaaaaat
     7921 catttatata gataaattag ataaagaaaa attctgatcg aaaaacgaag gaatgtgagc
     7981 ttaattgctt acgttccttt ttcttttgtc tgaaaggagg acaacatggg acaccctaaa
     8041 atattacacg atacaaaagg caatatatca agtgaagaaa aagcaatccg tgtggacgct
     8101 agagaagaat tgttcaagca gcaaccatta ataaatatca cgccccctga ctggatggca
     8161 gcgagcgcta gaagtgaatg gaatcgtatc gtaccaacat taaaaaaaga ttatccattg
     8221 agtgaagcag attatggttc attggtagcc tattgtttag cctttgctcg aatgaaaaca
     8281 gccgaagccg agataagaaa atcaggtacg tttatcacat gcgaaaacgg agtaaagaaa
     8341 gccaatccag cggttcgggt tcaatcacaa gctatgagtg atttgaaaaa acaagccacc
     8401 tcactaggta tgaccttaga atcacgatca aaactagctt tgaacaaggt taaaaatgat
     8461 gaacccgaag acccgttcaa agagttgatg aattcatgaa tgattacatt gagaaagtcc
     8521 tatcaggtaa gctgattgca cccaagaaaa ttatccaagc gtgtgagcgt catataagcg
     8581 atttggagcg ttctaagtca gatagcttcc cttatgtgtt cgatgaagaa caagccacca
     8641 aagcgattaa attcattgag ttgctaccat ctacggacgg taaagcaatc aagatgttag
     8701 gatttcaaaa attcattcta ggcagtcttt atggctggcg tactaaagaa gggaattaca
     8761 ggcgattcaa tcgagcgttt accagtatga gccgtaagaa tgggaaaacg tatatcgcaa
     8821 gtggcatggc tgccaatgcg ttgattatgg aacaagaacc agcagaagca aggcaagtat
     8881 tgtttgtaag taacgctttg aaacaagcta aattgggcta tgatatgctg tctaattcac
     8941 ttagaaacgt ggtcaagtct agtaagtttt taagaccaca actgaaaatt atgaactcta
     9001 agattcagca cttgccctct aattcgttcg ctatggcact ggctagtgaa accagcacgc
     9061 tagatgggtt tgcaccaaca accgcaattc ttgacgagtg gcacgaagca aaaactcgta
     9121 aaacgtacaa cgtcattaag tcaggaatga cccaacaaaa gaacggctta ttgtgtgtta
     9181 ttagtaccgc tgggcttgat ttaaacgttc ctatgtacga ggaatactta ttgttagatc
     9241 gtgtgctaaa aggcgaagaa caagccgaca ggtactttat agcgatatgg gaattggacg
     9301 atcccgaaga aattcacgat caagagaaat ggatcaaagc caatccgatt tttgaaagtg
     9361 aagaaatcaa aaaagtaatg attccaacca ttcaagatga tgtgaacctt gctttgaaac
     9421 aaaataacct taattctgta ttggtgaaaa acttcaattt atggagacaa gcgagtgagg
     9481 acagctatat gattgctgaa gactggcagg caaccgaagt agaaccacaa gatattacag
     9541 gcaagcccgt ttatatcgga gtggatttat ctaaaacaga tgatttaact agcgtttcat
     9601 ggatcgtacc gctagataac ggcgaacttt attgtgattc acatagcttt gtagccacca
     9661 aatatgggct tcaagacaaa gaaaagcgcg acggtttgcc ttatcgagaa ctagaaaaag
     9721 ctggtgagtg ttccattact caattagaaa gtggaatcgt ggactatgac caagtatttc
     9781 agtttattca agatttgatt caagaaaatg atttggaatg tatggggatt tgttatgacc
     9841 cgtataacgc taattcgctt atcagtaaag ctgaaaaagc caactaccca atgttagaag
     9901 taagacaagg aacgattact ctaaacgttc cgacccgaac ttttagagaa caagtttatg
     9961 aaggcaacgt tattcacaat aaaaatacga ttctcaccca tgcagtgaac aacgctattt
    10021 taaaaacgga taacaacggc attcagatta ataaatcaaa gaacagtaac aaaattgatc
    10081 caatagctgc attaatcaat gcctatgtgt ttgcaatgga ttacttcacc acaacggaag
    10141 gagcgaaagc agacaatgaa ttttatacaa gtgaagaatt ttctttctaa ttacattcat
    10201 accgttcttt tacttcttgg attggtgtgt gtgttggttg ctatcacctt actaacaaat
    10261 gtctactatg gcttgttagc gctgggcata gtgcttattg gggtagcggt catgctaaat
    10321 acagaacaga agggaggtta aaagatggca ttttttaaag cgagacaaaa tacaacggga
    10381 gatcctttcc tacaacatgt ggtatcaatc caatcggatg attacaccac cagctttaca
    10441 agcgttcgtg cattaagaaa tagtgatgtg tttgcagccg ttcgaattat tgctagtgat
    10501 attgcttcaa gtccgattca attggtcaaa aacaatatgc cacaagctga taatgaactg
    10561 gtgaagttac taaacgagaa acctaattca gaaatggatg gttggcattt aaagtttgct
    10621 ttggcggtca atatgttgct aaacggtaat agctttgcag agattaaacg caacggtgaa
    10681 aaggtagaag aaattcactt attacctaac tcaagtgtaa cggttactca attagataat
    10741 ggtacgttgt cttatcagat tggtgataaa aaaagacgtg tgaagtctag cgatattttg
    10801 cacttcaaat atttcaccca agatggttta acaggattac caccacttta tgcgttacgt
    10861 gatgaactga aaattcaaca agctggcaac cgcacattgc acaatttctt tactcgtggt
    10921 gtcagtgggt caggtatttt gaaggttcat aagtcagatt tagacggatc agcaaagaac
    10981 gcaattcgtg aaaagtttga agaagccaac ggttcaagta gtggagataa tgctcttaga
    11041 acgatcattc ttgatgaaac aatggactac aaaacattag aaataaacac cgatgttttg
    11101 aagcttatca attccaatga ttggaacaca aaacaaattg ccaaggcgtt tggtgtaccg
    11161 attgagcgtt taggcgttga aaatgaacat tctagcaccg ttcaaagtaa tctccagtat
    11221 atccaaagta cgctgattca ttactttaat gtgtttgtga gtgaattaga tacgaaactt
    11281 gaaaccaata tccgctttaa ttccgatcag ttactagaga ctgatcctga aacaaaagta
    11341 aaaaatgtat tggatcaggt caaagggtca cttctcacga ttaatgaggg gcggtcgaaa
    11401 atggggctac cccccatgga tggtggcgat cgtttactag caagtttgaa ctttacgtat
    11461 ttagatacgt tggagaaata tcaattaaaa gaacaggaag gagttacacc agttgaataa
    11521 tgaagaagaa aaggaaaaac ggctgacaga agaagctgag ctaacagccg attctcccaa
    11581 agtagagaaa gaaaatgaaa aacaaccaac agacggcaaa actatttcag gctatgcgtt
    11641 gaaattcggg caaccgtcaa aagatttagg cggctttgta gaagtgatta cacccgaagc
    11701 attaaaagag gtggatttat caaatgtgtt cttattatat ggacacgatt attcaaagcc
    11761 actagctagt gtcaaagcag gtacgttgaa attgaatgtc gatgatacag gattacattt
    11821 tgaagcagag ctaaccgaca cgacctattc aaatgatgtg tatgagaata tctcaaaagg
    11881 ggttgttgat tctatgtcct ttgggttcgt gcttggatta gattcattcg acaaaaaaga
    11941 agatggcaca attgaacgat caatagataa aatcaaagca cttaatgaaa ttagtgtcgt
    12001 gaccgttccc gcttatgatt catcaaatgt ccaagtaaat aagcgttctt acgaatcgtt
    12061 tatgagtaac aaccaagcaa aacaaacaaa caatagctta gaatccactt ctaaagcaca
    12121 aaaggagagt aataacatgg aaaaaacttt aatcgataac gaaaaaacag aattgcgtgg
    12181 gtatgaagaa tatatccgtt cacaaggcga agtgcgtgat ggagtcacta ctgtaaatgc
    12241 agcggcagtt gttcccgaag aagtaatcgg tgaagtcttt gatttgaaac gttcaaatta
    12301 taacttagct caatatgcaa cagtaaaaac agtatcaaat ggacaaggta aatatccagt
    12361 agcaactaac caacaagcag tgttagcaac aaaagctgaa cttgctgaaa ttggtgatat
    12421 tgacgctgaa atgtttactt cagttgatta taaagtagaa actcgtgctg gtaagattgc
    12481 cttatcaaat gaggttgtgg aagattcagc agtgaatatt gtacaagagg tcaaagatca
    12541 gttagcgaaa ttggtagaaa acactgacaa taagcatatc atggatttat taaaaacatt
    12601 cactaagaaa acggctgcta cgttggacga tttgaaacaa ctatacaatg tggcattaga
    12661 cccagcatta aataaaatgg taattctaaa ccaaagcgga tattaccacc tagatacatt
    12721 gaaagattca gatggacgtt acattttaca acccgatgtg acagcaccta gtggtaaatc
    12781 attattcggt atgccagtag tattgattgc agatacattg tttgccaatc ctaaagctgg
    12841 aacattccct atgattatgg gggatattgc acaatctatc tttgttgctc gtagaaatca
    12901 agtaacgact caatgggaaa aattcgatta ctactcacaa ggacttgcag tgatcgttcg
    12961 caacgattat aagaaaattg atcaaaatgc ttcagtgtat attgagttta cgccagttgt
    13021 aacaccaaaa gtatagaaaa atattgggcg gcggtttatc ccactgcctt tttttcatta
    13081 ggagagataa aatggtaatt ttggatagca taaaaaaaag tatgcggatc gatcacacta
    13141 ttgatgatga ctttattcaa caattgattg atacagcagg cgaatatata aaaagtgcta
    13201 ttgatagtag cgcaacggat aaagatatgg ataattatca gcaatttgat ttggcggtgt
    13261 cattacttac tcaacattgg tatttgaacc gtcaagaagc cagcagcgaa cggataccag
    13321 taacggtaca agcattagta caacaaatga gaggtgctta ttatgccaat cattaagaat
    13381 gtaaatgaat tgactgagag agtcaagttt aaaaagacaa aacgggtaaa agatgaagat
    13441 gggcagatgg tggatagtga agaaactgta tttgaatgtt gggctaatgt gcgttcacaa
    13501 atgctaaaag acgtgcttgc tagtgtaggt accattcttg aaggaacgtt gacgtttatt
    13561 attcgatacg atcaagatta tgaactaaca aacgatatga aagtagcttg gaaaaacaag
    13621 agttataaaa ttatttcaat caatgaagga acggcgttta aagattatac aacgatcata
    13681 gctaaacaga tttcttagaa tgattacact tgtaaacgtt gtgggtattt agtataatta
    13741 aggtagtaaa tgaagggtta gctgcctgaa ttttgcaaga cttagctagt cgaaatctat
    13801 tgttggactg aaaattgtag gtgtggttgc aaacatgctg gactaagaaa gatgatagat
    13861 gatgaacgtt caattttaat acaggcatgc taggactgat tggatggttt tggcaagctg
    13921 attgtgtaga aatcaaagaa cacgtctgtt gtggacgtgt tttttttgtt ataattcaaa
    13981 agaggtgatt taatggacaa agcagagttt acaaattttt attggaagta ttatctacat
    14041 ttagaaaatg agtttattaa taccacagat tttgtgatgc tcgatgagaa taatttcaaa
    14101 acattctcta ttgaatatca aaaactacta ctagcgattg gttcggagtg tgagattatt
    14161 ttcaaagaac tttgcggatt tgatagcagt agcaataaaa gcataactga ttttaaagct
    14221 gttattaaat ctagcgaatt aatgtgtttg gataatggtg ttagggttat gaattcttta
    14281 acattgactt cactaaaacc ttttggagat gggtggcctg aagatacacc agattggtgg
    14341 aaaatatata acagagtaaa acatggtcgt tcttcaaatt ataaggaagc aaatttggaa
    14401 aatgtgttgt atgctttagc aagtctatat ttattagagg aatatttgca taagaaagta
    14461 atactagaag gtgaagtaga ctttataagt ccagagtcaa atctatttac gttatcttgg
    14521 gaaagaaaac attcgtcgat gcaaaaagtg acgtttgaaa aagtggattc aaattatgta
    14581 gctccactca ttgattttaa agaacaagaa gaaatttaaa aacccccacg gcttattgaa
    14641 gtttttgtat gccatattgt atgccatttg gtttaaatcg cttgaaaaat agtgatagat
    14701 aactctaatt catttagtca taaacgctgt tttgataacc tttatagttc tttgatatgg
    14761 gtttctcatt catggcggaa gaagaagaat agaacaaaag aatgaattaa taagatataa
    14821 aaaaagggat tgtgacaaac gtcttgtcac aatccctttt tccaaataaa cgatgttcaa
    14881 tcagctgccc ctcggcattc gctcgaaaaa cggcgaaata tgaagaacta ttttgccatt
    14941 tgtttctcta ataatcaggt caaaacgttg acttcacagt ctcttcctta aacggtgttc
    15001 tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgttttttg
    15061 aaattgttcc ttgtgactcg gagctgaacg gcaggctcac gacctctttc ttaaacggtg
    15121 ttcaaaagct tgcatctgcg gtaataagtt caaccaaacg aaaattatga ggagctattt
    15181 ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc ttttcgttta
    15241 gcactaaatt tccttaaaaa gtagaaaagc tttcgaagcc tcgtttgttt cttttactgt
    15301 ataataaaag aaaagagtaa caatcgctaa aagatataaa caagagaaag gaaatacaag
    15361 atgattggtc tgatttattt tgtggtgatc gttctggcga acacagttgg cgctatttcc
    15421 gggatgggtg gaggcgtgtt gatcaaaccg attcttgatc ttatcggcgc ccattctgtt
    15481 gcagggattt ctttttactc aacagttgcg gtattcacga tgtccattgt ttcgactgtg
    15541 cgacaagtat caagcgggaa atcactgaat tggcagattg tcggttgggt atctagtgga
    15601 gctgttgttg gaggagtagt cggcaacatt gtattcgaag tttttttaca gttatttgaa
    15661 aacgagaagc atgtgcagct gatccaaatt tttttgacgg tattgacctt ggtttttgcg
    15721 ttcttctaca ccaagcatca tcagccaaaa tttcatttga cgtcatggac ttggtacctt
    15781 atatgtgggg gggttctggg ctttttagct agttttttag gtattggtgg tggaccagtc
    15841 aatgtttctt tgttgatgct gatgtttgca ttgccaataa aagaagcaac actatattct
    15901 ctatctacga ttttcttttc tcaactggca aaattggtaa cgattgcttt aacaagtagc
    15961 tttatgcgct ttgatttatc tatgttgttt tatgttatcc ccgctgctgt tattggaggt
    16021 ttgtggggag caagatttag cagggttttg tctccaaaaa aagtgacctt tatttttcaa
    16081 gtaattgtcc tcgtggttct tttggtcaat tgttacaatg cctttgtcat cttacaaaca
    16141 ttaggagaat ctagactaac gcaagtcaaa atcactgaaa catattgttc taaatgaaaa
    16201 aaagagtata ctatgataaa aaaattcatt ttaaaaaatc agaaattggc tggtgatttg
    16261 gatgttacgg gaaatgcgtt tagaagatgc agaagcacta cgtgaaatca atgcggatca
    16321 attagggtat gatataccat taaccgttac ggtgagacaa atgaagaaac tgctgaagga
    16381 gccagagaaa aattttttct tggtttatga agatcctact tctaaaaaag cagctggcta
    16441 tgtacaagcg gagtcctatg aatcgatttt tagcgatcca atgttcaata ttatggcact
    16501 tgctgtgtct aaatatgctg aaaaaagagg catcggaaag gctttgatga ctggcgtaga
    16561 agaagaagcg aaaaaacgag gaataacgac aataaggctt aattctgcag aatacagagt
    16621 agaagcacac caattttatg aacacatcgg ttatcattcc gataaaatgc aaaaaagatt
    16681 tttgaaaatc ctgtaaatct ttatcaaaaa aatacccgat caccaagccg atacttgatg
    16741 atcaggtatt tttgtttttt tgactaagaa agtaattgca tcaagaaacc agagatgatc
    16801 aaaacgatgg caccaccaag tcgattgccc atttgagcaa aagcaataag ttccatccga
    16861 ttagctgcag ataagacggc tacatttcct gttccgccca tcgagttgtt gcataagcca
    16921 gcagtaatag ttgattcaat tggatataaa ccaaacaaac gaccgacaaa tcctgaaaca
    16981 actgaaatcg cgataacact tgtcagacat aagacaacaa attgccatgt caaagcagaa
    17041 gctaatacat tcagattcag caacgcaata ccgataccgg caagaacagc tgctgtcaag
    17101 tttttgacaa tcaagttatt gaacatgata gctgcatctt cataatactc tggtaatagg
    17161 ttggcgattt tacagatgac aacgataata atcataaatg cgtaagcatg gactttagga
    17221 acaaaatagt taccaatttg tcctaagatg aagaaagtca atgataccat caaaccaaca
    17281 cctaactgtg gaatggaagg tttcacagtt cgaggttttc catcgccaag atcgcctttt
    17341 tccattaatt taccattgcc gttgaatttt ggcattgatt gtccagcacg agcaactaat
    17401 gctgcaccga tgatagctaa gacatttgtc attgctgtcg caggaatcag gcgggaaatg
    17461 atagaagaag catccgtacc taaaacattt gcataaatcg ttgataaagg aacggctccg
    17521 gctccaacac caccagccat cgcaggtaat gagatgtata aaacggaatt tgcaaaacca
    17581 tttccgataa gcatgcccac gagtcctacg gcaaaaaagg agacgaccat tgaaataagt
    17641 gcaacgggaa taaaacgaac agaagctttc atcaatagat tacggttcat accaaggatg
    17701 ctaccagtaa tcaaggcagc aatataaaag tcccaaaagc ccatattgct gacaaagttt
    17761 tctgtggatt tcactacata ttcaggtaag atcccaaatg tagcaatcgc agcagaagcg
    17821 aagatcgcaa aaacggaacc accacccaaa taacttttga cgatagggat ttttgtcccg
    17881 ataaaatgaa ataaattacc gaaaataacc aaaactgcga ttggtccaat catattcgtc
    17941 ggtaatttcc ctaaagccat tgcaatgatc aggacaagga ccatgatcaa ataaagtggc
    18001 aaacttacgc cggtgatttt tgtggaccaa aaaccttctt ttttcttttc ctcaactgca
    18061 gttgcagttg ttgttttttg tgccattttt acagatcctt tcttattctg tatcatatga
    18121 aaaaagccaa agaaaagcga caaaagagca ggagttggct ggccgctgtt ttgccgcagt
    18181 cgcttttttc tttttagacg tcatacaaag tttctgtatg ttcttttact ataacgatct
    18241 taatcttcta aagatttata ttcaggaacc catttagcag attcaactgc ttctttgata
    18301 tccgtgattt cttcacgatt caagttttgt gcgacaactg attttgcgac agtttcagca
    18361 atcgtttgag aaaattcagt gatttttgct actggaggca agatagctgc tcctggtttt
    18421 gttacatcaa cgattccgcc taatgctcga ctagcttgag aaatgatttc agcatttact
    18481 cgtgtagctg ttgaagcaat caaaccaagt cctaaacctg ggtacatcaa agcattattt
    18541 gcttgaccga tttggtaagt tacaccatta tattcaacat cagcagcagg gatacctgtt
    18601 ccaactaatg cttttccgtc cgtccattct attaaatctt ttgcttttgc ttctgccaat
    18661 tttgttggat tagacaaagg catgatgatc ggacgtggtg tatgagcagc catttctttc
    18721 acgatttctt cagtgaatgc tccaggttgg gtagaagtac caatcatgat tgtcggatgg
    18781 atcgctttta caactgcttc taagtttgtc aattcatcag cattagtgaa atctgagcgt
    18841 ttgcgagcga atgggatttg accaggagtc aatccttctg tatcttcaaa caataaacct
    18901 tgtttatcta cttgatagaa atgttggcgt gcttcttctt caggtacacc ttgacggatc
    18961 atctcatcta aaaggatgtt tgcgataccc acaccagcag taccagcacc aaatgtcaaa
    19021 attatttgat ctttcaattg ttcaccggat atattcaatc cgcctaatac acctgctagg
    19081 acaacaattc ctgtaccttg gatatcgtcg ttgaaagtag tgatttgatc ttcgtatttt
    19141 tctaagatcg ctgcagcatt tcctcgtcca aaatcttccc aatgcaataa caattctggg
    19201 aataattcgg tagctgattg aacgaattga tcgatgaatt catagtaagt ttcgccttct
    19261 acacgagcat gacgattgcc taagtataag ggattattca ataattcttc attattagta
    19321 ccagcgtcga tcgatacagg caaaacttgt gctggattaa taccagcagc agcagtatag
    19381 accatcaatt tgccgatagc gatgtcaaca ccgttcacac cccagtcgcc catacctaag
    19441 attccttctg catcagtcac aacaatcaaa cgaatgtctc gtccatctgc cgcgttcttc
    19501 aaacttgctt tgatgttttc tggttcgtcg attgaaagaa atgcagcatc ttgcggtttc
    19561 aagaaaattt cgttgtattg ttcgattgaa tccgcaacga caggatcata aacgacaggc
    19621 atgaattcaa ctaaatgttc gcccatcaat ttataaaaca atgtccgatt cgtattgaat
    19681 agattcatta agaagatacg tttttccaaa tctgtttgtt tgcttaagta ttgaccataa
    19741 gcttgaacag attgttgttc cagtgtttgg acagtacttg gaagcatacc agtgattcca
    19801 tattttgccc gttcttcttt tgtaaaagct gttcctttat ttagaaaagg attatttaat
    19861 aagtttaacc ctgtaagcat gaatgattcc tcctaagttt taatacaaac gaagtataga
    19921 agtcagatga tgatatagtc aaaaagagca ggtatgataa attttattta tatggcggat
    19981 tttataatta acttagccat ttggaaataa aaaaacgcca cgtgaattcc atgttatatt
    20041 gaagttacca caactctcaa tagacaagga tgaattcaca tgacgcaaaa ccatgataac
    20101 acagtttcac gcaagggaaa acacctaacc tactcagaac gttgccaaat cgccatcctg
    20161 aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc tgggctgcgt gccgcagacc
    20221 ataaacaacg aaatcaaacg cgggaccgtt actcagctta accgccagaa tcagaacggg
    20281 aaggtctacg actattatca tgacgtctac gacgccgatg ccgggcaggc ggcctacgag
    20341 aagaaccgct tgaactgcgg cagacggccc ttatgggccg acacggacgc cttcattgaa
    20401 tgggcggacg acatgatgct gttggaaaaa tggtccccgg acgtggtggt gggctttgcc
    20461 agaaaacacg aattgttcga cccggccatt atccctggga ccaccaccct ctatcagtgg
    20521 atcgaccgtg gcatcatgaa aacgaagaat atggacctgc tagaaaaact gtcccgcaaa
    20581 ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg tgctagggaa atccattgaa
    20641 gagcgcccgg aggcggttgg tacccggaaa accttcggcc attgggaagt cgacacggtc
    20701 gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt tggtggaacg gcagacacgc
    20761 ttcgaggtca ttctgaagct ggccgggaag gatgccgaat cggtcaacca agcggtccga
    20821 cagctgagag aacgggctgg tgcgtacttc ggccagctgt tcaagacggt tacctcggac
    20881 aacggctcgg agttcgcggg actgcacgat gccttgcagg atgtggcaaa catctacttc
    20941 agccacccct atgcttcctg ggagcgcggg acgagcgaaa accaacacaa gctgattcgc
    21001 cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg aaaaccaaat cttgcggatt
    21061 cagcgttgga tgaacgatta cccgcgaaag attttgggct atgcaacgcc gcatgacacg
    21121 ttcgtccagg catttaggca ggaacggttg acggcttgac acgtgggccc ttgacaacga
    21181 gcctccctcg gcatggcttg tgtctggaaa gctggccggg gccagccagt cgggagacta
    21241 ccatgcctca gctcattgtc aagggcccac tcgattagaa aaattagacg gttggtggta
    21301 acgagcaacc gggtcaccat gggtggctaa cttaaacttg aaatttacga aaaataaaaa
    21361 gagtttagcg tacagatatt ttaaatgtac actgaactct tttttatgct tattcacttg
    21421 tttcttccag cacttttgga ctgtctaact tttccgaaac atgttccatt cttctcaata
    21481 aatagatttt ccggatattt aatattatcg tcttaatcac tgcatagaca ggtatgcaaa
    21541 tcagcatgcc taatattcct gcgacacttc ctgctccaat caataccaag atgatcgtca
    21601 aaggatggat attcattgat ttcccaaata acaacggctt gatcaaattt ccatctaatt
    21661 gttggatcac taaaatcgac aacgccatat acaaagcttg aaatggagag ataaacaaac
    21721 cgacaatgat tgctggtacc gctccaataa aagggccgac atacgggata atattcgtga
    21781 ttccgcaaat gaccgcaagc aacaagctat aaggctgatc gaatattctc attgccaaaa
    21841 agctcattac accaataatc aaagcatcta agatcgtact gctgatatag gcagataatg
    21901 tttcgtttaa ttcttttacc gtttggcgta actctttacg aatcgctttc ggaaagaagt
    21961 gagacaacgc atcaataaat ttgtgcccat ccttgaacat aaatagcaaa ataaatggaa
    22021 cggtaaataa caaaataaaa aattgcatca taaatgaaat gatttttgag acacttgcag
    22081 ataaactgac taatatgata ttgaacaaat tactaagaga taaatttgct gatgctaatt
    22141 gttcttccaa attgaaattt ttaaactctt cccgctgcgc caattcattt aaccatttgc
    22201 ttgtttcatc tgcatacatt ggcaagctct gggtcaattg aattgtctgt tccattaact
    22261 gaggcactac attcatgaca gttaaaacaa ctaaaatgat tacgaccaaa aacgtcaata
    22321 aaaaaccgaa cactctcggg atccgatgct tttcgagaaa aacaacgatt ggatcgaaca
    22381 tataatagat aaaaccagca acaagcaatg gtaaaaaaat tgccgaaagc attttcaata
    22441 ttggactaaa aatattcggc atttgtaaaa taaagtacac gccgatgatc gtgagtacta
    22501 gctccactgt ccagaaaaat aattttgatt gtttaaaacg tgattgcata catctcctcc
    22561 aaggtccttt gatacttcta ttctatcatt taacaaagag ttacgcgctg agattttcat
    22621 ttgcttaaaa aaaacaaaaa aaataaacat attttaatga gtagacgagt acttaaacaa
    22681 aaacacggtt tggagtagtg acaaaaatta tactgcagtc cctttttcca aataaacggt
    22741 gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg aaaaatataa ggaactattt
    22801 ttatttggct gggcttatta cttgatgtag gacatctatt ttacaacctc agatcattta
    22861 ttgcgcaata ctgactaaat gcaactttgt gatatttttg tgtacggaca actcctcgat
    22921 cacatcggca taagtgagcc cttttggaag atcaatcgta tagatatttt tgtacactcg
    22981 gtagtcatca acgaatttta cgtcaaaatt cacatcttcg attacgatat ttttctcttc
    23041 aaaataatcg gtaagaaaat ctttggtttc ttttctatgg ataaattgga tttctaactt
    23101 tttagtcgta ggtacatgga tcacgcgttt cactactgtg agtgcaatag caatcccgat
    23161 aaaactgatg atcgcaatgg aatagtaacc catgccgatg gcgatcccta atccagcaat
    23221 tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat tgtttcgtga cgatgatcgt
    23281 tcccgcgcct aagaaaccga caccactcac cacttgagca atcagccttg cttcatctga
    23341 acgaatcgtt ccagcaagct gtggatactc taaagcatct cttaatgcac cactagcgat
    23401 ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa actaaaatat gcgtacgcat
    23461 acctgctgga cgatttttat attgtcgttc aaaaccgatt gctccgccaa atagcatagc
    23521 gatccctaaa cgtaaaataa tctctggaat tgtcaatacc atatcattca tctaaacacc
    23581 ctccagtcca atatatcatg tctgaaagga gcagacaaaa aaagtgcaat ttttacctct
    23641 tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct ttttatgaat aaacggtgtt
    23701 caatcagctg accctcggta ttactcaaca aacggcaaaa tatggggagc tatttttcct
    23761 ttgtcttctc taatactcag gtcaaaacgc tgatttcaca acctctttag cctactattg
    23821 aaaattacaa cttacttgct gcttcttcat cgatgatcac aacaacattg tcgtgatttt
    23881 gcaatacact tgctggcaaa tgattcgtta caggtccttc aatcatccct ttgattgcat
    23941 cagctttgct ttcgccaaat gccatcaaga cgatttcttt acttttcata attgaaccga
    24001 ttcccattga aacagctttt gttggtacat cttctgcttt atcgaagaat cgtttgtttg
    24061 cattgattgt tgattctgtt aagtcaacta ctgaagtcgt tccgtcaaat gaagcaccag
    24121 gttcattgaa accgatatgt ccattttgcc cgattcctaa gatttggata tcgattggat
    24181 gtgcatcgat gatttgatca tactttttgc atgctgcttc cagatcttct gcttttccat
    24241 ctggtacaaa cgtttctttg aatggttttt tatcaaataa ttggacatcc atgaaatgac
    24301 ggtagctttg ttcatctgaa ccgcctagtc caacgtactc atctaagttt acagaagtca
    24361 tttcactgaa atccaaatcg cttgaagtca tttctttata taaagtaact ggtgtgcttc
    24421 ctgttgccaa tcctaatact tttgcacctt tgttcatacc ttctttgatg atttcaaatg
    24481 ctttttttcc gccttcttca gcgtttttta cacgaatgat ttccatagtg attgctcctt
    24541 tatattttgg tatagcccaa ttatagcatt ctttttttct ttttacaata attagtatag
    24601 accaaattgc atttttttca ttttcttgac aataaatagg aatccgtgtt aggtttcata
    24661 agatggttca atcaaacaat ccatgttgca tgaaaaccaa gcaagtgatt cactgtggac
    24721 gctttatgtc tgcagttttt ttatgaaaaa ggagttatta tgagtatact gacattaaac
    24781 aacattaccc agcaatttgg cgacaaaatc ctctatgaag gtgtcgatct gcaattaaat
    24841 gctggcgaac atcttgggct gattggacaa aacggagccg gtaaaagtac attgattaaa
    24901 atcatcactg gtgagatctt accagatgac ggacagatcc attggcaaaa aaatattcac
    24961 aaaggttacc tcgatcaata tgtggaagtc gatgaaacat taacaatcga agaatttcta
    25021 aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa ttgcttttct ttaccaaaca
    25081 tatagcgaga ccttcgatga ttccttgctg gaaaaagctg ggaaactcca aacggaatta
    25141 gaccaaggag tgttttatca aatcgacacc ttagttgctg agatgagcag cgggttgggg
    25201 atcgatgtct taggtttaga ttcactgttg aaaaatctaa gtggtggaca acgttcaaag
    25261 gtgattttag caaaactact tttagaaaac ccagatgttc tgatcttaga tgaaccaacc
    25321 aatcacttag atgatcagca tatccaatgg ctgacgcagt ttcttcagga ttttgacggt
    25381 gcgtatatcg tgatttccca tgaccaagaa tttcttgatc agatcaccac ccatatcgca
    25441 gacatcgagt ttggaaagat tactaagtat actggccatc taaaacaagc attgaaacaa
    25501 aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg ctcaacaaaa acaaatcgaa
    25561 aagaccgaag cttatattcg aaaatacaaa gccggttccc gatcaacgat ggcaaaaagc
    25621 cgacaaaaac aattagataa aatcgaacgg ctgacgcctc cagcttcagc aagtaaagct
    25681 gtctttgatt ttccattctc gcctattgtc acgacattag cagtggaaac aactgattta
    25741 gtcatcggat atgatcgacc gctacttgca cccatcaatt tgaccatccg ctttggtgaa
    25801 aatattgcca ttcgaggctt caacgggatt ggaaaatcta ctttattaaa aacactgatc
    25861 ggtgagattg aaaaaataag tggcgatttc cacttcccag ataacacaaa aatcaattac
    25921 ttttcacaag atctgtactg ggaaaatcca ttagaaacac cactacaata tttaagcagt
    25981 caatttccaa aagcgactat caaagaatta cgtcgccaac tagctaaagc agggttagtt
    26041 aatcaattag caagtgaacc tctcactaca ttaagtggtg gggaacaaac taaggtgaaa
    26101 cttgcacagt tgacaatgaa ccaaggtaat ttactgattc tcgatgaacc gacgaaccat
    26161 atcgatcaag aaacgaaaga aagcttacaa gacgggatac agaaatttcc aggaacagta
    26221 atcatcgtct ctcatgaaca agaattttat caagatcttg tgcaacgagt cattgagatt
    26281 gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg agctgtgaca aaagttttgt
    26341 cacaactcct tcttctgaat aaatggtggc caattagctg accttcggta ttagctcaaa
    26401 aaacggcaaa atatgaggag ctatttttat ttggctattc ttattacttg atgcagaaca
    26461 gcttttcaca acctcttttc gtctattttt tattttcgag atttctcttt cctttaaaaa
    26521 caactgtttg aaagaagaac gcacatcctt gaaaaacaag cccgctaacc gttacaccta
    26581 accagccata ctgctgccac attgtcgtcc ctagcaatga accagccgca cctccaataa
    26641 agtacataaa cataaataca
//
LOCUS       DAYYGY010000035        25572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000035 DAYYGY010000000
VERSION     DAYYGY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.036"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..2235
                     /locus_tag="ACHA52_001416"
     CDS             175..2235
                     /locus_tag="ACHA52_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8940143.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2318..2887
                     /locus_tag="ACHA52_001417"
     CDS             2318..2887
                     /locus_tag="ACHA52_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8940144.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3062..3868)
                     /locus_tag="ACHA52_001418"
     CDS             complement(3062..3868)
                     /locus_tag="ACHA52_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940145.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4006..5520
                     /locus_tag="ACHA52_001419"
     CDS             4006..5520
                     /locus_tag="ACHA52_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8940146.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5923..6906)
                     /locus_tag="ACHA52_001420"
     CDS             complement(5923..6906)
                     /locus_tag="ACHA52_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8940147.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6911..7558)
                     /locus_tag="ACHA52_001421"
     CDS             complement(6911..7558)
                     /locus_tag="ACHA52_001421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8940148.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7643..8275)
                     /locus_tag="ACHA52_001422"
     CDS             complement(7643..8275)
                     /locus_tag="ACHA52_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8940149.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8291..8842)
                     /gene="coaC"
                     /locus_tag="ACHA52_001423"
     CDS             complement(8291..8842)
                     /gene="coaC"
                     /locus_tag="ACHA52_001423"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8940150.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8839..9576)
                     /locus_tag="ACHA52_001424"
     CDS             complement(8839..9576)
                     /locus_tag="ACHA52_001424"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8940151.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9573..10562)
                     /locus_tag="ACHA52_001425"
     CDS             complement(9573..10562)
                     /locus_tag="ACHA52_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8940152.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10820..11362
                     /locus_tag="ACHA52_001426"
     CDS             10820..11362
                     /locus_tag="ACHA52_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8940153.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11379..12464
                     /locus_tag="ACHA52_001427"
     CDS             11379..12464
                     /locus_tag="ACHA52_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940154.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12476..13282
                     /locus_tag="ACHA52_001428"
     CDS             12476..13282
                     /locus_tag="ACHA52_001428"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940155.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13283..14110
                     /locus_tag="ACHA52_001429"
     CDS             13283..14110
                     /locus_tag="ACHA52_001429"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940156.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14107..15180
                     /locus_tag="ACHA52_001430"
     CDS             14107..15180
                     /locus_tag="ACHA52_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8940157.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15608..18304
                     /locus_tag="ACHA52_001431"
     CDS             15608..18304
                     /locus_tag="ACHA52_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8940158.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEEDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTEGLLATANNGYLFL
                     DEVHRLSKENQEKLFVFIDTGNYRPVGENVNWHSAKVRFIFATTEKSEDYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADILQKDIIVSKEALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTREEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNEKFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKYENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKEYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLKHILSSEMKPVEFNRLIQELIKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKAEEPNYDMKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18369..18869
                     /locus_tag="ACHA52_001432"
     CDS             18369..18869
                     /locus_tag="ACHA52_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8940159.1"
                     /translation="MIKPMFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVN
                     EEEDFF"
     gene            18881..19375
                     /locus_tag="ACHA52_001433"
     CDS             18881..19375
                     /locus_tag="ACHA52_001433"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8940160.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19397..20206
                     /locus_tag="ACHA52_001434"
     CDS             19397..20206
                     /locus_tag="ACHA52_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8940161.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20203..21024
                     /locus_tag="ACHA52_001435"
     CDS             20203..21024
                     /locus_tag="ACHA52_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8940162.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21075..23174
                     /locus_tag="ACHA52_001436"
     CDS             21075..23174
                     /locus_tag="ACHA52_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8940163.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23171..24289
                     /locus_tag="ACHA52_001437"
     CDS             23171..24289
                     /locus_tag="ACHA52_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8940164.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24328..25206
                     /locus_tag="ACHA52_001438"
     CDS             24328..25206
                     /locus_tag="ACHA52_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8940165.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25352..25549
                     /locus_tag="ACHA52_001439"
     CDS             25352..25549
                     /locus_tag="ACHA52_001439"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8940166.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8088 a         4448 c         5420 g         7616 t
ORIGIN      
        1 ttaacaaaat taaaaagtac ataagttatt attccgattt tcttgaaaca ataaaaaatc
       61 aaaaaaaata ctctcctaat agtccatctt cttttgattt tatttatata atgaaatcat
      121 cttatgaaaa ttactacttc atgtttagac aagagtaggg aggactaaga ataaatgtgt
      181 tcaaaaagac gttcaaaaca actaaataaa cacgcttgtc gtttagataa ggaacggtcg
      241 tgttctgaaa aacactacgt taagtttatg atgcgcacga tgctgatcag tttatctgta
      301 ggtgtctata tgcatgtacc cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc
      361 tctgtttctg agaccactga ttcccctatc actattactc atactttcac tgatgcaaat
      421 ggaaaacagc tacttcctgc attgacttcc tataataaag tcagttatcc tccaacgata
      481 aaccactact ggttaattcc taacaaaaca ttatggacat cggaaaccgg aagcagtgtg
      541 ccgctttcta tcttttccga tatgactgat cctacaaaag gtgatcagtt aaaacagatc
      601 actgatattt tgaatcatgc tttgacagat ataaaggacg gtggaagcgc acaagtttct
      661 tatgtatatg aaaaagatta ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg
      721 acaaaagttt cgatatctga tctggtagaa aggctgaaga acgcagatgg aacacaagga
      781 gatatgtcca aagtaatttc aaacaatggg aatatcgtgg atacgactaa tacaggaaca
      841 tctactattt ccttgtccta ttttgatcca attgctttaa aaacaatgac tgcatctgct
      901 gtgatccatg ttcgtgctga tcaaacagct atcaaaggaa aagatcttca cttaagaaca
      961 ggagagagtt acgcccctaa agatcttgtt gaatatgtaa caaaaagcga tggaacttca
     1021 ggaaataaag aagaaatcaa aatgaacggg acagtagata ctacaactcc gggagaatat
     1081 cccgttgtat taacttatat agactcgaaa acatcaaatg tggcagaaac aacagctgta
     1141 gtttatgtag aagcatctgc tgcttcatcg actgtcatca ctcattttat agatagtcaa
     1201 acaaataaag aacttcaagc agaaacacat atgagtagtg taaatcgtat tcctgatttg
     1261 cgcctacctg ctgaattttc tgatcgagtg ccaaatatag atttatcgac ttatgaacaa
     1321 aatgggaaag tcacttctat ttctgattgg atgcaagcaa acggattaga cagaaaaagt
     1381 cactggtcgg agatccttga agtacttcac tcagaactga tgaataatag gaagcaatca
     1441 ggaggggaaa ttacctttac ttatgtttat ataccagaca atcgttcatt agaaggtcat
     1501 gatcttattg ttcatccaaa cgaaaaaata aacaaagaag agttgatcca atcagcaaca
     1561 aattccttag gtaaacccgt accgttaaag gatgtatcgg ttacaatcaa tgaagtacca
     1621 ttggaggatg gttatttgac caaacaaaca ggtgatttta cgattatgta tagttatcat
     1681 gacccgtatt cactccaaga actccatgct cagtcactat tacatgtttt accattaaac
     1741 aaacagatag atcaaaaaga acaaaaaaga caagaagaaa gcttgtcaga aaaaaaacaa
     1801 caaatggctc atgcagtttc tccaacaggg aaagtaaaac aaacaagaag cctagagtta
     1861 gcaagaatat ctagaaaaat ctcagaatct aataaaacga taaggcaaga agctcatttg
     1921 gccccacaag tttctggatt ggaaaatgaa attttagagc ctaaaataaa acaacaatca
     1981 gaaaaagaat ttgttgctaa acagtcggaa gatcagaagg aatcagatga ccagaaggaa
     2041 acgaagaaaa aacgcttgtc aactattgat tccaccgatg agcatctgca gccgcaagta
     2101 ccacttccta gtccgggaac acccgcacct ggtggaggta ttccttcttc tgctttagga
     2161 gatttcatgc gaggtatggc aggaacatgg gtatacgcta atcgaaatga atttgacgca
     2221 gatgactggt tctgatttat atctctatct gttatagtca aataagtagc tgagtcatat
     2281 gttatttggc ataatggaga cgaggaggga tagaaaaatg ttagataaaa cgttacctta
     2341 cgcagaaatc tggatgacaa gaccacttca acaaaatgtt ccaatcacgc ctttagcgga
     2401 aggttttgaa tttcgatttt atcaaaaaga agatgaatct gcttgggcgg agattgagac
     2461 ttccgttggg gaatttcaaa cgacagatga agcacttgag tattttcaac aaaactttgc
     2521 tccttatcct gaagaacttg aaagaaggat gttttttgta gagacaaaaa aaggagaaaa
     2581 agtagcaacc tgtaccgctt ggcgaaaaaa atcaagagag caagtgtatc cggtttttca
     2641 ttggttagca gtgaaaccag attaccaagg aaaagggctc gcaaaagcat tgactgccag
     2701 agtcttgcaa gaatttccta ccctccatac ggacggacca atctatcttc acacacagac
     2761 atggagccat caggcgattg ctctttataa aaaaatgggt tttactttta ttgctgaaaa
     2821 tctagacggc acacctaatc cagattatga aaaagttatg caggttttaa gtaaattaga
     2881 taaataacga aatggtgaaa taagtcctca gtattgggat aaaaaacgaa atagtcattc
     2941 ctcatatctt acttttttga gttaatacta tcggataact aatggcatgc catacattca
     3001 aaaaagaagg ggcagtgaca aaagtattgt cactgcccct tctcttgttt tttagcatgt
     3061 tttatcgttt gattttttta ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt
     3121 ttcgactata gaggtaacca aatacactga aaacaactgc tccaataaaa ttaacaaata
     3181 aatccttcat agtatccatg attcccacgt ctaagtaacc attttggatg attgttttat
     3241 ctgttttacc atctgcattt tgactttcaa tgattgtccg agtgatatct ggaacatgat
     3301 agacagtgtt tccgtcttta cttaactcca cactattgat ttgctggatg atcgtatctt
     3361 tttgcatatc gaattgcagt aatttatctg ctgaaaattc cacaaactcc cacataacac
     3421 caactgtcat cgaaaaacag aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg
     3481 tttttgtatt tttattgagc aaataaacaa gggaaaagcc gacaccccca gctaaaaatc
     3541 cgctgaatgc atgtagaaaa gtatcccaca ttgaaaaata gccataaaaa tcacttaact
     3601 ctcctaaaac tgttgaacta aaaataaaag ataacaaaat gatttctagc atatttggaa
     3661 tctgtatctt taatatcttt tccagaaaat aagggatttc aaataaaatt aatgtcagta
     3721 ataaaagcat tgtatttccc cagtgttggt tccagacctg atccactagc ataataacga
     3781 cgatgatacg taataaaata tagattccgt ttgtgattct acctgcagtt tttgtactgt
     3841 tcgtaaaaaa ggttttcaaa tgattcataa aataactcct tcatgtatat ccttaattat
     3901 actaagaagc gtgtgtttac caaagctata tgacttaaag aaaaacagag ttgatattgt
     3961 gaaaaaaatc acttaatata gtataaggag aaaaggggga tacaaatgaa aaaattagtt
     4021 gctggtttat taacagccgt tgcacttctt gctatgagcg catgcggcgc agatcagaaa
     4081 aatacagcgc agacaagttc tagtacgaag gaatctgccg aagtatcatc aggcgcttct
     4141 gaacaagtat atacagatcc aaaagaaatg aaagaatctt atgatgtcgt gattattgga
     4201 gctggtggtg cagggatggc agcagctatc gaagcaaaag atgctgggtt gaatccagta
     4261 attttggaaa agatgcctgt tgccggagga aacacatcta aatcatcaag cggtatgaat
     4321 gcttcagaaa caaaatttca aaaagaagaa ggaatcaaag actcgaatga tgccttctat
     4381 gaagaaacgc taaaaggtgg tggaggaacg aatgataaag aaatgcttcg atactttgtc
     4441 gatcactctg ctgatgcggt cgattggcta gatcaaaacg gtatcaaact ggacaactta
     4501 acgatcactg gagggatgag tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt
     4561 ggtggatact tagtggaggg gttattgaat aacgtccatg agcgggaaat ccctattttt
     4621 gtcaacgcag atgtgacaga cattaagaaa aatggggaaa cggtcaatga agtgactgta
     4681 catgttcaag gagaagaacc aaaagaggtc accggaaaag caatcgtagt taccacaggc
     4741 ggttttggtg ccagcaaaga atttattgaa aaatatcgtc ccgatttagc tgattatatt
     4801 acgacaaatc aagaaggatc aactggagat ggtatcacca tgattgaaaa attaggtgga
     4861 caaaccgttg atatggataa aatacaaatt catccaactg tgcagcagga tgagggggtc
     4921 ttgattggtg aggttgttcg cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga
     4981 tttgtcaatg aaatggatac aagggataaa gtatctgcag caattacagc attgcctgaa
     5041 aaatcagctt atttgatttt tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt
     5101 tatgaccaaa aaggatatgt cacaactgga aagacaatgg aagaattagc agaaaaaatc
     5161 gatttaccaa aagaaacact agcaaaaaca gttgatggat ggaatacgga tgttgcggag
     5221 aaaaaagatg ctcaattcaa ccgtgccaca gccatggaac atgacttatc gtctccgaat
     5281 tattatgcga tcaaaatcgc accaggaatc cattacacga tgggcggtgt gaaaatcaat
     5341 acgaacactg aagtattaga taaagaaaac cagccaatta gggggttata tgctgctggt
     5401 gaagtcacag gtggcttaca tggaaacaac cggatcggtg gaaattcagt tgcggagatc
     5461 attatttttg ggcgacaagc gggcatacaa gcagctcgat ttgcttcgga aaatgaatga
     5521 aatagaacgt aaataaaaag aggttgtgaa atcagcgttt tgagctaata ccgagggtca
     5581 gctgattgaa cactgtttag tgaagaggtt gtgaaaaagc tgccctgcat caagtaataa
     5641 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga
     5701 tgcaagcttt tgaacaccgt ttattcggaa aaagagcgtg ggacaaaagt aaagaacact
     5761 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagaaattcc ttcggaaata
     5821 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
     5881 ttaaacgctt ctgtcccagc ctcttttttg atcagcatga ttttattcag ttatcttcaa
     5941 aatgattttt ccgatgtttt tgtttgcttc catatattca tgcgcttgtt tgactgcgct
     6001 gaattggaat gtacggtcaa tgattggccg aatcttgcca ttttctagaa aaggcccagc
     6061 tttttgcata aaagtttgtg cgagctctgc tttatactga tcacttctag gtgtcaataa
     6121 tgtaccaagc agttggacac gtttcccaat caatacacca aggtctatct caggcacgat
     6181 ggtcccacct agcataccaa tcaagaccca acgtccatca acggctatag aagctaaatt
     6241 cttttcccag taagaagcgc cgatgaaatc caaaataaca tcgactcccc gaccttcagt
     6301 gatttttcgt acctcttctt gaaagtcttg ttttttatag ttgatcaatt catctgcacc
     6361 aagttcttta caaaaggcga gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg
     6421 agaaagctgt ctagctaatt ggatcgctgc tgttcccaca ccacttgctc ctgcatggat
     6481 caagacagtt tcgccttctt gtaattttcc aagccagtaa agtgtttgat aagcagtcaa
     6541 aaaaacttct gagattccag cagcagaaac atagtctagc gtatcaggga gaaggattgc
     6601 acgatcggca ggcatcacag catattcggc ataaccacct agattgacta atccgtatac
     6661 ttttgtccca ggagtaaaag tggagatatc acttttattc tcaactacct ctccagctac
     6721 ttcgactccc aaaatagggt agggaggttt caaagagact gattctcttt tcataatatc
     6781 tgttcgattg acagctgctg tatggacttt gatcagtaat tcgccttgtt ttggtttagg
     6841 aacaggcact tcaatttgtt ctaatacatc tacagaacca ggatgcttga ttgaaatggc
     6901 acgcatggat ctattcctcc tttgcccatt cttggaatgc tgtattcaac tctttgctac
     6961 cttcagaaag tactagagtc gtacgagaaa tatccgtttc ttccactaga gcggcaagta
     7021 cagcagcaac tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt
     7081 gaatggaagt aacttcttca tcgttagtca atgtgactgg ttgaagaatg acaaaatcta
     7141 agttagttcg tttcagccat tcatctgcat aatgtttagt aatataataa tctgtcatgg
     7201 actcaggcca ttttgctggt tcatctgcat aaacagcact gaccatcaga tagcgagaga
     7261 catgcgcatt ttcagctgcg atcactgttt tgattgcgcc atccaagtct acttgcagaa
     7321 gattctttcc ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg
     7381 tcattttttc cacggaccaa gttaaatcaa aagagacata cgaaacattc tcttttttta
     7441 ctaatgtttg ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta
     7501 aatcttttat caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa attttcattt
     7561 ggatcatcct ttcgacgtca aatctttcca ttcaatagta acaccaaaag agaaaaaaat
     7621 cagagatcgg ctacgatgac tatcagttag taagagaagg gtgcttgaag tatcttaaca
     7681 agacattgca tactgctgca gtcgcaagac ctgcgacgat tccttcaggt ataccacttg
     7741 taaaaataac cgctagaata gctgaataaa cagcatttaa atttgttccg atgactttgg
     7801 cataatcttg ctggaataaa aagtagatca tattcatcac taaaatcgta ttggtcaatg
     7861 aaccggaaag tccagctaag aataaagaaa cggaatctgc tttttgtttc attagtttgc
     7921 gcagaccttt ataaacgaaa taaggaacca ccccaatcaa gatgcgagga ataaacgcga
     7981 tcagcaaggc tttccagctt ccatgttctg ttccgatgac tgggataaac ggagaaaaga
     8041 caaaagacaa aggtgtttgg ataactgtgc tacggatcat gctgatcagt ccaaatgtac
     8101 cgccaagaaa agcacctagt cttggtccta aaacgatcga agcaatgatt acaggaatat
     8161 gcatggttgt tgcattgatt ggaccgatag ggataaaacc taaaggcgta acagctaata
     8221 aaatcaaaat agctaaaaac atcgcagtta atgtaaagtt ttttgtattt ttcataagta
     8281 ctcctttttt ctaagcattt ctttcattaa gagtcagcat gacctgttcg atgatcgtct
     8341 gaatatcagc taaggcacct tttccaaaat ctccacaagc taaaagagat tctcttggtt
     8401 caatttcctg atagccgaca gatctcaatg tttccagatt acgctgagtg atcgggtttt
     8461 gatacatatt cgtgttcatg gcaggggcaa tcaattttgg tgttcccact ggaagcgcca
     8521 ttgcgactgt tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg
     8581 ctgtagcagg cgcgatgaga aacaagtcag cttgttttgc tagttcgata tgattgatta
     8641 cctgcggatt tgattcttgc atgacgtctg tatggactgc gcgcttcgac agagattgca
     8701 aggttaatgg cgtgataaaa tgtgtgctgc ttttagacat aatcacatct acctgggcac
     8761 ctaatttgac gaattgactt gtaagatcag cgcttttgta agcagaaata cttccagtaa
     8821 cgcctagtaa gatctttttc attttgattc ctccagtcta attttctcag taatcaatgc
     8881 agcaatctca gatttacttt gagcttcttc cactgttcca tctttagata agagatagcc
     8941 gtggtgtgtc tcgccatgga tttccgtcaa atcatttgcg aaaataaaat cagttttgtt
     9001 atttatcaga ctattttttg cgactgccag aagagattcc tttggtacat ctactaatag
     9061 tttgaaacca atgatttttg cttttggctg ccattcacgt agataagaaa tgatttttgg
     9121 tgttttcttc aaaacgagca caagatgctc ggtgttagaa gagatctttg aaacagtggt
     9181 atctgtctgt tcattttccg caaaccaatt gtccagttct tcttgcgttg aagaagtggg
     9241 taggtttttt gccagctgtt cttctgaaaa actaaaagca ggagtaaaat cactgactgc
     9301 catactgtgg ataatcgcat cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa
     9361 taagtccagt gcagattcga tcgtataaat cgttatattg gagtgtcttt tcggtttcag
     9421 tgcttgtttc gtagtcacgt aatcgattgt ggtatccgga ttggcagcta aataatcagc
     9481 taatgcttgc cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt gatcgatttt
     9541 ttcagtcgtt cctccagctg tgattagaat cttcatttat ggtcacctac atttactagt
     9601 gttcttcctt ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa ttgatccaga
     9661 gtaatctctt ggacgaccag ttcatctgca atctgcaaat ccgccatctg ttgccataaa
     9721 gaaagacgtt gatctattgg tacattgaca gaatcaattc caatcaactg gatgtttcgc
     9781 agaataaaag gcaagacagt cgcgtttagt ttgattcccc cagcattgcc acataaagct
     9841 actgcaccgt tataagagat caatggaaga agactgctta gctgctcgcc acctactgtg
     9901 tcgatgacat agtcgatttg ctgttttcct aaagcttttg ttgtctctgg cagaaattca
     9961 tcaggtcgta cgacttgcaa agcacctaga ctttttagcc atgtgacaga ctcttctttt
    10021 ctggaaaagg caatgattga ctggaatcct agtttatgta acagggctat cgctgtactg
    10081 cctacaccgc cagaagcacc agtaacgaca acttttttat cggaggtcat accttgacgg
    10141 attaaagcgt tcacacacaa catagcagta aaccctgctg taccaaggat catcgcttga
    10201 cgcagcgtca tattttttgg tagaggaact aaccagtctc ctggtacttt ttgatattgg
    10261 ctataaccgc ctggatgggt gacgccaagc ccaaaaccag tagcaagtac aagatcttct
    10321 tttgaaaaac gtggattgtt actttggacg acagttccgg ataaatcaat ccctggagtc
    10381 atcggatatg ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc tttataattg
    10441 acatcagaat aggcaatttt taccaagact tcattttcag ccaactcagt aaatggcaga
    10501 tcactgattg ctgtatggaa aacaggttcc tttattacct gaaaagatcg aaaagcgttc
    10561 attcgtgaga cactcctttt aaaattttct tttatcatac cataaagcct actcttttat
    10621 tcttgttctt ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc aaacaactag
    10681 aatttattcc aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt
    10741 ttcaataaat ttcgtatact aatacgagtg gtagtaaaca ggaagtttac tattagaaaa
    10801 cttcgattcg gaggaaatta tggaaattgg tgagaagtta agaaatttgc gcatccaaaa
    10861 gaatttgaca caagaagaac taggcgaacg cacagatttg tcaaaagggt atatttccca
    10921 gttggaacga gatttaagtt caccatcaat ggaaacattc ttttctattc ttgaagtctt
    10981 aggtgtgact ccagaagaat tttttcatca agaagcagcg aatctgcaag tggtttattc
    11041 aaaagaagat catacggttt atttcgatga agaaaacggg tatgaattgg aatggctcgt
    11101 ggctgattcg aatgaaaaag aaatggagcc tgtcttgctg acttttgaag gtgcaggaga
    11161 atataagacg tttgaacctt cattatctga aacgtttatt tttgtattag aaggggaatt
    11221 agagttaagc ttaggagaga cggtctatgt ggcaaaagca ggacagtcga tctattatca
    11281 agcgacaaaa caacatcaat taaggaatca tctcaaaaaa cgaacaaaag tattgattgt
    11341 tgcaacgcaa tcatatttgt aatatagggg gtaaaaacgt ggggaaacca atcatttcat
    11401 ttgatcaagt agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta agttttgaga
    11461 tcgaacaagg aaaattttat acactgcttg gtccttccgg ctgtggtaaa acaacgatcc
    11521 tacgtattat agctggattc accgaagcta cagaagggga catctatttt gaagggaaac
    11581 ggttgaatga tttgccagct aataagcgac aagtaaatac agtcttccaa gattatgcac
    11641 tttttcctca tatgaatgtt tttgataacg ttgcttttgg attgaagatc aagaaaatgt
    11701 ccaaatcaga tatcgaaaag aaggtaaaag atgctttgcg tatggtccag cttcctggat
    11761 acgaaaaacg ggagattagt gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac
    11821 gagcaattgt aaatgaaccg aaagtattgc tattagatga accgttatcg gcgcttgatt
    11881 tgaaactacg tacagatatg cagtatgaac tgcgggatct gcaacaacga ttaggaatca
    11941 cgtttatttt tgttactcat gaccaagaag aagcgttggc aatgagtgat gagatttttg
    12001 tcatgaataa agggaaaatc gtccaaagtg gtacacccgt agatatttat gatgagccaa
    12061 tcaatcattt cgttgctgat tttgttgggg agagcaatat cgtggacggt gtaatgatcg
    12121 aagataatct agttgagttt gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc
    12181 taaatgaacc agttgaagtt gttttacgcc cagaggattt gacgatcacg acaccagata
    12241 aaggaaaact agtcgtaacg gtcgatactc agctgtttcg tggtgtccat tatgaaatca
    12301 tctgttatga tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa gctaaagaag
    12361 gaagcaaagt aggattggcc tttgagccag aagatatcca tgtcatgcgt ttcaacgagt
    12421 ctgaagaaga attcgatgca cgtcttgaca gctacgaaga atagggggga caagaatgaa
    12481 aacaatgcgc aggatctatt ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc
    12541 gcctgtatta atgatcattt atcaatcttt ttttgatatg aatgggcaat tcacgctgaa
    12601 taattatcta tcttatttta cgtcggggac atcgctttct atgacgatca attctgtatg
    12661 gtatgcattt ttgatcacgt ttttcacgct gatcatcagt tacccgactg cttatttttt
    12721 aacaaaactg aagcacaagc aattatggct gatgctgatt attttgccta catgggtgaa
    12781 tctgctgctt aaagcttacg catttattgg gattttcagt gtgcacggca gcgtgaatca
    12841 tttctttgat tttatcggga tcgggccgaa acagatatta ttcacagact tcagttttct
    12901 gttcgtggca acgtatatcg agattccatt tatgattatg ccaattttca atgcattgga
    12961 agagatgaat ccttctttga tcagtgctag tagagattta ggcgcgaata gctttgagac
    13021 cttccgacga gtgatttttc cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt
    13081 atttattcct tctctttctt tattcatgct gactcgtctg attggcggaa atcgggtgat
    13141 cactttagga acggctatcg aacagcattt cttggttaca caaaactggg ggatgggttc
    13201 tacaatcggt gtgattttaa tcattgctat gttcatcgtg atgctcctga caggcgagaa
    13261 gaaaaaagga ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt atttagggat
    13321 cgtcttttta ttcttatatg caccgatctt ctatctgatt ttttattcct tcaatgaagg
    13381 aggaacgatg aacagcttta caggatttac ttgggaaaat tatgaagctg tttttgaaga
    13441 tacacgtttg atcattatcg tattgaatac gttcatgctg gctttcttat ctgcattgat
    13501 cgccacgatc atcgggacat ttggggctat gggaatctat tacacgaaaa aacgacgtgc
    13561 acggacagcg ttgttgagca tgaataacat cttattagtc tctcctgatg ttattatcgg
    13621 tgctagtttc ttgattttct ttacactact aggattcgga ttaggttttc agagtgtatt
    13681 gctttcccat attgctttca gcattccaat cgtcgtcttg atggtcttgc caaaattgca
    13741 ggaaatgaac gattcaatgg tcgatgcggc tagagacctt ggtgctaata atttccaagt
    13801 agtcaaaaat atcattttgc cttatctttc tccaggaatc atagctggat attttatggc
    13861 atttacctat tctcttgatg attttgcggt tacatttttc gtcacaggaa atggctttac
    13921 gacgctctca gtagagatct attctcgtgc aagacaagga atcagtttag aaatcaatgc
    13981 gctaagtgcg ttagttttct tgttctccat gatattggtc attggatact actttattag
    14041 taaagaaaac cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg
    14101 gctgcgatga aaaaattaca atctctgttt ttaggtattc tcatcattat tgcggtgctg
    14161 ctgcttggtg ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc
    14221 atctataact ggggagatta tatcgatcct agtttgatca cgaaatttga aaaagaatat
    14281 ggatacaaag tgaattatga aacatttgat tctaatgaag cgatgttcac aaaaatccag
    14341 caaggtggga caaactacga catcgctata ccaagtgaat atatgatcca aaaaatgatc
    14401 aaagaaaaac tagtacttcc attagatcat tcgaagatca aaggattaaa taatattgat
    14461 ccacgatttt tagacttgga ttttgatcca gataatcagt attcgattcc atatttttgg
    14521 ggaactttag ggatcgtata taacgataaa ttctttgatg caaatcaaat taagcattgg
    14581 gatgatttgt ggaagccgga attaaaagac agtttgatgc tgattgacgg tgcacgagaa
    14641 gttatggggc tttctttgaa tagtttaggc tattctttga atagtaagaa tatggcacag
    14701 ctgacagaag cagcaaataa attgaatcat ttaacaccaa atgtcaaagc aatcgtggca
    14761 gatgagatca aaatgtatat gatcaatgaa gaagcctccg tggcagtgac attttctggc
    14821 gaagctgccg atatgatgtg ggaaaatgaa catttacatt atgtgatccc accagaagga
    14881 tccaatcttt ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc
    14941 tatgatttca tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt
    15001 ggttattcta caccaaatga agaagcaaaa gcgttattgc caaaagacat ttcgtcggac
    15061 gaacaattct atccatctga tgacacgatc tcccatcttg aagtctacaa agatctagga
    15121 tcgaaatacc taggcatcta taatgaccta ttcttagaat tcaagatgta taggagataa
    15181 tggcaaaact cagtaagaat ggggacttat caacgttcta aagagctaaa aatcgtcctt
    15241 tttctatctg agttttccaa tcttctacca aaagcggatg aaacgccgct ttttttgtgt
    15301 tattttattt tttctaccac gtttttctac cagagaaaag tggacaaaac cattgtaaaa
    15361 tagggcccga gacgggctag taaatctaag attgaaaaag ttacagaagg ataaagtaat
    15421 tggaaactat gcaaatatac ttaacccttc tgtaatctat taataaaact aatatatgaa
    15481 tccagttcaa tatttgtagc tatgaaaaaa cataaaaatc atattagata aaaactaaat
    15541 tggtagataa attattttgt cagatatctg aggcaaatat tcgactattt gtgtttcaaa
    15601 gtaaaaattg gaatacaaat atagaataga catattgcag ccgatagttt attatgcttt
    15661 tagacacatt aaactggagg aaaaaatgaa aaagagcgct aaagatcgag tttttgatga
    15721 gctgatacga ctaacaggga atatgtcttc tgtcactgcc gagaaaattt cgaagaatct
    15781 aggtatctct cgtcagaatg ccagccatta tttgacgaga ttagtggaag ataaaaaggt
    15841 cgagaaatta ccgggaaagc ctgttttgtg gaagccttta gatgaatatg cggttgtaga
    15901 taatacggag caaataaacg aagcctttca ttctgtggtg ggtcatgacg gaagtctgag
    15961 agaggtcatt caaaaatgta ttgcagctgt caaatatccg cctaacggac tcagtgtatt
    16021 aataaatgga gccacggggg ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc
    16081 tatccacgag caaattattg aagaagatgc accatttgct attttaaatt gtgccgatta
    16141 tgcggacaat cccgaactgc tttcggcaac actgtttgga tacaagaaag gggcctttac
    16201 gggggctgag aaggatacag aggggttatt ggcaacagcc aacaatggct atctttttct
    16261 tgatgaggtc catcgtttgt cgaaagaaaa tcaagaaaag ctctttgttt ttattgatac
    16321 tggaaattat cgtccagtcg gtgaaaatgt aaactggcat tctgctaagg ttcgttttat
    16381 cttcgccacg acggaaaaaa gtgaagatta tttattagac acctttgatc gacgtattca
    16441 aatcagtgtt atgttgccaa cttttgaaga tcgtcccatt cgagaacggt tagagctgat
    16501 tcaattattt tttcaaaatg aagcggatat tttacaaaaa gatattattg tttcaaaaga
    16561 agctttgtct ctaatgttat ctcatccctt tagtgggaat attggtaagc tgaagaatat
    16621 cattaaaatc agttgtgcgg atgtgtacag tcgaacacgg gaagagccat taacgattgg
    16681 gaaaaatgag atcttgattc agttaaatat gctggaatcc gaagtagaaa gcttgccgat
    16741 tgctagttta ttgataaaga aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga
    16801 ttatgattcg attttggaaa aaattactca gctgataaat gaaggtaatt tcaatgaaaa
    16861 gttttcttcg ataaagaatg aagtacagca tttggtgcat cacattgata aggattcttt
    16921 tttatcaatg agtagaaaat tgatgattca gatgtttaga aaggtctggg tatccgtttt
    16981 aggaaaaacg tacggattga cgacgagtgg acctgttgca gaaattatta gcagatccta
    17041 tgtcatttcc ccaagctata agacagactt aaccgggata aatcgtacat tagctgcaaa
    17101 tctacctcgg acgacttact tatgtaatca gttcttggag catttaccac cgctctcaga
    17161 aaagtacgag aactttctaa gactgcttat tgtcgtgact ttatctgatt atgtagatga
    17221 atccatcgag ctgaagggcc tgttgcttgc tcacggagat agtacggcta gcagcattca
    17281 agcagtagtg aatcaattat gcggcaatta tgtttttgaa gcgttggata tgccagtaga
    17341 gagtaatgtg tcagagattg tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac
    17401 agatagtcta gtgctgatcg tagatatggg ctctttaaat caaatctaca cacagatcaa
    17461 gaacgattta aaaggagaat tacttcttct gaataatttg acgacctcca ttgcacttga
    17521 tattggcctg aagatgacga tgaatgcgcc atttaaacag attgcggagg atgcagcaga
    17581 caagtattca atcaatgttc aatactttga aggcttttct caaagtagca acatcatcat
    17641 ttcctgcatg tcgggattgg gtatctcgga taaactgaag gatgtttttc gtcatcattt
    17701 atcggatgag tccatcgaag ttttcacgaa ggaatacaga gaactacgtg aattaatcga
    17761 ccaaaatgat gaaacttatt ttgacaagac aaaattggtc atcacgactt cggatcttcc
    17821 acgttccttt tcaattccta atatcaatgt atatgatatt ttagatggtg atggagcagc
    17881 caatttgaag catattttga gttctgagat gaagccggta gaatttaacc gtttgattca
    17941 ggagctgata aaattctttt cattggaagg aatcgcagac cgtttgaact ttctgaaccc
    18001 aacaatcgtg atgagtgagg tggaaacagt catttctaaa tatgaaaatt actatcattt
    18061 cactttaaat ggccgcgtaa aactaaatct ttatatgcat acggcattga tgatggaacg
    18121 tttattcttg gcgcggacag aaaaagcaga agaaccaaat tatgatatga aaccagatga
    18181 agatgaattt tattatgttt cgagaagtat ttttcaatca atggagatga aatataattt
    18241 taaagtcaat gcttatgagc tttcgctgct ctatgaatta ttaggaccgt atattcaaaa
    18301 ataaagtgtc taaaaaagtg tctaatctga ttttttcagg ttagacactt ttttttatgt
    18361 gaattaacgt gattaaacca atgtttttaa aagttggcac aggaattgct tatatgaaag
    18421 tgaacagaaa gaaaggaatt aaccatatga agaaaattct aatagcgact cacggtcact
    18481 tagcttcagg ctttcttagc agtattcaat tactgacggg gaaggttcaa gagattacgg
    18541 tgattaatgc ttatgtggat gagtgtgatt atgaacagga gttgaatgta tttgtaaacc
    18601 aggtgaacga gcaggatcag atttttgttt ttaccgattt atttggcgga agtgtcaatc
    18661 agaagattac caaaaccttt ttggagaaag agattgctgt aactattttg tctggcttta
    18721 atctgccaat tctgctcgaa atagtattgg caaccaagga attaacaaag gaggatattc
    18781 aggaactggt aaataaatgc caacaggaaa taaagatgac cattcttgat gaggtacatg
    18841 tagtaaatga agaggaagat tttttttagg aggaagaaaa atgattaaag cagtgcgaat
    18901 tgatgaacga ttaattcatg gacaagtagc gatgacttgg acaaagagtc taaatttaac
    18961 cgggttagta gtagccagcg atgaggcggc taccaacgat attcaaaaga tgacattgaa
    19021 gatggctgca ccatcgaatg tcaaagtcat tattaaaaca gttgatgacg cgattcattt
    19081 gttaaaagat ccgcgggcga aggacatgcg cttgatggtt ttagtcccta atgtcaaaga
    19141 tgcggtaaaa gttgcgaaag ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc
    19201 tgggaaaatg acaccgccat cggaagataa gaaaacctta tcaaaggaag tgatgttaac
    19261 gacgtcagaa attcaagact taaaagaatt agttgaactt tatccagata ccgtttttca
    19321 gcctacaccg gcgatggaaa aacgttcagc tatctctatt ttgagtgatt tttagaaaaa
    19381 aggaaggaga aaaattatgt atgatgcatt tgtagtcgct ttggccgttt ttattggagt
    19441 tgctggacat gaaatgttcg ggatgtccat gctgagtcgt ccgattgttg ttgctccgtt
    19501 agcaggagcg ttgatggggg atttgcagac ggggttgatt gtcggggcgt cactagaagc
    19561 catttttatg ggtgtcgtca atatcggaat ctctagtacg gcggagcctt ctttggctgc
    19621 tgggttggct acagcgttcg caatcaaaat gggaggaaac gtggatgcga ttattcccat
    19681 tgtctttcca ctagcagtat taggattaca gttaatgaat atgatttttt cttttgtatg
    19741 tggaccgatg gctgctaaat ttttcaaatt tgcaaaatct ggtgatcaaa aaggaatcat
    19801 caatctgcac ttcgttatgt ggtttgtcca ctatgcgctt tatgctctta ttccattctt
    19861 tgccgtttta tttggagcag atgttgtcaa aagcgtcttg gataatattc cagaggtcat
    19921 catgaacgga ttaacagtcg ctggaaatct gctgccggca gttgggatgg ctatgctgtt
    19981 acggatgctt tggagtaaaa atattgctgt gtggtttttc tttggggccg ttgttatggc
    20041 gtatctcgag ttaccattga ttgcaattgc cgcagttgga acgattattg caattgttat
    20101 agcccaaaga gatctacaac taaaaaatat atctgttcat gcagttcaaa gcgatgctgc
    20161 tgtaaatgca acgcttgacc aagaagagga ggatttcttt caatgaaatt agctgatgga
    20221 atttctaaag atgagaaaaa attggttcgg aagatgtttt ggcgctcgat gactatgtat
    20281 gcttcggtga atccggtgac gatgggcggc ggtggtttcg cctattctat gatgcccttc
    20341 attaatcatt tctacaaaag tgaagaagat cgagctgcgg cgctggaacg tcatacacag
    20401 tatttttcga cgaccattcc ttgcgcgtcc tttgtcatgg ggattgcggc atcgatggag
    20461 aaggaaaatt ctgaaaaagc tggttttgac gtggaaagta tcaacgcgat caaaacgagt
    20521 ttgatgggac cattggctgg aattggcgat tcattgttct ggggagtttg gcgtgtcgtt
    20581 accgcgggac tagcgatcaa cttagccaat gccggaaata ttttggcacc aattatcttt
    20641 ttagttatgt tcaatatacc taattatttg atgcgatact atggcggcgt tttaggctat
    20701 tcattaggct ctaaatatat tgaaaagctg tatgcgaacg ggttaattga aattctgaca
    20761 aaagctgcta gtatcttggg tctgatcatg gtaggcgcaa tgacgatcag tatggtcaaa
    20821 ttcaataccg ttttgagctt ttcaatgaag ggcgaagaac taatgaatgt ccaagaaatc
    20881 ctcgatagta tttttaaagg aattgtaccg ttattggtaa cattgggctg tttcaaatta
    20941 ctagataaaa aagtcggcgt catcaaaatt atttttggaa tggtcgtctt gggaatttta
    21001 ttgtcattac ttggaatcgc atagtaggaa gctgcagcta ctaggctgca gtttttttag
    21061 caaggaggga aaagatgact attaatgaag taatttattc acccaaacac ccattattta
    21121 atgctgagtt aaacgacgga aatgtgacta cttcaaacga atacagctac tatcggaata
    21181 ttcctgatgt aaagggattg accttcgatg taagtaatca aaagacatta gcaaaccttg
    21241 atgtttgcgg gacgattcaa acgttgacct tttatcgaga aaatttacta actgaggaaa
    21301 aaccaggtgt ttgggtcaat aaacagctct ctcaaaccaa ttcgttatcc ttaaaggttg
    21361 aagtaaacgg gattgtctac catttgtcag aatcggatca tcgtattgaa gtcgatgtga
    21421 ttcaagactg tttgcctaga tatattcatc attattcgac gtttaaggtc attgtagtat
    21481 cctttgctcc catactggct aataagcgtt taagtatgct tgttcaacag gtttacattg
    21541 tgaatgaaac acacgaaagc cttcaagttt cagtgaaaga tgtgccttcg tatcaagaga
    21601 agtattcgga tcagcaaaat gtgttgattt ctcaaaaagg aaataaggac acgatcgcac
    21661 aacaggaagt ggcaagcttc gccattgctt ttgtggaccc aaatgctttt gaagaagtaa
    21721 tgacttttca ggaatctgat atcgaaaaat ggctgaagga aacattgaac tattttgctc
    21781 agctatttgg gcagctgtca atgccaaacg atagagttgt ccatctctat cgtcgtgcac
    21841 tttatcaaag cttttctagt ttcggtatga atcgacaggg acaggtcgtc ggcagcaatt
    21901 ggggcagcta tcctgctact aatcgaattt ggaataagga tatgtattac gcatcattgc
    21961 cattcaccat gtttgaaccg gagttatgtc aaaaaacggt tctatggttt gatcaatatg
    22021 gcgttaagtt tcctggttca aaatttacag gaggtatcaa tcattctctg agtaattcgt
    22081 tatccagtgc gttactggct agtctttatt ttgaacatac tggggataca aaattctttg
    22141 agcggtgtcc gcaggttcta ctgaatgctt caagaattat tgaggacata ttagctcaac
    22201 gtcggtcagg ggaaccaatg ctttttccat ctgtttggtt atcagacgcc tttgcacttg
    22261 gaaagtatca taccggcagc aatctttgtc tttggaaggc ctgtattgga cttgcagaaa
    22321 tttttgaagc cctagaacaa ctgagtctta gcaagcgata caaaaacatt gcctgtaaac
    22381 taaaggaatc aatttgtaaa caaatgacta tcgatggtcc ttttggtgag caatttttgg
    22441 agggaatcgg tgatgaggag aaaacaacct attcagtgaa gcactatcaa aaaccgattc
    22501 ttgagcaagg gttaatcttt ttaagtgatg tgattgtgga tggaaaaatt aatttattga
    22561 tgcatgatgg agaagaatca gacactacgt tgatgccttt ttatcaattt ttagataata
    22621 aagatcattt atatcagaac accatggcct tttctgctag ttctttcaat ccaacctata
    22681 gcgaggaaat caaaggcatc acttggggat tagaatccgg cgccactttt cctggcttta
    22741 tcactgtttt aatgagcgag ctgcacaacc ctcaagcttt cgtaacccga ttagaagaac
    22801 taatttgttt ggcagattta gacggttcgt ggtggtggtg gccgtatcgc ttagaggctc
    22861 aacaaggaga agtcgtgcga gactttggct gcggcaaatg cggctgggca tcgggtcttt
    22921 ttgtcagcct aatgatcagt caatatttcg gattgaaatt caaaaaagga gtattgcaaa
    22981 ttgatcctgt tgataattta acgttttctt ggaagaattt gaagttcggt caagccttca
    23041 tttcgattga atgtacgcaa tccgagttga gtatttcaaa tttgtcagaa aagccattga
    23101 aaataagtga tacgaaaaaa attcttcacg tagaaaaggg aaaaacgatt cacattcaaa
    23161 ggagaaaaag atgagtatac taaaaaatat tacccaattt aaaaaaatcc agagtcgagc
    23221 aatcactccc gaaaatccaa caggcgagaa aggtaaagct gcgatgttgc caagcgagct
    23281 aggaccaagc cgcaaaggtc gtggcgcaat tccgctgact tctggtgaag aaactgtgat
    23341 tgcggatatt actggaacgg gaataattcg tcatatgtgg atgacgattc gtgatcgaac
    23401 agaatttggc agttttgttt tacgtgattt aatcttaaga atttattggg atgggtcgga
    23461 tagccctgct gttgaaagtc ctttagggga tttcttttgt aatgggtttg gagagcgttg
    23521 cgaggtgaac tcgttgccga tagtggttaa tcctacaggg ggctttaatt cctatttcga
    23581 aatgcctttc caaacatccg ctaaaattac gatcgagaat ttacatccga aggatatgtc
    23641 ctcatttttt tatacgataa attacgcttt ggtggacgaa ttggaagaaa atagcctata
    23701 ttttcacgca aattggcatc gggaacgcat taccaaatta caggaggatt ataagttaat
    23761 tgatcgatta aagggtcaag gctattacgt gggtacttac cttgcgctta cctgcctaga
    23821 acgttattgg tggggcgaag gcgagtttaa attctacatt gatggggata gtgagtatcc
    23881 gaccgtaacc tcaacaggtt cagaagatta ttttggcggg gcgtgggcct ttcatgagta
    23941 cgatgagttc ggacgtccac atgcgaaaaa ctattcaaca gcctttttag ggtatcctta
    24001 ccaatcaaat agagatgcca caagagatcg ttttgaaacg ggaaaattga atgccgttca
    24061 tgcctttggt gacgacgcaa tgccacgaca tggtctttat cgttggcatc ttcaagaccc
    24121 catctgtttt accgaagatt tatcggtcac tttacaacaa atcggcaacg atgatgttcg
    24181 cttatttgag cggcaagacg atgttgctag tgtcgcttac tggtattcaa cagaagcttc
    24241 aggaaatact aaacgtcatt tcactgatcg agaacgcttg ccgcgttaat gaagtgtttt
    24301 tcacatattc gaagggagga acctatgatg atatatgggg caattgaagc tggagggact
    24361 aaatttgtct gtgcagtcgg tactgaggaa ttaacgattt tggaaagagt cagttttccc
    24421 acaacaactc cagttgaaac aatgaagcag gtcattgatt tttttaaacc ttttaaggaa
    24481 caattggcag gcatcggggt cggctgcttt ggtccaattg atattcaatg caaatcaacg
    24541 agtttcggtc atatcacatc aacaccaaaa cttgcttggc aaaatttcaa ttttgtagga
    24601 acgctaaagc aatatttcaa tattccgatt gaatggacaa cagatgtcaa tgcggcttgc
    24661 tatggtgaat atgttgctgg ttctggcaaa gggttagcag gtgttgcata cttcactgtg
    24721 ggaacaggta tcggaggtgg tgccttgttc aatgggactt tcgtggaagg gttcagtcat
    24781 ccggaaatgg ggcatatgtt gatcaagcga catccaaagg acaatttttc aggaaactgt
    24841 cccttccacc aagattgttt agaggggatg gcagcaggac cagcgattga aaaacggttg
    24901 ggtgtaaaag gccagaattt attggctgac gattcgtttt ggcagattga agccttttac
    24961 cttgcccaat gtgcatacaa tacaacactg atgttttcgc cagatagaat tatttttggc
    25021 ggtggtgtaa tgaaacaaga acacatgaag aaaaaagttc aggataaatt tgttgagctt
    25081 ataaatggat atgttgaaat accaccaatt gatagctaca ttattacgcc cgaattaggt
    25141 gataatgccg gaataatcgg cggattagca ttggccagaa aagcggtaag aaataagcaa
    25201 ccataattaa tgaatgtttc attgatactc atgtcaaaag tatttaaccg agtaatcaaa
    25261 tgatcaaatg aagttggata aagcgaaaat gaattcaaat aaaaaggctc taagtcaaat
    25321 cgtgttgttt tcattaaatc actgaaagga aatgcttctg cgtttccttt tttctttttg
    25381 ttgtgcgctg tataccttga ggatattcac tggtggcgta cacttaaata gtaatatatt
    25441 gtgttacttt atgtctgtgg taatagttat agttgcttac caatgtatgt tcatttttcg
    25501 aaatgttgac ttcataatcc ttggcttaat gtttgaattt ttttggtgat agattttttc
    25561 agccgtataa aa
//
LOCUS       DAYYGY010000036        25496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000036 DAYYGY010000000
VERSION     DAYYGY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.037"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHA52_001440"
     CDS             127..3654
                     /locus_tag="ACHA52_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8940167.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHA52_001441"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHA52_001441"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8940168.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHA52_001442"
     CDS             7442..7783
                     /locus_tag="ACHA52_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8940169.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHA52_001443"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHA52_001443"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8940170.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHA52_001444"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHA52_001444"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8940171.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHA52_001445"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHA52_001445"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8940172.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACHA52_001446"
     CDS             12994..13731
                     /locus_tag="ACHA52_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8940173.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACHA52_001447"
     CDS             13745..14560
                     /locus_tag="ACHA52_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8940174.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACHA52_001448"
     CDS             14573..15220
                     /locus_tag="ACHA52_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8940175.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACHA52_001449"
     CDS             15236..15892
                     /locus_tag="ACHA52_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8940176.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACHA52_001450"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACHA52_001450"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8940177.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACHA52_001451"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACHA52_001451"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8940178.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACHA52_001452"
     CDS             18234..18752
                     /locus_tag="ACHA52_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8940179.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHA52_001453"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHA52_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8940180.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="ACHA52_001454"
     CDS             complement(19639..20559)
                     /locus_tag="ACHA52_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8940181.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20685..21737
                     /locus_tag="ACHA52_001455"
     CDS             20685..21737
                     /locus_tag="ACHA52_001455"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8940182.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACHA52_001456"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACHA52_001456"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8940183.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="ACHA52_001457"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="ACHA52_001457"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8940184.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="ACHA52_001458"
     CDS             complement(23377..24138)
                     /locus_tag="ACHA52_001458"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8940185.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="ACHA52_001459"
     CDS             24327..25421
                     /locus_tag="ACHA52_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8940186.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8447 a         4424 c         5383 g         7242 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgtgcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tatagg
//
LOCUS       DAYYGY010000037        25494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000037 DAYYGY010000000
VERSION     DAYYGY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.038"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACHA52_001460"
     CDS             complement(159..1469)
                     /locus_tag="ACHA52_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8940187.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACHA52_001461"
     CDS             complement(1481..2899)
                     /locus_tag="ACHA52_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8940188.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="ACHA52_001462"
     CDS             complement(3139..4707)
                     /locus_tag="ACHA52_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8940189.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="ACHA52_001463"
     CDS             complement(4711..5121)
                     /locus_tag="ACHA52_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8940190.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="ACHA52_001464"
     CDS             complement(5118..6041)
                     /locus_tag="ACHA52_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8940191.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="ACHA52_001465"
     CDS             complement(6038..6916)
                     /locus_tag="ACHA52_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8940192.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="ACHA52_001466"
     CDS             complement(6903..8240)
                     /locus_tag="ACHA52_001466"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8940193.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="ACHA52_001467"
     CDS             complement(8243..9346)
                     /locus_tag="ACHA52_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8940194.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="ACHA52_001468"
     CDS             complement(9420..10928)
                     /locus_tag="ACHA52_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8940195.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="ACHA52_001469"
     CDS             complement(10894..11586)
                     /locus_tag="ACHA52_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8940196.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="ACHA52_001470"
     CDS             complement(11608..12582)
                     /locus_tag="ACHA52_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940197.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="ACHA52_001471"
     CDS             12882..13754
                     /locus_tag="ACHA52_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940198.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="ACHA52_001472"
     CDS             complement(14212..16410)
                     /locus_tag="ACHA52_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940199.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="ACHA52_001473"
     CDS             16642..17634
                     /locus_tag="ACHA52_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8940200.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="ACHA52_001474"
     CDS             17694..19115
                     /locus_tag="ACHA52_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8940201.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="ACHA52_001475"
     CDS             19108..20607
                     /locus_tag="ACHA52_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8940202.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="ACHA52_001476"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="ACHA52_001476"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8940203.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="ACHA52_001477"
     CDS             22199..22882
                     /locus_tag="ACHA52_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8940204.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="ACHA52_001478"
     CDS             22884..23720
                     /locus_tag="ACHA52_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8940205.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="ACHA52_001479"
     CDS             23731..25485
                     /locus_tag="ACHA52_001479"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940206.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKNQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7781 a         5187 c         4471 g         8055 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgattg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaacttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagata tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcttcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaaaccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aata
//
LOCUS       DAYYGY010000038        25245 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000038 DAYYGY010000000
VERSION     DAYYGY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.039"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHA52_001480"
     tRNA            12..84
                     /locus_tag="ACHA52_001480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHA52_001481"
     tRNA            88..160
                     /locus_tag="ACHA52_001481"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHA52_001482"
     tRNA            168..249
                     /locus_tag="ACHA52_001482"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHA52_001483"
     tRNA            267..339
                     /locus_tag="ACHA52_001483"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHA52_001484"
     tRNA            351..422
                     /locus_tag="ACHA52_001484"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHA52_001485"
     tRNA            439..524
                     /locus_tag="ACHA52_001485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHA52_001486"
     tRNA            535..608
                     /locus_tag="ACHA52_001486"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHA52_001487"
     tRNA            618..691
                     /locus_tag="ACHA52_001487"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHA52_001488"
     tRNA            711..783
                     /locus_tag="ACHA52_001488"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHA52_001489"
     tRNA            811..884
                     /locus_tag="ACHA52_001489"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHA52_001490"
     tRNA            898..971
                     /locus_tag="ACHA52_001490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHA52_001491"
     tRNA            995..1084
                     /locus_tag="ACHA52_001491"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHA52_001492"
     tRNA            1097..1170
                     /locus_tag="ACHA52_001492"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHA52_001493"
     tRNA            1189..1264
                     /locus_tag="ACHA52_001493"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHA52_001494"
     tRNA            1266..1336
                     /locus_tag="ACHA52_001494"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHA52_001495"
     tRNA            1350..1423
                     /locus_tag="ACHA52_001495"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHA52_001496"
     tRNA            1448..1535
                     /locus_tag="ACHA52_001496"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHA52_001497"
     tRNA            1557..1628
                     /locus_tag="ACHA52_001497"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHA52_001498"
     tRNA            1651..1740
                     /locus_tag="ACHA52_001498"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHA52_001499"
     tRNA            1753..1826
                     /locus_tag="ACHA52_001499"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHA52_001500"
     tRNA            1845..1917
                     /locus_tag="ACHA52_001500"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHA52_001501"
     tRNA            1921..1993
                     /locus_tag="ACHA52_001501"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHA52_001502"
     tRNA            1998..2068
                     /locus_tag="ACHA52_001502"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHA52_001503"
     tRNA            2081..2154
                     /locus_tag="ACHA52_001503"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHA52_001504"
     tRNA            2166..2237
                     /locus_tag="ACHA52_001504"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHA52_001505"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHA52_001505"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8940207.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHA52_001506"
     CDS             3826..5298
                     /locus_tag="ACHA52_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8940208.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHA52_001507"
     CDS             5381..6169
                     /locus_tag="ACHA52_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8940209.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHA52_001508"
     CDS             complement(6236..6904)
                     /locus_tag="ACHA52_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8940210.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYNLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHA52_001509"
     CDS             7019..8731
                     /locus_tag="ACHA52_001509"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940211.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACHA52_001510"
     CDS             8734..10503
                     /locus_tag="ACHA52_001510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940212.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ACHA52_001511"
     CDS             complement(10708..11274)
                     /locus_tag="ACHA52_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8940213.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ACHA52_001512"
     CDS             complement(11274..12230)
                     /locus_tag="ACHA52_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8940214.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ACHA52_001513"
     CDS             complement(12230..12886)
                     /locus_tag="ACHA52_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8940215.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ACHA52_001514"
     CDS             13256..15274
                     /locus_tag="ACHA52_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8940216.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /gene="leuS"
                     /locus_tag="ACHA52_001515"
     CDS             15701..18115
                     /gene="leuS"
                     /locus_tag="ACHA52_001515"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8940217.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="ACHA52_001516"
     CDS             18463..20016
                     /locus_tag="ACHA52_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8940218.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="ACHA52_001517"
     CDS             complement(20397..22181)
                     /locus_tag="ACHA52_001517"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940219.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="ACHA52_001518"
     CDS             complement(22196..23917)
                     /locus_tag="ACHA52_001518"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940220.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="ACHA52_001519"
     CDS             24017..24778
                     /locus_tag="ACHA52_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8940221.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7806 a         4638 c         5372 g         7429 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaattccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggttatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagtgaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcagcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatac
//
LOCUS       DAYYGY010000039        24827 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000039 DAYYGY010000000
VERSION     DAYYGY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.040"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1960
                     /locus_tag="ACHA52_001520"
     CDS             62..1960
                     /locus_tag="ACHA52_001520"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8940222.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2259..2978)
                     /locus_tag="ACHA52_001521"
     CDS             complement(2259..2978)
                     /locus_tag="ACHA52_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8940223.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    3168..3215
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3315..3647
                     /locus_tag="ACHA52_001522"
     CDS             3315..3647
                     /locus_tag="ACHA52_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940224.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3634..5637
                     /locus_tag="ACHA52_001523"
     CDS             3634..5637
                     /locus_tag="ACHA52_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8940225.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5656..6126
                     /locus_tag="ACHA52_001524"
     CDS             5656..6126
                     /locus_tag="ACHA52_001524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8940226.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6147..6731
                     /locus_tag="ACHA52_001525"
     CDS             6147..6731
                     /locus_tag="ACHA52_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFM8940227.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6746..7732
                     /locus_tag="ACHA52_001526"
     CDS             6746..7732
                     /locus_tag="ACHA52_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8940228.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7722..8033
                     /locus_tag="ACHA52_001527"
     CDS             7722..8033
                     /locus_tag="ACHA52_001527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8940229.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8055..9836
                     /locus_tag="ACHA52_001528"
     CDS             8055..9836
                     /locus_tag="ACHA52_001528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8940230.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9829..11205
                     /locus_tag="ACHA52_001529"
     CDS             9829..11205
                     /locus_tag="ACHA52_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8940231.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLLERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11212..11847
                     /locus_tag="ACHA52_001530"
     CDS             11212..11847
                     /locus_tag="ACHA52_001530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8940232.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11877..13232
                     /locus_tag="ACHA52_001531"
     CDS             11877..13232
                     /locus_tag="ACHA52_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8940233.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13424..14818)
                     /locus_tag="ACHA52_001532"
     CDS             complement(13424..14818)
                     /locus_tag="ACHA52_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8940234.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14910..15881
                     /locus_tag="ACHA52_001533"
     CDS             14910..15881
                     /locus_tag="ACHA52_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8940235.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15878..16660
                     /locus_tag="ACHA52_001534"
     CDS             15878..16660
                     /locus_tag="ACHA52_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8940236.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16829..17200)
                     /locus_tag="ACHA52_001535"
     CDS             complement(16829..17200)
                     /locus_tag="ACHA52_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8940237.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17334..17777
                     /locus_tag="ACHA52_001536"
     CDS             17334..17777
                     /locus_tag="ACHA52_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8940238.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17820..18629)
                     /gene="yidA"
                     /locus_tag="ACHA52_001537"
     CDS             complement(17820..18629)
                     /gene="yidA"
                     /locus_tag="ACHA52_001537"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8940239.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18648..20015)
                     /locus_tag="ACHA52_001538"
     CDS             complement(18648..20015)
                     /locus_tag="ACHA52_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8940240.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19999..20829)
                     /locus_tag="ACHA52_001539"
     CDS             complement(19999..20829)
                     /locus_tag="ACHA52_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8940241.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20992..21429
                     /locus_tag="ACHA52_001540"
     CDS             20992..21429
                     /locus_tag="ACHA52_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8940242.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21505..22125
                     /gene="rpoE"
                     /locus_tag="ACHA52_001541"
     CDS             21505..22125
                     /gene="rpoE"
                     /locus_tag="ACHA52_001541"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8940243.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22314..22820
                     /locus_tag="ACHA52_001542"
     CDS             22314..22820
                     /locus_tag="ACHA52_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8940244.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22837..23736
                     /locus_tag="ACHA52_001543"
     CDS             22837..23736
                     /locus_tag="ACHA52_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8940245.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23823..24707
                     /locus_tag="ACHA52_001544"
     CDS             23823..24707
                     /locus_tag="ACHA52_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8940246.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT         8081 a         4339 c         5297 g         7110 t
ORIGIN      
        1 attgaaaata gagcataaca actttataat gttgagtata ccaatttttg gaggcgtttt
       61 aatggaccgc tatttagatt tactttcaga gaagttccct acaacggaag atgtcatcac
      121 ggaaatcatc aatctcgaag caattttgca gcttccaaaa ggaacagagt tatttttgag
      181 cgatatacat ggcgaatttc ctgcttttga tcatattctg cgtatcggtt caggaaatct
      241 taaagaaaag atcaaagacc ttttctctga acaaatgacg gaaacggaga tcagccaatt
      301 cactattttt acggcttatc cagaatatgt gttgacgact gattggtaca aaaaacaaga
      361 taaatcccag ctgatccatc gtttgatcga tcttttacgg tttacgacag tcaaatacac
      421 tcgatcaaaa gttcgaaaag gattgccgaa agaatatagc tatatcattg aggaactctt
      481 gtatatcgat gatcgtatca acgggaaaaa agactatgta aaaaaaatca ttgaacaact
      541 tgtcttgatg aaggaagaag aaaaattttt aacaaaactt gcgcagacga tccaaaaatc
      601 tgtcatcgat catttgcata tcgtgggtga tatttttgat cgaggaactc aagcagccaa
      661 agtaatggat cgaatcatgg atttttcctc tgtagatatc cagtggggga atcatgatat
      721 tttgtggatt ggtgcgtatt gcggctctga ggcatgttta ttgacgcttt tacggattgc
      781 cgcacgctac aactatttat ttgaattaga gaaagaatat ggattaaatt tacgtccatt
      841 attttctttt gcccatcaaa cctatcagaa gaatccagta tttaccccta aaaacaggaa
      901 agatctatct gaaagagagc aagaagaatt agaaaagatc catcaagcac tttctatcct
      961 ccaattcaaa tcagaacatc agttgttgaa tcgacgacct gaatttaaaa tggacgatcg
     1021 taaattgttg gaaaaaatca actatgagga atcaacgatc gttttagaag gtcagctcta
     1081 tgagttgaaa gggacttgtt ttcaaacgat ccagccagat gatcctaaaa aattcttgga
     1141 agaggaacaa aaagtgatcg atagtttgat gtattcattc cagcactcgt taagaatgca
     1201 aaaacacatg acgtttttga tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt
     1261 actgttccat ggctgtatac ctgtagatga aaaggggcag ctcttagcat ttgaattaga
     1321 ccaatcttat cgcggaaaag aactgctggg attttttgaa aaacagataa ctgaaagcgc
     1381 aaaagatttg actgcaaatg aagatttggc gaccgatttg atttggtatg cgtggagcgg
     1441 gccattttcg cctttattcg gcaagagtaa aatggcaact tttgagcgct attttctcac
     1501 agagcctcat acgcacgtcg aaaaaagcaa tccctattat cacttgcgag atgaagagtg
     1561 gttcagtgaa aaaattttgg cagaattcga tgcaaaagaa gaaggttcag caatcatcaa
     1621 tggacacacg ccagtaaaag taaaaaaagg agagtcaccg atcaaagcaa acggaaaagc
     1681 atttgttatt gacggaggtc tatccaaagc ttatcaaaaa acaaccggga ttgctggcta
     1741 ctcattacta tgcaattctt acggtttcca aatcgtgaca cattatccgt tcttgcctat
     1801 tgaacaacaa tttgcaaaaa aatctgatca aacgaatgta aaaaaagtga tcgagcagca
     1861 gctcccacgc aaacttaatc ggaatacgac aaaaggaatg gaattacagc aacagattac
     1921 tcaactccaa aggctgttgg attatcgaaa ggacgattga gaaaaatgct agccaacatt
     1981 gctcaataga ctaaaaagcg actgcaacaa aaggcgtgtc gcagtctcct tcggaattag
     2041 ctcaaaaaac ggtaaaatat tagaagctat ttttctgttt atttctctaa tactcaggtc
     2101 agggcgctga tttcacgacc tcttccttaa acggtgttct cctgccaact cctcggtcac
     2161 aagtcacaat atccaacaaa catgagaagc tgttttttga aattgctcct tgtgactcag
     2221 agttgaacgg caggctcacg acctaatcag aaaaacgatt aaaattcatt cacaaagaaa
     2281 tcgaataaac gctgttcaga tgaatctaca cgatgagaaa gttccggatg ccattggaca
     2341 ccaaggattt tgtgcatatt atctttactt tggactgctt caatgagtcc atcgttggaa
     2401 aaggcgatgg cttttaacaa aggagaaaga tctttgactg cctgatgatg ataggaattc
     2461 acaaagtatg aatcagggag aaggtcactt aataaactgt ccgaaacgat tttgacttcg
     2521 tgagttgcaa actgcggggc agtgggctgt tgttcgtgtt ttacggacca ttttggatat
     2581 aatgaaagat cttgataaag tgttccctcc aacacgacat tcaatagttg aagtcctcgg
     2641 cagactgcaa aaatcggttt tctttgtttc aatgcttcca agatcaatgc ctgttcgaat
     2701 tggtctcgtt gaatatttgt ttccgtcagt tttggatgag ggtcttccat gtaaagttgc
     2761 ggactaacat cctgaccgcc tgcaagaatg agtttgtcga ttttctcaat tagttgaggc
     2821 gctgaatctg gagacatgac tggcagaata agaggtaatc cttgagcatc taaaactgca
     2881 tccacaaatc cctgcggtgt atagcttact tgatttcctt ggaaagtatc tgtcgctcga
     2941 atcagttgat tcccggcaat gccgatgatt ggtttcattt ttattccccc taaagtagac
     3001 taattgtaac aaggatttaa aaaaatttaa actatgtaag acttgcccct atacttatct
     3061 gttcttgctg tgttactatc tgtaaagaga attttcgtgt gaaattcatg acgatagttg
     3121 aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag
     3181 cttcaaggat ctagttcttc aaggggtgag aaggacattt tattgaaggg gcctgcctat
     3241 actaagttat ctttagaggt acatactttc aatgaaaaaa gaaagcatgt acctcttttt
     3301 taaatcgaaa ggaagtgaag caagtgttag aagctttgga acaaatccga aaagcagaag
     3361 aagaaaatga gaagcgtgct cgaaccctac ggcagcagtt gaagacctat gcagaagaaa
     3421 aagagcaaga attggagata atccaaacga acagtcggca acagctgaaa cagttggtag
     3481 aagaaaaaga acaattcgaa aagcaagaat tagaaaaaga aaaaatgaaa ttgaaagcag
     3541 aagcagacaa ggtgcaagca actttgaaag accaatatca aaagcatcag agtcaagcaa
     3601 tcggtgcaat cattgaaagg gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg
     3661 accctcattt cagataaaag aaatcaggag aggattttac aagcagtaca aggaatccaa
     3721 aatgttgaaa tccgggatct gtatcaaaat agaacaaaca accagtgggt agaaaaatat
     3781 tttccagata ccacgattat tgataaagaa gcaaaattaa gcgctttagg caatcggctg
     3841 aaagacatcc aagaagctat ccaattcata caacaccacg gcgatagccg tcaaaagaaa
     3901 ctccatttga aacgtcaaga actttcctta caagacttgg aagcaaatta ttccgaagaa
     3961 aatttcatga ataaattaga agaagtcctt gatttaaaag aaaagtggga acagctaact
     4021 gaaaaacgtg atcgtttaga agaagaggaa gaatggcttc ttcattggca acagctggat
     4081 attgctccaa gtatgtatgc aagtaagacg accatttttg agatggggac tgtgagtgca
     4141 gcaaattttg aaagtttcaa ggaagaacta agtaagcttg atgacgttta tatggaagaa
     4201 atagattata cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa atcagttgct
     4261 gctatagaag aaattgctgg gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa
     4321 aatcctaaag aaaaattgca agaaaccaaa cagcaattat ccattgttca cgagcagcaa
     4381 aaaaagataa cgacagcgtt aggtcagtgc agcggctata tccaagaatt tgaatgggta
     4441 gaagaagtca cacttgccgt tgcagaacgg gaaaaagtaa aagaacggtt tatccatgcc
     4501 acttatctga tcgttttgca aggttgggta gatgcagaag aaaaacagaa tttgatgcat
     4561 gtgttaaacg aaactgtatc agaagaagat atatacgtta gtttcgaaac accaaactca
     4621 gaagagattc agcaggaagt ccctactaaa ttgaaaaacc atccgcttat cgaacctttt
     4681 gaattgctga ctgaaatgta cagtctgcca aaatatgaag aagtagatcc aacgccttgg
     4741 atgacaccat tttatcttgt cttcttcggg atgatggtcg cagatgttgg ttacggattg
     4801 ctgatgctca ttgggacaat actagctcaa aaactatttg ttttaccccg cgggatgaaa
     4861 cgtttcgtaa aattctttga gattctctcg gtaccatcga tcatctgggg attgatctac
     4921 agttcatttt tcggacaggc attaccaaaa gaactatttg gtatccgctt gcctttccca
     4981 atcctatcta cgacggatga cgtcaatacg attttgattt tatccgtgat tttcggtctg
     5041 atacagatct tggttggatt attcgtggca gcaaaggaaa atatcaggag gaaagattac
     5101 ttgagtgctg tcagtgatgg ttttgcctgg caagggatcc tgattgggat cgttattgca
     5161 ttgattggtg tcatgttgat aaaaaataca gcatttgttt atcttggcgc ggggctatcg
     5221 atcattgctg ctttatgtat cttaatcgtg ccgattatcc aatcgccgtc taaagtcaag
     5281 ggtgctgcca aaggagcgta taatctttac ggattgactg gttatatcgg tgatttagtc
     5341 agttatacac gtttgatggc actaggtatt tcaggcggaa gtattggcgc ggcattcaat
     5401 atgctagttg ctttcatgcc gccagccgct cgattcagtg tagggatcct tttgatcatc
     5461 gctttacacg cactgaatat gtttttgaca ctgttaagtg cctatgtcca tggtgcacga
     5521 ttgcagtatg tcgaattttt tggaaaattt tatacaggag gcggacgtgc ctttgacccg
     5581 ctgaaaacgg cggaaaaata cgtcaacatc aatcataaga aaaaaaacaa taaataattg
     5641 gaggattttt atacaatgat ggattactta attagtcaaa atggtggaat ggtatttgca
     5701 gtattagcga tggcaacagc gacgattttt tcagggattg gttcagctaa aggtgttgga
     5761 atgacaggtg aggcagcagc agcgttaaca acaagtcaac ccgaaaaatt cggacaggct
     5821 ttgattctgc aattgcttcc aggtacgcaa gggttgtatg gattcgttat cgcctttttg
     5881 atttttatca acttaggaaa tgatatgtca gttgtacaag gattgaactt tctaggcgct
     5941 tctctgccga ttgcttttac aggtttgttt tcaggtatcg cccaagggaa agttgcagca
     6001 gcaggtatcc aaattttagc gaaaaaacct gaacatgcta caaaaggaat catttttgct
     6061 gcgatggttg aaacatatgc gattttaggt ttcgttattt ccttcctatt agtattgaat
     6121 gcctaagcat aaaggaggaa aagcagatga acgccattga aaaaatcatc tctcagatga
     6181 atgaagcagc agagcaagaa agagcggcgt tagagcaaga agagcgaatg aagatcgatc
     6241 aaaactttga acaaaaacgt acgcaaatcg aaacagaaca tcaaaaacaa aaagaaaaac
     6301 aaatcgaatt acttgaaaaa aaataccgtc agcttaggaa tcgccaacaa gtagaagtac
     6361 gacaagaaaa tttgaatgcg aagcaggaat ttttacgacg attgtttgca gatgcaatag
     6421 ctgaaatgga gaattgggat gaatctgaac aaatccagtt tatcaaaaat tcattgtatt
     6481 cattaccttt gacaggcaaa gtaacattca ttgctggaga aaaatcagca gcttatcttt
     6541 ctcagacatt gctggatgaa tggaacaatg aacttccctt tatgatggtt ttaagtgatg
     6601 aaactgttgc tgatcaagcg ggttttctga taaacgatca aggagttcag tataattttc
     6661 ttttcagcag ccttgtacaa gatatccaag gaacgatgag ttttgagatt gccaatcaat
     6721 tattcgaata aggagggatc agtgtatgga ataccacgaa ctaaatccct tgattcgcgg
     6781 aagagagctt gaacttttgt ccaatgagac atttgaacgg atgattcaaa caaattctgt
     6841 cgaagcactt ggtgaaatat tgaaatccac gatttacagt ccatatattt atgacggata
     6901 tgaatatgac ttcgaacaga atcttttaaa ggagcagagc cgtctatttc gctggttaaa
     6961 ggaatcagca ccggagcctg aaatcgtttg gatctatacg atgcgctata ctttccataa
     7021 tctgaaggtt ttgacaaaag ccgagatgac aaatcaaaat ctggaccatt tgtatatcga
     7081 cgatggattt tattcattag aaaccttgaa ggatgccatt catacgcaag tttcaaatga
     7141 actccctgaa agcattatgg acagtatacg agaagtacgt gattattttg aagagtctac
     7201 cattcttcag ggaattgacg tgatttatga tcgacagttt ttgacagagc agcgacggct
     7261 tgccgataag ctaggatatc aagaattgct gacagaagtc attgctttta ttgacttgac
     7321 caatatcaca acgatggctc gaggaatatt gcagaaaaga acatatgggt tcatgacgac
     7381 ggtactttcc agctcgggaa gtatcgaaaa agaagtattt ttgaactttg tagataaaga
     7441 tatggaagat tacacgaatt ttctattaga gacagattat gctgattttc tgcgccctgt
     7501 catccgtgag ggaaatcttg atctcgtaaa gcttgaacag ttgaaagatg attatctaag
     7561 ttcattgtat caagaagcac aaacccaggc tttcggacca ctgccactac tagccttttt
     7621 gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg gttatcggca aaagaagcca
     7681 tttcgaacca gaaacaatca gaaagcgggt gagacaagtc tatgacgcat aagatcggtg
     7741 tagttggcga caaagattct gttttgccat tcaaattgtt tggctttgat gttcgttatg
     7801 gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg
     7861 tgatttatgt cacagagcaa tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg
     7921 aacaaatgat acctgctatc gtattgattc ccaatcatca aggatcatta gggatagggc
     7981 tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca aaatatctta tagtatgaaa
     8041 aggagataga aattttgcaa attggaaaaa tcataaaagt ctccggacct cttgtaatgg
     8101 ctgagaacat gtctgatgcc agtatccaag acatgtgtct agtgggagat ctaggagtta
     8161 tcggagaaat tatcgagatg cgtggcgatg tggcgtctat tcaagtatat gaagagactt
     8221 ctggaattgg tccaggcgaa ccggttcgct ctactggtga agctttatct gttgaattag
     8281 gaccagggat catttcacaa atgtttgacg gaatccaacg gccattagat acctttatgg
     8341 aaatcacaca aagtaatttc ttagggcgcg gagttcaatt gcccgcattg gaccatgaga
     8401 aaaaatggtg gttcgaacca acagtagaag ccggagaaac ggtgagtgct ggagatgtta
     8461 tcgggatcgt agaagaaaca aaagttataa aacataaaat catggtgcca aatgggataa
     8521 aaggaacgat caaaacaatc gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca
     8581 ttgaaaccga aaatggtgac aaaaatttga cgatgcttca aaaatggcca gtaagacgta
     8641 gtcggccaat caaagaaaaa ttaaacccgg atgcacccat gatcactgga cagcgagtga
     8701 ttgatacatt cttcccagtg acaaaaggag gagcagcagc tgtacctggt ccttttggtg
     8761 cagggaaaac agtcgttcag catcaaattg caaaatgggc cgatgtggac atggttgtct
     8821 atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat
     8881 tgattgaccc taatacagga gaatcgttga tggaacgtac ggtattgatt gctaatacat
     8941 caaacatgcc agtagcagct cgagaagctt ctatctatac gggaatcaca attgcggaat
     9001 atttccgtga catgggttat gacgttgcta tcatggcaga ctccacttca cgatgggcag
     9061 aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag
     9121 cttatcttgg ttcacggctt gcggaatact atgaacgatc tggaagagtc attgcattag
     9181 gatcagaagg tcgcgaagga agtataaccg caatcagtgc tgtatccccg tctggagggg
     9241 atatttcgga accagtcaca caaaatacat tgcgggtcgt aaaagtattc tggggtcttg
     9301 attccagttt agcccaaaaa agacattttc cttcgatcga ttggatacaa agctattctc
     9361 tttattcaac agaagtaggg aagtatatgg atcatatcct tcaagctgac tgggcatcca
     9421 tggtcaccga gggcatgcgt attttgcagg aagaacagca gctgaatgaa atcgtgcgtt
     9481 tggtagggat tgattccctt tcagataatg accgtctgac attagaagtg gcaaaatcca
     9541 ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt cgatacattc acttcaagag
     9601 agaaacaatt caaaatgttg aagatgatct tgacctttgg tgaagaagga cgccgtgcgt
     9661 tgtcgttagg tgcttattta agtgagatca tggacggtac cgtatcgatt cgtgaaagga
     9721 tcggacgaag caaatatatc ccggaaacag aattagaaaa actagatcaa attattggtg
     9781 aaatcaagca aacgatgcaa acgatcgttt cagaaggagg catgagcaat gattaaagaa
     9841 tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc
     9901 aaatatgaag aattaatcga agtccgtatg caaaatggcg agattcgtcg tggacaagta
     9961 ttggaagtcc aagaagataa agcaatggtc caaatattcg aaggaacaag tggtatcaat
    10021 ttacgtgact cttctgtacg atttttaggc catcctttag aactaggtgt atcggaagac
    10081 atgatcggtc gagtattcga tggtttggga cgaccgaaag ataacggacc agagatcctg
    10141 cctgaaaagc atttggatat taacggagaa gtaatcaatc cagtagctcg ggattatcca
    10201 gatgagttta tccaaacagg tatctctgca atcgatcatt tgaatacatt agttcgtggg
    10261 caaaaattac cagtattttc tggctctggt cttcctcata aagaattagc tgctcagatc
    10321 gcgcgacaag caacggtact cgattcatca gatgatttcg ccgtagtctt tgctgctatt
    10381 ggaatcactt ttgaagaagc tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc
    10441 gaccgttctg tcatgtttat gaatttagca aacgaccctg caatcgaacg tatcgctact
    10501 cctcgtatgg ctttgactgc tgcagaatat cttgcttatg aaaagggaat gcatgttttg
    10561 gttatcatga cagacatgac caactatgca gaggcattgc gagagatttc tgcagcacgg
    10621 cgagaagttc caggaagaag aggatatcct gggtatcttt acacgaactt agctacttta
    10681 ttagaacgag caggaagaat ccgcggacta aaaggatcgg ttactcaaat tccgattcta
    10741 acaatgccag aagacgataa aactcatcca atccctgact tgactggtta tattacagaa
    10801 ggacagatca tcttgactag agagctttac aagagtggga ttcagccacc aattgatgta
    10861 ctcccgtctc tttcgcggtt aaaagataag ggaactggac ccggaaagac aagagaagac
    10921 catgcagcta cgatgaatca gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa
    10981 ttagccgtag ttttaggtga atctgcttta tctgatgtag acaagatcta tgcgaagttt
    11041 gctgagcggt ttgaaaaaga atatgtcaat caaggattct acacgaaccg ttcgattact
    11101 gaaacacttg atctagggtg ggaactgcta tcgatgttgc caagaaccga attaaaacga
    11161 atcaaagacg acatgctgga caaatatctg ccagaaggga agtgagttct gatggcacga
    11221 ttgaatgtga atccgacccg catggaattg actcgattga aaaaacagtt gacaactgcc
    11281 acaagagggc ataaattatt aaaagataag caagatgaac tgatgcgtca attcatcctg
    11341 ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag agtctatgac agaagcaatg
    11401 tctgattttg tattggcaaa tgcttcgatc acggaacctt tcatcgaaga attatttgtt
    11461 ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa atatcatgag tgtaaaagta
    11521 cctgtgatga attttgaata tgatgaacag ctgacagaca cacctttgga atacgggtat
    11581 ttgaattcca gtgccgaact ggatcggtca atcgatcggt ttactaccct gcttccaaaa
    11641 cttttggaac tgacagaaat cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag
    11701 acacgtcggc gagtcaatgc attagaatat atgacgatcc ctcaattaga agaaacgatc
    11761 tattatattc gtatgaagct ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa
    11821 gtaaaaaata tgggtaccac tgactaagtg gtatcttttt ttaaaagagg aaaacgatga
    11881 ttaaacaaag caaacaaata aagaaacgtt tgccggctgt tcagcttctc gcattaggat
    11941 tttttctcct gattttagtt ggcggcggcc tacttaccct accatttttt agtaatagtg
    12001 ggcatgggac aaattttatc gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg
    12061 gattgacaac attgaataca gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc
    12121 ttttgattga gatcggcggt cttggtttta tgatggttcc gattctatgc tttgcattga
    12181 taaagaagaa agtcagtctc agcacacgta ttatattaaa agaagcgctg aatctagaag
    12241 aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa attggccttt gttatccaat
    12301 tgattggtac ggttgcttta tcttttgtat ttgtgcctga atttggctgg ttgaaaggta
    12361 tctggtacag tttgttccat gcaatctcta gtttttgtaa tgcagggttt gatttgttag
    12421 gagacagttt agccaatcac cagcaaaatg tttatttgat catggtggta tctgctttga
    12481 tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt actggaatat catcagaaaa
    12541 agaagatgac gattcattca aaaatcgcat taaccgttac aggtaccctt ttattaggcg
    12601 gattcgttgt gttctttttc acagagcgca atggggcaac gctagtggaa gggaatatgt
    12661 ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc tagaacggct ggatattatt
    12721 cagttgatta cctccatatg agccatgcag gtctgatttt aacgatgttt ttgatgtaca
    12781 tcggcggaac atctgggtcg acggccggcg gattgaaaac aactacgtta ggtgtgttgc
    12841 tgatccagat gcgttcgatg ttgaaaggac gtacgagagc ggaagcattt ggccgtacca
    12901 tccgacaagg cgcagtgttg cgggcactta ctttattttt tgttacgttg acgttgtgtg
    12961 tagtcgccat catgatttta tcggtgactg aaacgatacc taaaacttct ggcatcgaat
    13021 atattgcttt tgaggtcttc tctgcgtttg gtacagtagg tcttacgatg ggattgacgc
    13081 cagacttgac tgcaataggg aaattgatca ttatagcgct tatgtacatt ggacgagttg
    13141 gtgtattgac tgtggtcttc tctctgatga caaaagcaca aagaacacaa tcaaaataca
    13201 aatatccaga agaaagtgtc atgattggat aatttcatct tctgacagaa acctagtgat
    13261 ttagtaaaat aagaaaataa aataattttt cttgcttctt ggatacttta agtgcaaaat
    13321 gttcgtttat tttttgaggc actacccgtt gttgaacctc gggtagtgcc tcttttttat
    13381 tgaaaggaat caataagatt tgattccttt atttatcaat aggttataat tcatattttt
    13441 ttattttttc ttttacgata cttagtccag aagctaaaat ttcaaggtta ttagaggata
    13501 gggaaattcg aacgaatgca tctggttgcg cacgtgtagt aaagcgtctg gaatgataaa
    13561 gtcggatacc gttagcaaga aagtcattct caattatctt agggtcaatc tcaggatcaa
    13621 ctggaagaat tcgatagtaa gggacacgat tcgttggttt ttctactttg aaaagagaat
    13681 caaaaagctg attggctctt tttgtaagac ttaatttagt ttctaagatc ctatttgctt
    13741 catttgaatg gaggatttga gtgatgactt ccacatctaa tccagaagtt ttcacattta
    13801 tattaaaaat tgcttgttct atcttgcttt ggacatgtac tggaactaca agataagcaa
    13861 ctcgcaatcc agaacaaata aattttgtca ttccagctaa gtagattgtt tggttaggta
    13921 ataaattttg gaaaggagga atgatcgtat gttgatagta agttgttaaa aatgaatgga
    13981 tatcatcttc aataacccat agaccttctt tttcaatcac ttcggctaat tgctctcgtc
    14041 ttttgtgaga gatttgaaac cctataggat tattacaaga aggcataaga aagacaccat
    14101 gaatcttctt ttttctacat tcctgaatca atatatcagg tcgcattcct tgctcgtcaa
    14161 aatcaattgg aacgatctct aaatgatata actgagccaa ttcaatgaaa ttagcgtatg
    14221 tatatcggtc tgttgctatt ctatcacctg gagagaaaag tgcagaaagt gtgatagcca
    14281 atccattttg aacaccggaa acaatagata gagattctaa ggacggaatg actccttgag
    14341 tttttaacca atttgctgca gtagtcaatt gaaaatttgt gcctttggga tcttggtaat
    14401 taagcaaacc aacaagattt gggttattac tagttgattg aataaacggg acaacgaatt
    14461 tattacattc ttcaaaagaa ctaaccagac caaaatcaat gatatgttcg gttacttcat
    14521 tggtagagat ggtgatggac tcaaagacat tgggagaaat aaatgtgcca cttccaatat
    14581 tcgtatacag caaacctttc tcaattccat atttataagc cttgccaacg gtagtaaaat
    14641 ttaaatctaa ataatcagct agctctcttt gggagggtaa ctgagtgttt ttctgtaatt
    14701 tattctttaa aatatcttgt tctaattgtt gaatcaacga tcgataataa ggacgcttca
    14761 atttttgttt gtctggtcgc caagttaacg ggtaattgtc ataagaatta ataggcattt
    14821 tctccactcc ttaaaattgt ataccataca attttaccat tgattgtatt gattttagaa
    14881 gttaatcttt tgtgaggagg gaattgtcta tgaaaattat taattttgag gaagtagtag
    14941 cttcttttag ttttgaagaa gcgattcaag taatgaaaaa atgttttttt gattatgaaa
    15001 gtggtaaaat cagccaaaat cctcgattgg tggaaatttt acctgacgga aaaggcaaaa
    15061 atatgtttgc gatgatgcct gcttatttgg gggagaacag atattttggc acaaaagtca
    15121 ttaccgcttt tccggataat catcagtatc atttaccttc tcatttagga gagattttct
    15181 tgtttgatgc tcaaaacgga ctacccaaag cattgataaa cgcaaatgca gtcacctgga
    15241 ttcgaacagc tgctgtatct gcgctggcaa cagattactt agcaaatccg catgcagaaa
    15301 cacttgcttt aattggagct ggtcaacaag cattgtcgca tttgactgcg atttctacca
    15361 ttcgtcccat cactacagtg tttgtttacg atattttaga agaaagacga aatactttta
    15421 tcgctaaagc taaaaaacag tatcctaaaa taacgtttat aaatgcaata tcagtagaag
    15481 atgcagtacg tccagcggaa atcatttgta cgttgacctc tagtaaagag gcatttcttc
    15541 aaaaagagtg ggtaagggaa gacgcacata tcaatgctgt gggcacattt actcctgata
    15601 caagagagat aagtacatcg ttgatggaaa cttctcaaat ctatgtagat gattactcag
    15661 ctgccttaac agaaagcggg gacctgctta tagccatggc agaaggagat ttagaggaat
    15721 cttgtattgt tggtacatta ggcgaattag tttctgagaa aaaaacattt tataaaggag
    15781 aatcgaggcc gacgattttc gacgcagttg gtctagctat tgaagatctt tgttgtgcag
    15841 aatatattta taacaaatta gagaaagggg ctttaaaatg aacttagatt attacgtagt
    15901 tgatgcattt gcagatgagg tgttcaaagg aaatccagca gcggtttatg tgctagaaga
    15961 atggctgcca gaaggtacga tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc
    16021 atttacagtg aagaaaaacc aagaatttga gcttagatgg tttacgccag atcgagaaat
    16081 cgatctttgt ggtcacgcga cattagcaac agcatttgtt ttatttaact attacaaaat
    16141 acctgatgaa acgattaaat tttctactca aagcggtaat ctatttgtga caagaattaa
    16201 agattattat tacatggatt ttcctagtat tatgccgaaa aaagtgccga tattgtctga
    16261 atatgaagaa gcgattgggg ctaagattaa agaagcctac ttagcacgtg atttattttt
    16321 cgtattagaa gatgaaaaaa cggtggctaa attgcagcca gattttacag ctatcaaaaa
    16381 ttttgaatta ggaataggag tcatcgtgac agcagaaagt gtgcaaaaag actttgtttc
    16441 tagaaccttc tttccaaaat taacaatcaa tgaagatcca gtatgtggat cagcacattc
    16501 taatcttatt ccttattggg cagaaagaat gaataaaaca aaattgtcag cttatcaaat
    16561 gtcatcaagg ggcggggatc tagaatgtga attaaaagaa gatcgtgtga taataggagg
    16621 aaaagcagtt ttgttttcta aaggtacctc gtttatttga tttctttcaa atgaattaaa
    16681 aataaattgg tggagagtgg caaccttata gctacaatgg ttgttaaaaa aagacgcaaa
    16741 ctttttcaat ttaatgaaaa gtttgcgtct cattttaatt attggtgttt gttttttatt
    16801 caatcaagag taaaatgccc catactaact atcctcggat gacttcgatt ttataaccat
    16861 cagggtcaat gacaaagtag taagaaggcg cagtgccagg taatcctttt aagtcagtaa
    16921 tctcaagacc ggcagcttta tgttgctcgt gtaatttttc tagatcatca gctgctatag
    16981 cgatatgacc atagccgtct cctagatcat acgcaccatg atcatagttg taagtcaact
    17041 ctaattcata gtcatcccca ggaagtgtca aataaacaag tgtaaatttg ttttctggaa
    17101 aatcacggcg gcggctttct tcaaaaccaa atgccttttg ataaaattcg attgatgctt
    17161 caagatcttt cactcgaaca catgtgtgag ccattttcat agattcatct ctccttttcc
    17221 ttttactcaa tcataacaaa aagaagtaag gaaatagttg atttgtgctt gttcatgaga
    17281 aatttctcat gtaaaatgga tagaaaataa aactgttaag gtggaaacta ttcatggata
    17341 aaccaatttt tggtgaaaaa aagcaatttg ctgattatca aacccgttat gcagcatata
    17401 ttgtcataga aagagatcag cagattgcag taatcgaggc tccaaatggt gctttttttc
    17461 ttcctggcgg ggaaattgaa ggagaagaga cgaaagaaga agcgattgtc cgtgagctga
    17521 tagaagaaat gggaattgtg gcagaaattt cttcttatct gggagaagct gatgagtatt
    17581 tttactcgaa ttatcgtgca acttattatt ataatccagg atatttcttt gttgctgatt
    17641 cttggaagcg tataggagaa ccaactgaaa agaccaataa aatttggtgg gtgagccctc
    17701 aagaagcgct tgtgaaattg aaacgtggta gccaccgatg ggcagtcgag agatggatcg
    17761 aaaaaaataa aaactgaaag aaagcgagta gatcagcttt ctctcagttt ttttatttat
    17821 tacagtacat attttttgat tgcttcagct acaccgtgct cgtcgtttga tgaagtgatg
    17881 acatttgctg cttctttgac aagagaaacg gcatttccca ttgctactcc tagaccagca
    17941 tattcaatca tggataagtc gttctcgtta tctccgatag ccatgatttc atcttgatca
    18001 ataccaaggt gctgtgcgag attcgcaact gccgcacctt tacttgcttc tttatttagt
    18061 acttcgaagt aatagggcgc acttttgacc gttgtgtatt tatcacggaa agtttgcggt
    18121 aaacgcgcaa ttgctgcatc taaaatttct ggttcgtcga tcatcatcat tttgacgatg
    18181 ttcatgtctg gtgtcatttc ttcaggtgtt cgatatttta gcggcatctt gactaaactt
    18241 gcttcgtgga tcgtataagc gctgatatct cgattaggcg tgtagatcgc tttgtcatcg
    18301 atcgaatgga gatgactgcc gacacggcgt gccattactt cgatctctag gaaatcttca
    18361 tgtgtcagtc cgtgacgggc gatgatttta cctgatttcg tttgttgtac gagtgccccg
    18421 ttataagtaa taacgtaatc attatcttga aaaaggttca actcgtttaa ctgttcctgg
    18481 acacctggta agggacggcc agtgcataaa acgatcgaaa caccttttgc aacagcagcg
    18541 gcaatcgctt cttttacttc ttttgttact tctcttttct catttaataa agtaccatcg
    18601 atgtcgatag cgactagttt gatagacata atttcctcca atatatttta atttatttca
    18661 atcaatgcgc cattacggat atggctggca aattcttgat aagtttcgtc aaataaatca
    18721 taatgatcac gcaaggacgg atcaaccatt tcttttggaa agaaaaatcg ctcatctcct
    18781 tgttcttgtc ccgctaatgc agcaacaagc tgactaacct gtgataattc tatcaaagtt
    18841 ccgtcatttc gcaaaatctc gatttgtgtg cgatgtctgc cttgttctgg gcgataaaaa
    18901 tcataaggta gatcgtagct agaattcaca gcagtgtaat aaacaggatt atatccgacc
    18961 ttttctacta acaaagtgag ttcttggact aattttgaat cccggttgcc agaaaaagta
    19021 gcagatttca gtggtttacg attcaaaaaa cgtttagcta aatcatttaa aataggatca
    19081 gcagattcag accattgagt aaagtaagtg cccaagaccc catcatcaag tttgaggtat
    19141 tcttctaaag taaagcttcc gtcaagaaat ggcagaagta attgagaatg gagttcaaaa
    19201 ctgctggcat cttcttgata caactcgtgt gcgcgatgaa gcagatgctc caaaattact
    19261 tccataccac gggaaacagg atggaagtac acttgcacat acatttgata tcggctaacg
    19321 atataatctt ccactgcatg catgccactc atggaaaaag cgatcccgcc ttcataagga
    19381 cgaatcacac gtaatattcg ggtaagatca aatgttccat attctgttcc agtgaaataa
    19441 gcatcccgaa gcaaatagtc cattcgatct gcatcgatct gactagaaat catttggaca
    19501 acttgtgggt tggggtaagt tttttggatc acactagcta ccttttcagg aaaaccttct
    19561 tctactcggt taagaatctg atacacttct gtttctggag aagtgatgat ctctacagtg
    19621 atggcttcat gattcgtatg gaagatatgt tcaaatgtat gagagtaagg accatgaccg
    19681 acatcatgca acagtgccgc acataaagtc accagccgtt cctgatcatc ccagcctccg
    19741 ttgcctattt tttcttttga aaaatttcga gaaaagatat cgcagattcg tcttgaaatc
    19801 tcataaacgc ctaatgaatg agtaaaacgg ctatgttctg ctccgtgaaa agtgaaagaa
    19861 gaagtgccta attgtttaat gcgtcgtaga cgttggactt ctttcgaatt gatcaaatct
    19921 aagattactt gatgctggac atggatatag ttatggactg gatcacgaaa aactttttcc
    19981 ataggcagca tttgatattt ataaggggtt gtcataatgt agctccttta tgttattacg
    20041 ttgttccatt ctctggattt ggttgttcca ttgctccagc tggatttctt tttttagcca
    20101 gtcggtagaa tcctgtaaat cacttgcaga atacagcttt tcaaaagctt ttgcaaatgc
    20161 ttctttgact tcttttacaa aaaggggttg gtcaagcagt tcttcaagtg ttgccattgt
    20221 agcaggatcc actgcaggat agccatttgt accaaagtct tcttttaaag cagattgata
    20281 aaatcgcttc atgacttgcc cacgtttttc ctgattaccg ttgacactta agtacatcat
    20341 aacagaaatc ccatttttga tccgacgctg agcgataccg gcaaattttt tgccatggat
    20401 gcttaaatca taagttccag gacaataaga atgggtgatt tcgaatgctt gtatcggtgc
    20461 ctttggaaaa gcgtatttag tcaataggag catttgctcg taagcgtcat ctatggttat
    20521 tttttttgtg tcactttggg ggaaaatcaa agaaatattc aaaattcctt catctgatac
    20581 gatccctaat ccacctgaat ttctgatcac tggagcgtaa cctgcatctt ccagtgtttg
    20641 gatcccgtca cttagaaaag gagttcgaac atccttcata cctaagatga ctgtgcagtc
    20701 atattgccag aaatgtaaaa agatttgctg gtgttcttta gaatattccg ttaatatatc
    20761 tgttaatgca aaaggggaaa agtcgttttt ttgataaatg tgctgatcta gtcgaatcgc
    20821 agggttcatt caaccggctc ctttgtataa attcatattt tctacaatat tatactctaa
    20881 aatcaaagag ttgttttgga aaaaaggttt tctgtaaaaa tggacaagtt ggttgacaaa
    20941 acaaaagaaa ctaggaacaa tgtagctatg agaagtaaag gagaatgaat aatggaatta
    21001 tcacaaggaa caccgatttc aatcaaattg cgtacaaaag taaaacagca aggggaagtg
    21061 caagattttt attttgattt gaaagggcaa atggtgacaa ttggcgatac gctgtacata
    21121 cgttataaag agattcagga ggagacgggc gatgagattc ctgtaacgat caaattagtt
    21181 ccagatggtc atgtacagct gatccgtgca ggagaaatgc ggatgcgttt gaaatttggc
    21241 tataaagaac ggttagaaac atcctatcga acaccatacg gtatgatcca gattgctact
    21301 tttacgaaag aactgcatgt tagtttgaaa gaccgtccca cagcagggaa ggttcgaatc
    21361 gattatgatc tgttcatggg accggaaaaa atcggcgaat attatttgac cttggatttt
    21421 acagcgtaaa tataaaagaa aagtttgatt ttttgtgtct gattttgtat gataaggagt
    21481 agactgtgaa aggacgtgtc ctgattggaa attagtgtat ttgaaggtgc aaacaaaagc
    21541 gaattatcaa tgatcgaagt agcacacgcg atcttagaac aacgtggcga tgtcatggat
    21601 ttttctgatt tagctaacca gatccaaaac tatcttggga aatcagacag tgaaattcgt
    21661 gattcactcg cacaatttta tactgacttg aatattgacg gcagctttat ctcattaggg
    21721 gataatcgtt ggggcttgcg ttcatggtat gctatcgatt cgatcgatga agaagtaaac
    21781 catggaatgg acgaagagga tgaagatact ccacgtcgcc gtaaacgcaa gaaagtcaac
    21841 gcctttatca atgatgatga agacgcgatc gattacaatg acgatgaccc agaagataca
    21901 gatctgacag aagaagatga cgatgatctg tttgatgacg atgatgatga agatgaagaa
    21961 atcgctgctt ataattctga cttgcaagaa atcggagcag atgacccgac agatgatgaa
    22021 gaagacttgc caggaaatat cgaagaagat ttaagtatca tagacgaaga cgatgatgat
    22081 gaagattatg aagaggaaga attttctgac tctgaaaaag aataaacatt attttcttac
    22141 gagaggttgt gacagagacg atcagcctat cagaataagg aggtgtgaca cgatttttgt
    22201 cacaccttct tttttgtata taaaaatccc aagtaaatac ataaaaaata aaaaaattga
    22261 aacttttagc gcaccttgta cgtcatatta agtgaggcag aaaggagtgg agaatgattt
    22321 ttactggata taagaaaaaa cggcagatcg aaaaataccg tcacctgctg aatggactca
    22381 tcgaaactga ttatcaaaaa atgttccgta tcacgatgaa ttacgtacat gataaagaag
    22441 aggccttaga tgtgatgcaa gatagttttc ataaagccct gaactatttt gaaaaagaaa
    22501 aagagatcga gcattttagt gcatggtttt atcggatatt gatccgcacg gcattagata
    22561 gttggcgcag acaaaaaagg acgcctgtgg acttatttga aattcaagat atcccccaac
    22621 agttaccttt agaaacttcc gttgcagagc tccatatgat tttagataaa atagacagtc
    22681 cggaaaaaga gattctgatc ctctactttt ttgaaggatt tcgtttgaaa gaaattgcga
    22741 ctatcttgga tatgaatgaa aacacagtta aaacgaaaat gtaccgctca ttgaatgctt
    22801 tacgtcaaat cctagaatga aggagagaag agtgccatgt attcccaaaa agaaaattta
    22861 aaaaaactaa agaaaactta taaagaacaa gcaattccca aagggcttaa agaggagatc
    22921 cgccagcgtt ttattatcga agagcaatca tttataaaag caaagcggag aaagagacgt
    22981 ctccaagggt tcggagtatc tttggctatc gtaatgatta caacaagtag ccttcttttt
    23041 aatagtcaag ttcgaagctt tgcagaagat ttacccatct taggatcagt cattgaattg
    23101 atcttaggcg aacgctttac tgatcgatct gaaaaaatcg atattcaggt accgaagatc
    23161 aatacacaaa atgagaaaga aaataaaaca attcatggat taaatcaaaa atactttcgc
    23221 gaaggacaag cagattttga aaaagctgaa aaagaatatg gaaattttga aacggatcat
    23281 taccaagttt tgggagatta tcagaaaatc gtagacgata accggtttct cgtgattgaa
    23341 aggcagatca cccaaacagc agcagatagc catgttgaaa aaagatacga cacaatcgac
    23401 aagaaaaaca gtgtccagct ctcacttcca cttttattca aagatgacac ctatctatct
    23461 gttttgacaa aagaagtcaa acggcagatg gccgaacaag tcaaggaaga tccatctaag
    23521 tactattgga ctgagcaaga tattcaagaa gggacgatag aaaaaccaac acttgttacg
    23581 cctacacgaa gtttttattt gaacaaagag catcaattag ttttgacttt ttctcaatac
    23641 gaaattgctc ctggttatat gggtacacca gaatttgtca ttcctaagtc cgtgacaaaa
    23701 acgattttgg catcggaaga ttatttggat cattagacga gaagtagttg cgactagcag
    23761 tttcaatttt caagtaagta caagtaaata ataagaagag aaaaagagaa agaagagata
    23821 tagtgaaaag aaattattta gttatcagta ttattgcttt atccgtgctt ttgtttttcg
    23881 aaattacttc ggtggcctcc ttgatcaccg aagtaaaagg acaaacacac tcttcggagc
    23941 attcgtcaca atctttcaaa caatcagaac aatcaaccat taaaagccaa tcaactgaaa
    24001 cagctaacgt caaagaagcc attcaagaat cagcggaaaa ggtacaaagt cagtcatcac
    24061 aatcaacaca agacagtaca aaaacgagcc gagatccggc tgtcaaaagc gtcgcaatca
    24121 gctttgatga tggaccagga gcgaccacaa caccgcagct tctgcgaatc ttaaaagaaa
    24181 aaaacgtgca tgccactttt ttcgtcttag gagaaaatac ggcgcagcat cctgaaatcg
    24241 tgaagcagac agctgaagca gggcacgaga ttggcaatca cacatacgat catcagaatc
    24301 tggccactct ttctgcccag tcgatgacgg aggaagtgac gaaagcagac actgagatca
    24361 aaaaagtcac tggcaaaacc cccacttttg ttcgtccgcc ctatggttct gtcacgaatg
    24421 taggagcaac gatcatccag cgaccgatca tcgaatggtc tgttgattca gaagattgga
    24481 agacaagaaa tcctgatttg attttgcaga agatccaagc aacagtatac gatggggcaa
    24541 ttattttatt ccatgatatc tatcctgaaa cgattcgtgc cgttcctcaa gtgatcgatt
    24601 atctgaaaga gcaagggtat cgcatcacga cagttgggga tttattaggg catccaacgg
    24661 ctgtcgagaa ctattatggc agaaacgatc atcggccagt tcagtaagtg agacaagtat
    24721 ctttaataaa taacacattt taataagtag ttgaacacat aactacctta cttgcaaagg
    24781 cttaacaata tctatacagg tacaacaaaa aactgtccac caatttt
//
LOCUS       DAYYGY010000040        24819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000040 DAYYGY010000000
VERSION     DAYYGY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.041"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..411
                     /locus_tag="ACHA52_001545"
     CDS             34..411
                     /locus_tag="ACHA52_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940247.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(591..1886)
                     /gene="purB"
                     /locus_tag="ACHA52_001546"
     CDS             complement(591..1886)
                     /gene="purB"
                     /locus_tag="ACHA52_001546"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8940248.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1899..3023)
                     /gene="purK"
                     /locus_tag="ACHA52_001547"
     CDS             complement(1899..3023)
                     /gene="purK"
                     /locus_tag="ACHA52_001547"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8940249.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3016..3504)
                     /gene="purE"
                     /locus_tag="ACHA52_001548"
     CDS             complement(3016..3504)
                     /gene="purE"
                     /locus_tag="ACHA52_001548"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8940250.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3920..5227)
                     /locus_tag="ACHA52_001549"
     CDS             complement(3920..5227)
                     /locus_tag="ACHA52_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8940251.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5230..5811)
                     /locus_tag="ACHA52_001550"
     CDS             complement(5230..5811)
                     /locus_tag="ACHA52_001550"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8940252.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5927..6024)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6383..7675)
                     /locus_tag="ACHA52_001551"
     CDS             complement(6383..7675)
                     /locus_tag="ACHA52_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8940253.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7672..8295)
                     /locus_tag="ACHA52_001552"
     CDS             complement(7672..8295)
                     /locus_tag="ACHA52_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940254.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8298..9134)
                     /locus_tag="ACHA52_001553"
     CDS             complement(8298..9134)
                     /locus_tag="ACHA52_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940255.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9151..10038)
                     /locus_tag="ACHA52_001554"
     CDS             complement(9151..10038)
                     /locus_tag="ACHA52_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940256.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(10054..11337)
                     /locus_tag="ACHA52_001555"
     CDS             complement(10054..11337)
                     /locus_tag="ACHA52_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8940257.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(12020..12703)
                     /locus_tag="ACHA52_001556"
     CDS             complement(12020..12703)
                     /locus_tag="ACHA52_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8940258.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12721..13338)
                     /locus_tag="ACHA52_001557"
     CDS             complement(12721..13338)
                     /locus_tag="ACHA52_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8940259.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13348..14172)
                     /locus_tag="ACHA52_001558"
     CDS             complement(13348..14172)
                     /locus_tag="ACHA52_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8940260.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14165..14725)
                     /locus_tag="ACHA52_001559"
     CDS             complement(14165..14725)
                     /locus_tag="ACHA52_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940261.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14899..15855
                     /locus_tag="ACHA52_001560"
     CDS             14899..15855
                     /locus_tag="ACHA52_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8940262.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16160..17116)
                     /locus_tag="ACHA52_001561"
     CDS             complement(16160..17116)
                     /locus_tag="ACHA52_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8940263.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17220..17822
                     /locus_tag="ACHA52_001562"
     CDS             17220..17822
                     /locus_tag="ACHA52_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8940264.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17910..19409)
                     /locus_tag="ACHA52_001563"
     CDS             complement(17910..19409)
                     /locus_tag="ACHA52_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8940265.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19425..19949)
                     /locus_tag="ACHA52_001564"
     CDS             complement(19425..19949)
                     /locus_tag="ACHA52_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8940266.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20247..20996
                     /locus_tag="ACHA52_001565"
     CDS             20247..20996
                     /locus_tag="ACHA52_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8940267.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20987..21772
                     /locus_tag="ACHA52_001566"
     CDS             20987..21772
                     /locus_tag="ACHA52_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8940268.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21741..22457
                     /locus_tag="ACHA52_001567"
     CDS             21741..22457
                     /locus_tag="ACHA52_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8940269.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22559..23578)
                     /locus_tag="ACHA52_001568"
     CDS             complement(22559..23578)
                     /locus_tag="ACHA52_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8940270.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23597..24598)
                     /locus_tag="ACHA52_001569"
     CDS             complement(23597..24598)
                     /locus_tag="ACHA52_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8940271.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7379 a         5324 c         4324 g         7792 t
ORIGIN      
        1 atttggttaa tgggttcgag gaggcacttg ccgatggaag atgcaataaa acaaggtcag
       61 ctttttttat cagaattaac agctgaagaa cgttcctgtc tatataatga tttgctgtcg
      121 atcaaagacg tttatttatg caataaagat actagttttt atacattccg aaaaatccgc
      181 cgagcaacca agcgctgtgc tgaatgccgt aaaaggactt ttctttatca gacagaacac
      241 aaaggcgaac tttcaatcag tcagattcat gaacagaagc gtttcaaaaa tcattatcgt
      301 atccagatct tattcaatgg acaagagatg gaatacgctt ttctcgaaaa cttgctgcaa
      361 catctccata accagcattt tagttataat agccaagcca tttacgctta attcctctat
      421 tttttgtatg taaagaggca cggtaaagag gtagccgctt ttaaaatcag gttcctatta
      481 ttgataagaa aatataataa actaaaagaa gcagaaccgc taacggatct gcttcttttt
      541 tggttatttt tgctattcta ttttaattga attttgcaac tcactcttct ttattccaaa
      601 cctactcgtt tgaatatcac gtcaacgttt ttcaaatgat gatgataatc aaaggcatcg
      661 tctatttctt ctttagataa taccgatgtg atggttggat cttcttctaa cagcgggcgg
      721 aactgcactt ggttgtccca agcgtacgca gtttttggct gaacaagatc ataagcttct
      781 tctcttgtca ttcccttatc gatcaatttc agcataacgc gctggctata aatcaaacca
      841 aatgttgcag acatattccg cttcatgttt tcaggaaaga ccgttagatt tttaacgata
      901 ttgccaaaac gatttaacat gtaattcaat aaaatcgtgg tatcaggcaa gatgattcgt
      961 tctgctgaag aatgagaaat atctctttca tgccataatg tgacattttc ataggcagtg
     1021 accatgtgtc cgcgcaccac tctggctaga ccagccatat tttctgaacc gatcggattc
     1081 cttttgtgcg gcatagcaga agaacctttt tgacctttcg caaaaaattc ttctacttca
     1141 cgtgtttcgg atttttgcaa tccgcgtatc tctgttgcaa aacgttcaat acttgtagca
     1201 ataagcgcca tcgctgagaa atattcagca tgtagatccc gagggagaac ttgagtagaa
     1261 atttcttgtg gacggatccc caattgttta catacatact cttcaataaa tggcgggata
     1321 tttgcgaacg ttcccactgc tcctgagatt tttcctgctt ctactccttt tgccgcatgt
     1381 tcaaatcgtt cgatgttccg tttcatttct gaataccatg tggcaagttt caatccaaaa
     1441 gtagttggtt cagcatggac accgtgtgtc cgacccatca tcactgtata tttgtgttct
     1501 tttgcttttt cgccaatgat ttccgtaaaa cgtttcaagt cttcccgtaa aatatcattt
     1561 gcttgcttga tgagatagcc ataagcagta tctacgacat ccgtacttgt tagaccataa
     1621 tggacccatt ttctttcttc tcccaatgtt tcagaaaccg ctcgtgtaaa cgcaacgaca
     1681 tcatggcgtg ttaatttttc gatttccaag atccgatcga tatcaaatga tgcatgctca
     1741 cgaatttttt tcacatcttc ttgcgggatt tcaccaagtt ctgcccaagc ttcatctgct
     1801 aaaatctcga cttcgagcca cgcacggtat ttgttctctt ccgtccagat attccccatt
     1861 tcagggcgtg tataacgttc taacatgttt tttctcctct aatcccaaat attcgtttgt
     1921 ttgatttctt ccagcgtttg ctggatgtct tctgtcaaga tagtgatatg tcccatcttg
     1981 cgaccttttt ttgcttgttt ttttccatag aaatggaatt gccagtctgg cttattctca
     2041 atcaagcgat agctttccag cagttgttct cccaaaacat tgaccattac tgcattgctt
     2101 atcaaacgta ctttttctcc taatggccaa ccacaaatac ctcgaatatg ggcatcgaac
     2161 tgactcatag aacaagcttc aatagaataa tgtccagaat tgtgaggacg aggagctaat
     2221 tcattcacat atagcccgcc tgtttttgtt aaaaacattt ctacccctaa aacacctgaa
     2281 aggctcactg cttctgcaat cacacgggca atacgctcag cctcctcgat cacatcctga
     2341 tcaatcgcgg ctggagcaat cgtttcatga agaatattgt tgtggtggat gttttccacg
     2401 accggaaaag tcgtgaaatc tccttgacca tttcctgcca ccatgatcga aatctctttt
     2461 tcaaacggga tccatgcttc taaaacacag gttccttctc ttaaaaggtc catggcagga
     2521 gctagatccg cccgactttt caaaacatac tgccccttgc catcatagcc gcctcgagtg
     2581 gttttcaaaa cacaaggata gccgatcccg tcaatcgcat cttggatatc cgtcggactg
     2641 acgatcgttg cataaggagc aatcacgatg ttattcgttt ccaaaaaaga tttttctaac
     2701 aggcggtctt gcgtaatcgc aagcaagtct gtcccttgag gaataaatga catgggtaaa
     2761 atcgcattta acgcctctac actgacgttt tcgaattcat aggtaatgac atctgttctt
     2821 ctggcaagct ctttcagcgc aaaggtgtca tcatagacac tttcaatatg ccaatctgca
     2881 acttgtgcgg ctggacaatc gctcacagga tccagcacac ctactttaaa gcccatttct
     2941 tttgcactaa tcgccatcat acgacctaat tgaccgccac cgacgatccc gatcgttgaa
     3001 ccaggcaaca aaggtttaac caagttgatc actactttct ataacagttt tagccaacat
     3061 tgaacgtctt tcagccaatt tagcctccaa ttctaaatca tacatggaaa gcatctgtat
     3121 cgcaagtagt ccagcattaa ttgctcccgc ttttccgatt gctgtagtag ctacaggtac
     3181 tcctccaggc atttggacaa tcgaaagaag cgaatccaaa ccatttaatg ttcgtgattg
     3241 gaccggtaca ccgataaccg gcaaagttgt ttttgatgcg accatacccg gcagatgcgc
     3301 ggctccccct gctcctgcaa taatgatttt gattcctttt tttcgagcgt tttgtgcata
     3361 ttcaaacatc aaatctgggg ttcgatgagc agaaacgacc tttttttcat aagacacacc
     3421 gaattcatcc aatatttgac atgcttcttt cattgtttcc caatctgaag tactccccat
     3481 aatcacagag atttttgctg tcattgtttt tcctcccagt ttttctacat tcatatttta
     3541 acaatcactt tttttgatgt caaagaaatt tcgaatgtta acaaagaaaa acattttatt
     3601 gttcgtgttt attaaagata aaaaaaagga atgtgacaaa agttaggtca cattcctttt
     3661 tatggataaa cggtgttctc ctgccaactc ctcggtcaca aggaacaata ttcaacaaac
     3721 atgagaagct tttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga
     3781 cctcttcctt caacggtgtt caatcagctg accctcggta ttagctcaaa caaacggcaa
     3841 aatacgagga actatttttc cgtttgtttc tctaacaatc aggtcaaaac gctgatttca
     3901 caacctttct tttgctctat tacttcttga aggcttcttc caatatttct tcatcttttt
     3961 tcaatccatt aaagatcaaa ttcaatatga tagccgtaat gctactcatt acgatgccat
     4021 ttccagtgaa catttgcaat gtttgcggca tttgactgaa taatgtcggc gtaacattga
     4081 aacccagccc aaatccaaca gaaacagcaa caatcaatag atttttatca ttgctgaaat
     4141 caacttccaa cagcatacgg attccttgga ctgccaccat cccgaacatg actaacattc
     4201 ctccaccaag taccggctca gggatgattt gtgctaacgc accgatttta gggaataatc
     4261 ctaaaacaat caagaagaat gcagagaaat agatgggttt tcttgttttg attcccgaaa
     4321 gttgaaccaa tccgacattt tgagaaaagc cagtataagg gaatgtattg aaaataccgc
     4381 ctaagataac ggcaagtcct tctgcacgat atcccttttt caattcttct tctccaacat
     4441 ttttcccagt aatgtcacca agtgcaaaat atacacctgt tgattctacc atgctgacca
     4501 tggaaatgat gatcattagt aaaatcgacc atacgtcgaa agttggttta ccaaaataaa
     4561 agggttgtgg aacatggaac aacggcgcat ctccaatcgc gctcagatca atcattccca
     4621 tgaaagcagc taagatgctt cctccgacta aaccaatcaa taccgcgatt gaacgaatga
     4681 acccttttgc cagcatttgc acgcctacaa ttaggagaat cgtcacaaat gcaagtagca
     4741 gatttgtttt gtcaccaaaa tcagctgctg ttgcattacc tccacccatt ttctcgattg
     4801 ctacaggaat caaagtcaaa ccaataacgg tgataaccgt tcctgttact ataggaggga
     4861 atagtttttt aattttagaa aagacaccag aaactaacac aacaaagatc ccagaagcga
     4921 taatagatcc gtagatagct cctactcctt cattggttcc gatcaagatc agtggagcta
     4981 ctgcttggat cgcacatcct aaaacgacag gcaagccaat tccgaaaaat cggttaactg
     5041 tcaattgcaa taatgtcgct aatccacaca taaagatatc aattgaaatc aaataagtca
     5101 tctgttcttg attaaagcct agtcctgtac cgatcaaaag aggcaccgct actgctcctg
     5161 catacatcgc tagcagatgc tgcagcccta aaaccgcagc ttttccattt tgtgtttctt
     5221 tttccacgat tatgcgtcct cctcaagaaa ctcaacttgt ccattcgtca aggaagcaat
     5281 acgcgcaaga gaaacgactc tcaatcccat ttcttcaagt aactgacgtc catcttggaa
     5341 agacttttcg atgacgatcc cgattccttc tacttgcgct ccagcttgct ggcataattc
     5401 gatcaatcct tttgccgctt gtccgtttgc caagaaatcg tcgataatca atactttgtc
     5461 ttctgaagat aaaaatttac gagaaataga aatcgtactt gtcacttgtt tcgtaaaaga
     5521 ataaacagaa gctgtcaata attcttcatc catcgtcaaa ctttttgctt ttctagcgaa
     5581 gatcattgga acatctaatt ctcttgcagc aaataaagct ggtgcgatcc cagaagcttc
     5641 aatggtcaca actttcgtga ttccttgttc tttaaatact tctgcaaacc gttttcccat
     5701 ttgttccatc aatacagggt ctacttggtg agtgacgaag ctgtcgactt ttaatacgcc
     5761 ctcgcctaat atacgtccgt ctttttgaat acgttcaact aattctttca ctttatttgc
     5821 tcctttatca tttattaata ccgaaataac cgcttttgtg caaaaaccta ctatgtttct
     5881 gttaaagaaa aacaccctcc actttatcat aagaaaagaa gggtgaaata gccaatcttt
     5941 tacttatagt ccagcattta cggtactggc tagaaactgt catccatatt atgacgatat
     6001 ataagtaggt atgtaaaact aataatagtg taaattcatt ttcttgcaac tgttttgcac
     6061 gtgaaggcag tttgccatat ttgaataaaa aatgttgttt caaatattga aaacagtcta
     6121 acactacttt ctgaaactga caacgtggtt tacttatttt tttgtaaaat ctaatgattt
     6181 ttaatgcatt attccttttt gttcgggttt aaatagttat ttttattttc tcctatctaa
     6241 aaaagcagcc cacataaaaa gaaggcatga caaaaagcat gtcatagccc ctttttccga
     6301 ataaatagtg ttcaaaagac agcctatcgg caataaggca agattttata aactacggga
     6361 agtaatttcc gtaaatctat tcttattctt ttaagctgac aagctcgtca atcactactg
     6421 cttttcctga agcaatagaa tgatttgctg cgattcctgt caaaatcgac aaaataccat
     6481 ctacgtgagt tgctgcccga ttccacggat cttcttcttt ttcttcacca aatagatcat
     6541 ctaataaacg cggatctcct ccgccatgtc cgcccgcttc ttgttcgacg attacctcat
     6601 atggctcacc gaacatcgga tagacatgga tggactgaag aacggctgct ccttcatcct
     6661 ttttatctcc acctgcattg atgtaagact tttcgatcac tttcatttcg atccgtcctt
     6721 tgctgccatt gaaagaaaca ttgaatcctt cccacggcat atacgcgttt agcgaatacg
     6781 tcaaaatcgc accagtttca tatttgaccg ttacgcctag tgtatcttca atagagatat
     6841 catcggaaaa tacactgcgg tcacgaatat atccactgtc tttttctgct tctaaataca
     6901 tttctcgcaa ttcctcattg gctgacaggt ctatagcaaa tggatcagct ttagctagtt
     6961 cgcttccata gcatcgactg taaaatattt ctactccgcg tttctccgca ttatgcatcc
     7021 cataaaagtt taatccaccg aatgcaaata ccttttgggg acggctgttc aaccaaaaat
     7081 tcactagatc aaaatgatgg gtagatttat gaacaagaag cccgccgcta ttgtgcttat
     7141 ttcgatgcca tcttctgaaa taatctgccc cgtgttgcgt atttaacagc cattcgaagt
     7201 ggatagaaaa cacatcacca atcacatcac tttctatcag ttcctttatc ttcgtgtgat
     7261 acggtgcata acgataatta aaagtgactc gcaggcttct tcccgtctgt ttttggaccg
     7321 ttattatttc ttgagctttt tcctcatcga tagtcagagg tttttcgcaa ataacatcat
     7381 atcctttttt cattgcacga atgatataat catgatgcgt tcgatctact gacgtaataa
     7441 tgacttcagc cggccgtacg tcatccagta gttgctcaaa ttgtgatggc atacatgtct
     7501 ttactttttt ctgtccatac ttgttcacaa gtatccgatt tgcataatcc atccttgttt
     7561 gagatacatc acaaaaaccg actagttcac aagtttgatg atacgtagag gcaattgcct
     7621 cataaaaaaa acgcgctctc ccaccaagtc cgacttgtac atattttttc ttcatttaaa
     7681 gtcattctcc ttttctttta agcaaaatac tgtgacaaat ggcagccctg gatacagtaa
     7741 tgcagtgagt aaaaaatcat gctgtataca ccagaacagt ccatacaaaa ggcataaata
     7801 ccacagaaac cccttgacgt tttttgacaa cagccagata ttccttttga tcaaataaac
     7861 aagactatct gctttatctt ctttgcagat atcaattagc gtactaaaaa aactatggga
     7921 tacaaaaagc aacagacaca atagaaaagg aatcagatac caagctccaa tctttcccac
     7981 taataaatac caatcaactg caaaaataat gacaaacagc tgaaacaacg taatgatgaa
     8041 acacatccgt ttgatttttc ctttcctcca acataccttt ttttctgcca agtaatggac
     8101 agatagataa taagaaggat acatcatcaa aaaataaatc agatagacaa cacgagcaga
     8161 cagcactaat cctaaagtga acaaacatcc tagaactaaa gcgaaaagag cattactgat
     8221 aagactggtt aaaaaaatcc gacagacata gtagttgagc ttgtaaagaa acatctcttc
     8281 tattggcttt ttcatattca gcccttcaga ccgcttgtac tgatcccctg gacaagatat
     8341 ttattgaaga ataagaaaat cagaaaaacc ggtagcagcg acaacgtaga catcgcaaac
     8401 atactcgcgt aagtcgtact agatccagcg tctgcaaata atttgatggc aatcgatacc
     8461 gtgtaagatc tcggcttgtt taaataaatc aatggtccca tgaagtcgtc ccatttccaa
     8521 taaaactgga tgatgatcgt cgtgatgatc gaaggtttca gcaaaggaaa aataattttg
     8581 gtaaagacac tatacttgct gcatccgtca ataatcgctg attcatctaa ctctttgggg
     8641 atgctgacca tgaattgcat gatctggtaa atgaaaaatg cttgtccaaa aaaatgtggt
     8701 aaaatcagcg gcacatacgt cccaaccaaa ttcagccgat tgtaaataat aaactgcgga
     8761 atcaaaatca cttgtgcagg aatcatcatc gttactaaca tgacgacgaa ccagaatctt
     8821 tttcctttga aatcgattct cgataatgcg taagcaatac aagtcgagct cagaactgtc
     8881 cctatcgtcg aaactgcaga gatgacaaac gaattcctaa agaaagtaaa aaatgaaatt
     8941 ccagcaaacc ccgcccatcc ttttgcgtaa ttttcccatt gaaaagtctc tggaatcaaa
     9001 ttcagcgaac cactcataat ctcttgatcc gttttaaaag aacctgcgat catccataag
     9061 accggataaa tcatgactag acctaatagc aaaataaaac cgtattgaaa ccatttcgtt
     9121 actgtattat tcatttcttc ctcctctcat tcacacaacg acccattttt ttgatgattt
     9181 gaaaatcaga aaggtgacag ctcccataat caacagcatg atccacgaaa tagcactagc
     9241 ataccccata gaattaaaag aaaaagcttg gttatacaca taaagagcat aaaagttcgt
     9301 ggcatcattc ggaccacctt gggtaatgat aaatgcctgc gtaaaagtca taaacgccgc
     9361 gattgtctgc ataaccaagt tatacaaaat gatcggcgaa agaagaggta acgtgatctt
     9421 gtaaaacgtc tgccatttgt tcgctccatc gatctctgcc gcttcataat acgagcctgg
     9481 gatctctttg atacctgcag caaagatgat cattgccgaa ccgaattgcc aagcagacat
     9541 caaaacaaga gggatcatga ccagtttttg atcgctaaac caggaaagcg caggtgcgcc
     9601 taatcgttcc aggaaaatat tcactaaccc gtttttagaa aacaactccc gccaaactag
     9661 agcgatagaa atactgccac cgatcaaact aggcacataa tacaaagaac gaaagaaagt
     9721 cgttcctttg atattcctcg ttaaaagata agcaatcaat agaccaaaga tcagtttcac
     9781 aggtacagat gcaaatacat agagaaaagt gactcggatg gagttcaaaa aacgggcatc
     9841 actcagcatc tttatataat tatccaagcc gatccatgag actggatttc ccaaacgata
     9901 atcggtgaac gaataataaa gtgacaagat catcggtatc actgttatca atagaaatcc
     9961 taggatgaat ggagaagcaa atacgtaacc gaccacattg tcttgatcca agaaccgttt
    10021 tagtttttct tttttcatgc cttcctcctg ctgctatcct agaatgtttg acgcaaactc
    10081 ataaagactc tctgctgctt cttctgctgt tacttcttca taaatgactt tttcaactaa
    10141 aagattgtat tggtcttgaa tttccggatt caaagcacta tcaaagacat tcacttcgcc
    10201 gttgtccata tcggaaatca tttctaaata atcaaatgtc gcttgcaaag ttgcatcatc
    10261 tgcacgagcg agctgttctc gaatattcga catgattggt acaccacgtt ctccttttaa
    10321 aatttcattg gcttcttcac tgttttgaaa gaaatcaata aacttagcag cttcttcttt
    10381 atgggctgag ctttttgcca tactcagcat ttgcgaagaa cgtaaaggga taccagattc
    10441 tccatctgct tttctgcgag gtggattaat caactgaatc tccatatctt caggagcccc
    10501 tgacaatatt tctgtcaact ggttacttga tagaaacacc attgccgctt ctctatccct
    10561 aaccgcctga aaagacagat ccgtcgtact tgcagctcca ccacctggtt ctggatgggc
    10621 ccctttgtct accagatttt tacgcagctg gaaaaaatct gttagatagt ctggattttc
    10681 aaatcccaag ctgttagata gatcttcccg attataaaaa ttcaatcctt tctcaacttg
    10741 tggtatttga ataatacagc cagctataaa atcttcgaat cttgaaatag catagattcc
    10801 taattcttca gtgatttgat agctgatttg ttcaaattca tcccatgtcc aatttgaagc
    10861 tggttcaggg atatttaatt gttcaaatat atttttattg taagcaatcg caaacgaatt
    10921 caccccgtta gacagtccaa ctaattgccc gtcgtaagtc gtcgttgcta aataggattc
    10981 atctgcatta gaaatatcaa ttatcccttg ctccacatat tcatctaatg gttcaatctg
    11041 attgatgtat tgaggaaatt gattgttcag ttggaaaatg tcccagatat catctgatgc
    11101 tgctagtgta cctaattttg tgtaatatcc ttcataatca taaaattcat aatcgatcgt
    11161 cacatgggga tttttttctt cataaagctc gattactttt actgttgcat cgtgtcggac
    11221 ttgcgaaccc caccaagcca tgttcagcgt gatttttttg tcatcttctt gcccacaagc
    11281 cgtcaagaag aataacataa cacttaaagc taagatgatt ctcattcttt tcttcattct
    11341 ctgcacctct acttccattg ttatttttca atatactgta tttttataca gaacatattt
    11401 attaaacgat ctttttcaag gggaataaaa accaagactt gccttatcgt tctgcctagg
    11461 atgaacaact caatactttc aagaaaaatt agatagtgct gattcttctt tcatcgctaa
    11521 aaacacttct tgcttcaatg atgggaggat aattacgcaa agaaaaaagg atatcagaca
    11581 actgcccaat atccttgaca tagctaaata agcatgcttc ttcgtgtgcg ctcttcccta
    11641 aaaaacagaa aaataactag taattctgta tgaaattagg tacaatatac acgtcgatgc
    11701 acaagcattg actatggcaa cttgattggt cagttcccgc tgacttttca agccccgaca
    11761 atttagctgc tactaaattg tcgggtgcct tttttagttt ttattcgttt ttcattctat
    11821 agaaagcgct tcaataaatc aagacatttt tttgttagaa tgctaataat aacaaaaggt
    11881 tttaactctt cttatgtaca ttacaggtaa aaaaacaaaa aaatttctct tttttttgaa
    11941 ttctgaacaa atagtactgg agtacaaaag acgaggaaga gccactatct gctcttcctc
    12001 gtcttttgat tctcacctgt tattcatgat tctttttctc agtttcccga tatttttcca
    12061 ttttacgact aaaaagctct ccgctacttg gtggcgtata tgtgatggca tttgctcctg
    12121 cttgaatggt ctcagcaata ctttcttccg ttggaccacc tgtcgcaatg ataggaagtt
    12181 caggatattt tttacgaaaa tggcgtactg cttctgcagt ttctttacct gcactgatat
    12241 tgataatcga aataccagct gctaactttt catctatttc tgtatatttc gacgttaccg
    12301 tactgataac tggaatatca actactcgac aaacttcttt tactgtctct aaaggcgtag
    12361 gaccgttcaa aacgacccct aacgaacctt gcgcttctgc gaacatgctc atgtaggaag
    12421 aacgagcacc ttgtgtcaaa ccaccaccta caccggagaa aactggaatg tcagctgcct
    12481 caataatgct tttagtaatc gctggatgag gtgtaaaagg atacacagca attactgcat
    12541 tcgcatttgt attacgtatg atcgcaatat ccgtagtaaa gatgatcgaa cggatcaatt
    12601 ttccaaatat cttgatcccg cttgcttcta aaatgatttc cggtactctt acgatatctt
    12661 ttctcaattc cgtcatgatt tctggcgtaa attgttctgc catcagccac ttctcctttg
    12721 ctatctccgg tcatactgat tcaattgata cgtctcgatg acctggatga ttttttgcgc
    12781 ataaccgggg tctgtcgcat aacctgcatc ttgcaatgct tgtgcggctt cctgatagtt
    12841 ggtggccgta atcacttttt catacagcgc aggattccaa tctactcctt ggacgaacag
    12901 ttgtgtatgg tcatccatgg actgttccca tgaatcatac actttgaagt ctccttggat
    12961 agttatccat tcttcgttaa caaattcctt tgtttccagg cttactttct tctgatcccc
    13021 ataagccttg atcccgaaaa gattattgta tttgctagcc aaggtacttt ttccccaatt
    13081 tgattctaat atagcttgtc ctaaaatgat actaggaagc actccataac catcttgtag
    13141 ttcttgggca tgcggaacaa gccgttcaat aaattgctga tgggattgcg cttcattttc
    13201 ttgctgatta tcagtaaaag gagaagacag attcttcaag gaaaaaacaa aggctaagag
    13261 tatcactgct acaccggcaa ccactgctgg ccaacggata ttcttgcgct tttgcttttt
    13321 ccactttttt ttcgccatga tcaattccta cttttcattt aattcattca gatattcttc
    13381 tagcgtcaat tggtgtttgg tgatatactc agcgttttct ttaccgacat aacgtaagtg
    13441 ccaaggttca tatgtgatct tagttatatc ttcgtgtcct tttggataac gaacgataaa
    13501 tccatactgc gcagcattat ctgcgatcca tttagctcca ggctgatctc cgtaacttgc
    13561 atccaataca gtagaaggat aactgttgta ccagttttga tcgacgacat cgactgccag
    13621 acctgtgtgg tgttcgctgt atcctggctc cgtaatcgtt tctttagtaa tctttgtcgc
    13681 ctcctcttct gatacacctt gacgactcat cacatcttgg acattttgtt cgaaaacttg
    13741 ttgttgggat gatacagacc ggtaagctga gaccatcact aatggaaatc cagctgcttc
    13801 agcagccttg gcaaatgctt catattctgg agcaatgcgt ttgtcgatca agtatccgtt
    13861 acttaatgat gccaattgag tagactcatc aatttctttt tctaatttgt gatctggacc
    13921 aaccaaaact agcttccaat cgtctgtttt aacatctggc aattgtttgg acgtacgatc
    13981 ttttgtttct ttgtccttta ccttgctgct ggttgtttga ctgttctttt ctttactagc
    14041 agttgttttg gtgcttgttc gttcgactgc ttgattgccg gcttgaacat gcgaatcatc
    14101 atcgctaaaa ataacatagc ccattgctgc gatcataatc acaactgctc caaatcctaa
    14161 aaatttattc acttgaaaaa tcctttcgtt gttcttcacg ttccccattc gcctcatgaa
    14221 cgtaagtttc ttccagattt tcattcaccc aatcgagaag ttcgattttt tctccttcga
    14281 tctgtttttg aacatctgct ggcagacgaa aattcaaaat atcatcatag ccccattcat
    14341 catattgaat acttactttc aagtcaaggt atctaccagc ctgctgatta tttttcaaag
    14401 ggatcagctc gacttttgct gctttgctgc taagccattt ttgcgcgatg cgtgttttga
    14461 tttgcttgta ctctgcaacg gcaacgtttc gtttttcggg aaaaataatc gtttgacgca
    14521 aaatcttttg cgttagcatc cattcataat cagtgaaaag attttttact ttctgcatcg
    14581 cttcagatgg cagatttttc tcgccatctt tccatttttg ccattgttct ggactgatca
    14641 gcaagtattc atctaaaaaa tttttttcag tttcaaactg ttctaataat acactcataa
    14701 taagatcaat atatacttga tgcacgttta tcccctcctt ttaactattc tacttaagtt
    14761 ttgatcaaaa agcacctctt aaatcgctgc tttcattttt tttaatgttt caagtcctct
    14821 cctctttatt cttcgtgatt tttgttataa taatagacag acaagaaact caatccaact
    14881 atgaaggagc gttttcagat gaaaaaatat caatggggaa tcgttggtct tggtaccatc
    14941 gcacatgagt ttgccgaaag cttcaatcaa gagacgagcg aactaacagc agttgcttcc
    15001 agaacttcag aaaaagcgga aaacttcgct caccgttaca atattccaaa agcttacgga
    15061 agctatcaag aaatgttaga cgatgctgaa atcgacatcg tgtacattgc tgtaccaaat
    15121 agacagcata tcgaccatat cttggcagca ttggaagcag gcaaacacgt tctatgtgaa
    15181 aaagccatca cgatgaataa aaaagaactg gctgacgcta tgcgcttagc agaagaaaag
    15241 aatttgatct tagcagaagc gatgacaatc ttcaacatgc cactttatca gcagttacgc
    15301 tcaataatgg atacaggaaa actgggggct ttaaaaatga tccaagcacc tttcggcagt
    15361 tacaaagaac cagaccctaa aaaccggttc ttcaatccag aattagctgg aggagctctg
    15421 ttggatatcg gtacgtatgc tgtatctttc gctcgtttct ttttgagctc tcaacctgaa
    15481 gtagttgcct ctacaatggt tccttttgaa acaggcgtgg acgaacaatc cgtcactatc
    15541 ttgcgtaaca aagaaaatga acttgctgct gtcagcttga cttttcaggc aaaaatgcca
    15601 aaagtcggta tcgttgcctt tgagaacggt tatgtgacga ttgctgatta cccgcgtgct
    15661 gatcgtgcgg agatcttatt tacagatggt acaaaggaat tcatcgaaag cgggaataca
    15721 tctgaagcaa tgaattacga aataaagaat atggtacaca tgattgaagg tgacttgccc
    15781 aatcgctctc tcttcttgac aaaagatgtc atcgaaatct tggatcaaat gcaagttcta
    15841 tggaaaaata tgtaatttta aaaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    15901 cagccaaaca aaaatagctc ctcctatttt tcgtttggct ggacttatta ccgcagagga
    15961 aagcttttga acaccgttta agaaagaggt tgtgagccta ccgttcagct ccgagtcaca
    16021 aggagcaatt tcaaaaaaca gcttctcatg tttgttgaat attgtgactt gtgaccgagg
    16081 agttgacagg agaacaccgt ttaaagaaga ggttgtgaaa tcagcttttt gacctgaata
    16141 ttagctgaat tcacaacctc tatttttgga ttttttgttg ggagaattct gccttgattt
    16201 caataaaact tttaaaagac tgcaaaaact ggaacaattc tgctcgatcc tcgaattcag
    16261 ggcgactagt cggcagaggc atttgccgat acaatacata aacttcttca atacgtgtta
    16321 aaatttgtct gccatcattt gactctgaaa atgtttctgc agtataaatc aataaaccat
    16381 agatatgttt tccataataa gctggttgct ggattctctc tagattttcc agcatgtctt
    16441 taatcacatt ggcctgtgct cttctcatag agaagtaagt ttcgtagtat agcggctgtc
    16501 tgagccagta gttttcttga tgttctctgg catcttcttg gctttcgcga atgaatgtca
    16561 gtaaatgttc gcatttgatc tgcacatctt ctttattttc tgataaaaga aattctgcca
    16621 tttgtctcaa aatatttcga aattcttttt cgatctgtat ttggttttcc ttcagctgct
    16681 tcgtacgatc tggcatgtaa acatttgcaa gcaaagcaaa tactaatcca atcgccatta
    16741 atcccgcttc attgccgatc agctgccaag acatgttttc ctccactaaa taatgagtga
    16801 ccaaaaccga atttactact atcccttcgg ttaatttgaa gcgagaggca agcgggatga
    16861 acaggaataa aaaaagactg aagccaaaaa tagtataccc aacagcaaaa aaactgaaca
    16921 gtgctgttgc cgtagctaat ataaaggctg cgattcgatt catgcccgta cgcaatgtag
    16981 agcgcttggt atttcctaca cttaataatg caatgatccc tgcagctggc caatacaaca
    17041 gcgataagct acccgctgtg accatcgaaa agaagacact gatcactgtt ttgactgttc
    17101 gcattcctat acgcatttac ttcccccttt tttattttaa ttatacttga ataatgaaaa
    17161 actagctatc agacaaaagt ttaggtatga tagtaaaaag accaaaaagg agaatctcta
    17221 tgcaatttgg ccgttttcga ctaggtatgc gtacgatcaa aagtgcctta gctgtttttt
    17281 tatgtatctt gttttttcat ttgactcaac gaggaatccc gatgattgct gcattatctg
    17341 ctgttttttc tttgcgtcag gacttgacta caaccgtttc ttttggaaag tctaggatca
    17401 tcggcaatac attaggtggt acattagcta tcatttattt ttttgtcaaa agctattttc
    17461 acaatgattt tttggtagaa ttgatcttgc ttcctattct agttattatt gtgatcgtcg
    17521 tgtcagatgg tattaacaat aattccggta tcatttccgc aatagctacg ttgttgctga
    17581 ttgcgttgag catccctcaa ggcgaatccg ctttgtatgc tatccaacgt gtactagata
    17641 cttttatagg gacattcatt gcaatcggta tcaatttctt tcttcgtcct cctgaaacag
    17701 aaaagaagga agaactatca gaagatttgg tcgaactcca aaagaaagaa cgattgctaa
    17761 gaacaaattt acaagagata gaagctaaga tccaaaaaca aaagaaaaat agcgaggact
    17821 aattttcttt tgcaataaaa taaaaaaatg gagagaagaa ctttaaaaag tcttcctcca
    17881 tttttgattg aacggtgaag tgtactactt tattcttcct tcatcgagtt ttgttctgcc
    17941 tcttttggcg cttcatacat atttacttgg ctagtagtcc cacgtgtcga caaattctct
    18001 acgatttcct tcaagtcaag tcctgttgtc gcttttagtg tttcttgagt agaagaaagc
    18061 aaatttgtgg cataatttgt cactcggttc gctccactgg tttctcctga actgcttcct
    18121 gtatcgacaa cagagatttt ttcgatattt cctagcggct gtgctgcttc gcgcatcaat
    18181 tgcggtagca tatcgataac catactcaag acagctgctt cgccatattc cttgaaggca
    18241 ttggcgatct tttgttttgc ttcggcttct gcttttcctt ttgctagtgc tgcttcggct
    18301 tctgcttgtc cagctaaacg tgtcttattg gcttccgctt ctgccagtgc ttccacacgg
    18361 aatcgttctg cttcggcttc agtcacttct ttgacttttt gagcttgtgc ttcttgttct
    18421 ttagcgtacc gatcggcatc tgcttttttc ttcacttccg aatcatattg cttttcacga
    18481 cgtgtgattt ctttttcttc cagttcgatt tgtttttgac gttctacgat tttaatctgc
    18541 atttcctgtt cgattacttg ttgttgtgct cgggcacttt ctaaattgta ggcttggtct
    18601 gcttttgctt tagcaacatc ttgttcttgt ttgtaggttg ctaatttcag ttctttttct
    18661 tttaatgatt ctgcgatctc tgtttgtcgc tgtaattcag ctgcttgtga ttctttttct
    18721 gcttgagctt ttttgatacg cgtttctttt agagcttccg cttctgctat atctgcatct
    18781 cttttgactt gggcgattct tggtttccct aaagagtcaa gataaccgtt tttgtctctg
    18841 acctctttga tagtgaaaga aacgatgatc aaccccattt ttgcaaggtc tacagaagcg
    18901 acttcttgta cgctttgact gaacttatcg cgattttgat agatttcttc tacagtcata
    18961 gaaccaagaa tcgagcgcaa gtgtccttct aatacttctc ttgcttcatt ttccagttct
    19021 tctcttgtct tgcctaaaaa ctgttctgct gctgtagcaa tctcttctac agatgaaccg
    19081 attttgatga tcgaagtacc atcacacatc accggtacgc cttgttccgt atacacttca
    19141 ggggtggaga cgtccaattt actcgataat agactgatcc gattcgaacg ttgaaaaact
    19201 ggcaagacga atgcgccacc tccacgcaca attttgattt tgttattgct ttcatcaatg
    19261 tgcacatttt tgcttcctaa ataacttcca ctgatgatca acgcttcatc cggttttgct
    19321 gtctggtact tcgctacaaa gacgatcagc aacatcagta aaatcaaagc tgcgattgcg
    19381 atcgcgataa aaacaactgg tatttccatt taacacacgc tcctttaagc ttttatcatg
    19441 ctttcataag gcatgacata acagattctt tccttgatct caatgatcaa tactttcgtt
    19501 ccccgctcag ctgtctgttc ttgggggctg taaaaacaag ctggacgact aatggaaccg
    19561 gtaacacttt tcaactgaat ttctcccata cctcgtacag ggatcggtgt gatcacaaca
    19621 gctacttgtc cttccagagt tttttcagaa gacgacatcg ttgattcggc atgtttcatc
    19681 ggcatcaata tatagaaatt aagcaaaaat accaacacta tcgatatgcc tccactagca
    19741 ataaataaaa ataaagatga ttgatctgtt aaacgctcac ctagaaaacc aaataagctt
    19801 gtgaaagtta accaagggat aataagcatc ggatcaagcg ggccatcaaa atcgaataca
    19861 tctccaaaaa taatcaatag gactgccaat cctgcacaaa tcagcaatgt ataccaataa
    19921 atcgtctcaa gtgcgattcc ccctatcata tacacgcctc cttccatttc tacgtctatc
    19981 ataccaatgt ttgaaaattc tgtatagctt cttttaccga ctcagtggtg ttttttatag
    20041 gaaggttgtg tttatcgttg acaatttgtt aaatttttgt tttcatgtta aaattttcac
    20101 aataaattct ttcgaggtag cctatgtttc ctttttttaa gaaaaaacca ttaaagctcc
    20161 agaacacgat catccttgtc gtttgtttct ccacattcgt ttctttgatt gtttctgcca
    20221 tattgatcag gaattttatt ctttcgatga accgatctag tgtgtcagat aaaatctcta
    20281 gcattgctaa aatcgttgcc aatgataaac tcatccaaaa aaagctgcag actggtgacc
    20341 cgacacatgc gattcaagga ataacaaatg aaatcatgat tgcatcgaat ttagatttta
    20401 tcgttgtcat ggaccctgaa ggcattcgat attctcacgc agacccaagt tacatcggtc
    20461 ttcctttctc aagtcatgaa gatgtgaaaa aagtgattgg aggacaagaa tttttctctg
    20521 aaaaagaagg aatgttaggg ttcggcttcc gctatttcgt ccctatttat tatgatcaag
    20581 agattattgg tgcagtttgt gttggcttaa caaacaacac gattgacaat caagtcaaag
    20641 atggacaaaa aatgattgtt ttttcattga ttattggact tagcgtaggc atcataggcg
    20701 ccgtttttct tgcccaaaaa atgaaaaaag tactgcttgg acttgaacca caagaaatag
    20761 cagaacagtt aagtgaaaaa acgattatcg aaaatgaagt agatgaagga atcttagcca
    20821 tctcttctga taaaactatc ctcttgatca atacacctgc aaaaaacatt ttctaccaaa
    20881 cagagcagct attggatttc gaagtaggaa aaaaatcagt gataatctat atgacgtttt
    20941 gtttaaagag acgattgagc atcaaacgac cgttcggact caatccatgt atttaaatgg
    21001 gatagaaact atttctagcg ttacacctct ttttagcaat gaccgttttt taggagcagt
    21061 cgcaacttta cgaaatcgat cagaagtaat gaaattaatg gaggagcttg gggggacgaa
    21121 acaatatata aactccatga gagctcaaac ccatgaattc atgaataaac ttcatgtcat
    21181 ttctggtttg attgatttga agaagtatga agaagtcacc cattttatcc agcagctaaa
    21241 tcaaaattat aaaaaagaga ttggccaagt ctcaagtctg atcaaagtac ctgcaattgc
    21301 cggtttttta ttaggcaaaa taaacgaagc caaagaacaa tctgttgaaa tgctgttaga
    21361 aagcgattct tttatcccta aaatggagat ggatgactcc atccattcac ttttgcaagt
    21421 gttagggaac ttacttgata atgcaaaaga agccgtgcta caaaaacatg aaagaaacgg
    21481 acaggtccgg attcatttaa actatgacac agaagctgaa gtattcatcg tgaccgtctg
    21541 cgatgacggt gtcgggatat cagaagaaat ctcagaaaaa atatttacgc taggtttttc
    21601 gacgaaggga atcaatcgtg ggtttggatt gaatctcatc cattcaatcg tcaaacgcca
    21661 cggtggattt ttagaatttt ccaaacaaac aaatggcggt acaatcgtct atctagaatt
    21721 accaaaaaag gaagatcaaa atgatgacaa atgtcctgat aatcgaagat gatccaatgg
    21781 ttgcagaatt gaatcgtact tttgtggaaa gcatccctaa tatgaacgta atcgaaagtt
    21841 tcagaaatgt agaagaagga aaaaaatatt tcagacaaaa aaacgcagat ctgctgctgc
    21901 ttgatgtata catgcctggc gaaagtggta tccatttttt atcttggctt agacagcaag
    21961 gaaatgatat cccagtgatt ttgatcactg ctgcagatga tatcgaaacc ataaagaatg
    22021 tgatccgcta cggtgcctta gattatttga tcaaaccgtt cactttgcaa cgttttcagc
    22081 tggcgatcga aaagtatctt gctttctctc agacaatcca aacgactgaa aaagccgatc
    22141 agaaattgat cgatcaagta ttcaaccaca ccactcccat tcacgaaata ccatcattca
    22201 tcgatttgcc taaaggtctg tcaaaactga ctttaaaaaa aataagtgca tttattctaa
    22261 aagaagatac tattttttca acaaaagaac tggctcaaaa aagctcactg tcacgtatat
    22321 ccgtcaagaa atatttgaat tttttagtag aacaagatat attgaacgaa agaatcaaat
    22381 atttggaaat cggccggccg ctaacctgtt atgaattgac aaagaatggg cggaaaaagc
    22441 tcacagatta ctgttgagtc atcgaattat cccatacaaa atagtccaag tatacagaag
    22501 taacaactgt ttacttggac tatttttaaa cattttattg acgaatatcc atgttttctt
    22561 atcttaattg acgggcttta ggaagagata cctcattttt cgtttttcca gtttctatta
    22621 tttcacgaaa gttctcgaaa cttgtcgaaa tcatatttga taatgcctca tctgtatttg
    22681 aacctacatg cggggttaca agaactctag ggtacatttc tactaattgc tggatagcag
    22741 ggtctggaat cgtctcccat ggcttgaagg atcggaaaaa aacctctgtt tcatttgcaa
    22801 aaacatcggt tgcaaaacca gctaaatgat tgcttttcaa agctcttaaa atagcttcat
    22861 tatcttgaag ctctccacgg gctgtattaa tcaagatgct accttttttc atttgagcta
    22921 aaaatccttc attgatcatt tgatcgtttt cacctgggat atgaggtaca tgaatactga
    22981 caatatcgct tttggctaat aactcttcaa gcgagtggaa cgtcaatact tttttagcag
    23041 cttctgtctg atgaatatcg tagccgatca ctttggctcc tagtccctta aacaaaacag
    23101 cttccgtcaa ccctatcctg cctgttccta tgatccctac tgtgcaattt ctgatttcac
    23161 gactaaacat ttgctcatcc actcgaaaat ctttgaaaga tgttcggatc gtcgtatacg
    23221 cagtatttct caaaagcatc atggcaaaag tcaaagataa ctctgctatc gcatttggcg
    23281 aatacgatgg tacacgggct actgtcatac caaaatcagc tgccgcttgt agatcgatat
    23341 gattgactcc cacagtccgt gtaaaaacat aacgaatccc atattcctgc atttttgcaa
    23401 gattggtctc atctgctata caatttcccc tcaacaatac agcatcatgg tcctttgctg
    23461 acacgatatt gtcatgtgtc aacagttctt cataaagact gagatcataa tggtaaatat
    23521 tcaatttttc aaaaaaatct atctcgttcg gacgaacacc gtaacatgcg attctcatac
    23581 tttcttcctc catttcttat gaaaataaac ctatgccatg aatcatttgt aatacaacga
    23641 tccatacagg tgtcagtacc acagcaaaaa tagttgacag taaggatgca ttggaggcta
    23701 aaagtgcctc cctatcaaaa ctgatagcat aggctgctgc cactgttgca gctggtgtag
    23761 ccatcatgat aacgattgtt gccagtgctt caaaattgac tggtagaata ttcgttgccg
    23821 tcaaaactgc tagaatcacg atattcaatg caggtacaaa taccactttg actagactgt
    23881 aataccatga agttttttca gatgcagctt ttttgaagct tacttcacct aatgttgcgc
    23941 cgattgctag ccacgccaat ggtgaactta aaccagctaa ataatccatc ggctgaaata
    24001 gccacggtgc agtctggtca attcgcaaaa acgctacttg gcttatctct ccttctgcac
    24061 ctgtaactga cacttgtggc agataccctt ggaacagcca aatgaaaagg ccggcaaaag
    24121 tagcgataat gattggattg acgaacatcg ctttgatatt tttagcttcc atcttcaagc
    24181 cactcatttt tatgtatcca taagaataca aaaagattcg gtagccaata ttgaaaattg
    24241 aggaatacat cacgccgaca ggaccataaa tcgcactgac gataggcgtt ccgaaaaagg
    24301 tcgtagaacc aaagatcgtc aggacacgca acgttgtttg cttatcccct ttatagctta
    24361 agaaaaaagg ttttgaaact agaataagaa taatataaat aactaatccc caaatcaaga
    24421 cattgattcc ttgcatcagt aattctgtat tgatatcttg catgaaagct cgaaatgcca
    24481 gtgctggcaa agccactgtt aagacgactt tcgatagaat ttttccaaca gattcagaaa
    24541 aaatcccctt ttttctacaa acaaacccta ataatataat aaaaactgtt gaagtgattg
    24601 cactgacaat cgacatatct gtcaaagtcg atcgaaaaat gtcaaataag ttcatctttc
    24661 tcctcctaat tatgttcatt tattcacaat aaaaaaagct tttattttgc taatacgtat
    24721 taacattttt aattttatct ttacattgtt acttattgaa caataggaaa ttaaagaaac
    24781 ttttgaaact tatgtaaagt gatttttact agaacacaa
//
LOCUS       DAYYGY010000041        24356 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000041 DAYYGY010000000
VERSION     DAYYGY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.042"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..628)
                     /locus_tag="ACHA52_001570"
     CDS             complement(176..628)
                     /locus_tag="ACHA52_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940272.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(635..1183)
                     /locus_tag="ACHA52_001571"
     CDS             complement(635..1183)
                     /locus_tag="ACHA52_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8940273.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1228..1560)
                     /locus_tag="ACHA52_001572"
     CDS             complement(1228..1560)
                     /locus_tag="ACHA52_001572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940274.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1574..1831)
                     /locus_tag="ACHA52_001573"
     CDS             complement(1574..1831)
                     /locus_tag="ACHA52_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940275.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2026..2580)
                     /locus_tag="ACHA52_001574"
     CDS             complement(2026..2580)
                     /locus_tag="ACHA52_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8940276.1"
                     /translation="MTRNVKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIE
                     AVKETSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQA
                     VRDYMTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVL
                     LQLKVDQEAQKIDAIKIYYVRSAY"
     gene            complement(2589..3680)
                     /locus_tag="ACHA52_001575"
     CDS             complement(2589..3680)
                     /locus_tag="ACHA52_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8940277.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3688..5898)
                     /locus_tag="ACHA52_001576"
     CDS             complement(3688..5898)
                     /locus_tag="ACHA52_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8940278.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5914..8442)
                     /locus_tag="ACHA52_001577"
     CDS             complement(5914..8442)
                     /locus_tag="ACHA52_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8940279.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8494..8904)
                     /locus_tag="ACHA52_001578"
     CDS             complement(8494..8904)
                     /locus_tag="ACHA52_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8940280.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8917..9135)
                     /locus_tag="ACHA52_001579"
     CDS             complement(8917..9135)
                     /locus_tag="ACHA52_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8940281.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(9132..10124)
                     /locus_tag="ACHA52_001580"
     CDS             complement(9132..10124)
                     /locus_tag="ACHA52_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8940282.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(10203..11114)
                     /locus_tag="ACHA52_001581"
     CDS             complement(10203..11114)
                     /locus_tag="ACHA52_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8940283.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(11133..11477)
                     /locus_tag="ACHA52_001582"
     CDS             complement(11133..11477)
                     /locus_tag="ACHA52_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940284.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11497..11775)
                     /locus_tag="ACHA52_001583"
     CDS             complement(11497..11775)
                     /locus_tag="ACHA52_001583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940285.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(12134..12634)
                     /locus_tag="ACHA52_001584"
     CDS             complement(12134..12634)
                     /locus_tag="ACHA52_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940286.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12635..13180)
                     /locus_tag="ACHA52_001585"
     CDS             complement(12635..13180)
                     /locus_tag="ACHA52_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940287.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(13177..14433)
                     /locus_tag="ACHA52_001586"
     CDS             complement(13177..14433)
                     /locus_tag="ACHA52_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8940288.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14794..15105)
                     /locus_tag="ACHA52_001587"
     CDS             complement(14794..15105)
                     /locus_tag="ACHA52_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940289.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(15128..16603)
                     /locus_tag="ACHA52_001588"
     CDS             complement(15128..16603)
                     /locus_tag="ACHA52_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8940290.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(17052..17504)
                     /locus_tag="ACHA52_001589"
     CDS             complement(17052..17504)
                     /locus_tag="ACHA52_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940291.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17506..17871)
                     /locus_tag="ACHA52_001590"
     CDS             complement(17506..17871)
                     /locus_tag="ACHA52_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940292.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17970..22253)
                     /locus_tag="ACHA52_001591"
     CDS             complement(17970..22253)
                     /locus_tag="ACHA52_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8940293.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22400..22639)
                     /locus_tag="ACHA52_001592"
     CDS             complement(22400..22639)
                     /locus_tag="ACHA52_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940294.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22729..22971)
                     /locus_tag="ACHA52_001593"
                     /pseudo
     CDS             complement(22729..22971)
                     /locus_tag="ACHA52_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23084..23320)
                     /locus_tag="ACHA52_001594"
     CDS             complement(23084..23320)
                     /locus_tag="ACHA52_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940295.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         7228 a         5250 c         3527 g         8351 t
ORIGIN      
        1 agtagttatt ttgaacttta acttttaaaa actttaaaaa taatcttatt tgttcttcta
       61 aacaatcatc tatactcata taatcactcc actaattaat ctccccaaaa agacacagca
      121 ttaagagata taccaacaca acagtgcaaa taactctaca tcttccttcg caaccttatt
      181 tcaataaacc ataaaaatta ttttttaata gaaactgaaa attaaaacta gaaaaagtag
      241 ttttaccgaa tgtaagagca tcatcaccat caatatagaa atacattctt ttccagttac
      301 atttaatttt taaacaaaat tctccccaaa aaggaacttt ttctagtgaa aacttggtaa
      361 tctctttgta attaattctt gttaaagaat aattaaacac ttcattttta cttgttattt
      421 tttcattgac tataactagt tcatctggta aaaaaattaa ccaacaaaca tcgacaaaca
      481 gtaaatgata taatccattt ccacctccaa gcatattcaa tactggtgaa catgccttag
      541 aaacacgaat atagttatgg tttaaataga tattttcttt aaacattgct tgtttaaaag
      601 ccatatgctc ttgttctaaa cttttcataa acttttactc taatgcacag ctagcagcag
      661 ctgctgcaat tcctgctaca atatttacct tagctaactc tttagctgca gcttttccta
      721 attgtttttc tgcaattttt aataataaat ccgcagcttt ttcaaaatta aaagctttta
      781 atgcttcaat aaaatctccc tgcgtaccta tagcccaagc cactcctcct ccaggaagag
      841 aattaatggc tacacatcca gcatatcgtt tccaatcaac tttctccatt ctaggaacaa
      901 taatatgttc ataaaagact cttgctgctc ttccttgttc gttatcttga ctgattcgtt
      961 cacgaatagc accaaaatcc tttatatggt atacaccatg ttcgtctaaa aaaccaactg
     1021 tatcaaaata aaaagttaat tcatctacta atttattctt tatttctaaa tcaactgtat
     1081 tcactttttc tgtggcatac acacttgttc cagtactaac aaaagtactt aaacctaata
     1141 agaaaacacc taaaacacca aatattttaa ttattctttt cataatttct cctccataaa
     1201 aaataagtta ctttttaaat atcttgttta tgaattcatt ccccaaaggc cttttaagat
     1261 tgatacagca gtccgttcac ttcttttaga cttgtcatac aagtctttta aatgatccca
     1321 tatcagacta gtttgtctat ctagcttcaa tccattaaac gctaatgaac ggttcatttt
     1381 ttctattact ttagataaag ctacagaaat ttttatacct tcacaacgcg catcttggat
     1441 ttcaataata gtatcatcaa ttagtttttg aatttcttgc ctatcaacat attcaatctt
     1501 agaaagagaa tcttttaaag ctactaatga ttcaacgatt tcttttttct cttcagacat
     1561 tttcttcctc cttctagtga cccgtaaagc catgtagagc agagtccatt gaaccagtaa
     1621 ttacactatc tacaacgcca ttccaattta ctctacattt tccatttttc agatttgtcg
     1681 gtccatacgg atagcctttt tgacattcaa tgttcggatt ataccagata ggattatacc
     1741 attcagccgc agaagcagat tcagtcgata caaacaatcc cacaccaaca aatccaatac
     1801 acatcacact atagccaatt agttttttca tctcgtatcc atctcctaaa aaatagaata
     1861 gttcttatag ttattcataa gacatatttt ttccgttttt gttttatcct ttctgtccta
     1921 taacaacttt tttcaagtaa agaatgacat agtttttctt aaaaaaacag tctgtttttt
     1981 tctatttctt cggtgtataa taaatatgag tgttcatttt ttattttaat acgcactccg
     2041 tacataataa atcttgatcg catcaatctt ttgagcttct tgatctactt tcaattgaag
     2101 taatacaaat tgagtaacct tattcattgt ttcttctcca aataacaaat attgatccgg
     2161 attctctata tcttgtgtca cctggtaaag tttcccagtt gttttcattt gtacatgcgg
     2221 gtctacatca atactattgt ctttaataca acgatctgtc atatagtccc tcacagcttg
     2281 attgcgttta taaacatctg tgaaattact ccattttcta gcaaaatctt ttaagaaatt
     2341 ttttgtcatt tcatctattt gcatttgttg cgctgtctcg tcttctaatg aaccaatttg
     2401 actgttactt tcagtacttt gaagagttcc acttgtttct tttactgctt cgattgtttc
     2461 ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac aaccagtaag
     2521 acctatcaaa cataaccaac taaatactaa aattatccct tttttcacgt ttctagtcac
     2581 tgcctttctt atttcacctt acgaaccaga ctacgaattt ttgtttgatt gtacgtgcgt
     2641 tgataccgag ccataatccg tccttgctcg gcattctgtt ccacactaga gaaatgttgt
     2701 ccgccatatg aaacatctgt tataattccg gtgtgaccat agattccagg agacaatgca
     2761 ccacctgctt gccaattcac cacatctcct gcttgtaaat cacttggttt tgggttcatg
     2821 attacttgcc aaccataggt tgtccagtta taatcttccc ctattaacat cgcaaacatg
     2881 tgtccagaac ccattaactg tgggccacct aattgagata cataccatgc cgtaccacca
     2941 taacattccc cattatttac ggatcgtcca atgatccgat ccaaaacctt cgcattgcct
     3001 ttactttttc ctgcaataag cgaagttttt tttgttggta ggctattcca atgcttgatc
     3061 gcaaaagtat catattccgt taagttatag gacgttatga tactcattgc tcctcgataa
     3121 tacggactcc cttcggtaat acctgctgcc gcatagcctc cacgacctag aatcaataaa
     3181 caacttccag gatctttatt gcccacacat tgattaaagt tgctttggct cgccaccata
     3241 aagccataat agtcataaca agcttttaag ttcggaaact tcatatagaa accaccttca
     3301 gaaccaccaa ttccacgtgc agaaccttgt gggtagggac aaccaggaaa ccaggtaatc
     3361 ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc cataagctga ttcatagcct
     3421 aattgagaaa gaatcacact cggtaataac tgatacttta cagctgcacc aactgctgct
     3481 tgcgcatgtt catctgtgat tggaccacgt cctttaatct ctgaaaaaat cggtaatcct
     3541 tcggtatatt ccccattaaa agcgacagct agacctgtag cttctcctcc atgctcagaa
     3601 agttctcctg aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa aagaagacaa
     3661 cctagacacc ctttcttcat ttcttcctca tcctttcttc gataatggtt tttggactgt
     3721 tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg gcagtgtttt
     3781 aggatccttt tctatttttt ccgattgctc tgttggctta tctaaagaag aaaatgcagg
     3841 ttgtaacgat tttggtgcgg aaatcgacgg tttactttca gacgaacgtt gccccggttg
     3901 ttgcacagaa cgatttcctg atagagtggc taccgacgaa ttaggaacag agtattggtt
     3961 caataactgc tgcattgctg gataactccc atttttcgcc tgatttttca cttggcgaat
     4021 ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg ggtacttttc cagaaggcaa
     4081 cggaatcgat tgaccaacaa aagcgatacc tttatttaac aatggttttc tttgattatt
     4141 cccttgttgc ttactcaatt gtgccaatga ttgtacctgt tgttttggtc ttactgaatt
     4201 catggacgaa tttggaatca aaccatttga aagaccagaa ttccactctg ttttcgattt
     4261 ggtagaagac atttcaggct ctaccccatg ataacgaaca tgacgaccac tataaggcaa
     4321 ggcttgattt gaacgcccac ctagttcaaa gggattaacc acttgcctcc cttttaccga
     4381 ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg cagaagcgag
     4441 tggattcgtt gctcgataca cattccctgc taatcctgca cctttgattg ccaaattttg
     4501 tccagcagtt gatagttgac caggcagttg tttcatttga gacaaagaat gacttgctat
     4561 gtttttcgtg ttgtttatag tttgatctgc agctcctaac cctagtttct ttaataaatc
     4621 tttccgtttt acaatgagta aaattgatag gagtgctttg acaaacatat ttcctaaaaa
     4681 accaaaaaca gtattcattg gaattaaatc gtctactatg ttatagatca aaaatgccac
     4741 caaaataatc acactgtaaa aagatttttg aaacatcaag cccaaaaagg actttaaggt
     4801 attcatgagc atataatcaa atactggaac aaaagaagcg agtaatgaaa aaggaatcac
     4861 aagtaataaa cacaacacca acatttgaaa gataaaacga atcatgccaa tcactacaag
     4921 tggtaatcca atacttagat taagcaatgg caccatagaa ccaacgactg ctttataaac
     4981 agctttagtt ggttgaatat agacacgata agccttcttt ttccctctat cttctcctgc
     5041 agcagtggtc acttttgtct caatatcaga taattcttca cttgacgctt ccttggctag
     5101 aaattcttgt ggttcaatct ctgcttgata caattcttct aaatttgttg tcccataatt
     5161 taacaatata tacggatcaa caacagccgc tttataataa agttggcgca ctttttgaat
     5221 ggcttccttt tgtgtcgttg gcactaaaac accattgaca tcttttgtcg ccaattcatc
     5281 tagactattt gtagcagaaa aaataaagcc ttctacctcc acggacaaat catttaaccc
     5341 tttggtaaaa gcttctcctt tactaggtac gttactacca tagccaaacc aaaccattga
     5401 aaaaatgaca acgaagagaa atcgaataaa caccattttt gcgtaatgtt tccctctaaa
     5461 tatacgaaga gtaaatagat ataacacata caagacgaca atccccgcac caataggatc
     5521 gtatattttt gaataaatag tatccgaaaa agtgaaaaac tggtgaatta actcgttcat
     5581 tgcagctcct tcatagagct tctcaatgac aaaatcaagg ccttgccaaa tcaatttact
     5641 taaactaaag aacatattcg tcatcatttt tagaaaatct gtgccatttg ccccaaataa
     5701 cattttgcct aatgaaaatt cactatcaga taaataagaa gaaaaggtat caatatgaaa
     5761 tttcccaatc tcatagatcg attgatccac ctgcttttct gccgcataca ccaatccacc
     5821 aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt tcttttcctt
     5881 atgctttata taagccattg tttcacctca tttctattgc atgattaacg tatcctgttc
     5941 ttctacttga gagtgacttg ttttttccgt tgtttgtaat gcagcggtca tttcttcaaa
     6001 caaactgtgt acattgagtt ttcctacacg tccataggga tctaacatcc aacactgtcc
     6061 tttaatcatg tggctcattt cagctaagtt cgcttcattt acttccaaac gtaaatgatt
     6121 caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa aattcccttt
     6181 ttctttatct gaacctaagt caccaataaa ttgtgtatct aacactaatt gattcatctg
     6241 ggaacgcccc acacgtgcca ttgctgcaat aatactacgt cctgcgttcg aattattaaa
     6301 tacccatgct tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt ttccaaacat
     6361 ttcacaaaaa cgacctaaac taatcatcaa acacaaagac tttcgatccg catctgtata
     6421 tagttctggt cggacattgt catttggtaa tttcaaatct tggatctcta aaatcgttgt
     6481 tttggcatta aagtctaatc ccgggttttc tccataagaa aatcctaaac gtaaaatcga
     6541 atcttctacc gtcaagcgta aaaactcacc atattctcga acctcttttt gttccatttg
     6601 ttctaaatag tcgaccacat gcatcaagcc aacttgttgt ccggcttctt tttgaactaa
     6661 aacttcttgg attcccttta ataccgcccc ttttaaaagg tgattggtac ctaacggact
     6721 taactcatca atcatatcct gggctacttg tttcgcatct actcgggata aacaaacaat
     6781 tggatctaat acaccataat tacggggatt tgtagcatct aacgtgacat aactcatttt
     6841 ttgcaaatgt tctacaaaca aagggtaatg tttctgataa tacgggtctt gaatgagttt
     6901 cgtaaaccat ttgcgctttt ctcctttggg atccacaaat aaactttgta cattcataaa
     6961 cgaactatac aaaaagagca agccaactaa aaaggtcttc cctttaccgg tttttccagt
     7021 tactgctaca tgaggcgcat caaacaacgc acctttgatt ccttccgcaa tggctagaag
     7081 attcaaaaag acaaaacgat ttgacgcata aattaattct tctaatggtg ttttttctcc
     7141 tcgagaacca ccatccccta atgtatcttg ccgaccaata taccagccgg attccatacc
     7201 taattctgat actgctgaaa acaaccattc gccaactgct tctacagttg ttcggtgctt
     7261 ccaatgtaac tgccgtccca atggttgccc taataaacat ttatagaaca attctcgttg
     7321 atcatttttt gcacggtgga tatggataat cgtatgaagt tgttgaataa ccgttgcact
     7381 attttgacga acttcttcaa ccgtttgacc gtatacaata aaacatgcga tccaatcgac
     7441 gacaggttgc ccattatcta ctcgttgctt taaatgtttt actaatcctc gaacagttaa
     7501 taagcgttca ctaggttctt catcattttg aaccaattcc ttatgctctg ttttgaaccg
     7561 ttccgatagc caattcagtt ttaaaccaat acctccctgt cctttcaagc gtggatagtg
     7621 aattttcatt cgcaattctg ctgggaaaga aagatcctga gcaaattgaa atagatcaca
     7681 atttctcata ttttcaggtg tttccccaat aggcaacaat gctaaataac tactccctaa
     7741 ctcatcttct aattgaagaa ggcctttatg ttccttcggt atacgtacat acgttccttg
     7801 attgtctttc agtacttccc aattattttg ttcttctact ttgtgagcca ttcctcgaat
     7861 atattgcaga cgattgagat acaacagctc tttttttact aagggacgaa tagaaaagct
     7921 ataaagtaat tgtgtcactt cttttacata aggatcactt tcagaaaaca aagtttccaa
     7981 atcatctact tctaatccaa ataagcgtgc taaacttcct gctaaatcta accagattag
     8041 atttattaca tcatgaaaac tctccaccgt tgctttttct ttgactttga ttcctaaaat
     8101 aaatgaagga cgatagactt gaccttctcg ttccaacaat gtcactgttt cttttgcata
     8161 atacgcacca attgcttgac tatcgggaga aaatcccttt cctaatgtgt caaacttcgt
     8221 ccgtaaattc attgttcgtg gatacatcac caactccaca tcttcataat ctgctaaaag
     8281 caacagtaaa cgagccatca tttcttttat ttcctgtgtc ttttttggtg tgttttttga
     8341 aattgttcgt ggaaaaacat gatagtaggc ccagatacaa tcctctccat ttaacaaaca
     8401 atttttcttg aatgctttta ctggttcttt tacaagcacc aatgaatttc acctcccaat
     8461 aaaaaaggaa caaccagttg attgtccctt tttctaatcc tctaaataaa catacttatt
     8521 cacaaaaagt aattcttcca atgcattgtt tgataccttg tcccagttat aatacacaca
     8581 atcaccattc atcatacgaa tcatataaca aaaccaatcc cataaataaa aatgcacttt
     8641 ttttccatca ggttttagtt ttgccagtgc taacacacat aaatagggga gtcctatata
     8701 aaaaacaact cctgctggtg gaaaaaaaga aatgacccaa ccaataaatg aacgtaaaaa
     8761 taaatattca aagattacga ccaacaaaaa agttgctgca ttgactaatg caactggaaa
     8821 tggaagacgc caattgcttg taatctgttg tacgcgataa ggttccgcat aaacaatcga
     8881 ataatcataa ggttcccaat ccacttcact atcctcctaa tccataaata atttttcaat
     8941 gattttacca aacgcatcaa atacacgtgt tggatcattt aagaagaaat aaactgcggc
     9001 acctgctgcg atagatatta atgcatttcc tacttttgat ttcataaaat ttcgcaatcc
     9061 cagataaaca accaccatta ttaaaaaata gccaccttgc gttaatataa aatctacaat
     9121 tcccttcagt gtcatttccg ttcttctcct ttcctatcca accaattatg tgttaatttt
     9181 tcaacataat actgatttgt ttttttcgta atgacaatcg tcatttgttc tttttgtaaa
     9241 agtgacgtat caccatctcg aaagacgacc gttgctttta caataaaagg ttttttcgtt
     9301 tctttataac gcaatacttt catctctact tgatcaactt tagctgtttg attgagtcct
     9361 tcaggttctt tcatcatgta tgacatatct tttgcattgt ttttcgcata cttatctaag
     9421 aaatcttcca caaatccttc aacatctttc gcttccgacg catcaaccgt ttcgtataat
     9481 gtgtcatcat aagtaatcat tcgttgtttt tctgtattct ttggtagtgc cgtaaaataa
     9541 ggataagcaa taatacgaca gcccttgttt gaaatagaaa agggaagatt gagtacttgt
     9601 gtgattgtca cacttgtttc tttcttactc tctggattca tcgagcgaat atcataagtt
     9661 actttataac tagcagttgg aatggtttca atttctttta atccaataaa cgaagcagca
     9721 gttaattttc gaacacctga aacacttcct ggttcttgaa taaagttttg tgcataaaac
     9781 tcttttttta atgtttcttg acgttgttgt tcttgttcat gattatcgaa tgtattcata
     9841 tacaaagcaa caaaacgatt cataaaggat tcaatttcag gtgtgtacgt taattctgac
     9901 cgactttgta cttctttctt ttgtaattgt tggatttcat ctttcaaaaa cgacacttga
     9961 accttcgttt tcatggattg aaaaaatgcc aagccccctg aaaaacaaag aaataaaacc
    10021 aaacaccaaa tccatttcac tgcttttttt gaatggattt tcttttgctt cggtactttc
    10081 tctgtataaa ctttttcttt ccgccgtagt tgtcgttttt tcataagcta ctccttctag
    10141 tatgttttta ctagtacgta cgattgacaa cgataagctt gtttttctga aaaatagtcc
    10201 attcaattac cctcattttc tattcctttt ctctttcttt tatatccaac aaatccagca
    10261 atacttgtca acactcctcc tagaacagaa agtgcaacat gtatttcttc accagtcttt
    10321 ggtaagactt ttgtttttcc atctgctgtt ttgatttcaa tattgccact tggaagtttc
    10381 tttacaggaa tcgacaaatc ctttaccaaa ttcccttcac tatcttggat taatggaata
    10441 ccatctttta caccaacaat tgtttgatca cctgctactt ttaatggggt ggttggtgtt
    10501 ggaatatggg tcaattcctc tacattacta gtgacaatcg gtacactcat tccttgagaa
    10561 gtattcgtcg taatttcacc gcttggggtt accgatactg tgacttcttt tgctggaaca
    10621 ataggtttat tttcttttgg aattgcttct gatttttcaa caggttgttt cggttcttct
    10681 ttttgttctt cactggtaga cgacatcgtt ggctgttccg tacttgacga atcagtattc
    10741 ttttcagaag aactggttgt tcctgattgt tccggagttg tgactatact tgaatccgtt
    10801 gttgttgaac tatgcacacc ttcttcttcg atcgaatcag tcggtaaagt tgattcagga
    10861 aactctggtt ctggtgtcac tggctctttt tcaggtttct ctgactgcaa ttgttcaggt
    10921 tctgctggtt tttccgttgt tcctccattt agctctgtct caacatctgg taatccttcc
    10981 cctggcaatg tttctacaat ttctgactta ggtgtttcta cagtttcatc tgcataaacc
    11041 aatgaactcc ctaattgaaa cgaaaataat gaaattccta gtgttgttac gactgctaat
    11101 gttgtttttt tcatgattat tcctccaatt ttttatgcaa ttcgtgtttt gttcatagac
    11161 gccgcactaa tattatctga acgataaaag aactcccgtc cgttttcttc taacccaaca
    11221 tatggattat ttttgaataa ttgaatcaat agttcactgg tgttgtattt tggatagttt
    11281 gcaaaaaaat aatactgtgc ttgtcgccca tccgaaaatt caacaagtaa ttcataacga
    11341 taccgcttca atccttttcg ttgttttaaa ggatctctat accaacttgt ccatcgttcc
    11401 attcgtggat agagatagac acgatacaca tagaacaatg ccataccaca taccaccaaa
    11461 aataataatc gccacataaa aattccccct tttcttctac ctttcttgaa tcatcgaaca
    11521 ttcataccct ctatagagat ttgaaaaatc catcatcaat tgttcatata aacgttgttg
    11581 caaatagcga ttgtttcgaa tacataattc ttcatagcca tcaatcactt gatcttcttt
    11641 ttcatagcat cttgtagctc tatgacgctc agcttgcatg acataaccaa actctgcacc
    11701 atatcgttct actgttaata actgttgact acgcaataat gcaatataac aatcacaaaa
    11761 atgttcaacc gacatttgat ttttctcacg tttgtgaaat aggtattctt tagaaagcac
    11821 attattcgtt aaacaatctg ataaatagcg ataatctggc ggtcggttat tttcatctgt
    11881 gaatttttcc cataataaac tgatttttat tcgttcagcg gctagatagt cttgttcgcg
    11941 tggataaaat tcatcaatcg cacaaagttt tttatcacct gaataaacaa atccatataa
    12001 tgcgaaacga actcttcctt ttcaattggc tctcctaaaa attccagtga cataccatct
    12061 tctccttcct aaaaaaaata aaaaagcaga aaccgatttg gcttctactt ttctttcctt
    12121 ttatagctct tttttacgga agaatccgca aatgacattt tttcaaaaaa tcaatcggca
    12181 caagttcacg aaaacttgga gcaattttgg atagaatcac ttccccatct tgtaaaatac
    12241 aatcataaaa atcatcagtt acttgatact tacgcttctt acaataattg ataaaatagt
    12301 agcgatcatt ttctatttcc attcgttgtt ctactaagcg catacgaaca tcatcataga
    12361 aataagccac aaatcgccct tcaagttcat gtttttcaat gggatactct tctggtaaca
    12421 atctattttt tcgtcgttta tacattgctg gataccttag aagaagttca tctaaacgtc
    12481 tgtcagtata tggtaactga cgaatcaacg attctaatcg ttctttatac agatagaata
    12541 attggcgcaa gcaatagtgt accgcctctg gattttctaa tactgttgga tcacgtgacc
    12601 aatacgcacc aacataaatc ataatttttt tcatttagac aatcacacca gcaatactaa
    12661 tgaccgatcc acaataggta gaacaagtgg catagcgacc aatttcctct aattctatct
    12721 caatctcttc taaagaaaga tatgtctgtt cttctgttgg ctggcgactc atttcccaag
    12781 tgacagtcac caatgaaatt tcttccataa gaggtttttc gctgctaagt gctgccacac
    12841 agttttcata tatcttcatt ttatcgcagc cttttttaac cgctaaaatc gcaggtattg
    12901 gcaatgaaaa tcgataaaag cgaatgaatt ctagccttcc tttccttcgg ctaaattcca
    12961 actcttcgag ttcttgttcg tatcccttac gaacttctaa taagcgtgca atttcttgcc
    13021 agttctcttt ttcctgataa cgtccagctt ttaccgcttg ttcaatataa ggaactcgaa
    13081 aacgaaaata agacaaatca accatctttt catttaatgc ttcgattgca gcctttaaat
    13141 ccaacaggcg cagccattct gttgtcgtat atgcattcat gcaattttcc ctcctgacgt
    13201 tcccatccat tctagatagt tttgttgctc gtcatttagt tcggcattat caatcccttc
    13261 ttgtacaaag tctcttccta aaatacgatc tgcttcttgt aacaacttta atgtcggagc
    13321 tacttgtttc aaaaaccagc gttctgaacg atctaatgag aatttttctg gctttactgt
    13381 aaaatccaat tcttccatcg aacgaaataa ttttgcccat cgtttatcaa caatccgtgc
    13441 atcactttcc agtctatccc aaacaataag ttttgcatta attaaaccaa cgccaatctc
    13501 tcctaaacgt ttcggtgaat cacctaaaat aaatgcttct actgcttgtg ttgctttctc
    13561 attggctaac cgtaactcat atcgattata aataccatat ttcgttaatg cttctaattc
    13621 tgaaattttt tctcggttcg caatttcaaa acgtttctca taaaaattaa aaaagagagg
    13681 actctttcgc gatccaaaat acaaagataa tccttcactt cgaaaatagc catcatctaa
    13741 aaaacaaccg ccaatattct ggaatctctt aaagtccatt tctaataaac ctgctttttg
    13801 tttttctagt aattcaaaga gatcgaaatg ttcttccgtt tccaatcccc aaagttcatc
    13861 taacgcaata tcgaatcggc tacattgcac acgtgtataa ccagcttcca actttccttg
    13921 atttttccaa atggaatata gccatgttcg ccaatctgtt tgttgttcac gcatgacttc
    13981 ctcatattcc cggcaacctt gtcctgaaaa ttgcacaagt acattttctg ttacagttcg
    14041 ttttgaatgc cagaacaccc agatattccc acgaacagaa agacagtcaa aaagatacaa
    14101 gcccgttgga cgtgtttcaa attctgtcag atccattcgt aatacacgtt gaatcactgt
    14161 tttgtagtct gtcccaaaaa aggaaagacg caaataatca atcattgctc gaagatctat
    14221 tccgcttttc atatacaatc gaaagcgttc cttgattcgt tgttcaaaat cctctgggat
    14281 ttttcttttc ccttgttcca tctttctaat gtaatctttt gagcagccca tcaattgcgc
    14341 aaatcttgct tgtgatagcg gctttttatc tccacttata ttatgaattg cacttaactt
    14401 ctttctgtag ctttttagct caatcggtgt cattttctcg ctccttttat aaaataacaa
    14461 aatcgatttt ttttaaccct ttagaaacac cgatatatca actagtcccc ctcgtttttt
    14521 gggaaaagta gtacaactgc tgaccttaga taatcacttg atacaaaagg gtttataaaa
    14581 cttttttcta tgtgttatgt tatttctgcc ccccatatta tatatcgggg ggctttacac
    14641 aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg cacacgctgc tagcgagtgc
    14701 gcattatagg cccatgcttc gcagccttaa tgcgcaccgc caggcagagt gcggctacca
    14761 gactacgtct agagccctcc cgatagataa taactacaaa attaaatgat ctaattttgt
    14821 tatttcacta tatttttcag gagaaaaaac gcgataaatt gacaaagtta aatcctcttc
    14881 taaatctact cgataatgat acaatcgagt caatcgttct atatcttttt tcgttagcaa
    14941 tacttgatta ataacttttc ctgtaaaaac acgttctttg cgcaaaaagc ggtaatcagt
    15001 tacttgttca atacgattca accaatcata aaacgttctg gaagaaagag aaggattaat
    15061 cattcgtatt gctgtagcta ctctataata acaactctct gccatttaac ttcacctcct
    15121 tagttgatta caaaatatgc gcatcagaaa aacttgttcc ttttggatct tttgctaaat
    15181 acatttggcg ctctagttgt tcttgtaaca actgattatc atctcccatc gtttgatctt
    15241 gactatcctc ttttactgta aaatcataga caatcatttc agtaaatttc tcaaattctt
    15301 tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa ctctacaggt acatcaaatc
    15361 ctggaaaagc atatccacga ccaggttgat ctttgttgta aaatggctta tttttaaaat
    15421 cttctccaaa agtcattaaa taaccaatgg gagacaatcg accaacagaa atacggaatg
    15481 ttaaattatc ccgaacacca cttggaaaat attctgcatc tggtcgttgc attgctaaaa
    15541 taatgtacat ccctgcttga cgacccttta atactaatgg ctgaatcaat ttatcgactc
    15601 tatttctcgc ttccatatcc aacgaaccat aaaaagctgc ccattcatca aaaaagataa
    15661 aatgcggtgg taacccataa taagcataat ttttccctgg ttcatattta ggatctttca
    15721 tggttttcat ataggcatac cgttgattca tttcttttaa tgcatttcgt aaacaaataa
    15781 tcatttcttc atttattttg taaaacacat gtcctttgaa tacatcgacg tcacctaatg
    15841 ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt cccaacactt aaacaagctt
    15901 gaattaaaga atacaataaa taggttttcc caccaccgat tgcaccagca atcaacatat
    15961 ttggtgattg atcatatacc caatccaaag ttttcattaa atgaatacgt ccattttctg
    16021 ctatacattg atcaatagga atccgaccag aatctgaaag gaatacataa ttgattcggg
    16081 ctttttctcg ttcttcacga attaaatcac gcaaaaatac ttcagataac aaatgaccca
    16141 tttttaaaaa tcgttcgtga aaacgcccac catctaaagg aaaagaaacc actaattctt
    16201 gtttcttttg ataaaaataa acttttggta atctcatttt ttcccttgtt tgtttctcac
    16261 taacttgttt ctttgtataa acaaaccgat tgtcaattaa cattcgaaca agtaactgac
    16321 gccaataaac agaggcaaaa tatccttctt ttagtcgcga ccaatgcatc aaccctaaaa
    16381 ttaacccact aaccagcaac aaatacaaca caatacttat tccaggaata agatacattt
    16441 gccaattcga aaacgtactt aaacgtggat aaaatacttt ccaactaaac caacctaaca
    16501 gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa ccataaataa cgattgaaat
    16561 agcgaattcg ccgaccacga tacttgaaaa aatgagcttt caattgtcac attcctcctt
    16621 tctgttttta cttttcctgt gctaaaaact tttgacaaaa agcaactaat acaggaatat
    16681 cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg atttttataa gggtgtgcac
    16741 acatattaca tattcgtact atacgaatcc aaatcacttc ctgatgatct tgtttccact
    16801 ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat acactgccca ataaccgcac
    16861 aaatatatgc atttgtttgt acaaaaatcg ttggattgtc tccaaaatac tcaatcaatt
    16921 gttggatatt ttgataacaa gcacaatctc tcaaagtatt tgattggttt tatctgaatt
    16981 caaaatctcc ccttccttct atcttagata aaaagaatag gaagactcct tcctattctt
    17041 tcaaaaaaac cttatttttt ctctttttcc ttagaatcaa cacttggttg tttttgatct
    17101 aaaccttttt cttgtgattg tttcatatta ggattatttg ttggagcttc tactttctct
    17161 aatcctttcg cataaatcac taaatctgaa tattcacgat tgccatcttg tgctggaata
    17221 tataccacat gatattctgg atcaattaat ttcacaggat cacgtcgttt aaaaccagtc
    17281 aaatctacat ctgctggaaa tttaactcgt cgtgcaatct ttggttcatc tggatttcca
    17341 aaaactaatt gtgctcgctg tgatcgaatt tctcccgtat aatcgcctgt ttcattatca
    17401 aatacacgat cagactcttc taatgcgcca aagcgtacgg ctcctaaaca agtttcatct
    17461 aatgcttctg taatgtcata attatttgga aattttaatc ccatcttaca aaacctcccc
    17521 aatttttaca ccagttaaaa agacaccaac atcttgatag ccagttgttc gacgctctgt
    17581 ttgtcccata tcatttgttt cacgactatt cactcgttct ttacgaaaaa caaaataagc
    17641 ttcttcaaat gaaatacgca cctctttttc tggtaatcgt aatcttttat cctcttcagc
    17701 tgtgagataa acaaaaaaag caggctgttc ttctcgcgaa cactctgctt gtaattgaac
    17761 atatcgccca gacacaccat taatttcctt tggtggatta ggttcccgtg aacccaataa
    17821 aaatacttga cctaaaaact ctttcttcat acgcaatgct ttttctgcca taaagttttt
    17881 cctcctctta aaaaccaaaa taaattctaa atatttctac attacaatca aacaagcggt
    17941 taaaaatcct aaccgcttgt tcctcatgac taatctttgg ctttacgtac tttataaagt
    18001 gttataccta aagcaatgac taatactact aatccaagaa ccataagtat actacttgtt
    18061 tgttcacctg tttttggcaa cattggtaca acagtagctg gtttaacccg ttgttctggt
    18121 ttcactggtt gcggtggttt tggcgcttcc acaatttgaa ccgtttgatc tttgtttgtt
    18181 agatctacat tgtgtttcgc cacttcttca aacgtttctt tctcatcttc cgttttcgct
    18241 tggagccatt ccgcaaaact caagcctttt cctgcaagat tgcgaccgtc cactaagaat
    18301 tcaaataaga attccatcat ttctttatcg gccgcaaaga ctttttccgc atgagtgcct
    18361 tcaaatggtg tgccgtctgg tgttaagtag ccttgaacaa ttaagcgata gatttgacca
    18421 atcgcaagac cgtctacttt gacccattca agcacagttt gatttggcaa ggccaagatc
    18481 tcttttccgc catttttatt cgtcgctttc gtcgtaatgg ttggattact gattttgatg
    18541 gtttggtcat tgtcggtaat atctgcgtga gaagccaata atacgccttc tctaaacaga
    18601 tcttcaaaga ccacgacttc tttgcctcgc aacgcacgtg cgtctagaat aaagtctaga
    18661 ttgactgtac catccgttgt ttctgccaca aacgttaagg tacttgtgac ttcttttcca
    18721 tctactagca ctggtttttc cgttactttg tccattagaa caccgttgac tgtgtatgtt
    18781 tttccaacga ccaaatcgtg gtatttcaca gcgtctgtaa tggtgacttt cgttaatgga
    18841 ttaacctctt tcaagccagt gggtttatgt gtagccgttg tacgtacact tgggttagtt
    18901 actcgtaccg tttgcccttt gtcgttgatg tctttatggt ttgctagttc ttcatcattt
    18961 ctaaaaagac tttcaaacac gacgatttca cgaccattta tacctgtcaa atcaaaggta
    19021 aatggtacga tcaccgtttc attttctgct ttggcaataa agccaacact cgtttctacc
    19081 gtatttccat gcacaagcaa gggttcaccg gttgctttat ccattaagat ccctttgacc
    19141 aagtatgctt ttcctggaag aatctcggta atttcaacgg tatcgtttaa ggtaaacaat
    19201 cctgttgggt ctgcgactgt caatccgtct tcaaagacag ccgtggtatg aacggttgga
    19261 ttattcactt tcacactttg ggcttcatcg ttgatatccg catgtgctgc aacttcaatg
    19321 ccatcacgga aaagtttttc aaaaacaacg atcgtttgac ctttcagaac actagcatta
    19381 accacaaatt caaggtcttt tgtgccattg actttgtctg caataaaggc caaggtactt
    19441 gtgatttctt tgccatcttg taaaattggc gcattcgttg ccttgttcat caatactcct
    19501 gaaatggtgt attcttttcc gacaaacaag ttggtataag aaacactgtc tttaatgacg
    19561 atcgtttcta atggatcaac cactttttca ccatcggctt tatttgacgc ttgtgtttct
    19621 agttttggtg gaatcaagta attacgtaca gaaaccacta acgtttcccc atcatctggc
    19681 gcattttgtt tgaatgcaac cgtgaatgct tgtttatctg tatttggttg gaaactttct
    19741 aatggggcat tttccaagaa gtaatagtca tcggcaacta gacctttcac taaaatttcc
    19801 ccgttttgat cagtggtata ttcaccaatt gcttgatttt taccgttata caaggtaaag
    19861 ttcacacctg cgagcggcgt tttcttcttg ttccattctt catacttgaa taattttaca
    19921 cttttccgtg cttgacggtt cgcaaaatgg atcactttga tcggttctgt atcattggta
    19981 atcgagaaca ctaatgggtt ttgattcaaa acgtaattta cgggggcttt gatttcttcc
    20041 aaacgatagc gatcttttcc ataagtaagt gtaccgttag ttaggaaaaa gcctttttca
    20101 ttggttttaa agatatccgt tgtttctgta tcatttggaa tctccattgt gacataacga
    20161 ttggctaaag tatcaaaaat cttgaattct gtattagcaa gtggaatcac ttttcctgtt
    20221 tcttgatcca ctttcaccac tttaagacgt tgttcaatta ctttatcttc taaaacataa
    20281 tggaaagttt gcccattttc agaaattgtg acttcaagtg gttcaaatag tttataccct
    20341 tcttttcctt tggtttcaga aagaatgtac gtatcaaaga caacggtatt tttaccaaaa
    20401 gtagcataac catcattgtc tgtcacaacg gttttcacca ctttgccagt tgtttttgac
    20461 gtcaaagtga tttcaatgcc agctaatttt ggtttgttat ctggattcgt tgaatcgact
    20521 aaatctttat tagcaacttt cactaaatca atggttcctt tgatcacttg atcttttgcg
    20581 gtcgcattga cagtttttcc aggttcaaca acaatttcaa ttgcttcatc agaaagcaca
    20641 taaccttttg gcgctttgat ttcttttaaa acatatttgc caggatcaag atctttaatt
    20701 tctgcctgta ctttgccgtt aatttttttc aaggtaattt ctttaatctt tttcccatct
    20761 gcttgataca aaccatagac cgcattttct aaagaagctg caccttgtgc tttcacacca
    20821 gtttccgcat cttctttaaa cacacgaatg tttccttgtt ttagaatttg taaattaata
    20881 acagacgcat taggttctgt tggatgtaac acaccaattg tttgagctcc tggacgacga
    20941 taggctaatg gcgcaccttg aactttaatt ttattcaatt gaatttttgc tttatccggt
    21001 gcctcttttg tgactgtaat ccatacttca ttgccgtgaa tagatacatt gacacctttt
    21061 ggtttttgaa tgtctttcac atatttcaaa aaatcatttt tattctttac tttcaacgtt
    21121 tcgccaattt ttaccgtatg tgtttgatta ttaaaagttg ctttttcacc aaagttatta
    21181 atcaaacgtt gaatttctgc ttttcgacga tcataatcaa cacggatatt tttgattggg
    21241 atttttgtgc ccaaagcttc ccaaatcata aattgtgccg caatgtattg ttcatcggat
    21301 ttgtctttat ttgtgacata tcctaaataa gagatcaagc ttgttttttt ccgtacattt
    21361 gaatttttca aatagttttt caaagcagta gctgtataaa cttgaccttc tgcggctgag
    21421 acaccaggct caatacaaaa cgccacttgt ccattaatct caaaacgaac catataattt
    21481 aaagtgaagt ttagattgtt cggactagaa gtaacaccaa tattttcgcc ccaatcaatt
    21541 ttccgaccac ctaaaacagc tgcattggtt gcacggcgaa cacgaaccga acgattggtt
    21601 ggtactgtta ctaacagtgt aggtgtacct gtttttggaa tgttctgctc ttttttcttt
    21661 tgattttcaa tactagccgt agattctttg tttggtttcg ttggttgatt ttgactagct
    21721 gcggttgaag agattgtttt ttcaacgttt tcctcatttt ttccttcttt gtcagcagta
    21781 gccgctggtt gttccactgt ttcttctatt cgtttctttt cttccgcttg tttctcagaa
    21841 gactctggct gcacagaagg cgttgcttcg gcagcttttt ctccttctag agtggacgtt
    21901 tctgaagcag gggttgtaat tgaggcagtt tcttcaactg gttgtgaagt accaggacta
    21961 ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag tcgttttgga attgaccgtt
    22021 ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac cagctgggac cccggcttct
    22081 gctggttgga cttctgtcac ttcagaagcg acttgggcaa tggcttccat cgtaggttgt
    22141 atagccgtcg tttcttctgc atgtgcttga gcagttccca ccaagaaaca gaattgagaa
    22201 aaagccacca atgcgagtgc tttcaaccaa ttcattttcg tttttgcata cacaagaatt
    22261 cctccttttc ctgagaattt tatataataa aacaaaacgc cgttaccaaa agtgataacg
    22321 acgtgaactg tctcttctat ttttatctgg cgctcttatc tgaacacatt tcatcaatga
    22381 ttttaccctc ccactataat tacagaacaa aaaaagaacc tgcttcgtgg aaatcaactg
    22441 tccatttccc aattggaata ccagcttttt tggcaacttc accatcacca aatctcatgc
    22501 cataccaatt actaccttca tccttaattt ctttccaacc atatgacata gcttcatcta
    22561 atgaatcaaa aatcatgaca ctattgccaa ttgttacagg atctggctta agatcttctg
    22621 gaagagaaat atcaatcata ccataatgat ccgattcctc agtattattg tcattactag
    22681 ctactggttt ggttgttcta ctggcttatt ttgatacaac aagtactctt attcttcttt
    22741 tacttctccg ttttcaattt cccattcaaa ccactccgca aagtcttgct taaaaccatc
    22801 tactcgatac ctattttgta aataggcatc ttctgacgtc gtaggatcta aataggtagg
    22861 tttattctct ggacgagcat agacacctac ccatgtttcg taatctcctt caaaataact
    22921 atctaccaaa tagccttttt ttctaagaat ctcccgaact tgatttttaa ttattgatac
    22981 ttgttcaaaa ccatcactca ttctatcacc tcaaataact ctagattctc ataaatattc
    23041 ccgacaattt caatcctatc aatgatctct aacaaatcgc tacttatgct tccccattcg
    23101 tataagaatt tcccttccgc aaaataaatt ttccataaac ttcttcatga tcgtcccaac
    23161 caatatcact ctcgaaaaat tccacgttgt tcttatcttt tagctcagta aatgccataa
    23221 gttcaacatg ttttccaacc tcgaaacaat cacaccaccc atcaaccgtt acacgtgtgc
    23281 cttcaatacc taagaaactt tgtcctcgtg tattaaacac taaaactttc cgcatttttt
    23341 gctcattaat taatcacgcg cgaaatctag gaatcacaac ttattcgctt cttctttata
    23401 aataaaaaaa ctccaataat cggaaattac tatcttttcc tcattccttt tttaaatctt
    23461 catacttctt cttatctttg tcctaaccta ataacacaaa ttttgaagaa aaactcagta
    23521 aaacctgatt ctttttttat cttcacaaca accaactttt tcttcagatc cttttactag
    23581 aacttgtata caaaaattgt tgaatgctga tataatttgc ggctacttga aaaaacgata
    23641 gatgtaaaaa gataaataga atcatttttt attctatatc agtctatgat tcattgctta
    23701 tcaatcaccc aacccattcg atgccgtttt ttttagagaa atttaaaaaa gaccgtacga
    23761 atctcgcatt atccgattac ttttccagaa aagatcccca attctgtata aataacccta
    23821 tcttcttgtt ggtttcattt tctcatataa gaattcagtc aatctaccca ctatgaccat
    23881 tactacgtcc aacacaactg acatattaac cacgtaagta ttccctcaca gatctccgaa
    23941 tctccatggt atggattccc atcctgacgc tgcagaggac aacatatttc atgtggctct
    24001 gtaccctttt gatttggaaa cactacaaat caatactcat ctataactta tcaagcgaac
    24061 tttcgctagg cttctagtga ttactaaatt gttggataac atacatagcc cattttatct
    24121 agctgttatt cataacagtt agttcttgta ttacttcttc cgcttgtgtt gccattgagc
    24181 ttgtcaaaaa gttacttaga aaaaagctgc cagtaaagca actttcaaca aacccttttt
    24241 gtttcttatc atatcaagaa acctcctttt ataaattttt tctataataa aaaacgtcga
    24301 taacttaata agtatcgacg taaactattt ttatatcccg taaatcaaaa attcta
//
LOCUS       DAYYGY010000042        24292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000042 DAYYGY010000000
VERSION     DAYYGY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.043"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..606
                     /locus_tag="ACHA52_001595"
     CDS             289..606
                     /locus_tag="ACHA52_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8940296.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="ACHA52_001596"
     CDS             complement(697..1062)
                     /locus_tag="ACHA52_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940297.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="ACHA52_001597"
     CDS             1270..1752
                     /locus_tag="ACHA52_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940298.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="ACHA52_001598"
     CDS             1949..2839
                     /locus_tag="ACHA52_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8940299.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="ACHA52_001599"
     CDS             3165..5639
                     /locus_tag="ACHA52_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8940300.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="ACHA52_001600"
     CDS             complement(5742..7478)
                     /locus_tag="ACHA52_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8940301.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="ACHA52_001601"
     CDS             complement(7475..8179)
                     /locus_tag="ACHA52_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8940302.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="ACHA52_001602"
     CDS             8310..8813
                     /locus_tag="ACHA52_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8940303.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="ACHA52_001603"
     CDS             8773..9111
                     /locus_tag="ACHA52_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8940304.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="ACHA52_001604"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="ACHA52_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8940305.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="ACHA52_001605"
     CDS             10662..11240
                     /locus_tag="ACHA52_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8940306.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="ACHA52_001606"
     CDS             11244..11765
                     /locus_tag="ACHA52_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8940307.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="ACHA52_001607"
     CDS             complement(11844..12398)
                     /locus_tag="ACHA52_001607"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8940308.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="ACHA52_001608"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="ACHA52_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8940309.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="ACHA52_001609"
     CDS             12938..13189
                     /locus_tag="ACHA52_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8940310.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="ACHA52_001610"
     CDS             13313..14104
                     /locus_tag="ACHA52_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940311.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="ACHA52_001611"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="ACHA52_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8940312.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="ACHA52_001612"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="ACHA52_001612"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8940313.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="ACHA52_001613"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="ACHA52_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8940314.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="ACHA52_001614"
     CDS             16339..16581
                     /locus_tag="ACHA52_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8940315.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16669..16815)
                     /locus_tag="ACHA52_001615"
                     /pseudo
     CDS             complement(16669..16815)
                     /locus_tag="ACHA52_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16872..17186
                     /locus_tag="ACHA52_001616"
                     /pseudo
     CDS             16872..17186
                     /locus_tag="ACHA52_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            17215..18474
                     /locus_tag="ACHA52_001617"
     CDS             17215..18474
                     /locus_tag="ACHA52_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8940316.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="ACHA52_001618"
     CDS             18527..19423
                     /locus_tag="ACHA52_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940317.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="ACHA52_001619"
     CDS             19434..20303
                     /locus_tag="ACHA52_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940318.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="ACHA52_001620"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="ACHA52_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="ACHA52_001621"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="ACHA52_001621"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8940319.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="ACHA52_001622"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="ACHA52_001622"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8940320.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..24208
                     /locus_tag="ACHA52_001623"
     CDS             23711..24208
                     /locus_tag="ACHA52_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8940321.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         8044 a         4148 c         5015 g         7085 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
    24181 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
    24241 caagaataaa aagagccgga aagacagtgg gcatttctgt ttttcggttt tt
//
LOCUS       DAYYGY010000043        23821 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000043 DAYYGY010000000
VERSION     DAYYGY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.044"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..779)
                     /locus_tag="ACHA52_001624"
     CDS             complement(45..779)
                     /locus_tag="ACHA52_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8940322.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="ACHA52_001625"
     CDS             1106..1516
                     /locus_tag="ACHA52_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8940323.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="ACHA52_001626"
     CDS             1509..2210
                     /locus_tag="ACHA52_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8940324.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="ACHA52_001627"
     CDS             2210..3796
                     /locus_tag="ACHA52_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940325.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="ACHA52_001628"
     CDS             3815..4036
                     /locus_tag="ACHA52_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8940326.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="ACHA52_001629"
     CDS             4033..4794
                     /locus_tag="ACHA52_001629"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8940327.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="ACHA52_001630"
     CDS             5174..5683
                     /locus_tag="ACHA52_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8940328.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="ACHA52_001631"
     CDS             complement(5758..6297)
                     /locus_tag="ACHA52_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8940329.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="ACHA52_001632"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="ACHA52_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8940330.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7310..8161
                     /locus_tag="ACHA52_001633"
     CDS             7310..8161
                     /locus_tag="ACHA52_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8940331.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8189..8824
                     /locus_tag="ACHA52_001634"
     CDS             8189..8824
                     /locus_tag="ACHA52_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8940332.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="ACHA52_001635"
     CDS             8844..9617
                     /locus_tag="ACHA52_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8940333.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="ACHA52_001636"
     CDS             10025..10822
                     /locus_tag="ACHA52_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8940334.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="ACHA52_001637"
     CDS             10800..11213
                     /locus_tag="ACHA52_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8940335.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="ACHA52_001638"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="ACHA52_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8940336.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="ACHA52_001639"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="ACHA52_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8940337.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="ACHA52_001640"
     CDS             15990..16550
                     /locus_tag="ACHA52_001640"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8940338.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="ACHA52_001641"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="ACHA52_001641"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8940339.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="ACHA52_001642"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="ACHA52_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8940340.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="ACHA52_001643"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="ACHA52_001643"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8940341.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="ACHA52_001644"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="ACHA52_001644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8940342.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="ACHA52_001645"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="ACHA52_001645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8940343.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="ACHA52_001646"
     CDS             21897..22475
                     /locus_tag="ACHA52_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8940344.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="ACHA52_001647"
     CDS             22527..23009
                     /locus_tag="ACHA52_001647"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8940345.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="ACHA52_001648"
     CDS             23126..23500
                     /locus_tag="ACHA52_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940346.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23613..>23821)
                     /locus_tag="ACHA52_001649"
     CDS             complement(23613..>23821)
                     /locus_tag="ACHA52_001649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940347.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7773 a         4285 c         5115 g         6648 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga cagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatggggt attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagttt
    23821 t
//
LOCUS       DAYYGY010000044        23775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000044 DAYYGY010000000
VERSION     DAYYGY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.045"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..1322)
                     /locus_tag="ACHA52_001650"
     CDS             complement(60..1322)
                     /locus_tag="ACHA52_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940348.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1315..2214)
                     /locus_tag="ACHA52_001651"
     CDS             complement(1315..2214)
                     /locus_tag="ACHA52_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940349.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2359..4137)
                     /gene="uvrC"
                     /locus_tag="ACHA52_001652"
     CDS             complement(2359..4137)
                     /gene="uvrC"
                     /locus_tag="ACHA52_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8940350.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4319..4633)
                     /gene="trxA"
                     /locus_tag="ACHA52_001653"
     CDS             complement(4319..4633)
                     /gene="trxA"
                     /locus_tag="ACHA52_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8940351.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4720..7080)
                     /locus_tag="ACHA52_001654"
     CDS             complement(4720..7080)
                     /locus_tag="ACHA52_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8940352.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7195..7743)
                     /locus_tag="ACHA52_001655"
     CDS             complement(7195..7743)
                     /locus_tag="ACHA52_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8940353.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7801..8238)
                     /gene="zapA"
                     /locus_tag="ACHA52_001656"
     CDS             complement(7801..8238)
                     /gene="zapA"
                     /locus_tag="ACHA52_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8940354.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8440..9357
                     /gene="rnhC"
                     /locus_tag="ACHA52_001657"
     CDS             8440..9357
                     /gene="rnhC"
                     /locus_tag="ACHA52_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8940355.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9613..10524)
                     /locus_tag="ACHA52_001658"
     CDS             complement(9613..10524)
                     /locus_tag="ACHA52_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8940356.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10927..11505)
                     /locus_tag="ACHA52_001659"
     CDS             complement(10927..11505)
                     /locus_tag="ACHA52_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8940357.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11525..12193)
                     /locus_tag="ACHA52_001660"
     CDS             complement(11525..12193)
                     /locus_tag="ACHA52_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940358.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12208..13278)
                     /locus_tag="ACHA52_001661"
     CDS             complement(12208..13278)
                     /locus_tag="ACHA52_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940359.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13449..14813)
                     /locus_tag="ACHA52_001662"
     CDS             complement(13449..14813)
                     /locus_tag="ACHA52_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8940360.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14810..16369)
                     /locus_tag="ACHA52_001663"
     CDS             complement(14810..16369)
                     /locus_tag="ACHA52_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940361.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16425..18905)
                     /locus_tag="ACHA52_001664"
     CDS             complement(16425..18905)
                     /locus_tag="ACHA52_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940362.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18889..19224)
                     /locus_tag="ACHA52_001665"
     CDS             complement(18889..19224)
                     /locus_tag="ACHA52_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940363.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19238..20272)
                     /locus_tag="ACHA52_001666"
     CDS             complement(19238..20272)
                     /locus_tag="ACHA52_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8940364.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20362..21042)
                     /locus_tag="ACHA52_001667"
     CDS             complement(20362..21042)
                     /locus_tag="ACHA52_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8940365.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21326..22429)
                     /locus_tag="ACHA52_001668"
     CDS             complement(21326..22429)
                     /locus_tag="ACHA52_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8940366.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(23058..23528)
                     /locus_tag="ACHA52_001669"
     CDS             complement(23058..23528)
                     /locus_tag="ACHA52_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8940367.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
BASE COUNT         6775 a         5002 c         4016 g         7982 t
ORIGIN      
        1 atacaaaaaa gaagctggga acaataaatg tccagcttcc ttctatattt ttctatattt
       61 tatgcttttt ttcgttcatt tctcataatt gaaatcgatt gtttcaatga actccataat
      121 ccgccttcgc tatacacgag tacattcgat ttgtacatgt ttgcagacaa gatcatcaat
      181 aagaatgtta cggctacaag taccaacaag gaaatcaatg ctccacccat accgactgtc
      241 tcatttgcta agcgaaccgg cataatatat gaagataaga atggaatata cgaagtgatc
      301 cgaataacga tattgtttgg atctgaagct cctagtatca atccaagcat atatccgcct
      361 agagatagat aagtcactgg caaaattgct tttgctgtat cttctgcttt gttgaccaaa
      421 gagccacata aagctgctaa aacagaaaaa atcaaaatgc caaagatcat gaatagaaga
      481 gaataccaca agaatggtcc aatcaagtta tccaatgaga taccgtctaa tatatttttg
      541 accatttcca tatctttgaa ttgactgtat cctataccaa aaataatacc gtataatgcc
      601 atttgcgtta atgcaacaag taaaacacct gttagttttc cgtaaaaatg cttttgtgca
      661 gtcgtactgg acaggatcac ttccatgatc cgcgtgcctt tttcagaagc gatctcttgt
      721 gcaatgatct gcgcatacgt caaaataata atgaacaaga caatcgttgc tacataactt
      781 acgacatatt ggactgtcga gttatcttct ccaactgtca tttttccttt tgaatcaaaa
      841 ttgacttttt gtcgagtcaa acttgcttgt tggctcaagc tagctacttc ttctggtgaa
      901 atgcctaatt gacttgctcg aagcatcgat tgcaagccag tcagttgttg ttgtatcagt
      961 agttgtgtac tttgtcctaa tgaattttcg ctataaagca tcccatttat cttatcattc
     1021 tcagttgaga ctactaaata cgcatcgatt ttttcttccg aaagctcttt ttgtgctttt
     1081 tcttcagaat caactgcttt gaactcaaaa tcatctgttt tgacttggct catttgctga
     1141 ctgatttgct cattatcagc caatacgccg attttattga catcactatt ttgttgagca
     1201 aagtttcctg ctacataaat gatacccagc atgataaaag ggactaatat catgatcaag
     1261 aaagaaatcg acttaacatt ttttaaatag acatctttgg taataatcca aaacttacgc
     1321 atcgatttca cctgctttca atttgaaaat ctcttccaaa gttggcggtt gctggttaaa
     1381 cataggaatg tagccgaact gagtcgcacg agtaaagatt tctttcccag cttcaggatc
     1441 ttctaaggta atctctaacg tattgtcttc atgaaggatc acttttttca cgccttcgat
     1501 ttcttctact tcctgttggc ttaaaccaga ttccaagaat aacttcgtac ggccaaatga
     1561 ttcacgaatc tcatgtactt ttccattcaa aaccattttt ccgtttcgca acatgatcaa
     1621 atgatcgcag attttttcta cattatccat attgtggcta gaaaaaatga cacatgaacc
     1681 atgttttttc aattccaata ttccatcttt tagcaattca gcatttaccg gatctaaccc
     1741 gctgaatggc tcatccaaaa tgatcaattt aggttcatgg attaacgtgg cgatcagctg
     1801 gactttttgc tgatttcctt tagacaaaga ttttacttta tccgtctttt tcccttttac
     1861 ctgaaatttt tccatccaaa aatcgatttt tggttcaatc tcttttcttg atttccctct
     1921 aagttctgca aaataaagca gttggtcttg aatcgttact ttcggataaa gtccccgttc
     1981 ctccggaaga taaccgatga catcataatc tttctcccca agatgatggc cattccataa
     2041 cacttctcct tggtcttgtg tcaaaaaatc caagatcaaa cgaaaagtcg ttgtcttccc
     2101 agccccgttt tggccaatta gccccatgat ttttccatcg gggatttgga aagagacatg
     2161 atcgacagct gtgtagttcc caaaagtttt aacaagatcc ttgacttcta acatttcttc
     2221 actccttctt caagaaatca catatatcct tagacaccag acgtaagcga tttattattt
     2281 accaacaaaa cgtaaacgct ctttttgttg tacataaaat gataggacac tttactttct
     2341 ttgtaaagtg ccctttcctc attcctgatg taatttatca aatacgtttt gtgcgacatt
     2401 tttcggcagt ccgatttctt gtaattcttc tgtcgtggct gctgtgatat ttttcaacga
     2461 tttgaactct ttaagcagca ttttttttcg ttttggtcct aacccttcaa tttcatctaa
     2521 tcgggaagca aaactgtttt tactgcgtag ctgccgatgg aaagtgattg cgaatcgatg
     2581 gacttcatct tggattcgct ggagtaagaa aaactctggt gaatttcgct caagcggtac
     2641 tacagccaaa tctataccga atagcaactc atttgtttta tgtttatcgt tttttgccat
     2701 gccagcaata ggaatatcga ttcctaattg attttctaag acatcttttg ccacatctac
     2761 ttgtcctttt cctccatcga tcaagatcaa atcaggtaaa ggcaaccctt cgcgaatcac
     2821 tcgtgaatat ctgcgataga tgacttctct cattgatgca taatcatccg gtccgttgac
     2881 tgtttttatt ttatatttcc gatattcttt tcttgccggt tttccatcaa tgaatacgac
     2941 catcgcagcc acaggattcg tccccatgat attagagtta tcaaaagact cgatacgtat
     3001 tggggtcggg atattcatcg cgtttcctaa acgttcaact gctccgatcg tccgttcctg
     3061 ttttctggca atcagatcaa accgttcatt caaagcaact gaggcattct tattcgccaa
     3121 ttcaactaat tttttctttt ctcctcgttt tggttgcagc actttcgtag ctaacaaagc
     3181 ttctacactg ggcttgtcga tcgtatcagg aatcaagact tctttaggaa tgaaatgctc
     3241 attttcctga taaaactgac cgaggtaagt caagaaatct tcttctgctt cgttatagaa
     3301 aggaaacatt gaaacatctc gttcaattag ctttccttgt cttacgaaaa acacttggac
     3361 acacatccat cctttgtcaa tcgcataacc aaatacatct cgatccagta aatcagtatt
     3421 tgtcattttt tggcgagtca tgattgtctc aatggcacgg atctggtctc ggtattctgc
     3481 agctttttca aactccatgt tttcagcagc tttttccatt ttctgatgga tctctgactc
     3541 gattttttcg tgtcctccgt ttaaaaactt tttcacttca tcaacgattt tcgtatagac
     3601 tttcggatct acgtcgaaat aataaggaca cagacattgt cccaaatgat agtaaagaca
     3661 aggctccttc gtctgactag gtttgcattt tctcagagga aataatcgat cgaggatctt
     3721 ctttgtctca ttcgccgcac cgacatcggg atatggtcca aaatacagtg ctttgtcttt
     3781 tagtacttta cgagtaatca ccaaacgggg atatttttca ttcgttatct taaggaaagg
     3841 ataggtttta tcatccttta acataatgtt gtatttagga tcatttttct ttatcagatt
     3901 gatctctaag agcaatgctt cgatattcga ttctgtaacg atgtattcaa agtcttcgat
     3961 ttcactaaca agccgttccg ttttcgtatt atggctgcca gtaaaatatg agcggacacg
     4021 attttttaat attttggctt tgccgacata aatgatcgtt ccatttttat ctttcatcaa
     4081 gtaacagccg ggctgatcag gaagtaaagc taatttgttt ttgattcgtt cgttcatctg
     4141 gtactcccct tcatattcaa ttaaagttat tatagcatgt ctgtcaaaaa aacaatccag
     4201 cctgacaacg taattatact caaaaaatat taatttgaat aaccttaact caaccgcaaa
     4261 aaaaccgagg acggaactgg attcagtccc accctcagct tatttttgct gtaagttttt
     4321 ataaatattg atcgatcatt tgacgtagtt gttcttttga atgaacacca acagcttttt
     4381 ctactacttg accatctttt ttcaacatta atgttggaat gctcatgata ccgaattgtt
     4441 gaggagtttc tggattttca tccacatcca ttttagcaat tctgaattct tcttcatcat
     4501 attcttgttc caactgatcc aaaatcggtg cttgcatacg acatggacca caccaagttg
     4561 cccaaaaatc aatcaataca agtcctttat ctgtttcttc attaaatgtc ttatctgtaa
     4621 ttacttgtga catatcaaag acctccattt ttaaaatcta tgattagtat agcatttcta
     4681 tacttggata gcgaaccatc tgcttacctt tagtaagctt tacttaaatt taacaattgt
     4741 cgcaccattt ccgccttggt ttgccggtgc aaactcaaag cttttgacgc tccgatggtt
     4801 tttaagataa tcagtaattc ccttgcgaag tgcgcctgtt ccttttccgt ggacaatagt
     4861 cacttgagga taaccagcta gaatggcaga atccaaatat tgatctacct cacttagcgc
     4921 ttcttcgtat cgttttcctc gaaggtccaa ttgattcggc acgtgactac tagattcaga
     4981 tcgtacagca tggataactc gttgtgtcgg ctctttttga ggagcaacag gcgtcagctc
     5041 gtcttcggac acattcattt ttaatatacc gatttctact tgccactgat tcccgttttt
     5101 acgtaaaagc gtccctcttt ggccgtaagt cgtaaccaac acttcgtctc ccggtttcaa
     5161 cgttttttgt tctttcgcct tcttcaatac tttatttttc ttcaaagttt cttcttgatg
     5221 tagatttgct aattgggtct ttgcatcgat caactgatgc tctttcacat ttccttgccc
     5281 aatctgctgt tgcattttac gaatatcagc aatgattttt tctgcttttt cttccgcctc
     5341 tgacacgact tcgtttgctt ttttctttgc tttttccatt tctttttcac gttcttcaaa
     5401 gaaatagctg taagcttctt tcaaatcatc gtgaagttct tgtgcctcag aaacaaaatg
     5461 acgcatttcc agatattcgg tctctgccat tttccggcga ttctctaaat ctgtgatcat
     5521 ttcgtttaaa tcttggctct catcattcat caactgcttc gcttcatcaa taacttctgt
     5581 atcaagacct aatcggctag agatttcaaa cgcattgctt cgtccaggaa cgccaatcag
     5641 caaacgatac gttggactta gtgtatccac atcgaactcc atgctggcat taatcgtatt
     5701 tgcccgattg tatccataaa ctttcaattc aggataatgc gttgttgcca tcacataagc
     5761 agacttttta ccaagatcat ctaagattgc gattgctaga gcagcacctt cttgcggatc
     5821 tgttcctgca ccgagctcgt caaataatac cagacttttt tcattgactt ttgataaaat
     5881 atctacagtg ttcgtcatat gagaagaaaa ggtcgataga ctttgctcaa tcgactgttc
     5941 gtctcctata tccgcaaata cttcctcaaa aataccgatt tgactttctt cacctgcagg
     6001 aatcggtaat cctgcttgcc ccatcagttg taacagacct aaagtcttca acgtgatcgt
     6061 tttcccacct gtgttaggtc ctgtaatgac gatggcttga tagtcttttc cgattgtgat
     6121 gtcattagaa actacttttt cctgatcgat cagtggatgt cttgcttgtt tcaagacgac
     6181 atgattgtct tcactgattc ccggcacgat ggcttttact tctttaccaa atcgtgcttt
     6241 tgcattcatc agatccattt taccaatcac ataagcattg tggatgattt cttgacgatg
     6301 gggaacaagt tcagcagaaa gttcacttaa aatacgttga atctcatttc gttcagcgat
     6361 ttggtattga cgcaaacgat tattcaaatc caccacttgt tttggttcaa taaataaagt
     6421 ctgtcctgaa gcactttgat cgtggactac accgccaaat acaccgcggt attcttgttt
     6481 gactgggata acgtagcggt catttcgcat ggtgatgatc gcatcactta aatactttgc
     6541 atttttccca cggacgatcc catctaactg ttcacgaaca gcttgttcac ttcgccgaat
     6601 gttctgtctt atgctcttta actcaggaga agcgtcatca gtcacgcgtc cgtcttcatc
     6661 gattgcttcc tttaaacggc ggctcaaaac agggatcgtg actaactgat ctgcccacat
     6721 atacaaacgt gcaaattcga tttcactgtc agaaagatca tcaatgaaac gattcaattc
     6781 gcttgtcgta gacaatactc tggaaacttg tgcaagctcc actccattca agtctgctcc
     6841 gatctcgatc cgtttcatat ggggacggat attttcaagt ttaggtatag ggatcccgcc
     6901 tcgcagacgt tgcactttca atccatcctc agtttcttga agccagtttg tgatcgtttg
     6961 tttctcactc actggtacca attctgccag ttcttctttt ccttgtgcag tcactacaaa
     7021 ttgtctgacc atctgtttga ctttttcaaa ttccaatgtt tcaaggattc ggttattcat
     7081 tttatcacct tatctttcac tatgaaaaaa gagctgtgcc ggacatacgc tacgcagcct
     7141 tcttaaataa actactctgt acaaaaaacg aaatattttc cagcactatt ttccttaatt
     7201 caaaatattc gttacccaaa gctcatacat cttgttggta agaaatggcg ttctctcaac
     7261 gataaaacga gcgaaactat gctcgccgaa ttgattttgg accacatcta atggaacaaa
     7321 ggcaagtaac tgcaaaatca aaaatatagt aacgtaagcc atgattagtg acaggatgcc
     7381 tcctgcaatc cagtctacct gtctcaagat cggcacatac gtcaatccat ggacaaaaac
     7441 ccccacaaat cgggtaagca gccagccaat tagcaaaata atcagaaaag cagttcctgc
     7501 ataaaaagct tcatctaggt gaaaagcaaa tacctgatca aaaaatgcta actttgatgt
     7561 tggggtgaca gctgggtatg gaatgtataa ttccaaatga cttgctaatg gtttgtaaaa
     7621 atgctgggca gcaataaatg acaatacata tccaatcgcg aatatgattt gcaaagcaaa
     7681 cccgcgacgt gctccagaaa aaaacgcaat tagcaataaa aataaaatca gcaaactcag
     7741 catgctacgt tcctttctct tctaactttt ttaaattttt cttattttga taaaaggatc
     7801 ttaattagaa gatttttgtt taatttgtcc ttttcgtttc tcgtttaaaa tctgttgtgc
     7861 ttccacatga tttttaatgg tatgatctgc ttgaccattt tctttcaata cttcccgagc
     7921 ttcgttctcg attgcttcaa tgcgtttcac acgattttca agctctgtaa atttgatcat
     7981 ttttttcttt aattctgctg tttcttcttc cagctccaag attctttctt gtttcttcaa
     8041 ttgatccgaa agcgcattga cagccatcaa gattgctgct tgttcattat ccatttgtgg
     8101 tgataattgt ttcaattctg ctaactgttc attgatcagc ttcgttacga tatccatatg
     8161 atgcttggtt tcccgtccga taatagtata tgtttggttg gcaatcactg ctttgtagcg
     8221 tgttttttcg tgcgccatac gaatcatttc ctcctatttg atgaaagttt tatttagtgt
     8281 accatgaagt tggttctttc accgttctcc acaaatattt ttcacatact taactattat
     8341 atgataaact ttaaaaaaat gctatgtctc gcaatctctt agaaaacgaa aagtcgtgag
     8401 ttgcggtata gttttatgta taaaaacgag gagaatttta tgagcaacgt cattatcaaa
     8461 gcaacaaacg accaacttag tcaaatgaaa gcatactatc gctcttattt gttaaataaa
     8521 cagattcctt atactagctt tgctgcaaaa aaagacggga cgaccattac cgcttataca
     8581 tccggaaaag tgatgtttca aggtacgaat gcagcaatag aagctgataa atggggggag
     8641 gcacaggata aacaagccaa gccagcaaaa acagcctctt ctttgccaaa aaatatcagt
     8701 caattatccg ttttaggtag tgatgaggtc ggaaatggca gctattttgg tccgctaaca
     8761 gtatgtgctg cttatgtgaa acgtgatcag cttgcagaat tacgtaaact tggtgttaga
     8821 gattcaaaag agttaaaaga tagccaaatc atccagcttg ctgacatctt aaaaaaaacc
     8881 atcccctata aactgcttgt cttatctcca gaaaaataca atgagatcca accaaaatac
     8941 aatgccgttc ggatgaaagt cgctttgcac aatcaagcga tccatttgct gctaaaaaaa
     9001 atcgagccag aaaaaccaga agcgattttg atcgatcagt ttacgccgga agcaaatttt
     9061 aaaaaatatg cgcgtttaga aaaaaatcaa ttacaagaaa aactgtactt cgttactaaa
     9121 ggagaacaat atcatttagc tgttgctgct gcttctatca tcagtcgtgc cagctttttg
     9181 gaagagttac acaaagcatc agctgaagct ggaatcaccc ttccatcagg agctggaacg
     9241 aaatctgacc aagtagcggc aaaactattg gagaaaggcg gcatgcctat gcttgaaaaa
     9301 tatgccaaac ttcattttgc caatacagaa aaagctttga agctgctaaa aaaataatgg
     9361 aactgtgaca aaagtcatgt cacagtccct tgttccgaat aaacggtgtt ctcatgccaa
     9421 ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt gaaattgctc
     9481 cttgtgacgc ggagttaaac ggcaggctca caacctcttc cttaaacggt gttcaaaagc
     9541 ttgcatctgc gataataagc ccggccaaac gaaaaatatg aggagctatt tttgtttggc
     9601 tgggcttatt tattactgaa aataagtggt caatgcttgt gtcaaacttg cagcaatctg
     9661 ctgctgataa ctgtctgtta ctaattcctt taaatcacta tcattattca tataacccat
     9721 ctcgattaaa atagaaggtt gaagattttc tcgaagaact tgatagttcc cagtggagat
     9781 tcctcttgaa tccagaggca gatcagtgaa ggagtcagca attgtctgtg ctaatgtttc
     9841 ctcttgtcca tagtaataat atgtcgtgat tccagttgct tcattcgcat attccgtaga
     9901 gtctgcgtgt aaactgataa agacatctgc ctttttttct tcactgtagt cgcatatatc
     9961 tccaagctgt actaattcat ctgtagtatg ggtcaaataa accgttgctc cagcatcctc
    10021 tagcgccttt tttactttta tggctgtact taatgttata tctttttctt ctgtttgttc
    10081 atcattgctt aatgccccag tatcatttcc gccatgccca ggatctaata caatcactgc
    10141 atcagatagc tcttccggtt ttgtttgtat attctttgtg actttggttc catcttcgag
    10201 ggctaaatat ccagtatagc catcatttgt cttgatctga taagcatttt ttgtttcaga
    10261 aagaaactcc gccagctctc cgccgtttat agttgcaagt ggggtagtta gcgagtcatc
    10321 tgtatatagt gtacggtcat tttttccgat tttgattaat gtctcttgtt ctgtatcatc
    10381 aaatgattcc tttgtctttg taaccgtact gttcgtactg ctgaccgttt gtccaactat
    10441 ctttttttga ccaaaaacat ctatttgaaa acagataata atgataatga ttgcaaatac
    10501 aaaagctcca attatttttt tcattcttga ctctcacctc gtagatccag actaagggtc
    10561 aaaacttaaa atcagcttaa gtacgcaaaa aaggggctgt aaaagatata accgtagaaa
    10621 ataacattac tgctaaaatg atttttttca tttttggcat atcctgaaat ctccttttga
    10681 ctattcgtgg tgattcatct acccaataaa tagtttcctt cattctacaa acaattaatt
    10741 tcttcgttat taaattaaaa cacttcctgg aacaccgaca taataaataa aaatataccc
    10801 tgataatcat gagagaataa caaatttgta tttatgaaaa actacctata taaaaaaaac
    10861 aagatataac gatcttcgtc atatcttgct tcttcaccta actatttttc tccctatata
    10921 acagctttac gcttctccta atacaccatt caaatataaa tcgatcaatt gcgccacatt
    10981 tagcggttct tccacaggtc ttttcgaccg agtgatgtaa ggtgctaaca ctaagaaaaa
    11041 atgccttgct gcgatttctt ttgaaatccc atttcttagc acagattggt ttgctgaaaa
    11101 gatttcttca aaaggttcac gataattttt cttccataga aagaaaagat gatctcttgt
    11161 ttcctttgaa agattgcttt caagatcgtg aagcatcaga aacaaatcga ccagatgtgt
    11221 ctcaatcaaa aactgcgcca tttttgtcaa ggttacttga aatcccgttt cgcttgtttg
    11281 aacgatttct ctcaaagctt ttccaacatc ttttaagtgg ttttctaaaa ctgccgtata
    11341 tagtttttct ttatcaccga aataatgata gagattgggt tgggtgatcc caactttttg
    11401 tgcgatctct cttgttgaag tgccgagata tcccttggac ataaacaagt cagtcgcttc
    11461 tttcaaaatt ttttgctgtg tatctttgtt gtatcgatgg accatttttc cacctgcttt
    11521 ttggttattc tttttctttc aatacaccat cttccatcat aaatattcta tcgcatttgt
    11581 caatcagccg tgtatcgtga gtaaccatga tcgttgcttt atttttgtct tttgattctt
    11641 ttgccaagat atcgactact tctcgtgcct tttctgaatc caaactagca gttggttcat
    11701 ctgctagaat aatggacgga tcattgtaca atgcacgagc gattgcgacc ctttgccgtt
    11761 ctccaccaga tagttcttct ggatacttgt tgatcaactt atccacgcca agctgctcaa
    11821 ataattcttt tgcttctttt tgacgatgtt catgtttgat cttatctact aataataact
    11881 gcttttttac tgtcagaaaa ggaaccagat tggaagcttg caaaataaag ccaatttctt
    11941 taaaacgtag tttaacgcgt tcactttctt tttgattact gaattcttga tcattgataa
    12001 atacagagcc actgctaggc gtctgcaatc cgcctgccaa agtcaaaaaa gtacttttac
    12061 ctgaaccaga aggaccgatg attgcaacaa attccccttt atcgatactc atggttgttt
    12121 ctttcaatgc tttaactact tggtcgccat cttggaaact tttttctact tctttaaatt
    12181 ctattgcttt catgctcttc ctccttctta tccaattgcc ttcaacggat cgatttttac
    12241 gattgtccga acagaaaata gtgcgcctaa cacagctact accagcatca acaagctaat
    12301 gacgccaaaa aacagccaat tgctttggaa tggaaccgca tcaggtagaa caagggatgt
    12361 tcccactgtt cctaatagac caagaagaat accaacaaat gataaaatga atgtttgtgc
    12421 cactacagaa cgcgcaataa atcctcctgt gatcccttgt gctttcatta caccaaagat
    12481 attaatcttt tgcatcgtca gtacataaat gaaaatcccg atcacaatag ccgcaataac
    12541 gatcaaaaat ccgatcataa atccaaaagt caatacttgc gcattatatc caggtaattc
    12601 attgatgaat ttagaaattt tgatctgttc taaatcttca ggtaaattac tgattttacc
    12661 tctgacaact attgcgttaa tccgtgcatt ttcacttgta tcttcttttt cgaagcggat
    12721 ctcttggtaa gcattgatgg ttgtatagag aactggtgag acattgaatt tggcatgatc
    12781 cgtaaagcca gttagtttaa acgtcttgtc actaccagat aacttaactg tatctcctac
    12841 ggctagacca tattcttctt tcaaactaat atcgcctact gcctcatcat cgttatcaaa
    12901 agcttttcct tcaacaagat tgggcatgat gaattgattt ttatcgatac cgaagaagct
    12961 aacattgatt tttccttctt ctgtactgtc tttactagtc acgactccag gtgtctgggc
    13021 aagatacgcc actttcccac cttcgacttc ttctgctatc ttcttagtaa tcatggacat
    13081 ccctagattt gaatttgcat ctttcgaaag cacaatcgaa tctgcctccc acttgtcgac
    13141 tgctgttcga ttatcttgag ctaagccata agctaaccca gtcaaaaaaa ataccaggta
    13201 ggcgatcaaa aacatcaccc ctgcaactaa cgcataacgt aactttgaat gcataatttc
    13261 atttaatgct aaaaacattt aactcctcct cctgaaataa tcattttata caacaatttt
    13321 atcacttgat aaatttttgg cgaatattat gctcaaaaaa ttcttccgct atttcattat
    13381 atgaattttt tattctcgat tgattaatat ttattatatc cccacacaat aaaagaccgt
    13441 gatatttctc acagtctttt atctactcta ttgctatttg ctctcttagc tagtacttta
    13501 aaagctaact cattgaggta gtgaaaacga gaggtgtgtt cagacagaaa tccaaaatcc
    13561 tcaactgcta gctgtggaat cgtagttttg tgtttcacga tatccgcaag caggttcaaa
    13621 tctgataata agaattcttg gcttatcagc tgtccgccaa ttatttctaa tgtatccgga
    13681 tggtagatca gctttgcatt tactgttttg ttttcttcat cgtattgtgg aaatagatca
    13741 aattctcctg aacacgaacc ggtattttgt tccaagaatg gcgcttcatc ttctgtcagc
    13801 cctacagttc ctaaaaagta gtctataatg actgctgctg ttatcttttg tctgaggtcg
    13861 taagcggttg gctgaccact caaagttagc gctactgctc ttgccatatg tacagctcga
    13921 gtaactaaag gtaaaaagag atgccgttta tttttgtcaa aactgactgg gacgatatct
    13981 ccaatcgcat agatgtccgg atcacttgtc tgcagatatt cattgactat cacagtctca
    14041 tcctcataca aagccacttt ttcttttaac aatgtcgtat taggacgagt ttgggcagaa
    14101 aaaatgacca tatctgcctt tattgcttga gatagaagtt gtacggatac tactttctct
    14161 tctgattttt tccaatcgat caagaattca tttggataaa aattgatttg cttagtttgc
    14221 agctgatttt gaaaactgtt gatcatatcc ttatcgaaat atcttgttaa aacattcggc
    14281 atataatcaa ctagatgaat aatgtatcct ttatttctca aagcatctgc taattccaat
    14341 ccaatataac cagcaccgaa gatcactact tcttttgctt cttttaaagc ggcaagtact
    14401 tgcttagatt gcactagatt tttataggtg tagacatttt cagcttcaat ttgtttatac
    14461 aaggtgacag gagaggaacc tgttgctacg attaatttat cataggttat ggtctcgttt
    14521 tttccttctt tggatgcgag gatgagctga tgtttgtctg gatcaagttc agttactctc
    14581 cattcggtga tcagttttgc cccactttct tgaagagtcc gcatgttatg ggcaacttca
    14641 gctaaactac gtatctttcc ttttaaataa aggttgattc catttgaaac atagcctagt
    14701 ccatctttat gatcttcatc gatcagtaaa acttctgctt tcggttgaag ttttcttaga
    14761 tttagggcag ctgtgatgcc tgcatgggaa gcaccaatga tcacgatttt catgttttat
    14821 tcatccttaa attcaatttt tctaaataac attcttttaa aaattggttc aaatagggga
    14881 aatcataatt atattcgttg ctagtgaata caaaaacttt agcggacttc tgtgacgggt
    14941 actctttcaa ttggctagtc aaaatgatat catactgctt ttctttctcg tattcgacta
    15001 aatcatattt gatcgtattc agcaattgtt cctggatgat ttctttcacg atatccgcca
    15061 ttagcctttc ataaggaaga tcataagcaa tttgcagacg taattctaac tgcttgatca
    15121 gcgtccgcaa aatagatgca tagcgagcaa ataattcaaa gcgggcattt tcctcgtcta
    15181 cttcagtaaa atcaacaata gcaaatgttt gtctgaccaa tctttcacat aaaaccagga
    15241 attgtttatc ttctacttca gttaaccgct gttttaatcc ttcttggcca aaaatattca
    15301 tccatcctct aaaataggca aaatgagcgt gattttgcat cactgtcaac gcaatctttt
    15361 gcgaaatctc ttctggaaaa acataaaaag agaaatattc ttctatcgat tgtttaaacg
    15421 ttcgattcaa ttgagctact acgtcgtctt tgtatttttc gcttatcaaa aactcctgga
    15481 catgcgagtt atgtgtatct aatgtaaagt tactgatcat gaaaagataa aaaaccaacg
    15541 tttcattttg attccaagga aaggcatatt gaaagaagta acgggtcaaa aatgtatgga
    15601 ggtccaataa aaattgtgtc gtttgaaaca atccaacagg aagattgcta taatctggtt
    15661 tggatttttt cgtacagcgc attcgcttct tcataatgga aagccatatt tcaaatttca
    15721 atcgtccttc ttctgtaata gtaaaactaa actctttttg cagcagatct aaaaaagtgt
    15781 ttcctgataa atcactgtat tcttttttgt tttcaagata aggacgtcca aaccaaaata
    15841 attggaaata aaaataacaa atttgacttt ctggaccggt cagccgcccg ctttttattt
    15901 tcagatgaaa ctcatttaaa gaatcattta attccttcac tctgcgagac aaagtagccg
    15961 aactgatatt aagttccatg acaagtttct gccgatcaaa tgagccattt tccaacagat
    16021 aaagaagtat ctgatatttt gtagaatagc gcaagtagca atgataataa acatcgactg
    16081 ctgctatatt tccatgtctg attaataaaa tattcccttc ttggataaca atttccaatt
    16141 gtttgatatt ttccaagtta gcttgtaaat aaaacagatc ttctttcaag gtagcccgat
    16201 caactgataa gcgggtaagt acgttattaa cggaaatctc tcctttttcc tctaataaaa
    16261 gttggaaaag tttgatttgc cgttgttcat ttttttcaag caaactgaaa agttccaagt
    16321 ccactcctcc aagcatttct gattttaaca gcagaagttt ccttctcatc cttgaaaaag
    16381 aggatctgtg acagttatca cagatccata caaattctaa tcacttagct ggttggccca
    16441 gctgtcagtt cccatgtgac catgccttga tacgttgcgc caggttgagc attcgtactg
    16501 ctatttacct ctaagagtaa accatcggta tcgggaaagt caacgagaac ctctccttta
    16561 gctgttccat cactctcata aatcaacgtg ttttgacctt gctgaatcaa ttgttttgat
    16621 ccagaatgat tgaagtataa ttttgttccg cttaattctt gaccttgatc attcttaaat
    16681 aaagaagtca atgtccctgt cagttgccaa ggagacgtgc ttggaagccg agtatcccgc
    16741 acatcaaaag aaaaatcgcc ttttgcttca taataacttg tcttcaacgg tttggggaga
    16801 tcgttaccaa atgtaaaatc atctggtaca ttaactaatg ttaattcttg tttggggaca
    16861 agaattgttt tagcagatgt ttggacaact gggttaggaa gcttagcacc atctgctaat
    16921 gtaatattgc tggaaagtac agctgccttt gtcagtatgc tgacggattg tacagaagta
    16981 tctacagtat ttaattgata gttgtaagta agatgaatgg tatctcctat tttgctcaat
    17041 agatctgctg ggacagaaac tccttgttga atgccggtat ctgcgatagt tgtaccattt
    17101 aattttaagg aaccattgat ataagtagta ttttcaggaa gtgagatcgt gatttttttg
    17161 tcggttatcg catccccaga ttttgttaac gtgaatgtca cattttcttg aatcgtatct
    17221 cctgatagag cagacagggg atctcctgtc tcaggttgtc tgctaaggtt ttttagctga
    17281 ctatctgcac tcacagatgg aaaattagcc actgttattt gaggaagagt ggtgattcca
    17341 gcttgttcag actgccattt gtacgcaaaa gacggataca tgttagtaag tacatcgtcc
    17401 accaagcgat aatcaaaata atattgccct gaagccaatg caggtagtgt agaagtagct
    17461 gtatttacac ccccgttatt tgattggatc gtttggaaaa gttgatctgt ccatcctgtc
    17521 gctaattttt gggttccttt cgaataaagc ctgtaatgga tcgaaccctt ttttagttca
    17581 gttgctgacc aattggccgt gacttggtac gatgcagaag aaaggagctt tccccactga
    17641 ctatcggctg ttaagtttgt tggtaaaacc gttccgtcta gcttgtactg ctccaatacc
    17701 tttaaagcaa caggagaaag caatccgcct tgcgcatctt tcccactgta cacgatattg
    17761 gctgtctgat tcaatccact tgtgcttgct ggagtatgag tcagtattgc tccttgtgta
    17821 acgttttttc ctgtaaaata gttgactacg cgatcaaagc gactaaatgg caactgatca
    17881 aaacgtcctt cagtcagccc catcgtcaat gtccgactat tggtagagcc tggaaggttc
    17941 tggttatttc catccacgta ttctgtggta tcagcaaatg tattatattt tcctgttgaa
    18001 tcacttgttg agatgtaact attccacgta tttatctctg cacttgcaga aaatgcatat
    18061 gctcctggat tcaagtttcc gccagttaat ttaggataaa atctacctgc agctggagct
    18121 aaaatcgaat tgaacatacg atcttggata atattattcg aatcataaaa atcaacagaa
    18181 aaaacggcct ttttagacat agaaggttgt aatttaccct tggtcaaaat tttgtttgga
    18241 ctcgtatcat atttatctcc gttttctgtc atcttcgcga cattagctgt aacatcaaat
    18301 tgaagcggga tatcgtccac gttgaattcg cctcgcgtaa taatagcaat aatatctgcc
    18361 aatagagtat ttgtatccca cgaccccatc ccaaaacgca aggtatggct tcctgctgat
    18421 aataaactac cgctagtaac cttgatgggg gcaccggcaa tatacaagta ggcgttgttc
    18481 cagtcaattg cttttaatac atcactgctt gatacatgtt ccggcaaatt gatgatcgcc
    18541 acaaatgcaa atccttgtgc agtgatattc accaaatcaa gtacatttgt tagtttcaca
    18601 tggctttccc aatgaaaacg gaccgtgcca cctttccaag gatcatttga accatcgttc
    18661 aaataagtag cagtcaattt aggcaattgg ttagccccag ttagttggac aagcttcccg
    18721 ccattgactg cactacgtcc accatttgga ggtacgatat tttcatacgt gtatggatca
    18781 taagaaatgg caggaggcgt tgctccaaca gcttctgccg aaggtgaaaa gataaatata
    18841 cttaaaacaa aaagcgaagt aaaaaagaag gagcgaaaaa atgaaaattt attttgtagc
    18901 aacatgttca tcctcctttt cctttcttct ccataacaac aaccacgcaa gaagccataa
    18961 ttctatacca atgatcaaat aaagaaaacg gatcttttct tcattcgtct ccgggaatac
    19021 gccatgttga ttagattctt tataaatcgg tagctgtgtc tgctgttttg atgcgccatt
    19081 gactgaacca cttggatgcg gaactggact tagatcataa aatgtaatcg tacctgaact
    19141 tttggtatct gcttggactg gctcttgcca gctgaagaac agcaaacttg caataatccc
    19201 tcccaataag atcgatgttt tcaacgaaat cacctcatca tgttgtttct ccttttttct
    19261 ttttcttgtg gacccacaaa aacagcaaaa tcaaccataa cactagaact gcagcaacca
    19321 atcctgcata aagccatggg aaagattgtt cttgtactgc aaaaacggaa ttttcattta
    19381 ctttttgtgc tttttctttt ttgatcgtaa agttttgtgt aaatcgccat tcattactgt
    19441 cgcctgattt tgctatgatt tctgcgcggt aatcgccggc ttttgcttta tcaccagcta
    19501 aatcaagatt caagtgaaaa ttcgaatttg gtgcaactaa aaattcatta gacgtatttt
    19561 caatataaag ctgattttca taaaagatct tgctcgtaaa tatcaaattg ctgataatcc
    19621 taggctgagg attttggaaa cgaagctgaa cagttggctt tgcgttgacc tcgtccaaat
    19681 ctcctgataa aagtttcaga tccggttcta tcgtctcttg actttcttgt aaaacgactg
    19741 cgagattata agcaatacga tttttgattt gtgctccttc tgtagcattt tctgtttcag
    19801 cttcttcttt aggtgaaaca tgaatacccg ctaatacacg tcccttaaag ctttttgctg
    19861 ggattttcac ttctataggt atagttgttt ctgcatgtgc tgatacattg atttcctttt
    19921 tcgcaatgag caaaacgtca cggacatcaa acggcaatgt ctcatctaac gttgggtttt
    19981 tttgactata atcgatcgtt ccaccatcac tcgttaccgc cggattgaca gagacctcaa
    20041 atgttcgttc ttcatctgaa gaattgctga taattacttc tagtgtctga ttttgttccg
    20101 gtgccactaa taaatcaaaa taaccgattg ctttatctat ttggttctct ggaaataccg
    20161 gtttgattcc aaagtctcct gctgaatcct ttgcttggct gattgacgga aaaatgaaaa
    20221 atagacttaa aatagaaaaa aacgaaacaa acagtcgttt actttgattc attgtccacc
    20281 acatcctttc aacaacaact gcggtaaaaa atccttcacc tatagtattt tcaccgcagt
    20341 ttgttgaatt tatttcatac tctatgctac tgggccagcc acgaggttcc aagtcaaatc
    20401 agccgtgaat gttccagctg ctgctttcgg cactttcagt gtgatatttg ctggatcata
    20461 aacagaaagc catgttccca gaccttgatc ttttgctgct gtaaatattg gtgtcgctgc
    20521 acttgcttta ctcaacccag ttacagtttg actagttggt ccagtggatt cgttatctga
    20581 cgtactttta acagtggatg ttccaaaatc taattcagcg ccttgcaaaa cttgtgaacc
    20641 gtttacaaaa tctgaaaccg aaactgtcac gttccaccct tgtgcttggc catctgcacc
    20701 tacacctcga cgatctgata cttggagata aggtgtatcg ttttttgaag cgtctacttg
    20761 atacgttttt gttccgcttt caatctcgtg tgtaccaaat ggcaattcag gaactacatc
    20821 caatgttaaa gaacctgtct ggccagttgc tggattttcc ggatctgctg gctctactgg
    20881 ttttgaagga tcagtagggt taagaggctc aacggtattt gttggttcag taaaggtaat
    20941 atctcctttt gaagtcgctt gaccctcagc aaaagctggt gttgccaagc cgcctagaag
    21001 aagtgttgaa agtaataatg tcccaatcat gttctttttc ataataaatc actccttttt
    21061 cttagtgacc taagtatagt agagacagta tgcaaaaaat cctcatgaat tcatccgctt
    21121 acttgaaatt atgacccttt ttgtataaaa gagaaaaaaa caataaaatc gtttacatga
    21181 atattcttcc ttttttatta agtatgaaaa aatatttcaa ttttattttt tcgtttcaaa
    21241 ttattctatt agtgacatcc ttgaactata aaaataggag tacggcatca tctgccgcac
    21301 tcctattttt ttcgtcattt atttcttaaa taaaacgatt ctcacgcaat tttatttttt
    21361 tgatatcttc tactgtttgt gttccagcta attgcatgac catttccaat tctgttttaa
    21421 agaaatcaaa cacttgatgg actcctgtac ttcctcctaa agacaatcca taaataacgg
    21481 gtctgccaat agcaacaaga tcggcacctg atgcgattgc tttgaaaaca tgttgtccac
    21541 gtcgtacacc gctgtcaaac acgattggta cacgtttatc tactgcttct gccacatatt
    21601 gcaacgaatc aaacgcagca ggccccccat ctaattgacg gccaccatgg ttagataccc
    21661 aaattccacc agcaccagat tctaatgatc gatagacatc ttcttctgat tgaacacctt
    21721 tcacgtagac tggcaaatca gaatgagcag cgataaattc tacatctttt ggactaagtt
    21781 tttgtttgga agatttatat acagcatcca tcgtttggcc tactcctgat tgataagcct
    21841 gaacgatcgg cattggcagc gggaaggtaa acccgtttct gcggtctgtt tcacggtttc
    21901 cgccaactgt tgcatccgct gtcaatacga ttgcttttgc tccattacgc ttggccactt
    21961 caagaatatc caaattgatt ccattatctt tgctcatata aaattgaaac cattgcggtg
    22021 ctttctcccc gcctgcttct cttatctctt ctaaagtaca agaagcgtaa gaacttgctg
    22081 tatagatcgt cccaaaatct gctacacctt ttgcagaagc tttctccgcc ttcacatgag
    22141 ctaatccatg agcagcaact ggtgccatga tgattggtgc agtcagcatt tcttcgtcaa
    22201 aatgtgtagt tgtatcgggt aattctacat ctcttaatac atgaggaata atcaaacgat
    22261 gattgaatgc ccgttcattt tcttgataag taaataagtc gccagcacca ctgctgatgt
    22321 aaccataacc accagctgga atcacttgtg aagccgcgat ttctaaatct tccatattga
    22381 taaaatctac tattccttca tttgtacctg cttgatatgt tttttccatc tcaatgcctt
    22441 ctctctataa aatttgaatg atgaacaata tgatagcgtt tacacttttt atattactct
    22501 tcgacattga tacaggcaaa taatctgttt acatttcagc cattccagat gtcactactc
    22561 tggaagaaat gattgtaata aaaatagata aaaaaccgat gggactttga caaaagtagt
    22621 gtcaaagtcc ctttttttga ataaacggtg ttcaaaagcc attttctcgg tattaagtca
    22681 aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttactac tcgaaactaa
    22741 gcgacttttt cacaacctct cgataaaacg gtgttcaatc agctgaccct cggtattagc
    22801 tcaaaaaatg gcaaaatatg aggaactatt ttgccatttg tctctctaat actcaggtca
    22861 gaacgttgac ttcacagtct cttccttaaa cggtgttcaa aaacttgcat ctgcggtaat
    22921 aagcccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga
    22981 tgcagggcag gtttttcacg acctctgatt cacggtgttt ctttttcatc aacattttta
    23041 cgaaatactg tggaagacta ttgaaataag tgtggattgt ttctttgtgt ttcaatcgtt
    23101 tcttgccatt cttcatttaa ttttggatta ccgttttctt ccttgaactc ataaggtcct
    23161 aattcaaaaa tcccagacag tatttgattc acgatttcat atagttcagt gtgatcagac
    23221 acatgattct tgaagatcat ctcattatca ccacgtttga acctcacatt cgggctctca
    23281 tgatgaacct ctatgcattt ttctcctttt tttaactgac ggacttctga aaacatctca
    23341 taaatatctt ctccagtaaa accttcagca agcgccagac tgtccacaag accgcctact
    23401 gtcagttcag agattattgc ttgattggaa tctaatttta atgtatagct tgtcgcttct
    23461 actacgccac atgcattgca accgccttct gatagttcgc tttttctaat caattgaact
    23521 tctttcattt tctcttcctc ctcttaattt tctacttatg tatttttatc acctgacata
    23581 ggtgactcct atcaacgcta tcatgtgctt ttttcctcag aaatcctatt tatccattcc
    23641 atctatccct tattcttcta cttttctttc tctagcatat aagagatacg catagtattc
    23701 aagcaaggat tgtcgatata aaaagaacaa atactgctat tttgatttct tttggctata
    23761 ttacatataa ataaa
//
LOCUS       DAYYGY010000045        23708 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000045 DAYYGY010000000
VERSION     DAYYGY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.046"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACHA52_001670"
     CDS             5..403
                     /locus_tag="ACHA52_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8940368.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACHA52_001671"
     CDS             387..1082
                     /locus_tag="ACHA52_001671"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8940369.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="ACHA52_001672"
     CDS             complement(1676..2563)
                     /locus_tag="ACHA52_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8940370.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="ACHA52_001673"
     CDS             2748..3587
                     /locus_tag="ACHA52_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8940371.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="ACHA52_001674"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="ACHA52_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8940372.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="ACHA52_001675"
     CDS             5055..5411
                     /locus_tag="ACHA52_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940373.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5543..6571
                     /locus_tag="ACHA52_001676"
     CDS             5543..6571
                     /locus_tag="ACHA52_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940374.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="ACHA52_001677"
     CDS             complement(6597..7037)
                     /locus_tag="ACHA52_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8940375.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="ACHA52_001678"
     CDS             7197..7703
                     /locus_tag="ACHA52_001678"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8940376.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="ACHA52_001679"
     CDS             7871..9784
                     /locus_tag="ACHA52_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8940377.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="ACHA52_001680"
     CDS             9789..10790
                     /locus_tag="ACHA52_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8940378.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="ACHA52_001681"
     CDS             complement(10992..11198)
                     /locus_tag="ACHA52_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8940379.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="ACHA52_001682"
     CDS             complement(11844..12692)
                     /locus_tag="ACHA52_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8940380.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12856..13758
                     /gene="gnd"
                     /locus_tag="ACHA52_001683"
     CDS             12856..13758
                     /gene="gnd"
                     /locus_tag="ACHA52_001683"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8940381.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="ACHA52_001684"
     CDS             13760..14761
                     /locus_tag="ACHA52_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8940382.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="ACHA52_001685"
     CDS             14758..15042
                     /locus_tag="ACHA52_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8940383.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="ACHA52_001686"
     CDS             15075..15872
                     /locus_tag="ACHA52_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8940384.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="ACHA52_001687"
     CDS             15886..16719
                     /locus_tag="ACHA52_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8940385.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="ACHA52_001688"
     CDS             16737..17594
                     /locus_tag="ACHA52_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8940386.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="ACHA52_001689"
     CDS             17616..18125
                     /locus_tag="ACHA52_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8940387.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="ACHA52_001690"
     CDS             18162..18563
                     /locus_tag="ACHA52_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940388.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="ACHA52_001691"
     CDS             18675..19028
                     /locus_tag="ACHA52_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940389.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="ACHA52_001692"
     CDS             19062..19505
                     /locus_tag="ACHA52_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8940390.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="ACHA52_001693"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="ACHA52_001693"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8940391.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="ACHA52_001694"
     CDS             20600..21313
                     /locus_tag="ACHA52_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8940392.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21520..21669)
                     /locus_tag="ACHA52_001695"
     CDS             complement(21520..21669)
                     /locus_tag="ACHA52_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940393.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22065..22265
                     /locus_tag="ACHA52_001696"
     CDS             22065..22265
                     /locus_tag="ACHA52_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8940394.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(22369..23670)
                     /locus_tag="ACHA52_001697"
     CDS             complement(22369..23670)
                     /locus_tag="ACHA52_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8940395.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         8089 a         3686 c         4906 g         7027 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct ccttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaatcggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
    21481 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
    21541 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    21601 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    21661 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    21721 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    21781 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    21841 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt atatttcatt
    21901 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    21961 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    22021 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    22081 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    22141 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    22201 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    22261 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa agggctcttt gtcaaatagg
    22321 actgatagag tgagataaga acatctaagg tagaattcct tttctacttt agatgttttt
    22381 tgattcatag gtaatcagat tatttagtaa gaaaattctg gtcttcattt tccgaaagct
    22441 tcgaaagcca taagcgactc gtttcaatgc tttgatgtgg gtatgcagat tttctatttt
    22501 ggcatttgaa taaggcaatt cgagggctaa acgaatgccg gattcatagt ttataaaagc
    22561 tttttttata gaatgcagtt cttttggaac ggaattgggc atcgttctta caagtttaaa
    22621 gaaagtgtcc gcctctttgt cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc
    22681 gattcgtaat tcgtcagaga tcgaaagcag acgatccacc acttcacttt cggtgagata
    22741 cgcccaacca aaattgcgcc atttggtcca tttgtcataa acaagcttgt cctgtcgttt
    22801 gatcagaagc cgagacaacg cctttaattg tcgataatat ttggcttgtg ttggatcgtt
    22861 tcgatcaaag cttttcatga ttcgtacgcg aatatcctca aatttacgaa tgagatgttt
    22921 gacaatgtga aaacgatcca caacgagttt tgcctttgga aaacattcct ttatcagaga
    22981 ggcataagaa acattcatgt ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc
    23041 aaattgcata aaataagaaa cgagagcctt tttcttccgt gaaggaagaa tatccagtaa
    23101 ttttccagta tcgccatcca tacaagaaaa ggagtaggga ccgacttgat ttttcagtga
    23161 acgaaattca tccattaata aacaacgcgg taaccaattt ctttttactt ttgtcatggg
    23221 gatcagagaa gcgagtgtcc gagaaacggt cgaccaagaa acgccgcaat gcttagcaat
    23281 tgtcgtcatt gcttgttttt cagttagctc ttctaaaatt ttatagcgca cttgtttagc
    23341 gataaagcag tttctatcga caatgtaggt attcgccgta aaataggtgt ggcagccacg
    23401 acagtggtag cgttgtttcg ctaaagcaag ataagtcagt tggttcgcac atttattaag
    23461 ttgaatgagc gattttttct ttccgttctt aacgatcatg ccttcatgat tcatctgttt
    23521 acacaaagga cagcgacgaa tttctttact taacgtcgca gaaacaacaa gactcttgat
    23581 attcttaatg ctcttcgatt ccacatttgt gatgaaaatg tttttatctt tgattccaag
    23641 tagtagtttg atatgatgaa gatcaggcat acagaacgct cctttgattg tgttgtggtg
    23701 attcaatt
//
LOCUS       DAYYGY010000046        23681 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000046 DAYYGY010000000
VERSION     DAYYGY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.047"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..321)
                     /locus_tag="ACHA52_001698"
     CDS             complement(16..321)
                     /locus_tag="ACHA52_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8940396.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(346..777)
                     /gene="ruvX"
                     /locus_tag="ACHA52_001699"
     CDS             complement(346..777)
                     /gene="ruvX"
                     /locus_tag="ACHA52_001699"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8940397.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(774..1040)
                     /locus_tag="ACHA52_001700"
     CDS             complement(774..1040)
                     /locus_tag="ACHA52_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8940398.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1295..2020)
                     /locus_tag="ACHA52_001701"
     CDS             complement(1295..2020)
                     /locus_tag="ACHA52_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8940399.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2381..2451)
                     /locus_tag="ACHA52_001702"
     tRNA            complement(2381..2451)
                     /locus_tag="ACHA52_001702"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2417..2419),aa:Gly,seq:ccc)
     gene            2568..3320
                     /locus_tag="ACHA52_001703"
     CDS             2568..3320
                     /locus_tag="ACHA52_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8940400.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3336..3815
                     /locus_tag="ACHA52_001704"
     CDS             3336..3815
                     /locus_tag="ACHA52_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8940401.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4066..5646)
                     /locus_tag="ACHA52_001705"
     CDS             complement(4066..5646)
                     /locus_tag="ACHA52_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8940402.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5639..6382)
                     /locus_tag="ACHA52_001706"
     CDS             complement(5639..6382)
                     /locus_tag="ACHA52_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940403.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6558..7334)
                     /locus_tag="ACHA52_001707"
     CDS             complement(6558..7334)
                     /locus_tag="ACHA52_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8940404.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7582..8418)
                     /locus_tag="ACHA52_001708"
     CDS             complement(7582..8418)
                     /locus_tag="ACHA52_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8940405.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8429..9358)
                     /locus_tag="ACHA52_001709"
     CDS             complement(8429..9358)
                     /locus_tag="ACHA52_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8940406.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9550..9903
                     /locus_tag="ACHA52_001710"
     CDS             9550..9903
                     /locus_tag="ACHA52_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8940407.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9977..10633)
                     /locus_tag="ACHA52_001711"
     CDS             complement(9977..10633)
                     /locus_tag="ACHA52_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8940408.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10859..12793
                     /locus_tag="ACHA52_001712"
     CDS             10859..12793
                     /locus_tag="ACHA52_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8940409.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12879..14102)
                     /locus_tag="ACHA52_001713"
     CDS             complement(12879..14102)
                     /locus_tag="ACHA52_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8940410.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14096..15967)
                     /locus_tag="ACHA52_001714"
     CDS             complement(14096..15967)
                     /locus_tag="ACHA52_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8940411.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(15988..17106)
                     /locus_tag="ACHA52_001715"
     CDS             complement(15988..17106)
                     /locus_tag="ACHA52_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8940412.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17189..18022)
                     /locus_tag="ACHA52_001716"
     CDS             complement(17189..18022)
                     /locus_tag="ACHA52_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940413.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18015..18932)
                     /locus_tag="ACHA52_001717"
     CDS             complement(18015..18932)
                     /locus_tag="ACHA52_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940414.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(18955..19134)
                     /locus_tag="ACHA52_001718"
     CDS             complement(18955..19134)
                     /locus_tag="ACHA52_001718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940415.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(19159..20283)
                     /locus_tag="ACHA52_001719"
     CDS             complement(19159..20283)
                     /locus_tag="ACHA52_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8940416.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20466..21374
                     /locus_tag="ACHA52_001720"
     CDS             20466..21374
                     /locus_tag="ACHA52_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8940417.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21515..23128
                     /locus_tag="ACHA52_001721"
     CDS             21515..23128
                     /locus_tag="ACHA52_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8940418.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7380 a         4629 c         3954 g         7718 t
ORIGIN      
        1 tcctagcttt atagtctatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
       61 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
      121 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
      181 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
      241 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
      301 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
      361 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
      421 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
      481 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
      541 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
      601 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
      661 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
      721 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
      781 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
      841 tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggccgg gtctccagaa
      901 agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa agcccgataa
      961 acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg
     1021 gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt ccagcttacc
     1081 ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc
     1141 gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc tttatcaaaa
     1201 gtactctttt cataaagtat acgctatatt tctttgaatg tatgttttta cacaattttt
     1261 tcataatatt cataaaaaat tcgaatagct gctactaatt ttttgttttt gttgcttgtc
     1321 ctgtctcata attgatcgtg tagccatctt gaacattgta gataaagaca ttgaattcaa
     1381 tttttttgtc ttcaatgctt tctgcttcca attgcactcc cctggctacc agttcattcc
     1441 cctcaaaatg aggagtgaca cggtagcgta catgatgatt tgtttctttg atatatgccg
     1501 caatatcatt ttcgtgagca agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca
     1561 agtttttaat gttgttattt tgaccagtca gctggaaacc aattaaatgg ctgcgattat
     1621 ataaccattg cccgtcactt aatttctttt gtttccaacc tgtcgggtca atatacaaag
     1681 gctctctctt ttcagtaggg aacaaatctt ttccaagcat agcattggct tgtcctactc
     1741 tgttcaattt atcaatattt gaatagcttt cccatgttcc tttggaaagg ctaagatcct
     1801 cttttgtaaa ttccggctga ttcccattga tttctatttc ttgatgtttt ccatcatatt
     1861 ctggcacatc cgctacttga gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct
     1921 gaaagatatc tgaatctttt tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag
     1981 cgacgatccc cacgatcgcc ttggttgttt ttttgctcat ttctttactc ctttactaac
     2041 agctccctaa atcttaacat atgttcccct ataaataaat actagaaaga aatagaaaca
     2101 gcaaaaaata aaacctgttt taagatggct taaaaataca aaaaagagac ggattcctcg
     2161 tctctaatca gtcaccactc tatctgtaag gtatttatta ttattcaaaa attttcttca
     2221 tactcaagac aatacactga tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat
     2281 ctgcggtaat aagtccatcc aagttaaaaa cataagaagt tgtttttgct tggctgttct
     2341 tattacttgt tgcagagcag ctttttcaca acctatttta agcgggtgac gggaatcgaa
     2401 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg caatacaaaa
     2461 acagtatacg cctcttttat tccttcctca agttttttta ttctttacgc ttctggtatg
     2521 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     2581 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     2641 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     2701 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     2761 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     2821 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     2881 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     2941 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     3001 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     3061 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     3121 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     3181 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     3241 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     3301 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     3361 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     3421 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     3481 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     3541 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     3601 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     3661 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     3721 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     3781 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     3841 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     3901 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     3961 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     4021 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     4081 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     4141 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     4201 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     4261 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     4321 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     4381 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     4441 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     4501 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     4561 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     4621 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     4681 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     4741 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     4801 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     4861 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     4921 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     4981 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     5041 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
     5101 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
     5161 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
     5221 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
     5281 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
     5341 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
     5401 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
     5461 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
     5521 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
     5581 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
     5641 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
     5701 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
     5761 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
     5821 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
     5881 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
     5941 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
     6001 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
     6061 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
     6121 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
     6181 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
     6241 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
     6301 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
     6361 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
     6421 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
     6481 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
     6541 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
     6601 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
     6661 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
     6721 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
     6781 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
     6841 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
     6901 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
     6961 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
     7021 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
     7081 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
     7141 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
     7201 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
     7261 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
     7321 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
     7381 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
     7441 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
     7501 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
     7561 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
     7621 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
     7681 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
     7741 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
     7801 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
     7861 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
     7921 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
     7981 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
     8041 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
     8101 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
     8161 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
     8221 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
     8281 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
     8341 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
     8401 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
     8461 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
     8521 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
     8581 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
     8641 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
     8701 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
     8761 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
     8821 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
     8881 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
     8941 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
     9001 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
     9061 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
     9121 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
     9181 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
     9241 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
     9301 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
     9361 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
     9421 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
     9481 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
     9541 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
     9601 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
     9661 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
     9721 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
     9781 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
     9841 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
     9901 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
     9961 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    10021 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    10081 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    10141 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    10201 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    10261 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    10321 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    10381 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    10441 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    10501 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    10561 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    10621 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    10681 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    10741 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    10801 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    10861 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    10921 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    10981 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    11041 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    11101 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    11161 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    11221 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    11281 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    11341 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    11401 acaccctgac attttgattc tagatgaacc aacgaaccat ttagatatcg agacactttc
    11461 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    11521 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    11581 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    11641 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    11701 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    11761 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    11821 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    11881 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    11941 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    12001 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    12061 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    12121 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    12181 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    12241 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    12301 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    12361 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    12421 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    12481 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    12541 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    12601 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    12661 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    12721 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    12781 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    12841 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    12901 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    12961 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    13021 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    13081 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    13141 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    13201 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    13261 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    13321 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    13381 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    13441 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    13501 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    13561 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    13621 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    13681 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    13741 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    13801 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    13861 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    13921 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    13981 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    14041 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    14101 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    14161 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    14221 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    14281 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    14341 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    14401 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    14461 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    14521 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    14581 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    14641 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    14701 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    14761 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    14821 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    14881 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    14941 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    15001 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    15061 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    15121 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    15181 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    15241 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    15301 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    15361 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    15421 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    15481 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    15541 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    15601 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    15661 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    15721 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    15781 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    15841 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    15901 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    15961 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    16021 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    16081 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    16141 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    16201 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    16261 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    16321 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    16381 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    16441 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    16501 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    16561 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    16621 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    16681 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    16741 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    16801 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    16861 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    16921 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    16981 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    17041 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    17101 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    17161 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    17221 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    17281 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    17341 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    17401 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    17461 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    17521 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    17581 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    17641 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    17701 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    17761 tggctgtagc taaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    17821 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    17881 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    17941 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    18001 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    18061 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    18121 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    18181 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    18241 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    18301 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    18361 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    18421 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    18481 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    18541 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    18601 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    18661 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    18721 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    18781 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    18841 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    18901 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    18961 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    19021 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    19081 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    19141 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    19201 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    19261 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    19321 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    19381 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    19441 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    19501 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    19561 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    19621 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    19681 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    19741 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    19801 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    19861 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    19921 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    19981 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    20041 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    20101 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    20161 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    20221 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    20281 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    20341 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    20401 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    20461 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    20521 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    20581 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    20641 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    20701 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    20761 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    20821 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    20881 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    20941 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    21001 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    21061 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    21121 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    21181 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    21241 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    21301 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    21361 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    21421 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    21481 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    21541 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    21601 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    21661 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    21721 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    21781 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    21841 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    21901 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    21961 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    22021 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    22081 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    22141 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    22201 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    22261 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    22321 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    22381 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    22441 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    22501 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    22561 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    22621 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    22681 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    22741 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    22801 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    22861 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    22921 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    22981 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    23041 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    23101 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    23161 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    23221 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    23281 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    23341 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    23401 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    23461 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    23521 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    23581 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    23641 gtattaaata atttaatttt tttgttccta tttatttaac a
//
LOCUS       DAYYGY010000047        23660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000047 DAYYGY010000000
VERSION     DAYYGY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.048"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..352)
                     /locus_tag="ACHA52_001722"
     CDS             complement(77..352)
                     /locus_tag="ACHA52_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940419.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            717..929
                     /locus_tag="ACHA52_001723"
     CDS             717..929
                     /locus_tag="ACHA52_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940420.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            936..1160
                     /locus_tag="ACHA52_001724"
     CDS             936..1160
                     /locus_tag="ACHA52_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940421.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            1166..1720
                     /locus_tag="ACHA52_001725"
     CDS             1166..1720
                     /locus_tag="ACHA52_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFM8940422.1"
                     /translation="MKGGGLNVVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSAR
                     VIGKENLTKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLE
                     KNKVIKHMQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQIN
                     SERSTEEKLDELLEKISGELDGTS"
     gene            1707..2951
                     /locus_tag="ACHA52_001726"
     CDS             1707..2951
                     /locus_tag="ACHA52_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFM8940423.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            2964..4463
                     /locus_tag="ACHA52_001727"
     CDS             2964..4463
                     /locus_tag="ACHA52_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8940424.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            4468..5394
                     /locus_tag="ACHA52_001728"
     CDS             4468..5394
                     /locus_tag="ACHA52_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8940425.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            5521..6123
                     /locus_tag="ACHA52_001729"
     CDS             5521..6123
                     /locus_tag="ACHA52_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8940426.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            6136..7059
                     /locus_tag="ACHA52_001730"
     CDS             6136..7059
                     /locus_tag="ACHA52_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8940427.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            7077..7340
                     /locus_tag="ACHA52_001731"
     CDS             7077..7340
                     /locus_tag="ACHA52_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940428.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            7352..7681
                     /locus_tag="ACHA52_001732"
     CDS             7352..7681
                     /locus_tag="ACHA52_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFM8940429.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            7678..8004
                     /locus_tag="ACHA52_001733"
     CDS             7678..8004
                     /locus_tag="ACHA52_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940430.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            7988..8326
                     /locus_tag="ACHA52_001734"
     CDS             7988..8326
                     /locus_tag="ACHA52_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8940431.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            8326..8718
                     /locus_tag="ACHA52_001735"
     CDS             8326..8718
                     /locus_tag="ACHA52_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFM8940432.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            8729..9241
                     /locus_tag="ACHA52_001736"
     CDS             8729..9241
                     /locus_tag="ACHA52_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFM8940433.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            9287..9655
                     /locus_tag="ACHA52_001737"
     CDS             9287..9655
                     /locus_tag="ACHA52_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFM8940434.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            9709..9996
                     /locus_tag="ACHA52_001738"
     CDS             9709..9996
                     /locus_tag="ACHA52_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940435.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            10013..13132
                     /locus_tag="ACHA52_001739"
     CDS             10013..13132
                     /locus_tag="ACHA52_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8940436.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            13144..13932
                     /locus_tag="ACHA52_001740"
     CDS             13144..13932
                     /locus_tag="ACHA52_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFM8940437.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            13929..16760
                     /locus_tag="ACHA52_001741"
     CDS             13929..16760
                     /locus_tag="ACHA52_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8940438.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            16735..18492
                     /locus_tag="ACHA52_001742"
     CDS             16735..18492
                     /locus_tag="ACHA52_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8940439.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            18578..19291
                     /locus_tag="ACHA52_001743"
     CDS             18578..19291
                     /locus_tag="ACHA52_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940440.1"
                     /translation="MRQAAIHYDGSDHDAHVLATESNAGFATPETVMQARGRWLRDYK
                     LPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSFVEMMVFADHDSRKLIVAFARYSG
                     EIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGELRKGQSVNLPDDTFRYQTLRIHYT
                     DGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEFELTIEARKITISNGRALDISSGN
                     VSNPAMSNNVKITKIEGVK"
     gene            19291..21477
                     /locus_tag="ACHA52_001744"
     CDS             19291..21477
                     /locus_tag="ACHA52_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8940441.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLGDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            21480..21926
                     /locus_tag="ACHA52_001745"
     CDS             21480..21926
                     /locus_tag="ACHA52_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940442.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            21928..22065
                     /locus_tag="ACHA52_001746"
     CDS             21928..22065
                     /locus_tag="ACHA52_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8940443.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            22102..22395
                     /locus_tag="ACHA52_001747"
     CDS             22102..22395
                     /locus_tag="ACHA52_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940444.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            22418..22615
                     /locus_tag="ACHA52_001748"
     CDS             22418..22615
                     /locus_tag="ACHA52_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8940445.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22626..23645
                     /locus_tag="ACHA52_001749"
     CDS             22626..23645
                     /locus_tag="ACHA52_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8940446.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
BASE COUNT         8281 a         3839 c         5114 g         6426 t
ORIGIN      
        1 aaaaggtaag tgtttctaat tcagatattg acggtatttg caattgttta aacagagaaa
       61 aaatatctaa tccttactaa gagaaaatat gttcgttaat gaatttccaa ttttcctctg
      121 tagctctacc ttgataattt ttttgcagtt cacagatgaa taaggaatcg ttattatcaa
      181 gtactgtatt taatttttca cacatttgat ttggattcaa attagtttta actaaccaaa
      241 aagatttttg tagctttata tatgccccaa attcttttat tatttcaaat actttatcat
      301 acttttgccc tgggttatta agatcataac taatcatgta gggtttatac atgttgtctt
      361 gcacatcctt gttttatatt tcagcggacc actcgctgat aaataaaatt atacgcttag
      421 tatttatttt cacaatatta atttgtcact gtggcggaaa gggtagacgc taagcatgtg
      481 tgttaggtca atgcttcggc aaccatgcaa ggttcgattc cttgccagtg actttggcaa
      541 ctgagggttg gaaatgggcg ctcaaagtac acgagcaagg cgaggtcgat agtaatcgat
      601 ggaatcggtg taggttgctt gatagaacta taactgcatc tcgtttatga ggtgtagttt
      661 ttacatatta gatcactcat cgagtggtct ttttattttg gaaggggagt aaaaaaatga
      721 acgaaaacca attaagagag ttgtttaaaa cgaatgaagc aaacaaaaca atggaggcga
      781 cattctacga aactcaaaaa agcttagcgt taatcgcaaa acaagctaag tatttctacg
      841 atcagcttat tctgcaagga tttaatgacg cacaggctat ggaatttatg atgcgaacct
      901 tttctgccag taaccaacag aaagagtgat acgtaatgag gaactactgg tatgtatcat
      961 tatcaaataa atatccgcaa ccaaacgcag atgatccaat tagagttgtc cagtcagtcc
     1021 agattaaaaa gaagtactcc atcattgaaa tgaccagaga agctacgcca aaagagattg
     1081 ataagtacaa tcttcgttac tgcggccatg gatattttag tgagcagaac atacagacaa
     1141 atattgagag atattgttaa taaaattgaa aggtggtggc ttgaatgtgg taaatttgac
     1201 accaaaacaa aaagcttttg cggatgaata tataaaaaat ggtggtaatg ctactcaagc
     1261 cgccattaaa gcaggttata gcaaacgatc agcaagagta ataggtaagg aaaacctaac
     1321 taaacctaac ataatacagt atattaatga acggctaaat cctatcgaaa agaagcgcaa
     1381 attaagtgcc gaggacgctt taaatgaatt gatagatatt tggcaaggag aagtacaaat
     1441 aagcgtgagc aagcaaatag accgcttgga gaaaaacaag gttattaaac atatgcaata
     1501 tgaatataca ccagatttag aaagcaaatt gaaagccttg gatttgtatt tgaagtataa
     1561 atcgctgtta tcacaaacgc aattagaaaa agctcaaaca gaaataaaat taatgcaagc
     1621 aaaattagaa caattacaga taaactcaga gcgttctacc gaagaaaaac ttgatgagtt
     1681 gttagaaaag attagtggtg aattagatgg cactagttga tatttataac ccaaagcaaa
     1741 tcgacgtgtt aaataaaacc attaagaatg attggtttat tactttatta catggagcaa
     1801 aacgttctgg aaaaacaaaa ataaacaatg atttgttctt gtttgaattg cgacgtgtgc
     1861 gaaagattgc cgatgaagaa gacattaagg aaccaatgta tatcctagca ggagtttcaa
     1921 gtgcaacaat ccaaaagaac atcttacagg aactatacaa catgtacagc atagaaccta
     1981 aattcgataa acatggaaac tttaagctat ttggcgttaa ggtcgtacaa gcttatacag
     2041 gaaacattgg cggtgttggc gcaattcgtg gtatgacagc atatggcgct tatatcaatg
     2101 aggcatcgct agctagacaa gaagtatttg ctgaaatcgt ttcacgttgt tcaggaacag
     2161 gagcgagaat actagctgat acaaaccctg acaacccgga acactggcta aagaaagagt
     2221 atatcgacaa ttcaagcaaa aatattcaat cgttccactt tgaattagat gataatacat
     2281 ttttatctga acgatatcgt aacaatatta aagaatcaac accaagcggc atgttttatg
     2341 atcgtgatat caaaggttta tgggtttctg cagaaggtgt cgtttatcgt gattttgatg
     2401 ctagcaagca ctacatccag tcaaaagact taccaccttt gagcaacttt tattgtggtg
     2461 ttgactgggg atatgaacac tggggttcaa tcgtagttat aggtgaaacg gatgacggaa
     2521 cagcttattt aatcgaagaa catgctactc aatttgaaga gattgattat tgggtagatg
     2581 tggcaaaagg cattcaagag cgttacggct tacgagtacc tttttattgt gactctgcga
     2641 gaccggagca tgtggctagg tttgtaagag aaaagattaa tgctaaaaat gctcataaag
     2701 cacggttatc tggagttgag gaagtcgcca agagatttaa acaagataaa ttgtttatct
     2761 gtcaagatag agtgatgaaa tttcgggatg aaatttatca atacatttgg gacaagaaaa
     2821 aaggcgagcc aataaaagaa tatgatgatg tgctagactc cgttcgatat gcgatatata
     2881 ctcatgagct tcttaagaaa ccaaaagtta atgtcaacga aaagattaaa cgtattaaac
     2941 gcatgtttta aggagtgtga aaaatggata aggtaaacga gtttgaatac ggagctgata
     3001 tacattattc taatgacgtg aacacaaatt atgtaaagtt tagcgtagac tcaaatcttc
     3061 actatagatt tagctcagca gaagatttac ttaacgattt agatacttta gcagcaatga
     3121 taaaacatca tcatgaatat caggtaaaaa ggctaagtgt attagatgat tattataaag
     3181 ctagaaatac aaatatcatg gataaccgta ggcgcagaga aaaagaaaaa gcggatcacc
     3241 gatcagcaca taactttgga aaagttcttt gtacgtttga tgttgggtac aacacaggca
     3301 atcctataaa agtgcaaatc gaggacacaa atcaacaaaa agaaatcgaa gagtttaata
     3361 ctaataatga catagatggg ttaaatgctg aactctggct tgatatggat aagtatggga
     3421 gagcctatga gattatctat cgagattcag atgatacaga ttatgttgat ttggctaatg
     3481 tatttgaaac gtttgttgta tatgatacta cagtaaaacg agagcctatt ttggctgtac
     3541 ggtatcctaa gacaagattc aacaaggatg ctgataaaca gtacattcaa ccaatcgtat
     3601 acacaaaaga aaaaagtatc acttatgatg agacgacact aacagcaatt gagttaaaga
     3661 atccccagga tgaaccgcat gaatataaag aggtacctat tacagagtat tctcctaatc
     3721 gttttcggat gggcttgtat gaagatgtac tatctttgat tgatctatac gatgcagggc
     3781 agtctgatac cgccaattat atgactgatc taaacgatgc tcttctagtt attagtggtg
     3841 atattgaagc agcaggacta tccacagagg acgccatcaa gcagaaagaa gcgaatatgc
     3901 ttttgcttga gtctggaact gatgtgaacg gtaataaaac aagtgtgact gcaggatata
     3961 tttacaaaca atatgatgtg aacggtgtag aagcatacaa atacagagta cgcaaggata
     4021 ttcacgaaat atccatggtt cctgatctta ctgatgacaa tttttccgga gtgcaatcgg
     4081 gagaagcaat gaaatataaa ttatttggat ttgaacaaat gacggcaaca aagcaaaggc
     4141 tattcaaaaa aggccttatg cggcgttatc gtcttttatt tagcctaaaa tcaagtattt
     4201 ctgaaatgga taactccgat ttgaaaggct tacgtgtaat atttacgcct aatctaccta
     4261 aagccattct ggaagagttg aaatctttgg ttgatgctgg agctgaactc agtcaagaga
     4321 cgatcttagg actcgcttct tttgttccag atgtacaggc agagttgaaa cgagtaaata
     4381 aagaaacgca aaagcagatt ggcatttttg attcagatgg tgaagaagta attaacaaca
     4441 aaaaagatga aacagggggg tgattaaatg aactcccaag aatattggat caaacgggaa
     4501 aaggaatggc aaaagcaaca aattaaagat gataaaaagc gcatggcaga aattaaaagt
     4561 cgcatgcaat acgcacaaga tgcgatacaa aaagaaatag acgcgcagtg ggacagtttc
     4621 tccaatggcc agaaaatcac tcgtagcgaa gcgatgaagc gtgctagtga aatggatgtc
     4681 aaagcattcg ctcgcaaagc aaagaagtat gtcaaagaga aagatttttc tcctacagca
     4741 aaccaagaat taaagctata caatcttacg atgcgtgtaa atagattaga gctcttaaaa
     4801 gctaatatcg ggcttgaatt gatttcactg tttaatgaat tggataagta cttttcgaat
     4861 gaattaacaa aagctggttt agctgaattg aagagacaag ccggtatttt agaaatgact
     4921 attgcttcaa gtggatatgc aaagctgata gaactagtaa taaacagctc ctttttgagt
     4981 gatgacgtgt cttttagtga tcgcttatgg atgtatcaat ctgaattgaa atcagaatta
     5041 gataggttgt tagtcagaag tataacgatg gggaaaaatc ccaagcaact tgcatctaaa
     5101 ttggcagaat atttaacagc tgaaggacga gaaaacacta agttcaacac tcaacgtttg
     5161 atggtgactg aaacgactag agttcaggta gggattcaag aacgaagtta tagagatgca
     5221 ggcattaccc agtacatcta tatagcagaa ccaacggcgt gcaaactatg tataccgtta
     5281 aataatcaag tttttgatgt tgccgatatg cagccaggaa gtaacgctcc taatatgcat
     5341 ccattttgtc gatgcagtac agcaccttat atagaacgaa tatcaagccg ttaacacaaa
     5401 ttaacggctt tttattgtgc cttcttacag cttacaggcg ttaaagagaa agctattttc
     5461 ggctgaccgg cgtaactggt caaatttatc gggtagcggc gtaaccgtgg aggattaatc
     5521 atgaaaaaac gtttatttat gccaatgaac ttacaatttt tttctgaacc aggagatggt
     5581 ggatctggtg atgagggaca acaaggaaac ctaccagctg gctcacaaga gacaccgccc
     5641 gaagcaaaag aagaaaacaa tactggcaaa acattttctc gtgatgaagt agcgaaaatg
     5701 atcgctgctg agacgaataa agcaaaagca gcgtgggaaa aagaactagc agcaaaaaaa
     5761 gaagaagcta aaaagctggc aaaaatgaat gcggaagaaa aactacagca tgagttggag
     5821 caaaaagaag ctgaaatcgc tgaattaaag cgtggacagg cactatctga aatgacgaaa
     5881 gaagcttcta aaatgctgac agatgcaaat ttaccacacg atgatgattt acttggtctg
     5941 attgtttctg atgatgcaga tgccacaaaa caagctgtag cagtcatcac taactttgct
     6001 tctttgatta agagagaaaa cgcaagacaa acaccaccaa atgaaggtgg acaatttaca
     6061 gcatcgaaaa atactaaaga aacagtggct aaactagctg ctaaaaatcg aattatcaaa
     6121 taggaggaaa acttaatgaa aaagaaacaa cttttaccaa tgaacttgca aatgtttgct
     6181 caaacatggg atccagataa tgtcttggta tatgaaacga aagagggaaa aattcctgat
     6241 aaatataata cgctcatttt gagtgaagtt atggaaaatt ctaagatcat gcagttagca
     6301 aaatacgaag aaatgactga caaagaaaag aaatttgaat actttgcaga aggaccaggc
     6361 gcatactggg tgggtgaagg tgaaaaaatt aaaacgtcta aacctaaatg gatgcaagct
     6421 acgatgactg caaaaaaact cggtgtcatt cttccggttt ctcgtgaata tttaaattat
     6481 aaattatcag atttctttga ggagatgcag ccaaaaattg ctgaagcttt ctataaaaaa
     6541 tttgatgcag ctgccttatt aaataaagaa aacccatttc ctcagtcact agacggatca
     6601 gttattagtg cggggaatgt ggttgaaggc ggattgactt atgataatat cctagcctta
     6661 gaagacaagt tagcagaaaa tgaattcgaa cctaatgcgt ttatttcaaa ccgaaaaaat
     6721 cgtacagaat tacgttctgc agctcaaaca gtcgggtcaa atgttgagtt tatttatgat
     6781 cgctctacta atacaattga cggattacca gtagtagacc ttaagtcttt agataaagga
     6841 acactttacg ctggagactt taattacatg ttttatggga tcccatataa tatttcattt
     6901 aagatttctg aagaagccca attgtctact ttaactaatg aagatggaac cccagttaac
     6961 ttgtttgagc aagaactgat tgctttgcgt gcaacaatgg atgttggatt tatgattgta
     7021 aaagatgaag catttgggaa gatttcccca aaagcgtaac gcctgctacc ggtattgtgc
     7081 caaatcaaaa gacatggacc ggtaaagtag gcgatactaa aacatttact atttcagctg
     7141 tgcctgcaga tgctagcgat gcagctgctg ttgttgcagc tactacagca acttcaagtg
     7201 atggagctat cgcaacagtg accaaaaatg aaaatggtgg ttttgatgga acgattgcag
     7261 cagaagggtc agcaacattc acatttactt ctggagaatt cactacttca atcaatgtga
     7321 caggtcaacc tgctagttag gaagtaaaaa tatgatgatt gcagatgata ttaaaaaact
     7381 tcttaaagga acaatagatg aaaagcttga agttattgag cgaagaacga atgagcgtat
     7441 gaaaaccttg ttaaatacgc aagaagttcc taaagaattt gaaacagttg tatatgaagt
     7501 atcgttgaaa agattcaata gaattggtca agaaggtatg cagtcatatt ctcaagaagg
     7561 tttatctatg gcttttcctg actcggattt ttcagagtat caaaatgaga ttgatgaatt
     7621 taagcgtaaa gatcaggaag agttgtacaa gccaaagcga gggaggttta aatttgtatg
     7681 agatttacag atgaaattat atttgttaaa cgttcatctg actctaaata tgatccagat
     7741 ctcggtgagt gggttgaagg caaaccagaa agaacaagaa cagaggcaaa cgtgacagat
     7801 attggcactg atagaagtgt gactattttt ggtagtgtgg aagaaggggc gaaggtcatt
     7861 aggacgcagc ctcttttttc tatccctaca tttgactata tcgagattga aggaaagact
     7921 tggcaacaaa aaacagctag aaatccagca tatagaaata gtttaattgt gcaagaggtg
     7981 gttcttgatg aaggcacaac ttgaatataa aggaatcgat cagctgatgc gacatctgaa
     8041 aaaagcagca acgcttaatg acgttcaaaa agtcgtgaaa agtaatactg ctgaaatgac
     8101 tgaacgaatg caaaaaggtg cgccagtgga tacaggttac ttacgaagat caataaacat
     8161 gaatctttta gaagctggtt taactggtat tgtaggaccg acagcagatt atgcacctta
     8221 tgtagaatat ggcactcgat ttatgtcggc acagccttat gttagaccag cgtttaatta
     8281 ccaaaaagtc aaatttatgg ctgaaatgaa agccttggtg aaatgatgat taagacaaga
     8341 gatcaatcaa tttttgatga actttttaaa atatcccaaa acaaacttgg atataaaaca
     8401 tacgattaca aaactttaga ggatgttggt tatccttttg tggaatttga gaacactcag
     8461 acaatccatg aagtaaataa aactgacatt aaagggtctg tgattgtggt tttatccgtt
     8521 tggggattac agaagaaacg aaagcagttg tcagatatgg catctgctct ttttaatgaa
     8581 gctagattga tagaagccac agaaggctat tattgggctt taaattatca agcaagtgga
     8641 attcaagtga tggacgacac aacaacgaat acaccgctaa aacgggcggt tgtcacactt
     8701 gaatttagaa ttagatagga ggaagaacat ggaagcatta aaaggtattg atgtcatttt
     8761 gctttatcgc ttattgaaaa aagaaactca ggaagccgct tggaaaatgg catttcaaac
     8821 agaacacgaa aatggtttat caagagattc agactctaca gtgacaaaag atggaaacgt
     8881 tcaaagttta agcccggttg aatatgattt ttcggctact tcaatagttg ctaaaggaga
     8941 ttctcatgta gatgaaatga aacaagcttt attaaatggg gatatcattg aaatttggga
     9001 aattaataaa gcagaacagg gaacagatga taatgcaaat aagtacaaag ctacttatta
     9061 ccaagcatat gtgtctgaat ttactccatc ggctgctgca gaggataacg ttgaattaag
     9121 tttatcattt gcagtaaatg gtgttggtca agatggttat gcaaccttga cagaagatca
     9181 agccgatgtt gttcaatatg cattcaaaga taccgtgaaa gcaacttcga caggagcata
     9241 agagggctta gatgctctct tttttatttt aggaggatga aaaacattga aattaaaaat
     9301 taaaggtaaa gaatattcgt ttaaatttgg cactaaattt gtacgtgaat tagataaagt
     9361 gatgcctttc gtcgatggaa atatggaatt tggaatggga ctctccgcaa aagtcttacc
     9421 agagctacgt tcttataacg ttaacacgtt atcacgagtt ctggaaatag caaatagaac
     9481 tgaagatgaa tctattacat tagatgaatt ggatgattac atcgatgaag ttaaagacat
     9541 tgaaaaattg tttgatgaag tcctaaaaga attggcggag tcgaacgcgg gaaagttagc
     9601 ggtccgaaac ctgaatcaga aattgaaaga agcggaaaaa caacaagtgg aatagattct
     9661 gcactggcat acgaacaaat tcttatcaat tcttttcgat atttgggaat gaccaatatc
     9721 tcagatatcg aaagaatgac gttatatgaa tacaacattc gtatgactgc agcccagtta
     9781 tcttggcttg acaaagaaaa gttgattcac gaattagcgt gggcaaatca gcaagtccaa
     9841 gcggagaaaa aagtaggcaa aaagacagtt cctgtatatc gatcctttga agaattcttc
     9901 aattatcaaa aaatcgaaga ttcaatcatg ggagtttccg aactttcaaa acaagataaa
     9961 aaattccaaa gcttactaac taaagctaac tcttgaggaa aggaggagaa tcatggaaca
    10021 attttctgtt gaagccttat taaaagccac agatagtgga tttgtaaaga cttttaaaga
    10081 tgcacaagat gctgttaaaa cttttgaaaa gaattcaaat agtatgacaa ccgctgttgg
    10141 taaagtgatg caaggtactg gtgccgcaat gacaaagtat attaccactc ctcttatagg
    10201 agtaggcgta gcagctgcta aagttggtgg cgacttcgaa gcacaaatga gtcgtgtaaa
    10261 agctatatca ggagcaaccg gcgacacatt cgaacagatg aaacagcaag cgattgatct
    10321 aggagcaaaa actgctttta gcgcaaaaga atcagctgct ggaatggaaa acttagcttc
    10381 tgctggattt agcgcacaag aaatcatgaa agcaatgccg ggtcttttag acttagcagc
    10441 tgtatctgga ggggatgtgg ctctagcttc tgaaaatact gctactgctt tgagaggatt
    10501 tggtttagaa gcaagtgaag caggacatgt cgctgatgta tttgctcgtg ctgctgcgga
    10561 taccaatgct gaagttggag acatgggaga agcattgaag tatgttgctc ctgtagctaa
    10621 ttcaatgggg atttctttgg aagaaactgc agcagctatt ggtattatga gtgacgcagg
    10681 cattaagggt tctcaagcag gtacaacgtt gcgaggagca ttgtctaggt tagcaaggcc
    10741 aacaaaggct atgcaagata caatggataa tttaggtgtt tcgttttatg atgctgacgg
    10801 taaaatgaaa cctttaaaaa ctcaagtaga attacttaaa aaagcttttg aaggcctgac
    10861 gcctgaacaa caacaaaatg ctttagtaac actatatggg caagaatcat tatcagggat
    10921 gatggctttg attgataaag gacctgattc attgggcaaa ttaacaaaat ctctgaaaga
    10981 ttctgatggt gcagctgacg atatggctcg gaccatgcaa gataatatga attcttccat
    11041 cgagcaaatg tttggagctt ttgagtcagc agctattgta attcaaaaga ttctagcacc
    11101 atccatcaaa aaagtagcag atgccatatc cggcttagta gaaaaatttg taagtgctcc
    11161 agaatcaact caaaaattag tagtggccat aggagcaatt gtcgctgcta taggaccgtt
    11221 aatttttatg attggttcag taattatatg gatcaatagg gtgaaagtag cttttaaagc
    11281 tttaagtgaa agttcaaaat tgtttagtgg attaagtaaa gcaatgggtc ttcttacaaa
    11341 tccggttttt ctggttatag ctgcggtagc actactcgtt gtaggtttca tctatctttg
    11401 gaatacgagt gaagatttta gaaacttttg gattggctta tgggagggaa tcaagtctgc
    11461 tgtaagctcg gcagtagaat ggattcagaa tgcatggaaa tctacaggag aatggtttaa
    11521 caatttatgg aagtccatta aagaaggcgc agacaatgtt tggactacag ttcaagaagc
    11581 ccctgggaaa gcggcagatt ggattaaaaa caaatggact ggaacaaaag agttcttttc
    11641 aaatttatgg tcaagtattg caaactctgc ttcagagatt tggaatagtt taaaagaagg
    11701 tgtcatatca gttattgatg atttagtttc aagtgctggt gaaaaatggg aagggtttaa
    11761 aaatactata tctactgcat ggaaaacaat tacaagtaaa atcaaatctg gttttgattt
    11821 tatactaaaa tatattggtc catttgtaag tagcttttca gatgtgttct ctaatatagt
    11881 gaaagcaata acaagtatat ttgctgaggt taaaaacata atagtaaatg cttgggaaat
    11941 cattaagtct ttaatagctg cgccactact gtttattata gatttaatta ctggtgactt
    12001 cgaacaaatg aaagaggatt tagatctaat ctggaacaca cttgtccaat cagtggtaaa
    12061 tatttggaca tctgtaaaaa atatatttac ggaatatatc ggtgcaatag taaatagtgc
    12121 cgttagttta tggactggat tcatacaaag tatttctaat atttggaatg aagtagttta
    12181 tcaagcgact atgatttgga ttgatttgaa actatttttt actaatttat ggattgatat
    12241 taaatacagt gcaattcaaa tgtggataaa tctaaaattc tccataattc aaacttggat
    12301 tgatacaaaa tatggtgcaa ttgaactttg gaataatcta aaacaatggt ttttccaaac
    12361 ggttaataat atcgtgcaaa ctcttataaa aagttggaac agcttaaagc aaggaacgat
    12421 agatttattc aacaatacag ttcaaggtgc taaagacatt tggacttcat tcaaatcttg
    12481 gattggtgat ttaattactg gaaccaaaga taacatcatt cagggatggg aaaacctaaa
    12541 acaaggtact atagatactt tcaacaattt agtaaatggt gctcaagagg catgggataa
    12601 tttagtaaat gctgttagtg acacggttga tagagtaact ggctggtttg ataacttgaa
    12661 aaatatcgat ttactagcag ccggaaaagc tatcatggat agttttctag aagggttaca
    12721 aaatgcatgg aaatctgtgc aagattttgt tggaggtatt ggtgattgga ttcgtgaaca
    12781 caaaggacct atccaatacg atagaaagct attgattcca gctggtcagg ctattatgaa
    12841 cggtctgaat aaaggtctga caggaggatt caatgacgta cagaatactg ttggaagtat
    12901 ggcagacttt atcgcggaac ttttcaatgc aaattctgat gtagatatag ctgcaaattt
    12961 gaaaaatgca aataaaaaca ttggtgcaca agttgaacat aaaataaata tgggtggctc
    13021 tactaaacca gctgtattta aaatcaatct tggaagacaa tcgtttagat tgtttatgga
    13081 cgatatttca caagctatgg gcgaaggtgc agatattaat ctggaatttt aggagggaat
    13141 attttggatc aacgagaaaa taaaatgtac tcattcaaag atacaaccat taatctcact
    13201 agttctaaac gattccttcc gacgtctgcc atgatgtacg atggaatgta tttagaagat
    13261 ttgattgatg gttatcaaac acttacggtg gaaggtagag aaatgctttc tgtagaagtt
    13321 gaacagcaag agatacaaat tggttcaatc attacaaatc agaaaatacc ttcaagaaca
    13381 ctaaaaataa catataagct ggaagataga gatccagaaa aactacagtt taaattcaaa
    13441 gaactgttga attatttata ccggaatgaa gacgtggaaa ttaggtttca tgatgaatta
    13501 gattattatt actacggtcg ctatacatca actgatactg ttccaggaga ctccaactcg
    13561 attatttcga gttttaatgt attctgtgcg gatccactaa agtatacgaa agagtgtgtt
    13621 agtgatggct atattggaaa tccgatacag tttcctataa caccaagaaa aattgaagtt
    13681 actttatcca tgaataattc aatcaaaatt acaaacggag aacaaaatat cacgataact
    13741 gacgcggcaa taaaaacagg agacgtgttg gtttttgatt tttccgatga gcaggtaact
    13801 gtaaacggag aagattgtac ttctatgatt gatttagaaa gtgattttga gaacttttat
    13861 cttaagcaag gtcagaagat aactagcaat aatgggaagc ttaaaatatt ctataggggg
    13921 gcgacaattt gagtgagaca gtttatttct ttgatcactt gcaaaaactt attaaaagaa
    13981 aaaatacaag aagtttgatt gaagtctccc aagaaaaaga aattagttct gataagagtg
    14041 atctaatgaa agatactctt tacgttacga caaaatatga taaagaaata gaggatgcaa
    14101 gatatatggc gattcgtgaa aacgagtcgt ctttttcgtt gtatcgaatt actaaagtta
    14161 gcgacccatt tgaaacatta gagtttacag ggttaggatt tgcgacaaat gaattagatg
    14221 cttacatcat caaagatatt aggccgagtg ggcagccctt aaaaaatgtt cttgatcgat
    14281 tgattgaatt tactgaagga aattggcgcg ttggtcacgt agaagcaatg ttaccaacag
    14341 taactgcaac tttttactat gtctctgtaa aagaagcgtt gaaagaattg caaaccttag
    14401 gtatggaatt tgtctttagg tgttctttga attctgatgg aataaaggat aaatggatcg
    14461 aagtatatga acaaattggc gaagaatcga atacacgttt cgtatatggt agtaaagcat
    14521 taacagttgt aagagagata gatagaagct caatctcaac ttcaatgata ggtcgtgggc
    14581 gaggcgaaga ggttggtgac ggttacggta gaagaattga attcactgat gttgaatgga
    14641 aaaagtcgaa tggtgatcct ttagataagc ctaaaggcca aaattggctt gaagatccgg
    14701 aatcaactca aaagtatggg ataccacaaa aagatggatc aatgagaaaa cgagaaaccg
    14761 tagtagtgtt tgatgatata gatgatccaa cagatttact taaaaatact tattcaacct
    14821 taatcgattc tgctagaccg ttagtacaat tcaaagctga agtcactgga ggagatgtga
    14881 taggaaatac agtgactatt cacagatacg ataaaggtta tcactataaa actcgtattt
    14941 ataaaactac attcaatcgg cttaccggtc aaacgaatat cgaactaggg gataatttaa
    15001 cacaagatgt tagaaaacaa acggcttcta ttgtcaataa tattaatagt ttagaatcta
    15061 gcaaaatgac attttacgaa tcgacagaga ttggaaaata tcaagatgac attatgcgag
    15121 gtgcaggaga taatggcggt tctatttatt gggtaaatgg aattgaagct ggtgttagtg
    15181 atagtagaga aatctatgaa actgtttata tggatggacc taacattcct agatcacgct
    15241 tttttatggt ccaaaataac tcagggatat ctttcaaaca atgtaaaaaa ggtgagtggc
    15301 aaacaattca agatgtacac aatggtgata gcacgaccgc ttggacgttg gatggaactt
    15361 tcaatgctaa ttttattcgc gcaggcattc tgtctggtat cctcgtgcaa ggggtagctt
    15421 taaaaacatt tgacgataaa gatttccaat tagtggcaga ggggggagaa ctttcttttg
    15481 aaaaacaagt caagtctact ggactagatg atgtacatgg agaacgcttg ggctcaattg
    15541 tatctactta tggttctaaa gggattaacg gctttgctgt ctggaaagag ccaaactata
    15601 ttttttccat taatgctggg gacggtggcg atcgagggaa tcctattttt caaattccag
    15661 cagatgttac tgctgataag cgcaaatata atctttatgg tgacggcaat ttttcagaag
    15721 gaaatataac catagatgga cggctagatg taaaagaact ctatgttaac ggtacaaaaa
    15781 ttgacactaa tggaggaggc aattccggag ggaacgataa cggttggaac ggacaatacc
    15841 caccagaagt aactactgat cgggataaac gatattggca aatttgggca acggcaatag
    15901 gtgccggttt tacacaacaa gctgctgcag ccttacttgg aaatgcacaa ggagaatcag
    15961 atgctaatcc aaccgctgat gagggcaatg gcgcaccagg gttcggttat ggtgtatggc
    16021 aatggaccga ttcttctggc gcaactagcg gacgtgtcta catgatcaat ttaatgacaa
    16081 aggctggcat cagtgatgat ccagacacaa tcacggcaca gtttaaattg ttgatgtggc
    16141 atgcaccaaa tggtcaatgg atcgcaacta gcgcttatcc ttacacatgg acacaattca
    16201 tgaatctgac cgatatcaac acagcagcac aagcattcgt ggctaacttt gaacgtccac
    16261 gtgatccaca tccagaacgg acggcatggt cacaagaatg gtatgacaag ttcaaagatt
    16321 tggaaattcc tgtatcaaaa ggatatatga agccgattgc ggatccaatc acagtgacga
    16381 gcgaatttgg ctggcgcact tctccaatca caggagcaca agaatttcat aacggtattg
    16441 accttgtaaa tggaaatcct aatacaccta tttttgcttc agcggatggc gaagtgattg
    16501 ttgcaggtga tgcaaattac tttgactggt atggaaattg gacagtgatt aaacacgctg
    16561 atggaatgta tacaggatac gcgcatcaaa gccgagtaga cgtctcaaaa ggtcagaagg
    16621 taactgctgg tcagcaaatt ggactgatgg ggacaacagg accatccact ggagaacatc
    16681 ttcattttca atttatggat gagttttatc catcttcttc aggtcatttc cacaatgcaa
    16741 gagactacat cgatttctaa aggagggata gtcatggcag aaacgcagca taaaatggtc
    16801 ctatccacca ccgaaccaaa caacggaata aatttggttc gaattcggca aggggatgtt
    16861 ttaacgcaaa agttcgttgt tgaagtggtg gaacatggca aactaaaaac attcgagggc
    16921 ctagtgccgt tttttattaa tacaacaaaa tttggcgaaa accaacctgt tgaacaaaaa
    16981 gtacaagaat acagtccagc gcaagcaagg cttgtttaca ccttaagtga gcctgactgg
    17041 caatggggtg gtgaaaacac cgcacatttc agtttccgat cacttaatgg tgatggaact
    17101 tggagtgaac aatttagcac acaggatttt acctatcgag tcatttctgg aatatctaga
    17161 agccagttac gtgactctgg ctatgtgtgg acatttgagg atttgctaag aaaattcaaa
    17221 gattacatgg atcagggcaa aaatgactgg gagcagtggt tagaagataa tcgtgaaata
    17281 ctggaaaata tcgatccagg tggtatgatc attaacattc tgaatgaagc taaaggcgac
    17341 tacgaatcat tagctgatcg tttaaaccaa aaataccaag tgccagtcgg cagctcacaa
    17401 attagagaaa caacacgctt ttttgattac gacacgatga aatacgttga cctagtgccg
    17461 cgcaatttga atacggttgt caacagtgtt aataacagca aatttaactt ttctttcatt
    17521 acagacattc acgcagataa tcacaacttg cgtatagatg gcgtcggtta caaagatgct
    17581 tcttatttaa gacattggcg cgcaatccct caatttcaaa aattagggaa caaaacagat
    17641 gtgatgattt acggcggaga caatatcgac ggcggacttg gttcactcgg cagcgatata
    17701 ggcattattg atgaatggag cgcgcgacat tccatgttag gcacgctcaa acgcttcact
    17761 aacgcagcgg tagcaggaca agaaaaaccg gttattatct gcaaagggaa tcacgacgct
    17821 tgtttcgacc ccgcgtggcg caagcgaaag ggaatgttat gcaacgctga cttcgaagag
    17881 tattggaacg gtttgtacgg tggcgtgttg ttcccagata aaaacgcagc aatttaccgt
    17941 ttcgatactt gtgattttta tgaaggcggc acaggcgaca agtacgtgga cggttacagc
    18001 gatacagcgc cgggagcttt cagtgccaaa caaattaacg ctttcggaga atggttagtg
    18061 aacgttccga gaaactatca tgtggcgtta gtaggacata cgcctttagg tctcagcaag
    18121 tttcccgttc gcaatgaaaa catgattagc acgttgatag aagggtttaa atctggtagt
    18181 ccggtaacta tcgattggtc taaattagga caaccgaatg acggttcgtt cggcggatta
    18241 aagaccttcg caatgaatac aaaaggcgcc ggcgttgtag taggttattt ctgcgggcat
    18301 tggcacgaac aagtcgaagg ggcgtttgga acagtaaaaa tgattctttg cgacgtcggc
    18361 ttctctcata cagctagcca agttgataca ccggatgaat tagcgtttta taaaatagaa
    18421 gttgacacag caacaagaaa agtgacaagc aaaggcgtag ggcgcgcgag agacttcact
    18481 tataactatt aaggcggtga acaattaaat gttagatttt caaagcaaac caaatatttt
    18541 tgaagaaatg agttatgaag aggctgtgaa atggttattg cgtcaagcgg ctatccatta
    18601 cgacggctcg gatcacgacg cgcacgtcct agctactgaa agcaacgcgg gctttgctac
    18661 accggaaaca gttatgcaag cgcgtgggcg ttggttacgc gattataagc taccacaaaa
    18721 atatccgaac attttagaca ttccgcccgg caaatacgca accaaagccg gatggggcgc
    18781 agacaaccct gacgggattg aagatgacag ttttgttgaa atgatggtat tcgcggatca
    18841 cgattcgaga aaattaatcg tcgcttttgc tcgttatagc ggcgaaattt acatcaaaat
    18901 gacacataac agcgaaccgg tagagggtta taactcgtta ggctggcggc gcgtttacac
    18961 tacttctgtt ctttttgaag gcgaattaag aaaaggacaa tcagtcaatc tgccagatga
    19021 tactttccgt taccaaacgc ttcgtatcca ctacacagac ggtgacggag actttgtaga
    19081 agaagtgaaa cgtcaaagat acgcgcgaat tacaaaagcg aatttatgga atggagacgc
    19141 gggagtgacg ttcatcgaat ttgagctgac aatcgaagcg cgaaagataa cgatttcaaa
    19201 cggcagagct ttagatattt catccggcaa tgtttccaat cccgcaatga gcaacaacgt
    19261 gaaaataact aagattgaag gtgtgaaata atggcgcatg tcatcaaaaa aggctctatc
    19321 aaagtaccta cacagccgaa agactacgat ttgcaagcaa cggggcttgt atttaaatca
    19381 tacgataatc aaatagcgtt agagttcaac gtcgaacaac agaacggcac accggcggac
    19441 ttgctaggag ctaacttgcg cttgttgatg tttatctatg atgaagtaga tggaatgatc
    19501 acgaaagagc caatcccttt tatcacgaaa aacctcatca ctgaaagctt cttgaatgga
    19561 caggtcgtat atatcttgcc agaagcgatg aaagcttata acggtatggt ggaagcttat
    19621 gtttacatcg agtatccaga cggatcaaca agtgataact taggcttcac cttccgtatg
    19681 aagcgttcag caatcgacgg actagcgcaa gataaagcag actactttat tgcagacttt
    19741 caacaattac ttgatggagt caaacaagaa gcaacagatg ctgttaacgc agcattaaca
    19801 aatgtggatt ctacgattga aaaggcaaac caacaaataa atgaatttgt agagggagcc
    19861 acacaagcaa ttgatcaaac tgttgacgag gtaacagagc aactacaagc tactcaaact
    19921 aagattgata ccgtttctca aaacgttaca tcggcacaaa acaatcttaa agcagttgaa
    19981 gacaagatga atcaaaccaa tcagcaaatc ggcgacctcg gcaagctgaa aaagatgtac
    20041 agtaacagca tcgacttcgg gaattatgat tatagtggga atccgaattt aatgtccaaa
    20101 ctaaaatcga gcgattttaa cgttggttac cacgggtcac taacttcgga taacgaaaag
    20161 ctacatttta cttctgatgg tacaggaagc attattatgt ttacgcgtat taatacacct
    20221 cagcttgcta gtgggaaaac ctatactctg agtgcgaaag ttcgatttga tgaaggaact
    20281 acaggagcta ttgataaatt acgtttggtg tatcgtacat caccaggaga aaagatatta
    20341 ttggaagcaa atagtacaaa tattacaaca gatgatgtag ggaaagaaat aacaatcaaa
    20401 ggtacagcta acgttaatta tcaaatcaca aatttagatc gattttatat gagtattagc
    20461 tttgttgaca gggataaaat aaatggcgga tttaagttgt acgacatcaa aatcgaagaa
    20521 ggctcaacag ccaccccata tcagccaaac ttacttgatg acccttactg gctaggtaaa
    20581 gcgcctttgg gtgagaatat tgctaataag tctgttacgt ttccaatcaa atctagcgcc
    20641 tacagcctat accaagctaa catggaagaa gaatttgttt taggtcaaac ctatacaatt
    20701 accatgaagg caaccaaacc acctatccaa acgttcattg tttacaacga ggatagtgga
    20761 gattatagat atggtaactt agagccagta gaagggttag ttgatacatg gggtctgact
    20821 tttacaccgc agaaggttgg agttaactac cccaaacggt taactattat ccagtatccc
    20881 caatcaacaa caggtgcatg tcaaattgac tggctcaaga tcgaaaaagg cgacacacga
    20941 accccgaata ttagtcagtt taaatacttt ggtgaaggct tgaaagacag taacaatccc
    21001 aatgattaca gttgggatgt cacacctgaa tatactgaaa aaggcttgaa tgatgctgtt
    21061 aatgtgtatg atcctcagag agttgaaggt ttgaaaaact ttgccgatgg tattcaaata
    21121 gcaggagata aagtgatcag tgaaaatgat tgcactgttt atacattaac taaagacaac
    21181 agtcaatcgt ttatcgatgg gtatgcaaca tttattaaac acggaaaaga agttattgta
    21241 aatggtacag taaagttcaa aaaagcttat gcttttggtg taccacttgg tgatgaagtc
    21301 ccagatgaat ttgtcgcaaa aattgttcat ggcatgctta caggtccgtc aggaacaagt
    21361 agtgtatcaa aagcgatgta cgtgcgaaaa gacttaggaa caattattac gaatagcgaa
    21421 tttgctgcaa atgaatggtt tacattccat ggtaattatt gggtagggga ggaataaaaa
    21481 tgaaaaatat ttggaaatac ggacgaacag gtggagagta tgctggacaa gtgcttgatg
    21541 atatggttat gactgttcca tttaccgatg ttccaccact cgaaggaatt cgtgctgatg
    21601 gggaaccact aacgattgct gatcagatgt ttgatcctaa actgaaccaa tggattgttt
    21661 tagtgaatgt actagatcac aacgatttaa acaatctcaa agcgatgtat gagtcgttag
    21721 aaaatgagaa cggcgattta aaacagctca acgccaaact catgctaagc gatgtagcga
    21781 ttaaacagga aaatactgca ttgaaagaaa aagcggatgg tttagcacaa atcaattcaa
    21841 aaacgatgct tgcttcgttc caaaatagca aagacattgc agaaattaaa gagcaactaa
    21901 atccagcttc aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    21961 gattggggct gttttactga cgatcaagtt cgactattcg ttccactatg cattacagac
    22021 gaagaagcag aaaaaatcat taataaagat aagagcgcat cttaagtgat gcgtttttat
    22081 ttaaggtaaa ggagttgtca catgattaat ttaggagaat ggggaacaat cgcaggatca
    22141 atcactgcga ttgtttcttt gattttatta gtaataagac cagtcactgc atctttctcg
    22201 aagattactg aaactctttc aaaagtaagt cacaatttag atttgctgac taaagattta
    22261 gaatcgagca aatcagatcg attgatgatt catgaagaac taaagaaaca cgatgaaaga
    22321 ttagatacac atacagaaaa attggtagaa cacacacaac aaatcaaaac tttatttaga
    22381 gaaagatctc ggtaaaaaag aaaggagtta agaagaaatg attttacccg ataagtatta
    22441 tcaagtcatt aaatggacag ttttaacagt attgccagct gcatctgtat tagttgcaac
    22501 actaggcaaa gcgtatggat ggaatggaac agatatgaca gtactcacta tcaatgcagt
    22561 agcaacattt ttaggcgtta tcactggcgt gtcggcttat aatttgaaaa aataggagga
    22621 aacaaatgaa aaagaaaatt actattactg cgatgagcct attaacggct ctttttttat
    22681 tgccaattaa tgggtttgcc tatacgatta acaatgaatt taatttaggc ccaaacgaag
    22741 gtagctcaca agtagcaaat aatcagtaca ttttactgca tgaaacggct aatgaaacag
    22801 caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagcgct tatactgctt
    22861 atattgtggg agacggcgga attgtttatc aagtcggtca acctggttat gtacagtacg
    22921 gtgctggttc gtatgctaat gcaaatagtc ctgtgcagat tgagttacaa cacacacatg
    22981 ataaagcaac gtttgagaaa aactacaagg catacgttga attagctaga gattcagcaa
    23041 tgaaatatgg tattccactt acactagaca caccgtataa tcaaccaggg atcaaatcgc
    23101 atttatgggt aacacaaaat atttggggcg atcatacaga tccttacggt tatctttctg
    23161 aaatgggcgt aagtaaagaa aaattagcct atgatttggc tcatggattt accgatgaaa
    23221 atccgacaac ttcagatgat aaaccagtca ttgatccaac tcgagcaggt gctgcaaatc
    23281 ctaccctgac agatggaaca aattacgccc acattgatca gttcggagaa atcgaaaacg
    23341 caaacttaca tgtggctggt tggcacatcg ctaactataa atacgagtat atcttcatta
    23401 tggattacaa tactgggaaa gaattagctc gagtaaaagc tgatggaata tatagaccag
    23461 atgtaaatca agcttataat actttaggaa atgttggcta tcatgtatct tttaacatgc
    23521 gcaacttccc taataagaaa gtctatgtaa tgatgcgggc aacgaatgat ccagaaggga
    23581 acactaaagg cggagcacaa gattttcatg ataaacgctg gtatttaaat attccgcaac
    23641 gataaaaata gcccctcgtt
//
LOCUS       DAYYGY010000048        22987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000048 DAYYGY010000000
VERSION     DAYYGY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.049"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..566)
                     /locus_tag="ACHA52_001750"
     CDS             complement(111..566)
                     /locus_tag="ACHA52_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940447.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ACHA52_001751"
     CDS             complement(720..1928)
                     /locus_tag="ACHA52_001751"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8940448.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ACHA52_001752"
     CDS             complement(2040..2372)
                     /locus_tag="ACHA52_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8940449.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ACHA52_001753"
     CDS             2520..3395
                     /locus_tag="ACHA52_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8940450.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ACHA52_001754"
     CDS             complement(3477..4058)
                     /locus_tag="ACHA52_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8940451.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ACHA52_001755"
     CDS             complement(4064..5323)
                     /locus_tag="ACHA52_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8940452.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ACHA52_001756"
     CDS             complement(5325..6371)
                     /locus_tag="ACHA52_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940453.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="ACHA52_001757"
     CDS             complement(6606..6881)
                     /locus_tag="ACHA52_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8940454.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACHA52_001758"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACHA52_001758"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8940455.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACHA52_001759"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACHA52_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8940456.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACHA52_001760"
     CDS             complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACHA52_001760"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8940457.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="ACHA52_001761"
     CDS             complement(10709..11344)
                     /locus_tag="ACHA52_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8940458.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="ACHA52_001762"
     CDS             complement(11410..12813)
                     /locus_tag="ACHA52_001762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8940459.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="ACHA52_001763"
     CDS             complement(12800..13831)
                     /locus_tag="ACHA52_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940460.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="ACHA52_001764"
     CDS             13862..14083
                     /locus_tag="ACHA52_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8940461.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="ACHA52_001765"
     CDS             complement(14248..14985)
                     /locus_tag="ACHA52_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8940462.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="ACHA52_001766"
     CDS             complement(15138..16478)
                     /locus_tag="ACHA52_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8940463.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="ACHA52_001767"
     CDS             complement(16715..18238)
                     /locus_tag="ACHA52_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8940464.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="ACHA52_001768"
     CDS             complement(18616..19227)
                     /locus_tag="ACHA52_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8940465.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="ACHA52_001769"
     CDS             complement(19557..20273)
                     /locus_tag="ACHA52_001769"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8940466.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="ACHA52_001770"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="ACHA52_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8940467.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21602)
                     /locus_tag="ACHA52_001771"
     CDS             complement(20832..21602)
                     /locus_tag="ACHA52_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8940468.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="ACHA52_001772"
     CDS             complement(21599..21970)
                     /locus_tag="ACHA52_001772"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8940469.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="ACHA52_001773"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="ACHA52_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8940470.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6447 a         4912 c         3900 g         7728 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aattaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagg tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacact tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggttt
//
LOCUS       DAYYGY010000049        22638 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000049 DAYYGY010000000
VERSION     DAYYGY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.050"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHA52_001774"
     CDS             complement(227..1114)
                     /locus_tag="ACHA52_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940471.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHA52_001775"
     CDS             complement(1111..1980)
                     /locus_tag="ACHA52_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940472.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHA52_001776"
     CDS             complement(2117..3403)
                     /locus_tag="ACHA52_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8940473.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHA52_001777"
     CDS             3676..4254
                     /locus_tag="ACHA52_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8940474.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHA52_001778"
     CDS             4254..5183
                     /locus_tag="ACHA52_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940475.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHA52_001779"
     CDS             5180..6916
                     /locus_tag="ACHA52_001779"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8940476.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHA52_001780"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHA52_001780"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8940477.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHA52_001781"
     CDS             complement(7812..8321)
                     /locus_tag="ACHA52_001781"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8940478.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHA52_001782"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHA52_001782"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8940479.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHA52_001783"
     CDS             complement(9622..10713)
                     /locus_tag="ACHA52_001783"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8940480.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHA52_001784"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHA52_001784"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8940481.1"
                     /translation="MRYMTAGESHGAELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHA52_001785"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHA52_001785"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8940482.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHA52_001786"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHA52_001786"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8940483.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="ACHA52_001787"
     CDS             complement(14676..15650)
                     /locus_tag="ACHA52_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8940484.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="ACHA52_001788"
     CDS             complement(15631..17514)
                     /locus_tag="ACHA52_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8940485.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="ACHA52_001789"
     CDS             complement(17532..19757)
                     /locus_tag="ACHA52_001789"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8940486.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="ACHA52_001790"
     CDS             19872..20906
                     /locus_tag="ACHA52_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8940487.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="ACHA52_001791"
     CDS             complement(20973..21986)
                     /locus_tag="ACHA52_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8940488.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="ACHA52_001792"
     CDS             complement(22064..22252)
                     /locus_tag="ACHA52_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8940489.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6450 a         4864 c         4095 g         7229 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tgctccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aactaaaa
//
LOCUS       DAYYGY010000050        22175 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000050 DAYYGY010000000
VERSION     DAYYGY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.051"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..2075
                     /gene="tdc"
                     /locus_tag="ACHA52_001793"
     CDS             240..2075
                     /gene="tdc"
                     /locus_tag="ACHA52_001793"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8940490.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2380..3858
                     /locus_tag="ACHA52_001794"
     CDS             2380..3858
                     /locus_tag="ACHA52_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8940491.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3824..5155
                     /locus_tag="ACHA52_001795"
     CDS             3824..5155
                     /locus_tag="ACHA52_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8940492.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5269..7821
                     /locus_tag="ACHA52_001796"
     CDS             5269..7821
                     /locus_tag="ACHA52_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8940493.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7828..9267)
                     /locus_tag="ACHA52_001797"
     CDS             complement(7828..9267)
                     /locus_tag="ACHA52_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940494.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9242..9424
                     /locus_tag="ACHA52_001798"
     CDS             9242..9424
                     /locus_tag="ACHA52_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940495.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9613..9975
                     /locus_tag="ACHA52_001799"
     CDS             9613..9975
                     /locus_tag="ACHA52_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8940496.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10162..11019)
                     /locus_tag="ACHA52_001800"
     CDS             complement(10162..11019)
                     /locus_tag="ACHA52_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8940497.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11139..12050
                     /locus_tag="ACHA52_001801"
     CDS             11139..12050
                     /locus_tag="ACHA52_001801"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8940498.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12057..13708
                     /gene="mdcA"
                     /locus_tag="ACHA52_001802"
                     /pseudo
     CDS             12057..13708
                     /gene="mdcA"
                     /locus_tag="ACHA52_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13810..14109
                     /locus_tag="ACHA52_001803"
     CDS             13810..14109
                     /locus_tag="ACHA52_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8940499.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14099..15775
                     /gene="mdcD"
                     /locus_tag="ACHA52_001804"
     CDS             14099..15775
                     /gene="mdcD"
                     /locus_tag="ACHA52_001804"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8940500.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15779..16372
                     /locus_tag="ACHA52_001805"
     CDS             15779..16372
                     /locus_tag="ACHA52_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8940501.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16396..17472
                     /locus_tag="ACHA52_001806"
     CDS             16396..17472
                     /locus_tag="ACHA52_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8940502.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17497..18366
                     /gene="mdcB"
                     /locus_tag="ACHA52_001807"
     CDS             17497..18366
                     /gene="mdcB"
                     /locus_tag="ACHA52_001807"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8940503.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18477..19241
                     /locus_tag="ACHA52_001808"
     CDS             18477..19241
                     /locus_tag="ACHA52_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8940504.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19730..21823
                     /locus_tag="ACHA52_001809"
     CDS             19730..21823
                     /locus_tag="ACHA52_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8940505.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21848..22150
                     /locus_tag="ACHA52_001810"
     CDS             21848..22150
                     /locus_tag="ACHA52_001810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8940506.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3788 c         4725 g         6483 t
ORIGIN      
        1 tgtttgttcg ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc
       61 gcttttttgc gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc
      121 tacgtggatt tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg
      181 ttacagataa acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa
      241 tgaaagatat ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt
      301 ataaaatgtt gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc
      361 cttcagatat gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc
      421 gcgaccatat gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc
      481 catggcattc agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt
      541 tattagctta caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta
      601 tggcaacttc gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga
      661 cagacggatg gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt
      721 acgcccgttg catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga
      781 aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg
      841 aaagctttac ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa
      901 atatccaaag attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg
      961 cgttagatat ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt
     1021 atcgaatgga tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc
     1081 caattctagg tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag
     1141 ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg
     1201 ttgatgccgc atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg
     1261 tgccgtatgc ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa
     1321 cgattgacaa agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga
     1381 tagatcctca taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata
     1441 aaaatatgag aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg
     1501 ctccagatat gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg
     1561 cagtctggac agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg
     1621 gggcttcaat cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg
     1681 ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt
     1741 gggtattcaa agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa
     1801 tgttcgatcg gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc
     1861 atactacatt tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg
     1921 gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca
     1981 tgactcctta tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag
     2041 caatggaaaa gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa
     2101 caagagacat atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct
     2161 gtttttcata tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc
     2221 gttcgtttca tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac
     2281 tgggttttgt actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga
     2341 aaacaaaaac atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca
     2401 aagcaaatca cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga
     2461 aatattccag atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta
     2521 ttattgtacg cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa
     2581 aaaggtggtc cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc
     2641 tcatggctgt tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct
     2701 ccgttgtttg gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta
     2761 gttgtcattt tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca
     2821 aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc
     2881 ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt
     2941 ttttcatggg acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg
     3001 ccgatcatgt ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa
     3061 aatccagtta aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc
     3121 aatattttga atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat
     3181 aatatcgcgc aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg
     3241 aatctgttta gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca
     3301 ggaccagcaa agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat
     3361 ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt
     3421 atctcaatct ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg
     3481 ctcgtaaatt caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt
     3541 ttgagattgc gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag
     3601 aaaaagagca atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt
     3661 tctgtcggac tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc
     3721 agtgtcattt tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa
     3781 caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat
     3841 gaaacggact ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac
     3901 aggattattt ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt
     3961 ttatttgata tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt
     4021 agcaaatagt acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg
     4081 ggcatttttt gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt
     4141 aacagcaata ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg
     4201 aggtagcgca ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga
     4261 attggaatct tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg
     4321 tataggattg agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg
     4381 acagatcgat gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt
     4441 tttattcagt tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc
     4501 gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat
     4561 aggaacgatt ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag
     4621 tcctttagtg actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct
     4681 aatgattttg gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat
     4741 tttatatcaa acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa
     4801 aggcgtacct ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct
     4861 acattttttt gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag
     4921 ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa
     4981 gaaacggaac actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat
     5041 tgctttcagc ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg
     5101 gtttgtattc ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca
     5161 gatacatttt gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa
     5221 aacttccctg cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac
     5281 agattgacgg tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa
     5341 gaagaacgac agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag
     5401 acaaaacgat ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc
     5461 attgcctctt ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc
     5521 gtagtgatcg tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg
     5581 aatggtttga agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa
     5641 aaaatccctg cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc
     5701 gttccagcag atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg
     5761 acgggagaat cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc
     5821 ggcgaccaga aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt
     5881 gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg
     5941 gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt
     6001 caaggcattc tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg
     6061 gattggcata tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga
     6121 ttgccagctg ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag
     6181 gcgatcatca aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca
     6241 gataagacag gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa
     6301 gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag
     6361 aaaacagaaa atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca
     6421 gccaatctac ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat
     6481 atggcaacga tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa
     6541 gtattatttg ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct
     6601 gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg
     6661 aaacaggaac tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg
     6721 atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc
     6781 tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt
     6841 ggcttaaaac atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa
     6901 gagttgattc agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa
     6961 ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc
     7021 gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt
     7081 agtgaagtaa gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt
     7141 gcaaaagccg taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc
     7201 ttcttgccaa ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc
     7261 ccgctacctt tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta
     7321 tcttatgcac tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat
     7381 ccaaagcaag gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg
     7441 atcatggttc ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc
     7501 attcttctgc aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg
     7561 tccaagccgt cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt
     7621 atccttgctt tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt
     7681 acagtaagtt taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc
     7741 ttagttgtag aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca
     7801 gctagagaat acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa
     7861 acgattgagc tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga
     7921 atctagaggg aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc
     7981 ggttagttcg ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg
     8041 caagaacgcc ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg
     8101 tttgatagaa aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg
     8161 ttcattgatt tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa
     8221 gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc
     8281 aatcaatatc ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga
     8341 tgtttttttt agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag
     8401 gacctctaaa aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt
     8461 gtaattccaa ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata
     8521 aataccttcc gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct
     8581 gttggtatgg atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa
     8641 gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt
     8701 tcttaaaaat tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt
     8761 atgagtgaaa ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag
     8821 aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt
     8881 gattttagtc acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc
     8941 agggacagca actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg
     9001 tttgaaaaaa atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat
     9061 tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag
     9121 gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat
     9181 ctttgtatga ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt
     9241 aatgattttt ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga
     9301 gaaaataaac ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc
     9361 atttactgat gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa
     9421 ataggtgggc aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc
     9481 ggattgaaca tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta
     9541 cttgtattca tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag
     9601 agatggaagc agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat
     9661 ttgtctccag aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa
     9721 agcaaaaaat ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca
     9781 agtgaccata ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt
     9841 ttgcgaggaa aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct
     9901 attgatctac gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt
     9961 aaatcaacgc aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct
    10021 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt
    10081 attttctaag aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat
    10141 tttgtcccag ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat
    10201 cattttgatc aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa
    10261 gggcaaatct ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac
    10321 ttttgggaaa aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc
    10381 atctgtcgtc gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt
    10441 atgacggaag gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa
    10501 ttgcagatgt tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc
    10561 tttcatgata ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg
    10621 atcggtaata tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt
    10681 ttccataaaa attcggacca agttcggaat agaaacagag gctacctcta aatacaatat
    10741 gacggaagta acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt
    10801 gtctaatgtc ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt
    10861 tctgccgcta tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg
    10921 gctcaaggca ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc
    10981 gaatactttt ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac
    11041 ctttttgtta tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga
    11101 tagcagtgga aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc
    11161 ctggacaagg cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac
    11221 gtttagaaaa tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg
    11281 tttttatcca gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa
    11341 acggtattca gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg
    11401 cttgtcgatc tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa
    11461 tgaaagaagc ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg
    11521 aagctgagga aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg
    11581 aaaattgtcc gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg
    11641 cagaagccaa acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc
    11701 actgtctctt gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc
    11761 tatctgatgc tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag
    11821 aagagattcg taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga
    11881 tggacttggc aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt
    11941 tgacaaagct gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag
    12001 accaacatgg tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg
    12061 aaaaaccaaa ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa
    12121 tcagcaactt gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa
    12181 aactgattgc tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt
    12241 ttctttcaga aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca
    12301 tgtctagtat ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga
    12361 tcgatttctc ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca
    12421 caatgaaatt aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt
    12481 tgattccaaa tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca
    12541 caggtttcaa tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta
    12601 tcgtgatcgt tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg
    12661 actgggtaga cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac
    12721 gagatcctca aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa
    12781 tctacgcaga acacggcgta caatctttga atcacggtat tggattcaat acagcagcta
    12841 tcgaattgtt actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga
    12901 actgggtatt gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa
    12961 gcatccatag ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg
    13021 tcttttttgt cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag
    13081 gacaatatgc catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact
    13141 cttctaccgt aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata
    13201 atcctggcgg tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc
    13261 cattagggaa aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa
    13321 aaccagtctt tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca
    13381 atgcaatgca ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg
    13441 aattgcttat ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat
    13501 tggcggtgtt acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc
    13561 agccggcatc gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc
    13621 attattagct gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc
    13681 accaatgaaa ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta
    13741 cccagatgga agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg
    13801 gagagatcca tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa
    13861 atccatgtag gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa
    13921 acagaatcta ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat
    13981 gtattaacga gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc
    14041 ggtgctacac caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat
    14101 gaaaaatagt tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg
    14161 acgtgaattg attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat
    14221 cgtccctgaa agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc
    14281 agtagtaatc gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc
    14341 aaaaatcgtt gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt
    14401 tccagtcatt gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt
    14461 atctatttca gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg
    14521 tttgattcca ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag
    14581 ttatttaatc gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca
    14641 agaagcaggc gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg
    14701 atctcgtcaa cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc
    14761 aatcaaagct gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag
    14821 ctcagcattt tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga
    14881 cgattacaat gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc
    14941 agaaggaaat gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg
    15001 catgaaaaat ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat
    15061 agaagaagcc atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg
    15121 aaacggtgaa gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta
    15181 cgaagaagat aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag
    15241 ccaagcatac ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc
    15301 tgctgcttat gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa
    15361 tgctatttca ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa
    15421 caataaagaa attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg
    15481 ttcggttgct gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa
    15541 taattataca aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc
    15601 ttcagaacaa aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg
    15661 caatacaaat gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg
    15721 acgtgtcgaa acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat
    15781 gattcaagca catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc
    15841 tgaatgggtc gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca
    15901 cgcagaaata ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga
    15961 aatctcaaaa catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc
    16021 gtatagacaa gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa
    16081 catattatca ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg
    16141 gattccggca gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat
    16201 tgaattgcca attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca
    16261 agtagtgact cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa
    16321 aattctattg aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa
    16381 aagagagaga aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag
    16441 aaagtatcag ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac
    16501 agccacagcg caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca
    16561 gcaccaatgt ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct
    16621 tattgggaat ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg
    16681 acggcttatc tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca
    16741 agctttttac tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg
    16801 atgctgcaga tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct
    16861 aataaaaaat atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat
    16921 ctgttctatc aaacacaagt attgactggg ccttatcatg acagctggta cttattagat
    16981 cggtttgttg ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc
    17041 tatcaatatt tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc
    17101 ttttctgtcc gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac
    17161 ttgaatacga tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct
    17221 tccaaaatga ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat
    17281 gcttatcgta cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa
    17341 gatagttggc tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca
    17401 agttgtgcat ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt
    17461 ttcagcaagt aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa
    17521 gcagtgactc agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta
    17581 agtccaaaac ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat
    17641 gctttatttc ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata
    17701 gcatggcatc atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa
    17761 gcggaaaagg caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg
    17821 gcaatggggc tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct
    17881 ttatcgaaaa tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt
    17941 cataaaaaaa ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat
    18001 gaagaagcga tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta
    18061 gataatgatt catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta
    18121 gatgacacat gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt
    18181 gctcatgctg ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt
    18241 tgtcaggaaa accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc
    18301 atgctgaaga tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat
    18361 gactaaataa gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct
    18421 tttagcaagg ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga
    18481 aaaaaatcaa ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga
    18541 cccaaggatt cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg
    18601 gcagaggttc atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg
    18661 gcagagattc ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg
    18721 gatttcttgc attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga
    18781 ataaaagaca aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta
    18841 aagaaaagaa agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt
    18901 gggaagaaga aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac
    18961 atcagaaagt tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa
    19021 ctattcagga agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg
    19081 atttcagatg ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta
    19141 agacacaacg acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa
    19201 gatggcaagc cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg
    19261 gagttgtgac aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata
    19321 gcctatcggc aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt
    19381 cttatcgctc aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc
    19441 ttgcatctgc gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt
    19501 tgttcttatt acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca
    19561 aaatttttgt aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct
    19621 tattttttgt ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt
    19681 tattatgtaa gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag
    19741 aacaattgaa atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct
    19801 agcaagtgag ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat
    19861 ctggttgagc agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt
    19921 gaagctggaa aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg
    19981 ctttttgaat cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat
    20041 cccgatccat ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga
    20101 agatatgaga cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg
    20161 aaagcaagga ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt
    20221 tatcaatcag aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc
    20281 ggctcaaaaa atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat
    20341 ctattccaat aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt
    20401 tacattgatc tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa
    20461 aaatacagaa ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt
    20521 tcaattggac aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt
    20581 caatgctaga gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca
    20641 attgatccaa tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt
    20701 attcgaaaat ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca
    20761 agtcatgaat ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt
    20821 cactcatttt gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat
    20881 agcttttctt gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta
    20941 tgtttataaa tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga
    21001 gaatctgaaa gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat
    21061 cgaattagta gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg
    21121 aaataagccg cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga
    21181 agactttttg aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac
    21241 ggagttattc actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat
    21301 catctatggc aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga
    21361 gatccagcct atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc
    21421 cactacttgg gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt
    21481 cgaagagcgc tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt
    21541 gattggcaaa cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg
    21601 agtcagcgta gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt
    21661 aaagattatt ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga
    21721 actaattgaa ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat
    21781 cgaagatttc aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag
    21841 aacaaagatg aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt
    21901 tgcagcacag atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga
    21961 ccattgtgat atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa
    22021 ttttcgttcg cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa
    22081 aaatattgtt tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg
    22141 agttatctaa atacaaaaag aaaagagggt aaaaa
//
LOCUS       DAYYGY010000051        22066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000051 DAYYGY010000000
VERSION     DAYYGY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.052"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            361..567
                     /locus_tag="ACHA52_001811"
     CDS             361..567
                     /locus_tag="ACHA52_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940507.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            763..930
                     /locus_tag="ACHA52_001812"
     CDS             763..930
                     /locus_tag="ACHA52_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8940508.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            956..1300
                     /locus_tag="ACHA52_001813"
     CDS             956..1300
                     /locus_tag="ACHA52_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8940509.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1305..1586
                     /locus_tag="ACHA52_001814"
     CDS             1305..1586
                     /locus_tag="ACHA52_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940510.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1689..2003
                     /locus_tag="ACHA52_001815"
     CDS             1689..2003
                     /locus_tag="ACHA52_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8940511.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKINIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1981..3675
                     /locus_tag="ACHA52_001816"
     CDS             1981..3675
                     /locus_tag="ACHA52_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8940512.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3695..4873
                     /locus_tag="ACHA52_001817"
     CDS             3695..4873
                     /locus_tag="ACHA52_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8940513.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4836..5522
                     /locus_tag="ACHA52_001818"
     CDS             4836..5522
                     /locus_tag="ACHA52_001818"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8940514.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5522..6682
                     /locus_tag="ACHA52_001819"
     CDS             5522..6682
                     /locus_tag="ACHA52_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8940515.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6692..7567
                     /locus_tag="ACHA52_001820"
     CDS             6692..7567
                     /locus_tag="ACHA52_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940516.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7564..7875
                     /locus_tag="ACHA52_001821"
     CDS             7564..7875
                     /locus_tag="ACHA52_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940517.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7865..8218
                     /locus_tag="ACHA52_001822"
     CDS             7865..8218
                     /locus_tag="ACHA52_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8940518.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8208..8609
                     /locus_tag="ACHA52_001823"
     CDS             8208..8609
                     /locus_tag="ACHA52_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940519.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8602..9006
                     /locus_tag="ACHA52_001824"
     CDS             8602..9006
                     /locus_tag="ACHA52_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940520.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9018..9626
                     /locus_tag="ACHA52_001825"
     CDS             9018..9626
                     /locus_tag="ACHA52_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8940521.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9646..10008
                     /locus_tag="ACHA52_001826"
     CDS             9646..10008
                     /locus_tag="ACHA52_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940522.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10011..10193
                     /locus_tag="ACHA52_001827"
     CDS             10011..10193
                     /locus_tag="ACHA52_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940523.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10210..13641
                     /locus_tag="ACHA52_001828"
     CDS             10210..13641
                     /locus_tag="ACHA52_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8940524.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            13692..14429
                     /locus_tag="ACHA52_001829"
     CDS             13692..14429
                     /locus_tag="ACHA52_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8940525.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14439..16730
                     /locus_tag="ACHA52_001830"
     CDS             14439..16730
                     /locus_tag="ACHA52_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8940526.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16754..18880
                     /locus_tag="ACHA52_001831"
     CDS             16754..18880
                     /locus_tag="ACHA52_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940527.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            18897..19046
                     /locus_tag="ACHA52_001832"
     CDS             18897..19046
                     /locus_tag="ACHA52_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940528.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            19043..19489
                     /locus_tag="ACHA52_001833"
     CDS             19043..19489
                     /locus_tag="ACHA52_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940529.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19491..19628
                     /locus_tag="ACHA52_001834"
     CDS             19491..19628
                     /locus_tag="ACHA52_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8940530.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19666..19959
                     /locus_tag="ACHA52_001835"
     CDS             19666..19959
                     /locus_tag="ACHA52_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940531.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            19956..20180
                     /locus_tag="ACHA52_001836"
     CDS             19956..20180
                     /locus_tag="ACHA52_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8940532.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            20177..21202
                     /locus_tag="ACHA52_001837"
     CDS             20177..21202
                     /locus_tag="ACHA52_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8940533.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            21392..21465
                     /locus_tag="ACHA52_001838"
     tRNA            21392..21465
                     /locus_tag="ACHA52_001838"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21426..21428,aa:Ile,seq:cat)
BASE COUNT         7747 a         3352 c         4610 g         6357 t
ORIGIN      
        1 tcgtaataac ttttgagtaa atagaacaaa aaacctgcac tagtttccgc tagtacaggc
       61 agtgactata tcggttgata atctagcata tattaaaaat aattgcaaga aaagaaattt
      121 atagtggttt ataccaaatt atcaaagcaa tgttattttg ttgctgtcta ttgtttttta
      181 attattcaca cgataactaa aggtgggtga agagaaatga ttccattaat aatttcaatt
      241 tttgcgctct gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa taaaaaatag
      301 tagcagccaa gaataatttt atagtgtcac tgtggcggaa agggtagacg ctaagcatgt
      361 gtgctaggtc aatgcttcgg caaccatgca aggttcgatt ccttgccagc gacattaaat
      421 gcctatgacg gttacgacta ccgaaaaaag atcgttaaga agctatacgg tgctacgtac
      481 ggcaatgtag taagtgtgct atctgtacac caccaagctt cggtcactgt ggcggaagta
      541 gaagacgcag cggtaaatgg cgagtagcct cgtgagagcc tggtaagttc tcgtgagtgg
      601 tgcaatccca ctccagcgac tttaagcaac cgaggcatcg gcggtttaaa aatatagggg
      661 tgcgcaattt cgtacgcgtt ttgtgcatcg tgcaagttac tattacatat tagatcactc
      721 tttgagtggt ctttttattt ttgcacaaag gaggaaacaa caatgtatag accacaatac
      781 ttagaacaga agtatgaagt aatcactgtt cataaaggta atggcgaaaa agtatatgag
      841 tatagaagac caataaagag cgatacatat aaacgaaagg aaaacaatga agttattcca
      901 ttgtatggca aaagaatagc taagcattaa ataagattgc gaaaggagac ggaacatgac
      961 cgaggaattc tatagatggc tattacagtt gacaagagaa gatcgtttgg ttaagttcta
     1021 tcagtctcct aaatggcgca ggcttagaga gaaagcgatg aaacgagatc actatgaatg
     1081 ccaagagtgt agaagactag gtaagtatca tagagtagag aacgttcatc atataaagga
     1141 agtcaaggat agacctgact tagctttaga tttagataat cttatttgtt tatgtgttga
     1201 acatcataat gaagttcatg gcagatatct tacagcatta gataaacaag agaagaagat
     1261 agaaagcttt gctaacttcg atgcaagtga aaggtggtaa gtgcatgatc atcaatgata
     1321 atggcagaga gtatgataca gaaaagattg aagagtattc atcttatact cagggattaa
     1381 ttaaacgttt gatatacgtt cgctatgtag gtattaggga tctgttatca gataactgtt
     1441 gtagtaaata caaagtgaat caagtaagag aagcgttgaa taaagataat aacgtcgaaa
     1501 gaataaaaaa tgtttttgga tatagtattg aagagattaa ttattacatt gacttcgctg
     1561 aagctttcat tccgatggtg agataacccc cccttaaaat aaatcgcaaa ttttttgggg
     1621 gtgatgaaac ggagggggct gtcaggaaaa gagatttttt cgaactttat catgaaagga
     1681 gggctaaaat gtttaaaaac gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct
     1741 ctttaataag ccaattggaa agtcagaaaa taaatattga gccattctta gataatgttg
     1801 atcgttatat cagtttatgg gaaacggcga tatcactgga agaagatata tccgagaacg
     1861 gcattagact ggagaatggt aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca
     1921 aacaaatggg attgatgttg gataaacttg ccattactcc tgaattggta ggtgaagcaa
     1981 atgaatcaat tcctgagtta taagcatatt gaaaattggt tcaaagctat agaagaaggc
     2041 actatcaagg tatgcaaaga gcaattattg ctaaaaaatt atctagaaga aagagtcttt
     2101 actagagaag atatttactt cgataagcag atggtagagg attcaatcaa tataccagca
     2161 caatactttc cattcgaatt aattccgtgg gaaaaatttc tacaatgttt tatttatggt
     2221 gttcgatgga aaaaagataa aacactagtg ttcaatagat atctttcatt aatgggacgt
     2281 ggtaatggta aaactggttt tgcttcttgg aacaacttct ttctactaac cgctaaacac
     2341 ggtattaaaa attatgatat tgatatctat gccaataatg aaagccaagc aaagactagt
     2401 tttgatgatg tatttaaagt aattaaagat catcctgatt tagataaaaa agtatttaaa
     2461 gctacgaagg aagttattca aaatatcgct acaaacagca aacttcgtta taacacggca
     2521 aatgctagaa caaaagatgg gaagcgacca ggtgcaaacc gctttgatga aattcacgaa
     2581 aatgaagatt attcaatgat aaatgtggct acttctggtg gtggtaaaat tcgagattat
     2641 agagaatttt atgatacaac taatggtcat gttcgtggtg gtccgcttga tgacattata
     2701 gaagaatcaa aaatgattct ttctggagaa cttggaattg acaaggatgg agcagaattt
     2761 tctagtttgt ttccatttat ttgtcgcttg gataacgata atgaagttga tgatcccgac
     2821 atgtgggaaa aagcttgtcc aactattaat tacaatgcag atttaaaacg gaaaatgttt
     2881 caagaatact ctcaaatgca acgtaatgct ggtttaagac ttacgttcat gaccaaacga
     2941 atgaacagac ctatggaaga tacacgattt gctgttgctt catatgatga tgttctgcat
     3001 acgaaagaaa aagaatttcc tgaaaaaatg gatgaagtga taggaacagt cgattttgct
     3061 gatagacgag attttgccag cgttgggttg ctaggaaaat acgataaaga tgtttatttt
     3121 acacaacata cttttatcca cgaatcagcc cttcgattac aaaacatcaa acgagaggtt
     3181 atagatattt ctatagatca aggaaaatca cagatcgttc atggaaaaaa tatagaagct
     3241 gattatattg taggttggtt tcttgaaatg agtaataaat attatattaa aaaaatcgct
     3301 atggatatgt accgtgcaaa aatattgaag cccgctttag aagaagcagg ttttactgtg
     3361 gaaattgttc gaagcggatc tgttacacat ggtatgttaa aagatctggt tgatgacctt
     3421 tttattaatc aacgtttatt ttttggtgac gatgcgatta tgcgttggta ttgcatgaat
     3481 gtatatgaag agcatatttc taatggaaat atacgctatg aaaaaataga acctgaaact
     3541 agaaaaacgg atggcttttt ttcattcctt catggtttga attttttaga tgatatttat
     3601 gattctgctc ctgtaacagt cacaaatagc tcagtagaaa atacaggaac tggatttact
     3661 cctctagtat tctaacttga aaggaggtga gaaagtgggg atttttcaaa aggcggtagg
     3721 atacttcaca aaaaaagcaa cggttccttt agaagaatac ttttgtaaat tgcaagttga
     3781 ttttgtgtat cgaaaatttg cgattgaaac ttgtattgat ttgattgcaa atgcgatgag
     3841 taaagcggaa ttcaagtcat atgaagatgg aaaaaataaa aagaatgatc tttactatag
     3901 gctgaatgta gctcctaata agaaaaataa tgcaacagaa tttagaaaaa aactgatcag
     3961 gagattaata ttctacaatg aagtattgat cgtttctccg tctaataatt ctagcgaaat
     4021 atttattgcg gatagttggg atgtcacaga atatgcattg aaagatgatg tgttttctca
     4081 agtgcaaatt aacaacatag tccttgatag agaatttcta gaaagtgatg ttatctatat
     4141 aaaatacgca gatcaacaaa ttaggcaact agtcgatgcg tattatcaag cgtatgggaa
     4201 actcatttct agtgccatga atgtttacaa gcgctctaac gctcgtagat acgtactgaa
     4261 agggaattta ttccgaccgc aagacaatac aacacaagat caaatcaata aaatgatgac
     4321 atcacaattt aaggctttta tggaagctga taatgcaggt gcggtatttc aattacaaaa
     4381 tgagtacaca ttagaagatt tcagcggaaa cttccaaagc aattcaagag atataaaaaa
     4441 cttaatagac gacatctttg agatgacagc agcagcgttt cacgttccga aaaacctact
     4501 aaagggagac atgagtgggt tatcggatca agtggacgct tttttaatgt tcgaaatcat
     4561 accgattgct gaacttattc aggatgcgtt taacgctagt ctctatgaag tagaagaata
     4621 cttgtcaggg aattttgtac gtgtggatac aactatgatc aagattacta gcttcaaaga
     4681 tttggttgac gctattgatg taggcattag aaatggagta tttacaatca acgaaggaag
     4741 agaacgcgtt ggaaatgatc gctctgataa ggcgatggca gatgaaatat ttataactaa
     4801 aaacaatcaa caagtatcga aaggaggtga ggcgaatgac gacaatgaaa acatttctag
     4861 cagtaaagaa tgaaggcgca gtaccgcaaa tttttattca gggatttatt ggttctagtt
     4921 ggttctttga agggaatact gacaagggaa tcaaaaatat tttggatagt ctaggtgatc
     4981 aagaagaaat tgaagtagta attaattcaa acggtggaga cgtatttcaa gggattgcta
     5041 ttgggaactt acttaagtca aataaagcaa aagttaacgt tgtgattaac ggcttagccg
     5101 ctagtgctgc ttcaattatc gcaatggctg gcgatactat aaaaatctac aacaatgcac
     5161 aattgatgat tcaccgcgct tccacatacg gagaaggaaa tgtcgatgac ttccgtacga
     5221 ttgctgacca actggaatca attgataaat cggtaaaggc ttcatataaa acacgattca
     5281 atggcacaga tgaagcattg caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa
     5341 cagctttgag ttatggattg gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg
     5401 aagctaaaaa agaacaaagc gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag
     5461 aaaaaattgc tgcatttaca gcagcattaa ataaaacatt tggacaagga gatgtaaaat
     5521 aatgacagtt aaaaatttaa aaggtgtaac agctgcaagc gaccaattga tgaaagcttt
     5581 taaagatggt aacgaagaat cttttagcgc agctatggta agcttatcta aggaaattca
     5641 ggataaaatt ttagaagaag caacagcaaa aaatcaagat caattagtat taatgaaccg
     5701 tggtcagcgt gtattaacta cacaagaaac aaaattctat aacgaagtag tgaaaaacga
     5761 aggttttgca ggggtcgaag aattagtgcc agctactgta tttgaacgtg tatttgaaga
     5821 tttagaacaa tctcatccac tattgcaaaa aattactttt gttaacacaa ctggtgtaac
     5881 agaatggatt gtgtcacgtg gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt
     5941 taaaaaagtt ttagataatg gctttgatgt aattaacatg aagcagttca agctatcagg
     6001 ttatattcct gtatgtaagg caatgcttga tttaggtcca gtatggttag atcgttatgt
     6061 ccgtactgtt ttagtagaat cattgagaat tgcattagaa caagcaattg ttgatggtac
     6121 tggtaaagat atgccagtcg gaatgatgcg tgacatgagc aaacaaacta gcggagaata
     6181 tgctgaaaaa aaagcagaac ctattacagc tttagatgct gtaactatgg gcggtttgat
     6241 ggcgcgacta tcaaaattca atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa
     6301 tccttctgat gtggtcctaa ttgtgaatcc aactgattac tggtctaaag tattcccagc
     6361 taagactgta ctaactgcta atggagaata cgtacaagta ttgccagtac cagtttcaga
     6421 tttgcagtca acggctgtgc cagaaggaaa agcagttatt ggggtagcct cagattactt
     6481 catgggtgta ggatctacac taaaaattga agcttcagat gaataccatt ttgttgaaga
     6541 cgaacgcatt tatctagcta aacaatatgc aaacggtcaa cctaaacgta acgatagttt
     6601 cattgtatta gatattagcg ctttgggaac tactactaca actacaaaac caacaaccac
     6661 aacaactaca acacaagcgt aggtgatcag aatgaagtat attctttgtc agccggcaat
     6721 caatcggttt aaatgggagc ttgaagtttg tttaactaat ctgaagaaac taggaatcaa
     6781 agatatcgta ttgcttttca gcagacacga tgatcagatt cctatttttt ttgagaagga
     6841 atatggtgtt gaagttcatg tgtacgatga tctgcgggac gacaaagagt atattccttc
     6901 gattaaacca tatttatggt ggaaatattt agaagaagat cattcgcgtg aggacgaccg
     6961 atatttctat atcgattcgg atgtcatttt caataaaaga attaatttgc gcaaattgcc
     7021 ttctaaagat gatgtttggt attgtagcga ctgctgtagt tatctaagtc ttgattatat
     7081 tagaagctgt gaaaacggag aaaatattct aaaagatatg gcaaacattg taaatgttac
     7141 agtagaatct ttggaaacta taaacactaa ttcaggaggc gcacagtggg ttattaaccg
     7201 tcctaaagcg aattattgga aaaaggttta tctggattct aatcggctat atcgctacct
     7261 tagagggcaa aaaacaaata tacaaatctg gacagccgag atgtgggcac agctttggaa
     7321 catgatgtat ttcaatattg gtcctaaagt tcacgaggaa ttagactttt gttttgctac
     7381 tgatccaata gaaaaagtta aagaagtaaa aatcttacac aatgctggag taacaacaaa
     7441 cgatgaagat ttatttttca aagggagata cgtgacttct acgccttttg atgaagattt
     7501 atcatttgta aacaagaaaa aatgctctta cgcatatgtt aaagcaatta aggcggtggt
     7561 tagatgacgc ctgaacaagt gactgaagaa ttgctaatag ctgtgaagga taatatttac
     7621 gttacctgga acgaagaaga tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat
     7681 cttcaaagta aagtgagtac aacactttct ttttctcctg aaagcttaga atacggattg
     7741 ctaatcgaaa gatgtagata cgactggaat cgtgctttag atgagtttga acaaaatttc
     7801 gctagtgagt tattaggttt cattcaacat tatgcgctac aagaatatat tgcaggtgat
     7861 gtgaatggcg aataatcgta gactcgaaga aacattcaac gatggttggt taaagatttt
     7921 gacgcaaact accaaaagaa atgaactagg aaaaaagatt ggtgtagaag atacagaaat
     7981 cacttcttta aaatttagaa atctttccat gagagatagt gatataacag ctatggatgc
     8041 gatgggatcg aaattaacta agaaagtaaa gactccattt catccaatcg ccaagaaatt
     8101 taataaagat caatatttta tcgtaatcga tagtatgcgt tacaacgtta tctatgccga
     8161 ttacgataat ttttatatct atttttatct tgaaagtgtg ggtgaatatg gtgattgata
     8221 attctaaaga aaaagaacgt ttaaataagc aaatttctgc tatcaaaact tccttagaag
     8281 agcattttgg cctcaaactc tttcaagact ccgtaggcga ggatgagcta cctgatgatt
     8341 ttaattactt cattctcgaa acaggagaaa tagaaatgat cactgagcca aaatatagcg
     8401 tgggtcaaaa tctatatcta actttctatt cagaaaatag agaagattta acaggagact
     8461 cactagatat tatttcattg attcaaaatc gttcgattcg ttttcagaga atggatccca
     8521 atcatttaaa actagagaac caagatcgct atatcgatca attggtattt acgtttagac
     8581 gattattgaa gagtgattgt catggctaaa aatagttggg agctaaaaat aaatggacat
     8641 gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga gcgaacgact gattaacgaa
     8701 gcattgaaat caaaaggttc ggctattgca gtggatagga ttacagaaaa aattcctgtt
     8761 tctgaagcag atttaagaag agggcaccaa cacgcaaaaa atagtcgtcc acttaagact
     8821 caatacatta atttgggttt catcattaga cctacaagaa aatttgagta tttaaaatat
     8881 cctgatttgg ggataggtac ttctaaaaga aatcagccgg acgaatttat gagaagagga
     8941 ttaggtcttg cacttgatcc aattacagaa cttctgattc gtcaattcga taaattaaat
     9001 aaataggggg aacaacaatg gctaaaacaa aaactgtagt aacaacgttc gataacgtaa
     9061 gtatcaaacg aattgctttt aattttaaga acgcaggaaa tgcaatcgca acgaattgta
     9121 atggacaatt agatggcgaa acagaaatgc aaacggtggt taaaaaatgt ggagcgacag
     9181 aagtaaaatc aaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga
     9241 tggaagttta tcgacgtttc aatgggttga aacaagatgg acgtattaaa ccaggcattt
     9301 actcttacgg tcctgattcc gtaggcgaag atttctcact tgctgcagag atcgtggatg
     9361 acttcgaaga aaatagaaag ttagttggta tgttagcatg cacttcgaat acaggattaa
     9421 cattctctat tgaaaatggt gcggatgaag tagctgcgtt agaactagaa acaaaagtta
     9481 tgcaagatga atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact
     9541 taacagatca atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga
     9601 caactacggc cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac
     9661 tcaaaaattg aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag
     9721 aaattattga tgatcaatag agattttaac acagacgagt ttgcaaaaat gactgtggga
     9781 aaaggatcca tggatatttc tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc
     9841 caagcgaaca tgactgatta tatttcattc gatgaattta tcgataaatg ggattttgat
     9901 atggctactg ccagctatat ttatcaattg atgatgttca aacaagcacg cgatgcttat
     9961 caaaaagaat ttgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa attccaaagc
    10021 tcttagttga aacgtgggtc gatgtctatt cgatgttgac cgacgttttt tctatgcctt
    10081 cagatttggt tttaagcgat atctgtttag atgacatttt gcaaatggct tacaacaaga
    10141 gtgcttatga aggatggaaa aactatgcaa taaaccaatc ccagaaaaac taaagaaagg
    10201 aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa ctttcatagc taacgatgac
    10261 ggattgaaat ctacgttaaa agaaatcagt gctgaattaa ctaaaaatag agcagaatta
    10321 aaactagaac aagctcaatt acaacagact ggttctgaat cagacaagtt aggaagtaaa
    10381 ttatcttctt tagaaaagca gtatgaatta caaagtcaaa aagttgaagt aactagccaa
    10441 cgtttagcca atgcgaaaaa atattatgga gaaaattcca ccgaagttca gaaacttgaa
    10501 agagaactga ttaatcaaca aacagcgcaa caacgtttgt caaacgaaat tgataaaacg
    10561 agtaatgcac tagctcaagc aaaaggcgaa atacagacgt acgagtctac aatgcaacag
    10621 ttggatagcg aacaaaaaaa tgttcaagct agtgcttctc tgattgaatc cgaatacaaa
    10681 aaatggcaag caactgctgg tcaatcagct tctgaagccg agaaattagc gaaagcccaa
    10741 gaatatgttt ctcaacaatc tgaaaatgcg gagaaaacga tagatatcct aagacgacag
    10801 ttagaagcta cacagtctga atttggcgct acatccacag aagcaatgca gatggaagcg
    10861 aagcttaatg atgctgaacg tgaatttgaa gagttaggac aagctgctaa aaatgtagat
    10921 acaactaact tggacgatat cggaagcaaa atagatatga ataatttaat ggaagcttct
    10981 gacgttttaa gcgacattgg cgataagctt acagaattag ggaaacaagc agtggactct
    11041 gctaacagtg taggtagttc ccagagtaag atacaagcta attttggttt gtctaaacaa
    11101 gaggctgaag aattaacgaa tgtagccaga gacatttatt ataaaggttt tggagaatcg
    11161 ttagatcagt ccacagatgc attgattttg gtaaagcgta atttaggaga tttaaataat
    11221 caagatttac aaaatatcac ggaacaagct atggtcctag aaaacaccat gggcgctgat
    11281 atggatgaaa cgttacgtgg tgtaaatggc ttaatggtca atttcggctt gagtgctcaa
    11341 gatgcaatgg atttaatggt ttcgggtact caaaacggtt tagataaaac gcacgaatta
    11401 ggcgacaata tggcagaata tagccaatta tggagtcaaa tgggatattc agctgatgaa
    11461 acgttcggaa tgcttcaaaa tggtttagat gcgggtgctt ataaccttga taaagtcaat
    11521 gacttagtta aggaaatggg aatatcgtta acagatggtc gatttgagca aaacatggat
    11581 atgtttagtg aaagtactag aaaagctttt gaagagtgga aaaatggcgg aggaacacaa
    11641 aaagacgtta ttaattccat gattcaagat tttagcaata tggatggtca atacgaccaa
    11701 ttaaataaag cttcgacaat ttggtctgcg cttggcgaag ataatgcgat gaaagttgtc
    11761 caatctttaa ctgatgttaa ccatacattt gatgatgtta gtggatctgc acaaaaaatg
    11821 aatgaagatt ctactactcc gttgcaagag ttgaacggga aaatagctga attaaaggat
    11881 tcattagctc ctataggcaa cacaatcata gatgcactcg aaccagtaat tgattttcta
    11941 ggaaagatgg ctgatgcgtt taataatctt ccacaaccag tacaggattt cattgttgct
    12001 ataggtggtc ttacagcagc atttggcata ctgttaccgg caatactagc cgtgtcatat
    12061 ttatttggtc cgatgatgct tataattgga ggaattatag ctgttatagc aggcgttatt
    12121 gtggcaatta agaactgggg tgcaattact gactggttta gtggtttatg gaaaaaattt
    12181 actgattggt tgggtgatac ttgggaaagt ataaaagacg gagcctcatc agtttgggat
    12241 ggggttaaag aaacctggtc tggatttgta gattgggttc aagatatttg gcaaggagtt
    12301 tctgattggt ttggagagct atggagcgga ttagttgaag gagcttccaa catctggcaa
    12361 ggagtccaag aaacttggca aacattcgtt gattgggttt caaatatttg gaacggagtc
    12421 aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat accatggaca
    12481 ttaataacgt cattgattca agctggtatt aatattatcg tgggtatttt tgatgtagct
    12541 ggacagttat tgggcgcagc ttggcaagct gtttggacac ctatttctga tttccttaaa
    12601 aatacttggg atactatgac acaatggata agtatcgctt ggaatgggat tgtaactaca
    12661 ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac agctattagt
    12721 actacggctt caaatatttg gaattcaatt agtgcaacag cttctagtat ttggaacagt
    12781 atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca aaatatttgg
    12841 tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac agcatcaaat
    12901 atctggaata gtgtgactag tagtataagc aatgctataa acgcagctaa aagtgccatt
    12961 caaagtgttt ggaatagtat atcttcgtgg attagcggaa tttggaacgg tatcaaaaat
    13021 actgctttga atctttggaa tggaattaca agcactatta gctctaaagt aaacgatgga
    13081 aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta ttgtttccga catattcaat
    13141 aatgttaaaa gtacaattgc taacatatgg gaaggcatca aaaagactgt tagcgctcca
    13201 attgattgga ttagagacaa aatcagtggc atttttgata atttgaatat ttctatacca
    13261 catattccgt taccggaatt tatcatggaa ggcagcttta acccgctaaa aggtcaaatc
    13321 ccccatttgc gtgtaaaatg gcatgctaaa ggaggtatct ttactaaacc aactttacta
    13381 ggtggaatga acggtgtcgg tgaagcagga cctgaagcag ttttaccttt gaaaagatct
    13441 gttttgcaag aaattggtga tcgtatcttg agtagcacat cagtttcatc tagggcacaa
    13501 acgattcaac ctgtgaataa ctacgaattc aatttcacaa ttgatggtaa cgcagatgag
    13561 gttactatga agcaaacaac tcaacaaatc attgatagca ttacaaaaat tcaaaatgat
    13621 aatgcttcgg catggcgtta aacaggagag tatttctcct gtttttttag tattaaaaag
    13681 gatgtgaaaa aatgactgat tgtatacatt ctataatcga tggatttcct gattatttgc
    13741 ataaattggc tttagctgaa agaccaacca taccttctcc aaaaagacag agagttgaaa
    13801 cttctgtttt aggtaggtta ggtggcttag tacaagatta ctcgtttgaa gatatgtcgt
    13861 ttacattgca ctataactat ttagaggatg tggaagacca tcaagcgttc aagcaatcgt
    13921 tttatatcat gcgtcattgg ttaaactatg caaagaaatt agaattctct gacgatccca
    13981 acgtctatta cgttatccag actatcgata ttggggatgc agaaaacgat attgttgaat
    14041 ggggagagtt cgatgtaaat attactgcga aaccattcgc aagagttcaa gaagatgtac
    14101 ctataaccgt agataaacca cagtcattta acttgctgaa taatagttta gaagaaagtt
    14161 ttccaaagat tatcatcact ccttcagcta cttcatgcca gttcatctta aatgattatg
    14221 tgtttagttt cgaaggctta gtagcaggaa ctgatgtagt cattgatagt gatttgatgc
    14281 tttgctacga agagcaatcg gacggagata ttttagatcg gtccaacaaa atgaagacca
    14341 tgcaatatcc gacattgcaa gtggatatca atcattttaa ttgcactggt ttgagcaaaa
    14401 tacaaattta tcgtaacggg ttaaggtagg tgaaatagat gatcgataat ttaataacta
    14461 tttacgataa aaatgacgcg aataatttag ctgaacattt atatgatacg caaggtttag
    14521 gcgctttgtc agactggtta acagctactg ttagcaataa actaaacgga gccgagatat
    14581 ttcagggtac ttattcaata aacggaacta atgcagattt gattgtagac ggacgtatta
    14641 ttcagtgtta tgtagatgaa aatcgagcaa aacagcgtct acggatttat tatgcaaaaa
    14701 cttctgtaat aggcaatacg atagaagtaa aagctgaacc tattttcaat gatataagaa
    14761 aatcggtgtt gaataaatat gacagcggaa cagaaaagat cactgctact caggcatggc
    14821 aaaacgcaaa agttttagcg aaaccagcta tcccttcgca gttttctttc tcgtcattag
    14881 tagatacgct tgctaatgtg aagatagaaa aggcgaattt tttagaattc tttggtggaa
    14941 aagagggatc tattctagat cgatttcatg gtgagtttct aaaagataat aacacattac
    15001 gtcatgaaaa aagtctaggt acggatcata aaatcaaagc gatttatact aaaaacttaa
    15061 ctggtcttga cttagagatt gatgctcaaa gtgttttagt tggagtttat ccattcatta
    15121 gcagctcttc agaaggagaa gacgagatca ctctaccaga agaagttatt ttcacggatt
    15181 acgtggatga ttatcctgct ggatatgttt cttttgttga ttttaaagac aaagcgactg
    15241 atgtagccac attaagggaa gctgctaaag actggttgaa aacaaacata gataaacaaa
    15301 aaccacaagt gagtggttcg attgaattag taccattgag gcatcaaaga ggctatgaaa
    15361 aatttgttga tctagaaaaa gtttcgatgg gtgacggagt agatgtgtat catccacagt
    15421 taaaagtgaa tatgtcagcg agaattgtgg aatatacgtt taatgttcta accaattcat
    15481 acgataaatt agttgtagga aacgtcaaaa caaacttctt agaaaacaca gagaataatg
    15541 tcagcaattt gattaatgat gccattgatc aattgaaaaa cggtggcgaa atcagtgatt
    15601 tactcaatga tattgtagat catcaaactg atatgattac tggtcaaaat ggtggttatg
    15661 ttttattaga tcctaaagaa gcgcctagtc gtattttgat tatggacaca ccagataaga
    15721 ataccgcaag gaatgtttta caaatcaaca atgctggtat tggtttttct aaaactggca
    15781 ttaatggaac atatgaaacg gcatggacgt tagatggcgg attcaatgcc tcgtttatta
    15841 cggctggtga aatagtagga attactatta gaggtactac attaattagt gatggtgctg
    15901 attatagaac aagtattgct aatggcaaaa tgacttggta ctcaaaaaaa gttaacaaag
    15961 atattatgga gctagaagca cgtgattatg taagtgctga tgccggtatt gtatcataca
    16021 ccatgaaaac tggtggtggt ttcatgatta gaaatccaca gggtaacttg gtttttagta
    16081 cgtgggataa tggtaataac agaccgtttc tatcttttgg tgcgcccaat ttcaggtata
    16141 gcaatgctag ttatgtaact tctggcgacg gtagttcttt aagcattaat ggtagtgcgg
    16201 gtaactcatg ggaatttaag gtagctggta ggactatgaa atttactagt gatggtatgc
    16261 taacgttacc aggttgtttt tttggttcat gggaagatgg gaaacttgct aggtttgaac
    16321 aatcaacggt acaagtatat aaagatttta ctgttagagg tactaaaaac tcaactgtac
    16381 caacagaaca ttatggacaa cgactattga acgcttatga aactccagaa tattatttcg
    16441 ctgattatgg ggaagccgtt acaggtgaca atggtaaagt tcgtgttgat attgacccca
    16501 tgtttgctga aacagtaaat ctaagtcggt atatgacaca tgtgacacct acagaactag
    16561 ttttgtgtgc tgttactcat gaagatattg accatttcat cattgaaact agtaagccaa
    16621 acgtattagt tagatggaat ttagtggcac accgtctagg gtatgaagat attagattaa
    16681 aagaggatac agcatatgat agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca
    16741 aggaggtata taaatggcta gcagtttata taatttggct ttagatttca gcaaagaatt
    16801 aaactacacc aaagctatta tggctcgtca aggtgataaa gggattacgg tgacggttaa
    16861 accgtttcta aatggcttgc agatggatac gagtggcgga acatttactt taaaaggaac
    16921 aacaccatct aaccgttacg tagataatgt tgcaactagt gtaactagtg aagaagtcac
    16981 gttttctctt gatggcacat ttatgagtga agcaggatat tataaacact gctacgtaga
    17041 atatagaaaa gacaatcaaa ttttaacaac gcaagatatc atttttttct cactaggagt
    17101 gtctgacatt tcgcaaggcc aagccgatga atatgtttcg caattagaag agttgattcg
    17161 aaagtacaac gaaacttttg atgcttttat ggctgaaatc aaaggtagag tggatagctt
    17221 aaatcaacag attactgatt taactggtca agctaaaacg ctacaagaca agttagatgc
    17281 tctgaaagaa gaaatttcta agttaggtaa cttacaagtg atgtacagta acagcatcga
    17341 cttcggggac tatgattatt cggagaaccc gaatttaatg ccatatatta cagaaccttg
    17401 ggttggacct ttgttaggta atggacatac agttaaagat agtgttaaaa gggttataac
    17461 tcatactaaa actagaacag ctaattccgg cgacatatta tcgcttggct taggtattcc
    17521 gtgtacagcg gaagctaata ataggtatct tataaccact ttacgaccat ctacgacata
    17581 cacattaagc gttactatga gtgtcggttc agattggact ggagagacca atactatagg
    17641 ggttaggctt agatatttaa atgaacaagg tggtatagaa ctgcccatta atgcattaat
    17701 accagcaaat gtagaacgcg ataaaatggt cactcataca tttactggta taacaaaaga
    17761 taatgtaact agcataacaa actgttatgt tgaaatattc tcacttaata gtgaatataa
    17821 aggaacggtt agtgtcagct atgacgttaa gttaaaagca cactacccta acttactaga
    17881 tggcccttac tggttaggta aagttccttt aggcgagaat attgctgacc ctacagttgt
    17941 ctttccacac aaaactagtg aatatatggt ttatggtaga cgcaatacag aaaactatat
    18001 cgctgaccaa acatatacca tatcaatgaa agcaactaaa ctaacagttc aatcgtttgc
    18061 cgtgtatatt gctgctggcc gcgttaaagt aggagacatg aagcctactg aggggttagc
    18121 taatacatgg gagttaactt ttactgtaac taagcaacat atagatagtg gggtaactaa
    18181 ttatctggaa atataccaat acccgtcagc tacaaaagga gctgttcaaa ttgagtggct
    18241 caaactagaa aaaggcaata ctcgaacccc gaatattagt gagtataaat atcgtggtac
    18301 tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac cagaatatgt
    18361 cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag caaacaatta
    18421 taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta atgaagtaga
    18481 caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta tagatgaatt
    18541 agacaataag atcgatgaac gcattaatga tacagctact accacattaa cagttacaaa
    18601 cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta tatattttgt
    18661 ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa ttccagaagg
    18721 ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt ctactttgaa
    18781 cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa actcaagtta
    18841 tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga atcgatatga
    18901 aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg aatgatgaag
    18961 aacatttacc ggaagaatgc acagaagtcg aaccaccgat tccaaattgg aaaccgaaat
    19021 tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta ctggcggaga
    19081 gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag atcagccacc
    19141 gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa tgtttgatcc
    19201 taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt taaacaatct
    19261 caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga tcaatgccaa
    19321 actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag aaaaagcgga
    19381 tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat
    19441 ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt atgtttagtt
    19501 ttagcgatgt gaaaatgatg tatgattggg gctgtttcac taacgaacaa gtaatggttt
    19561 tcgttccgtt gtgcattact gaagaaaaag cagataaaat cattagcaaa gaagagagcg
    19621 catcttaatt gatgtgcttt ttattttgat tcaaggagtt gtcacatgat taatttaggg
    19681 gaatggggag cgatagcagg atcaataacc gctatcgttt ctttgatttt attagtaata
    19741 aaaccaatta ctgcatcttt ctcgaagatt actgagactc tttcaaaagt aagtcacaat
    19801 ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat gattcatgaa
    19861 gaactaaaga aacacgatga aagattagat acacatgcag aaaaattggt agaacacacg
    19921 caacaaatta aaactttatt tagggagaga agaaaatgaa taataaaacg ttcgaagtac
    19981 taaaatggtt cgcactggta attattcccg cactagctac tttcgtgggg ttagttggta
    20041 aagcgctcaa ttggcagtac acagatatct gtgttgtcat cattactggt tttggcgcgt
    20101 ttttagggag tgtgttgggt gtatcaaatc gaacctacaa aatgttctcg gctgaaagcg
    20161 aagaaggagg aaacaaatga aaaagaaaat tactattact gcgatgagcc tgttaacggc
    20221 tcttttttta ttacccatta atacgtttgc ttatactatt aatgacgagt ataatttagc
    20281 gccgaatcaa ggagactcca gattagcaat tcctaacaaa attattttgc acgaaactgg
    20341 aatagatgca ccagcaagaa acgtagccgc caacatgaaa aataattata acggaagcaa
    20401 ttcttatact acagatgtta ttggtgacgg tgggattgtt taccgtgtgg gtgagcaagg
    20461 atatgtttcg tggggagctg gtaacgctaa tccttatgcg cctgtacaga ttgaattaca
    20521 gcgcacatat gataaagcat tgtttgaaaa aaactatcga gcttatattg aatatacaag
    20581 agatagtgca aaaaaatatg gaattccatt gactcttgat caaggaactt ctttatttac
    20641 aaaaggaatc atttctcatt tgtgggtgac aaattatgtt tgggggaacc acacagatcc
    20701 atatggttac ttatcgcaaa tgggagttag caaagaaaag cttgcttatg atttagctca
    20761 tggatttacc gatgaaaatc caacaacttc tgaaaacaag cctgtcattg atccaacacg
    20821 agctggtgca gctaatccta cgctgacaga tggaacaaat cacgcccaca ttgatcagtt
    20881 tggggaaatc gaaaacgcaa acttgcatgt ggctggatgg cacattgcta actataaata
    20941 cgagtatatt ttcattatgg actacaatac tgggaaagaa ttagctcgag taagagctga
    21001 tggaatttat agaccagatg taaaccaagc ttataatact tctggaaatg ttggttatca
    21061 tgtatctttc aatatgcgta attttcctag taagaaagtc tatgtaatga tgcgggcaac
    21121 gaatgatcca gagggaaaca ctaaaggcgg tgcgcaagat ttccatgaca aacgttggta
    21181 tttaaatatt cctaaacgat aaaaatagct cctcgttgag gagcagtaca taactatatt
    21241 gacaactata aaaattattc gataaaatag tgatgttatc gcatatcttc actatcaccc
    21301 ataaatagtc acactccaag ctatgcgata acaggtttgt tgccacacat tctactggtt
    21361 gattgtttat ggctttatgt ggcaacaacc agtaccctta gctcagttgg tcagagcaga
    21421 cggctcataa ccgtccggtc gtaggttcga gtcctacagg gtacattaac gtagccattt
    21481 gaatcgttct gtgttagaat ttttttgaag agtattatac aagctaaagc ttttcttcat
    21541 tgccactcaa atgagtggct tttttatgta tccttttatg gattaatgaa aggatgtttc
    21601 acatagttat acttctgtat atttgaaaag ttttactttg atttttaaat agaaagacat
    21661 ttgggttaaa ttgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc
    21721 ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa gcataccata
    21781 tttttgaaaa aaagtgagaa aaaaggctta caattggagt ggtagttaat tagtgactta
    21841 tttttgattt tatagcactg atactataaa atatagatat catcatatta cacaatctta
    21901 atactaactt aaaaaatatc tcctttcaca agtatggtga taaaattcgt tccgggctac
    21961 ctttttaggt agcctacttt aatctttata cctttctgga tcaacgaaag tatactttat
    22021 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtc
//
LOCUS       DAYYGY010000052        21798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000052 DAYYGY010000000
VERSION     DAYYGY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.053"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..581)
                     /locus_tag="ACHA52_001839"
     CDS             complement(51..581)
                     /locus_tag="ACHA52_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8940534.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(680..1030)
                     /locus_tag="ACHA52_001840"
     CDS             complement(680..1030)
                     /locus_tag="ACHA52_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8940535.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1187..2716)
                     /gene="ahpF"
                     /locus_tag="ACHA52_001841"
     CDS             complement(1187..2716)
                     /gene="ahpF"
                     /locus_tag="ACHA52_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8940536.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2726..3289)
                     /gene="ahpC"
                     /locus_tag="ACHA52_001842"
     CDS             complement(2726..3289)
                     /gene="ahpC"
                     /locus_tag="ACHA52_001842"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8940537.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3433..4095)
                     /locus_tag="ACHA52_001843"
     CDS             complement(3433..4095)
                     /locus_tag="ACHA52_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8940538.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4279..5925
                     /locus_tag="ACHA52_001844"
     CDS             4279..5925
                     /locus_tag="ACHA52_001844"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8940539.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5916..6998
                     /gene="uxuA"
                     /locus_tag="ACHA52_001845"
     CDS             5916..6998
                     /gene="uxuA"
                     /locus_tag="ACHA52_001845"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8940540.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7106..8659)
                     /locus_tag="ACHA52_001846"
                     /pseudo
     CDS             complement(7106..8659)
                     /locus_tag="ACHA52_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8807..9948
                     /locus_tag="ACHA52_001847"
                     /pseudo
     CDS             8807..9948
                     /locus_tag="ACHA52_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(10074..11153)
                     /locus_tag="ACHA52_001848"
     CDS             complement(10074..11153)
                     /locus_tag="ACHA52_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8940541.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11256..11696)
                     /locus_tag="ACHA52_001849"
     CDS             complement(11256..11696)
                     /locus_tag="ACHA52_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8940542.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(11753..12163)
                     /locus_tag="ACHA52_001850"
     CDS             complement(11753..12163)
                     /locus_tag="ACHA52_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8940543.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(12343..12777)
                     /locus_tag="ACHA52_001851"
     CDS             complement(12343..12777)
                     /locus_tag="ACHA52_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8940544.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(12877..13725)
                     /locus_tag="ACHA52_001852"
     CDS             complement(12877..13725)
                     /locus_tag="ACHA52_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8940545.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14114..15880
                     /locus_tag="ACHA52_001853"
     CDS             14114..15880
                     /locus_tag="ACHA52_001853"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8940546.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            15982..17241
                     /locus_tag="ACHA52_001854"
     CDS             15982..17241
                     /locus_tag="ACHA52_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8940547.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            17388..18689
                     /locus_tag="ACHA52_001855"
     CDS             17388..18689
                     /locus_tag="ACHA52_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940548.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            18692..19543
                     /locus_tag="ACHA52_001856"
     CDS             18692..19543
                     /locus_tag="ACHA52_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8940549.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            19556..20371
                     /locus_tag="ACHA52_001857"
     CDS             19556..20371
                     /locus_tag="ACHA52_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8940550.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            20522..21688
                     /locus_tag="ACHA52_001858"
     CDS             20522..21688
                     /locus_tag="ACHA52_001858"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8940551.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         7256 a         3938 c         4017 g         6587 t
ORIGIN      
        1 ttttttggat tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata
       61 gtcatttttc ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata
      121 aagccgaata tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat
      181 ccgccaatca acaacagaat actactataa acactccaac ttttagcttt tgtattcatg
      241 ctgaagaaca actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg
      301 atagaaacga ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga
      361 ccactgaaca gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa
      421 agattcaaca acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa
      481 acagcagaag ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat
      541 aaggcgtatt tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga
      601 cttattttaa cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag
      661 aagaatatct ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta
      721 gctcatcgat cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt
      781 ctactcgtac accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg
      841 cttccgcaaa aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt
      901 gggaataacc cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc
      961 tctccgcttt tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat
     1021 tttgcgccat aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa
     1081 gaaaaagaga agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg
     1141 tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca
     1201 gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt
     1261 tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat
     1321 ttttctcggt cactatttca tttcttggat tcatttcgat cgttctatct agccaatccg
     1381 tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca
     1441 tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg
     1501 tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt
     1561 gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct aacccagcca
     1621 aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt
     1681 taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt
     1741 cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt
     1801 taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa
     1861 cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct
     1921 gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt
     1981 caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg
     2041 catagatagc tgcactacta gctgctggtc caccgccaat cactaataca tcataaggtt
     2101 ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga
     2161 tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga
     2221 tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat
     2281 ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag
     2341 tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca
     2401 aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg
     2461 aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat
     2521 tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca
     2581 tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac
     2641 taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag
     2701 tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca
     2761 atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat
     2821 attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat
     2881 tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta
     2941 atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga
     3001 tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa
     3061 agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt
     3121 gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc
     3181 aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat
     3241 gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc
     3301 caataataat tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat
     3361 attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt
     3421 cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc
     3481 tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc
     3541 aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg
     3601 aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt
     3661 ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg
     3721 gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc
     3781 tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc
     3841 caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata
     3901 cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc
     3961 gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct
     4021 tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg
     4081 caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag
     4141 aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat
     4201 tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa actaatatgt
     4261 tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta
     4321 tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa
     4381 aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa
     4441 atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca
     4501 ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg
     4561 gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg
     4621 tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct
     4681 agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat
     4741 aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat
     4801 acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc ttggccgatt
     4861 gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta
     4921 acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta
     4981 tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct
     5041 tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa
     5101 accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc
     5161 atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact
     5221 gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg
     5281 catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg
     5341 aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt
     5401 gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc
     5461 ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac ctctcaaaag
     5521 gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg
     5581 cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt
     5641 gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga ccttcagcat
     5701 aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct
     5761 cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga
     5821 aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg aaaaacatta
     5881 caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg
     5941 gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga
     6001 ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag aggatcaaag
     6061 aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg
     6121 tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc
     6181 agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg
     6241 tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag
     6301 cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt
     6361 ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct atcaaatcct
     6421 tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc
     6481 tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg
     6541 atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa gatttagatt
     6601 ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca gggagtattg
     6661 cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa agagatcgaa
     6721 tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac
     6781 cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca
     6841 acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa
     6901 gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg
     6961 aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt
     7021 atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca
     7081 ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat
     7141 ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt
     7201 tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca
     7261 attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta
     7321 ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat
     7381 aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat
     7441 acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa ataactggta
     7501 acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt
     7561 ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat
     7621 tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct
     7681 tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc atttctcttg
     7741 aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt
     7801 cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt
     7861 cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata aaaaagtcaa
     7921 tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct
     7981 ctttacatat ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa
     8041 gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat
     8101 aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa
     8161 tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac
     8221 cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat
     8281 agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca
     8341 agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag
     8401 ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt
     8461 ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg
     8521 attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag
     8581 ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata aacgttttaa
     8641 tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa
     8701 tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct
     8761 actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac
     8821 catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca
     8881 cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat
     8941 tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca
     9001 aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta tactacaatc
     9061 agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg agaacacttt
     9121 gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt
     9181 aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatttaac
     9241 gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag gaggagatct
     9301 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
     9361 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
     9421 tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
     9481 aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagatac
     9541 agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa aaccagatgt
     9601 gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa
     9661 aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa ttctaaaaat
     9721 aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat cagctgaaac
     9781 cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa acagctacca
     9841 aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct attaagtata
     9901 gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca tttgaataat
     9961 gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc aaaataccag
    10021 attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt attttaaggg
    10081 atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac atctaaaaag
    10141 aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca
    10201 agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat caacttcgga
    10261 ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat ataaacaaca
    10321 tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga aatgataaca
    10381 cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag cagctgatga
    10441 aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc gacaacaaat
    10501 tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata gtaaatatct
    10561 tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat tgtttttttc
    10621 ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac tgccattttt
    10681 cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa
    10741 tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc ttttggctga
    10801 tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat aagcatcggg
    10861 atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat catgatagct
    10921 aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa
    10981 taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa gaaaagaaca
    11041 aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa aaaacgctga
    11101 taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt cattatatga
    11161 tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag
    11221 ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg gtcttgatga
    11281 aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt
    11341 ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa cgcagcacct
    11401 tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg acgattaata
    11461 taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc cagacacaaa
    11521 tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa catcacaaga
    11581 tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct aatgatttct
    11641 ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc ttccattttt
    11701 ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa gtctattcac
    11761 taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac ggacacactt
    11821 tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg atagatcctt
    11881 caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa acttgtgcac
    11941 tgacagtaga agccccagag atattttttt gctgtttgat caattctaat gcactgttaa
    12001 aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt
    12061 taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact tctccagagc
    12121 gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaagtta ccacctttaa
    12181 ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag aaatggattg
    12241 gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat agcaaacata
    12301 tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat atttctcaaa
    12361 aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca tccataaata
    12421 accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt ctgtcatttc
    12481 ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga tatcagagac
    12541 gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca aaccaagaga
    12601 cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga cttcattatt
    12661 gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg tttgatacag
    12721 ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt tatgcataaa
    12781 gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc agctaaagaa
    12841 aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat gataagtaaa
    12901 tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg gatcgatttc
    12961 ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca ataaaacctc
    13021 ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac gtccaatctc
    13081 agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca taacagattt
    13141 ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg caatagataa
    13201 aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa caacgaatgc
    13261 gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa aaatgagggg
    13321 agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc
    13381 aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca ttgtttctgg
    13441 aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt
    13501 tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta aaataactat
    13561 agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga cacctccagc
    13621 agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga agagggcaaa
    13681 aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt cacttcctta
    13741 attcattaat aatactctta tcttattata acttactttt caccttaagt taactttaat
    13801 taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa tatacttcta
    13861 cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat cgcttaagat
    13921 aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt tttaagtcta
    13981 tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac cgataacatt
    14041 tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat attaaaacac
    14101 aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg aatatgcatt
    14161 tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa
    14221 agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt atcaaaaaca
    14281 acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa ttgaagtgac
    14341 gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata acacggactg
    14401 tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg aaccaaattt
    14461 ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag aatgggtaaa
    14521 agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca aagcaaatga
    14581 ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact ttttcggggg
    14641 agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca tcaatggcat
    14701 ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga ttgattatta
    14761 tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg atgaagcaca
    14821 taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg tccattcacc
    14881 acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact ggttccacat
    14941 ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg ctgaaaatat
    15001 cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag caaatccaga
    15061 agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg atattgatgg
    15121 atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat tcagagaagc
    15181 agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt cctctcaagc
    15241 ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag attcgattaa
    15301 agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca
    15361 gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag attctcatga
    15421 tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc
    15481 ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa tagggatgac
    15541 tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg aacaagatct
    15601 agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt ctaagattat
    15661 ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc tctatttcac
    15721 acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag aaccttgggt
    15781 agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag aagacgggaa
    15841 agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac cggtaacaac
    15901 aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa cagcacgttg
    15961 aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg tttagggtta
    16021 atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc ttcagcagaa
    16081 ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata taaagattat
    16141 ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa
    16201 aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg aaagggacct
    16261 gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg acatctggcg
    16321 gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca agtgacttat
    16381 gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta ctataacaaa
    16441 gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc caaagacagt
    16501 cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa atggactgac
    16561 ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg tgatgatggc
    16621 acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat ctcttatgca
    16681 acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag tgcaggagac
    16741 ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg tccatggcaa
    16801 gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc tactctaaat
    16861 aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag caattatgct
    16921 aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga aaatcaagaa
    16981 aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga aacagcaaag
    17041 gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc tcaagcgatg
    17101 ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat gttcgatgca
    17161 gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa
    17221 gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa aaatggaaca
    17281 ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct tgttgccatt
    17341 atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg cagcaagata
    17401 aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca gggatggggc
    17461 agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc gtcctattca
    17521 ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact ttgggaagta
    17581 taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa ttcatcatta
    17641 caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga gtagcgaaga
    17701 tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat aatataggtg
    17761 atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt tttacgatca
    17821 ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc aatcatattc
    17881 caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc ttctttttaa
    17941 gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg actttatgtg
    18001 caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac cagaaattta
    18061 ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc ccagctttta
    18121 ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc aatactcaag
    18181 tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct tggaaaacgg
    18241 acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga tttccctata
    18301 tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat gaagcagcaa
    18361 aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct gctgtcttcc
    18421 tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat ttctccatga
    18481 tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt tctactgata
    18541 ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat tctatggctt
    18601 cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa
    18661 agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga gtgctcgatt
    18721 ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt cagtcattat
    18781 catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata ttgcagcttt
    18841 tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat tcagtgaaac
    18901 actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa tgatcgtaca
    18961 agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta tcggccgcaa
    19021 atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag cattgactgc
    19081 cttttatgtc atggctttct tattagaagc gctcgataag tactggttct tgacaatgat
    19141 ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact ttgacactgt
    19201 accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca ggattttttt
    19261 ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat gggcttttat
    19321 gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg aaaatcagac
    19381 tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag tctctctttt
    19441 tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct ttttacaaaa
    19501 gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga gtatcatgca
    19561 aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt ttgcgggacg
    19621 caaactatta tcatggccta aatttttctt gatatttgta tttttagttt ccttgatggt
    19681 catgcctgtg actctttttt atgccaatca gatccaagcg attccattag aacaatttct
    19741 atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac ttgaattatc
    19801 tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa ttttggtagg
    19861 tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt ttgagaagga
    19921 acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga tgaattatag
    19981 cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat ttttagaaag
    20041 agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa gtcttggtct
    20101 tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct tgtggatgac
    20161 aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt tgatgctgaa
    20221 tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact ttgactttat
    20281 cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg tttttgtcaa
    20341 aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa
    20401 tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt
    20461 agttgattct tttctttttt agtattaacg taatagggta gtttttttaa ggaggaaaat
    20521 catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc tccctgtcga
    20581 agtaaattat tgggacggta caacgaaaca atacggagat acggacactc ctccccaaat
    20641 caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg cttctttggc
    20701 actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc aagcattgat
    20761 caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc taaaatggct
    20821 gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg cgcattatga
    20881 cttaggtaac gacttttata aactttggct agatccaact ttgacttatt cttgtgctta
    20941 tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc atcatatctt
    21001 agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg gctggggaac
    21061 attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca cactaagtga
    21121 agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag ataaatgccg
    21181 tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt
    21241 aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga
    21301 tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac aaggcggtgc
    21361 gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg gtgttactga
    21421 attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa gcttacgtcg
    21481 tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc aagatcaagt
    21541 cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac aagcttgtgc
    21601 agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc aacctggaaa
    21661 caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa agactattta
    21721 aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac ttctaggatt
    21781 gtctctaaag cttagttt
//
LOCUS       DAYYGY010000053        21484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000053 DAYYGY010000000
VERSION     DAYYGY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.054"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..1775
                     /locus_tag="ACHA52_001859"
     CDS             114..1775
                     /locus_tag="ACHA52_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8940552.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1845..2192)
                     /locus_tag="ACHA52_001860"
     CDS             complement(1845..2192)
                     /locus_tag="ACHA52_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8940553.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2276..3217
                     /gene="opp3b"
                     /locus_tag="ACHA52_001861"
     CDS             2276..3217
                     /gene="opp3b"
                     /locus_tag="ACHA52_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8940554.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3218..4261
                     /locus_tag="ACHA52_001862"
     CDS             3218..4261
                     /locus_tag="ACHA52_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940555.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4267..5337
                     /locus_tag="ACHA52_001863"
     CDS             4267..5337
                     /locus_tag="ACHA52_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940556.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5327..6280
                     /locus_tag="ACHA52_001864"
     CDS             5327..6280
                     /locus_tag="ACHA52_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940557.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6292..7302
                     /locus_tag="ACHA52_001865"
     CDS             6292..7302
                     /locus_tag="ACHA52_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8940558.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7587..8408)
                     /locus_tag="ACHA52_001866"
     CDS             complement(7587..8408)
                     /locus_tag="ACHA52_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8940559.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8505..8783)
                     /locus_tag="ACHA52_001867"
     CDS             complement(8505..8783)
                     /locus_tag="ACHA52_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940560.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9051..11060
                     /gene="metG"
                     /locus_tag="ACHA52_001868"
     CDS             9051..11060
                     /gene="metG"
                     /locus_tag="ACHA52_001868"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8940561.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11539..12126)
                     /locus_tag="ACHA52_001869"
     CDS             complement(11539..12126)
                     /locus_tag="ACHA52_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940562.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12301..13623
                     /locus_tag="ACHA52_001870"
     CDS             12301..13623
                     /locus_tag="ACHA52_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8940563.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13778..14554
                     /locus_tag="ACHA52_001871"
     CDS             13778..14554
                     /locus_tag="ACHA52_001871"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8940564.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14547..15116
                     /gene="rnmV"
                     /locus_tag="ACHA52_001872"
     CDS             14547..15116
                     /gene="rnmV"
                     /locus_tag="ACHA52_001872"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8940565.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15109..15993
                     /gene="rsmA"
                     /locus_tag="ACHA52_001873"
     CDS             15109..15993
                     /gene="rsmA"
                     /locus_tag="ACHA52_001873"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8940566.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16444..17073
                     /locus_tag="ACHA52_001874"
     CDS             16444..17073
                     /locus_tag="ACHA52_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940567.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17197..18306)
                     /locus_tag="ACHA52_001875"
     CDS             complement(17197..18306)
                     /locus_tag="ACHA52_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940568.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            complement(18650..19969)
                     /locus_tag="ACHA52_001876"
     CDS             complement(18650..19969)
                     /locus_tag="ACHA52_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8940569.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            20199..20621
                     /gene="mgsA"
                     /locus_tag="ACHA52_001877"
     CDS             20199..20621
                     /gene="mgsA"
                     /locus_tag="ACHA52_001877"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8940570.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      20808..20906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            20944..21480
                     /locus_tag="ACHA52_001878"
     CDS             20944..21480
                     /locus_tag="ACHA52_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8940571.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6950 a         3868 c         4381 g         6285 t
ORIGIN      
        1 tttatgaaaa agacaaaaaa aagtgttgat tttttaataa ttattcatta aactcttaaa
       61 caacttcaga aaatattaat tattctgaaa attccaataa aggggtacgt aaaatgaaaa
      121 agaaatttac atttggtttg gttgcggtat gcggccttgt acttgctgga tgttatggcg
      181 gcagttcagc cacaagcagt agttcagcaa aaagcggttc agcagatgga ggaggcgtat
      241 ttaacttagt cgtgcctcaa gaaatgccta cagctgactt atcagttgca acagatacaa
      301 tcagttttac agcattgaat aacgtttatg aaggaattta ccgtcttgat gaagacagca
      361 aaccacagcc ggcaggagct agcgagttgg cagaagtcag tgaagacgga ttaacttaca
      421 aaatcaaatt acgagaagac gcaaaatggt caaatggtga tcctgttaca gcagcggatt
      481 acgtttatgg ctggcaacga acagtagacc cagctacagc ttctgaatat gcttacctgt
      541 atgcgccagt tgaaaatgca gaagacatta cagctggaaa gaaagataaa tcagaactag
      601 ggatcaaagc agttggtgac tatgaattag aaatcaaatt gacgaaacaa acaccatatt
      661 tccaatatct gcttgctttc ccatcatttt tcccacaaaa acaatccgta gtggaagaaa
      721 atggggatgc ctatgcatct gcaagtgata aagctgttta taatggacca ttcacattag
      781 aagattttga tggaccagga acagatacag aatggactta taagaaaaat gatcattact
      841 gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta
      901 ctgcattgaa cttattcaaa gatggccaag cagatgacgt gatcttgtct ggtgagttag
      961 cacagcaaaa cgcaaacgat ccagcttata cttctgttaa agaagcgcgg acaagctata
     1021 tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa tgtgaaccta agaaaagcta
     1081 tttcttattc tatcgatcgt gaagctttag taaaacaagt actaggtgac ggatcagttg
     1141 tctctacagg attgatccca tcagatatgg taaaaagtcc aacaacgaac gaagactttg
     1201 caaaagaagc tggaaaatta gtttcttatg acaaagataa agcaaaagaa tattgggaaa
     1261 aagcgaaaaa agaattgggc gttgattctt tagaatttga tttgatggct tctgatgatg
     1321 attcatcgaa aaaagtaatc gaatatgttc aaaactcaat ccaagaaaac ttggatggcg
     1381 taacagtcaa accaacacct gttccattct ctgtacgtct tgaccgctca acttcgggtg
     1441 attttgacgc agtactaggc ggatgggctg ctgactatgc agacccaagc agctttactg
     1501 atttgtttgt cacaggtaat tcatacaacc gtggtcgctg gagcaatgca gaatatgacg
     1561 aagcagttaa agcagcttca tcaacagacg ctggcgatga acaagcccgt tgggatgatt
     1621 tccaaaaagc aatcaagatc atctctgatg atatgggtgt cgttccggtg taccaaaaag
     1681 cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt
     1741 catgggatta caaatggaca tacgttgaag aataatcgtt aataccgtga gcatgagaaa
     1801 aaacgaggct gcggcaaatg tcgcaacctc gttctcatat tcacttatcc taacaaagaa
     1861 aaaagcatga aaatagcaga aagagcaatt agtattccgg caatgatcag ccaaaagctg
     1921 tacataccgt agctggcaga cgagagcgag gaaaattctg gttttttctt tcggttcctt
     1981 gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat
     2041 aaaaaaccgc cgataatcag aaacggcaat gaccataaga atagatcatt agataattga
     2101 atgagtgtaa aggaatggtt gatgagattt tttattaaaa taaccagaat cagtagaaaa
     2161 gctactgcgt aggggataga tttttttttc atttgattgc tcctttattt ttggttcatt
     2221 atctaataat gtagcgaaac atctgtcaga agtcaaaagc tagggggaaa cattagtgaa
     2281 tagttatatc aaatatgtat taaaacgtgt ctttttcatg atcatcacgc tttggttaat
     2341 tgcaacaatc acctttttct tgatgcaatt gctgccgggc acgccatata cgaatcagga
     2401 acgtttgagt cctgaaacaa tcgagatgct aaataaacaa gtcggtttgg ataaaccagt
     2461 tatcgtacaa tatgggattt atctttctaa tttgcttcaa ggagattttg gaatttcctt
     2521 ccaatttaaa aaccagccag tcgcacattt gttagctgga agaatcggac catccttgca
     2581 gttagggtta caggcaatta tctttggaac agtcctaggg acgattttag gaacgatctc
     2641 tgccatgaag caaaatacat gggcagatac atcttctaca ctcgtagcga tattaggtcg
     2701 ttccattcca aactttgtat ttgctgtttt attacaatat atctttgcaa tcaaattaca
     2761 agttttgcca atcgcaaaat gggatggctt tgtgtacaca atcttaccaa caattgcgtt
     2821 ggccatgtcg ccgcttgctg actctgctcg gtttatccgt acggaaatgg tggaagtact
     2881 gcatagtgat tatgtggaat tagcaaaagc aaaaggattg agccgttggg aaatcgcctt
     2941 taaacatggt cttcgaaata gtttgattcc attgatgact ttattaggac cgttagctgt
     3001 agcattgatg accggatcac tggtggtaga aaatatcttt gctattcctg gtatcgggga
     3061 acaattcgtt aagtcaatca caacaaatga ttatcctacg atcatggctg taacgatcct
     3121 ttactcattt atgctgattt ttgttatttt gatcgtggat ctattgtacg gtctagttga
     3181 tccaagaatt cgtgtttcag aagggagccg aggataaatg gaaatgatac caaagcgtta
     3241 tgaaacagtt gcagatatcc ctgcggatga atttcttcca ttacaaaaaa ataccgaaga
     3301 agaacgtgaa cagattactg caccttcttt gaattttatc caagattcat ggcgccgttt
     3361 aaagaaaaac aaagctgccg ttatttcaat ggttttacta ttggtcatca ttttgatctc
     3421 tgtgatcaca atctttgttt ctcctcatga tccaactgcc caaaacgtgg cttatatcaa
     3481 tttgcctcct cgtattccgg gaatcaatat cgatggattg aatggaaaag caatggttgg
     3541 tggtgaactt gttgataaat atgctcaagc aaacgtccct tcaaacgtaa atttcttttt
     3601 aggaacagac ggtttgggac gtgatgtatt aagccgctta tttatgggaa tgagaatctc
     3661 gttgctgatc gcatttattg ctgcattact agatgtaacg attggtgtag cttacgggtt
     3721 ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg caacgttttt tggaaatctt
     3781 atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat
     3841 tttttcaatc gtggctgcta tggccatcac aaactggata ccaatggcac gtatcgttcg
     3901 tgcacaaaca atgaaattaa aagatcaaga atatgtttta gctggattga cattaggtga
     3961 atcaagaatg aaaattgcat tcaaacatat tttgccgaat atctctagtg tcattattat
     4021 tcaaatgatg tttagtattc catctgctat tttcttcgaa gcctttttaa gctttatcgg
     4081 tttaggtctt acgcctccgt cagcttcatt aggaacgatg ttgagcgaag ggtacaagac
     4141 attcctgtat ctgccttatc tattatggat tccggcagta actttatcgg ttatcatgat
     4201 tggctttaac ttacttgctg atggattgcg tgatgccttt gatcctaaaa tgaaagagtg
     4261 aagacgatga cgaaaatttt agaagtgaaa gacttagaaa tctcttttga tacgtatgct
     4321 ggaaaggtac gagcgattcg cggtgtcaat tttcatttga ataaaggaga aacattggcg
     4381 atcgttgggg aatctggaag tggaaaatca gtgacgacca gaagcatcat gcgcttgctg
     4441 tcaagcaacg ccaatataga tgctggtcaa atcctcttta aaggtaacga tatcgttgga
     4501 aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat
     4561 ccaatgacct ctttagatcc gacgatgccc attgggaagc aagtcgctga gtcccttaga
     4621 aagcataata aagtttcaaa aaaagaggga caaaaagctg cattagaatt gcttaaatta
     4681 gttggaatac ccgaagctga gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt
     4741 cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt atccggaaat cctgattgct
     4801 gatgaaccta caacagcgct tgatgtgact attcaagcac agattttgga gctgctgaaa
     4861 gaaattcaga caaaaatcga tacatcaatt atctttatca ctcatgacct tggtgtagta
     4921 gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa gaatcgttga agttggaaca
     4981 tcggaagaaa ttttctacaa cccgcaacat ccctatactt ggggactgct tggttcaatg
     5041 ccgacattag atagtgcgaa cgatcgattg tatgctattc ctggttctcc accggattta
     5101 ttgaacccgc caaaaggaga cgcattctat ccaagaaatg aattcgcgat gaaaatcgat
     5161 gcagaacaag aaccaccatt ttttgaacta tcaaaaacac acaaggcagc aacttggcta
     5221 cttgctccgc aggcacccaa agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc
     5281 tttaaagaga aacaaaaaca tacttacggt caaggaggaa ttgctgatgc caaataaact
     5341 acttgaagtt aaaggattga aacagtattt caatgtagga aaaaagaatg aagtacatgc
     5401 tgtaaatgac atcagctttc acatttatga aggagaaaca ttcggtttag ttggagaatc
     5461 tggaagcgga aaatccacta ctggccgtac gatcattcgc ttgaatgaac ctactgatgg
     5521 tgagatatta ttcgacggtc aagatataac gaagatcaaa gataaaaaag gtttgacgaa
     5581 attccggcat gatgttcaga tgatctttca agatccatac gcttcgttga atcctcgtat
     5641 gaaagtcaga gatatcattg ccgaaggaat tgatgttaat ggactagcta aatcatctga
     5701 agagcgtgca aaaaaagtcg atgatctatt gagaactgtt ggattgaatc caagccacgg
     5761 tactcgttat cctcatgaat tttcaggtgg gcaacgccag cgtattggta ttgcacgagc
     5821 attggcagta aaaccgcgtt ttatcatttg tgatgaacca atttctgcac tagatgtgtc
     5881 gatccaagcc caagtggtca atttgttgca agatctccaa aaagaacatc aattgaccta
     5941 tctatttatt gcacatgatt tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat
     6001 gcataacggc ttgttattag agatgggaac aagtgatgaa atctataatc acggtgttca
     6061 tccttataca gaaagtttgt tatctgctat tcctctacca gatccagacc atgagagaaa
     6121 acgcagaaga atcaaatatc aacctgaacc agatgacgga caagtacgtc aacttcgtga
     6181 aatcgcacca gaacattttg tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa
     6241 actaaaacga caaaaggaat ctcttctggt tgctaactag gagggacata gatgctaaaa
     6301 gcttttaaat tccgtatgta tccgacagaa gaacaaaaac agcaattgat tcgtactttt
     6361 ggatgtgctc gatttaccta taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa
     6421 acaggagtag ccaatttttc gctcacaccc gctacattaa aaaaagaata cccattctta
     6481 aaagaagtag acagtcttgc tttagcaaat gcacagctta atttggatcg ggcttttcgc
     6541 aattatttca agggacgtgc cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa
     6601 tcttatacga ccaacaacca aacccgtacg atctatctgg aaaatggcta cttaaagtta
     6661 ccaaaacaaa aagaactaat caagataaat cagcatcggc cagttgaagg atcgattcgc
     6721 tccgctacga tttctgctcg atataatgaa gaattttatg tcgcactact ttgtgatgtt
     6781 tcgccagtaa aaaaagaatc tttagctaaa tggatcggga tcgcctatca tccgaagaca
     6841 ttgatccaaa catctcgacc tctagaagta actttaccaa aatttcacca gacagaagaa
     6901 aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc gctccgctca ccatagaaag
     6961 atcaggttag atcaggcaag caattatcag aaacaaaaga gaaaagtaat ggatctttat
     7021 ctgaaacaaa agaaccagcg agaagattat cttgaacaat tatctgggaa attggtcaaa
     7081 caatatgact acttatttgt agaatctttt ccaaaagaag aggcacatgc tgatttttct
     7141 attcacgact ggcataaatt aataacaaaa ttgcgttata agtcccaatg gtataataaa
     7201 aaatttcttt tgatcaatac cgacggagca gaagaaagca attccgtaag aaagagccaa
     7261 gtagtagaaa aaatgggcag acattcggtt atcaagggat aaaaaatgaa tgaaactgat
     7321 aggagcgtta gggttagcct tgttaataaa aagtcttaat gacttttgtt tctaggaatt
     7381 tttcttttga agaaaagctc actaattatg acaaaccaat aaaacaatag cagaaaaagt
     7441 tcgtacgagc tttttctgct attgttaatt ttttattcag aaagtttacg aatacatcaa
     7501 aattctgaaa caaaaaacgt aactcacaaa gagttgtttt tgcgtttttt cttgaatgat
     7561 taggtctgaa tcttttgctc acaaccttat tcatggtgcc ccggcttgag ctgatagatt
     7621 tttgaaggac ggccgattcc tacaggacgt tcaccgatct catcgaaata ttggagcata
     7681 gctttcttaa aattggaatg atcgatcgtt ttataatcga tccctaagaa tttagcaaat
     7741 acttttcgag cttccgtaat cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg
     7801 tgttccattt tattgactac acggttgaat gctttcaaga tgatttgact atggtcaaaa
     7861 gccaaagtat ctttgccaga agactctcca gtgactaaat caagcgagat agccacttct
     7921 ccgctagata aatggatctt gttactatga cgttctagtg tgaaccagcg tacttccttg
     7981 gcatcatctc cggcgattag cggttcttct ccaataaagg ctaagtagct gactgtgacg
     8041 acccagcctc gcggatctcg attaggcgta ctgaatgtat gcagttgctc aatattttct
     8101 tttgaaataa ccacaccagt ttcttccttt gtttctctta agacgctttc tccagttgac
     8161 tcatcctttt ggacaaagcc accaggcaat gcccatgaat ttcgataagg atgccctttt
     8221 ctttggatca acaaaacttt cagctgatct tcttctttgt tatagcacat cagtacgata
     8281 tcgacagtca aagagggctt gtcatattct ggacgttcct gtttttgata ccagttaaga
     8341 aattcttctt cacttgcttt atgttcataa tataattttt cttcttcttt agaagcaaaa
     8401 cggttcatct gaccacctcc attgacagta aaagtacttt ttaccttatt gtagcagact
     8461 ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta tcatttaaat atgaaaaata
     8521 aatggaagta tgagattgaa taaaaagaaa agcagataaa acggcaatac tttataccgc
     8581 tcttcacgaa tggcaaagaa gccacaaata acgatatata cgtcaatgat aaaacgcaaa
     8641 atccaagctg ctgtcataaa actgttcaaa tgcccatgga ggttaaaata accatacata
     8701 cgaatataaa aatagctgaa tattaaatgt gctaatgaaa gaaaagcaat actaagtgta
     8761 aaaaaaagat gtttcttctg catgattgtt ccagtccctt actaagatag atagtcttta
     8821 ttataccacg tgtgagggga gaaataaaaa gccgtttttt agtagatatg aaacaaaaca
     8881 aaaacccgca tttattcaac ctattcatcc ttttaaagga tagattgaac gtagatagaa
     8941 gtcgtttatc cagtcttttt cattttgaaa aaaagctaac agcatgcttt ttttatggta
     9001 tactagcaag gaaacgaatt cgcatcatgc gataaaggag gaagagcccc gtggcagaaa
     9061 aggaaacttt ttatatcact acgccaatct attatccaag cggcaaactt catatcggca
     9121 actcgtatac tacgattgca tgtgacgcaa ttgctcgtta taaacggttg atgggctttg
     9181 atgtgtttta tctcactgga gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag
     9241 aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc ggctgatgtc aagaaattat
     9301 ggaaaacgct tgatatcagt tatgataaat tcattcgcac gacagatgat taccataaag
     9361 cagctgttca aaaaatattt gatcgtttat tagaacaggg agatatttac ttaggtgaat
     9421 atgaaggctg gtactctgtt tctgacgaag aattctttac agaaacacaa cttgcagaag
     9481 tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag
     9541 aattggtaaa agaagaatca tacttcttcc gcatgagcaa atacgcagac cgtctattgg
     9601 aatactatga agaacatccc gaattcatcc agccagaatc tcgtaaaaac gaaatgatca
     9661 acaactttat caaaccagga ttagaggatc ttgcagtttc acgaacaact tttacttggg
     9721 gagtaccagt caagagtgat ccgaaacatg tggtgtatgt atggatcgat gcattatcca
     9781 attatattac tgcactagga tatggctccg aagatgaaag tctattcgaa aaatactggc
     9841 ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa
     9901 tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt atttgggcat ggctggctat
     9961 tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg
    10021 tggaacgtta tggtttagat gcactccgtt attatttgct tcgtgctgtt ccgtttgggt
    10081 cagatggtgt ctttacgcca gaagattttg tttcccgtgt caattatgat ctggcaaatg
    10141 atctagggaa cttattgaat cgaacgattg ctatgatcaa caaatattgt gatggactcg
    10201 ttcctgacta tgcctcactt gtcactccat ttgatagtga actgtctacg acggctgcta
    10261 acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga attcaataca gcattggctg
    10321 aaatctgggt gctgatttct cgtgcaaaca aatacattga tgaaacagaa ccatgggtat
    10381 tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt catgatccat ttagctgaat
    10441 ctttgcgtat cgcagcgata ttacttcagc caatcatgac agaaacgcca gagaagatct
    10501 tcaatcaact aggattggac tctgaaacaa tgaatcttga aggattacac tttggcgaat
    10561 ttccttcagg aacaaaagta gttgcaaaag gtacaccaat cttcccacgt ttagatatgg
    10621 aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg tactcaaaca aacgaagata
    10681 cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat
    10741 ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt catcaactgt caaaaagtag
    10801 aaggggcaga taagttgctg caattccgac tagatgctgg agacagccaa gaccgtcaaa
    10861 tcttgtcagg aatcgctgaa ttttatcctg atccaagcga attgatcggg aaaaaagtcg
    10921 tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc
    10981 tttctgcaga agcaccagat ggctctttac aagtaatcga agcaccaaaa tccatgccaa
    11041 atggctcaga aatcgcatag aaattcaaag ggggtatgac actagttgtg tcataccccc
    11101 tttttccgaa atttgagaag caattttcgt aaattccttc ttattacttg aagccaatca
    11161 tataatacag aaacagagaa ctatccaaaa taaatcctag aaaaagaagt acgagcttcg
    11221 ctgatttttg ttaatttaga ataatactaa taggaatcgt aaaaacagaa aaaaagcata
    11281 atgataaagg gtcgggcaga ttttaattga ttcatctggt actctttttt attgtcctta
    11341 cattcataaa gtttgaatgc aatgacttta ttacatctat atttatatct aacagtagcg
    11401 ttgatgtgtt aattataagt tctatcactt tatgttcaat tagtttgtgc tcttatatgt
    11461 gtgattgaaa atatagataa aaaaacaatc atcggctttg acttcgatga ttgttttctt
    11521 tattttgtaa aaatcctatc acttgatttt atggtaacct tttatttttg tcttcaaacg
    11581 gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt
    11641 acgtccgata ccataatgtt gatagagctt tgtcaacatc acgtgtaaca agatcaaccc
    11701 taaaagcaag gagccagcta taaaccaatg attatcccaa tgcatgactt cactaagtgc
    11761 catcactcca aaaggataga aataaaaata gatcccgact aaggtcatcc gccagttctt
    11821 tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg
    11881 aaaaagccca accaaaaagg aatacaacat cccccacaag atcatcacag gacctttgat
    11941 caatgcaacg attgcgatga acaaagtcag caatattagt ttttgtgata acgttgcaac
    12001 agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt
    12061 acgggaaaaa tcgacccggt tggataaata atctgctaat ttcggataag aacgcttaaa
    12121 atccataaac tgttcctcct tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag
    12181 aaatacatcc gtcttaagag ggagtttttc actgaaagtg gagaaaaaag aatggtaaat
    12241 taaaatattc tcagttatac tgaaaattat accaaatttc aaagaagaaa gaggatggga
    12301 atgaaaggca aactaaaacg agaaatcaat ctgtttggcg cattagctac cgttatggga
    12361 accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg ttactgctag tactcagtct
    12421 acaagtttga cattgctagc ttggcttttg ggtggtttct taacgatctg tgctggattg
    12481 acagtggctg aattagctac agcaatcccg gaaactggtg gagctgtaaa atatattgaa
    12541 gctgcgtatg gaaaattgcc cagcttttta ttaggatggg cgcaaagctt gatttacttt
    12601 cctgctaata ttgctgcatt atcgattatc tttgcgacac aattgacgaa cttattacaa
    12661 ttatctaccg attatctttt gttgattgca gtgatcaccg ctgtttctgt gacagggttg
    12721 aatttgttag ggacaaaagt cggtgcttct gtacaatcag tgacgttgat tgttaaatta
    12781 ataccaattg ctgtgattgt catatgggga ttattgaccc caggacaagg aactgtccag
    12841 ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg aaggattgag cagtgcgttg
    12901 ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg
    12961 aaacggccgg aaaaagattt gcctaaagca attattcttg gtttgagttt tgtaacagtc
    13021 gtttatctat tgatcaattt tgtttttttg aaaactttgc cgattgatca tcttgccggt
    13081 aatttgaatg cagcttctga agcatcagac gtcatttttg gaggaatcgg tggaaagctg
    13141 gtcactattg ggatcttgat ctcggtatac ggtgcattga acggttatac actaactggg
    13201 atccgcgtgc cttatgcgat ggctttagaa gacgagctac ctttcagtaa acagctgaca
    13261 aagctttcaa agaagtttac ggttccttat gttcctgcgg ttttccagtt agcagtagct
    13321 tgtattatga tgagccttgg ttctttcgac tttttaacag acatgttgat ttttgtgatg
    13381 tggctattca gtttactgat ttttattggt gtctttattt taagaaaaaa agcgccggaa
    13441 ttgccacgtc catataaagt accactttat ccaatcgttc cgattattgc gattctcggt
    13501 gctattttca ttttaggtat gacgatgatg acgcaaacga aattagcatt gatcggtata
    13561 ggtgttacct tgataggtat cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag
    13621 taaaatattt caacaacaga cagaaaaagt acgaactagg tcctctagat cttcgtgctt
    13681 tttctatttc aataaaacaa gttcttctat tttgattttt gctacttctg caagtagtgc
    13741 ttatgtgcta aagtaagaat gaaaaggaga gataaacatg atatttgatt ctcataccca
    13801 tttaaatgct gaacagttca atgaagatat tccagaaacg attcagcgag cacaagaaat
    13861 gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca acgattgaaa agtctttgca
    13921 attaagccat gactatccaa atatatacag tattatcggc tggcatccga cagaggctgg
    13981 cagttatacg aaagaaatcg aaaataaatt acaagaacaa ttgactttac caaaggttgt
    14041 agcattaggg gaaattggct tggattatca ttggatggaa gacccgaaag aagtccaaga
    14101 taaagtattt cgcagacaga ttgctatcgc aaaagaaatg aacctaccaa tcagtattca
    14161 tactagagaa gcacttgaag acacgtatca aatcctaaaa gaagaagatg ttcgagatat
    14221 cggtggaatc atgcatagtt tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt
    14281 agggatgcat atttcattta gcggtgtcgt cactttcaaa aaagcattag acgtccaaga
    14341 ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa acagatgccc cttatctggc
    14401 accagttcct tttagaggaa agcgaaatga accaggctat actcgttata cagtagaaaa
    14461 gattgctgaa cttcggaatc ttccgacaga agaagtggct gcccaaacat ggacaaatgc
    14521 gcaccgatta tttggtctaa gcgaacatgg ataagatgaa gatccaagag attatagtcg
    14581 ttgaaggaaa agacgataca agaaggctgc gggaagtagt tgatgcagat acgatagaaa
    14641 caatagggtc tgcaatcaat gaagagatcc tgatgcaaat tgaacatgcc caagagactc
    14701 gtggagtgat cgtttttacc gatcctgatt tttcaggaga aaaaataaga aaaatcatta
    14761 tggaagctgt tcctcaggca aaacatgctt ttttgccgcg cacacaagct aaaccaaaga
    14821 aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa
    14881 aggtcgtgac gccagacatt tcccaaacgg ctgtttctga aatcactaga caagaccttt
    14941 tagactatgg tttaatagct ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg
    15001 agttgcgcat cggttacacg aacggcaaac aattggaaaa acgtttgaag atgtttcgta
    15061 ttacgccgaa agaactcgcc caagcaatga aaatagtgga ggaacaacat gagtgaatat
    15121 cgtgaaattg cgacaccctc tagaacaaaa gagattttat tgaaacatgg tttttcattt
    15181 aaaaaaagtt taggacaaaa ttttctgact gaaccaaata ttttaaggaa aatcgtagaa
    15241 actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg gaccaggaat cggtgcattg
    15301 acggaacaat tagccaagca tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg
    15361 atccctgtat tggctgatac gatgcaaccc tatgacaatg tgactattgt acatcaagat
    15421 attttaaaga ccgatttgtc aacagctgtt agagaaacat ttcatgagga gcttccgtta
    15481 aaagtcgtag cgaatttacc ttactatatt acgacaccga tcatgatgca ttttcttgaa
    15541 tcagatttgg ttgtggatga actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc
    15601 tctgctgaac caggaacgaa agcttatggt tcattatcga ttgctgttca atattatatg
    15661 gaagccagtt tagcattcat cgtaccaaaa acagtatttg tccctcagcc aaatgtagat
    15721 tcagcgattt tgaaactgac aagaagagat ataccggcag tcgaagtgac agacgaaaaa
    15781 gcatttttcc gattgacaaa agcggcattt cagcaaagaa gaaaaacgct ttggaataac
    15841 ttgcagcatt cgtatggcaa agatgaccag acaaaagcat ggttagcaaa aagcttggaa
    15901 acagctggaa tcgatccaaa acgtcgtggg gagactttgt cattacaaga attcgccgca
    15961 ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga
    16021 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatgttttt
    16081 cgtttgtttg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtt
    16141 gtgggcctgc cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt
    16201 ttttgagtta gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa
    16261 ggagatgtga cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt
    16321 atacttgata ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt
    16381 attagtaagt gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa
    16441 aaaatgagaa aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg
    16501 gtcattatcc caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg
    16561 tctcatgtgc cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg
    16621 ctaggtacga catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg
    16681 tcacatcttg tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt
    16741 ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc
    16801 ggtgtgattc attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat
    16861 atgcctgaaa catattattc tcaaggatat ttttatacag tattcatctt gatggggatt
    16921 ggtgggctta tccacagttt agttgattat aatattgctt attttatagc aggaacactc
    16981 agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg
    17041 attcaagggt acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc
    17101 atctagtaat ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca
    17161 ccaacatctt ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc
    17221 tgtttcttta tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc
    17281 tggtttatgg aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt
    17341 gtaaagcatc gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc
    17401 ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa
    17461 gataacttct tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat
    17521 acgaagctta agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa
    17581 gttcatggca ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac
    17641 ttctttcggt gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc
    17701 catagtcatt gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg
    17761 gtgaagtttg gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa
    17821 tggctcatcc attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg
    17881 ttggcgttgt ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa
    17941 gatctctgct gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt
    18001 caagccaaaa gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa
    18061 aaccatggca atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt
    18121 tagttctcct tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc
    18181 acaaccggaa ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc
    18241 agttacagaa taattttctg cattgtcata ttttttatat acgtttttca gagccatttc
    18301 taccataata cgttccacac tttctttttt agttttatta actatttaaa gtataaatga
    18361 aagcgttaac taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt
    18421 gctagtttta agctttaata aatatccctg aataattcat agctttaatt ctttggcaca
    18481 ttttattgaa atatgtatca ctaatatccc tatcaactga ttttgactaa aaagttactt
    18541 caaacattca ctgttcaaaa atattgagga ataaattttg ggtgtaaggc attttgagca
    18601 tacttctccc tggataactt cccagtaaaa aaatcgttag attgttggat tagatccatt
    18661 gactggctcg cctcaggcga gcaaagaccc tgtagaattc attctgatac acatgataac
    18721 tagataattt gagatagact cgtctacccg tttggactaa tttcccagca actttcagaa
    18781 acttcaatcg aatagaatga atggtcattc ccttttggac ttcttcaaag ccaatcgttc
    18841 ttaagaagtt gactaagttg taagctatca agctgagcat cattcggaca tgattctcca
    18901 aaaagcgagg actgtctgtt ttgtcaaagt aaaagccagc tttcgcttct ttaatgaagt
    18961 tctccattgt gccacgtttg gcatagatag aaaagatggt atcaggagaa acattttctg
    19021 atagattcgt cacgataaat tcatgtcgaa agagtagttc gcccgcttca cgtgttgagc
    19081 gtatacacac gcgacgactt tgtgaccagg attgtgcttg ataagtggtc gagaagtact
    19141 gaacttctcg ttcttcccac ttttgattat cgccataaag tactgatttc tcagctattt
    19201 gacctagtct acgattattc ttcagtcgaa taacataatg actctttttt gattcacacg
    19261 aatcatacac accaggtgta gcgaacccgc tgtctccacg aaccaagatg tcagtgtttg
    19321 gtagagagtg attatagtgc tctaataaag gtgtgagaaa ctcctttacg ccttttgatg
    19381 tatattgatt tcctgaacgt agttcagctt ttaagaagtc accagtcaat ccgtcaaaag
    19441 ctaccaatgg atggtaacca taggtttggt agtgtgtatt ataattcgtt tgttcttggt
    19501 gaccaaatgt atctgaatgg gtcgaatcta aatcaatgat taattccgta tcattacgga
    19561 tgaggcgtgc tttatcgata agttcttggt tcaaagcttg aagttcatgg atattctcct
    19621 cagatagtcg atctaaaaat cgggaaagtg aagattgaga agcgagttct ttcctatcta
    19681 aaacagcttt aaacacagga tcttgtctca ggagattagc tgcagaatca gctgagtaac
    19741 cagcaattaa ttgcataatg aactgctcaa gtatggataa gttgtcatga gtaaaatacg
    19801 ctcgttcgtc ttcaatgtga atgtgttgct tagccagttc agaaaaacca aagatgttca
    19861 tcagctcttt cactaggacg agacccgaat cactcgataa ttgaccacct gtatgagaaa
    19921 tagtgatatt tgaattgaat tttacttggt ttttgtgtaa gctagtcatg aaaagaactc
    19981 ctttcttttg gttggtttag tcactttaac catagcagaa aggggttctt ttttcatcac
    20041 ttaacgggtg aagatgaaaa agtaattgta agctgccgtg aggcagtttc acatggtttt
    20101 aattaaaagt atgaattatt caggaatatc cataagaaat aaaaactgat acactggagt
    20161 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    20221 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    20281 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    20341 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    20401 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    20461 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    20521 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    20581 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    20641 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    20701 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    20761 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    20821 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    20881 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    20941 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    21001 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    21061 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    21121 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    21181 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    21241 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    21301 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    21361 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    21421 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    21481 aaaa
//
LOCUS       DAYYGY010000054        20254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000054 DAYYGY010000000
VERSION     DAYYGY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.055"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..1044
                     /locus_tag="ACHA52_001879"
     CDS             97..1044
                     /locus_tag="ACHA52_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940572.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1056..1976
                     /locus_tag="ACHA52_001880"
     CDS             1056..1976
                     /locus_tag="ACHA52_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8940573.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2017..3474
                     /locus_tag="ACHA52_001881"
     CDS             2017..3474
                     /locus_tag="ACHA52_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8940574.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3819..4433
                     /locus_tag="ACHA52_001882"
     CDS             3819..4433
                     /locus_tag="ACHA52_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8940575.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4426..6150
                     /locus_tag="ACHA52_001883"
     CDS             4426..6150
                     /locus_tag="ACHA52_001883"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8940576.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6147..7598
                     /locus_tag="ACHA52_001884"
     CDS             6147..7598
                     /locus_tag="ACHA52_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8940577.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(8071..10224)
                     /locus_tag="ACHA52_001885"
     CDS             complement(8071..10224)
                     /locus_tag="ACHA52_001885"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8940578.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKNKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(10226..11272)
                     /locus_tag="ACHA52_001886"
     CDS             complement(10226..11272)
                     /locus_tag="ACHA52_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8940579.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDFMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11422..12708
                     /locus_tag="ACHA52_001887"
     CDS             11422..12708
                     /locus_tag="ACHA52_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8940580.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            12724..15426
                     /locus_tag="ACHA52_001888"
     CDS             12724..15426
                     /locus_tag="ACHA52_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8940581.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15411..16268
                     /locus_tag="ACHA52_001889"
     CDS             15411..16268
                     /locus_tag="ACHA52_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8940582.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(16339..17196)
                     /locus_tag="ACHA52_001890"
     CDS             complement(16339..17196)
                     /locus_tag="ACHA52_001890"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8940583.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17407..19578
                     /locus_tag="ACHA52_001891"
     CDS             17407..19578
                     /locus_tag="ACHA52_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8940584.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19646..19987
                     /locus_tag="ACHA52_001892"
     CDS             19646..19987
                     /locus_tag="ACHA52_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8940585.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT         6753 a         3574 c         4172 g         5755 t
ORIGIN      
        1 ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta
       61 taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat
      121 cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg
      181 ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat
      241 tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg
      301 gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt
      361 ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt
      421 atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct
      481 tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa
      541 ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa
      601 ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac
      661 agcattattt actttgcatc tgttatggga atcgatccga cttattatga agctgctatg
      721 gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca
      781 ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg
      841 ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg
      901 tatatctaca atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt
      961 taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt
     1021 gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag
     1081 tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg attgcactct
     1141 ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt
     1201 ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata
     1261 cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg
     1321 ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt
     1381 cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt
     1441 tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata
     1501 cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca
     1561 cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta
     1621 gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa
     1681 tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc
     1741 aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat
     1801 atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag
     1861 gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc
     1921 catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa
     1981 acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc
     2041 tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct
     2101 aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa
     2161 ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt
     2221 gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc
     2281 atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa
     2341 aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat
     2401 gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca
     2461 gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa
     2521 tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa
     2581 ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa
     2641 gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat
     2701 gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg
     2761 catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat
     2821 ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc
     2881 gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca
     2941 ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat
     3001 aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg
     3061 cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc
     3121 aaattgctag atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac
     3181 aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg
     3241 attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa
     3301 acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg
     3361 actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat
     3421 gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga
     3481 ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt
     3541 gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat
     3601 tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg
     3661 gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt
     3721 tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat
     3781 aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag gttttggaag
     3841 agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat
     3901 ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt
     3961 atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa
     4021 gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact gctttattga
     4081 gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct attgatttta
     4141 ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata
     4201 gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca
     4261 gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc
     4321 aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga
     4381 tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg
     4441 attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg
     4501 atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa
     4561 aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat
     4621 aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa
     4681 aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag
     4741 caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat
     4801 tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct
     4861 tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt
     4921 tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt
     4981 caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta
     5041 tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa
     5101 gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg
     5161 ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat
     5221 cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc
     5281 ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca
     5341 ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca
     5401 gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca
     5461 atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta
     5521 gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt
     5581 ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta
     5641 gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg
     5701 atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt
     5761 tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta
     5821 cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat tgattttact
     5881 cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc
     5941 atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg
     6001 tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta
     6061 cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg
     6121 accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg
     6181 aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa
     6241 tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg
     6301 atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg
     6361 ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt
     6421 atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt
     6481 tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa
     6541 aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg
     6601 atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat
     6661 tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac
     6721 aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg
     6781 cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc
     6841 tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag
     6901 cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat gttttttacg
     6961 cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc
     7021 aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt
     7081 tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt atggagattc
     7141 aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc
     7201 aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga
     7261 gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc
     7321 aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag
     7381 gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta
     7441 tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg
     7501 agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat
     7561 cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa
     7621 ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa
     7681 ggcagctttt tcatgacctc tcgataaacg gagttcaaaa gccagtttct cggtattaag
     7741 ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac
     7801 taaacagctt tttcacgacc tctccactaa acggtgttca atcagctgac cctcggtatt
     7861 agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg
     7921 tcagaacgct gatttcacaa cctctcaata aacggtgttc aaaagcttgc atctgcggta
     7981 ataagaccaa ccaaacgaaa aatatgagga tctatttttg cttggctgtt cttattactt
     8041 gatgcaggac agctttttca caacctcttg ttaattatag tttgataagg aaaaaggcaa
     8101 atcttttgct tgatatttct tagggtgagg gatgatccct aattggtaag agatcgtgcc
     8161 gcctttcagc aaatcttgat gggtaaaata taatttcgta tattctttgt ccatccgttt
     8221 gattttatgg ataaattgtt gctgcggccg attaggcgtt gcttcaatcg tcaattcctt
     8281 tccatttgac aaaagcaaaa cagccttgtc aattaatggc ataccgataa catattcacc
     8341 ggttccaggc gtgacaggat aaaatcccag actattgaag atataccaag cggccatact
     8401 tccattgtcc tcatctccag ggtaacccat tggagaatcg tcgaaggtct gagtcatcaa
     8461 ctgtttaagc agtggctgtg ccatttcagg ttgtcctata taactgaaca agtaaggata
     8521 gtgaaaactt ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg ccatctcact
     8581 catttcgtgg atctcaaaac catatcctcc tgggtcaaac gttgggggct gattgcacag
     8641 ctcaatgagt ttttctttat atctctcact gccaccatat tttccgatca gtcctgaaaa
     8701 atcgtgataa acagcaaagc tgctttgcca agcacttcct tctgcataat cttgtcccca
     8761 acgattagct gaaaatggtt ctctgaactt gccatcttta ttttttgaac gcataaatcc
     8821 tgactcatag tcaaagatat tttgatagtt caatgattgc tgctgatatt tcttcgtcat
     8881 ttcctcttct tctagtacct tggcaacttg gctaatacaa aaatcgctat aagcatagtc
     8941 taatgtgtga ttgactgatt cgtggtagtg attaggaaca tagccatatg tcaagtaatc
     9001 ttctgttcct tggcgtccat aatttggatg tttgctttga accgtagcac ctttttccat
     9061 tgccgttaga aattcaggca tcaagtctcg tcggatatct tttacagcag catctgcaat
     9121 gaccgcatcg atcaatgtcc caggcataag tccacgttca tcaggagaca gccattttgg
     9181 caaatagcca ctttctctat agctgttcaa aaaaccttcc agcatttctt cgtatttttc
     9241 tacagcaatc aaagaataaa gaggataaac cgttttatac gtatcccaaa acccattatt
     9301 tgtatagaga acaccaggtt ttatagatcg actagtcgta tcatagtgga ttttttgccc
     9361 actaggatca atttcataaa atgtttgtgg aaataaaaac acccgatata aattatgata
     9421 aaacgtccgc aattgttgtt gatcatggtg tgtgagctgt atacgatcaa aataggactg
     9481 ccattgttct tgactgtgag caaggtaagt ctctttgctt tggttttgtt ctagttccag
     9541 gttataagct gcttgttccg agctgataaa agatgtccca aatcggatag tttgtttttt
     9601 tgtctgttca aaatacaacg ggatcgagtc gctatttttg ttggaaacag ctaactcatc
     9661 aattgttaaa ggatattcaa atctcaatat aaaataaaaa gaaaaatcag gatcttctga
     9721 accagcaaaa tgtgtgattt ttccttccac tgtgtaagga tctataacaa agagctgatg
     9781 gcgtcccgga aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa tagataagat
     9841 tccgccatac atgctaggta tcaatgtaga acagatgcca taacgtaatt gacagatcga
     9901 cagatgagtc gggcaaaata tactttcttc tgggcggtaa gaactttgag catggaaaag
     9961 ggtgttctcc tgtagttttc cattgatcgg cgtaaacagc atatgactga aatcacccat
    10021 ccaaggactt ggttgatggg taagacggta gccttgaaaa acgcggtcat caggatgaaa
    10081 ccaccagctg ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc caaaaggaac
    10141 gccagtataa ggctggcagt tcccattaga gaaatttggc tgattcgctg ttgcatgtct
    10201 agtatctatt tgaccgatat tcattttatt tctcctttgt tgattgtctt ttgatgaatg
    10261 aaactggaaa tttactatcg gatggtcgat cttttgtctc taaccaagtg accaaagcat
    10321 ctcccgcaag cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt ggcgggtcaa
    10381 caagagctgc tgcttggata tcatcaaatc cgataattga aaaatcttca gggatatgat
    10441 aaccatgctg tctcaagaga ttcatggtct ggatagcgac aacatcgttt tcacaaacaa
    10501 aagcagttac ttcatactta gtgatataag aaagcaattc atctaatgtc gcgcttttgt
    10561 catccagtga agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg taaccaaggt
    10621 aacgctgtcg aacggattgt ggatgagtct gcgtaccaaa caaataagcg atttttgaat
    10681 gtccctgttc gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta tcggaaagaa
    10741 cggtaggaaa aggtaactca tagatttttt tatctaatat gacgacaggg aattctttca
    10801 atgaaagctc aaaaagtaag tcaagatgtt gttctgtatc aagagcataa taaatcaatc
    10861 cgtcaaattc attgattagc tcttttgctg ttttattttg tagaaagtca agcgtcgtca
    10921 tcatgaaatc aatctgttta tcttgcatca cagggttcaa tcccttgcta aaatcaccaa
    10981 cagacaaatc agcgacatat ggaagaagaa acagaatccg tcgtgttttt gtaggagaag
    11041 caagaatctg ttccggagaa gcggatttaa cgaaactgcc tttcccgcga acgcgataaa
    11101 taagtcctaa ttgctctaat tcagtcaatg cacgtttgga agtgattctg cttactttat
    11161 aggtacgtga aagttctttt tcagttggta gttggctgtc tacaggtaat tcaccagtta
    11221 aaatagccgt ttgcaaatca ttaaatattg tctggtacaa tggttgattc atataaacac
    11281 ctcttaatga tatatcatat tcaaataata ccataatgga tgaaaaagta aataaaaaga
    11341 tattttttga aagcgtagac ataattggct gtatgaagta taatctttaa agatatatca
    11401 aatagaaaga aggttttatt agtggcttat aaagatatac caaaatcggt gcagaaattt
    11461 atggaagata tcacacaaaa atgtggggat atccatgcgg actgggcaaa gaattttaca
    11521 gcagcatttg ccaatactct gttgacaaca gtcaaaaagc aaaatgacgg aaccaccttt
    11581 ttattgacgg gagacattcc agcgatgtgg ttaagagact ctactgcaca agttcgtcct
    11641 tatttagtga ttgcaaaaga agatgaggaa ttagctgaca tgatcagcgg gttagtcaaa
    11701 aagcaatttt tttatatcaa tatcgatcca tatgcgaatg ccttcaacga agaagctaac
    11761 ggtgctggcc accaaacaga tcatacaaaa atgaacgatt ggatctggga aagaaaatat
    11821 gaaattgatt ctttatgcta cccaatacag ttggcctatc ttctttacaa aaatacagga
    11881 aaaactgatc aattcaatca atcttttatt gaaggtatca aaaaaatctt ggacgtgttc
    11941 aaaacagagc aagatcatga acactctcct tatacatttg tacgagacac gacaagaaaa
    12001 gaagatacgc tgatcaatga cgggcgaggt gcaaaaacag cttttacagg aatgacatgg
    12061 tctggtttcc gtcctagcga tgatacttgc cgttatggat atctggtacc atccaatatg
    12121 tttgcggtgg tcgtgttagg ttatgtggaa gaaatttttc aaacgatact cgatgataaa
    12181 gaaattgtac agcaaacaca gaaattgaag aaagagatcc aagaggggat tcagaatcac
    12241 ggacgaacgg ttaatcaaaa agggcaaacg atttatgcat atgaagtaga tggaatggga
    12301 aacgccacag tcatggatga cagtaatgtg cccaatctga tcgccgcacc ttacttggga
    12361 tattgttctg aacaagagga agattatctg caaacaagaa gaacattgtt gagtaaagaa
    12421 aatccatatt tctatgaagg aaagtacgca aaaggaattg gatcctccca tacgccagaa
    12481 aaatacgttt ggccgattgc attagctatg gaaggaatga caacgtcaga caaatctgaa
    12541 aaagagcgga tattgaattt acttgtagca actgacgcag gaacacacct tatgcacgaa
    12601 ggattcgatg tgaacaatcc tgaaaattat acgagagaat ggttttcttg ggcaaatatg
    12661 atgttttgcg aattagtcat ggattacttt gatattagag tagaatagta ggaggtatcg
    12721 taaatgaaaa agaaagtata tatcatttct catagccact gggatcgtga atggtacatg
    12781 gcttacgaac agcatcacat gcgactgatt gaattgatag acgatctact agagctattt
    12841 gaagttgatc cgtccttcaa cagttttcat ttggatgggc aaacgattat tttagatgac
    12901 tatcttcaag taaggccaga aaaaagacag gcaatccaac aagcaatcaa tgaaggaaaa
    12961 ttgagaattg gaccatttta tatcttgcaa gatgattttc ttatcagctc ggaatcgaac
    13021 gtgagaaata tgctgattgg catggaagaa agccgtaaat ggggaacccc tgtcatgtta
    13081 ggctattttc ccgatacatt tggaaatatg ggacagacgc ctcaattaat gaaacaagct
    13141 ggtatttcag cagcagcgtt tggccgtggt gtaaaaccaa ttggctttga taatcaagtt
    13201 ttggaagcag aaaactattc ttcccaatac tcagagatgt ggtggaaagg acctgatcaa
    13261 acagcaatct ttggtctgct atttgccaat tggtatagca acgggaatga gattccagta
    13321 gaaaaagaag cagctcttgc cttttggaag caaaaattag cggatgcaga acaatatgcc
    13381 tctacgaacc acttattgat gatgaatggt gttgatcatc aaccggtaca aaaagatata
    13441 tcaaaagcaa tccatctcgc aaatgaactg tttccagatt acgagtttat tcattctaat
    13501 tttactgact atctggaagc agttcaaaaa gatgtgccag aagacttagg ctcagtagaa
    13561 ggagaattga caagtcaaga aacggatggc tggtatactt tagccaatac agcatctgct
    13621 cgggtttact taaaacaatg gaataccaag gtccagcgac agctagaaaa tattacagaa
    13681 ccacttgcga cgatggctta tgaagttagt ggaaactatc cccatgatca attagattat
    13741 gcttggaaga cattaatgca aaatcatcct cacgacagta tttgcggttg ttcagtcgat
    13801 tctgtccatc gggaaatgat acctcgtttt gaaaaagcag atgaagttgg aaagtatctt
    13861 gcacaagatt ccttagaaca gctgaccgca gcaatcgata cgaccggatt cccaaaagac
    13921 agctttccat ttgtcattgt aaatactgca ggaatggata aaactggtga agcagaaata
    13981 accatcgaat tagaaagaaa aagattcgct gaaggaatac ctgaacaact atatcaagag
    14041 ttagaaaatt tgcctaaacg taagtatcat gtagaaacaa aaagtggggc cactatacca
    14101 gctattttat ctgaagaaac tgtccaattt ggttatgatt tgccgaaaga ccgtttccgt
    14161 gtgccatata tggcaagaat gatcaaagtg actttaccat tagaaaacat gccagcattt
    14221 tcttgggaaa cattcgcgct tgtagaaggg gaagcgaaag cagaagaaaa ggaaacaatg
    14281 attcatcaga gtggacgaat catcgaaaac ggccctctac atttgacgat tgaaaaaaat
    14341 ggtaccatta caatggaaga tcgcaaaaac aaacgaaagt tgaataatct gcatattttt
    14401 gaagatatcg gcgatatagg aaatgagtat atctttaaac aacctttctg tgatcagcca
    14461 atcctatcca gtaacaaaga aaactctgaa gtaaaagtat tagtagatac accggaaata
    14521 gcaaaaataa gtattctaca ggaaatggaa atccctgttt ctgcagacga gcgtttagaa
    14581 aaagaacagc agatggttgt ggaatttcgt tatcgaaaag cagaacgatc caaagaaaaa
    14641 cgaatccttc aaataaaaac aatcatgaca atcagaaaag atagtaaaaa aatcgacttt
    14701 gaaacgactc tcgacaatca gatgaaagat catcgcttac gtgtcttgtt tcctacaaag
    14761 ttacatgtgg aacatcacga agcagatagt atttttgaag tggtgaagcg cccaaaccat
    14821 gtgtctaaaa gttgggaaaa ccccacgaat ccgcaacatc agcaagcatt tgtcaatatt
    14881 catgatgaag agtacggtgt aacagtcgga aactttggtt tgaatgaata tgaagtaaca
    14941 gaagacggac aaatcgcagt gacgctactt cgcagtgtgg gagaactagg cgattgggga
    15001 tattttccaa caccagaagc acagtgtcta ggtgagcatc ggttcaatta cagcattgaa
    15061 ctacatggac cagaagaaaa attctctact tatttgcatg catacgcagc acagattcct
    15121 ttttccactc aacaaatcaa acatcatgaa ggaacattga tttcaaaaca acaatattta
    15181 acgataaaaa gtgaaacgtt tgcaattaca gctttgaaac gcagcaagtt tagtgataaa
    15241 gtagtagtaa gaggtttcaa tatgtctagt catttagaaa aattggaaat aactaaagac
    15301 aatggaaaaa ctgtcatatt gaatctgctt gaagaaccaa cgaagcaagc ggttgtccca
    15361 ataatccaac cttatgagat tcggacaatc ggattcgagg aggaaaacga atgtatggcg
    15421 gaatagaagc aggcggaaca aaatttgtgt gtgcggtttc agatggtgga gaaattttag
    15481 aaaaattaag tatccctacc acaacaccag aagaaacatt ggatcaggta tttgaatttt
    15541 ttgatcgtta tgaattgaag ggaatcggga ttggctcatt cggcccgatt ggtatcgaca
    15601 aaacaaaaga taattacgga tacgttttgg caactcccaa aaaagggtgg gcacaattcg
    15661 attttcttgg cacaatcaag gaacgctaca atattcctgc tgcttggaca acggatgtca
    15721 atgctgctgc atatggggaa ttgaagcaag gagcagcaca aggaaaaaat agttgtattt
    15781 accttaccgt gggaacagga atcggtgcag gtgtcgtgat caatgaagaa attttttcag
    15841 ggatcgcaca tcctgaaatg ggacatattt gggtgaaacg ccatccagat gaccattatg
    15901 aaggtacttg tccttatcat aaagattgtt tagaaggatt agcagcagga ccgtctattg
    15961 aggcacgcac agggatcaaa gggcaagatc tgccgcaaga tcatcctgtt tgggctattc
    16021 aggcttatta tattgctcaa gcactgatca actatacgtt gacgttagca cctgaaaaga
    16081 ttattttagg cggtggggta atgaatcagg atcatctcct tcaaaaaatc cgtcagcaat
    16141 ttgttgaatt gatgggtggt tatatggaaa cacctcaagt aagtgaatat atcgttcgtt
    16201 ggggactgcc gaatgaaagt gggattatcg gtagtttgct gttagcagaa cacgaacatc
    16261 aaaagtaagc agacaaatta cacaaaaaga acagagtctt atcattatct gctgataaga
    16321 ctctgttctt ttttatttct attcccataa tccaggtaaa gctttaccga ctattttttc
    16381 ttgagtaaat ggatggatca tgtgcagttc taatgcatgg agcatcagtc gttcagctgt
    16441 tctattttgg tagagagggt ctccaacgat cggatgtccc ttatgagcta aatgaactct
    16501 gatctggtgt gtacgtccgg tttccaaaac acaatagact tgtgtttgtt cagaaagtac
    16561 tttgttgaca gtgacatgtg tcaaggcgct ctgaccattt ttatcatcta cgaccctttt
    16621 tctccgatca tggcgatctc gtccaatttt attggtgatg gtcatatctt tggcaatttc
    16681 tccccatacg atggcttgat atctccgata gatcattttt tgttccaaca ttcttccaag
    16741 tatcggcaag acaaatggat tttttgcaaa taaaacggca ccgcttgttt ctttgtccaa
    16801 gcggtggaca acgtaaggtc gttgctgttt agtgtcgaga taagcagcta aatgattgag
    16861 taaggtactg ttttctgtcg gttcatttgg atgagtcttc attcctgcag gcttatccac
    16921 tataatcaaa tgctcatctt catataaaac acggatgtgc ttcgcatctc ctaattgaac
    16981 ttcttgataa ttataatctg tttcttcgaa taacaatgtg atctggtcat tgccctttac
    17041 ttcatgatgg aacaaagcag gttgttgatt gatccaaacg tttttacgta tacgcaaaaa
    17101 gtgtcgaacc tttctaggaa ctaaccattc cttttcaagc aagtcgcgaa ttgttgtttc
    17161 aggatggttc ataggtaaag tgatcgtgat tttcacaagt gtactccttt cagtgcaatc
    17221 gcagactaat tgtaccattt ttaaaacata tttaagaaaa aacttacatt tttctgttca
    17281 ttcttatgtt actctacttg cactaacgta aaaaaacata ttgaactaca acttaagcag
    17341 gaagaaaaca ctctttcact atggtaaaat aagacgattg aatcaaatag acaggagaag
    17401 aagcaaatgg attttcaaag cattttagaa aagataaaag ctgctttgat cagtttttgg
    17461 cgttggatca gaccatatct cggacaattc catcgatggc gaaagcgcat ctggaaaaaa
    17521 tatcatgtga acaaattgat tttgctgatt ggtttgatct gtgtcttagt gacaagtatc
    17581 tacctcttta ttttagcaaa acaagcaaat gtcgaaacgt taaaatcagg attaagccaa
    17641 tcgacagtca tttatgataa gaaaaacgaa gaagctggca cactgtacgc ccaaaaagga
    17701 acatatgtag aattaaacgc gatttctccc tatatccaag atgcagtaat ctcgacagaa
    17761 gataggaatt tttacgagca tcacgggttt gatatcaaag ggattgcccg tgcagcagtc
    17821 cgaatggtct tgtctggcgg aacatcaggg ggcggaggaa gtacgatcac tcagcagctt
    17881 gccaaaaatg cgtatctgac attagaccaa acgtttgatc gaaaagcgaa ggaattattt
    17941 ttggcaatcg agatcgaaaa gaagtacagc aaagaagaaa tcttgaccat gtatcttaac
    18001 aatgcctatt ttggcaacgg ggtatggggt gtccaagacg cttccagaaa gtattttgga
    18061 gtagatgcaa cgaatgttac gctgtctgag gctgctactt tggcaggaat gctgaaaggt
    18121 cctggtattt ataatccgat cgatcaccca aaaaatgcga atgatcggaa aaacacagta
    18181 ctaagtgtaa tggaagaaaa cggaaaaatc acaaaagaac aagaacaagc agaaagccag
    18241 acagatatca gcgcgtatct taatgacaca tacgataatt cagattctgg ttatcgttat
    18301 ccttattatt ttgatgcagt gatcgatgaa gcagttaacg agtatggtct tgatgaagaa
    18361 gatgtcatga ataaaggcta taaaatctat acagctttag atcaggatta tcaaaagcaa
    18421 ttagaagcta cttatcaaaa cagtgcatta ttcccagcaa atgcttcaga tggtgccatg
    18481 gtccaatctg cttcagtagc tttagatccg aatacaggtg gtgtacaagc actagtagga
    18541 cgaagaggag atcatgtctt tagaggattc agctttgcta cacagatgcg tcgttcacca
    18601 ggatcaacga tcaaaccatt aagcgtatat acaccagcac tggaagctgg atacaagcct
    18661 tccagtatct tgaaagacga accgcaaagt tattacgatg cgcataattt tgacgggaca
    18721 taccaaggag aagtaccgat gtatcaagct gttgcacaaa gtttgaattt gcctactgta
    18781 tggcttttgc atgagatcgg tttacaaaaa ggatatgata aagcagaaga attcggcctg
    18841 cctttagcaa aatctgataa gtattatggc ttagcccttg gtggactgga aaaaggagta
    18901 tctccattga tcatggcggg tgcttacagt gcatttgcca atgatggtca gatgtatacg
    18961 ccgcatctta tcacgaaaat cgttgattcg actggagcgg tgatcgtcga taacacaaac
    19021 aaaaaaccaa aacaggtcat atcaaaggaa acagccgatg aaatgaccag catgcttctt
    19081 ggtacatttt caaatggaac agcggctgct gcgaatcctg caggttatac gatagcaggg
    19141 aaaacaggaa ccacagaaac taatttcgat gccacaaaag caaatgacca atggatgatc
    19201 ggttacacgc cagacgtagt gatttcgaca tggatgggtt acgaagaatc cagcaagctg
    19261 cactatttgg aagggacaag tggaaatgtt gtcgggaaag tcttcaaatc agcggcagaa
    19321 ggcatccttc cgtatacttc aaaaacagga tttagtgtag cagatgccta tgccacagga
    19381 ggacaagtgg tagctgcaga tcaagttgga aacacaggaa attctaatgg agagtctgga
    19441 aattggcaag ataacttgaa tcaatatggt gaacaagcaa aagaagggct gaaaaacttc
    19501 ggcgatatgg tgaaagaagg cgttcaggga attggtgaag cggctaaaga cctctggcgg
    19561 aaatatcaag gagagtagcc cctttgaaaa cgaataaccc catgttacaa tagagagtaa
    19621 ctactaaaag aaaagagggc ataacatgtc aaacatctac gatagcgcga atcaaatcga
    19681 acgtgagatc cgtgaattac cagaattcaa agcgttgaaa gaagcgtacg agaaagtaca
    19741 agcaaacgaa gaagcacaca aactgttcaa agacttccaa gcgatgcaaa tcgaattaca
    19801 agaaaaacaa atgagtggac aagagttcag cgacgaagat gcaatgaaag cacaagaaat
    19861 ggctgtgaaa atccaatcag aagaagtaat caatgacttg atgcaaaaag agcaaggctt
    19921 cagtacaatc atcaatgact tgaaccgtat catcatgaca ccagttcgcg acctttacag
    19981 cgaataaaac tattttatga aacaacagag ggtgtgaaag agacgttcgg ccttgagcga
    20041 tgacaaaatt gcttcttatt gccgataggc tgtctcttga acaccattta ccaagaggtc
    20101 gtgagcctac cgtttaactc tgagttacaa ggaacaattt caaaaaacag cttctcatgt
    20161 ttgatgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcagaaa
    20221 taggagctac gacaaaagtc ttgtcatagc tcct
//
LOCUS       DAYYGY010000055        20236 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000055 DAYYGY010000000
VERSION     DAYYGY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.056"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1248)
                     /locus_tag="ACHA52_001893"
     CDS             complement(64..1248)
                     /locus_tag="ACHA52_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8940586.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1273..2280)
                     /locus_tag="ACHA52_001894"
     CDS             complement(1273..2280)
                     /locus_tag="ACHA52_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8940587.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2452..2811)
                     /gene="comGG"
                     /locus_tag="ACHA52_001895"
     CDS             complement(2452..2811)
                     /gene="comGG"
                     /locus_tag="ACHA52_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8940588.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2783..3208)
                     /gene="comGF"
                     /locus_tag="ACHA52_001896"
     CDS             complement(2783..3208)
                     /gene="comGF"
                     /locus_tag="ACHA52_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8940589.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3171..3518)
                     /locus_tag="ACHA52_001897"
     CDS             complement(3171..3518)
                     /locus_tag="ACHA52_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940590.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3490..3960)
                     /gene="comGD"
                     /locus_tag="ACHA52_001898"
     CDS             complement(3490..3960)
                     /gene="comGD"
                     /locus_tag="ACHA52_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8940591.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3935..4213)
                     /gene="comGC"
                     /locus_tag="ACHA52_001899"
     CDS             complement(3935..4213)
                     /gene="comGC"
                     /locus_tag="ACHA52_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8940592.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4210..5259)
                     /gene="comGB"
                     /locus_tag="ACHA52_001900"
     CDS             complement(4210..5259)
                     /gene="comGB"
                     /locus_tag="ACHA52_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8940593.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5216..6145)
                     /gene="comGA"
                     /locus_tag="ACHA52_001901"
     CDS             complement(5216..6145)
                     /gene="comGA"
                     /locus_tag="ACHA52_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8940594.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6277..8031
                     /gene="ade"
                     /locus_tag="ACHA52_001902"
     CDS             6277..8031
                     /gene="ade"
                     /locus_tag="ACHA52_001902"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8940595.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAHPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8129..8791)
                     /gene="trmB"
                     /locus_tag="ACHA52_001903"
     CDS             complement(8129..8791)
                     /gene="trmB"
                     /locus_tag="ACHA52_001903"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8940596.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8861..9643)
                     /locus_tag="ACHA52_001904"
     CDS             complement(8861..9643)
                     /locus_tag="ACHA52_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8940597.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9847..11058)
                     /locus_tag="ACHA52_001905"
     CDS             complement(9847..11058)
                     /locus_tag="ACHA52_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940598.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11051..11788)
                     /locus_tag="ACHA52_001906"
     CDS             complement(11051..11788)
                     /locus_tag="ACHA52_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940599.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11926..12360
                     /locus_tag="ACHA52_001907"
     CDS             11926..12360
                     /locus_tag="ACHA52_001907"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8940600.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12353..12697
                     /locus_tag="ACHA52_001908"
     CDS             12353..12697
                     /locus_tag="ACHA52_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8940601.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12877..13887
                     /locus_tag="ACHA52_001909"
     CDS             12877..13887
                     /locus_tag="ACHA52_001909"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8940602.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(13956..14900)
                     /locus_tag="ACHA52_001910"
     CDS             complement(13956..14900)
                     /locus_tag="ACHA52_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8940603.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(14884..17604)
                     /locus_tag="ACHA52_001911"
     CDS             complement(14884..17604)
                     /locus_tag="ACHA52_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8940604.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(17601..18821)
                     /locus_tag="ACHA52_001912"
     CDS             complement(17601..18821)
                     /locus_tag="ACHA52_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8940605.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(19102..19947)
                     /locus_tag="ACHA52_001913"
     CDS             complement(19102..19947)
                     /locus_tag="ACHA52_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8940606.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         6182 a         4072 c         3326 g         6656 t
ORIGIN      
        1 aaaacacatg caagaattcc ctcttgcatg cgtttcatct tttatcctaa cgaatgcttt
       61 ggcttagccg cgcagacgct ccacatcacg ggcaatcatt agttcctcat ctgttgggat
      121 cactaatact cttacttttg attcttttgt agagatatct acttcttcac cacgcaattc
      181 attcaacgct ggatcgattt cacaaccaaa ccaagacata cctttgatga tttcactacg
      241 tacatgtccg tcattctcgc cgataccagc agtaaagaca attgcatcaa caccattcat
      301 cgttgtcaca taaccgccga tgtattttcg gatgcggtcc acgaaaatgt cataagctaa
      361 acgaatatgt tctttttcat agttcttttc aagatcacgc atatcactag acaatcctgt
      421 taggcctagc agaccagatt tcttgttcaa aacattgatc atttcatcaa tatcaatctc
      481 taatttttcc attaaatatg ggagaacagc tggatcgata tcacctgaac gtgtacccat
      541 tgtcacccca gctaatggtg tgaatcccat agaagtatcc atggatttac ctccatcgac
      601 tgctgtgatc gatacaccat ttcctaagtg gcaagtgatg atttttagtt cttcgatcgg
      661 tttgttcaat aattttgccg cacgttccga cacataacga tgacttgtgc catggaagcc
      721 atattttctt actttgaaat cttcataata agacaccggt aagctatata aataattttg
      781 ctttggcatt gttgagtgga aagatgtatc gaatacagcc acactcaaaa tgtcaggtaa
      841 aatcttttta aacgctttga tccccattaa gtttgcagga ttatgcaaag gagcaaattc
      901 tgctaattca gcaatgcggt ccattacttt ctcatcgatc actactgaat ccccataagt
      961 ttctcctccg tgaacaacac ggtgccctac tccggtgatt tcatcataag agcttaaaat
     1021 ttgcagttca atcaactgat ctaacagcat ttttactgct acttcgtggt tttcgatatc
     1081 catgatttgt tcgaattttt gattatcgcc gtacttgatg gtaaaaatag aatcttgtaa
     1141 accgatacgt tcgacgatcc ctttcgcaac tacggtttct tctggcattt gatataattg
     1201 ccatttcaaa cttgaactcc cagcattgat tgcaattgtt ttagacatat gactctcctt
     1261 ctcgttgaat gattacagag cagatgattt ccagttttta aactcgttga aaaattccgt
     1321 caccttctct ggttctttta atgaagcaag tttcacaagc aagacttctt ttacttgttg
     1381 tgcttgtggt ccttttttct gtaagatcaa aatacttttt tgcgattgtt tattacggaa
     1441 caattcatct ggcaattgaa tcattccttg aagataacct tcttctttaa accatttttt
     1501 gatttcttca ctttgatccg tttctaaaaa tccactcggc atcaaaaaca aaccaaaacc
     1561 atccggcttc acatatttca ttgattgctc tagcaataaa tgatgtgcat aactatggcc
     1621 ttcttcggta cttgttaaaa actcttttgc tttttcgtca ttcggataat aaccgattgg
     1681 caaatcgctg attgcaaaat cgacaggatc tattaataat tcctgcaacc cgtcttgatg
     1741 aaagtattga acatttgctt gtgtcaaatc acttgtagaa gctgcgacag ccaacaatgt
     1801 atcatcaata tccacaccaa ttccttctgt ttggtacccc gcattcgata ggtttaaaag
     1861 aactgttaat aacaagttgc ccattcctgc cgcaatatcc agaatcttaa ccggtgcttt
     1921 tttatttgtg tatagttgtt caataagaaa aacaaataaa aaaccgatac tatcaggagt
     1981 gagttgatgg ttcgcctgca aatgttctgt ctggctgccc ttcaataaaa gtaattgaga
     2041 cagtctgcgc cattcttctg gctcgaaaga aagtttcttc aactcttcat aaagagctgt
     2101 gatccgttgc gttgtctctt ttgtcggcac tccatccacc acacgtactt gataatcatc
     2161 tattaaattt tctgcatttt cgacataggc ttctaaaaag gaagttccca atgcattctg
     2221 taaaagctga atagcttcca ggttttgatt aaacccttgt tccatttttt ctggtaacat
     2281 ggtgcacctc atttctagat atattataac aattttttca cctgtttaat tctatcgaac
     2341 aattgacaaa atttcaatcg ttttcactgt tttttatttg tgaaacggca aacatttttt
     2401 tattaaaaac agtgttataa cagaaaagag ggcgttttca cgaacattcc atcattctgt
     2461 actagtatct gtatcatttt ttacttcttc tgtgaaccga aagataaacg gtgagacttc
     2521 cacctctaag aatagggact gttgtaccca ctgatatgtt acagatccag ttgtgtagta
     2581 aaaaactcct tccgaacttt cggcaagcgt agactgttcc gataaagcca tctgcttcat
     2641 tatttttgct atataataat ttttgattcg tatattaaga tccattgaca aacggtagct
     2701 ttctaatgct gccatcaata aaaacgtaca taaaagaaat aaaagcaaga cagaaaataa
     2761 aatagagccc ttataacatt ttctactgat cgttttttgt ccatatggca tatccatctt
     2821 ctccattttc aaagtgtaca taaaaagaaa ttgtgtgttc tttttccaat aattgaagct
     2881 cagtgatact tgttaacata ggttgatgtc cgccggaagt tcttctttta cgtatcattg
     2941 cctgatatct ttcgatgata tactgtttat cctcgatcgt gtagtgcagt tgctgatttt
     3001 tcacagagac attgtgactg ctcttgagtt cttcttctaa ttgtgctaaa aatattagcc
     3061 actctttttg atctttacgc tgcaaaaatt cacttacttg gatggtttgc tgaatcaaaa
     3121 gattgaatgt gagcaataga ctgctcaata tcatcagtcc aattagacat tcaatcagcg
     3181 taaatgctgg aattacttgt tttttcaata ctgatcgctt ctttctccat attgacaaac
     3241 actttttcga ttactccatt attatcgaca atttggatat cgtatgggat catttcttcc
     3301 cttcgctctg gtcttttgtg atacatccgc tgcgtcttga tatcttcata aatcattcga
     3361 ataaatagca gttcctcccc acttttcttt tctgcctgaa gcaaaaacag ctggccaaag
     3421 ctcactaagt agacacagac agaagcgatc ataagcgctg taagggactc caacaaaaca
     3481 aatcctgttt tattcaattc tttttgtata gcgaccactc cctatctgga attgatacgt
     3541 aatcgtccgc tctttttctt cccaataaaa ttgataagct tttagtgaac tttcattccc
     3601 ggttctagca gcaaaagtaa tagaagcatg gcgtttcaag gtaatctcat caggaatatc
     3661 taatacttcc caatttattt tatctggatt cggaacatga aaaatgattt gattctcttc
     3721 actattccaa taaaagcctg tttggatctg attcacaata gcgatttttt gcgtagaata
     3781 gattcgtttt tcaaattggc cgaaaaattg atagactgcc aattcattct tccatgcaga
     3841 aaatgcgaga actggaaaag aaaggatcgt tgtaagtaat aaaatcagta agagtgattc
     3901 aaacaacgta tatcctgatg ctaaaacctt accttcaagg ccgctgatag atttctgcat
     3961 gttctcgttt gatatagcct tcattcaaca attcctccac agtaggaact cttccattat
     4021 tttgaagcgc atagagctct ttttgtgtgt ctaccaactg gatgatcgct tcacgacttt
     4081 cttggtctac atgattccta taacgcgaca aattagggac gaacaacaac actaatacac
     4141 tgatgatcag taagacgatt agcatttcga tcaatgtaaa accgctataa taagatattt
     4201 ttctcttttt catggcaaaa cctcctctat gcttccgtaa acaggtaata aaacagcggc
     4261 ataaactaat aaaatcaaag ccgctacgcc caaaaaaatg attggctgaa taaaatgcat
     4321 ccagtgatga agacgtttga aaagctgttt tcttgtccat tcgctgtaaa aaatcagttc
     4381 tttacctaaa cttccctttg cctctccctg taaaacaatt ttactgaatt cttctgatag
     4441 aaaacgataa ttctggaact gctcagccaa tgacatacct gcctccaatc cttgaatcaa
     4501 cttagaggca agatgctgaa tcagactttc ttgtttcgtt tcctttaagc agactatgat
     4561 ttgccttaat tcgaaacctt catgaaaaag ctttccaatc tctagtgaca aataagccga
     4621 ttgatacaag gaatatatcg gtccaatcaa taattgtctg cttatccatt cactctgatc
     4681 aacaggagac aacttcatca gacgatacct tactagtaaa gccaatagag taaccgctag
     4741 gatgaatagt agaacatacc aatgaaagac ttgtatcagc tgaatacccc aatgctcttg
     4801 catgaccatt cctgaaccaa taagctgagg caaaacgaac aaccgcatac taaacaagat
     4861 acctagcaaa aaggtgagca atagcatcgg atatgcaagc agctttttca aatcattgcg
     4921 aaatccttca acaagccgaa actgtttggc aatttccaac aaagttgtcg gcagattccc
     4981 atgacgttca gctaactcga tctgtaccag ttgctctgct ccatatccta tttgcgaaag
     5041 tacctcacta aacgcttgcc cttcctttag ctgatgttgg atatttgcca aaataacagg
     5101 tggaaacgaa cggatcgtga gtaataaata aagggattct tgcagactaa aaccagataa
     5161 aagcagttct cccatggttt gtgcaaactg gttttgctgg cttcttgtta atcctttaga
     5221 atagttctga ctgccaaata ttttcctgag cttcttcata actttcctcc catgattttt
     5281 ttactgattc ttcctccata aattgataac tccataaaac agaaggatgg tgattggcat
     5341 cttccaaaag ttcttgataa atgacaccag ctaaacattc agatagctct tcttttttct
     5401 ttgtcagttc atggattctg accaatgtac cttcaagatt gcgtgcatgt acggttgcaa
     5461 aaacccggtg ccctgttagt gctgctctta ccgcagcatg tgcagttgat tcatctctta
     5521 tctcgccaat gattagcaag tctggacgat gccgtaatgc gagtctcaaa agtgtatcat
     5581 aagtctgtgc tattttcgga ttgatctgta gctgcagaaa aaacggttct tctatctcta
     5641 caggatcttc gatagtaatc acttgcaatt tttcttccaa agccaaccga tacattagag
     5701 aagtcttgcc tgatccgact ggtccgctga aaaggtataa tccacgtctg acggttttct
     5761 gtaaaatagt tgcaaaatct cgagaactaa agcaacgatg gggtgtttca aaagtaccgt
     5821 ataatagccg aatcactaaa ctctctcgtt gaaggtaatc tcctacagaa gacaaccgca
     5881 gacgaacttc ctgtttttct aaagaataag taaatgcacc taactgagct tttctttttt
     5941 ctccgatatc catctgtccc aaatacttaa atcttgagat cagttgctgt ccatctgccg
     6001 aagagacttt cttccagaat gttcgttttt ttccatagcg aaaatagatg taaaatactt
     6061 ctgaagctgg caatatatat acgtcttggg catgtttggc aaaactttca cttatcattt
     6121 gatccgataa tcgttttatt tccattattc tcccctccac aaaaaacata cgtctttttg
     6181 atctttttgt ttcaaataaa atgaatttac aaaaaaatat tggcaaaaac cgaatttatg
     6241 ttatacttat aaaaatttat cttgggaggc actccattgg aaacaacatt aaggaaatat
     6301 atcgaagtag caagtaaaaa aaagcctgct gacctagtga tcaagcatgc taagatcgtc
     6361 aacgtcttta cgaaagaaat catggaaaaa gatgtagcaa tctgcgaagg aatgatcgta
     6421 ggaatcggtg aatacgaagg aaaaagcgta cataatgcta atgggcaata tctagtgcca
     6481 ggattcatcg atggccatgt gcatatcgaa agctcacttt tatcaccaag cgaattttca
     6541 aaagtatcct tgcttcatgg agttactact gtagtgacag atccccatga aataggaaac
     6601 attgctggga gtacaggtct tgagtttatg atagaagatg caagacaaac actcatgaat
     6661 atctttttta tgctaccttc ttgtgtacct gtaacccctt ttgagaccaa tggtgcaaat
     6721 ttaaatgcaa aaaaattagc ccctttttta caaaaacccg aagttttagg cttagctgaa
     6781 gtcatgaact atcaagcagt agcaaataat gaaacagaca tcctagaaaa gatccaattg
     6841 atgcaccagc ataagaaaaa aattgacggg catgcagctg gtatcggcat ggaagagtta
     6901 aatgtttatc ctgcagcagg tattcggacg gatcatgaag ctacaactgc aaaagaagct
     6961 aaagaacgtt tggatcttgg tatgtattta atggttcgag aagggaccgt tgcaaaagat
     7021 ctggcatcgc tactaccggc gatcacacct gaaaattcaa gaagatgctt ctttgtcacc
     7081 gatgataaac tgatcaatga tttagtaaaa gaaggatcaa tcgatcatat cgtccgtcaa
     7141 gctattgcac ttggaatcga tccattacaa gccattcaga tggcaacatt gaatgcagca
     7201 gaatgttttg atttgaaaac actaggtgcg attgcccctg ggtatcaagc tgatttcttc
     7261 ttaacagacg atcttaccaa gctgccaatt catgaagttt tctataaggg aacattagtt
     7321 gttcaatctg ggcaactcca agaccattta tttgctgatc attcaaattc atatacgtgt
     7381 tgtctgccaa aactaaacgc tcacccatta aatccacatt cgttagagct gcttctttct
     7441 tcttcacaag cccatgtgat cgaaattatc ccgaatagtc tagtgactaa agatttggta
     7501 gaaaaagtgg accgacaagg cgaacgattc attccttcaa tcgaaaaaga ccaattaaag
     7561 attgcggtga tcgagcgtca ccatcaaacg ggaaacattg gattaggcat agtgaaagga
     7621 tttcagctga aacaaggagc gatagcgaca acgattgccc atgactcgca taatctagtt
     7681 gtcgttggga caaacgacga ggatatgctg tatgctgcta atcttttgat tcaaaaaggt
     7741 ggcgggatga ccgttgtcaa caaacagcag gaaatagctt gtgtttctct tcctataggc
     7801 ggaatcatga cacaggaacc tttttcgatc gtctatgaac agttgaatga attaataact
     7861 aaagcacatc aactaggagc agctaaaacc tttgatcctt ttttgacact ttctttctta
     7921 acattatctg tcatacccga attgaagatc accgacaaag gattattttc attttctcgc
     7981 ttccaattga ttccaccttg tggaaaaacc gatgaaataa atacatttta aatcaaataa
     8041 atcccagaaa atacattaca tctgtaatgt tcattttctg ggatttattt atggatatta
     8101 atgtttttat gaaagcagaa agttagtttc acagactttt ctttttggtc ttatcttgaa
     8161 attgagcttc tactcgataa atacgttgac cttttgaaga aaatttttct tcatactccg
     8221 tcataatatt gccttcaaat tgactttgat gtaaatctaa ccatacctgt ttgaggatca
     8281 tgccatattg agaaaaactt gccagagaat actcaaacaa tccttgatta tctgttttga
     8341 aatggatttc tccatttgga cgaagaatct gttcatctac agcaagaaaa gtcttatatg
     8401 tcaatcgccg tttttcatga cgtttttttg gccatggatc ggaaaaattc agatagattt
     8461 gatctacttc gctgtcagcg aaatactctg tcaacgctga tccatccaca tgtagaagtt
     8521 tcaggttagg aagcggttgt tcgatcaact tgtctagtgc aatcgaaaca acactgacct
     8581 gcatttctac tccaatataa ttgatttcag gatgggcttt tgccattcca gtgatgaact
     8641 ggcctttccc cataccaatc tcaatgtgga ttggatgatc gttttcaaac aattcattcc
     8701 attttccctt ccacaatgtt ggatcactaa ttacaaaatt cgggtgagcc gctaacattt
     8761 ccgctgctcc cggacgattt cggacacgca ttatttatct ccattccttt cctctaatta
     8821 aaaaggtctg gccaaatctc cgcagagttt gggccaaacc tcagccactg aaaattcgtt
     8881 ttaattgtaa aatttcttcg ttcatccgtc gatgatcttc acgtttgcaa tatctggtga
     8941 tttcttggag caaattcatg gccgcatacc aatgaaattt ttcaagcatt tcgtcagtcg
     9001 ggcgtatgcc ataactgatc agccactgat tccagctaga cagtggcaca taatttccta
     9061 aaatcgttgc aatatccacc gcagggtctg caaacatgat tgaatcccaa tcgactagat
     9121 ataaataatt tttacatact aaccaattac gatggtttac atctccgtgt actactgata
     9181 agttttcctt atctattgac gggatctgct cacgaagaaa acgatggaca atttgcagaa
     9241 attgattttt ctggagttct tccggtaatt ctttttcata tttttgcagc atcttctccg
     9301 gcgtcatgac acgaccaccg atacgggtca gcatggtttt aagagaattt gaatgatgta
     9361 aatggtacaa aacatcaatc acgtcgtttc tttgaccaat ctcattagga gtcagcaagc
     9421 gaccttccag ccactcttgt gctgtcaaag tatcgccatt ccccgttcgt tttgtccaaa
     9481 caagttttgg tgtaattcct tcccttgaaa gagcagccaa catcggagtc gtgtttcgtt
     9541 taataaagac tttgtcctca tcacgcatgc ctatgtatgt ttttcctgtg tcgcctttga
     9601 tgggacgtaa ccgccactct tggtctaact gaaactccat catgcattct cccgtcctct
     9661 tactctttgt tttgcagtac ttattattct agtcgcctga acactacacg tcaagcacat
     9721 atacgttctt tttcatttaa ttgccattca aaaaaccagc ttttctcaaa aaaaatagaa
     9781 cgaacagcat tcttactgtc aacgctctat ttttcattct catttttacc ctacacagct
     9841 ttgttttcag tctgacatct tcttcaaacg agttggtaca tatagtttaa caaagagcat
     9901 agtaacaatc aaataagccg ctagcggaac ccatcgctgc tgcccatcta atacgactgc
     9961 agcaatcaaa ccaaaaataa ccgacgcgat aattaatacc catgtgagta gatactgaat
    10021 cgcctgcttc ttctgattct tatggattgg ataaagctga acgagaacca tatagcggaa
    10081 ctggttcgct aaaggtaaca gttggaaacc gatcaaatat aaaaataaga aaccgataat
    10141 cagactaaaa taaaagtcga ctactccggc aatcaataac ccacctataa ttactaatcg
    10201 gaaataaaga ccactaaaat cagaacctct tgccaatctt cttgcaaaca agtaaagata
    10261 agtgtttttt gattctcttt taatatgagt caatatagga tccaaatagc gtcttctttt
    10321 gatctttgcc gtaatttcag gaacatctgt gaataaattg ataaattgat agatctgatg
    10381 caacctatgt tcttctacat gcaccatttt ctcccaatcc aatccttttt cttgttgctt
    10441 ccacaaaaac cagtaatata gacttgcctg tactaatgca cctgctattc ctgcccatat
    10501 tagccaataa aatgatagaa acaaaaagat cagagaagaa aaaaaccagc ctaatttcca
    10561 ataacgaata acttgttgat ctagtcgaaa caattctgct ctcttgattt gcagatgact
    10621 gaatttcaaa ctccataata tgatcagata gacgaaaaac atcgaaaagg attgctccgt
    10681 actaaccacc actaatggca tggccaatcc gcatgccaat atcaaaaaga tcaacggaaa
    10741 aatgcaagaa tagcgaaaag ctcgttccaa ataggtttgc atttcttttt ctttaggaag
    10801 taaaaaaaca atatctgctg cctctgcaaa agtagccatc ttacccacga ataaacagag
    10861 catccaaaat acagagatta tgagccctcc aattgcaaac ggtggctgca gggttttcaa
    10921 ccaattagaa taatataatc ccacaccgcc aatcaagaaa agacaaacta aaacaaaatg
    10981 atcgttcaat acataacgca tatatttcat cattttcttt tgatgtcttg ctaaacgttt
    11041 ttgaaaaaaa ctactcatgt gcctgctcct ttgttaaagc aatatagatt tcgtccaaag
    11101 aagaatccgg cagctgaaat tcttttctca acatctcaag agaaccgtct gctcgtatct
    11161 ctccgttatg taagaccaca aaacgatcac aatatttttc tgcagtagct aaaatatgcg
    11221 tagacatcaa aataccggct ccttcttttt tcatttctac catcaattca agcaatgcat
    11281 tgatagctag tggatccaag cctaaaaatg gttcgtcgat gatatataaa gaaggctcga
    11341 tcaaaaaggc gcataagatc atcactttct gtttcattcc ctttgaaaaa ttagctggga
    11401 accagtcaag acgattagat aaccgaaaag tctccaacaa cttttctgct cttttcattg
    11461 ctatatccat aggtatatcg taggccatcg cagtgacttc gatatgttct ctcaatgtca
    11521 attcttcata taaagaagga gtctctggaa tatacccaat ttgtttgcga tattcttccg
    11581 tatttttctt taaggataga tgattgattt ctatcgttcc ttttgaagct tccagcagac
    11641 cgataatgtg cttgatggtc gtacttttcc ctgctccgtt cagtccaatc aaccctacta
    11701 tctctccagg ctgtacatcg aaactgatat cttttaacac agggagatgt ccgtaccccc
    11761 ctgtgacatg atcgataatt aaactcattc tttttcctcc gccatttatt tttatttatt
    11821 ataccacgaa gaaaatgacg gtattttgtt tttttcctta caatagcttt aacttatggt
    11881 aaagttaagc tgaagtattt attctttata gaaaggatgt tttatatgga aaactgtatt
    11941 ttttgcaaaa tcatcaatca agaaatccca agctataaaa tatatgaaga cgacaaggtc
    12001 tatgcttttt tagacatctc tcaagcaacg aaaggacaca cattagttgt gcctaaacgt
    12061 catgtagcag atatttttga atatgatccc gaacttgcag gagaggtttt ttcgcgtgtg
    12121 cctaagattg ctcaagcatt ggaaaaagct tttccagaaa tgaaaggtct taatatcttg
    12181 aataacaacc gtgaactagc ctatcaatct gtttttcatt cacatatcca tttagtccct
    12241 cgctacagca aagaggatga tttttctatt cactttgtca atcatcaaga tagttatgga
    12301 tcggaagaac taaaagctat tcaagaaaca atcgtaaagc aggtgtcctg cgatgattaa
    12361 aaactttttc aaaggactgt tatttggagc tgctgctggc actgtcggag gtctgttatt
    12421 ggcgcccagg agcgggaatg aaacaagaaa gaaactgatc gaagaattgg aagaagcaac
    12481 aactctgaca aatgatttaa ataacagcct gaatcatttt aaaagcgcat tgatcgaaac
    12541 aaaagaaaca gctgaatctg tgattcctgc tttccaagaa gcaatcaaaa aagatatcga
    12601 agattttaaa ttccaagccg aaccacgtat cacacaaatc aatgaacaag tagaaaaact
    12661 aaaagctgat ctgccagagt atcctgaaca aatataataa tgattatttt ctcaacagat
    12721 ttaactatat aagtgagaaa agtttgcgta tttcctagga atgtgatgaa attttgaatt
    12781 ttgtttgaaa ctaccacgac aacgttttta aaggaaatgg gatatggtat agttaatata
    12841 taaaaataat aactaattac aggagtgcaa attttaatga aaaaaaaatc aattattttg
    12901 gcagctacta gtgcgttggc tgttttaact ctagcagctt gttcaggcga tactaataaa
    12961 gatatcgcaa cgatgaaagg cggaacgatc actgtatctg atttctatga tgaagcaaaa
    13021 cttgaatcat cgaaccaatc cttagtacaa cggatgatca tttataaagt attcaataac
    13081 aaatatggcg ataaagtaac cgataagcaa gtggatgcag aatacgacaa acaagcgaaa
    13141 tcattaggtg atacttttga atcacaatta gaagctgctg gatatacgaa agatacttat
    13201 aaagaatata ttcgtaacaa tttagctttt gaagctggct tgaaagctca cgtagatatt
    13261 acagatgacg atttgaaaac tgcttggaaa tctttccatc cagaagtaga agcacaaatc
    13321 atcaaattat caagtgaaga tgaagcaaaa gatgtgaaaa aatcagcgga tgatggagat
    13381 gatttctcta aattagccaa agacaaatca actgatacag agacaaaaga agatggcggt
    13441 aaagttaaat tcgactcaac gacaacaact attcctgcag aagtaaaaga agcagctttc
    13501 aaattgaaag acggtgagat ctccgatgtt attactacaa caaatcctac ttcttatgcg
    13561 acagaatact acgttgtcaa aatggtgaaa aaccaaaata aaggcaatga catggataaa
    13621 tacaaagatc aactaaaaga tatcgctact gaaactaaac taagtgacaa tgcattcaca
    13681 actaaagtaa tcggtgaaga attaaaagat gcaaatgtaa aaatcaaaga tgatgccttt
    13741 gaaaatgtgt tgagtgcatt cacaacaact tcatcaagta cgaaagactc aagtgaaact
    13801 acagcttcaa caaagagttc tgacacaaaa tcaactgatt caacaaaaga atcttcaact
    13861 gaagaaacaa ctgattcatc aaaataaaag aaaacaaaaa aagcgaagac ttatgtctcg
    13921 ctttttttgt tttgaatgaa aaaaataaat ttttgctata aatcttttgg tacatagaaa
    13981 cttcggttgt ccataccgaa aatccgatcg gtgtattttc ctggttctgt ttgacgaatc
    14041 gaagtcaaca tcatctgcat cgaagcatcg atattgtcga tttgatgtaa tatttctgct
    14101 tccattattc ttggacgtac aggtgagcca tattctaata agccatggtg tgctaaaacc
    14161 atatgacgta atacgagaac atcttcgtca gcttcgctga ttttcaacgc aagacaggct
    14221 ttagttattt cttcgtcaat caataccaga tgaccgatca aatttcctgc aagcgtatat
    14281 tctgtcgaca taggaccgga aagttcaatc acctttccta aatcatgcaa aatgatacca
    14341 gcatacaaca atgctcgatt cagctcagga tattcatcgg cgattgattt ccctaagtga
    14401 agcattgtca aagtatgata agctaatcca ctggcaaatg catgatggtt tctttttgct
    14461 gccggaaact caaaaaattg tttttgatat ttattaagta gatagcgaac aatacgattc
    14521 caatgcgcat tggtgatctc aaacaacaat tgattgattt cttcttccat ttcttctttc
    14581 ttcaaagggg ctctttccat gtataaacta ggctctcctg gctcatctac ccttgttaag
    14641 cgcatatgaa gaatctttac ttgagggttt ccttgataaa cctctctttt accgtttaga
    14701 aaaacgactg tgccggctgt aaatttattg atttcctcct ccgaagcatc ccagtattta
    14761 ccatcgatag tacctgaagt atcttggaaa gtaaaagcaa taaatttttt tccgttttta
    14821 gcgattctta catctgcact tttgatcagg acaaattgtt caaactgctc atcgattact
    14881 aaatcacgaa tttttttcat tatttgctcc ttctaattgt tttacgattt ggttcatctc
    14941 atggtaatag cttaccattt ctttatcaga agaaaaacaa attatttgat acttttctcc
    15001 aaatttagct aaaagttcag ctaattgata ctttcgctga tgatcataat gaagccagcc
    15061 atcatcaatg atgatcgggc atatcgaacg gttagcttct aatgagaggt aagcaaatct
    15121 catcgccata atcagctgat ctttcgtacc tgtagataac tcatagatgg gaaattctgt
    15181 atatccgtct gttaaagtca gtgtatcatt tttcagtaat atcttctgat aatttccttt
    15241 tgttaaaata cctaaataat cacctgctgc tcttaacaac tggggtaatt gtctttcact
    15301 catctctgtt gaaagatcac ctaagaatgt cccggcgatt tcaagtgctc cccattcctc
    15361 cagtaattcc tgtaattttg ctttttgccg gctagcttgt tgataaagtt cgtccaatgt
    15421 gccgtctctt tgcaactctt ctacttgaag ttggagtcgt tgccgatttt cacttgcttc
    15481 tttttcttct tgctggagtt gggattgctt ttctttcagt tggcgcagtc ttacagataa
    15541 tttctctttt gtcagctctt caggaaatag tggagtaagc atttccgata attcattgta
    15601 tcttttcatc tgctgttcgg cttgtttggc tttttgtaaa aagacaggta cttccgaagg
    15661 ataagataag cctgcttgag tcaattgttc gctgtattct tgtatccata ctttttgttt
    15721 ctttttcaat tgattgattt cttgcttcaa taaagtattt tgctgatagc ttctggcaaa
    15781 tttcatctgt tccatctctt gtataaaacg ggaaagcaat accatttttt ctgaaattgt
    15841 cttatcttgt atcggtaacc attctttaac aaactcaaat ttttggtcga tttgtcgtag
    15901 tttttcttgt atttcctgtt ctcttttatg tttccgctca aatacgccat ataatttctg
    15961 ataatctatg acttcctgtc catattcttt catgagtttg agcgtatcca tctttcctaa
    16021 gtgccgatga tccgcaaaac gatatacttc ttgctgaaga atctgttttt cttctccagt
    16081 tctcttttgt ttttcttctg cttcagctaa ttgagctaag gctagatcaa gttgtcctag
    16141 tttttcttgc cacattcctt ttatctcaga gaaatcattt ttagttttaa tttttggttg
    16201 ccttttgtag aagaaatagc caatcatgcc tgcaccaatt agtaaaagga tcactttcaa
    16261 gggcattggt acaaatatac ctgcaatgat tattgctccg ccagccatca gcatatacgg
    16321 tttagatgaa gagactggtg ctatctctga ctgattttgt aataatgctg gataggtctc
    16381 ttccaagtca ttcacttctg cttcaatcat ctcttgctgt tctttagcta tttggagacg
    16441 cacagaaatc tggctttcta gatcagcaat ccttctttct tcagccagaa gatgcttcca
    16501 ttcttcttca tataaaaatt cttccggtaa cggatttttc tgccaatgcc acctttgttc
    16561 caaaatcttc aattctgaat ttgtttctct agtctcaatt tgtaatcttt gccattcttc
    16621 gtttaattgt tccgctgtaa attgaagttg ctggagatct tttagttcct gctcctgctc
    16681 taaataaaaa taatatctgg ctgactgctg atccatcccg ctatgctttt ccagtgttgc
    16741 ttctaattct tccaaacgct catttgtttg ctgataccgt ttggagaaag actccagatc
    16801 taaaagaagt tgctcatcag gaagacctgt agtgctgttt tttttgagag attgccattc
    16861 ttcatataaa gaaaaattaa gttcttgctg tctggctaat gaaaattgtt tgttcgcctc
    16921 ttttaattgt gccaataact gctgctgttg cgactcgtat tcctgttctt gttggatcaa
    16981 cttgccaaaa taagcttcct gttcttgttt ttgatgaatt ttttcttgca gctctttcca
    17041 ttcatttaat ttttgattga ttagtaattt ttggcctttt ttcttgaata attgttgtgc
    17101 tttctgataa tattcttgcc gcttttggaa tacttgagaa ctgcccgtga tgcctaaaga
    17161 aatcagcgca tcatgtagct ctttttctct caaatgatct agttccatca actgctcttg
    17221 ctggaaagta aatacctgct ggaataactc tttcgtcaaa ggtgcgatca gtttttctag
    17281 taactcatca cttcccactt gatcccccag ccacaccttc gattttccgc gattttgctg
    17341 cttgtagcgc tcgacagcaa attcaccata tacaggatgt ttaagccata ttttgccgcc
    17401 ataggctgag ccgtcttttg gtgtataatc ctttttttta cggccttttg aagggaaacc
    17461 aaataatatt gcttgaataa attgataaat cgttgatttt cctgcttcgt ttcccccata
    17521 gatcagctga ttgcctgaat aaaagtcaaa acttttttga cgccattttc caaaagcagc
    17581 aatctcgatc cttaaaatct tcattcctga tcctcctcaa ataaaaaatc ttgattaagc
    17641 agctgcttgg ctttttcaag tgtttcttct tgatattccg gcagttcgct caatattcta
    17701 gccgcttctt gatgactata tacttcattt aaaatttgtt ggaaatcttg aggtgtctga
    17761 tagtattgaa ataactgttc catcaattta gcagaagcag caagtggggt cttcgtataa
    17821 aaagtattct ctatgagaga aatacgccaa ataaagaaat catttatcct atctgagaat
    17881 tcctctaaca aatagtctaa tagctctcct gattgtatcc gttccaaaac atctgtattg
    17941 agatggtcat aatcttttaa ttttatctca attaaactaa atccatcctc tacttttgaa
    18001 agctgctgac gaatgtgaac aatgacctct tttgtcgttc ttgctttcct caatgatatt
    18061 tcctttgtct tccaatagac ctctgctact ttaatcggat gtatttggca acttccttta
    18121 cttaattcaa ctagtagaat agatgtagaa gcttgttcct ttttagtatg accttgaggc
    18181 gcacctggat aaacaattgt ttgcttttca tttaagaccg ttggcacatg gatatgccct
    18241 aaagcccaat aatcgtatcc cttttcttgc atctttgagg gttggaaagg agcatagttt
    18301 cctttttgag agacgccagg ttccccgtgg tacaacccaa tatggtaatc tgtgagttct
    18361 ctagaaggaa attccatcac tttgtctttt tgaatccacg gttgacgata actaaatcca
    18421 ctgagagaga cagtttctcc actttttatc gttatttttt ttgtctctac cgtttcggat
    18481 gtaaataaat ggacattttt aggaaaggag aaccaatacc gctctttttg atagaaatca
    18541 tgatttccga atattataaa tacaggtata tggttctttt ctaaccgttt catctgttct
    18601 gaaaaatgtt tctgtatttt taatgaagga cgattttgat gaaaattatc tcctgcaagt
    18661 aaaacaaaat caacgttatt tattattgcc tgttcaatga tatttgataa aacggtcaga
    18721 ttcgtcttca atagtttttc ctgtacagtt ttatcaagag ttgtcagtcc ttcaaatgac
    18781 cgatccatat gtaaatctgc cgcatggata aatttcacca ttaaaaattt cctccttttt
    18841 ttcttttatc ataccatttt caatgttttt tttcatcaat aaccgaaaat ttgttcgtat
    18901 tatgcaaaga aattacgata aatggccgac ttcaaaaaat aagtttattc gaaaaaaaga
    18961 ttgtgacatg atttttgtca caatcctaaa tatctatgtt atattcgctt cgtttgacac
    19021 tgaagaagta ctatttgtac tggaatttat gctccatttt tccgtattta gaattctatg
    19081 ttctatacta ccttaattag tttatatggt aagaattctt tcaaatgctt gagatactcc
    19141 gtctttatca tttgaaacag tgacataatc agcttttttc atgatttctt gatgactatt
    19201 tcccattgca aaactaatat ctgcactttc aaacatagat aagtcattta aactatctcc
    19261 gatcgcgatt ctcaaaggtt tctttaaatt gtagattgat tccaaacgat caaacaccac
    19321 acctttagat acattttgag gtacgatttc aatatttaca ggtgccgatt cagtaacaga
    19381 cacatttagc atctttttca aatggttaaa tatattagct ttcaattgac gattaggaat
    19441 aaaaataaaa aatttgtaaa tgctgatagc atccttttct ataatttcct caatattatt
    19501 ataagggatt gaacaaatag atttttcata ctcaagctca tagtttagct ctttgataga
    19561 aaattcactc cctacctcgt gaaaaatttt tttcatttta tttttataat tctgagattc
    19621 ataacttccg agatttgtat atattttggt tggaaatttc gataggacag aatcaatttt
    19681 ttttacatca tttttatcga aggaagactc aaatatcttt ttttctccat caaatccaac
    19741 agaaccattt aaccctactg ctggtatcct taaaccagat tttttgagaa tattattaat
    19801 gtcttctact tctcttccag aacaaatgaa tggaaaacag tcttccggta aatgtttcat
    19861 tgttcttgca tttttttgac taatgctctg gtcagaattc aaaagagtac cgtctaaatc
    19921 tataccaatt agtttcattt catccatgtc tcctatacgt tgattataaa ttcactgctt
    19981 attgtaagtc taaatttctc acaaaacata tactaattat acctaattta agtaagtaca
    20041 aacatcattt taagaatagg aaaggacatt tatatactat tctctgtatt tttgatttat
    20101 aaataaatat gaattgaatc caaatcagct atattactat tttataataa ccaactataa
    20161 aaaacgaagt taggtgaaaa actaacttcg atattcataa aagactctat tagttcaact
    20221 tagcaaaaga tttaaa
//
LOCUS       DAYYGY010000056        19846 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000056 DAYYGY010000000
VERSION     DAYYGY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.057"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..361
                     /locus_tag="ACHA52_001914"
     CDS             167..361
                     /locus_tag="ACHA52_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940607.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            630..929
                     /locus_tag="ACHA52_001915"
     CDS             630..929
                     /locus_tag="ACHA52_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940608.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1026..1460)
                     /locus_tag="ACHA52_001916"
     CDS             complement(1026..1460)
                     /locus_tag="ACHA52_001916"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8940609.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="ACHA52_001917"
     CDS             complement(1692..2402)
                     /locus_tag="ACHA52_001917"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8940610.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="ACHA52_001918"
     CDS             complement(2392..3252)
                     /locus_tag="ACHA52_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8940611.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="ACHA52_001919"
     CDS             complement(3257..3895)
                     /locus_tag="ACHA52_001919"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8940612.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="ACHA52_001920"
     CDS             complement(3910..4209)
                     /locus_tag="ACHA52_001920"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8940613.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="ACHA52_001921"
     CDS             complement(4252..5736)
                     /locus_tag="ACHA52_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8940614.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="ACHA52_001922"
     CDS             complement(5756..6217)
                     /locus_tag="ACHA52_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8940615.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="ACHA52_001923"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="ACHA52_001923"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8940616.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="ACHA52_001924"
     CDS             7520..8290
                     /locus_tag="ACHA52_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8940617.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="ACHA52_001925"
     CDS             complement(8563..9390)
                     /locus_tag="ACHA52_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940618.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="ACHA52_001926"
     CDS             9958..11193
                     /locus_tag="ACHA52_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8940619.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="ACHA52_001927"
     CDS             complement(12192..12452)
                     /locus_tag="ACHA52_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8940620.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="ACHA52_001928"
     CDS             complement(12479..13780)
                     /locus_tag="ACHA52_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8940621.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="ACHA52_001929"
     CDS             complement(14072..14530)
                     /locus_tag="ACHA52_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8940622.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15874)
                     /locus_tag="ACHA52_001930"
     CDS             complement(14543..15874)
                     /locus_tag="ACHA52_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8940623.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17327)
                     /locus_tag="ACHA52_001931"
     CDS             complement(15885..17327)
                     /locus_tag="ACHA52_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8940624.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="ACHA52_001932"
     CDS             complement(17314..18078)
                     /locus_tag="ACHA52_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8940625.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18182..19057)
                     /locus_tag="ACHA52_001933"
     CDS             complement(18182..19057)
                     /locus_tag="ACHA52_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8940626.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19312..19396)
                     /locus_tag="ACHA52_001934"
     tRNA            complement(19312..19396)
                     /locus_tag="ACHA52_001934"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19360..19362),aa:Leu,seq:caa)
     gene            complement(19414..19485)
                     /locus_tag="ACHA52_001935"
     tRNA            complement(19414..19485)
                     /locus_tag="ACHA52_001935"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19451..19453),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="ACHA52_001936"
     tRNA            complement(19495..19567)
                     /locus_tag="ACHA52_001936"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19629..19713)
                     /locus_tag="ACHA52_001937"
     tRNA            complement(19629..19713)
                     /locus_tag="ACHA52_001937"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Leu,seq:caa)
     gene            complement(19734..19804)
                     /locus_tag="ACHA52_001938"
     tRNA            complement(19734..19804)
                     /locus_tag="ACHA52_001938"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19770..19772),aa:Cys,seq:gca)
BASE COUNT         6306 a         4097 c         3083 g         6360 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      601 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      661 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      721 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      781 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      841 taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcatcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaaa ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
    18181 tttaatagat tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
    18241 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
    18301 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
    18361 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
    18421 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
    18481 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
    18541 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
    18601 cttctactgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
    18661 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
    18721 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
    18781 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
    18841 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac
    18901 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
    18961 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc
    19021 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
    19081 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
    19141 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
    19201 ttttttactc gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa
    19261 acaaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
    19321 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
    19381 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
    19441 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
    19621 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
    19681 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
    19741 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
    19801 cgccattata ctattatcaa tctataaaat aaagaataaa ttatag
//
LOCUS       DAYYGY010000057        19629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000057 DAYYGY010000000
VERSION     DAYYGY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.058"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHA52_001939"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHA52_001939"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8940627.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHA52_001940"
     CDS             complement(1172..1867)
                     /locus_tag="ACHA52_001940"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8940628.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHA52_001941"
     CDS             complement(1880..2596)
                     /locus_tag="ACHA52_001941"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8940629.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHA52_001942"
     CDS             2926..3534
                     /locus_tag="ACHA52_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8940630.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHA52_001943"
     CDS             3859..4182
                     /locus_tag="ACHA52_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8940631.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHA52_001944"
     CDS             4364..5734
                     /locus_tag="ACHA52_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8940632.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHA52_001945"
     CDS             complement(5864..7165)
                     /locus_tag="ACHA52_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8940633.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHA52_001946"
     CDS             complement(7519..9510)
                     /locus_tag="ACHA52_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8940634.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHA52_001947"
     CDS             complement(9654..9920)
                     /locus_tag="ACHA52_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8940635.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHA52_001948"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHA52_001948"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8940636.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHA52_001949"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHA52_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8940637.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHA52_001950"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHA52_001950"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8940638.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHA52_001951"
     CDS             complement(14080..14601)
                     /locus_tag="ACHA52_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8940639.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHA52_001952"
     CDS             complement(14604..15446)
                     /locus_tag="ACHA52_001952"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8940640.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHA52_001953"
     CDS             complement(15439..16164)
                     /locus_tag="ACHA52_001953"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8940641.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHA52_001954"
     CDS             16340..16636
                     /locus_tag="ACHA52_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940642.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHA52_001955"
     CDS             17204..18796
                     /locus_tag="ACHA52_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8940643.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHA52_001956"
     CDS             18809..19549
                     /locus_tag="ACHA52_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8940644.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DAYYGY010000058        19319 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000058 DAYYGY010000000
VERSION     DAYYGY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.059"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..452)
                     /locus_tag="ACHA52_001957"
     CDS             complement(<1..452)
                     /locus_tag="ACHA52_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8940645.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
     gene            complement(557..1420)
                     /locus_tag="ACHA52_001958"
     CDS             complement(557..1420)
                     /locus_tag="ACHA52_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8940646.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1616..3715)
                     /locus_tag="ACHA52_001959"
     CDS             complement(1616..3715)
                     /locus_tag="ACHA52_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8940647.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3935..5044)
                     /gene="rpoD"
                     /locus_tag="ACHA52_001960"
     CDS             complement(3935..5044)
                     /gene="rpoD"
                     /locus_tag="ACHA52_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8940648.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5068..6909)
                     /gene="dnaG"
                     /locus_tag="ACHA52_001961"
     CDS             complement(5068..6909)
                     /gene="dnaG"
                     /locus_tag="ACHA52_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8940649.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7091..9406)
                     /locus_tag="ACHA52_001962"
     CDS             complement(7091..9406)
                     /locus_tag="ACHA52_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8940650.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9591..10451)
                     /locus_tag="ACHA52_001963"
     CDS             complement(9591..10451)
                     /locus_tag="ACHA52_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8940651.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10510..11142)
                     /locus_tag="ACHA52_001964"
                     /pseudo
     CDS             complement(10510..11142)
                     /locus_tag="ACHA52_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            11309..12070
                     /locus_tag="ACHA52_001965"
     CDS             11309..12070
                     /locus_tag="ACHA52_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8940652.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12096..14687
                     /gene="mprF"
                     /locus_tag="ACHA52_001966"
     CDS             12096..14687
                     /gene="mprF"
                     /locus_tag="ACHA52_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8940653.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14875..16062)
                     /gene="tuf"
                     /locus_tag="ACHA52_001967"
     CDS             complement(14875..16062)
                     /gene="tuf"
                     /locus_tag="ACHA52_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8940654.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16430..16786
                     /locus_tag="ACHA52_001968"
     CDS             16430..16786
                     /locus_tag="ACHA52_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8940655.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17027..18031)
                     /locus_tag="ACHA52_001969"
     CDS             complement(17027..18031)
                     /locus_tag="ACHA52_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8940656.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18197..19048
                     /locus_tag="ACHA52_001970"
     CDS             18197..19048
                     /locus_tag="ACHA52_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8940657.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19065..19310
                     /locus_tag="ACHA52_001971"
     CDS             19065..19310
                     /locus_tag="ACHA52_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940658.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         5388 a         3992 c         3380 g         6559 t
ORIGIN      
        1 tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc ttttactaaa
       61 aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc ttctacttcc
      121 tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc gccttctttt
      181 ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat tttcaattct
      241 gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact cttttttttc
      301 actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa aagcacaagc
      361 agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg ctgttttgtt
      421 tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat ttataataat
      481 tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct agttaagaaa
      541 aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg ttttcttgtg
      601 taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct ttgctgatac
      661 caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt tgctgatctg
      721 cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct tcatatgctc
      781 ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact cgacctttta
      841 tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac ccaagataga
      901 aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt cctatcacat
      961 ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt ttttcatctg
     1021 ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc aagcgatccc
     1081 cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg acaaagtctt
     1141 tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca gtaacgattc
     1201 caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga ttttcatgtt
     1261 tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca ccttctgatt
     1321 tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca ttttcatcaa
     1381 taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc ctctatagtg
     1441 aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac ttgaatagct
     1501 gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca tactcccctt
     1561 tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc taaaattaat
     1621 tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg taaaatggca
     1681 aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca tatagattat
     1741 aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct tcaacgatat
     1801 atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc cacgtccatg
     1861 ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc tgattcgctg
     1921 agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt gcgaccgcag
     1981 cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct tgttcttcag
     2041 ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc tctttatcag
     2101 tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga tctgtatttt
     2161 tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct gaatccacta
     2221 cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat tcagcatatt
     2281 gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg ttatccgttt
     2341 cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga tgatcatcga
     2401 cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc ttagattgga
     2461 tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga ctggataaat
     2521 cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat tgatggtgat
     2581 catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc tgtctttttg
     2641 cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc cagcttcctc
     2701 caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat tttttctttt
     2761 gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt tcagtatctg
     2821 gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc tcttcttttt
     2881 ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca gcatcggatt
     2941 cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt atttcttttt
     3001 tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca gtaaatactt
     3061 cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct ttacctgtgg
     3121 cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct actttgtttt
     3181 cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct tcttttgtat
     3241 cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca ttatcgacga
     3301 tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct tcttgattct
     3361 ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt ttctctatat
     3421 tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt tcgaataatt
     3481 ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga tcgtttaatt
     3541 ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca tctgtatttg
     3601 tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga ccgcagtctg
     3661 gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt ttcatcactt
     3721 agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt ctatacgtca
     3781 cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc ttcctataga
     3841 aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa accaaacagt
     3901 tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct tttaattgtt
     3961 ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga cgaatacgtt
     4021 cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg ccatcatcta
     4081 aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct agtacatctt
     4141 ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt gcttcctggt
     4201 cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt tcaagggaaa
     4261 caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga tccatttccg
     4321 caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga cgttggatac
     4381 gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta cgtgcttggt
     4441 cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt gaaaacttaa
     4501 atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt ccttcttgga
     4561 tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt actactaaac
     4621 gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg attttcaatg
     4681 ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct ttcagataca
     4741 tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct gtttttgctt
     4801 gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca ttttcatcca
     4861 ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca tctgcattca
     4921 aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt cgtttgggtt
     4981 tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct gttccatttg
     5041 ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt tcgttaaatt
     5101 gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc tggcttcttg
     5161 ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga tcacatctag
     5221 caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt cgatttccac
     5281 caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa ggttgaattc
     5341 ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg catcatgaat
     5401 aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa atgcacgata
     5461 taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc gtgcagtttc
     5521 ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt gctgatttgt
     5581 ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa actctgttga
     5641 cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa gttcaactag
     5701 taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc cttgttttaa
     5761 ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt cttggaaaga
     5821 tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa cgactacgac
     5881 agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg ttgcttcaaa
     5941 acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt ccatttgttg
     6001 gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga taccagcctg
     6061 ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt ttttccggat
     6121 atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca gttctgtttc
     6181 tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg caagccagcg
     6241 tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca ctctttgata
     6301 aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc gttctaacaa
     6361 ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct cattaggtgc
     6421 aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt gcaaatattc
     6481 taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt ccgctgcttt
     6541 ttcatgagca gcaatcagtt tccccgtagc agattcagga ttttggacag ctatagtacg
     6601 ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta ccactgcttc
     6661 tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc ctttaccaca
     6721 accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg tacgttcttc
     6781 atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga catattgtcc
     6841 aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt gaggaatccg
     6901 ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca gaagatattt
     6961 caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt aaaggacaca
     7021 attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca catttctatc
     7081 tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca gccttccagt
     7141 acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct acgctctctc
     7201 ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca tatatcccta
     7261 cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa gcttttacaa
     7321 ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt tcagcatttc
     7381 cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact acaagcaagg
     7441 catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt tttcttttat
     7501 cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca atcagcgtga
     7561 tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc gataaaataa
     7621 atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg ttgaccactc
     7681 ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac aataccagat
     7741 tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa tctgcttctc
     7801 ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt
     7861 tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa accgtatagc
     7921 gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg acatacgcat
     7981 cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga ttgtctccag
     8041 aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct tgagcgtttt
     8101 ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct ggtttttctt
     8161 cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca ttcgcttggg
     8221 ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc gctcctacgt
     8281 acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa gaaaatgcga
     8341 ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata gcccgcatag
     8401 tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg atggctttct
     8461 cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca gtaatggtcc
     8521 ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa tacatgtctt
     8581 gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca atacttatta
     8641 gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca ataacagaaa
     8701 ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa
     8761 taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt tcagaacgaa
     8821 tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct gcacctactc
     8881 ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc aattcagctc
     8941 cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt gcttctgccc
     9001 gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt tttaccaaat
     9061 ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg atcactagta
     9121 cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt atttcttgat
     9181 aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg gaatacgcac
     9241 ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac gtcaatatat
     9301 tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca ttttgctggc
     9361 cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc tttttctcca
     9421 ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc aagattaaaa
     9481 acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa aaaagatgcc
     9541 aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct ttacttatat
     9601 tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa aagaacagga
     9661 caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat cataatcgtt
     9721 gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg
     9781 taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc gatcggcaca
     9841 atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa tcccatatac
     9901 accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat caatccgccg
     9961 gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg agcgccgcat
    10021 aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc agaagtatct
    10081 cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat acatccgaca
    10141 aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa aaaagaaagt
    10201 agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac attttctttg
    10261 aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc aaagacagct
    10321 gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa cattcccttc
    10381 acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc cattttttgc
    10441 cttttttcca ctatttgcgc actccttctt tcatttaatt ttattcatga ttgaacattt
    10501 cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat tcattttgac
    10561 aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat tgtaaagcaa
    10621 ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa gtgggctcag
    10681 cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat gattttttac
    10741 ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata aatcgcatcc
    10801 ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat caatactgtc
    10861 agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct gcatgagatc
    10921 tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct cgtagttttc
    10981 catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca ctcgttttac
    11041 cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca gaatgatcat
    11101 gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac ctagttattt
    11161 tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc cttgatttct
    11221 gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac gaaaatgatc
    11281 gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac tttacacagt
    11341 aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga gcgaaattta
    11401 ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg acggcagcgg
    11461 ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt atctagtaga
    11521 tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc agttaatggc
    11581 cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt ttcttggatt
    11641 tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa aagttcatcg
    11701 attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg ccagagtcat
    11761 cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa gtcttcgcaa
    11821 aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa atgatttaaa
    11881 aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca
    11941 ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga aagaacaagg
    12001 acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc aatttctttc
    12061 tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc atacaatggc
    12121 tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt gtcattattg
    12181 tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga gaaacgtttg
    12241 ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc tctgtgttac
    12301 ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag aatcctcgtt
    12361 acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt ttcggcgggt
    12421 ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc agaaacgtga
    12481 tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt tacagtctgc
    12541 tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa tattggatat
    12601 ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact ttcaaaaaga
    12661 aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta gtttcattgt
    12721 tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg gaaatccgga
    12781 ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga atcgtgtcta
    12841 tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg tccgctattg
    12901 gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc tactacatca
    12961 ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact tttgatcagc
    13021 gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc gtggttgtct
    13081 tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa gcttttacag
    13141 agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc caatttccta
    13201 gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac
    13261 gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt ttattaagtg
    13321 attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt cttgcttcta
    13381 aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg atcgatggaa
    13441 tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat ctgccggatt
    13501 tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa aagatttggt
    13561 tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt gttcatttcc
    13621 ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc ctgaagatcc
    13681 ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat aagcggatgt
    13741 ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc ttcaataata
    13801 aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa gcaatcgaag
    13861 cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa acaagcgaag
    13921 agattgttat gattttgcat gaattcggct acgatttcat taagatgggg gaagaagctt
    13981 atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact cgtgctgtct
    14041 tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca ttttctactg
    14101 agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga aaggaaaaag
    14161 ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt gcagttgtca
    14221 aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac acgaacaata
    14281 aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct ggcagtatgg
    14341 atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa tggttcaatt
    14401 tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta caggaaagga
    14461 ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga ttaagagaat
    14521 ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct agagatagtt
    14581 ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt gagcagacac
    14641 aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg
    14701 atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag ttcatgcttc
    14761 tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg
    14821 accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc
    14881 ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt
    14941 cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc
    15001 aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg
    15061 gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt
    15121 tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc
    15181 aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt
    15241 atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc
    15301 tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc
    15361 acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac
    15421 tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt
    15481 atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc
    15541 ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg
    15601 aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa
    15661 gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg
    15721 aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc
    15781 tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg
    15841 tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc
    15901 tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac
    15961 tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat
    16021 gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa
    16081 ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact
    16141 aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg
    16201 tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct
    16261 tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt aactttcttt
    16321 tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca acaactttca
    16381 atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt
    16441 aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag cttatcatta
    16501 tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa tgtcagctac
    16561 ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg cttataataa
    16621 agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat taaagatgaa
    16681 tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg aagaaataaa
    16741 ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa
    16801 attttattag ataaaaagag gctgggacag aagcgtttaa ctccaagaaa taagaaggag
    16861 ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt
    16921 ttgtctcacg ctcttttttt gttatattcg tcttttccga tccttctctt gacacaattc
    16981 atttaactga tcactggtac ttgaacacct aatattctag tcatttttat aaacgattca
    17041 atacttcatc cacatttttg atcatcacgg atatcgtacc atccggatta ttcacaaatt
    17101 caatcaaatc tgggttgcga tatacatcta ctggtacgat cagttcgatc ccgtttgata
    17161 gcttgaattt ttgttttcca aattttttct cagaaatttc ttgtacttct tttacaggag
    17221 gtgcttctgg gacaaatcct tgctcttgta cttcttcttt aaaagccatt tttgccgtga
    17281 tgttttcctt gaagactttt tccgcaatca tctcatgatc caaccgacct ttttcttcta
    17341 tcgtatcata gacagcttct ttcaaatcag ccatgatatc aaattcttct gtctcaaatt
    17401 tttttccaag atgcttggct actttttgga tcacttttac attttcttct aaagaaggtg
    17461 caggaacact ttcgatcact cttccagaaa aataaagctc ttttttgcca gaaaactcat
    17521 attttttttc aattaattcg taggtccaat cagtcaaatt cacagcgaat gcttcatctg
    17581 ctttttgtgt tttcgcccct agaattgctc gattcaaaat caagcggttg tcgatccctg
    17641 cttcgtctgc ttcaacaaag tgagtgtaag cctcacgata attgactttc aataaagata
    17701 agtacatcac tgtatctaat tcatacaaga caacaaatac atctgcacta ggtgcttctt
    17761 cactttcttg gtagatatcg aaccaccgct gaacgattgc ttcacttgct tccacaaaat
    17821 tttcttgagc gattttaagt gattcaacga tcatgctttc ttctgctagt tgtccagttt
    17881 ttgtttgagc agaagagatt ttttggatct tcttttgaag aaaatcacga atatattctt
    17941 ttgtcagatc cagctctttt tgtgaaaaga ctggagaacc agtttctctg tcaataatat
    18001 gcagaattgc tttttttaaa tagatatcca ttttctcatt cccttctgtc ttagtttacg
    18061 gaaaaaaaat caaaaatacc agaatcaaaa tcattttctt ttaaacaaaa atttcaagag
    18121 tagaaacaaa atggattctt tttttcgaaa aatgcgctac acttatagtt gaacgaataa
    18181 aaaaggagct gaaagtatga attcattaac aagcacttat cgtctatcaa atggttacga
    18241 aatcccggta gtaggattcg gcacatggca aacacctgat ggggatgtcg ctgtttcctc
    18301 tgttaaagaa gcgctggctg caggttatcg ccatatcgat actgcccaag gatacaagaa
    18361 tgaagaaagc gtcggccaag cgattaagga aagtggaatt cctagagaag agatattctt
    18421 gacaacaaaa ttatggaatg caaaccacag ctatgaactt gtgatgtctt cttttgaaga
    18481 atcattgaag aagctccaaa cagattattt agatctgttt ttgatccact ggccaaaccc
    18541 tgttgctttt cgtgataatt gggaacaagc aaatgcagat acatggcgag catttgaaga
    18601 actgtatgaa gcaggtaaaa tcaaagcaat cggtgtgagc aatttcttac cccaccatct
    18661 agatacacta gcaaaaacag caaaaattat gccgatggtc aatcaagttt tccttgcacc
    18721 aggcgaatta cagacaactg tagttgagta tgcaaaaaaa catgatatga tcctcgaagc
    18781 atatagtcca ttaggcactg gtaaaatctt cgatgtgcct gaaatgaaac aaatcgctga
    18841 agcacatgac aagacgattg cacaagttgc tttgcgttgg tcgctgcaac acgaattcct
    18901 tcctcttcct aagtcagtga caccaagcag aatcaaagaa aatactgaat tatttgattt
    18961 tgaattaaca gaagaagaaa tgaaacaaat cgatcagtta gatggtgtag tcggcaaagc
    19021 aaaagaccca gatactaccc aattttaagt cgaaaggtga ttttatggat ccaatcgaac
    19081 gcttgaattc tctctcggaa gaagttaccc agacttttca ttccgatttt gtctttttga
    19141 tcgatgcaga gaaaatccaa cattttcctg caagaaactg gacacatgat caaatcatcg
    19201 aagaactgaa aaaacggttc gatcattcgc taatggtcaa accctggcac gaacatgaag
    19261 tcatttattc acctgaacta cctgtttttg ccttgatacc gaaaaaatag aaagaatgg
//
LOCUS       DAYYGY010000059        19222 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000059 DAYYGY010000000
VERSION     DAYYGY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.060"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1570
                     /locus_tag="ACHA52_001972"
     CDS             143..1570
                     /locus_tag="ACHA52_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940659.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1662..2027)
                     /locus_tag="ACHA52_001973"
     CDS             complement(1662..2027)
                     /locus_tag="ACHA52_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8940660.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2024..2389)
                     /locus_tag="ACHA52_001974"
     CDS             complement(2024..2389)
                     /locus_tag="ACHA52_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8940661.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2581..2829)
                     /locus_tag="ACHA52_001975"
     CDS             complement(2581..2829)
                     /locus_tag="ACHA52_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940662.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2828..2956
                     /locus_tag="ACHA52_001976"
     CDS             2828..2956
                     /locus_tag="ACHA52_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8940663.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3013..4707
                     /locus_tag="ACHA52_001977"
     CDS             3013..4707
                     /locus_tag="ACHA52_001977"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8940664.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4774..4980
                     /locus_tag="ACHA52_001978"
     CDS             4774..4980
                     /locus_tag="ACHA52_001978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940665.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5358..6491
                     /locus_tag="ACHA52_001979"
     CDS             5358..6491
                     /locus_tag="ACHA52_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8940666.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6522..6842
                     /locus_tag="ACHA52_001980"
     CDS             6522..6842
                     /locus_tag="ACHA52_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8940667.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6871..7335)
                     /locus_tag="ACHA52_001981"
     CDS             complement(6871..7335)
                     /locus_tag="ACHA52_001981"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8940668.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7467..8720)
                     /locus_tag="ACHA52_001982"
     CDS             complement(7467..8720)
                     /locus_tag="ACHA52_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8940669.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8749..9066)
                     /locus_tag="ACHA52_001983"
     CDS             complement(8749..9066)
                     /locus_tag="ACHA52_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8940670.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9092..9412)
                     /locus_tag="ACHA52_001984"
     CDS             complement(9092..9412)
                     /locus_tag="ACHA52_001984"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8940671.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9876..11291)
                     /locus_tag="ACHA52_001985"
     CDS             complement(9876..11291)
                     /locus_tag="ACHA52_001985"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8940672.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11308..12624)
                     /locus_tag="ACHA52_001986"
     CDS             complement(11308..12624)
                     /locus_tag="ACHA52_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8940673.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12774..13472)
                     /locus_tag="ACHA52_001987"
     CDS             complement(12774..13472)
                     /locus_tag="ACHA52_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8940674.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13761..14750)
                     /locus_tag="ACHA52_001988"
     CDS             complement(13761..14750)
                     /locus_tag="ACHA52_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8940675.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14830..15663)
                     /gene="msrA"
                     /locus_tag="ACHA52_001989"
     CDS             complement(14830..15663)
                     /gene="msrA"
                     /locus_tag="ACHA52_001989"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8940676.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15838..17472)
                     /locus_tag="ACHA52_001990"
     CDS             complement(15838..17472)
                     /locus_tag="ACHA52_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8940677.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17459..>19222)
                     /locus_tag="ACHA52_001991"
     CDS             complement(17459..>19222)
                     /locus_tag="ACHA52_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940678.1"
                     /translation="LTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLSTL
                     YSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTII
                     PAVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYR
                     TVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLC
                     SALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHS
                     PFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVG
                     VVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSW
                     LIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEA
                     IKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNG
                     KVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAP
                     NKLKDSRYVTKKEINKKNENK"
BASE COUNT         6104 a         3758 c         3193 g         6167 t
ORIGIN      
        1 tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat gctttttcag
       61 aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa
      121 aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata ttttaagaca
      181 gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa
      241 tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa aaaaagaatt
      301 tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga
      361 acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac
      421 ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt
      481 catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt ttaatgactg
      541 cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct
      601 tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt
      661 tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa
      721 agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc agcaagtgtg
      781 tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta
      841 tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc
      901 ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc
      961 ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact
     1021 agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac cgttcgaacg
     1081 tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa
     1141 attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc
     1201 cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt
     1261 tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt
     1321 cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt
     1381 cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca
     1441 gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata
     1501 tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca
     1561 aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc
     1621 atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt ttggacatct
     1681 acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc
     1741 gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa
     1801 acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc
     1861 tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg
     1921 attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca
     1981 agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc
     2041 ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa
     2101 tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga
     2161 tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc
     2221 ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa
     2281 aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa caaatattta
     2341 aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct
     2401 actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat
     2461 ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccatttt
     2521 tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc
     2581 ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt
     2641 tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt
     2701 tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat
     2761 catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc
     2821 gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg
     2881 cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga
     2941 attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt
     3001 ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg
     3061 gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat
     3121 tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat
     3181 tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg
     3241 cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact
     3301 gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt
     3361 gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc
     3421 aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa
     3481 aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca
     3541 aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa
     3601 ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc
     3661 gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg
     3721 aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa
     3781 acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac actgactcaa
     3841 gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt
     3901 acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt
     3961 gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac
     4021 aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac
     4081 catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat
     4141 tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatt
     4201 gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt
     4261 tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat
     4321 ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg
     4381 aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat
     4441 atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt
     4501 cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg
     4561 atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt
     4621 attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct
     4681 cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa
     4741 gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta
     4801 ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc tgccctgcat
     4861 caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttagactta
     4921 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag
     4981 tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac
     5041 ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt
     5101 ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca tctaacggat
     5161 acaactattt caatgattac cattacacta aaaaatcgga aacacacctc tccgaacaca
     5221 aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt
     5281 aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc
     5341 tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca
     5401 gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa
     5461 cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag
     5521 ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa
     5581 cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt
     5641 tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg
     5701 ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa
     5761 gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa
     5821 aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag
     5881 taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa
     5941 aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc
     6001 tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag
     6061 ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag
     6121 gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg
     6181 gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag
     6241 taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg
     6301 atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgcaa
     6361 tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag
     6421 aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt
     6481 tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa
     6541 atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc
     6601 gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga
     6661 ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct
     6721 atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga
     6781 acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat
     6841 gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg
     6901 agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata
     6961 aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt
     7021 tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa
     7081 cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt caatgacgcc
     7141 cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc
     7201 cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt
     7261 tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta atttgatcca
     7321 gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca
     7381 agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa cggaaaacaa
     7441 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
     7501 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
     7561 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
     7621 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
     7681 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
     7741 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
     7801 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
     7861 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
     7921 ataaacggtg cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
     7981 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
     8041 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
     8101 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
     8161 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
     8221 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
     8281 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
     8341 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
     8401 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
     8461 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
     8521 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
     8581 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
     8641 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
     8701 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
     8761 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
     8821 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
     8881 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
     8941 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
     9001 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
     9061 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
     9121 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
     9181 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
     9241 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
     9301 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
     9361 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
     9421 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
     9481 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
     9541 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
     9601 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
     9661 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
     9721 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
     9781 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
     9841 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
     9901 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
     9961 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    10021 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    10081 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    10141 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    10201 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    10261 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    10321 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    10381 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    10441 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    10501 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    10561 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    10621 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    10681 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    10741 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    10801 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    10861 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    10921 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    10981 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    11041 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    11101 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    11161 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    11221 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    11281 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    11341 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    11401 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    11461 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    11521 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    11581 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    11641 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    11701 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    11761 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    11821 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    11881 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    11941 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    12001 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    12061 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    12121 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    12181 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    12241 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    12301 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    12361 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    12421 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    12481 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    12541 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    12601 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    12661 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    12721 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    12781 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    12841 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    12901 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    12961 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    13021 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    13081 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    13141 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    13201 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    13261 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    13321 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    13381 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    13441 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    13501 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    13561 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
    13621 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
    13681 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
    13741 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
    13801 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
    13861 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
    13921 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
    13981 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
    14041 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
    14101 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
    14161 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
    14221 attttttttg tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt
    14281 cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga
    14341 tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg gtcacaactt
    14401 gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct
    14461 gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc
    14521 ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg
    14581 gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa
    14641 tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc tgaatgaccg
    14701 gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa tttttctcct
    14761 cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact acattttaaa
    14821 gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt tttttactcg
    14881 ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt tcggttgttt
    14941 tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg gttcgatttt
    15001 tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg attgttccgc
    15061 taattctttt tgtcgttcga cttgataaaa aatcactgga cgatattgct tccctctatc
    15121 ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg tcagcaactc
    15181 gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat gacctgtgta
    15241 accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa
    15301 aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga aacatccccc
    15361 tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg aaaagtcata
    15421 tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta catcaggcgt
    15481 cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt
    15541 aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc gttcccaatc
    15601 tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt gttcctcatc
    15661 catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata
    15721 aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa aaaagagaga
    15781 atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa agttaattta
    15841 ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg taacaagata
    15901 agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa ctcctttgac
    15961 tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt gatgatttat
    16021 ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc tcttctcatt
    16081 agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta cccattgact
    16141 gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag cgataggaga
    16201 ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg tttctttgtt
    16261 tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt tactagaaac
    16321 agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc ttgtcttatt
    16381 gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata ttatttgcaa
    16441 gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca gaagatacca
    16501 tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa tctttttttc
    16561 tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca gaccaactag
    16621 tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat cgaacacatt
    16681 agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt agagcatttg
    16741 ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg ccgcactaaa
    16801 taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa ccaaacgaat
    16861 aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat ggctgtacac
    16921 tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt ttccagagat
    16981 gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag cttcggcaat
    17041 tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga gacaactaaa
    17101 taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag ctacgagtgt
    17161 tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat tttttgtcat
    17221 ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat ttacaccata
    17281 tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg ataacccctt
    17341 gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg tatttttgaa
    17401 aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta ctactttttt
    17461 atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa tctttcaact
    17521 tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc agaatactga
    17581 tccataaagc catgagagca gttggagtat attttgttgt aatttctatc ttattttctc
    17641 cagcatttac atccacattc actaagccat gttttttata agagtcattt acgactttac
    17701 cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact ttttgctttt
    17761 tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc cctttttccc
    17821 acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt atcgcttctg
    17881 ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg agtgtttctt
    17941 cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt tcttttgctg
    18001 ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat gaatattttt
    18061 tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga
    18121 tacctaagat acgccaagga aactggagta agcttatcgg tgttttttga aacaaaaacc
    18181 atggaaataa attagttacg catacccatg tagaaactac gccaacatag atgatttttt
    18241 cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc aatcctaata
    18301 accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta tctgaaatag
    18361 aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga taattcatct
    18421 gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa acagccttta
    18481 ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa aacaaacgaa
    18541 ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca tgagtataca
    18601 caagtagact tatactgaca gataaagcga tgactttttt ttctctattg aatatcaatc
    18661 cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct gcaattgcgg
    18721 atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa ctaaataaaa
    18781 aagcagcatc tatcttttta aaaaactttt ctccataata aaaactaatc agaatcgtac
    18841 aaatatttaa taaccaaata tatagaacat aacttaatat taagttattt gaaattccat
    18901 aaaaaacgac tgcaggaatt attgtaagaa aaggataaaa atagttcagt ccatctccat
    18961 aattgttaaa tgttgtatag ttgattggcc cttcaaaaat agaagataac cctttgattc
    19021 gtgaaagatg aaaagataaa tcacctgttg tataaatttc ttgagcattt ctcataaaat
    19081 ttactgaata taaagtacta agtaagataa gaactactaa aagaaatgct tttttactta
    19141 ttttcataga tttgttgttc tcctttttta attctatttt cagtatacta gaattgtctt
    19201 tttttaataa gttttgcgtt aa
//
LOCUS       DAYYGY010000060        18295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000060 DAYYGY010000000
VERSION     DAYYGY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.061"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(377..1960)
                     /gene="abc-f"
                     /locus_tag="ACHA52_001992"
     CDS             complement(377..1960)
                     /gene="abc-f"
                     /locus_tag="ACHA52_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8940679.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2065..2193)
                     /locus_tag="ACHA52_001993"
     CDS             complement(2065..2193)
                     /locus_tag="ACHA52_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940680.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2538..2682)
                     /locus_tag="ACHA52_001994"
                     /pseudo
     CDS             complement(2538..2682)
                     /locus_tag="ACHA52_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            complement(2905..3384)
                     /locus_tag="ACHA52_001995"
     CDS             complement(2905..3384)
                     /locus_tag="ACHA52_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8940681.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3457..3741)
                     /locus_tag="ACHA52_001996"
     CDS             complement(3457..3741)
                     /locus_tag="ACHA52_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940682.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3819..4013)
                     /gene="ssrS"
                     /locus_tag="ACHA52_001997"
     ncRNA           complement(3819..4013)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA52_001997"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4085..5371)
                     /locus_tag="ACHA52_001998"
     CDS             complement(4085..5371)
                     /locus_tag="ACHA52_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8940683.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5509..6180
                     /locus_tag="ACHA52_001999"
     CDS             5509..6180
                     /locus_tag="ACHA52_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8940684.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6146..6634
                     /locus_tag="ACHA52_002000"
     CDS             6146..6634
                     /locus_tag="ACHA52_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8940685.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6647..7594
                     /gene="prmA"
                     /locus_tag="ACHA52_002001"
     CDS             6647..7594
                     /gene="prmA"
                     /locus_tag="ACHA52_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8940686.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7595..8359
                     /locus_tag="ACHA52_002002"
     CDS             7595..8359
                     /locus_tag="ACHA52_002002"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8940687.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8592..10805
                     /locus_tag="ACHA52_002003"
     CDS             8592..10805
                     /locus_tag="ACHA52_002003"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8940688.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10816..11262
                     /gene="dtd"
                     /locus_tag="ACHA52_002004"
     CDS             10816..11262
                     /gene="dtd"
                     /locus_tag="ACHA52_002004"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8940689.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11433..12692
                     /locus_tag="ACHA52_002005"
     CDS             11433..12692
                     /locus_tag="ACHA52_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8940690.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12781..13728)
                     /locus_tag="ACHA52_002006"
     CDS             complement(12781..13728)
                     /locus_tag="ACHA52_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8940691.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14122..15414
                     /gene="hisS"
                     /locus_tag="ACHA52_002007"
     CDS             14122..15414
                     /gene="hisS"
                     /locus_tag="ACHA52_002007"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8940692.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15429..17198
                     /gene="aspS"
                     /locus_tag="ACHA52_002008"
     CDS             15429..17198
                     /gene="aspS"
                     /locus_tag="ACHA52_002008"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8940693.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17307..18182
                     /locus_tag="ACHA52_002009"
     CDS             17307..18182
                     /locus_tag="ACHA52_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8940694.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         5777 a         3397 c         3898 g         5223 t
ORIGIN      
        1 tttttttgcg gagaagaccg caaaatggta atttaacttc aatttccctt cttttttaca
       61 ggatcttctt agggaatttt gcttggaaaa gtggtcaaaa gggaaaagac tggcatttta
      121 tttattgatt ttcaggaaaa tcccaatatt ttattttcat tcatctttat aagggacatt
      181 acatatgtaa aaagaaaaaa catttttaga tcagcctcgt gggctgtcga ctttattctt
      241 cgctttatgt ttagctagaa aaccgatccg aaacattcat tgatcgaata ataaaaactt
      301 gattttccac ctgtactttg cctgaatcgt gaaagcacag gtatgaaaat caagtttttt
      361 gttgcgaaaa aacgtcctag tcaatcagtt ggccgagcgg ctggcggctg atctgctcct
      421 gatcaataac gagtgcttct tggaagtgtt gctgcaaaaa gtagcggtca tggctgactg
      481 ctaggagcgt tccttgatag tcttgcagca tttcttcaat ttcttctcga gcatagctgt
      541 cgaggtgatt ggttggttca tccaaaatca ggacgttgat tttttctaaa aagagtttca
      601 gcaacgccaa acggattttt tcaccaccgg ataaatcttt gatccgttta cccacatcgc
      661 tttgataaaa gccacttttg gctaattgcc gtcgagcgac ttcttcgttt ccagtcaatg
      721 tgatggcgta agccagcaaa cggctatcgg gatcggcaaa ctgcagctgc tgcggcaaat
      781 aaccgatttg caagctacta cctaatttga tggtgccacc gtaaggcttt tcctcaccga
      841 gtaataattt gagtaaggtt gattttcctg caccattgtc gccgatgatg gctaaatgat
      901 ctccacgata gactgcaaaa ctgctgtctt tgaatagtac ttgatcagcg tactgctggt
      961 gcaagttttg aatgatgaaa acttctttgc cagattttcc accattagac aagctttgca
     1021 gccgattttt cgggggaggt ggcggtttga cgagtgtcag tttcgccagg cggcgttcca
     1081 gttctttggc ttttttgaag aaggactcat tgtctccttc atgtgcccac tgacgaaaac
     1141 gacgaatcat gactttcaac cgctggactt ctttttgctg cagttcgtaa ttttttgtca
     1201 gctcagccaa gcgagcttct ttcaagactc gaaaacggct gtagtttccg ggatactcaa
     1261 tcaatgcacc ttcttccagt tcccatgttt ttgtggtgac ttgatcgaga aattgccgat
     1321 catgagaaac gaccagaacc gatttttttg tattctgtaa atagttttcc aaccagcgaa
     1381 tactgttaag atctaaatga ttggtcggtt catcgagtaa aagtaaatcg gcatcggctg
     1441 ctaaaatttt ggctaaagcg actttaactc gttcgcctcc gctaagttct gttaaccgtg
     1501 cttcctgacg gtgtggaatc cctaagcctt gcaagatcgc tgcaatccgg tcttctaata
     1561 cgtaaccgcc tagttcttca aagcgatgct gcagctcacc gtaacgggcc agacacttct
     1621 ctaagtttgt atctggttct gtcatcaatg cttcgtagcg actcatttgc tgtttgaggt
     1681 cctgtatctc agcggcagaa tggagcaagt agtggttgac ggtcgcttcg gaaactgtaa
     1741 gttcttgttc gaccgtcccg atctttaagc cttttttgcg actgataaca ccgctgtcga
     1801 caccttctct gccggtcaag atttgtaaaa aggttgattt gccagttccg ttgataccga
     1861 ctaaggcaat tttttcacca gcttcaattt gacaggataa atcctcaaat aatggattcc
     1921 cgccgattgt ttttttaata gaaattgctt gtatgatcat gatctctctc cttgagacca
     1981 ctcaaaaagg ccatggttat ccatggcctg tgttatttaa actcagatta tcatgagaaa
     2041 aataccttga gatggccatt gattttacgt gaattcaact gttttggaca aacttgtccc
     2101 gtcaacagca actgtaaaac caattgcatg gctaaatgcg atatggtatc gttggtaatt
     2161 ttcattactt gttattttag tcatgcgcat catagtattt tccctccttc gtgtagttgt
     2221 cttttccagt gtacctgaaa gtgtcagaaa gttcaactag cgctgacatt tacttaatat
     2281 ttatcgatgt gtaattctgt cagagagctt tttttatctg actgagggta atgtctcgtg
     2341 gaccacatca ttgtattctg agctaaacca gcgttcaggc gaaatcatat ggtgtttgat
     2401 aaaaaacttt gtctacatga cttccttttg ccatttgctt aataccgttg cattaaacag
     2461 ctcttctctt tggaaatctc accaacatcg cgttcaggag tatcgttgtg gttgcaatgg
     2521 caagcggtag cctttcatca attcttgaat gatttaccga atgtagcact tgccttctcg
     2581 cggtacccga acccgaaata aaattttagt ttggcgataa aatcggtatc cgccatcgat
     2641 acttcatatt tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc
     2701 tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact
     2761 tttttttatt ttacttgaac tcattgactg ttttcccttt attttaaaca ttataaagac
     2821 cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt tttgtgcaaa aaaaactgcc
     2881 gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg tctttagctg gcaattggtt
     2941 ttctaaatca gttcggacaa caagcacatc gcaagctgcg tttctaataa catattcaga
     3001 aacagaacca ataaataaac gttctacagc attcaatcct gtggctccta acataatcaa
     3061 atctacttga ttatcttctg ggatttctct ggcaataatt tgttttggtg agccatattc
     3121 tacgacagag gttacattat tcaagccagc tttcttcgca ttgctttcat aatctgccaa
     3181 tgtttgcttt gccatttcag tagcttgttc tgcaagcatt ccatcaaagg atgaaacggt
     3241 ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt tctccgttat ttcggatagc
     3301 tacattgacc gcctttttaa aagctaattc agcttcatca gaaccatcta cggcaaccat
     3361 gatttttttg tattgttgta acataagcat tcactcctca agtcaatcgt attgtactct
     3421 tttattgtag aacactttag ggaaaaagag aattatttac tgctttggtt cagcaatttt
     3481 ttttgcaaat cggatactga aggtggcaga aacaataatt acgatcagta aaaagaccaa
     3541 ggcaataaag cgttgattga aaaaagctgt taaaataccc attacgccta aaacagcata
     3601 tgcagctccg taaaattgta ggaatccttg atttttatca tttttgttga tcaatacaaa
     3661 aaagatatcc tgtttattaa ataaataata gctaacgaat aataatacta aagtggcgat
     3721 aatcattaaa atcctaatca tgaacaaatc ctccaaaata agcaacaaat agaaaaagca
     3781 atttgccaag agcaaattgc tttattcttt aaaagatggt aatctccgtt aatgccgttg
     3841 tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg gtcccatggt cgtagcttcg
     3901 aactaccaaa tgataaggca tgttaccagc agtgacacaa gacgggtccc aagatatcaa
     3961 taaaaatgtt cggtcaacac acaaagagga caattcgaac atcacagatt tcccacctct
     4021 atagtagcat atgaatttaa aaagagcaat gagagagctc gcagaaacag aaccgttttc
     4081 ttgtctagtt ttttggagga tgctttttcc actcttttat acggtaatac tgttgaccca
     4141 gagcctgttc atatttccct gtatcttttg gctgataata ctgagcattc ttcagtttat
     4201 ctggtaaata ttgctgattg acccaagcct gatcaaaatg atgaggatac tgataaccca
     4261 cgccacggtt gagtgatttc gcacctttgt aatggctatc tctcaaatga tcaggaacat
     4321 ctccagcttt gccctctcga atatcagcaa gtgctgcatc taatgccatg taggcagaat
     4381 tggattttgg cgacaagcaa agatcgacga cgacgtctgc tagtgggata cgtgcctcag
     4441 gcaatcccaa tttttcagca gctaatacag cgttcactgt acgagccgca gcagcaggat
     4501 tacctaaacc tatatcttca taaccgataa ccatcagacg ccggcaaata cttgccagat
     4561 cacctgcctc aactaaacgg gcgagataat gaagtgcagc atccacatca cttccacgaa
     4621 tcgatttttg aaaagccgaa ataacgtcat aatgagcatc tccatttttg tcatgggtaa
     4681 gagcttttct ttggacacat tcttcaatga tcgacagtgt aagatgaatc cttccttctt
     4741 tatctgaagg agtcgatagt gtagccagtt ccagcccatt caatgcactc cgcaaatcgc
     4801 cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc taactggatt gcttgttgtc
     4861 caaggccgcg ttctttatcc cttaaagcat gttcgactgc caactggatg tcttgttctg
     4921 ttaatggttt gacttcaaag atctgtgtac ggctgcggat cgctggattg atggtaatat
     4981 aaggattctc agtagtcgct ccgatcaaga tgattcggcc actttctaaa tgaggaagca
     5041 gaaaatcctg ttttgtttta tctaatcggt gtacttcatc caataagagg atcactgtac
     5101 cactcatttt tgcttcttcc gctacaactt gtagatcctt ttttgaatct gtagcggcgt
     5161 ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc acttgcgata ctcgtttttc
     5221 cagtaccagg aggtccatat agaatcatgg aagagagcat gcgagcatcg accatgcggc
     5281 gtatgatttt tccttccccg actagatgct gctgaccaac gacttcatcg atcgttcgag
     5341 gtctcatgcg ataagccaat ggctgttgca taggattttc ctccttcttg tttttacgtc
     5401 ccttaattat atcatattta gcaagatgcc tagaaagaac agccttctgt tgttttgttt
     5461 tcatagctta tccaactaat attgtgtatg ataaagtagg tgagaaaaat gacggataaa
     5521 caaaagatag atgaattttt ttggaataaa acgacgccag aagtggcacg ggatttattg
     5581 gggatgtatt tggagcatga cacaccagaa ggaagattag ctggatatat tgtagacgcg
     5641 gaagcatacc taggtccaga agacgaggcc gcccatagct atggtttaag aagaacaccg
     5701 cgtgtgcggg caatgtatga aaaaccaggt acgatctacc tttacacgat gcatactcat
     5761 cggattttga acatcattac tcagccagaa gggattcctc aaggagtaat gatacgtgca
     5821 atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc gtggcggaaa aactggtcca
     5881 gatatcagca acggtccggg aaaattagtg gaagcattag caattcctca agaactttat
     5941 ggtcagtcta tagcagatag ttcgttacgc ttagtttttg agaaaaaaaa gacaccaaaa
     6001 aagatcaatg ctttgcctag aatcggtatc ccaaacaaag gtgtatggac agaaaagccg
     6061 ttgcgttttg ttgtgtcggg aaatccttat atctctttac aaagaaaaaa ccaaatcgag
     6121 aaagattggg gttggaggaa agaaaatgaa aaagaaggat ctattaacat atttagatga
     6181 aataattgaa aaacaagtga cagactatga tgtagcttta gattgggata caaaaaatca
     6241 tacgatcgag atcgttgttc gcttgtttgc agaaaataaa gcacaatttg agattgatga
     6301 tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt gaagacggcg tacttctttt
     6361 taatcctcag aaatctgttt ttgatgaacg caattatcta gcagtaatcc cttatgaagg
     6421 aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg gaatatctaa atgaagtatt
     6481 agctcaaggg caaagcgatt tgcttgattt tttggatgaa gacgatgacg aagctgtatt
     6541 cgagcttcac tgggataatc agcaactagc agaattagta gaagtaaaac agcaaaatga
     6601 agcagatact tatttaccgt acccaagtta ttaaggagtg acagatatga aatggaatga
     6661 agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt gctaatattt tgatggaggc
     6721 aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt gaacattttc agtcggatca
     6781 atatggtgag attttagata aaaatgattt tacatcgctg aaagaaggcg cactagtcat
     6841 ggcttatttt ccggaaacga ctttcttacc ggaaatcttg ccttttatca aagatcgtat
     6901 cacacaatta cctgagtttg gtctagctat tggaaaaaat aaagtaacag ttagtgaagt
     6961 agaggaaagc aactgggcaa cagcctggaa gaaatactat catccggtgc gtattacgcg
     7021 aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca gaagatcctc tagaaaaaat
     7081 tatcacacta gatcctggta tggcatttgg cacaggcact catcccacga cttccttgac
     7141 attgacagca ctagaaacag tcttgcgtgg tggtgaaaca ttgctagatg tgggaactgg
     7201 gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca aaagatgtct atgcctttga
     7261 tttagacgaa gtagccgttc gttcggcgaa agaaaatatg gatatgaatg aaatagccaa
     7321 agatgtccat gtatcagcta acgatctttt gacaggaatc gagattgaaa gtgatgtaat
     7381 cgttgcgaac attttagcag atatcatctt attgatgatc ccagatgctt ggcgtttact
     7441 gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa gagaagaagc aaatggttct
     7501 ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt ttccagcaaa aagactggta
     7561 tgcaattatt ttgaaaaaac cagaggtgga atagatgcag cggtattttt taaaagaatc
     7621 atatgaatca aaagactatt ttgttttaga aggggaaaac tatcaccaca ttgtacgggt
     7681 tatgaggatg gaacccgagg atgaagtttt tttggcattc aatgatcaaa tagccattgt
     7741 tgcagaaatc acggagatta ctgaagaggc tgtttatctg aaagaaatta aaaaagaaga
     7801 aaatcaaaag gaactgccaa gttcgattac catagcgagt ggctatccta aaggggaaaa
     7861 gctggattgg atcgttcaga aatccactga attaggagcg caagcttttc ttggtttccc
     7921 ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt atgaaacgac aacaacggct
     7981 tgaaaagatt gctcaagagg cagcggaaca atcccatcgg cagatcgtgc catcagtaga
     8041 gttattggaa acgaaacaag atttatttga gcggctagat gattatgacg tggtcttggt
     8101 tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat gcagccaaaa ttcttagtga
     8161 aacaaaacca gatagtcgaa tcttagctgt ctttggacca gaaggtggat tatcccgcga
     8221 tgaagtcgat tattttgtag aacacaaagg gatactttgt ggattaggac caagaatcct
     8281 tcgtacagaa acagcacctc tttatttatt aagtgctgct agctatcaat gggaattaat
     8341 gaatccttct ttcagctaac gttcttcatt aataaaagag tatcgacaga aagaattgaa
     8401 aaaatagtca acaatttgta taatagaaac aattatttat acaatggata cgaaaaatcg
     8461 tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa acgtataact gcacggattt
     8521 tgggacaatt atcaaacaga tatttgtccc gattcccgtt ttaccgaaaa aagaaagggg
     8581 gatcgattcc tgtgacaaaa gaagaaattt taacgggacc cggtgtcata aagctcgtta
     8641 gtcaatacat gggtcccgaa catgtagcat ttgttgagaa agcatgcgaa tacgcgactg
     8701 ctgcacatga cggacagttt agaaaatcag gcgaacccta cattattcat cctatccaag
     8761 tcgcaggtat attagcagat ttaaaaatgg atccccatac agtggctaca ggcttcttac
     8821 atgatgttgt tgaagataca gaaatcactt tagaagatct gagagaagaa tttggcgatg
     8881 acgttgctat gttagtggac ggcgtaacca aattagggaa aataaaatat aaatcccacg
     8941 aagagcagct agcagaaaac caccgaaaga tgctgctagc aatggctcaa gatttacgag
     9001 ttatcatggt caaattagct gatagactac ataacatgcg tacgttgaag cacctgcgag
     9061 aagataagca aagaaggatc gctcaggaaa ctttagaaat ctatgcgcct cttgcacatc
     9121 gtctagggat cagccggatc aaatgggaat tagaagatac agcacttcgt tatctaaatc
     9181 caaaacagta ttaccgtatc gtccacttga tgcagacgaa gagagaagaa cgtgaaaaat
     9241 acgtaagtgg tactgttgaa gatattcgaa tagcgacaga agagctgggg atttttgcag
     9301 aaatctatgg acggccaaaa cacatttatt caatctatcg taaaatgaaa gatcagaaaa
     9361 agcagttcaa cgaaatttat gacttgctag ctattcgagt gatcgtagat tcaatcaagg
     9421 attgttatgc tgtattagga gcaatccaca caaaatggaa accaatgccc ggcagattta
     9481 aggattatat tgccatgcca aaagctaata tgtaccaatc tttgcatact accgtgatcg
     9541 gtccggcagg aaatccggta gaaattcaaa tccgaacaca ggaaatgcat gaaatcgctg
     9601 aattcggggt tgctgcacac tgggcctata aagaaggaaa aaatgaaaaa gtagaaccag
     9661 atggtatgac gaaacaatta agctggttcc atgagatact cgaacttcaa gacgaaagct
     9721 atgatgcttc tgaatttatg gaaggcgtaa aaggagatat ctttagtgat aaagtctacg
     9781 tcttcacacc aaaaggagac gttactgagt taccaaaagg atccggacca ttggactttg
     9841 catacagtat ccatacagat atcggtaaca aaaccactgg tgcaaaagta aatggcaaaa
     9901 tggtgcagct tgattacaaa ttgaaaaacg gagatatcat tgagatcatg acttctccaa
     9961 attcatttgg cccaagtcgc gactggttga aattagttgc tactagcaaa gcaagaaata
    10021 agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa tgtgatcaaa ggccacgaat
    10081 ccgtggtcaa atgtattaca gatctaggat ttacgcctaa agatattttg acgaagaaca
    10141 aactgcaaga agcactcgat cgttttaatt atcaaacaga agatgatctc tatgcagctg
    10201 tagggtatgg agaagttagc cccttgacga tggccaatcg tctgactgaa aaagaacgta
    10261 aagaacaaaa aatcgagcag caaaagcaag aagcagaaga aatcatgaat cagccgaaaa
    10321 aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt tgtcattcaa ggggtagaaa
    10381 acttactaat tcgtatcagt cgctgctgta atccgattcc tggtgatgat atcgttggtt
    10441 atatcactaa aggcagaggg atatccattc atcgtcgaga ttgtccgaat gttcagcctg
    10501 acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg ggaagataca tcgaatacac
    10561 gaaaagagta tgatgcagat ttggaaattt acggctataa tcgttcaggc ttattgaatg
    10621 atgtacttca aacagtcaat gcgctaacga aaaatctcaa cagcgttgaa gcacggacga
    10681 ataaagataa aatggcgacg atccatttga cggttggtat ccagaattta tcccacctaa
    10741 agagtatcgt ggataaaatc aaagcagtac ctgatgtcta cagtgtacgc cggacgaatg
    10801 gataggagga cagatatgag agcagttgtt caacgggtaa gtaaagctgc tgtgtcagtc
    10861 gatcagcaag aaattggaaa aattggctac ggcctacttg ttttattagg cattcatgaa
    10921 accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa ttgcacaaat gcgtattttt
    10981 gaagatgaac aaggaaaaat gaatattagt gtagaagatg tgggaggaca aattttaagt
    11041 atctctcaat tcacactgtt tgcggatacg aaaaaaggaa atcgaccaag ctttgtggaa
    11101 gccgcacgac cagaaacagc tattccttta tacgaatcat tcaatgaagg aatcagaagt
    11161 cgcggcatca cagtcgctac cggacaattc ggagcagata tgtcggtttc tctcgtcaat
    11221 gatggacctg taacgatcat tattgattct aaaaacaaat aaagtaatct ggttttcact
    11281 agtaaaaact atgaaacacg tcgcttaagt tagactgata aactctaact taagtggctt
    11341 ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa aaaaagttaa gcttaaatta
    11401 tacaagttat tttttatagg aggttgctga agatgaaaga agtaccagta gtagatatca
    11461 aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa catgttggag gagttacgcc
    11521 atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa taaagaagct atcacgatct
    11581 acggatcatc agcagcaaaa gtattttatg atccaaggaa tttcaaacga aaaggtgcta
    11641 tgccaaaatt agtattgaag acactttttg gtcaaggtgg agtacagact ttggatggag
    11701 ctgctcatca ccatcggaaa aatattttta tggatctaat gacaccggaa agaatggaag
    11761 attatcatcg tatcttagat aagaatctga cacaagcact tgaagcacag catggtcaat
    11821 ttgaactatt tgatttaagt aagatggttt tctttacatc tatttgcgag tgggcaggta
    11881 tcaatttatc agcgatttct aaagatgagg tcgaaaaatt agcagaatat caaatctcta
    11941 tgatcagcgg aacgtttact tctccgattg atcacataaa aggtgtcgaa aatcgcaaaa
    12001 aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg acaaaatcct gtggctggaa
    12061 aagaaaatgt ggcattatat gcttttgcca atgccacaga tttagatggt cagcttttac
    12121 ctctggaagt ggcagctgtt gaattattga acatcatccg tccaacggtt gctctaactg
    12181 tgtgggcggc attgatggga catgcacttt tcagtagacc agatttgtat cagcaattaa
    12241 aaaatgactt ttcgacgtta caagatccgt ttattcaaga aatgagacgc tattatcctt
    12301 tcttcccaat gctacctgcg atttcgctga aagaagtaga agtagatggt tatcgtatac
    12361 cagaaggcag ttgggttatt ttagatttat atggaacaga ccatgatgaa cgaacagttg
    12421 aagcgccaga ttcctttatg atcaaacggt atgtagggaa agcaaaagat atttcctata
    12481 aagaagaata tgagatgatt gcacaaggcg ggggaaattt tcgacaaatg catcgctgtg
    12541 caggagaatg gatcactctt catagtctac gtgtgttttc cgatcagcta gtaaataagt
    12601 ttgagttctc tgtacctgaa caagattgga cgattccatt caatcagttt ccgacttatc
    12661 cgaatagccg agcgctactt tacaagaatt agattagcat aaacttatcg aaaaaaagga
    12721 agatcaaaca gtgctgatct tccttttttc atattactat aaattgataa ggtcaattga
    12781 tcaaaatcca cgaaatgctt catcaatggt ttgataatct tcattggata aatgcaaatc
    12841 taaagcttgg acattgtcag atacttgctc tggacgctta gctccaggta taactacaga
    12901 cactcgtgga ttcttcatgt accaagctaa aacaagttgc gcaggagtgg cttggtatct
    12961 tttagcaatg gggcgaagta catcgacagc ggttaaaatt tctctgaaac gttctccttg
    13021 gaaatctgga tttctttttc gaggatctcc ttcaccaaat tgtttttcct caccaagttc
    13081 atattttcct gtcagcaaac cggaagcaag aggaaaatag ggaacaaaac tgattttgtt
    13141 tttttcaaga taagggaata attctttttc agcctgccga tggatcaaac tgtatttatc
    13201 ttctactaca tcgacatatc catctgcatt cgcttctttc aattgttcta acgtgaagtt
    13261 tgacacaccg actgctcgga tttttcctgc ttcttttaat tcgtgcaatg tagcaactga
    13321 ttcatttttt ggtgtgcttt catccggaaa gtggatataa aaaatgtcga tgtaatcagt
    13381 ttgcagacgt tttagtgcat cttccaccga ttgtttgaga aactcaggag aattatcaaa
    13441 tgttcgccct ttatttggta catgagcagc ttttgtagcg atgacgacac gtgagcgatc
    13501 atattctttt tccttcagta cctcgccaat cagttcttcg gaacggccgt ttccgtaagc
    13561 ataagcggta tcgatcaaat tgatcccgct attcaaagct gtacgaacta cttgttttcc
    13621 tgtctcatct tccaaaccgt caaaaagatt atgtcctcca acggcatttg ctcctaatcc
    13681 aagctgttct gcgtagactt gtgaatgtcc gattcttatt tctgccatag ctgattcctc
    13741 ctttttgtct agtctaaaac aaaataccaa aaagctgaac taaaacgttt ttgtgagggg
    13801 aaatagtcag atatctcttg actttgagac tggtttttag taggttataa gtaatatcga
    13861 atgtcaatga aagagcgagt aagtttttga aactttacaa gagaggatca tcgaggctga
    13921 aaatgatcta aagtttcatt agcctaaaga cactctggag atggacgagt gaaaagccgt
    13981 cccgcgtacg agcgttattc gtaagaggaa agagaaatct ttcaaaatta ggtggtaccg
    14041 tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg tgattttttt atttacatag
    14101 ctaagaaagg gagagaaaac tatgagtttc caaagaccaa aaggaacaaa tgatattttg
    14161 ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag cacgtttatt attcagagat
    14221 tatcagtata atgaaatgcg tacaccgatt tttgaacact atgaagtgat atcccgaagt
    14281 gtaggggata caacggatat cgtttcaaaa gagatgtatg atttttatga caaaggggaa
    14341 cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg ttcgttcttt tgttgagcat
    14401 aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt tttatatggg accaatgttt
    14461 cgctacgaac gccctcaaaa aggacggtta cgtcaattcc atcaaatcgg agtagaagca
    14521 tttggatcag aaaaccctgc aactgatgtg gagagcatgg tgatggcact tgatttcttc
    14581 aaacagttgg ggatcacgca aatccggcta gtcatcaatt cattaggtga taaagaaacg
    14641 cgtcaagcct atcgtcaagc attgatcgac tatcttttac cttttgaatc agagctgagt
    14701 gaagactcta aacgccgcct tcatgaaaat ccattacgcg tattggacag taaagacaaa
    14761 cgagatcaaa aatttgtggc agaagctcca tctatcttag attatctaag cgagcctgct
    14821 caaaaacatt ttgataccgt tgtttccatg ctggatgcat tggctgttcc ttatgaaatc
    14881 gacagtaata tggttcgcgg actagattat tacacacata cgatttttga aatcatgagt
    14941 gacgcaccaa aaatgggggc acaagcaacg atttgcgcag gcggacgtta tgacaatcta
    15001 gtggaagaac taggtggtcc ttcaacacct ggcttcggat ttgcaatggg tatcgagcga
    15061 ttactcttga ctatggaggc agaggaagta gttatccctg catttaatga gttggatgct
    15121 tacgtggtcg cactcggaga cgaaacaaat atcgaagcat tgaaagtggt ccaagcgatt
    15181 cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc gtaaagcaaa agcgcaattt
    15241 aagacagcag ataaagcgaa tgccaaatta gtattggtga tcggtgaaga tgagcttgct
    15301 agtcaaacga ttaatgtcaa agcaatggca agccgaaaag aaaaaagttt tccattagaa
    15361 gagatatatg aacattttga tgaagtctat gatgaaatga ctttattcga gtaggaggaa
    15421 aaaaataaat ggcacaaaga acgatctatt gcggattagt ttccgaacaa caattagaac
    15481 aaacagttac attaaaagga tgggtacaaa aaagacgaga ccttggggga gtaatcttta
    15541 tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt taatcctgaa atcaacaaag
    15601 aagcatggga aatcgcggat aaatgtcgca gtgaatacgt gatcgaagtg actggggaag
    15661 ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc cactggtgaa ttcgaagtga
    15721 ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc gccatttcaa attgaagatg
    15781 atcaagcaat cagtgacgaa atacgtttga aatatcgtta tttggatctg cgtcgtccaa
    15841 aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa agcgattcgt cattatttag
    15901 atgataatga ctttctcgat atcgaaacac cttacctagg gaaatctaca ccagaaggcg
    15961 caagagacta cctagttcct tcacgtgtcc atgctggaca tttctatgcg ttgccgcaat
    16021 ctcctcaatt gtttaaacaa ttgctgatga atgctggatt cgaccgttat tatcaaatcg
    16081 ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca gcctgaattt acgcaagtag
    16141 atattgaaac aacgttctta acagcagaag aaatccaaac ttacacagag ggattgattg
    16201 cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt gccatttcct cgtatgacct
    16261 atgatgaagc aatggctcgc tacggcagcg acaaaccgga tacacgtttt gatatggaat
    16321 tgatcgatct aagcgataca gtcaaagaag tagaattcaa agtcttccaa atggctcttg
    16381 aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc tgcagatcgc tactcacgaa
    16441 aagacatgga tcagttaggt caatatgtag gacaattcgg agctaagggg cttgcatggc
    16501 tgaaagtaga agaagacgga ttgaaaggtc caattgctaa attcatggga gaagcaacag
    16561 aagcaatcat taaagcaaca gatgcaaaac caggtgactt gctgatgttt ggcgcagata
    16621 aaagtgaaat cgtcgctgct gcattaggtg ctatccggac acgtttagga aaagaattag
    16681 gactaattga tgaatcgaaa ttcaatttct tatgggtaac tgattggcca caatttgaat
    16741 tttcagaaga agaaggtcga tatgtctctg ctcaccatcc attcacgatg ccaaaagaag
    16801 aagatatccc gttattggct gaagaccctg caaaagtcta tgcagaagct tatgatatcg
    16861 tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat tcatactcgt gagcttcaag
    16921 aaaaagtact agaaactcta ggctttacaa aagaacaaat ggaagaacaa tttggtttct
    16981 tgttaacagc tttagattac ggattccctc cacatggcgg tattgcatta ggattggacc
    17041 gttttgcaat gttattagcc ggagaagata atattcgtga agtcattgcc tttcctaaaa
    17101 acggaaaagc agcagatgtg atgagtgatg caccaagtat ggtttcgcct ttacaattgt
    17161 tcgaattgaa tattgatgta acgaatatag aagaatagcc gataaaaagg taaaaaggat
    17221 ttgtcgaata cgcttctttt gacaaccttg atttaattga aaaacacttt ataatgaagc
    17281 cataacatat aaagcggggc ttttccatga aaaaaacatt cgaaaattgg gcttatcatg
    17341 attacacgac caagctttcg gtatctatcg tttatgcgtt gatcgcatca gtcgcattga
    17401 atttctttta tcagccagga catatttatt caagtggtat tactggtcta gctcagattt
    17461 tgacgacttt gtcaaaatca ctgataggat tcgaagtacc ggtatcaaca actttgtatt
    17521 tactgaatat acctttgttt gtattggcgt ggttaaaaat cgggaagaag tttacaattt
    17581 tcacttttat cacagtcact ttgacctccc tttgtatcca tattttaccg gaaacagtcc
    17641 tatctaccga tccaatcatc tgtgcgttgt ttgggggggc tgtgatggga ctaggtattg
    17701 ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga tatcgtgagt atcacgattc
    17761 gtaaaaaaac aggaaagtcg attggttcta tttcgatcta cttcaatgca ttgatcgttt
    17821 ttgttgccgg aattctattt ggctggcaat atatgtttta cagtacgttg tctattttcg
    17881 tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg
    17941 taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca
    18001 cgatcatcaa tgaagcagaa ggtgcttata agcatgataa gcagacagta ttgatgacga
    18061 tcgttacacg atacgaactg cctctactta gagaagcaat gaaagcttct gatccacaag
    18121 cttttgtaag tattgcagaa aacgtccaga tacttggtcg tttctatgag gatggcattt
    18181 agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga cagacgttcg tttgtctgca
    18241 gtttttttct accatagtag aatcagcaaa gaaaaactgc ttttttattt gaaat
//
LOCUS       DAYYGY010000061        17800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000061 DAYYGY010000000
VERSION     DAYYGY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.062"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            243..1208
                     /locus_tag="ACHA52_002010"
     CDS             243..1208
                     /locus_tag="ACHA52_002010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8940695.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1307..2479)
                     /locus_tag="ACHA52_002011"
     CDS             complement(1307..2479)
                     /locus_tag="ACHA52_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940696.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2466..3611)
                     /locus_tag="ACHA52_002012"
     CDS             complement(2466..3611)
                     /locus_tag="ACHA52_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940697.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3625..4557)
                     /locus_tag="ACHA52_002013"
     CDS             complement(3625..4557)
                     /locus_tag="ACHA52_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940698.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4674..5753
                     /locus_tag="ACHA52_002014"
     CDS             4674..5753
                     /locus_tag="ACHA52_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8940699.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5750..6379
                     /locus_tag="ACHA52_002015"
     CDS             5750..6379
                     /locus_tag="ACHA52_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8940700.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6489..6716)
                     /locus_tag="ACHA52_002016"
     CDS             complement(6489..6716)
                     /locus_tag="ACHA52_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8940701.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6819..9407)
                     /locus_tag="ACHA52_002017"
     CDS             complement(6819..9407)
                     /locus_tag="ACHA52_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8940702.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9427..10086)
                     /locus_tag="ACHA52_002018"
     CDS             complement(9427..10086)
                     /locus_tag="ACHA52_002018"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8940703.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10135..10638)
                     /gene="trmL"
                     /locus_tag="ACHA52_002019"
     CDS             complement(10135..10638)
                     /gene="trmL"
                     /locus_tag="ACHA52_002019"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8940704.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10859..11725)
                     /locus_tag="ACHA52_002020"
     CDS             complement(10859..11725)
                     /locus_tag="ACHA52_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8940705.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11834..12475
                     /locus_tag="ACHA52_002021"
     CDS             11834..12475
                     /locus_tag="ACHA52_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8940706.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12537..13898)
                     /gene="mgtE"
                     /locus_tag="ACHA52_002022"
     CDS             complement(12537..13898)
                     /gene="mgtE"
                     /locus_tag="ACHA52_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8940707.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13916..14815)
                     /locus_tag="ACHA52_002023"
     CDS             complement(13916..14815)
                     /locus_tag="ACHA52_002023"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8940708.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14817..15614)
                     /locus_tag="ACHA52_002024"
     CDS             complement(14817..15614)
                     /locus_tag="ACHA52_002024"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8940709.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15592..16296)
                     /locus_tag="ACHA52_002025"
     CDS             complement(15592..16296)
                     /locus_tag="ACHA52_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8940710.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16427..17002
                     /locus_tag="ACHA52_002026"
     CDS             16427..17002
                     /locus_tag="ACHA52_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8940711.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17120..17797
                     /locus_tag="ACHA52_002027"
     CDS             17120..17797
                     /locus_tag="ACHA52_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8940712.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5264 a         3817 c         3164 g         5555 t
ORIGIN      
        1 aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt gtttttcggt
       61 cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt aattttgtaa
      121 cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc ttgctaatta
      181 ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag caggaggaaa
      241 tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga aatggctata
      301 aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat acacttcatt
      361 atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa gaaacattga
      421 ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg atcatcgtag
      481 gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa gtagaattcc
      541 cagttgctta tattccagca ggttctggaa atgattttgc tcgaggggca ggccttccaa
      601 aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa aaagtccaca
      661 taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat aatttcggta
      721 taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa aagcgattga
      781 acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct ttattcactc
      841 aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt tcaaacgcat
      901 ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt gttccaactg
      961 ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat ttctttaaaa
     1021 tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa tatttccatc
     1081 actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt caagaagatg
     1141 gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa cagttgtttt
     1201 ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta ttcggaaaaa
     1261 aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa ttctttgccc
     1321 aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc attgaaaaca
     1381 ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca aatgtttgat
     1441 tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat gttggtgtga
     1501 atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac accccaccga
     1561 taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct gagtttttta
     1621 ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag aaagtattta
     1681 aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga accattagaa
     1741 gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt actttgcgat
     1801 tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct ttgcgattaa
     1861 acgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca taagacaaga
     1921 aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc gcacttagct
     1981 caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca tcgattgttt
     2041 tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa
     2101 ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcgccatc tttcctactt
     2161 cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga ttaaaataaa
     2221 gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt tccttgtcat
     2281 tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc aaggcgattt
     2341 tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag aatgtggcaa
     2401 caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc aatgtccaaa
     2461 tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc taacgcaaaa
     2521 aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa atcagtgtag
     2581 aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg accaacaatc
     2641 ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc agcaaaaaag
     2701 ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt catttttggt
     2761 atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag agcggtaaca
     2821 atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg aaagacagct
     2881 aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt agcgattgaa
     2941 actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga ttgatttgct
     3001 tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc cattccaatc
     3061 agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac ttcttgaaaa
     3121 gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc cacttctgcc
     3181 actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt tttcatcaac
     3241 gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc aatgagataa
     3301 aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga tagttcctgt
     3361 acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt aaaattttcc
     3421 gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc aagattgcca
     3481 ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat tggaaaggcc
     3541 aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata tttataaaaa
     3601 tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa
     3661 gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct tttcttcttg
     3721 taagattgta agaatcggca acaatactgt atttagttca gtagttgtta cttggagctg
     3781 acctttttta taagcgattc catcagaagc tattgttcgc tgaatcgctg acaacacatt
     3841 ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag
     3901 agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca
     3961 aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt
     4021 cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg
     4081 ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg
     4141 tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa
     4201 ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca
     4261 aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac
     4321 gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt
     4381 aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc
     4441 actgccgttt ggacctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg
     4501 attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat
     4561 gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact
     4621 agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata
     4681 aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa
     4741 gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg
     4801 tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat
     4861 gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg
     4921 aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta
     4981 atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac
     5041 gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc
     5101 attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa
     5161 cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca
     5221 atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc
     5281 aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg
     5341 atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct
     5401 tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag
     5461 gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca
     5521 gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt
     5581 atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg
     5641 gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag
     5701 aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt
     5761 agtcattatt gatgatgatc catttgtgac gaaatcgtta caaaccattt tagaaagcac
     5821 gaaagagatc cgtgtgttag gcattggtca ttgtgccaag gatgcgctag tcttatacga
     5881 aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa cgggcatcga
     5941 tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt tgacgacatt
     6001 caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt atttaatcaa
     6061 acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac aggccgtatt
     6121 tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga cgattgatag
     6181 tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag tagctgccgg
     6241 gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg ttcgcaatta
     6301 catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg ctatctattt
     6361 ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat gctccttcgc
     6421 atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt tcctcacgat
     6481 tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg cgtgttaggc
     6541 ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg atcgtctcgt
     6601 tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa ctttttaaag
     6661 tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt agccataaag
     6721 taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa agaaaacgct
     6781 aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt tgccggtata
     6841 aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg gttatcgatc
     6901 acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc tttcttttct
     6961 tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg gtttttcgtt
     7021 tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg tggctctttt
     7081 gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata agctgctagt
     7141 ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt caacgtagtc
     7201 atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc ccctaacaaa
     7261 atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg aagcatccgc
     7321 tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg tgatttatgg
     7381 attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata agtgacctca
     7441 tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt tgccaagatg
     7501 atacccgtga tttctcccat atctccattg aatacattca attcgggagt attgactaat
     7561 tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc cttttttcca
     7621 ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat ccctgcaggt
     7681 ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt atttttcgct
     7741 ttttccacaa ttttagagat caagggttca atttgataag catcacttcg gaaataggaa
     7801 cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat ctcatgggca
     7861 agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc cattttggga
     7921 atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga gggaagctga
     7981 tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa taatgtgtta
     8041 gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc ttctaattct
     8101 ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg atggatcgta
     8161 ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt tggtgctgca
     8221 agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag tccgttcaac
     8281 tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc tggtcctcct
     8341 gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg cgaaggtcca
     8401 tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc tttttcagga
     8461 taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat cccccattca
     8521 gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt tccttcttca
     8581 acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg gcgacttgtt
     8641 tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt atctcctgtt
     8701 tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct cggtgaatct
     8761 gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat tccttctatg
     8821 tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt cttataggcc
     8881 tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc catgatcact
     8941 tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt tggcttcgtc
     9001 aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat agcatcttct
     9061 cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa tttttcgctg
     9121 gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata tctttcgagt
     9181 ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg ataagtctca
     9241 ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg gtaatcagct
     9301 gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt ttgaaaaaaa
     9361 atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg aagccgcctc
     9421 cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg tttggttcca
     9481 tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa tcgtcaaact
     9541 actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg tcatccattg
     9601 aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat ctttatgtcg
     9661 ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa tcgtttctcc
     9721 gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca gattatccgg
     9781 atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc ctagttctct
     9841 tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga taccttctgc
     9901 tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa catattttac
     9961 ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag aatcgccatt
    10021 gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg taaaatatag
    10081 ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa ctgtctaatc
    10141 cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt gctggcgaag
    10201 tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga catgctcatc
    10261 attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt ctggcaaacc
    10321 ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat tttcgtcact
    10381 atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta aaaatgttaa
    10441 aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat ccaaccctgc
    10501 ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat gaagcggagt
    10561 attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta atggttcaaa
    10621 taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt ctattttctg
    10681 acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac tgcgagtcgt
    10741 tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat aaaaaccaat
    10801 gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc tctactcatt
    10861 aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg tcaattgtaa
    10921 tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc tgccattcta
    10981 aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat tcataggtta
    11041 cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca cgttcattgg
    11101 aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc tttaaataat
    11161 tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt tggaattctt
    11221 ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca gcaaacggca
    11281 aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta gctgcgtaga
    11341 ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat tttgaaagtt
    11401 cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct tcaggtaaat
    11461 gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt ctacgccttg
    11521 gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa taaagtgttc
    11581 cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta tcagactttt
    11641 ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc aaaaactggc
    11701 gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc ttaccgttag
    11761 atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag tgaaagataa
    11821 aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta ttcctctagc
    11881 aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg gtggcacaac
    11941 cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag atatccccgt
    12001 aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg aattgaaaat
    12061 catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag tatttggttg
    12121 tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg aaacagcaga
    12181 agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc ggcaattcga
    12241 agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg gcggtcctgc
    12301 tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct atacagatgg
    12361 ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac atgtagctaa
    12421 agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt tataatctta
    12481 taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt gtttttttac
    12541 ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact cgtcaaatcg
    12601 ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag acgatccatt
    12661 agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag catagccatc
    12721 cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag aaaaatcgtg
    12781 atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac aagtcgaaca
    12841 aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc tagcgactgg
    12901 gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa aatactcgct
    12961 tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc taaaaacaac
    13021 agagtgatca gccaaggcaa acgtcgagac gctgccttta gtggattttc attaatttct
    13081 tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc aataacgtcg
    13141 atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt aacaggaaga
    13201 gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc gcctacatga
    13261 actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc attcacgatt
    13321 aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac atagatgtaa
    13381 tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga gcgaacagtt
    13441 tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt ttcatcctcg
    13501 taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact taaatattta
    13561 gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt atacatctca
    13621 gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt catatgttcg
    13681 ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag ataggaatag
    13741 atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc atagatatgc
    13801 atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc ttgagtcaac
    13861 tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc tcgccttatt
    13921 cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg aactccagca
    13981 actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct tgtcttttta
    14041 ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag ccaattgcgg
    14101 aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca actaacgcga
    14161 tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct tcatcagaga
    14221 ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata tgtccatgtg
    14281 ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca aacttctttt
    14341 cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa agcatcaagc
    14401 cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg taaccttgtt
    14461 ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat tgggcaggaa
    14521 tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca tacacgatat
    14581 cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga atcacaatgg
    14641 ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca ttaactttga
    14701 tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct ttttctttta
    14761 aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac tgcatatcaa
    14821 tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg ataagaagca
    14881 aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc ttttgcgaca
    14941 ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat ttctagccat
    15001 tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt attcagtgaa
    15061 gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc gccgacactt
    15121 ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc tctaaaactt
    15181 tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc ccgtttaatc
    15241 gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata actgatccga
    15301 acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct gtctactagt
    15361 tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt atgcacgccc
    15421 aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa taatgtgccg
    15481 tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat ccctgcttgt
    15541 tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt attatgaacg
    15601 atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa gcgttcgata
    15661 gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt cttggatttc
    15721 ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg cacgctgaag
    15781 tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg aatgctcaat
    15841 cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa ttttcttctc
    15901 accgctgatc agctgtacag gatactcgat caccagatga taagaacggt agccacttgc
    15961 ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc ttttacggat
    16021 gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta acccggcaat
    16081 atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg attttgttac
    16141 aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat gtcgattttg
    16201 ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata cagtttgttc
    16261 atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta cttcattaac
    16321 attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt tatgcattga
    16381 ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga cacaaatgct
    16441 tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca tcgctcacta
    16501 tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata ccgttgatga
    16561 acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc aagctgaata
    16621 tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct ttataacaga
    16681 agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag tttttcaaaa
    16741 actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc ttacaacaaa
    16801 acgagctgaa ttccctattg aagaaggact tttagctatt gatgaaagct ggggagacaa
    16861 tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa aaattgcttt
    16921 tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca agatccaacg
    16981 tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga gttcattttt
    17041 ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt gtatagaatc
    17101 tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt taggggaata
    17161 ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg ggacaccatc
    17221 aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa ttggggatgt
    17281 catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact tattctcaat
    17341 gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga aaaaaggccg
    17401 ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat attctcaaga
    17461 acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta aagaagatcg
    17521 tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc gggaaatggg
    17581 catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg attttggtgt
    17641 tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt gtaaaacaac
    17701 agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt ctcagttcca
    17761 ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAYYGY010000062        17712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000062 DAYYGY010000000
VERSION     DAYYGY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.063"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..908
                     /locus_tag="ACHA52_002028"
     CDS             78..908
                     /locus_tag="ACHA52_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8940713.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1152..1401)
                     /locus_tag="ACHA52_002029"
                     /pseudo
     CDS             complement(1152..1401)
                     /locus_tag="ACHA52_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1592..1786)
                     /locus_tag="ACHA52_002030"
     CDS             complement(1592..1786)
                     /locus_tag="ACHA52_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940714.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2291..2476)
                     /locus_tag="ACHA52_002031"
     CDS             complement(2291..2476)
                     /locus_tag="ACHA52_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940715.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3326..3667)
                     /locus_tag="ACHA52_002032"
     CDS             complement(3326..3667)
                     /locus_tag="ACHA52_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8940716.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3667..3897)
                     /locus_tag="ACHA52_002033"
     CDS             complement(3667..3897)
                     /locus_tag="ACHA52_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8940717.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4184..4522
                     /locus_tag="ACHA52_002034"
     CDS             4184..4522
                     /locus_tag="ACHA52_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940718.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4727..5289)
                     /locus_tag="ACHA52_002035"
                     /pseudo
     CDS             complement(4727..5289)
                     /locus_tag="ACHA52_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6021..6602)
                     /locus_tag="ACHA52_002036"
     CDS             complement(6021..6602)
                     /locus_tag="ACHA52_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8940719.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6785..7411)
                     /locus_tag="ACHA52_002037"
     CDS             complement(6785..7411)
                     /locus_tag="ACHA52_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8940720.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7433..8764)
                     /locus_tag="ACHA52_002038"
     CDS             complement(7433..8764)
                     /locus_tag="ACHA52_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8940721.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8919..9320)
                     /locus_tag="ACHA52_002039"
     CDS             complement(8919..9320)
                     /locus_tag="ACHA52_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8940722.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9406..9612)
                     /locus_tag="ACHA52_002040"
     CDS             complement(9406..9612)
                     /locus_tag="ACHA52_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940723.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9707..11209)
                     /locus_tag="ACHA52_002041"
     CDS             complement(9707..11209)
                     /locus_tag="ACHA52_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8940724.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11227..11682)
                     /gene="argR"
                     /locus_tag="ACHA52_002042"
     CDS             complement(11227..11682)
                     /gene="argR"
                     /locus_tag="ACHA52_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8940725.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11780..12397)
                     /locus_tag="ACHA52_002043"
     CDS             complement(11780..12397)
                     /locus_tag="ACHA52_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8940726.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12618..13991
                     /locus_tag="ACHA52_002044"
     CDS             12618..13991
                     /locus_tag="ACHA52_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8940727.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14066..15178
                     /locus_tag="ACHA52_002045"
     CDS             14066..15178
                     /locus_tag="ACHA52_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8940728.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15250..16887)
                     /locus_tag="ACHA52_002046"
     CDS             complement(15250..16887)
                     /locus_tag="ACHA52_002046"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8940729.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17055..17663)
                     /locus_tag="ACHA52_002047"
     CDS             complement(17055..17663)
                     /locus_tag="ACHA52_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8940730.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4996 a         3624 c         3109 g         5983 t
ORIGIN      
        1 gcgagcgctt tctttgaatt ttttaatctt tcgtagcaga atgagaatgt attaaaaata
       61 gattaggagg aattttgatg tttagacacc agaaagaatt gcagtttgaa gcaaaaccag
      121 atcgtccgaa tccattaatc gcgaaatatc tgcaggagct gatcggcggc caatatggcg
      181 agatgagtgt agctatgcag tacctgttcc aaggctggag ctgccgagga gaagaaaaat
      241 ataaagatat gctgatggac attgcaacag aagaaatcgg ccacgtggaa atgttggcga
      301 caatggttgc tcgtttatta gagggtgcgc ctgttgaaga tcaagaagca gcgatgcaaa
      361 aagatccaac aatcggtgcc attatctctg gaatgaatcc acagcacgcg atcgtagatg
      421 gcttaggacc tcgtcctgct gacagcgtgg gcaatccatg gagcggtgcc tatatcattg
      481 cgagtggcaa cttgttagca gactttagag ctaatttaaa tgccgaatca caaggaagac
      541 ttcaagtagc acgtatttac gaaatgatcg atgatcgtgg cgtaaaagat atgctgtctg
      601 ttctccttgc aagagatagc tatcatcaaa atctatggga atgtgcagtg aaagaattag
      661 aagaaaaaga aggttcaatc ttagtcccaa gtactttccc gcgtgacaaa gaacgaaaag
      721 acattgctta tgacttctat aacttctcag agggagacca aagtagtgaa ggttcttggg
      781 ctagtggcaa agcactggat ggcgaaggcg aatatcaatg gttgaaagaa cctaagatcg
      841 aaggcgctgc accaaaatta gaaccagtaa caccaaaaat gtatgggaca cctccattca
      901 agaaataaat atctacccgt agaaacaccc gttttttgtt ccacacgctt tgtgagcaaa
      961 actgactaaa gtcattaatc ataaccaccc ttcaaaaggg tggttttctc tagagctata
     1021 agctcaaatc accggccagc gcctaaagac gctggctttc atattttatg ttcaagccat
     1081 attgcctttg ccagttttgc ccacctctta aaaggtttat gtactactcc ttagccctta
     1141 aaaggatcgt cttactctcg tacactcagc ttatctaacg cactatcatg tttttcttgt
     1201 tcttgaatgt actttttgat ggtcgcttca ttttgtccta cttttgtcac atagtatcct
     1261 tccacccaaa agtgtcgata cccaaattta tattttagat ttgcacaatc tcttgatgat
     1321 ctcctgaata ttggctcgat attgattata aattattttt cttctatact ttggtgtgaa
     1381 tacaatgtgg tatttacaca tctactttgt acgggctaaa ctatttccct ttattgctcc
     1441 acccgcatag caagtggttt tgtatttcaa tcgctcggcg cgctcaatta ggtctaaaga
     1501 caataaatct aaaaacccgc tgcttcactg acagaaacaa cgggttttca gaaaaaaatg
     1561 aaatgaaaaa gatctcttac ggcaatctat tttatttttt tgatttttta taaatggaaa
     1621 atgcttcaag aagtccaaga aataaagccg tataaaaaca agaacctatg aaatctttat
     1681 ttattagata ttcaacagaa atcccgataa cactagccaa caatatattt atcagaagtg
     1741 taaaataagg atgcttttta tagaatagaa ctagtgttgt tttcatagac tctcctttct
     1801 tcatatttcc aattaatcac aaattcacgg cattactaga aaaattaaag gggcgcagaa
     1861 gtggaaaaat actcttcgac tttccctaca aaccaggcca tcttgtgttt aataactata
     1921 ccaaatctaa ggtacctcta aatctaaatt ccatttgtgg cggtgacctc caatttttaa
     1981 ctcttctatc tcccatccag tatacataac cttgatactt cgaccactta tatatcgaac
     2041 acacaaatac ctatccattg caggagtact acctaactta gttgctactt gcatctaaat
     2101 aacagctgaa aagataggac ttactcttag taaagtatct gaactattaa tttttaaact
     2161 atcattttta tcctcaacta catgcacatc tgttgcaact tcactttctg tctcatccag
     2221 ataaatgatt aaggaatcgg gaattgcaat atcttcagct gacgctggaa tagttgagct
     2281 aaattagtca tcatatcttt attataaaat aaccaacagc aaaaaataca atgaaaaaca
     2341 aacttggaac taatgtatca ttaataaatg ttgaaaaatt cagcaactca ttactgtatg
     2401 attttagcag cccaaccaac atatacccta taaaagctaa taagatggtt aggaatcctt
     2461 tagaattttt actcattcat agacacttcc ttaaataact tttattatag aaggtattgt
     2521 aaataataat attgacaaaa agtgactcaa aataaaaaaa accttacatt atactggaat
     2581 gaactgtatt gatagtgtac ctaaaagctc tactgacata agatcgtgta atcggtaaag
     2641 taattttatt aattatacgg gatgtaatca attagagaat aaactaatca taaaaggtac
     2701 ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg acccaattgc
     2761 acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa actagcggta
     2821 gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca aatttctctt
     2881 ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa actatagaaa
     2941 ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga tatagtttaa
     3001 aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg tcagttgttc
     3061 tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag atatgtcgtg
     3121 aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc tcaatcattc
     3181 gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat cgaccacact
     3241 agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta gcatataaaa
     3301 atattataga gtttacttac aatgcttaaa acatatactg tataatctga tcaattttag
     3361 ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta aaatcaagag
     3421 attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt aatgagtaac
     3481 gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac attgttttac
     3541 ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt tcttttccta
     3601 cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa attctttctt
     3661 ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt ctttctcata
     3721 atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta acgtaattgt
     3781 tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct ttccgttatc
     3841 agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa caagcattat
     3901 catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat tcccctttgt
     3961 aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa tagaaataaa
     4021 ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat tgaacctatg
     4081 gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt ttgcagataa
     4141 aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg attataaaca
     4201 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
     4261 aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag aaacattggt
     4321 tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg tagctatttt
     4381 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
     4441 tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga attttagatt
     4501 ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa aagactcgca
     4561 gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga ccctatagat
     4621 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
     4681 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
     4741 ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata tctgttaccc
     4801 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
     4861 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
     4921 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
     4981 cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat gaaaggtctt
     5041 tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt taaatccggt
     5101 aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc ttaacgtcac
     5161 aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct tcggttgttt
     5221 cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa aaaaataacg
     5281 accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac aagaagttcg
     5341 caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag tgacaaactc
     5401 tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca
     5461 tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca ggcttacgac
     5521 ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     5581 tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga ctttttcaca
     5641 acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
     5701 aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg gcagcttttt
     5761 cacaacctct ccactaaacg gtgttcaatc agctaacctt cggtattagc tcaaaaaacg
     5821 gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca aagcgctgat
     5881 ttcacaacct ctaatacacg gtgttccaga agtagcttct tcgaaaaata agccgaaatt
     5941 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
     6001 tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag ttaacaatct ttctttgatc
     6061 aattgaaaat tctgcctaag tagtgataga tcctgtgtga ctttcaacaa taagatactt
     6121 ccctggattt cagcaatcaa cgctttggct tgacggtctg cttcttcatc ggacagtttt
     6181 tcttcagaaa gtgccaggat attttgtttc gtatacttga cccattgatt gactaattct
     6241 agtagcaaca gacggtattc ttcatccact gtactcaact caataatcaa attacctaca
     6301 ggacagccat gataagtgat ctgtgtctca tgatcagcga aaatccagtc caacatttct
     6361 tctaatgctt cttctggctg ttttccagca tttaagatgt cttcgaacaa ttctgtctgc
     6421 catacgtcca tatgcgcatg gatcaccgct agacaaactt ctttttttga agaaaagtaa
     6481 taataaaatt gaccttttcc tgtatcagaa gctgctagaa tatctcggat tgacgtttca
     6541 tgatagcctt ttttataaat aagatctttg gtcgcttcca agatcttttc ctttgttttc
     6601 aatgatattc tcctttttca tttctcatag tcctatatta tacagtaaat tcatcatttt
     6661 ttcttcttaa tcgtttaaaa aagactgtgc caaattatct gacacagccc tcactataaa
     6721 actgttctca tatagctaga tgttcattga ttcaagaact gcgaggacaa aagcaattcc
     6781 tatcttatga tagttgctct aatacttcct ctgttgtcat cacttgatct gcaataaagc
     6841 caaagttcgt caacgctgca tccattgctt cttggccagg agcaccaatt gcatctgtcg
     6901 ctaccacaac attgaatcca ctctctacca attcacgcat atgggaatcg ctacacaagt
     6961 ttccgttcat tccaccaaga attactgtgt cgattccatt ttttctcaat tggagcgcca
     7021 aatcattgct ttctggtcca aagattttat gaggagagct gatgatcgta ttttcatcca
     7081 aataaggttt caattcttcg ataaaatcgg ctcctgaacc ttcttccact gattcatatt
     7141 gactttttct agcaaacatc tgctgctcaa gcatcatatg ttctcctgca ccgccaaatt
     7201 gccatttatg gtcatgtggg taaaaataat gaggagaaat aaagagtttg tatccttttt
     7261 cttttgcttg ttttaccatt tcaatcatat tttcgatcgt attgtatttt tctaagatat
     7321 cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc gggtcagtga
     7381 gaactactgc tgtattttta ttattgaaca tggtggattc tccattcgtt atttatttta
     7441 atacatcatt tagagcttca ctcattgtcg gatgagaata gatttgatct ctcaataacg
     7501 tataaggaag ttttccattc atagctagcg aaatcaagtt gatgacttca tgagaatctt
     7561 ccgcatagat acttgcccct agaatcaagt cagtttcagg gtcgactaaa actttgaata
     7621 cacctttagg atcttctaat acttgtgctt tagggacacc gcttgtcata tacttgaata
     7681 atttgtaatt gacgccttgt gctttggctt gtttttctgt taaacctaca ttggataaag
     7741 tcggagtgat aaatacactg taaggcacag ctttccggtt attcgttgtt cgtttattct
     7801 cgcccttcaa ctgatccata atgattcgat aatcatctag agagatataa gtgaactgca
     7861 atccgccttt gacgtctcca atcgcccaaa tgttgtctgc atctgtacga aggtattcat
     7921 ccacagcgat tgctccgcga tctgttagtt taacatctgt attttctaat ccaagtgatg
     7981 ctgtacttgg ttttcttcca gttgcagcca aaacttttga cgcttggatt gttacttctt
     8041 ggccatcttc tgtaaaggtg acggcagctg ttgattcttg gtctgctact tgatctacag
     8101 atacacctag atggaattcg attcccgcat ttgtcatgtc ttctaaaatc atttgcgcga
     8161 tatcttcatc ttcacgtgga agaaattcag aatgggcatc aagaacgaca actttagatc
     8221 cgtattcatt gaacattgat gcaaattcca agccaatata tccagccccg atgatcacta
     8281 atttttccgg taattgttcc tgatccatcg cttgcgtaga atcaatcaag tagcgacttg
     8341 tattcaatcc tttgattggt aaaatgattg gttcagaacc agtattgatg aaaatccgtt
     8401 cgccttcaac aatcactttt tcaccattat tcgtcacttc gatttgatgg ttagaaagaa
     8461 aacgtgctgt accgtccatc actgtcccag ttgcctcatc gctgatcata tgataatttt
     8521 tggcgcgaag catgcccgtc aatttttctt tccgagcaac agcctctgta aagtcgattc
     8581 ctctttctcc attgaaaatc aatgatttag aaggaataca gccgatgttg atacatgttc
     8641 cgccatacat acgtgttgac ttttcaacga ctaaaaccga ttcacctttt gaagctaacg
     8701 ccttcgctaa agtcttaccg ccctttccaa aaccgatcac aatattttga tatttttcca
     8761 tcatttttcc ctcgcttctt ttttacctac cgattggtac aaaaatatga taaccgataa
     8821 ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa aaagcggtcc
     8881 tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt tacttctaca
     8941 tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc ttctgcaatt
     9001 ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac accgatctga
     9061 tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga ctctgctgtc
     9121 attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc ataacctgct
     9181 gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt gattttcata
     9241 tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt gatcattgct
     9301 gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct acttggctat
     9361 aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt cgaataccag
     9421 aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga acaacagcgt
     9481 catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat gattgatttt
     9541 aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa aaataaccat
     9601 attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga tttaaccata
     9661 ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat tctgctgtta
     9721 atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac gccaacttgt
     9781 tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt aagcttaaac
     9841 ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata ccttcttcat
     9901 tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt tcttctaata
     9961 tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt tcttgtactt
    10021 ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt ctttttaatt
    10081 ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga aaatagtttt
    10141 ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc ccaagacggt
    10201 taagttctaa tgctttattg attacatttc gatagccgga aatataacga acttggatgc
    10261 ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc gtcacgattc
    10321 ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa ttatttcgat
    10381 tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta tcgaaaatga
    10441 tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc gcttctttat
    10501 tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat tgggaacctg
    10561 ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca gcaccatttc
    10621 cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt ccaatcccct
    10681 ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct ttttctacgc
    10741 ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca atatcttctt
    10801 tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag ggaagatggc
    10861 tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg gacataaaca
    10921 tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct ttcagacttt
    10981 ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca atcccaatcg
    11041 tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt gctagtcccc
    11101 agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca actggtaatg
    11161 cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata taaaagtcct
    11221 cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt cttcttctga
    11281 gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt cagggtaagc
    11341 cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca atacgataac
    11401 catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac gcaaacgttc
    11461 ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt tgttttcatt
    11521 ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt gagtcacatc
    11581 ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag gttgttctgt
    11641 aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca cctctcaatg
    11701 ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt acttttattt
    11761 tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc gtcttcattt
    11821 tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct ttagaagctc
    11881 tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct ggttcttcgg
    11941 catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt ttgaatacta
    12001 cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct tgtgctatcg
    12061 cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt tcttttttca
    12121 tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt ggatagacag
    12181 tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt attgcatagc
    12241 tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg tcgccgatcc
    12301 ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt tttttcatac
    12361 tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg cgaaattcca
    12421 tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt tgacactatt
    12481 tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac gtttcggcgt
    12541 gtcgagagag aggcggtttc tatttttata atagaaatca ctctcttttt ttacgaaatt
    12601 ttttagaggt gatgtaaatg acaaaacaaa aacagctgct tttagtacct gcagtaattg
    12661 caacagggat gatgtctttt gcgggcgtat tgatcgagac ggccatgaat gtaactttcc
    12721 caactcttat cagacagttt gatttaacga ctgcacaagt ccaatgggta acaacgattt
    12781 atcttttgat gatttcgatc attgttccgc tgtctactta tttgaatcgc aattttagtc
    12841 tcaaaaaact ctttttagct tcaaatctgc ttttcttggc aggtataatg atcgatttct
    12901 tctctcctac cttcatggtc ctattgtttg gtcgattgct ccaaggtgca gcgacaggta
    12961 tcgctcttcc gttgatgttc catattatct tgacatttgc ccccctcaat cgacgaggcg
    13021 cgatgatggg tgtcggtact ttgacaacgg ccattgctcc agcaattggc cccacttatg
    13081 gaggcattat gacttcacat tttacatgga atcatatctt cctgtttctt gtgcccgttt
    13141 tgtttctttc tttgattatc ggctggtttg ctattcccga gatggcagta caaaaaagtg
    13201 gttatttgga ttggataagc gttttaggaa tttgtctttt gtttagtgga agtctaacat
    13261 ttttgagtac gttgggaaca gtttccagct ggattgcttt aatcatcggc ggtattggct
    13321 ggttcctttt ttactatcga agtaaaaaag tggagcatcc actgatccga ctgtctattc
    13381 tttcaaataa aacattccgc ttgtttttga ttggcttctt agtttatcag tttttattac
    13441 ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt agtccttttg
    13501 tcgctggatt agcgatgctt cccggcgcag cggttggtgc tttgctttct cctttttccg
    13561 ggaaaatgct ggatcaatat ggtccgaaga agccaatttt tgctgggctg attctttctt
    13621 tgattggttg gctagcatta ttcctactca taggacacgc ttcagtgggt gtattgattg
    13681 cttgtcatgt gttttatatg atcggtatcg gattatctta cagcaatatg atgaccaccg
    13741 gaatgaatgc actgaatgaa gaactacaag gagacgggaa cgcaatgttc aataccttgc
    13801 agcagttttc tggcgctgtg gctacttcat tagtagcggt cattatcaat tacgtccagc
    13861 atcatacttc ccataactat gaagtgtcta ctacattagg atcaaaagca gcattaggcg
    13921 tcttgttgct gttgcttctc gtaagctttg ctcgattcgc ctattatctg ttctttgcta
    13981 aaaaagcgta gattccatat acggcaggtg ttgcatctgc cgtttttttg tgtgaaaatg
    14041 gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg gcgggatcat
    14101 tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt tatttgatga
    14161 agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt tgtcgaaacg
    14221 cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc ctaaacttat
    14281 aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt tgcttcgacc
    14341 tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag aagcgccaga
    14401 aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc cgttattgaa
    14461 accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag catatttaga
    14521 acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa aagtcacttt
    14581 gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca cagctgatcg
    14641 tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag gctatgctgc
    14701 agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata agatgagtca
    14761 agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca tggatggaaa
    14821 gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc ctactagcga
    14881 agcttataag ggattgacta agagtacaga acagttcttg actgatccag atcagataaa
    14941 aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt tttccccatt
    15001 tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag gctcttctgg
    15061 cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata ctggtcattg
    15121 gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc catcatagaa
    15181 aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact tcttgaaaaa
    15241 gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg cttcatctgg
    15301 caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa gtcgttgttt
    15361 caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta taggtaaata
    15421 gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca cgattctttc
    15481 aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc cagtattcag
    15541 gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat agtcttccct
    15601 aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat ctagctgtct
    15661 ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac caaaagacgc
    15721 tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt tcatgatcca
    15781 aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg cataacgtga
    15841 tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt tttctccttt
    15901 acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg ccggatcatc
    15961 agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag caaaaccatt
    16021 ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac cttccgtatg
    16081 ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag ccatcgtata
    16141 ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa agccattgcg
    16201 atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa agtaacgtaa
    16261 tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat ttggaaatgt
    16321 ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa aggaaacaga
    16381 cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca aataagaata
    16441 caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa attcttcaga
    16501 taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat acacagcttc
    16561 tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga tccgccgagc
    16621 acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa catcagctgg
    16681 taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc ctggactccc
    16741 ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat taccgtaaaa
    16801 tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg tgatttcgtt
    16861 taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca aaaaaacgtt
    16921 ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg aagcaaacga
    16981 gttatttttc acacaaaaaa accgagaact tgaataaaca agtttctcgg tttcatcatt
    17041 ttccttttat cggcttagta ccagccgttt gctaaccaga atgttttagc agcatcccat
    17101 gaaccataac gagatgttac atattgttct gctacacgtt cttgattggc agctgaatag
    17161 tcgccgttta agtatgaagc atctaattga taacggccga tgtaacgtcc gttagtagct
    17221 gtgtatgaac cgcctgattc tttttgtgcg atccattctt ttgcagagct tgaagttggc
    17281 gctgttgcag cagctggggc agcaggagct acttcaactg tttcaacagg tgcaacgtat
    17341 gtatgttctt gcgttgctgt ttcagttgta gatgctgtat tttctgttgt tttgttttct
    17401 gttgttgctg aagaatcatt tgaagttggg attgttaatt gttcaccaac ataaatgcgg
    17461 ttgatgtttg cgatcgaatt tttttcagca attgcatcga tcattgagtt gtctcctgca
    17521 aatttttgtg agatcttaga taatgaatca cctgatttta ctgtataaac ttcatcagcg
    17581 tgagcatttg tagttcctac aaacattgca gtagcaccag cagcaaaagt tgtacctaaa
    17641 acaattttct taaatgaatt catattaaac tccttttttt ctccgttttt tattctttcg
    17701 acgctattaa ct
//
LOCUS       DAYYGY010000063        17327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000063 DAYYGY010000000
VERSION     DAYYGY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.064"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..564)
                     /locus_tag="ACHA52_002048"
     CDS             complement(19..564)
                     /locus_tag="ACHA52_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8940731.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(576..1268)
                     /locus_tag="ACHA52_002049"
     CDS             complement(576..1268)
                     /locus_tag="ACHA52_002049"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8940732.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1287..1571)
                     /gene="macP"
                     /locus_tag="ACHA52_002050"
     CDS             complement(1287..1571)
                     /gene="macP"
                     /locus_tag="ACHA52_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8940733.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1584..2147)
                     /locus_tag="ACHA52_002051"
     CDS             complement(1584..2147)
                     /locus_tag="ACHA52_002051"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8940734.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2375..3037
                     /locus_tag="ACHA52_002052"
     CDS             2375..3037
                     /locus_tag="ACHA52_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8940735.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3161..4357
                     /locus_tag="ACHA52_002053"
     CDS             3161..4357
                     /locus_tag="ACHA52_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8940736.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4458..5834)
                     /locus_tag="ACHA52_002054"
     CDS             complement(4458..5834)
                     /locus_tag="ACHA52_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8940737.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5871..6738)
                     /locus_tag="ACHA52_002055"
                     /pseudo
     CDS             complement(5871..6738)
                     /locus_tag="ACHA52_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6827..7852)
                     /locus_tag="ACHA52_002056"
     CDS             complement(6827..7852)
                     /locus_tag="ACHA52_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8940738.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7870..8544)
                     /locus_tag="ACHA52_002057"
     CDS             complement(7870..8544)
                     /locus_tag="ACHA52_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8940739.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8728..9621)
                     /locus_tag="ACHA52_002058"
     CDS             complement(8728..9621)
                     /locus_tag="ACHA52_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8940740.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9695..11392)
                     /locus_tag="ACHA52_002059"
     CDS             complement(9695..11392)
                     /locus_tag="ACHA52_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8940741.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11385..12698)
                     /locus_tag="ACHA52_002060"
     CDS             complement(11385..12698)
                     /locus_tag="ACHA52_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8940742.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12698..16639)
                     /locus_tag="ACHA52_002061"
     CDS             complement(12698..16639)
                     /locus_tag="ACHA52_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8940743.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16748..17206)
                     /locus_tag="ACHA52_002062"
     CDS             complement(16748..17206)
                     /locus_tag="ACHA52_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8940744.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4860 a         3895 c         3012 g         5560 t
ORIGIN      
        1 tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa aatgtctcaa
       61 tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa tatggagttt
      121 gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg tacatacgcc
      181 ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat ctgttccact
      241 aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct catagtcttg
      301 gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca cattatgcgg
      361 aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat caatggcaaa
      421 aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct ttttttcaat
      481 tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat catatgtgta
      541 gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt gacaaattcg
      601 atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa gctttgtgtc
      661 gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat attgaactga
      721 cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc agggaaattc
      781 gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt aacgatcaat
      841 ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact gcgcaaatat
      901 gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta gccaaatcct
      961 gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc tacttgtaag
     1021 ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc gtattgttgg
     1081 atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg tacgacgatc
     1141 acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc ccatgataat
     1201 ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc gataataccg
     1261 attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa aaccacacat
     1321 aacattagga taacaataat aagagatttc atcaaaaaag agttccattt ggtcgtcttt
     1381 tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc tttacgataa
     1441 aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg ctgttttttt
     1501 aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt aataagcggt
     1561 cctttggcca taagccgatc cctctacttt cctttggtca atagttccca gtattgaatc
     1621 gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg cgcctcttca
     1681 agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg attctcgacc
     1741 ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc tggtgaaaga
     1801 tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc ttctaattct
     1861 ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc agctggaatc
     1921 tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag acgatcttgt
     1981 tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct cttcgaagtt
     2041 cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc gctatagatt
     2101 tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc ttctcctgct
     2161 ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa actccttatt
     2221 agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc tttagtccga
     2281 tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg aaaagaattc
     2341 aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt ggccgtggct
     2401 tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt ttgttctagg
     2461 acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc gcaggaaaaa
     2521 gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact atgaaaaaag
     2581 cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa caaaaaatga
     2641 gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa tcgatctccg
     2701 ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag aaccaaaccg
     2761 actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg atttgacaaa
     2821 taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg aaaaactaga
     2881 agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt atcaaaaatt
     2941 cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac agagcttaaa
     3001 gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat aaaagaagac
     3061 tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta gagacattca
     3121 tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata aaccgactga
     3181 aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca acccgttttc
     3241 aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc tccaagagca
     3301 acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac aagctaaaca
     3361 gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg gatcggctgc
     3421 ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag cacaagatat
     3481 tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag ccaatcctga
     3541 cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg tcaaacaatc
     3601 gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca agatttcagt
     3661 gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag aacagcttta
     3721 ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg gagaattgaa
     3781 aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag aagaaactgg
     3841 cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc gtctggataa
     3901 acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac cacaaatccg
     3961 tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta tcgctacagc
     4021 cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc aaaaagatgc
     4081 agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga aaaattcaga
     4141 aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa tcgtggatat
     4201 cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga cattgcgtat
     4261 ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac acatggaaga
     4321 agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct tgttactaaa
     4381 acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta tctttcttct
     4441 aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc atagccaaaa
     4501 taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac agtttgcaga
     4561 cgaagcatca atacaataca agtcaaaatg gagacgattg gcaatagcgg aacgaatggc
     4621 actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag taagcctaga
     4681 ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa tacattcaat
     4741 gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc ttttttcggt
     4801 gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc cttgctgaca
     4861 gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag tagcgttaaa
     4921 attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct cattgcaaaa
     4981 gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa gatcagcgtc
     5041 acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct tggcacatct
     5101 ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc caagaaagca
     5161 aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat gaatggctgc
     5221 cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat gataccaaat
     5281 ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag cgacacgatg
     5341 taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc attttccgga
     5401 ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa aaaaccttgc
     5461 atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc cccgatcaac
     5521 aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata ggcatatggc
     5581 ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga caaaatgaca
     5641 acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg attggccgca
     5701 acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag catgatcaaa
     5761 tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat ctctttctta
     5821 cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga ttaaactgtt
     5881 ttcatatacc ctaaaaggga taccacctaa aatgactaaa aggcaaccaa caataacata
     5941 aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta aagtagagat
     6001 aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt tgactgttag
     6061 aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt tccacacaaa
     6121 ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca ttccgatact
     6181 ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga tggtatatac
     6241 accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat caagcatttt
     6301 attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac
     6361 aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc attcatggaa
     6421 gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca atggtagccc
     6481 tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa cactccaagc
     6541 taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac taacaacgaa
     6601 aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt gttgattggc
     6661 atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca tagggactaa
     6721 tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa acttatcagc
     6781 gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa cgtttttcat
     6841 tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct tcagttgatt
     6901 cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa agtcctaatc
     6961 ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta tcaaagatcc
     7021 gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg ctgactcctt
     7081 gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat ttgatggcat
     7141 tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa acagtatgat
     7201 cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc tgactgcgta
     7261 tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc cgcgagaaac
     7321 tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt gataattcat
     7381 tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga actagcaatt
     7441 tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa tctttttggt
     7501 cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc agctgattga
     7561 tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa ttttgcaaag
     7621 gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc aagtaaccac
     7681 ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca attgtgctca
     7741 aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca ataaaaaaca
     7801 gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc atcttaaccg
     7861 ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat atccttggat
     7921 acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg tattgtcatc
     7981 tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta agatctttcc
     8041 attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat caatgatctc
     8101 tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt ggctcatcat
     8161 ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga tttttgcaat
     8221 cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt tcatagccat
     8281 gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt tcgatacttc
     8341 acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat ctagtaatac
     8401 aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg tcgttccaaa
     8461 ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga tctcttcgtc
     8521 ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg ctactacaat
     8581 atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt tagcaaacgt
     8641 taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca gcttaaattt
     8701 attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt gtttttgtct
     8761 tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata tacctagaag
     8821 gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg aagagacttc
     8881 ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg
     8941 tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg tatttgtttt
     9001 tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac catacacttc
     9061 aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt ttttctcagc
     9121 aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg gaaaagaggt
     9181 gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat caaataaagg
     9241 caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt cgtaatattc
     9301 ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat
     9361 tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca ctgctaattg
     9421 ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct cgatccttct
     9481 ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc
     9541 ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg tttgaggtct
     9601 attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg cagcaacctt
     9661 tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc attttagatt
     9721 tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag aatgccaaga
     9781 gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact ctttctggcc
     9841 ctggattttc tactcctacg atctgaattt cttcattata gcttccataa gagatgataa
     9901 attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc atggtctctt
     9961 tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt ccatctgcga
    10021 caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct gattcataaa
    10081 cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct tcattccaca
    10141 attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt tcaaactttc
    10201 ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca aattcatcat
    10261 ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct agttctgtgg
    10321 aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct aatctaccta
    10381 aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc
    10441 ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt
    10501 ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt
    10561 tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata ggtgctgtga
    10621 tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt tcttgttctc
    10681 cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc atctgtttat
    10741 ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat gtgtctttcc
    10801 atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc atcataacaa
    10861 ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag aaaagataga
    10921 ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat accgcttctc
    10981 cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt
    11041 ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg tcttctcgtt
    11101 ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat
    11161 caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat ccgctattcg
    11221 gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg
    11281 gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc cctattcccg
    11341 cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc atttttttct
    11401 ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc tagtaaagaa
    11461 atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga ttgacctgga
    11521 ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt ctcgttataa
    11581 acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc taattcagcc
    11641 atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac ccaaaattca
    11701 gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc cacacctttt
    11761 aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc
    11821 tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt gtctgctggt
    11881 ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata tttcccccct
    11941 tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt ttcagttgtt
    12001 cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac atttttagga
    12061 tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat tggcaaaata
    12121 gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc tggatcaatc
    12181 gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg tgctttgata
    12241 cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac cgaagtccct
    12301 atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt cgtttcagcg
    12361 atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc atgataataa
    12421 tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata
    12481 tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg gtattcaaat
    12541 tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc cacgacatct
    12601 tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa
    12661 acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc tcctttttag
    12721 attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat gcagcaagca
    12781 ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca ggatcgttcc
    12841 cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct aactcttgct
    12901 gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact tttagtgtac
    12961 ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct cgataactaa
    13021 tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc acttccttga
    13081 tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg tttggatctg
    13141 gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc tcaattgaaa
    13201 attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt tccaaatcga
    13261 ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt tcatagcctt
    13321 ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac ttaccttctt
    13381 cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt gcgacagatt
    13441 ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt aaatcgacat
    13501 acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa cgtttcgatt
    13561 cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa gaatccccca
    13621 ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct aattttctaa
    13681 ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat aaacgatcct
    13741 cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc ttgagttgga
    13801 tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca tcaggttctt
    13861 tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg gtagtttctt
    13921 cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact ttctcaccat
    13981 ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc cctttctctt
    14041 cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt tccagtccgt
    14101 taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta ctgccttcaa
    14161 ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc agtttgacat
    14221 acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa agcttgtgat
    14281 attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact ctcgaaccag
    14341 atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg gctgcatatt
    14401 ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt ccagcgatct
    14461 ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg
    14521 tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt tcggcttccc
    14581 cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc tcgccatttt
    14641 gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg gaagaagatg
    14701 ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc tgaatatagg
    14761 ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt tttacttggt
    14821 acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta taagcttggt
    14881 tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct tgtccatgac
    14941 gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct ggatctagat
    15001 ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta aaaccagttt
    15061 catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata tcctccaagg
    15121 ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt
    15181 ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt tgtctcccgc
    15241 taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc tcttgatact
    15301 tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt tcttgcgaag
    15361 taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga gtcgctttat
    15421 tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct tctggacggt
    15481 taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta ttcccagaaa
    15541 actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct tccatcacag
    15601 tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc ggtgcataac
    15661 tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt ctattttctc
    15721 cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta agcggagttc
    15781 ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga ttgatatcat
    15841 ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct gtactattat
    15901 ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc agatctttac
    15961 tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg gtcccagcgg
    16021 ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt ggactgattt
    16081 cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt gcatcttcag
    16141 gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt ttgatgtacg
    16201 ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa agctgaacgg
    16261 tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta gtagcatagt
    16321 cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc aagttctctc
    16381 cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc caatttccta
    16441 acacttttgt atagtcattc gttgtaaact tcgggtagcg atgctgttcg ttttcaactt
    16501 ctccgctagg atagatatat tcataggggt cttgcgtagc tgttggccca agacgctcca
    16561 aaataccgtt gaataactgg cgttgaatgc gctggagtgc ttgaccgctg ggtacgattg
    16621 gttcgtccag cttttccatc tcattttctt cgctacgttg aatttcagcg gaatcttctg
    16681 tatcagtttc attttcttgt aattcttggt tatctattct ttcatcaacc accacttgaa
    16741 gaggtagtta cccttcgttc acatcgggga tcttttctgt ttcaaaaatc aatgtgccgg
    16801 tttctgcttc ttttgaataa acttttccga cataccattc ttgaccatcg atcgtctcga
    16861 cttttaggtc ttctccatct tcatcatagg aggatagacc tgttccacga acgtttttta
    16921 cggtaccaat cccggcggct gccgcatcga ttgctaaacg tattctacct tcattggcac
    16981 tttccttttt ttcaaaagcc agatgccatt caagggtttc ttctttttca atatacttcc
    17041 cttcaatttt tgcatagtcc tcatctaaga atacctcatc ctcctttgtt tctgttgact
    17101 cagcagctac atccagggtc gtcatatgat agactgactg gaaatatggt ccaaatagca
    17161 aactgacagt tactaactgc aaaagcttca attttaaccc tttcacatcc ttatactcct
    17221 ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac aatgcccatc taattataat
    17281 ttaatttaat tttaatttta aatcaaatga tatccttttt agttaaa
//
LOCUS       DAYYGY010000064        16647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000064 DAYYGY010000000
VERSION     DAYYGY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.065"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..793
                     /locus_tag="ACHA52_002063"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACHA52_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
     gene            933..1859
                     /gene="cysK"
                     /locus_tag="ACHA52_002064"
     CDS             933..1859
                     /gene="cysK"
                     /locus_tag="ACHA52_002064"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8940745.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLGTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2095..2541
                     /locus_tag="ACHA52_002065"
     CDS             2095..2541
                     /locus_tag="ACHA52_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8940746.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2813..4165
                     /locus_tag="ACHA52_002066"
     CDS             2813..4165
                     /locus_tag="ACHA52_002066"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8940747.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4336..4623
                     /locus_tag="ACHA52_002067"
     CDS             4336..4623
                     /locus_tag="ACHA52_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8940748.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4726..5292
                     /locus_tag="ACHA52_002068"
     CDS             4726..5292
                     /locus_tag="ACHA52_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8940749.1"
                     /translation="MTSFVNPFFSWFQIGFSYKIFLSLLNRKQKNNIMKEKEGVEMDK
                     RQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGKIPDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5298..6482)
                     /locus_tag="ACHA52_002069"
     CDS             complement(5298..6482)
                     /locus_tag="ACHA52_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8940750.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6750..7448
                     /locus_tag="ACHA52_002070"
     CDS             6750..7448
                     /locus_tag="ACHA52_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8940751.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7455..8132
                     /gene="nth"
                     /locus_tag="ACHA52_002071"
     CDS             7455..8132
                     /gene="nth"
                     /locus_tag="ACHA52_002071"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8940752.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8258..8791)
                     /locus_tag="ACHA52_002072"
     CDS             complement(8258..8791)
                     /locus_tag="ACHA52_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940753.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9088..11469)
                     /locus_tag="ACHA52_002073"
     CDS             complement(9088..11469)
                     /locus_tag="ACHA52_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8940754.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11459..12106)
                     /gene="recU"
                     /locus_tag="ACHA52_002074"
     CDS             complement(11459..12106)
                     /gene="recU"
                     /locus_tag="ACHA52_002074"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8940755.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12170..12709
                     /locus_tag="ACHA52_002075"
     CDS             12170..12709
                     /locus_tag="ACHA52_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8940756.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12799..13218
                     /gene="gpsB"
                     /locus_tag="ACHA52_002076"
     CDS             12799..13218
                     /gene="gpsB"
                     /locus_tag="ACHA52_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8940757.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13240..13607
                     /gene="rnpB"
                     /locus_tag="ACHA52_002077"
     ncRNA           13240..13607
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA52_002077"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13816..14982
                     /locus_tag="ACHA52_002078"
     CDS             13816..14982
                     /locus_tag="ACHA52_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8940758.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15028..16524
                     /locus_tag="ACHA52_002079"
     CDS             15028..16524
                     /locus_tag="ACHA52_002079"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8940759.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
BASE COUNT         5627 a         2864 c         3443 g         4713 t
ORIGIN      
        1 gaacgagtct tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag
       61 atgaacaagg atctcaaaca gttgctgatc ctcaagcgtt tatcgatcaa atcggctggt
      121 ctgcgtcaga agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt
      181 tgttggaaag tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg
      241 gtgtgaaagg aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaacgg
      301 acaatgggta acgatgaatg aaccattccg aaaatattct tcttattggg aatccttcca
      361 agaccatgcg aatgttcttc gttcaaccag tttttccacg ggcgattatc attatgctgg
      421 cgtgtggaaa agcaatacaa caagttttta tgatgcaaca gcgtacttga caggtcgtta
      481 tgctaccgat ccaaattatg gggccaaatt gaactcattg attaatactt ataatttaac
      541 ccgttttgat acgccatcaa caggttcggc taccacaagt acaacaacaa cgactactac
      601 gacgacttca acgtcatcat cttcaacaac agatgtaaat acaaataatg gtcagacata
      661 tacagtcgtg tcaggtgata cgctatggga tattgcacaa aaatttggca tgtctgttga
      721 tgatctaatg gcgaaaaatg gcatgtctat gtccagttat tctttattag ttggtcaaga
      781 actaaacgtt tgatttagat aaaaaggcaa agaaactacc atatatggta tatctttgtc
      841 ttttttttgc tagtcaaaga cgaatcactc gccataaatg aacagattgt attatgataa
      901 gtaagaagag caaagacaag gaggaattga agatgacagc tatctataac tcagtcaccg
      961 aattgattgg caaaacgcca atcgtcaaat taaataaaat cgtaccagaa gattcagcag
     1021 atgtttttgt aaagcttgag ttttttaatc caggaggaag tgtaaaagat cgtattgctt
     1081 taagtatgat agaaaaagca gaacacgatg gtctactgaa accaggtgat actatcattg
     1141 aacctacttc tggaaataca ggaatcggat tgtcgatggt aggagttgca aaaggatata
     1201 aagtaatcat cgtgatgcct gagacgatga gtatcgaaag acgtttattg atgaaagggt
     1261 acggcgcaga gttgattttg actcctggtg cggatggtat ttctggatca atcagagaag
     1321 cagaacgttt ggcaaaagaa aacggctact ttttaccatt acaatttgaa aatgaagcaa
     1381 atcctcttgt gcatgagaag acgactggtc ctgagataca tcaagcattt ggagtaaatg
     1441 gcttggacgc gtttgttgca ggtatcggta caggaggaac gatcactggc gctggccgag
     1501 aattaaaacg tgtgtatccg aaaatcgaat taatcggggt agaaccagca gaatctgcta
     1561 ttttagaagg gaaagaagcg ggacctcaca aaatccaagg aatcggtaca ggttttgttc
     1621 ctaaaacact gggtacttct gtgtatgata aagtcctttc tattagtgga gacgaagcga
     1681 tggaaacagc tagagaagtt gggcgaaaag aagggatttt agtaggcatc tcatctggcg
     1741 ctgcaattgc tgccgcgcta aaagtcgcaa aagaattagg aaaaggcaag aaggtattag
     1801 ctgtggttcc cgacaatggt gaacgttatt tatctacagc cctttaccaa gagatataag
     1861 gcacttggtt tttctccaga taggtttatg taaaaaactt ttaaagatga aaagaacaca
     1921 caaaaattta tgatgagaaa aattatttga atagagacgt aaaatagtga acgcgactgt
     1981 tttacgtctt ttttcattaa aatacaataa aattgaattt tcttttcatg ttaagtggat
     2041 tttaaaatgt aaaaatagta tgataagacg agaataacaa cgtggggtgt ttacatggaa
     2101 aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag agaacattcg aatcacacct
     2161 caacggtacg cggttttaga atatttgatt gaacatcatt cccatccaac ggctgatgag
     2221 atttataaag cacttgaaca tcggtttcca aatatgagtg ttgcgactgt ttacaataac
     2281 cttcgcttat ttacttcgat tggatttgtt caagaaatga agtacggcga tgcttctagc
     2341 cgatttgatt tcagttcaaa gcgtcattat catgtaattt gtgaaaactg cggtaagatt
     2401 gttgattttc attacccagg gcttgaagat atagaaatgg ctgcgagcag actgactgat
     2461 tttgagatta atgaacatcg gttggaattg tatggactct gtccggattg taaagagtca
     2521 gcaaaaaagg cgcaaatgta aaaacagttt ctataagtgt tatacctaaa aaaagctagc
     2581 tgtgtaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatatca
     2641 aagtgaatat tatcacaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2701 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2761 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     2821 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     2881 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     2941 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3001 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3061 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3121 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3181 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3241 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3301 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3361 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3421 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3481 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3541 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3601 caaagtgaat gaatacatgc agacaagtga tccagacatt tttgcagctg gagactctgc
     3661 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3721 tcgtcaaggt ttacttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3781 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     3841 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     3901 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     3961 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4021 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4081 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagaagcgct
     4141 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4201 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4261 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4321 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4381 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4441 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4501 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4561 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4621 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4681 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4741 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4801 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     4861 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     4921 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     4981 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5041 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5101 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5161 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5221 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5281 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5341 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5401 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5461 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5521 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5581 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5641 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5701 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5761 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     5821 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     5881 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     5941 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6001 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6061 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6121 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6181 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6241 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6301 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6361 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6421 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6481 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6541 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6601 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6661 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6721 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6781 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     6841 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     6901 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     6961 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7021 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7081 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7141 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7201 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7261 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7321 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7381 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7441 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7501 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7561 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7621 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7681 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7741 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7801 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     7861 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     7921 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     7981 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8041 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8101 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8161 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8221 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8281 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8341 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8401 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8461 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8521 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8581 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8641 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8701 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8761 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     8821 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     8881 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     8941 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9001 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9061 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9121 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9181 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9241 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9301 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9361 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9421 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9481 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9541 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9601 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9661 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9721 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9781 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
     9841 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
     9901 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
     9961 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10021 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10081 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10141 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10201 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10261 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10321 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10381 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10441 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10501 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10561 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10621 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10681 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10741 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10801 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    10861 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    10921 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    10981 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11041 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11101 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11161 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11221 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11281 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11341 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11401 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11461 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11521 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11581 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11641 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11701 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11761 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    11821 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    11881 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    11941 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12001 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12061 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12121 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12181 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12241 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12301 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12361 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12421 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12481 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12541 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12601 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12661 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12721 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12781 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    12841 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    12901 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    12961 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13021 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13081 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13141 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13201 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13261 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13321 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13381 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13441 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13501 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13561 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13621 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13681 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13741 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13801 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    13861 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    13921 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    13981 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14041 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14101 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14161 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14221 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14281 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14341 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14401 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14461 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14521 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14581 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14641 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14701 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14761 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    14821 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    14881 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    14941 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15001 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15061 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15121 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15181 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15241 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15301 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15361 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15421 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15481 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15541 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15601 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15661 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15721 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15781 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    15841 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    15901 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    15961 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16021 ttttatcgtt tattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16081 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16141 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16201 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16261 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16321 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16381 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16441 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16501 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16561 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16621 tgatcgctga ttatagtatg gaccaat
//
LOCUS       DAYYGY010000065        16281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000065 DAYYGY010000000
VERSION     DAYYGY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.066"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1023)
                     /gene="murB"
                     /locus_tag="ACHA52_002080"
     CDS             complement(88..1023)
                     /gene="murB"
                     /locus_tag="ACHA52_002080"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8940760.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1096..2016)
                     /locus_tag="ACHA52_002081"
     CDS             complement(1096..2016)
                     /locus_tag="ACHA52_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8940761.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2129..2881
                     /locus_tag="ACHA52_002082"
     CDS             2129..2881
                     /locus_tag="ACHA52_002082"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8940762.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3745..4278
                     /locus_tag="ACHA52_002083"
     CDS             3745..4278
                     /locus_tag="ACHA52_002083"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8940763.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4768..5292)
                     /locus_tag="ACHA52_002084"
     CDS             complement(4768..5292)
                     /locus_tag="ACHA52_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8940764.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5285..5758)
                     /gene="tsaE"
                     /locus_tag="ACHA52_002085"
     CDS             complement(5285..5758)
                     /gene="tsaE"
                     /locus_tag="ACHA52_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8940765.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5881..6861)
                     /gene="pta"
                     /locus_tag="ACHA52_002086"
     CDS             complement(5881..6861)
                     /gene="pta"
                     /locus_tag="ACHA52_002086"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8940766.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7014..7694)
                     /locus_tag="ACHA52_002087"
     CDS             complement(7014..7694)
                     /locus_tag="ACHA52_002087"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8940767.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7820..8689
                     /locus_tag="ACHA52_002088"
     CDS             7820..8689
                     /locus_tag="ACHA52_002088"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8940768.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8715..9230
                     /locus_tag="ACHA52_002089"
     CDS             8715..9230
                     /locus_tag="ACHA52_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8940769.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9277..9840)
                     /locus_tag="ACHA52_002090"
     CDS             complement(9277..9840)
                     /locus_tag="ACHA52_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8940770.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9895..10074)
                     /locus_tag="ACHA52_002091"
     CDS             complement(9895..10074)
                     /locus_tag="ACHA52_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940771.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10151..10660)
                     /locus_tag="ACHA52_002092"
     CDS             complement(10151..10660)
                     /locus_tag="ACHA52_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940772.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10756..11136)
                     /locus_tag="ACHA52_002093"
     CDS             complement(10756..11136)
                     /locus_tag="ACHA52_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8940773.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11466..12215
                     /gene="pnuC"
                     /locus_tag="ACHA52_002094"
     CDS             11466..12215
                     /gene="pnuC"
                     /locus_tag="ACHA52_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8940774.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12399..13676)
                     /locus_tag="ACHA52_002095"
     CDS             complement(12399..13676)
                     /locus_tag="ACHA52_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940775.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13755..14399)
                     /gene="pcp"
                     /locus_tag="ACHA52_002096"
     CDS             complement(13755..14399)
                     /gene="pcp"
                     /locus_tag="ACHA52_002096"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8940776.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14598..15542)
                     /locus_tag="ACHA52_002097"
     CDS             complement(14598..15542)
                     /locus_tag="ACHA52_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8940777.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15544..16230)
                     /locus_tag="ACHA52_002098"
     CDS             complement(15544..16230)
                     /locus_tag="ACHA52_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8940778.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4689 a         3380 c         2879 g         5333 t
ORIGIN      
        1 gatttgcgcc atttgcgtaa acaaccacaa aaaagagcag gacggccacc gtcctgctct
       61 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
      121 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      181 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      241 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      301 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      361 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      421 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      481 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      541 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      601 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      661 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      721 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      781 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      841 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      901 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      961 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
     1021 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1081 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1141 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1201 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1261 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1321 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1381 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1441 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1501 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1561 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1621 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1681 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1741 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1801 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1861 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1921 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1981 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     2041 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2101 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2161 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2221 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2281 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2341 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2401 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2461 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2521 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2581 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2641 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2701 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2761 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2821 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2881 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2941 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     3001 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3061 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3121 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3181 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3241 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3301 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3361 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3421 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3481 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3541 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3601 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3661 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3721 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3781 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3841 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3901 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3961 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     4021 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4081 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4141 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4201 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4261 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4321 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4381 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4441 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4501 ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa
     4561 tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc
     4621 gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga
     4681 ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga
     4741 cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc
     4801 taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc
     4861 catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg
     4921 aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag
     4981 tcccattccc caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg
     5041 aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag
     5101 cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg
     5161 tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc
     5221 catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac
     5281 ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt
     5341 gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta
     5401 aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa
     5461 cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta
     5521 cccgataaat atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg
     5581 tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca
     5641 acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg
     5701 aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt
     5761 ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa
     5821 agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg
     5881 ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt
     5941 tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc
     6001 ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg
     6061 aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac
     6121 tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag
     6181 ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc
     6241 ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt
     6301 tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca
     6361 atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt
     6421 ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt
     6481 cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc
     6541 aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct
     6601 acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat
     6661 cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt
     6721 ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat
     6781 acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct
     6841 taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca
     6901 ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat
     6961 taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact
     7021 gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt
     7081 ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga
     7141 gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt
     7201 ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct
     7261 gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga
     7321 acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca
     7381 ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc
     7441 tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga
     7501 tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag
     7561 atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt
     7621 agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga
     7681 atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc
     7741 acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca
     7801 gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt
     7861 agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc
     7921 cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga
     7981 tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga
     8041 aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat
     8101 tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa
     8161 tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac
     8221 gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt
     8281 ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt
     8341 tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga
     8401 tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa
     8461 aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac
     8521 gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat
     8581 gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga
     8641 atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga
     8701 aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc
     8761 ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa
     8821 caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc
     8881 agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa
     8941 ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga
     9001 cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca
     9061 tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg
     9121 atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta
     9181 aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac
     9241 ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc
     9301 ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa
     9361 ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt
     9421 cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc
     9481 ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa
     9541 caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat
     9601 gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc
     9661 ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa
     9721 aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag
     9781 aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat
     9841 agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt
     9901 taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc
     9961 agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt
    10021 gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc
    10081 tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa
    10141 tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga
    10201 ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt
    10261 tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt
    10321 tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc
    10381 cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg
    10441 cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt
    10501 cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt
    10561 cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta
    10621 ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt
    10681 ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg
    10741 acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa
    10801 cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga
    10861 ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc
    10921 acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc
    10981 agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct
    11041 gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac
    11101 aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta
    11161 agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc
    11221 tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac
    11281 tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca
    11341 gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc
    11401 atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct
    11461 catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa
    11521 attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca
    11581 tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat
    11641 ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta
    11701 ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga
    11761 tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa
    11821 tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc
    11881 ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca
    11941 ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat
    12001 attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc
    12061 atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc
    12121 ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg
    12181 aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta
    12241 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct
    12301 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    12361 ttcttggagt taaacgcttc tgtcccagcc tctatttttt atagtaattc cttcagatac
    12421 tttcttgcct catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct
    12481 tgtttctgag cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc
    12541 acatttccag attggatatc tttcactact agttgccatt tttcttttga gactgtacgg
    12601 tctttcttat cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca
    12661 tttctgacta tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat
    12721 tgcttccaaa taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat
    12781 tccatctgag cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata
    12841 aatgtttcaa aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa
    12901 tctttacgta aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct
    12961 tctgcaaagt cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg
    13021 tgttcactta ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc
    13081 atttctactt ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc
    13141 tgtttcatcc gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga
    13201 aaatcgatat tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc
    13261 attccctgat ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg
    13321 ttgatgccta gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt
    13381 aattctctac cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga
    13441 ttgatcttga agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa
    13501 cgagagctga ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat
    13561 tttgcataga tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag
    13621 cttttgctga tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct
    13681 gtttctttcc tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag
    13741 tgtttcttgc taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct
    13801 actgctgctt caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc
    13861 gtacccactg gacgatcgat cacttgatcg ggtgagaatg gcacgtggat gaacccacct
    13921 cgaattgccg ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag
    13981 acaaatgttc ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt
    14041 gtcattgctt tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc
    14101 gtatcgaatg gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg
    14161 acacgttcga acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct
    14221 ggttggtgct gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt
    14281 acttccactt tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca
    14341 taggctggat tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc
    14401 tgttgttcct ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt
    14461 gaacagcgta aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat
    14521 tcttataaaa aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc
    14581 acaacccctc tcttctatta tttaaataga atcatataag caatttggaa aactagcata
    14641 aataacgcaa taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc
    14701 gctacaggca cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg
    14761 caagtcaatg caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt
    14821 gcaccgatcc caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta
    14881 aatatcacca tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg
    14941 atcgtaccta ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct
    15001 agagaagcta aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct
    15061 gcatcatcta aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg
    15121 gctgcaaaaa caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac
    15181 aagatcgcaa agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct
    15241 gacatttttt ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta
    15301 gcaattgctg gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat
    15361 cttcctcctg caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga
    15421 ggattggttt tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg
    15481 atataaagga tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc
    15541 atcttattgc ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa
    15601 gtagacactt gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg
    15661 tcccaatgtt acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc
    15721 tcctccgatg aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat
    15781 accttttagt tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc
    15841 caatgccatc ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt
    15901 aaaagccgca aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac
    15961 tttcttgaat cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt
    16021 tcctgttgcc aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt
    16081 acctaaaagt tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa
    16141 agcaaccatt acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa
    16201 gacgcctaaa aggctaagat aattttccat atattttcct ccataaaatg acaatatttt
    16261 tttaattata aagcaaaaca c
//
LOCUS       DAYYGY010000066        16257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000066 DAYYGY010000000
VERSION     DAYYGY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.067"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHA52_002099"
     CDS             226..894
                     /locus_tag="ACHA52_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8940779.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="ACHA52_002100"
     CDS             complement(1094..1324)
                     /locus_tag="ACHA52_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940780.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1321..1479)
                     /locus_tag="ACHA52_002101"
     CDS             complement(1321..1479)
                     /locus_tag="ACHA52_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940781.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKMKKKHA
                     NSLFLNNK"
     gene            complement(1451..2833)
                     /locus_tag="ACHA52_002102"
     CDS             complement(1451..2833)
                     /locus_tag="ACHA52_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8940782.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHA52_002103"
     CDS             complement(2989..4176)
                     /locus_tag="ACHA52_002103"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8940783.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHA52_002104"
     CDS             complement(4183..5214)
                     /locus_tag="ACHA52_002104"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8940784.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACHA52_002105"
     CDS             complement(5260..6684)
                     /locus_tag="ACHA52_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8940785.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACHA52_002106"
     CDS             6859..7764
                     /locus_tag="ACHA52_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8940786.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHA52_002107"
     CDS             7855..8532
                     /locus_tag="ACHA52_002107"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8940787.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHA52_002108"
     CDS             8529..8699
                     /locus_tag="ACHA52_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940788.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHA52_002109"
     CDS             8813..9709
                     /locus_tag="ACHA52_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8940789.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHA52_002110"
     CDS             complement(9764..11134)
                     /locus_tag="ACHA52_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8940790.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHA52_002111"
     CDS             11372..12118
                     /locus_tag="ACHA52_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8940791.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHA52_002112"
     CDS             12115..12954
                     /locus_tag="ACHA52_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8940792.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13904
                     /locus_tag="ACHA52_002113"
                     /pseudo
     CDS             12955..13904
                     /locus_tag="ACHA52_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14045..14797
                     /locus_tag="ACHA52_002114"
     CDS             14045..14797
                     /locus_tag="ACHA52_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940793.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="ACHA52_002115"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="ACHA52_002115"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8940794.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5282 a         3020 c         3254 g         4701 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac
    13501 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
    13561 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
    13621 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
    13681 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
    13741 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
    13801 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
    13861 ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag
    13921 cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac attattcaaa gtttttgtcc agtaattgtt gtaacaatta
    16021 ctggtttttt tgtatttttt acattttttt aagagaattt gataaaaatc aaaaattttt
    16081 ttgctttttt ttatggatta ttgcagtgaa tgttacagaa tctgcgtttt tcattcaaaa
    16141 ataaaaaaac atagaaaaac gattactttg cttatacaac aaggctttta tggttttgta
    16201 tcaaaaaaat atttgtttaa atgttacaaa tattaaataa ttcgttattt tgtcatt
//
LOCUS       DAYYGY010000067        16160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000067 DAYYGY010000000
VERSION     DAYYGY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.068"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            243..890
                     /locus_tag="ACHA52_002116"
     CDS             243..890
                     /locus_tag="ACHA52_002116"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8940795.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            1173..1246
                     /locus_tag="ACHA52_002117"
     tRNA            1173..1246
                     /locus_tag="ACHA52_002117"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1207..1209,aa:Ile,seq:cat)
     gene            1794..2768
                     /gene="bsh"
                     /locus_tag="ACHA52_002118"
     CDS             1794..2768
                     /gene="bsh"
                     /locus_tag="ACHA52_002118"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8940796.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            3093..4988
                     /gene="asnB"
                     /locus_tag="ACHA52_002119"
     CDS             3093..4988
                     /gene="asnB"
                     /locus_tag="ACHA52_002119"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8940797.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(5183..5629)
                     /locus_tag="ACHA52_002120"
     CDS             complement(5183..5629)
                     /locus_tag="ACHA52_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8940798.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5857..6006
                     /gene="rpmG"
                     /locus_tag="ACHA52_002121"
     CDS             5857..6006
                     /gene="rpmG"
                     /locus_tag="ACHA52_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8940799.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            6094..6612
                     /locus_tag="ACHA52_002122"
     CDS             6094..6612
                     /locus_tag="ACHA52_002122"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8940800.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6689..7645)
                     /locus_tag="ACHA52_002123"
     CDS             complement(6689..7645)
                     /locus_tag="ACHA52_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8940801.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7811..8617
                     /locus_tag="ACHA52_002124"
     CDS             7811..8617
                     /locus_tag="ACHA52_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940802.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8614..9603
                     /locus_tag="ACHA52_002125"
     CDS             8614..9603
                     /locus_tag="ACHA52_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8940803.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            9600..10604
                     /locus_tag="ACHA52_002126"
     CDS             9600..10604
                     /locus_tag="ACHA52_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8940804.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10736..11278
                     /locus_tag="ACHA52_002127"
     CDS             10736..11278
                     /locus_tag="ACHA52_002127"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8940805.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            11298..11996
                     /locus_tag="ACHA52_002128"
     CDS             11298..11996
                     /locus_tag="ACHA52_002128"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8940806.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            12017..12229
                     /locus_tag="ACHA52_002129"
     CDS             12017..12229
                     /locus_tag="ACHA52_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8940807.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            12229..13191
                     /locus_tag="ACHA52_002130"
     CDS             12229..13191
                     /locus_tag="ACHA52_002130"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8940808.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            13209..13604
                     /locus_tag="ACHA52_002131"
     CDS             13209..13604
                     /locus_tag="ACHA52_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8940809.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13767..14132
                     /locus_tag="ACHA52_002132"
     CDS             13767..14132
                     /locus_tag="ACHA52_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8940810.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            14129..15940
                     /locus_tag="ACHA52_002133"
     CDS             14129..15940
                     /locus_tag="ACHA52_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8940811.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(16002..>16160)
                     /locus_tag="ACHA52_002134"
     CDS             complement(16002..>16160)
                     /locus_tag="ACHA52_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8940812.1"
                     /translation="FVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKK
                     AMRKRVSR"
BASE COUNT         5070 a         2683 c         3353 g         5054 t
ORIGIN      
        1 ttttgagggg aagtacataa tgcaaatttg tccataactt attgattatc ttgtaaaaat
       61 gcggtaattt aatagttagt gagaagaggt gcttaattat agttaaaaaa gaggaaatag
      121 aagcagcatg taaccaagca aatgttaggt ttattggaga agctttatct ggatatccta
      181 agtataaata cttacacaac tggttacaaa gtaaaagttt ttatcttaat aaagaaagat
      241 gcatgcctaa taaaaattat agggtttata aaagaggaac tattgtatat gttgattttg
      301 gggtgaacgt gggttatgag ttatctggta atcattttgc tattgtttta aataataaag
      361 ataattcgaa aaatggtgtt gtatgtgtag taccaatctc ttcaaaaaag aaaagtaatt
      421 atgtatctgt aggaaaaata ttagaagttg ctagtattaa acaatttata agccaagctg
      481 ataaattaaa agagaaactt aggataatta ttttactttc attaaataga ggcatcattg
      541 atgaaagcga tttgaatagt tttattaaag atattgaagc taaaggtgaa aagctttcag
      601 tggaagaagt agaaaaacga gctgtaaaac ataatttggt ttgcgagaca agggaacaag
      661 taattaaaga aataaaatca ttatctgaag atatggtaaa atatcagaag gtgtttaatt
      721 gctacaagaa atatacacaa gaatcctatg taatgccact taatatccaa acgattagta
      781 aaaatagaat tagaagaatt aacaagtttg atccttctgg aaatatgaag gcacctggaa
      841 atgtcctcaa tgaagtagat gaaagtttaa aaaaatattt tacaaagtaa acgatatttt
      901 tgttgacatt tttctgttcg ttagttacca tgtagttata gaaggacgta agcgcctatt
      961 tttaatgtac acatcgggac gtatgcgccc acatagaaac atagttgaga gacatctata
     1021 tggtgtctct ttttttatta atatgcaaaa aagtctagtt ttttcaataa tatttatgta
     1081 ataataaatt agccaccaca acaaagaatg aaacccatta ttatctagtc tatgtccatt
     1141 ctttttgttt gcagtagttg tgatggcttt ccgtactctt agctcagttg gttagagcag
     1201 acggctcata accgtccggt cgtaggctcg agtcctacag ggtacatact ctaagtgatt
     1261 taaattgcga ataaaatata aaaatgactc cccttttgac tcccccaagc agtatatgga
     1321 tgtctctaaa agttgttttt ttagataata aaaagctgat aataagcgaa aaacacttaa
     1381 aatcagcttt aaaccctatt ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt
     1441 caaatcctgt accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca
     1501 agtaataaga acggccaaac aaaaatagct tttcctattt tttcgtttgg ttgggcttat
     1561 taccgcagat gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt
     1621 ttgtcacagc cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt
     1681 actatttttt ataaatgcta gtaaatcaat cttctttggc cttttttttg ataaaaaaaa
     1741 atactagatg taagataaaa gtgtaaaaac gatttgtttt aggagggaaa acgatgtgta
     1801 ccgcaatcac ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt
     1861 cttataatga agtagtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa
     1921 aaaatttaga taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac
     1981 tctattatga ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca
     2041 atgctgatta taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc
     2101 catggatact agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca
     2161 atctagtcaa tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag
     2221 ctgataaaga aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata
     2281 atcctgtcgg tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca
     2341 attatcgctc gctatcaaat ggaacaccag aaaatcattt ttcaaaccag atcagcttga
     2401 atgtttatag tcgtgggatg ggaggattgg gcttacctgg agatctctct tctgtttctc
     2461 gatttgtaaa agccacattt actaaaatga atgctgcatc aggtgattca gaatctgaaa
     2521 gtatcagtca attttttcat atactaggct cagtcgagca acaaaaagga gtatgcgata
     2581 ctggagaagg aaaatacgaa tatacgattt attcttcttg ctgtaacgtt gataaaggga
     2641 tttattatta tcgtacgtat gaagacagtc aaattactgc tgtaaatata aataaagaag
     2701 acttggatcg tcatgaattg gtcagttatc cagtcataaa agagcaacag ataaactatg
     2761 tcaattaatt aatgaataga tccgtttggt atcatatttt ctgatacagt cataattaga
     2821 cacgatcttt aggtcgtgtc tttttttgct gttttttttg gaaatagcat ccactaatat
     2881 ttttttaaca cgaagagaat aaggtagcct caaaaaagaa aacatggtaa attagatatt
     2941 gggactttat tttctgtaaa aacagaaact ttcatctttt gttttcacgt gacgaaattg
     3001 taagagagaa aatattcgtc cgctatttat tctatgataa gatgcatatg aatgaaataa
     3061 acagtaattt tatattagga tgtgaaagtt aaatgtgcgg tattgtaggt tttgttaacg
     3121 acaaggacaa caaaaaaaca attattaata caatgatgga tcgaatcgtt caccgtggac
     3181 ctaatagttc aggagaatat atcgataaac atgttgcttt aggattcaga agattaagta
     3241 ttattgactt agagggcggt actcagccta tttacaatga agatcggaca aagatcatta
     3301 tttttaatgg cgaaatctat aattaccagc ctttgagaga agaattgatt gcagctggcc
     3361 acgtttttca aacacatgca gatacagaag tccttttgca tggttacgaa gaatggggaa
     3421 cagaattatt gcaaaagatc cgcggaatgt ttgcttttgc tatttgggat aatgaaaaga
     3481 atgagttatt cggtgcaaga gaccactttg ggatcaaacc atattattat gcagaaatga
     3541 acggaacatt tatgtttggt tcagaaatca aaagttttct gcctcatcct gattttaaca
     3601 aagaattgaa caaagaagcg ttgaaacctt atatgacttt ccaatattct ccattaaacg
     3661 gcgaaacatt cttcaaagat gtctatcgtt tgcctgaagg ccattattat acgtataaag
     3721 atggaaaact agacattcag caatattggg atgcagattt tgaaacaaaa gaaacgcatt
     3781 cccgacaaga atggatcgaa aaaatcgacg aaaccgttca agcttctatt gaagcacata
     3841 cggtcagtga tgtagaagtt ggttctttct tatccagcgg ggttgattcc agttatgtaa
     3901 cctctgtatt gaaaccagat cattctttct caatcgggtt tgatgataaa acctataatg
     3961 aagcgattga agccagaaaa ctaaccgaac tattggactt agataataca gcagcagtga
     4021 tcgacggaga catgtcgttc aaagcattcc ctttgatcca gtatcactta gatgaacctg
     4081 attcaaatcc ttcttgcgta cctttgtact ttttagcaaa tcttgcttca caaagtgtgc
     4141 gggtagtcca atctggagaa ggggcggatg aactgtttgc gggataccaa acgtatggat
     4201 tccatacgaa ttcaaaattc atccgtgtga ttgctcaagg attgaaaaaa ttaccaaaag
     4261 gaacacgcta taatttagga cgtaaaatcg gaaaaatgaa aaatttccat ggacggatcc
     4321 atttatatga atcacttgct cctgcaaaag aatattttat cggtcatgct cgagtgttcg
     4381 aagaatcaga agcatcagaa gtgttaacac caaaatatca aacagcacca tctgtggatg
     4441 aaatcatgac tgttcattac gagaaaacag aaggaatcaa agatgaagtc aataagatgc
     4501 aatatgtcga tcttcatcaa tggatgccaa aagatatctt gttaaaggca gataaacttt
     4561 ctatggcaag ttctttggaa gtccgagtac ctttattgga tatcgaagtc atgaaattgg
     4621 ctcaacaaat accaagtaag tttttattga atcaaaacaa cacaaaagat attttcagac
     4681 aagctgcgaa caaacatctt ccggaagaat ggtcgaatcg tgtgaaatta ggattccctg
     4741 tgccaatcaa agcttggttg aaagaagaac acggctacga acaagtcaaa gcattattcg
     4801 aagcagattt tgcaaaagaa ttttttgatc aagaaaaaat catgaagttg ctagatgacc
     4861 atcacgaagg acgtaaagaa gagcaaagaa aaatctggac aatcttcagt ttcttgacat
     4921 ggtataaagt ctactttgta gatgaaacga ttccgcaagc agaagcaatc gactatgtga
     4981 ctgtttgatg aaaatcaaat atcaataaaa aagaggtttt ctcctaccgt ttaacactga
     5041 gtgataagaa acaatttcaa aaaacagctc ttcccgtttt atagatatta tgacttatgg
     5101 ttcggagttg gcagaggaac accattgttt cgaacataag ggggttgtga cacgattttc
     5161 gtcacaaccc ctttacatat gttcaataat cgatttcttt ttcttttgtc ccatcttttt
     5221 tataaatgaa caaagttgat tctttgtttt gtgctacttg ctttcctttt tcgattgctt
     5281 ctttttttgt atcaaaatgg ttgcttgcct tttttgcccc tttagatttc acgatccatt
     5341 gttcttcctc atattctacg atcacatctg aatcaagcag ttttccagca cgaggattcg
     5401 tatcgtgttc atccgtcttg cttggatttt tttctttttc gaaagttcgt ttttctgatt
     5461 cactagcatc ttcataccat ttttttgctt gatcaatggc aattgggatc gcacggttat
     5521 cagggtaccc ttcatctaat aatgcatttg cgatgtcgat tgcttttttt cttgtcaatt
     5581 tatctaagtt tttcattgat gcaggaaagt ccttcatgtt ccatggcata ttagtatccc
     5641 cctttattaa aaaacataat ctatataaac aagtgtaagg gtatttatac aaaaagaaaa
     5701 agattttgtt tgagaagtaa aaccgttgta tttatcgtca agtagtttta cttaacaaaa
     5761 tcgtgaaaaa cactagcaat ctagaaaaaa tcatggtaag ataatcaagt ctgagagtca
     5821 tcttagagga ttcttgtaaa aatgggaggg aaaaacatgc gcgtaaacat cactttagag
     5881 tgtacttctt gtaaagaacg taactatcta acaagcaaaa acaaacgtaa caatcctgat
     5941 cgtttggaaa aacaaaaata ttgcccacgc gaacgcaaag ttactttgca ccgtgaaaca
     6001 aaataatgtt tttaaagttc tcaattcctt gctggtcaaa gaattgagac tttttttatc
     6061 tcaaaacatc taaaatcata aggagcggat aatatgttta tttccatgtg ggcacaagat
     6121 aaaaatggtc tgatcggcaa aaatggtttg ttaccatggc gattgccaaa tgatatgcgt
     6181 ttctttcgcg aacatacaat ggatcgaatt ttagtgatgg gaagaaagac ctatgaagga
     6241 atgggggatc tttcacttcc ttatcgccat atcatcgtgt tgacaacgca accaaatttt
     6301 cgcacgaaag aaaatgcgga agtaatgcat tcgatcgatg aacttttgtc gtatagtcaa
     6361 acgatctcag aagatatcta tgtttcgggt ggtagtcgga tttttcaaga acttcttcct
     6421 tacactggtc ggatctggcg aacattgatc gatagcacgt ttgaaggcga tacgtatatt
     6481 gggaatattg atttttcaga cttcgccctt atagaggagt atgaggggat aacggatcaa
     6541 gaaaatctat atgcccatcg ttttcaaaaa tgggaaagag taaaaaatag ggttgcttca
     6601 aaagaagaat aaaatctatg cgtctagttt gttaataaga aaaaaagcat ctcacgacta
     6661 atcgtgaaga ttcttttttt agttaaaatc aatttagttt ttctttgata tcattgacca
     6721 tttcctgact ggcaataggt ccgttatata gccaactaga ttcaggtccg aattgcagta
     6781 catgattgtc tgtcactgct ttaagattct tccagcttgg ttcattgaac atggcagcat
     6841 ctttgtcact gttcacaagg atgatgtagt cagcatctaa ttcagctagc ttttcagagg
     6901 aaacagcaga ccagtcagaa gtggcttttt tggagacctc ttccactaga tcaggaacac
     6961 caaattccag atcgtcgtat acgattcttc cacttgaccg atttttgctg accataaagg
     7021 ctgagttgtt ggtcacccac aacactgcag ctgtcttgtt ctcgatttta tctgctagtt
     7081 cttctcgagt tttcttgatg gatttttgat aatcggaaat gattttttct gcctcttttt
     7141 cttttgccaa agctttgccg acatctttta gttgttcttc ccatgtgaca tctgttccgt
     7201 tttttacaac ataagttggc gcgattttgc tatattgttc gtatttcccg ccttcgacag
     7261 tagcggaaga agaaatcaac agcaaatcag gctcaaagct taacactttc tcataaggaa
     7321 ggtcataaga aatcgttggg acatccttta aatcatcttg cagataatct tgaatggaac
     7381 cactgccaac agtccactgg gcgataggtt tttcccctag tgcaatcaga tagtcttcta
     7441 agtaactgcc gatgatccgt ttgggcgcgt ttggaatctc tactttatgt ccaagtgtat
     7501 ctgtgactgt atgagtacta ttggatgaag tcgtcgaatt ggcagttgta gctgtttctt
     7561 tatctgaaga gggggagcaa gcgctcagta ctccagccag cagcaatatg ctagataatg
     7621 tgaatacttt tgtttttaat ttcatttctt tcctcctaac ataattgaga atctttctca
     7681 ttggttatta ttcctgatat ttcgaacgct gtcaaccgct tcatttggct atagatatga
     7741 aagtagcgct tgacgaaatg ttgaaacagc gtcataattg agaatgattt tcagcgaaag
     7801 gatgggtgaa atgacgagat taagagcagc tgacattgca gccggctacg aaaacaaaaa
     7861 aatcattgaa aacatgtctg ttacgattcc tgaagggaaa atcactagtt tgattgggcc
     7921 aaatggaagt ggaaaatcta ctttattgaa aagttttacc cgtatactac ctgtgaaatc
     7981 aggaaagatc attgtcaata actctgatat ctctacatac agttcaaaag aacttgcaaa
     8041 aaaaattaca ttattagcac aaactagtga acaccctaca ggtatgacag tagaagaagt
     8101 agtatcttac ggacgatacc cttatcaaaa actgttcagt ggcatggaca cgcaagatca
     8161 tgaagcaatc gagtgggcgt tggatgctac aggtctagct ggatttcgta tgcgggatat
     8221 cgcaacgctg tctggtggac aaacccagcg tgtctggatc gctatggcgc ttgctcagga
     8281 aacagatatc ttgatcttgg atgaaccgac aacattttta gatccggcac accaattaga
     8341 gatcctacag ttattgactg aaatcaatca aatgaaacaa acaacgatct tgatgtcgat
     8401 tcatgacatt aatcatgctt cacgtttttc agattattta ttagcaatga aaaacggtaa
     8461 gttgatcaca gaaggtacac ctgaagaagt gatcacaaaa gagtgtatga aaaaaatatt
     8521 tgagattgat gcaacgattg tcacgcttcc aagaacaaat aaaccagttt ttctaaccta
     8581 tgatttgttg ccaaaagagc aggaggatca attatgagga ctatgaagaa aaatagactc
     8641 ctgcttttgg tctgttttgt tcttttgatt attttttttg ttgctctttc aaatggcgca
     8701 gcgactgtgg gaaaagcaga tttttggcag gcgttgtttg attttgatag atcaaaacaa
     8761 agtcaccaaa tcatacgaaa tattcgtttg cctcgtgtgg cgggagcatt tcttgtaggg
     8821 agttgttttg cattgagcgg tgcattgatg caaggagtga caagaaatcc tttagcagat
     8881 tcaggcttac taggaatcaa cgcgggtgct tcgttggcaa tggcccttag ttttgctttt
     8941 tggcctaaag cttctgattt ttctgtgttt ctaatgtcat tggcaggttc ttttgctgta
     9001 actttgctcg tatttggttt tttgcagttt tcgaaaatgg gcatgaatcc tttacagctg
     9061 attttagctg gcgtagctat tagttctttt tttactgcta tcagccaagc acttacccaa
     9121 ttctttaatc tgcagcagga tcttgctttt tggtttgtag gcggagctgc caatatcaca
     9181 tggaaacaat tagcagtatt gtttccttta tatatacttg ctgttttggt ttctttttca
     9241 ttaggaaaag caatatcttt gattcatttg agtgaaacac atgcattagc tcttggaagt
     9301 catcctaaac gaacgcggtt gattgttttc tgtctagttg cagtgttagc agccattgcc
     9361 gtttcgttag tcggacctgt ttcatttatt gggctgttgg ttccgccagt tgttcgcgga
     9421 atggttggaa cggactatcg ttatgttcta cctgtctctt ttttagttgg cggtggattg
     9481 gttatgttgg cggattttac agcgaggatg atcaatcccc cttttgaaac accttttgga
     9541 ttagtcattg cattgattgg cgttccgttt ttactgatac aagtaaggag ggaacaacgg
     9601 tgaaaaaaat atattggacg ttctatttct tgctttttgt acttgtcatc ctgtctatag
     9661 gtagtttaat gattggtacg ccttttattt acctggatca gctttttgcc acgttcatag
     9721 ggaaagaatc atcttctttg gcgctgattg ttagagaatt tcgagttcct cgattactga
     9781 tcagtttatt agcaggagct tgtcttggcg tttctggtta tatcttgcaa ggagtaacaa
     9841 gaaatgaatt ggcagattct agtatattgg gcattaatgc cggagcagga tttttagtga
     9901 tgctttattt aggtttcttt tcgcactatt cgctgccgtt tctattgcta gttgtagcct
     9961 ttctaggtgg tgttttagct gcttgctgtg tctatcttgc ggcctatgat cgaaatggct
    10021 ttttgggtat gaatcgaatc ttattgtccg gtattgccgt taatgccggc ctttctgcat
    10081 taacattgct atgcacgatc caattatcta aggaaaatta tggatttgtg aatgcatggt
    10141 tagcgggatc aatctggggt gcgagctggc cttatgtttg ggcattgctt ccatgggcgg
    10201 ttatcttaat tccgctagga ggactttatg cacagaggat tggattgcta tcatttggtc
    10261 aagaacgcgc gcaaggatta ggccttaacg tggctaaaag tcaaaaaatc cttttgttgt
    10321 tagctgttgc tttggcaagc ggtagtgtag cggtgacagg tagtttatct tttgtaggac
    10381 tactagctcc tcatgctgcg aagctagtcg tacgtaaaga aaataactgg acctttgttc
    10441 tgagcggttt gttcggttcc atttttctgc ttagtgcaga tatggttgct agagtgattc
    10501 taccaagtgg ggaagtacct acaggaattt tgattgccct ttttggtgcg ccatattttc
    10561 tgtatgcact ttttatcaga caaaaacacg cgctgaacag ttgaaattta ttaaaaaagg
    10621 aaggccaatc atttctaggt cttcttttta ttctatttat gcatttattt gaaagttcga
    10681 ttgctatttt tctttttttc ctataccatt ttatgagaga aggaagggtg agtttttgga
    10741 ccaattgaaa gtaagattac gccggaaagg aatcgaaaag ctgaaaaatc tggccaaaag
    10801 tggcaagaaa aaggaaaaag aacagcgaat ctataaacag ctattcgcca gcgagtactg
    10861 gaagtcagca aaagtgatag ggacgacttt atcgaatgaa tttgaactgg atacacagcc
    10921 ggttatccaa cgagcagaaa aagaacaaaa gaaaatagtt gtacctaaaa ctttgccgaa
    10981 acgtcagatg gcgttttatg aaatggatga acagtccgtt cttgtacgtt cgagttttgg
    11041 tgttcttgag ccacaatcag agaagctcta tttgccggag atgatcgatc tcctcattgt
    11101 accaggtatt atctacaata gagccggtta tcgaatcggt tttggcggtg gttattacga
    11161 ccggtattta aataattttc ataaaaaaac atgtagcctt gtttttcgtg aacaattgaa
    11221 tgaacaatgg caaccggaga gctttgatca gaagatccaa caattgttta ttgactaaaa
    11281 tgaaatggga ggttttcatg aattatcaac agcaaataaa attgcgtgca tggttaagac
    11341 gaccgttatg gacctatgct tttttaggga tacagacagc ggtcttcctc atgatggaat
    11401 tatttccgcg acttgagatc ccttattatg ccggaatgta tggtccttat cttgttcact
    11461 tcaatgaatg gtggcgttta gtgacaccaa tcttcatcca ttttggactg atgcatttcg
    11521 taatgaactc actgatactg tattttatgg ggcagcaaat cgaggcaatc tatggccatt
    11581 ggcgtttttt cttgatttat atgttcagcg ggataatggg aaatacagca agctttgctt
    11641 ttaacgaagc gaatgttctg tctggtggtg cgagtacttc gatattcggt ttgttcggtg
    11701 cgttgtttat cttagggttt cattttaaac ataacccagc gatccagcag ctagtcagac
    11761 attttctttt atttattgtg ttgacatttg tttttggtct tctggatacc tctgtagatg
    11821 tttggggaca tgtcggtggg ctaattggcg gattagtgtt agggaatgtt ttgggtctac
    11881 cgaaacaaca gacaagctat tcgatacacc aaaggattct ttctacacta gtttttgtat
    11941 ttctttttgt aatatgtatt ttactgggtt taaaaaaata tggtttactt gtataatatg
    12001 aaggaagtgc ttaaagatgg agagcttata tgatgtacag cagctgttca aaaaatttgg
    12061 catctttatc tatgtgggtg cacgcatcta tgacattgaa ttaatgacta tcgaattgcg
    12121 taatctgtat gatagccgtt tgattgatca tgatacatac atgacagcgt ggcgcatatt
    12181 aaaaagagag caccgaattg aagaaagccg aaagaaagga aatttgtaat ggacaagaag
    12241 attattggga ttgatttagg tggaacaaca gcaaaatttg caattttaac accagaagga
    12301 gagattcaac aaaaatggag catcgatacc aatattttgg atgatggtaa gcatatcgtt
    12361 cctgaaatca ttgaatcaat caaccatcgt ttgaatttat ataatatgaa agccgaagac
    12421 tttatcggaa tcggcatggg taccccagga agcgtggaca gcgaagctgg aacggttatt
    12481 ggtgcgtata acttaaactg gacagaagtt caatttgtga aaaaactgat cgaggcagga
    12541 acaggaatca aattcgctat cgataatgat gcgaatgttg ctgctttagg tgaacgatgg
    12601 aaaggtgctg gagaaaacga tccagacgtt gtattcgtta ctctaggaac aggtgttggc
    12661 ggtggtatcg ttgctggcgg aaatttgatt cacggcgtga gcggagcagg cggagagatc
    12721 ggccatatca ctgtggatcc tgaagggttc gaatgtactt gcggtaaacg cggatgttta
    12781 gaaactgtgt ctagcgctac tggtgtggta cgtctaggtc gttacttggc agaagaatac
    12841 gcaggagatt caaaactgaa agcaatgtta gacaacggtg aagaagtaac aagtaaagat
    12901 atctttgaaa tggctcaaga agacgatccg tttgcattga tggttgttga tcgcgtatgc
    12961 ttctacttag gtttagcttg cgccaacttg gggaatacat tgaatccgtc aagtattgtg
    13021 ttaggtggag gagtatcggc agcaggagaa tttttacgta gccgtgtaga gaaatatttt
    13081 aaagaattta ctttcccgca agtaagagaa tcaacaaaaa tcaaattagc agagcttggt
    13141 aatgaagctg gtgtcattgg tgctgcctca ttagccttga aatttgctta aaaaaacgga
    13201 ggcattacat ggttttatgg gtaattaata tcattttgct attgattgtg cttgcaatca
    13261 tcttttggga agtctatcta cgagtaatgg ccaagcgttc agcaacgact ttgacagaag
    13321 aagaatttcg agaaggaatg agaaaagcac aagtaatcga tgttcgcgaa aaagatgtgt
    13381 ttgatgctgg gcatatttta ggtgcaagaa acatccctta cactgttttg aaagattcat
    13441 taggatctat tcgaaaagac cagcctgtct atatttacga tcaaaagaaa agcttgagca
    13501 tccgtacagc aaataaatta cgtaaagcag gttatcaaga gatttattta cttaagggtg
    13561 gatatgacgg atggtctgga aaaatcaaaa gtaaaaaagt ataaaacaga tgattgtatg
    13621 acagctttaa aaaccgaatc aatcattaga ttttgaccaa gatcacatga acggcgcaat
    13681 ggatagttgt gccgttcatt ttttttattt tgtgctatta tctacatata taagctaagc
    13741 gaaaaggcag aaagaaggaa acaactatga tcaaattaaa acgtgtttac cttgatgctg
    13801 aaaaagagga tggctataga atactcgtgg ataggttctg gccacgagga atttcaaaag
    13861 aaaaagcgca gttagatctg tggttgaaag aaattgcacc tagtaatgaa gcacgcaaaa
    13921 cttttgacca tgtaccagaa cgatttgctt catttgcaaa gaaataccag caagaattga
    13981 ttttcgaaga agaaaagaag caagctattt ttaaactcag tcaacttatg aaagaacatc
    14041 cagtcattac ttttgtttat ggtgcaaaaa atgaacatca gaaccatgct gttttattga
    14101 aaaaatttat cgaaatggag gcatgcaaat gaaaataggg atcattggtg ccggacctag
    14161 aggattaagt atagcagaaa ggattctcca taataatcag gacagacatt ctcttgaact
    14221 ttttcttttc gatcccaatg gtcctggagg tcgagtttgg cggttgaatc agccaccgga
    14281 gttattaatg aattctgttt ctcaacaagt gacgcttttt acagatgaaa cattgtccag
    14341 tggaggattg gtaacacagg gacccaactt gtttcaatgg agtaaaggac ctgctacaga
    14401 ttatatcgaa cagacggtaa ccaaaaataa gcaactaatt atagaagaaa tcaatcgtct
    14461 ggaaaaagat cagcaatcta ctcgatgttt atacggactc tatcaacgct ggtttttctc
    14521 aaagttgcaa gaagaatttc cagcttctat tcagtttgtt tattctttag taaaaaaagt
    14581 cagcaaaaga aaagatggtt atcttttaca gacagatcag caaagctatt ctatcgatca
    14641 gcttgtttta gcaagcggtc actgggaaaa tgatgagaca gaagaagaaa gagtgtgggt
    14701 agactacgca aagaaaaatg atttactgta tcagcttcct tctaatcctg cagatgtcga
    14761 tctttcgaag ataccgccta aagaaaatgt ctttttaaga ggtctaggct taagtttttt
    14821 tgattatgtc ggattgttta cagtatcaag agaagggcgc ttcgagaacg aaaaaggaaa
    14881 acttgtctat catccatcag gcaaggaacc gattgtatac agtggatcaa gaagggggct
    14941 cccttattac cctcgaggaa gaaaccaaaa gcaaggcgga tcaatggcat ggccacggtt
    15001 actaacaaaa gaaaacctgg agcaatggca tcagtcggga tttttgactg gcgaaacatt
    15061 ttttgactat ctgaaaaaag acgttgagtt gttttattac aagaaaatca tcgaagaata
    15121 tcatttgccg attccaaaga aaaattttga gcatgatttt ctctccctct cttcagagga
    15181 gtgtctcgga aagcatccag cactaatccc ttatagatgg gattggtcgt atttagaaat
    15241 tcctcttacc tatcaggatg agtcctttgc agaagctagc cgagcattta ttgaagggca
    15301 gataaaagaa gcggaaaaag gcaattgcac aggagcattt gcttcaacat ttgatgcatt
    15361 aaaagactgg cgtgatccaa tccggcaggc tattgaatgg cacgtttttt ctgcaaagga
    15421 atacaaagaa atactatggg gatggtttac tcatctaaat agctttttaa cgatcggtcc
    15481 gccagtcatt cggacgaaag aattagcagc actgatagat gcgggtattt ttcatttgtt
    15541 cgaaccgcca tttgatattc agcaaaagca caaccgctat gttatttctc tacaagataa
    15601 agaaatcagc agtcgctatt tgattgaagc tcgattgcca caaacaagct tttctcgttc
    15661 aagtaatcct gtcataaaaa atttgaagga aaacaaactt gttcgccctt tttcattttc
    15721 agataataca ggaagttatc attcagaagc tatagatgtt gagcgaggta cgaatcgcgt
    15781 gattgatgct tcaggggaaa tccagtctga cttttattgc tatggaatac ctgttgaagg
    15841 tgtggattgg ctaactgcag cagtagctag accttataca gatccatgga atctgagaca
    15901 ggcagataaa atagcacaac ttatattgac tgacaaataa aaatacgcat gatgacttat
    15961 ctgttctttc acaagtcttc atgcgtgttt ttaaataatt attaacgaga aacgcgtttg
    16021 cgcattgctt ttttacggtt ttcttcgatc attgcttctt tttcgtcaat tggatcgatc
    16081 actgtttttt tcacagaagc gaccaaacca gcaacggcag cggctgttgc cagacttcct
    16141 actaatattc cagatacaaa
//
LOCUS       DAYYGY010000068        15957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000068 DAYYGY010000000
VERSION     DAYYGY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.069"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..533
                     /locus_tag="ACHA52_002135"
     CDS             6..533
                     /locus_tag="ACHA52_002135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8940813.1"
                     /translation="MKTIPDYKKITIISSITLIIFLILILLVVLVPRIFGLTPYIIED
                     KEMSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKA
                     DTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNT
                     FVELKKKRRRTQKFL"
     gene            661..1344
                     /locus_tag="ACHA52_002136"
     CDS             661..1344
                     /locus_tag="ACHA52_002136"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8940814.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1653..1726
                     /locus_tag="ACHA52_002137"
     tRNA            1653..1726
                     /locus_tag="ACHA52_002137"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Arg,seq:tct)
     gene            1885..2244
                     /locus_tag="ACHA52_002138"
     CDS             1885..2244
                     /locus_tag="ACHA52_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8940815.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2367..3998
                     /locus_tag="ACHA52_002139"
     CDS             2367..3998
                     /locus_tag="ACHA52_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8940816.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4060..4701)
                     /locus_tag="ACHA52_002140"
     CDS             complement(4060..4701)
                     /locus_tag="ACHA52_002140"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8940817.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4887..4963
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4983..5291
                     /gene="rplU"
                     /locus_tag="ACHA52_002141"
     CDS             4983..5291
                     /gene="rplU"
                     /locus_tag="ACHA52_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8940818.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5318..5662
                     /locus_tag="ACHA52_002142"
     CDS             5318..5662
                     /locus_tag="ACHA52_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8940819.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5675..5968
                     /gene="rpmA"
                     /locus_tag="ACHA52_002143"
     CDS             5675..5968
                     /gene="rpmA"
                     /locus_tag="ACHA52_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8940820.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6402..9209
                     /locus_tag="ACHA52_002144"
     CDS             6402..9209
                     /locus_tag="ACHA52_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8940821.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9400..9891
                     /locus_tag="ACHA52_002145"
     CDS             9400..9891
                     /locus_tag="ACHA52_002145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8940822.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9994..10983
                     /locus_tag="ACHA52_002146"
     CDS             9994..10983
                     /locus_tag="ACHA52_002146"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8940823.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11016..11819
                     /locus_tag="ACHA52_002147"
     CDS             11016..11819
                     /locus_tag="ACHA52_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8940824.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11840..12754
                     /locus_tag="ACHA52_002148"
     CDS             11840..12754
                     /locus_tag="ACHA52_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8940825.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12837..13211
                     /locus_tag="ACHA52_002149"
     CDS             12837..13211
                     /locus_tag="ACHA52_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8940826.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13350..13595
                     /locus_tag="ACHA52_002150"
     CDS             13350..13595
                     /locus_tag="ACHA52_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8940827.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13767..14831
                     /locus_tag="ACHA52_002151"
     CDS             13767..14831
                     /locus_tag="ACHA52_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8940828.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15077..15502
                     /locus_tag="ACHA52_002152"
     CDS             15077..15502
                     /locus_tag="ACHA52_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8940829.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15495..15944
                     /gene="nusB"
                     /locus_tag="ACHA52_002153"
     CDS             15495..15944
                     /gene="nusB"
                     /locus_tag="ACHA52_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8940830.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
BASE COUNT         5104 a         2642 c         3485 g         4726 t
ORIGIN      
        1 agaagttgaa aacgatacct gattacaaaa aaataactat tataagttct ataacgttga
       61 ttatattttt aattcttatt cttctcgtcg tcttggttcc ccgaattttt ggtttgactc
      121 cttatattat tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg
      181 tacgaccgaa aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt
      241 caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt
      301 atacaaaagc tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta
      361 tcggtagtcc catctttcag attccatata ttggactagt cctttcaaaa acggcatttg
      421 catggattca ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga
      481 atacctttgt agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt
      541 aaaaacataa atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac
      601 actcatgcag cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt
      661 atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt
      721 acccagcaaa aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca
      781 gaagtaccag ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt
      841 cagcctgaac gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc
      901 acgacaatcg gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag
      961 aaagctaaaa aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt
     1021 caagcagcag atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt
     1081 gcaaaatcaa aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc
     1141 gttttgatgg tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt
     1201 ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat
     1261 catcccgatt tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag
     1321 gaatcagaaa caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa
     1381 tcgagggctc aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat
     1441 acaggaaatc taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga
     1501 taaccttaga acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc
     1561 caaaataatt cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat
     1621 tatctataat tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat
     1681 ccgccttcta agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta
     1741 gaaactgagg tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa
     1801 ggcaaataaa ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa
     1861 ctcatcagaa aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt
     1921 gcaattgaat ttttttatat catgtatatc gaaacggtga gaactgattc tgacacgaca
     1981 agtagggttt ttaaaatgag taaagaagag ttaagcagaa aatctgttca aacattgttt
     2041 aagaaccaag gagtgtacaa tggactatta ggagtgggac tactttatgg agcttatctt
     2101 tcttccgcat ctaaagaaat cacaagtatg ctattaatat ctattttttt tgttgcatta
     2161 tatggcagtt tgactagcga tcgttggatt ttactcaaac aaggtgggct agctctgttg
     2221 gcattgcttt ctattttctt ataaatatag aaaaaataag aatttatcta acaggaaact
     2281 ttgtgaaaaa tattacttta tctcatgaaa tatgatattc taatgaatgg tattttagaa
     2341 aatttcatga aaaaggagtt agataggtgt tcaaaaatta tgtttcctca ctaaaaaaag
     2401 aattttcagg ttatgatctt tcaagatttc agaaagatct tttggctggg atcacagtag
     2461 ctgctgtagc attgccttta gccttggctt ttggggtatc aagcggagct gatgcggccg
     2521 caggtttgat tacggctatc attgccggta tcgttattgg cggtttatca ggcggttttt
     2581 atcaaatatc aggtccgaca ggtgcgatgg cagctatctt gatgtctatt gcggcaacaa
     2641 agggaatgca aggtgtattg cttgctactt ttttagcggg tctattttta cttgcagcag
     2701 gacttttccg attaggaaca ctgacttctt ttatcccagc acccgtaatt acggggttta
     2761 cttcaggtat tgctatcatt attgcattag gtcagatcga taatttattt ggtgttcact
     2821 cggaaggtac aaacgttatt gaaaaactaa gtagttatca aacgatagga tttccgataa
     2881 acacagcctc tttgatcatg ggagggctag tcattttagg gatgtttatc tatccaaaaa
     2941 aatgggcgaa acgaatgcct agttcgctgc tggctatcat tttggcaaca gcagttatgc
     3001 tattgttcca tttaccagta gcaactgtgg gtaagattcc acaaacattg atcagtagta
     3061 atcgtttgaa tatgggagat tttagctttt ctgccttgca acaagtggca gttccagcta
     3121 ttagcattgc tttattagga atgattgaaa gcttgctatg tggtgcttcg gctggccgaa
     3181 tggcaaataa gcccttagat agcaatcaag aattaatggc gcaaggaatt ggaaatctgt
     3241 tgcttccttt ctttggcggg attcctgcta ctgcggccat cgcccgtaca agcgtggcca
     3301 tcaaatctgg tgcacaaact agattagctg gtatgataca tgcagttgtc ttactgctct
     3361 ccatgcttgt ttttgcgcca gtgatgtcac agattccaat gccagcactt gcaggtgttt
     3421 tgattgtgac ggcttggcgt atgaatgaat gggaaacgat caaggagcta tttactaata
     3481 aatattggtc agcagttcta ttgtttatcc ttacgatgat ttgtacagtt atctttgatt
     3541 taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt gtcaaaagcg
     3601 ctgctatcac gatttcatta gaaacgatcg attggcaaag aatggggctg ccagaaacgg
     3661 aaaagctgaa taactggaca gtagtgtata ttagtggtcc actgtttttt atgtcttcag
     3721 aaaaactcaa aaataaattg gaagaactaa atcatcacga tggtgtcatt ttctccatgc
     3781 gaggcgtacc aagtattgat ttgacggcac gtgcagtttt tgatgaattt caagaaaaag
     3841 cagagcaaag aggacaaacg attatctata cttccttgca gccagaagta gaaaaacaac
     3901 tttctcactt gtgggaacat acattcacgc atcaacatca aacagttgct catgcgttga
     3961 cagctttaca tgcgaaatat actgcaatta gccattaaaa caatgaaaaa gaacagacaa
     4021 gactgtaaac tgaagatttt atggtcttgt ctgttctttt taatattgct gtaaaagtga
     4081 agctagtact tgccagtctt gatggataac aggtacaagg gagcgattgt agaaaatagc
     4141 tgctggatga aaaagtggaa gaattcgata gtttttttct gaccattcgt aggcatcctt
     4201 ttgttctgtt aattgtaaga caggccctgt atagagttga ccatgatttt tggtaaccgt
     4261 atattctttg ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa ttaattttgg
     4321 ctggaccttc atcagttcgt aatccaatat tggtgcatgc gcaaatactt cggaacgagt
     4381 aggtgtccga tttggataaa cagtttctga ctgttgtgtc cgtttattga tccgatgagt
     4441 cacgcgatac gggcggctcc ttacagcact ggtgatatac acctcttctc gtgttaagcc
     4501 agttgaagaa agtgcctgca tcaattcttt tccagcttga ccgctaaaag gaacatgcgt
     4561 atcaatctct gttttccctg gggcttctcc cacgagcatc agtttaggat gaagaggtcc
     4621 ttgcccagcg ataaatcctt ctacaggacg gccgttcatt cttaaagaag cttcttgtat
     4681 cagttctttt gggtattcca tttatgctcc tcctttttct tttattataa gaataaaaga
     4741 aacacaatgc gactatttta tttggtagaa ctgtgaggat agaaaagcag aagtattgct
     4801 tgaatttttg ttttactttt tgattttaaa tttagttgac taacggttgc tgttctggta
     4861 agctagtact gttgtaattg tggactccac agctacaacc gctcagaaca agtaataagc
     4921 acagcatatg ctgtcacttc ggatggcgag tctaagtata tcagaaggag gtgctttata
     4981 gcatgtacgc aattattaaa actggtggta aacaagtaaa agttgaagtg ggacaagcaa
     5041 tctatgttga aaaacttgac gttgaagcag gagaaaaagt tgtttttgac gaagttatct
     5101 tagtaggcgg agaatctaca aaagtaggcg ctccaactgt tgcaggtgca actgttgaag
     5161 gtactgttga aaaacacggc aaacaaaaga aagtcgttac tttcaaatac aaacctaaaa
     5221 aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt atcgacgcga
     5281 tcaacgctta atagcgtttg agaaagaagg cctatatatg atcaaaggaa catttaagcg
     5341 aaatgacgca ggtcagatcg tttcattcac tttgaccggg catgctgatg ctggtcctta
     5401 tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg tcaacgggat
     5461 tgcctcatta gccggttttg agccgattgt tgaaatgaat gaagaagaag gcggttatct
     5521 ttataccgaa gtcacttcag gaatgacaca agaacaaaac aatattgctg agattctatt
     5581 agaaaatctt ttattaggat tacaatcgat agaagctgaa aactcagaat atatccaaat
     5641 taaaacaatt aatgataaat aggaggtgca gaccatgttg ttatctatga atctacaatt
     5701 atttgcccac aaaaaaggtg gcggttctac atctaacggc cgtgactcag aatcaaaacg
     5761 tctaggtgct aaacgtgctg atggacaaac agttacaggt ggatcaattc tttaccgtca
     5821 acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg acactttatt
     5881 tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa aacaagtgtc
     5941 tgtttatcca gtagctcaag aagcttaatt gaatcttatg attaaagagg ttgtgacaag
     6001 acttttgtca cagccttttt tgtttataaa aataaaagtt gtagtggagg ctacgaagtt
     6061 tactttgtct ttttacactt aatatttttt tgccaatttg gaatcaggat gttttgataa
     6121 ctatcgtttt caattgatgg aagctatatt gtttgctatc agtgaaataa ttatatggta
     6181 aacagtgttc tttttcttgt ttccattata aaaatcgtat cgggattttt cgttctttta
     6241 aattgaacat ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt aaatagttga
     6301 cgaaacagaa ataaatacat agtattatgt tttgaagcct aagtacaata gtctatagaa
     6361 aaggagcgat accattggca aaacgcattg accgaattta tatgtatgtc aaagaaaaaa
     6421 ccgctcattt aacaaaggca gaatatgatc aaggagtaac aacacaggaa attgctgaag
     6481 tgttagggat tcagcgaaca aatagcagta aagatctgaa tcagctagtg cgcgaaggaa
     6541 aactgataaa gacagacgga cgaccagtac gttacatata tcaaacactc gttacgcacg
     6601 taaagccagt tacaaaacat gtagtcagtt acaaagaaac gccggtctct gaagaaaaaa
     6661 atacactcca aatagatact aaggatatct ttgcaaaaat cattggagct aacggcagca
     6721 tgaaaaattc agtggaacag gcaaaagctg cgattttgta tccgccaaga ggattaaatt
     6781 gtttgattac aggacctacc ggatcaggaa agacttattt tgcacacgcg atgtatcatt
     6841 ttgcaaaagc gcatcatgta atagatgagg aaacggaact gattgttttc aattgtgctg
     6901 attatgccaa taaccctgag cttttgatga gtcatttatt tgggtacgca aaaggtgctt
     6961 ttacaggcgc agatgaagaa aaaatgggga ttatcgatca agcggatggc ggtatgctgt
     7021 ttttggatga gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt tactttatgg
     7081 atcatggaac ttacagtcgt cttggggaaa caacgaaatc ccatgaagca aatgtacgga
     7141 tcgtaggtgc gaccacagaa gacccaggtt cttctctatt agaaactttt gttcggcgta
     7201 tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag aaagtcgatt
     7261 tagtaaaaat catgattgcc cacgaagcaa accgcacgca gcgaaagatt tctttgacag
     7321 aagatgtagc aaaagcattg atcggcagtg tgacctatgg aaatatagga caattgaagt
     7381 caaatatcca gttagtatgt gcacgaggat ttctaaatca tatgcaatca cccgaaatct
     7441 cgatcacaat cgatgatttg acagaaggga ttcgtagcgg attgattcaa ttagcaagca
     7501 accgcagtgc aatggcagag ctttctaaaa tattggagcc aaagataact gtagcaccaa
     7561 atgatacgat catgaaaatc caatcggact cctatgagtt accttataat ctatatgata
     7621 tcatcggaga taaagcagct ttactgaagt cggatggttt ggatcaagaa gcaatcaatc
     7681 atttcatttc tacagatatc aacatccatc tgaagtcttt ctataaagac catggttttt
     7741 cttttaatgc agacagcaaa ttagcagaat ttgtagatcc gaaaatcata gaagtgacca
     7801 atcagattta tgtaatggtc aaaagatctt taccatatga gtttcagcaa aactttattt
     7861 atgctatgag cttgcatatc agttcttttt tgaaaaaaat caacttagga gaagaacggc
     7921 atacgaatga taacatccgt gagatggctt ttgattttcc agatgaatat gctgttgcga
     7981 aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga gattccagaa agtgaagact
     8041 attacttgac cgttctactc gtctcgttac gggcaaatca agcaagcggc agaatcggtg
     8101 tagtcgttgc agctcacgga aacagcacag caagcagtat ggtccaagtt gtccaacagt
     8161 tgttagatgc agagaacgta cgagcagtag acatgccttt ggatatggat ccgaaaacag
     8221 cattggaccg aattgaacgc aatgtacaag aagtagatga aggaagcggg gtcatactct
     8281 tagtagatat ggggtccctt gcgtcgttta attcgcaaat tcaaagagac actggaattc
     8341 cagtacgtac tgttgatatg gtaacaactt ctctagtttt ggaaactgta cgaaaggtct
     8401 cagtattagg gacagatcta gatatgttgc atgaatcatt gaaaaatttt cgaggttacg
     8461 cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc tattcataag aaaaaagctg
     8521 ttttggctat ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa ttgatcgaac
     8581 gagcggtaaa caagcgtcaa gaaacagaat tagaagttct agctttgtcg attgtggaag
     8641 tgaaagaaga actgccagtt atccaaaaag aatatcagat catagctact actgggatta
     8701 ctgatttgaa tatccaagcc ccatttattc ctttagaacg ttttattgat caaaatatcg
     8761 aagtcatttt ggatcagttg ctgatggaat cagaattgga ggaaactgaa ttcatctccc
     8821 tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat ctctgataac ttcatcttta
     8881 ttaatggtag taagttaatc gatccaatgt ggcaattttc tacacaaatc tctcagacga
     8941 cgggaatcgg cgatgaagaa tatggattca aaatcaacct agtgatgcac acagcaggaa
     9001 tgatcgaacg tattattcgg aatgaaccat tgactgtgga agaaaacgaa ttgacaaata
     9061 caacgaatga tcctctttat agccaattgg cagcctcagt tgttctgttg gaagatcaga
     9121 taaaagtaaa agtgccgata gaagaaatgt attatctgct acgtctagtc cataatcagt
     9181 tagataaaaa agaatacact gtgccatgaa tacacaaaaa cgatacactt ttatagtgta
     9241 tcgtttttgt gtattggtta agaatccttt aaattcaatt agtgtatcgt aaaaaagttg
     9301 gcacgctttt tgcttatagt agtgtgtgct aggaggtggt tgttcacaca gaaatgatag
     9361 gggtttctgt gaagtagtga ggaagaagga gttttgcaga tggatattcg attggcacgt
     9421 atagatgatc gtttaatcca cgggcaagtc gcaacagttt ggtcgaagca aacaggtgtt
     9481 gaacgtatca ttgtgatcag cgatgtagtc gctcaagata aattgagaaa gtttctacta
     9541 aaagaagctg caccgccggg gatcacagca aatgtaataa ccgtgcagaa aatgattcaa
     9601 atctatcata gtggattatt atcacgagca aaagtgatgc ttttattcac caatccccaa
     9661 gatgttgaag cggttgtccg ctgcggtgta cagttgaagt cgttgaatat cggcggtatg
     9721 agttttactg aaggcaaatc aatgattacc aattttgttt cagtaaatca acaagatatt
     9781 gattcctttc gttacttgaa cagtcaagaa gtggagttag agattcgaaa agtacctgct
     9841 gacaggaaaa tccttttgat ggaactacta gcgaaaatca aaaaggtgta gcaagaatgg
     9901 ttgttggaaa aggtcaaata attctgttgt aatggttagc aattattaat atttttgaat
     9961 ttgttacaac aaaagtaaat aggaggtata aatatggtag ggattatcct agcaagtcat
    10021 ggacaatttg ctgaaggaat cttacaatcc ggttcaatga tctttggcga acaagaaaac
    10081 gtcaaagccg tgattttgaa accaagtgaa ggtccagatg acttgagagc caaattggaa
    10141 gaagcggttg cttcttttga taatcaagac gaagtgttat tcttagttga tttatggggc
    10201 ggaacaccgt tcaatcaatc aaacacatta ttcgaagagc acaaagataa atgggcaatc
    10261 gtcagcggtt tgaatttacc aatgttgatt gaagcctacg catcacgctt ttcaatggaa
    10321 tctgctcatg aaattgcagc acacatcatt gaaacagcaa aagacggtgt aaaagtaaaa
    10381 ccggaagaat tagaaccggc agaagcaccg aaagcagcag ttgaagacgc acaacctaaa
    10441 ggagcacttc cagaaggaac tgtggtcgga gacggaaaaa tcaaatatgt attagcacgt
    10501 gttgactcac gtctgttaca tggacaagtt gcgacagctt ggacaaaagc agtgcaacca
    10561 aaccgtatta tcgttgtttc agatgcagta tcaaaggatg atctgcgtaa gagactaatc
    10621 gaacaagctg ctcctccagg tgtaaaagca aatgttattc ctattagcaa aatgatcgaa
    10681 gtagcaaaag accctcgttt tggaaatacc aaagcattgc ttttatttga aaatcctgaa
    10741 gacgtattga cagctgttga aggtggcgta gacatcaaag aattaaatgt tgggtcaatg
    10801 gcgcattcgg tcggtaaagt cgttgtaagt aaagtattgt ctatgggaca agaagacgtt
    10861 gaagcgtttg agaagcttga acaaaaaggt gtgaaattcg atgtacgtaa agtacctaat
    10921 gattcacgcg ataacatgga tgatattttg aaaaaagcaa aagctgaatt agcaaaagcg
    10981 taataaataa tattaaaatt tataggaggc ttatcatgtc tattatttca ataattttag
    11041 tcgtattagt tgcctttcta gctggtatgg aaggtattct tgatgaattt caatttcatc
    11101 aaccactagt ggcatgtaca ttgatcggtt tagttaccgg taacctcgaa gctggtatcg
    11161 tacttggcgg tacgcttcag atgatcgctt taggttgggc aaatatcggt gccgctgtag
    11221 caccagatgc tgcattagca tcagtagcat cagcaattat tttagtttta ggtggacaag
    11281 gtgttaaagg tgttccttct gcgatcgcga ttgccgttcc tttggctgtt gcagggttgt
    11341 tcttaacaat gatcgtacgt acaatcgctg ttccaatcgt tcatttgatg gatgcggctg
    11401 ctgaagaagg aaacatccgt aaggttgaaa tgtggcacat cattgctgta tgtttacaag
    11461 gggttcgtat cgcggtccca gcagcagcgt tgctatttat cccagcacaa actgtgcaat
    11521 cattccttga atcaatgcca gcatggttaa cagatggtat ggctatcggt gggggaatgg
    11581 tcgtagctgt aggttacgct ttggtaatca acatgatggc tacaaaagaa gtatggccat
    11641 tcttcgttat tggtttcgtt gtagctgcaa tttctcaatt aacattgatt gctctaggtg
    11701 ctttaggtgt tgcattagct cttatttact tgaacctttc taaaatgggt ggttcatcaa
    11761 ataatggagg cggaagcaac tctggtgacc ctttaggcga cattttgaat gactattaat
    11821 cttgaaggag gaaaataaaa tggcagaaga aaaaatcaaa ttatcaaaaa aagatcgttt
    11881 agctgttgcg tggcgctcta ccttcattca aggttcatgg aactacgaac gtatgcaaaa
    11941 cggtggctgg gcattttcta tgattccagc aatcaaaaaa ttatacaaaa caaaagaaga
    12001 acgttctgca gcattaaaac gtcacttgga attctttaat acacacccat acattgcttc
    12061 tccaatttta ggggttacat tggctctgga agaagaacgt gcgaatggcg cgcctgttga
    12121 tgatgtagcg attcaagggg ttaaagtagg tatgatgggt cctttagctg gtgttggtga
    12181 cccagtgttc tggtttacag tacgtccaat gctaggtgca ctaggtgctt cattagctat
    12241 gggaggaaat atccttggac caatcatctt cttcttaggt tggaacatta ttcgttgggc
    12301 attcatgtgg tatacacaag aatttggcta caaagctggt tctaaaatta ctgatgacct
    12361 ttctggcgga ttgttacaag acgtaacaaa aggtgcttca attttaggga tgttcgtatt
    12421 agctgccctt gtagaaagat gggtatcaat caaattctta ccagttgtat caactgtaaa
    12481 attagataaa ggtgcttaca tcgaatggga taaattgcct tcaggtggtg aaggaatcaa
    12541 gagtgcattt gaacaagtta ataatggctt agccctttct cctgaaaagg taacaacatt
    12601 acaagacaac ttagaccaat tgattcctgg tttagctgca ttaggattaa cattgttatg
    12661 tatgtggttg cttaagaaaa aggtttctcc aatcgttatc atccttggtt tattcgtagt
    12721 cggtgttgtt ggacacgtca tcggactttt ataatccata aaaatatagc tcgggcgttg
    12781 tcccgagctt tttttatata gtatactgtt cataaacaaa tatctggagg gagaaaatgg
    12841 ttcaatcaat caatacaaaa gtagacttgg taattgatgc tacttcgttt acgggtctga
    12901 cagattacgg gcaaatcatg ataggtgata atggttttga gttttaccat gcgcgagata
    12961 gacgcaaata tatccaaatt ccatgggaag aagtggatta tgtactagca tcagtcatgc
    13021 taaagggaaa atggattcct cgatatgcca ttcaaacgaa aaaaaatggt acatacacat
    13081 tttcttccaa agaacctaaa aaagtgcttc gtgcgatcag agaatatgta gatgaagata
    13141 gaatggtaca atcattgagt ttctttgatg tagtgaaacg tggtattcgt agtatgttta
    13201 aacgcaagta aaaaataaag aaaacttcta agaggaggtt ttctttattt ttttactttg
    13261 cagaagaaaa tatgaaatag caaaaccaat tatttgtccc ctttaaatcc catatgctaa
    13321 aattgattag caaagaagga ggttatatta tgagttggtt atggacattg attgttggag
    13381 ccgtgatcgg agcaattgca ggtgcgatca ccagtaaagg aaattcgatg ggctgtgtaa
    13441 cgaatatcat tgctggacta gtcggttcaa gtattggaca agcgattttc ggcgaatggg
    13501 gaccaaaagc tgcaggtatg gcacttattc catctatttt aggcgcagtg attttggtag
    13561 cagttgtctc atttttcttt ggaaaacgat cataagtttt tacaaaatag taaactaaaa
    13621 ggagttgcag cgagactgtg actccttttt ggctttattt atttaaaata aaaagaagtt
    13681 ttccggaaat ttttatttaa ctgtcgtgta cgaagaaaag tttgctataa tggaaaaaag
    13741 cgtgcaaaga aagagggagt ttcattatga tgctacgtgt agaaaaattg cgcaaaaaaa
    13801 tgcaagaaga aaatttggat tcatttttgg ttaccagtcc gtacaatctt cgttacttaa
    13861 cgaactttac aggtacgaca ggattagctg ttattacttt ggaaaaagca ttttttatta
    13921 ctgattttcg ttataccgag caggcggcag cgcaggctca agggtttgaa attattaaaa
    13981 atgtcggacc tatttttgat gaggtagcaa acttagtacg caaagaagaa ctgcactcat
    14041 tagcttttga ggaaacaaca gtctcatttt tagaatattc ggttttagaa gaaatcatcg
    14101 atgcggaatt agtacctgtt tcacagatga tcgaagaact cagagaagtg aaagatgaag
    14161 aagaaatcgc tattatcgaa aaagcatgtc atattgcgga tatggcatat gatcacatcc
    14221 tgaaaatgat ccaaccgggc atgactgaga ttgaagtcgc aaatcaacta gacttttata
    14281 tgcgttctct tggcgcttca ggtgtttctt ttgatacgat cgttgctagc ggattgcgtt
    14341 cggctatgcc gcatggtgta gcaagcaaaa aaatcatcga acaaggtgac ttgatcacat
    14401 tggactttgg ttgctattac gaagggtacg tttccgatat gacacgtaca ttcgctattg
    14461 gtgatcctgg ggaaaaatta aaagaaattt atcaaatagt cttagatgct caattagcag
    14521 ttattgatgc tgcaaaacca ggaatgactg gtgtccaatt agatgcagtt gctagagatt
    14581 atatttcaaa acatggttac ggggaagcat ttggacattc tacaggacat gggatcggtt
    14641 tagagatcca cgaaggacca aacgtttctt ctcgtgcaga aaagcaattt gtaccaggaa
    14701 atgtgatcac tgatgaacca gggatctact taccaggaat cggtggagta cgtattgagg
    14761 atgacttgtt gatcacagaa gatggaaatc gtgtgttgac tcgttcacca aaagaattga
    14821 tcatcttata gtttcatcaa tagaagaagt aagcgtaaaa atcagtgttc acagactttg
    14881 ttatagaaaa tcactcgatt taatgataca ctatagaaga acttttaagc tgtaagagat
    14941 aataaagttg ggtgttttct ccaattcata gcagtttgca gctgatcggc ggcagttttc
    15001 ttaccacgtg atcactatgc cgcatcatag agaattcgga tgtatctcgt gcttaggcaa
    15061 agggaggaat aaataaatgg cagatgaaaa aaatttgatt ttaaacgcaa accaagattt
    15121 aggagagatc gtgatcgctc ctgaagtcat cgaagtaatt atcgggattg cagcttctaa
    15181 agtagaaggc gtatacggaa tgcgcggaac ttttgccagc aatgtcactg aattattagg
    15241 acgtgcagct cacggaaaag gtgtttactt agcaaacgag gaagatggct tgaaagtaga
    15301 tctttactgt tatctagaat atggtgtttc agttcctaaa gtagcaatgg aaatgcagga
    15361 acgggtcagc cagcaagtcc ttttcatgac ggatatcgat ttatctgaag tcaatatcca
    15421 tgtagtagct gttgttcctg aaaaattaga acaaccaaca atcgaagaac tttttgaaga
    15481 cgaagaggaa gagaatgaat aaagaattat caagacacga gattcgggaa atggcattgc
    15541 aggctttatt tccattagac ttcaatgcag acctaacaaa agaagatgcg attttcaacg
    15601 caatcgaatt agaccaccgc gacatgatca acgaagatga atcagagttt gttcctgttt
    15661 atcttgatac gctagttggc ggtgtctgtg caaaaaaaga tgaattggat aaagtgattg
    15721 aaaaacattt aaaaaacaat tggcacatca atcggatctc aaaaatggat ttggtgatct
    15781 tgcgtatcgc gatttttgaa atgacttatg tgacagatgt accagcggct gttgctttaa
    15841 atgaagcaat cgagttggca aaaacattta gtgatgaccg ttcacgtaaa ttcgtcaacg
    15901 gtgttttatc caatgtattg aaagaattag aagctggggc gtaagtccta gcttttt
//
LOCUS       DAYYGY010000069        14824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000069 DAYYGY010000000
VERSION     DAYYGY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.070"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..400)
                     /locus_tag="ACHA52_002154"
     CDS             complement(101..400)
                     /locus_tag="ACHA52_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940831.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(403..564)
                     /locus_tag="ACHA52_002155"
     CDS             complement(403..564)
                     /locus_tag="ACHA52_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8940832.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(579..938)
                     /locus_tag="ACHA52_002156"
     CDS             complement(579..938)
                     /locus_tag="ACHA52_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8940833.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(935..1786)
                     /locus_tag="ACHA52_002157"
     CDS             complement(935..1786)
                     /locus_tag="ACHA52_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8940834.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1803..2636)
                     /locus_tag="ACHA52_002158"
     CDS             complement(1803..2636)
                     /locus_tag="ACHA52_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8940835.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(2643..3329)
                     /locus_tag="ACHA52_002159"
     CDS             complement(2643..3329)
                     /locus_tag="ACHA52_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8940836.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3335..4081)
                     /locus_tag="ACHA52_002160"
     CDS             complement(3335..4081)
                     /locus_tag="ACHA52_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8940837.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(4074..4415)
                     /locus_tag="ACHA52_002161"
     CDS             complement(4074..4415)
                     /locus_tag="ACHA52_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940838.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(4473..4601)
                     /locus_tag="ACHA52_002162"
     CDS             complement(4473..4601)
                     /locus_tag="ACHA52_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940839.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(4594..5301)
                     /locus_tag="ACHA52_002163"
     CDS             complement(4594..5301)
                     /locus_tag="ACHA52_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFM8940840.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            5346..5825
                     /locus_tag="ACHA52_002164"
     CDS             5346..5825
                     /locus_tag="ACHA52_002164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940841.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(5884..6054)
                     /locus_tag="ACHA52_002165"
     CDS             complement(5884..6054)
                     /locus_tag="ACHA52_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940842.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYRAKKPVGRD"
     gene            complement(6066..6332)
                     /locus_tag="ACHA52_002166"
     CDS             complement(6066..6332)
                     /locus_tag="ACHA52_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940843.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(6343..6534)
                     /locus_tag="ACHA52_002167"
     CDS             complement(6343..6534)
                     /locus_tag="ACHA52_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940844.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            6825..7247
                     /locus_tag="ACHA52_002168"
     CDS             6825..7247
                     /locus_tag="ACHA52_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8940845.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7264..7686
                     /locus_tag="ACHA52_002169"
     CDS             7264..7686
                     /locus_tag="ACHA52_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8940846.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            7715..7915
                     /locus_tag="ACHA52_002170"
     CDS             7715..7915
                     /locus_tag="ACHA52_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940847.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            8002..8451
                     /locus_tag="ACHA52_002171"
     CDS             8002..8451
                     /locus_tag="ACHA52_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFM8940848.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            8753..8890
                     /locus_tag="ACHA52_002172"
     CDS             8753..8890
                     /locus_tag="ACHA52_002172"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8940849.1"
                     /translation="MTLQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRV
                     K"
     gene            9028..10233
                     /locus_tag="ACHA52_002173"
     CDS             9028..10233
                     /locus_tag="ACHA52_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8940850.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(10285..10372)
                     /locus_tag="ACHA52_002174"
     tRNA            complement(10285..10372)
                     /locus_tag="ACHA52_002174"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10338..10340),aa:Ser,seq:cga)
     gene            complement(10517..11083)
                     /locus_tag="ACHA52_002175"
     CDS             complement(10517..11083)
                     /locus_tag="ACHA52_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8940851.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            11338..12669
                     /locus_tag="ACHA52_002176"
     CDS             11338..12669
                     /locus_tag="ACHA52_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8940852.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            12635..12985
                     /locus_tag="ACHA52_002177"
     CDS             12635..12985
                     /locus_tag="ACHA52_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940853.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(13077..13496)
                     /locus_tag="ACHA52_002178"
     CDS             complement(13077..13496)
                     /locus_tag="ACHA52_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8940854.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(13497..13841)
                     /locus_tag="ACHA52_002179"
     CDS             complement(13497..13841)
                     /locus_tag="ACHA52_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8940855.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            14191..14787
                     /locus_tag="ACHA52_002180"
     CDS             14191..14787
                     /locus_tag="ACHA52_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8940856.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         4387 a         2817 c         2421 g         5199 t
ORIGIN      
        1 aacttaacta attccgctcg ttcttttgct tttttatctt tctgaatctt catcatgatt
       61 ttcttgagga tgatatcact gtattgtgta atgagatcca ttatttctcc tccacatacc
      121 taaactgtcg cccctttgaa tcaatccata agctcctagc tctatcccag atgatgtttt
      181 tgctcagtcc agtaatttca gataactgtt cagctgtacc cgttactaga attcgatcac
      241 catgccagat tgcaattttt ctcggcgttt tccgtttagg cttttcagtc cacattgatt
      301 taccgagctt ttggacttct gcaactattt ctttgtcttc ttgccaattc tcagaatgtg
      361 tcagttcaat gattcgtttc attgctgctt tcttacccac gctcattcct ccaatctacg
      421 aatttccctt cttaaattca ctatgtgcaa attgattgcc tttctcgccg tttcattgac
      481 catcactgcc tttatccgtt ccagatcgtc aatctcacgt tgaaggcttc gaatacgcat
      541 ttgaatcact tcttctgttg tcatgatgga ccacctcgtt aaaaacgctc ttccttgaac
      601 gtattccgat attttttagc taatatcaac ggcacttgat attgatgaca gaacaacttt
      661 gccttgatct taaagtcttt tgtctgcatt cctttgacat ctacgacttt gacaagtttg
      721 ccgtttttat aaaatgtgaa gtcgggaata tactcgatct tgcgatactt ttttccatct
      781 agttcaaatt tcggcatcag ctcaaatctt tcctgaagtt ttactttcca gccgttcgct
      841 tctgcttgcc acaaggctaa atcgtaatac tctgcttccg cgatagaatc aaacttgata
      901 cctcgatgaa cagttttctt attacggtat ttattcatgc gatactacct ttcactggtt
      961 ttatgcgctt gtctgctgtt tgttggaatt tcagcgcata accttctgaa ttcttaaata
     1021 ttctagaaac aattctttcg ccgtaggctt ctcttagttc aggaccggat aagtttgttg
     1081 tgatgatcgt tgccttgttc tgtctggctt ctaagagcgt gtttaacgtg ttgtttgtaa
     1141 actgcctact atttgatacc ccgctaccta attcagctcc aatatcgtca aaaaccacca
     1201 aatcagttgt tttgatatcg gctataagcg atccttcaat ttcttttctc agttcagcat
     1261 tgttataaga aaactttatt tgctctaata actcttgata gcttataaaa agtattttct
     1321 tgtcataatt tgagcgctca agtatttccc aagctgtcgc cattgacaag tggctttttc
     1381 cgcttcctga tttccctgat agaatgaaat gtgcaggatg gttcagtaag acatcattta
     1441 caaagctttt agctctttct aaagcaattt tcgtttcttg gtccactacg tgataattct
     1501 ccattttgca tttaaacaaa gttttatctg ttaataccga accattttga aaaaaactca
     1561 acgctcgtgc ttttaagctg tcgttatata tccgttcggt ctgtatatcc tctttcacac
     1621 gtaacgcttt ataaccacaa ctcatgcatg ttggtttaca acgttctgaa ccatccttat
     1681 ttttagctcg ccaactatac aaaggttcgc tacattctgg acatttcccg ctttgcacta
     1741 atactcttct tattagtttc tccatagcat ttgctaggct ttccatgtga tgcatctcct
     1801 ttttaaattg gcaagtcgtc atattcacta ggattgctgt actgtagttt ttgactttgc
     1861 tttttatgat tattcttgtc tgctttgatt tcgaatttga gcttctcaaa tttttctctc
     1921 aatttcttag cacttctaat atttccaaac caaaattcat ttgtaggtag ccaattgatc
     1981 acatactcaa tcgcttctat agacgcttta tctctttctt ccatcaacct gattgtgtct
     2041 gcccattttt cgatatctac tttgttcatt tcttttggaa aatcttcagt taaattactt
     2101 tgcaattttt tagcaaggcg taagtgttcg tcagaatact tacctttctt ttcttcttta
     2161 tctatatcta tatctttctc tatctctatc tctaactctg gtgtagtttt gtctggacat
     2221 ttgtccgaca cttgtcctcc agttattaaa ttccgttttg cctcttctat tttctttctg
     2281 tattctcttt ttctatctgc ttcagttgag gattttccaa tgaaactttg tatatcagac
     2341 atataaattg ctccgttatc taatacgtca ataagctgca aatcacggaa aatttgtacc
     2401 gctttttcta cgactcctac agaatgtctt gtaatagttg cgagcattgt agagttaaat
     2461 ggaatcctgt cattaaacat caacttacct tcgtgtttta gacttcttaa ataaagtttg
     2521 agaagaatat tagaataaat atagccatct ggcatacttt ctaagagaac catctcgtca
     2581 ctatcgaaaa aattctcttt tagttttaaa tagtagtagc gtttgttgtc agacaatatt
     2641 ttttaccctc ctattctaag tttcttaatt gtttcctggt ttaacttgat ccctttgatt
     2701 tgatatttat ttttgaaatt gatcacacct atattgtgtt tctccgtgtg atgaatcctg
     2761 cagagtgctg caaatgtgta ctctgaatga tcaacttctt tgcgctttcg tcttcctagc
     2821 gctttgtcaa agtgatcgat gtcagctcct gttttgccac agatgcagca gactcttttt
     2881 gtgatgcatt tgtagaagta atattcttga ttcgctggta aaatctcata gccttctttg
     2941 aaaggaatat gatgttcaaa gatgaaatct aagatgatat ttgctaagac attagcatca
     3001 ctcacagttg tattcgattc gtctttgagg cttattttgc gccctgtgac gccttcaaaa
     3061 cggaagtaaa agaattcctt ccagaagtcc gttggcatgc ctgtatcgat gaaaatatcg
     3121 cctatcagcg catagatgaa gtttcgttgc tgcacagtaa atcgacgtgg atcaataaat
     3181 cgaacttcaa taatccgatc gccatcatat ccgtcataca tcgtctttag tcgatcaatg
     3241 ttcacttctt cattgatcgt tgcacctatg tcttttcctt tgaacttttt cagaaccgct
     3301 gaatatgaat cgatcaatgg tttaaacact catatcactt ctcttttgtt tcttctctgt
     3361 actgatcttc aatccagtta acgcctcgtt ttagaatgcc caagtctctc ttggtccatt
     3421 tactgtcatc agcggttata gaagccgcat cagtcaatgc aacaattgct tcatcaattg
     3481 atttttcgta cttgttagca accagttgta aagcatccaa gaatagcttt ttgcttcttt
     3541 gagtagctgg ttcaagcatc gagacatctt ctggcatatc ttcgccagca aatatatata
     3601 gccctagccc aaacattgct agatttttta caagacagcg catgatcgtt ttgttgatat
     3661 caaacatagt tgctgcttca actcgctttt cgatttttcc aacaatctct tttttcttcg
     3721 tttcgttatt ccactgataa tcattgactt cgtaggtata tggctcatct ttcattgcct
     3781 tgtttgcacc atccatgact ggtaaccaca tgtcacgctt tactccgttg actgtgatac
     3841 tggtaaaaac catatagcct gttttcttat caaagacata cggcagatat gtttctggat
     3901 catgatagat ttcataatct actgcctcac acactttgct aacttctgcc caagcccatg
     3961 cccacgaaag ataagtgagg ttatttcgtt tttcagtaac atctttcaca tttattttgt
     4021 ataaggtatt aaaaagcgta ttatcacttc tatttttcaa aggttgttcc ttttcactca
     4081 tcaaattctg cctccatttc agcaatgtat ttcttacctg atccgtaata agagatatca
     4141 atcaagttat ctctgtcata ctcttctagc gcatcaatca agccatcttc gatgacatag
     4201 atatattcag tttttttgga cttcctcgat aaatggataa gatagacatg atcccaaata
     4261 ctcacaaaat ttcccaaatc gtcttgatca catgctagtt cttcatccgt caaaagatta
     4321 cgtctgattt ttcgattgct tgtttccttg atattcgatt tgccccaact aggatcagtc
     4381 aaatattgat ctagagtgga aagttctttt ttcatgtggt aacatctcct tagatgtatt
     4441 ttctttgtga ctcattgctt tggtcggctg agtcactttt ttatttgttg ccatgctttt
     4501 tgcttatcaa tatgttgttg gcttaggatg tttggtttat tgtgtctcca ccagcgatta
     4561 gcaattatta cgcctatttt tagcgcttca gctctattca tcctctaaca actccatttg
     4621 cttaataatt gtcatcgtcg ctgtagaagg catccaattg ttaataaaat cgataacatc
     4681 atcgaactgt tttgccttga tacgatttct agctactgct ccagtgattc gcttaattcc
     4741 gccattcaag tctttaaaca attctgcttt tgctttgcta tttaaatgct gctgattaca
     4801 aataactcta attttgttgc gaaccatatt agaaattgtt cggtaatctg gttcagctag
     4861 aagttgattg ttcttcaaat cgtttaaatc agactcgata gcgtctactc tttcattcgt
     4921 ttcttcgttt gcagctagag caagtaatgc catttctcgt ttagttgctg gaattgatgg
     4981 caattggtct tttaattctt tttccatctt gttgaaggcc tcaatatatt gaagtttaaa
     5041 acttaaagct ttcttaccag tgaagcccat tgctaataga gagaagccgt ctctattcat
     5101 ataaataatt cggtatgatt gtttattttg aggatgaaca taagtatctt cccaaaatag
     5161 gtctgcccaa ttttcggcaa ccccctcttt tagttcatca atagccgtca aaacatgtct
     5221 gtgcttttta tcgaagcttt ctgcaacttg taagctcgta gtcacagctt ctttattttt
     5281 caaaattact aattcttgca ttatttcttc tctccttttt gatataattc tggtggggtg
     5341 aaaacatgag cagaaaaatt gataatataa tattgttaac aactattttt tatttagtaa
     5401 tcggagcaac tctttcctta ctgtcatttt tgtccaacag tagtttgatt gtgttaaatc
     5461 aaaaaagcat attaacagaa tttgcccaca tagattactt ctcttttttt aaaactggac
     5521 taatatgttt atttctatct tcatttatcc tctatatagg aactacattt tgtctaatgg
     5581 tgcttaattt aaaaatgttt ataattgaaa aatggatagt tattatcgca aagattttct
     5641 catcgatatc aattatcatt ccaatgacat tgacgttttc taaagagcaa tttaacgtag
     5701 ctactacatt cgtatctttt atagctctat tttcgtttgc tgttcctaaa acttttcaaa
     5761 gcaaatctag caacgcaaag gttaatgtag aaaacactga tagaaaaaat gatgaaagtt
     5821 attaaaaaaa ataattcttt catttattgt caaacctttc ttatagacat tagacttttt
     5881 tagtcagtcc ctcccgactg gctttttcgc tctgtactca gcttcatcaa gcccaatgaa
     5941 aatccaaagc atgtaaacga tcgtgccgat taatgcttgt ctactacccc atagacctaa
     6001 agcgtagata attagtggcg cgctgaatac taatgttcga ttaaactttc ccatgttgac
     6061 ctcctttaaa ttttgtattt agccatcagt tcatcgatgt cctttttgtc atatcttaaa
     6121 aagccatcta tacgaatttc ttttaaaccg tgagctatta acttctcaaa accttgatcg
     6181 tttacacctc ccacatattt tctagcttcg atctttttta aatatcgtgt ttctgacaaa
     6241 ttcttttttg aaagttgact aagtgcgtcc tttacaactt caacaattgc aattcttaaa
     6301 cgagtcaaaa actcttcgct tagaatattc atgacttgcc tcctatcgaa ttctgtaatc
     6361 acggatgatc tctagaatag ttctgtttgc ctttggtcca ctccagtgtc catcaagaat
     6421 ttgttgcatt cgtacacgcg tgtgaccata agcagtagcc aaatcatcca tagttattcc
     6481 attctctttc ataaactctt tgatcgccgc acgaccgtta tccaagtttg acattaaccc
     6541 acttcctttt tttataaatt tgtaagttaa aatgatagaa aaaatataat ttccattgac
     6601 atttttacac tatagagtaa aatgaataca tagagaaata agcctataac aaaacctttt
     6661 atttgcactt ggtcgccaaa ctttatgcta taaggtgtgt ttttagtttg cttttttcta
     6721 tcaaattaac ttacggataa ataataactc tatatcgtaa aattgtaaag ctatttttac
     6781 tctttttcgt aaattatttt ttcgtgttgg aaaggaacgt tggaatgaca acatttgaaa
     6841 gaataaaaga actagcgaaa aacaagaata aaaaccttca acaagtcgct aaagatttga
     6901 agtacggaga gaattacttt tatagtttaa acgcaggaaa acagccaact gccgaaaagt
     6961 tacaagaaat tgctgactac ttcgacgtat ctgtagatta tttgctagga cgtactgaga
     7021 aaaaaagata ctatgattta acagaaaaag acgagcgtga tatccaaaaa gaattgcagt
     7081 cattaatcga cgatttatct aatgctgatg gaatggcttt ttcgaagaaa gataatgaaa
     7141 tgagtgaagc gacacgtgaa gctctcatta tttctttaga gaatgctttg cgaatagcta
     7201 aaatagaagc taagaaaaaa tacacaccta aaaaatatcg cgattaaaag gagtacgttg
     7261 tttatgtatt tacctcaaat tgataacaaa attaataagc tggtaaaact atatcaaaca
     7321 cgcaatcctt ttagaattgc acgagaattg aagatactag ttttagaaga agatttggga
     7381 gaaatttatg gttactatag taaattcaaa cagataaaaa tgatccacat aaattctagt
     7441 ttagatgaga tgggaaaatg gataacttgt agtcatgaat taggccactg tgttttgcat
     7501 ccgaatgaaa acacacctat gttgtctaaa aaaacgctag tatctgaatt aaaaattgaa
     7561 aaagaagcaa attatttcgc aactcatctg actattgatc caactttaga cggctttgat
     7621 catatgacaa aatatgaaaa gctcgtctgc tttggattac cagatgaatt cgatcgtttc
     7681 ttgtgaatta taatacaacg taaggaggat atagatgaaa ataggaatga gaaaaccaag
     7741 tataaaaaaa tccataagcg ctagaaccac tggaaaagca aaaagaaaag taaaaaaagc
     7801 agttattcct ggttatggga aaaaaggtag cggctggatt aaagacccaa aaaaggctgc
     7861 ttacaataaa gtatacaaga aaactacatt cagtttttgg gatttattta aataaaaaaa
     7921 gcccgtgcag caacacggac tcgaaacctt atttctaaga ttccccaata aaaatcatat
     7981 catagaaatg aggaataaaa aatgaaaaaa gtaataacaa gtttattagt ttttgcttct
     8041 ctgttacttt tcactgcttg tggtaaaaaa gtaactacag aagatttaaa agctaatgat
     8101 tggttgattg aagcaaccaa agatgacgaa cctaatatga tcgtatcatt ttctgatcac
     8161 gtcgtatctt ttagtgttga cacaagttct atgaaatcaa cttcaagtaa cgattgggag
     8221 gctatgggag aagaatttgc taaaagctta gtcgaccaaa tgaattacaa atttgagtat
     8281 acacttgaag gcgatgttat gacttggaaa gatgataaag acaaagataa tgatgccaaa
     8341 tatactgtca gcaaagaaga taaaaatatt attttaactc cagaaaagcc taacaaatct
     8401 gatgataatg agaaattagt tttgaaaccc tacaaaaagg aaaaaaacta aagaatctag
     8461 aacaactact agctcaacaa gtagcaacga acaatcatct tcgactacaa cagaaagtac
     8521 tattgcatct tctagtactg tagaacaagc tgctattgcg cagaatacgc ctcctgaaac
     8581 tagtagtact tactctgctg aaagtgtaga aaactatgca gaagctactc ctagttcaag
     8641 tgttgaacaa gtttcaccca ctcccagttc aacggagcaa tcagtaccac aagattatgt
     8701 accagctaat caagctcaac caacggcacc ttcttcatct gaaaccgaaa ccttgacttt
     8761 gcaacctggt gaagggacca aacaaatagc tgaacgagct ggaattacgg tagatcaact
     8821 gtttgcatta aacggtatgg atccaaacaa ctacatgctt tatccaggac aagaactaag
     8881 agtgaaataa aaaaagcaca tttgccgccc gaccaagaac gcaaatgtgc aaagagctac
     8941 ggtaaagtag gtctatttgt tgtaccctat tttaccatct cgaaatccgc tgtacaaccg
     9001 aacaaacgta cgaaaggaaa gataaagatg gcaaaaaaag aaattgataa acgaattaaa
     9061 aagcacttaa caaaaaaagg cgaagaaaaa tatcagttta gtctgtactt aggagttgat
     9121 cctctaacag gaaaaaaaag aaaaacaacg agacgaggtt ttagtacgcc attagccgct
     9181 gagagagctt taaaaagatt ggaggcagat atacaggaaa aaggactgca agcttctaca
     9241 gctcaaaaga gcaagaaatt cgaatatgtt tacggcttat ggtttgaaaa ttataaaaag
     9301 acagttaaag aaagcacttg gtcctccacc aaacaaattt ttgacacaca catcttaaaa
     9361 gtgtttggag ataagttcat tgacaaaata gacgtttttt tctgtcaaga agcagtaaac
     9421 acttggtccg atagccatcc taaaattttt aagaaaataa aaaattacac atctaatgtt
     9481 tttgattacg ctgcttcttt acaaattata actagcaatc ctatgaaatt agtttctatt
     9541 ccacgtggag aagctctaga tatcgaagat aaaaatatcg aattttatac taaagaagaa
     9601 ttaatcaaat ttttagaagc aataagaaac gatgatgatg aaagatattt attcttttcc
     9661 ctattggctt ttacaggtat aagaaaagga gaagcttttg ctctgacctg gtcggatatt
     9721 gactttaaga gcaaaacact caatatcaat aaaactgtaa caagaggtta tcaaggaaga
     9781 ttgatcgtaa atactcctaa atcaaaatct ggaaaaagaa aaatttatct ggacaatgat
     9841 ttgatcagtt tattgagaaa gtattataca aaaaacaaaa cgatcgttac aatccaaagg
     9901 gaaaacttaa tttttcacca tgatggtctt ctctataacc ctacagtttc acgttcgtgg
     9961 cttaatgtca tatataagca tcatccagaa ttgactaaaa gaatcactac tcatggtttt
    10021 cgacatactc atgcctctct cctttttgaa tcaggagctt ctttaaaaga tgttcaggaa
    10081 cgtttagggc atgcagacat tcaaactact tcaaatattt acactcatgt aactgaaact
    10141 caaaacaaaa aagtcatcaa taattttgta gcatttatga agaacagtac tcagggggag
    10201 tcaaaagggg agtcaaaaaa cattttaggc taaatataca acaaaaaaga cacgatcgaa
    10261 atcgtgtcta atcgttgata tataaggaag gtacaggatt tgaacctgcg cgccggtatt
    10321 agccggttcg ccggatttcg agtccggtgc attaccactc tgccaacctt ccgtaagtac
    10381 tatttttaaa cttttttcac gaaatgtcaa gcctatttct aatagataaa taacagaaat
    10441 cgcttttttt agcaaaaaag tccattgaca gtttgcagcc aacggactta ccgatcaaat
    10501 tcatactgat agaacactat ctgattattt caggattatc taatgctaaa atcgagtcaa
    10561 ttgtccgttt gatcagtgca atgatttgtt cttctgttgg atgcatgatg tttgatgaca
    10621 tcaatgacgc taagccagtc actgctaccc atgtaccagt atgcagagca tcgacatatt
    10681 catccgataa ttgattgtat ctttcatctt ctttaactat ttcacggaaa taattgtatg
    10741 aaaaatcatg cattttcttt ccgtccccat gttcatcaat gtacaatgcc atgtaaagac
    10801 tatgatgctt ttttgcaaag tagatatagt tcaaaccgag atcaatgatt ttatcaccag
    10861 tacgttcaat agggaaaaca tcttctttta gttgttcaaa gatagaatcc agcaacgtat
    10921 tttttaaatc ttccatgttt ttgaattcca gataaatcgg ctgtgtcgaa atccccatct
    10981 ttttagcaat atttctcgca gtaaatcctt taaacccttc ctttgcaaca acatcataag
    11041 cagcttttag aatttgatcc ttggtataga cttttcttct cattaaaact cccccacttc
    11101 tttactgcag tatatgttgt tttattgtac cctattttta gcattttttg caaacatttt
    11161 actcaaaacg ttatatttta aatttttttc agtcttcttt aacttttttt tgcaaaatta
    11221 aaaaaatttg gttaaattca atgggttccc ttacattttt agttttgacc taaatgatag
    11281 ttgtccaaat ctttcatttc aagttatagt taaagaaaat gaaaaagaag gtgtgtaatg
    11341 gaaaacgaaa ctgacgttat ttatattcat cctcaaaaac gaatcgtttc acagaaaaga
    11401 aagtattttt atcttggttt tacaggtgtt ttttttcttt ttattggctt gctttctaat
    11461 actcctactg ataattggag tggattgcta actattctta cctcgccaag caatcttttg
    11521 acagattatt ttgctttagg tgttttcgga agcgcattca taaatgtagg gattttgacc
    11581 ttactttctg tgcttttagc ttatcggcac aaggtgattt taaacggtcc cttatttgct
    11641 tcgattctaa cagttaccgg tttctctttt tttggcaaaa acctttataa ttctatctca
    11701 atcattttag gtgtgtatct atacgctgta tttgtcaata aacctttttc tcagtacatc
    11761 atgatcggac tgtttggtag cgtattatca ccggtcgtta gttatatcac ttttggtatg
    11821 cgttttcctt tactgatagg tattttattg gggaatttag ctggcatagc aattggtata
    11881 cttcttccgc ccttagctgc tcaaacttta gttttccatc gtggattcac actttataat
    11941 attggattca cctctgggtt gattgcgatg acttttactg ctgttttgag gttattcagc
    12001 tattcaatcg tcgaaaatac attggttttc aatgaatatc actttcctct catttggatt
    12061 atttttggct ttttttcgct gacagtcgga attggtttct attataattc ctttcggctt
    12121 tcggggattc gagaaatttt cgattcatct ggaaaattga caactgattt cattgctaat
    12181 tctggtattg gtgctacttt gatcaacatg ggcttagttg gtctgatgct gtcttcttat
    12241 gttttgctgg ttggcggtca gttaaatgga cctgtcatcg gtgctatttt atctgcggtg
    12301 ggtttcagtg cttttggttg tcatttaaaa aacagttttc cgatactagt cggaatattt
    12361 attgcttctt tgtttggaac ttttcatgag atcaccagta ctggcatgct tgttgcagct
    12421 gtgtttggga caggtcttgc gcccataagc ggtttttacg gcagttttta cggtgtaata
    12481 gctggtgtgc tacatattgc tcttgtccat aacgtcagca ctttgcacgg aggactaaat
    12541 ctatataaca gtggtttttc aactggtttc gttgcaggta tccttgtacc gatactagat
    12601 aactttaccg ctgtcagaaa ggaaaaaaag acacttggaa aaagaattat caaaaagaat
    12661 catcgatgag cttcttactt atttatattc ccatgaaata actgaaatac gtattggagt
    12721 agaatttgct gaggaagggc ttttcataga gatacagggt acggcagaaa ctcagccaga
    12781 agatttagat gtacttgctg atttgctgaa caatcctcgg gaccctagta tggaaatcta
    12841 ctatcaagaa ctattaggta atccccgaca tgatcgggaa gattatcatc tgttaggtag
    12901 tttgattgat gacgtggaaa tcatgtatgc ccagccgatt atcaatatta agatctttcg
    12961 acgtgcttct cattcctatt attaacccta ttttctattt ataaaagact gaatcaaaaa
    13021 agcagctcct gaaagttcga ttaaaatcta ctttcaggag ctgttcaagc ttgacattat
    13081 tgattttcca atccttcata cacttcatct gttacaggtt ctaaaaactc tgaattacca
    13141 gcagtgatcg ccaaatgttc aaaccaactg tcttttgtcg caccatgcca atgtttaatc
    13201 cctttgtgcg ttacgacgac atctccagcc attaatcgtc ttgctggctt tccttcttcc
    13261 tgataccagc cttctcctcc ggtcacaagt aagatttgat agccgtctgt atggacatgc
    13321 cagtgattgc gacaacctgg ttcaaaagtt acattaccta caccaacaga aacttcggga
    13381 tcattcacta acatattcag atagctttgc ccggtaaata cttgagcgta agcttcgttt
    13441 ttttcacctg ctggaaaaat cacgccattg tgtatttctt cgtgttttgt catcatttac
    13501 gcctctttct tatcttcgct attttcttct aaatagattt cttttgctaa actaaaagct
    13561 gaccaagctt ttggccattc tacataaaaa gctaaatggg taatgatttc tgctatttct
    13621 tcactagtaa tgccattttg cttgccaatc ttcagatgag ctggcaattg ttcaaaattt
    13681 cctcctgaaa ttaatgcaga tatggtgatg atactacgct gatgagcaga taattcttct
    13741 ctggaccata cttgtccaaa taaaacatca tcatttaatt ctgcaaattt tggtgcaaaa
    13801 tctcctaatt gatctcgtcc tgcggtttgt ttttctgcca tttctcttat tctcccttca
    13861 cgactgttag gtgattcaac tatggttcta aggcaacttt attttctatt gtttcttcgc
    13921 atttcatttt aaatgtagtc tttcagcctg tccaatgctt agtttattca gtcgatatgc
    13981 tttttaagca tagttgctaa cgcttttttt attttaaaaa aaacaagtta ttaaataagt
    14041 ctttagttgg atgttttttt gagatcaatc ttttatactt atgatgaact tacaaaattg
    14101 tttgttttct gtcatatcaa aaaatggcgc agttagttgc ttttctagta aagtatagtt
    14161 aacaaagaac ctctaaggag ttgatgaata atgaatattg ctgcctctcg aaaactgata
    14221 aatttcatat ccattatcgg attaggccta tcaatcgcgc tgacgattta ttttattaat
    14281 ttaggtgttt tcaaagattt gaatgcgtta agaggattag taggtgattc catcatttta
    14341 ggaccgatta tttttgtctt catccaaatc ctgcaagtag ttattccaat cattcctgga
    14401 gggatcagta ctgcagctgg tgtcctgata tttggccctt atgctggatt tatctataac
    14461 tatattggaa tctgcattgg ttcgattatc atttttctgc tagggcgtcg ttatggaaag
    14521 ccattcatac tttccatgat tagcgataag acatataaca aatatattgg ctggctggat
    14581 aatcaaaacc gttttgagaa gctgtttgct ctagctattt ttcttccagt tgctcctgac
    14641 gatgcgttat gtttgatggc tggattgaca aacatgtcag tgaaacgata tacactaatc
    14701 attttgattg ctaaaccact gtcgattttc ttatatagta tggccttgat ttatggaggt
    14761 caatatctaa gtggtcttct tggataatat ttattttgta aaattagtca ttgattgaca
    14821 atgt
//
LOCUS       DAYYGY010000070        14799 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000070 DAYYGY010000000
VERSION     DAYYGY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.071"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1086
                     /locus_tag="ACHA52_002181"
     CDS             <1..1086
                     /locus_tag="ACHA52_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8940857.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1304..2446)
                     /locus_tag="ACHA52_002182"
     CDS             complement(1304..2446)
                     /locus_tag="ACHA52_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8940858.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2451..2636)
                     /locus_tag="ACHA52_002183"
     CDS             complement(2451..2636)
                     /locus_tag="ACHA52_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8940859.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2990..3235)
                     /locus_tag="ACHA52_002184"
     CDS             complement(2990..3235)
                     /locus_tag="ACHA52_002184"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940860.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3229..3630)
                     /locus_tag="ACHA52_002185"
     CDS             complement(3229..3630)
                     /locus_tag="ACHA52_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8940861.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="ACHA52_002186"
     CDS             complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="ACHA52_002186"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8940862.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5648..7414)
                     /locus_tag="ACHA52_002187"
     CDS             complement(5648..7414)
                     /locus_tag="ACHA52_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8940863.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7427..8221)
                     /locus_tag="ACHA52_002188"
     CDS             complement(7427..8221)
                     /locus_tag="ACHA52_002188"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8940864.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8231..9607)
                     /locus_tag="ACHA52_002189"
     CDS             complement(8231..9607)
                     /locus_tag="ACHA52_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8940865.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9727..10374)
                     /locus_tag="ACHA52_002190"
     CDS             complement(9727..10374)
                     /locus_tag="ACHA52_002190"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8940866.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="ACHA52_002191"
     CDS             complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="ACHA52_002191"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8940867.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="ACHA52_002192"
     CDS             complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="ACHA52_002192"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8940868.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13041..14672)
                     /locus_tag="ACHA52_002193"
     CDS             complement(13041..14672)
                     /locus_tag="ACHA52_002193"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8940869.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
BASE COUNT         4741 a         2933 c         1986 g         5139 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa
     1261 aaataggcta tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg
     1321 taatttcatt tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag
     1381 tagttttaat agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt
     1441 caattaaaaa ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg
     1501 caatttcagc tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat
     1561 caaaagaaaa aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt
     1621 ttaaatcttc aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg
     1681 tttcagcaaa ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc
     1741 tgccatttat taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac
     1801 ctgtaacaaa aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat
     1861 aaacactatt atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct
     1921 gagtttttat tttacctata gtatctacag gattttcttc aataatttca agtacaattg
     1981 cccttttaaa aactactctc aacttattga atatctgttt aatataattt ggtgataatg
     2041 gttgctcaat gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta
     2101 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
     2161 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
     2221 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
     2281 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
     2341 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
     2401 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
     2461 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
     2521 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
     2581 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
     2641 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
     2701 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
     2761 aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac
     2821 agcaagaaat catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat
     2881 ctatttaatt ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac
     2941 tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt
     3001 taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta
     3061 cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat
     3121 aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata
     3181 agataatgga tagtggaact aataggtact gccgcttttt agatttatct accataggtc
     3241 ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct
     3301 catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat
     3361 aatctttctt cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat
     3421 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata
     3481 cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt
     3541 tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg
     3601 tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac
     3661 taatacaaca atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata
     3721 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag
     3781 gctaatcgtt ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg
     3841 tattgatgac catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt
     3901 aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc
     3961 aaaattccac acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc
     4021 ttcgtaataa gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc
     4081 agctactgta tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat
     4141 ccatccatct agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac
     4201 ataccaacca taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac
     4261 atctggcgtt gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc
     4321 tttagttaat tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg
     4381 ttcatttgca atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc
     4441 cttgatcacc gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc
     4501 cacattatca tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc
     4561 atcaataact acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata
     4621 atgtgatgtt ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt
     4681 tacagcctca ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt
     4741 aaaataaaaa ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat
     4801 accaaattct tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat
     4861 actatataaa taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc
     4921 tttctttcct tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat
     4981 ttttccggtc cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct
     5041 tgtattaata gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct
     5101 atgaatacct gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc
     5161 attttcctgt actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt
     5221 gtttacagca actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc
     5281 aaatggtgta aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc
     5341 ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag
     5401 agtcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa
     5461 taaatcatta taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc
     5521 taaagcaagt tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct
     5581 taagtcaatt acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat
     5641 ctaaatatta aataaatgta tcctttaaac cacaataagc atctattgga cttactccct
     5701 taaattcaga aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat
     5761 cataagtatt aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta
     5821 cagatacaaa aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac
     5881 ttgctggcca gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca
     5941 agtcatattt tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat
     6001 ttttaatttt ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt
     6061 caacttcaaa tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat
     6121 ttccagcaac tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc
     6181 ttttatattt ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg
     6241 cttggtcagc cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag
     6301 cttcttgctt aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt
     6361 ttaaccctaa aattcttttt aaggcatcta ataaccgatc atcagtgata attcctttac
     6421 gatagccttc catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat
     6481 cacatccagc tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc
     6541 ctaacatatg actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta
     6601 agtcagtgat taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag
     6661 gattaaaatg ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc
     6721 cagcttctat taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta
     6781 aactattaat actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat
     6841 gtttagctgc aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt
     6901 ctaaaacttt atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat
     6961 ttctattaat atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa
     7021 tcccagaaac tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta
     7081 cttctacacc aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag
     7141 cagcaattaa tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct
     7201 catcagcagt tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac
     7261 tcaagtaatt tttatcacga ctagatccca tattaacaaa caattgacct attttttctt
     7321 ccaaagtcat agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat
     7381 atggtttttt tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa
     7441 gatttttcca aaaagtatct atatattttt gttcaatatc tacataatct cccgataaac
     7501 atttaaataa aacatcagtt cttaagtttt cccatgattc tttttcttta ttgaccaaaa
     7561 taggataaaa gaaaacttta tttttatttg cagcttctag atcaccagga gaatcaccaa
     7621 tcattaatat cttatccaaa tcacaaccat tttcaattac acttcgaata atatcttctt
     7681 tttttccttt atcttgacaa cacagttcat caactaatga taataattca tgtctttccc
     7741 attcttcttg aacagcttcc ttattagctg agcttacaat aattatcttc ccatattttt
     7801 tcagagcttt tatagtatct ctagcactta aaaagggttt atctaatcct tttgcttgac
     7861 taatctgttg attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag
     7921 aatattctat ttccctcttc aaagatggat tagacaattc ttttgttaga gatacccagt
     7981 gctttaatcg attaatcgat gttacatcat ttttttctaa tgttttaact aaacctaaaa
     8041 atctgtttat tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt
     8101 tttctctatg atgaacatca aaaatctctg cagcaattgg tccgaaaaag agattgtgtt
     8161 tataagtcat cgtatccata acacagccat ctgaatcaat acaaaatatc caattattca
     8221 tatttctctc ctatttatgt ttttgtttta aaagctcgca attttctaag actttatctt
     8281 tagataatgg ataaaggaaa aataaaatta atcctacaat cagaaaacaa attgatggaa
     8341 ttaatgttga agcgttatat aacttttcta aaacatctcc cgtttgtaca tttgctaaag
     8401 agtcatagcc tataatagaa agagtccaac ctccgacgcc tccagctaga gcttgaccta
     8461 tttttctagc aaaagaatat accgcatata cggtcccatc ttctctttga tcagacttta
     8521 cttcttgatc atcaataaca tcagtaatat ttgcccaaat aacagtatta aacacaccca
     8581 aacccatata gccaaccgaa ctaagaatta agaatacata catattacta gcttttatca
     8641 aaaataagat caaatatact attgctgaaa agaacattcc tactgatgcc agttcttttt
     8701 ttccatattt tttacttaaa aacaaagtta aaggcgaaac aactattaaa accaaaatag
     8761 gattgataaa attcattaaa actattccct gtgcatcatt atagtaatca ggaaatacat
     8821 aattattcat actcataatt aataattgag caaggagtaa gaaaatagcc gcaccaatta
     8881 tagaaagtaa tgccctattt ttaactaacc cttttaaaga ttttaacaat gaaactttgt
     8941 attgagattg gtcaattctt actcgttcag tagttagttt ataacatatt aaataacaaa
     9001 taagtgcaca tatcgaaaat attcccgcaa caattgtaaa agttgatcca ccacgaacta
     9061 tttgagcgcc tgaatcatct cttgtataaa taaataatgg tgttaataca ccaatgacca
     9121 aacttgctaa agtagctcca acatttcgga atgtcgatag cattgttctt tcttctgtaa
     9181 tagaagtaat agctgatgcc attgatccat aaggaatatt tatagatgtg taaaaaatac
     9241 tcccccataa tatatacgta acatacatgt atactacctt aacgctcata gaagctgaaa
     9301 tcatagaagt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc attcttaaaa
     9361 tccacggttt aaacttaccc tgcttagttg ctttcgatct atcaacaata cgccccatag
     9421 ttacatcagt aactgcatcg ataaatcttg ctactaaaaa taatgttcca acggcagctc
     9481 cgcttatccc caatactttt gtataaaaga tcattaaaaa agaactagca aaaatgaaag
     9541 taaaatcgtt tccaaaatct ccaaacatat atcctatttt gtctcttata ccaaattttc
     9601 tttccataca attctctccc ttctgttgtt aagcgttttc attctgatga aagtacgtac
     9661 tatactctga agcgtaaaga gtcagtattt attttttaca tttcagagta ttatttgttt
     9721 tttctcctaa taattcttta tctcataaaa cttatctata tatttactag ctactcgact
     9781 tacttcttca aaatcacctt tttcaacaga agccaataaa ttcccaccta caccaactgc
     9841 aacaactcct acttcaaacc aatcttctaa gttctctata cttactccgc cagtaggcat
     9901 tatatttacg tgtggtaatg gagcttgata ggcttttaca atggatggac caaaagcaga
     9961 acctgggaat agtttaatga tatctacgcc agcagtaagt gcctccttta tctcagtaat
    10021 tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctcag ctgtttgtcg
    10081 atcaaaagat ggactaacga tatattctgc cccagccata attgcgatac gagcagtaaa
    10141 cgcatctaaa accgtaccgg ctccgattat tacatctttt tgatcagcat attttttagc
    10201 aatttctttg attatttctt cagcattagg aactgtaaaa gttagttcaa tccctttaat
    10261 tccacccgca ataattgaat ttattgcttt aatggcctca tccttatttt tcccacgtac
    10321 aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat
    10381 ctccttcctt tatggataca tactttgtct agctgtacta acttacaaag aataaattta
    10441 agcttttatt tcaagtatta ttttctactt ccgatttcaa aaaaattaaa ataattatac
    10501 gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt
    10561 cggcctgctt taacccactc gcttagtaat tgacataata ctcgacgaaa atactcatgg
    10621 cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa
    10681 ctttgttgtg ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac
    10741 caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt
    10801 gctagtggaa gccaatcagt tggatttaat gaatataaaa ttgtttttgg taagcaccca
    10861 ctcttttcag cggtatcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc
    10921 atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga attatttctt
    10981 aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt
    11041 aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct
    11101 aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta
    11161 ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aatgatccac tcgttggctc
    11221 aatgcttgct tcaacccatt aaagtctttg attggtatag ccgctgtttg actaagtttt
    11281 aaaatatagt tcgaaaagcc tgatgatttg atactccaga ccttatctgg acggaaagta
    11341 ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatacca caagtcatct
    11401 gtaggatcat ccgttgtaca gatggctttc acatccatca tctccaacaa ttctttcggg
    11461 cgataggaat ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc
    11521 tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa
    11581 ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca
    11641 tcacctggac cactgatcaa ctcttcttct ataccattag ctcgcatcaa tcgccattta
    11701 taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta
    11761 ttttctagca aatatgctgg gtctaaatga caatgatagt cgataattgg ttggtgtttt
    11821 gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa
    11881 aacatattta aacactcctt tccttccgtt agtttttaga agagaaaatt tttcctctaa
    11941 ataaactccc ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt
    12001 taaataaact gcacctagag ctcggtcata taatccataa ccaggtctac cggtttctcc
    12061 ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt
    12121 ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg cttcgtaaaa
    12181 atcgagtcca ttgtcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc
    12241 ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact
    12301 tccagtacat aatgtaatac cattagaagg agaatccact acattacaca accaatctaa
    12361 atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg
    12421 gtgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt tgagaaaata
    12481 tgcaaagttc tctcgtaatt tctcttcatc aacttcttta taggcttcta ataattgctt
    12541 aatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc
    12601 tacttctcta ataatttcct ccggatcagt agggatattt tttgaaatat aggctaacgt
    12661 agaagaccca tcatctaaac gataatccaa atccgatttt acccaatcaa atactggcat
    12721 aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata
    12781 attctcaatg tattgatctc gtgttggtaa accaattttg atatcatcat gaacattaac
    12841 actctcaata actttcaagg taagtccatt ggcttcgact tcatctttta aagatttaat
    12901 tcgttctctc ggccaaacct ctccggctgg tatatccatt aatgttccaa caattccttt
    12961 cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca
    13021 tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt
    13081 ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcagt tttctttcct
    13141 accccaactt gatataaatc taaaccaaag atttctttat tttctaaaat ttcctgtacc
    13201 gcacaatgaa tctttgaagg atcttgttca cctaatttca atactgataa tttatcctgc
    13261 aactctacca ataacggatc aggacttggc acaaaatttt ccccttgatc atcgactgcc
    13321 agtaaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt
    13381 ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa
    13441 gtatctgttg caattcgttg tggtgtatct ggaatatagg gattaggcaa acgtttatta
    13501 attacttcat caataaattg ttttggatca ataatgcctg gattttcaac tactggtaaa
    13561 ccttctaaat aacctattcc tttgattaaa ccaaccaaat catcatcctg catctctgct
    13621 gaaattgatt gatatcctag cagacaacct gttactgcca aagcagtatg aagtggattc
    13681 aagcttgtcg taaccttcat gatgtctgct ttatcaaccg tctctctatc tgtcataatc
    13741 acaccagcct tttccaattt aggccggcca tttggaaaac tatcttcaat aactaaataa
    13801 tgaactacct cagtattagt gaaagcagca atattggtat gtttttttgt gtgaatgata
    13861 tctaaatcct cgagccccat catcaataga tcttccttta cattctctga aggatttggc
    13921 gtaattcgat caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca
    13981 ataaattttt cagaaacata tccttctttt tgccaagctt ctgcaatcgt aaaaatacta
    14041 tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac
    14101 tctccagcat taaatctgct atataacaag gatgttacaa tactcatcgt atgttgagga
    14161 ttgctaggtc cagatttgat atcagctttg actaccgaaa agaattgccc tttagaatct
    14221 tttaaactgt aacctttttc agtaatagta aacgttgtaa actgtaattt aggatcttca
    14281 aaatatttaa ccactctttg atagtccgct atattcttcg gattgcaata taaactatca
    14341 gccgttactg ccaaaagtct ttttttgagt tcaccatctc cactcataac aatttccaaa
    14401 atgtcattgt tatagtcatg ataagctttt tccactactt cttcatcaaa tgtttcacac
    14461 acaactactc ctgtttctaa ttcattttga tctgctaagc attgagcaat ttctgcatga
    14521 aatcctcgat aaagatttcc tccaccaaaa tgaagccata ctggatgttg ctggccttct
    14581 tgcttaactt ctttgacatc aaaacacggc agtagaatct gcatttcatt gaattcttcg
    14641 atagaatttt gccaattttt aatcgaaacc ataattaacc tcctcaaact gatatgttag
    14701 ttttgataca ttttatattt taaaacccat tgtaaccgtt gtcaatataa aaagggaata
    14761 aacttgcttt tttctaccat atcagaatga ttgtatttt
//
LOCUS       DAYYGY010000071        14489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000071 DAYYGY010000000
VERSION     DAYYGY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.072"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..147)
                     /gene="tnpB"
                     /locus_tag="ACHA52_002194"
     CDS             complement(<1..147)
                     /gene="tnpB"
                     /locus_tag="ACHA52_002194"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8940870.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCG"
     gene            complement(137..331)
                     /locus_tag="ACHA52_002195"
     CDS             complement(137..331)
                     /locus_tag="ACHA52_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940871.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(399..1001)
                     /locus_tag="ACHA52_002196"
     CDS             complement(399..1001)
                     /locus_tag="ACHA52_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940872.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMVSAP"
     gene            complement(1149..1691)
                     /locus_tag="ACHA52_002197"
     CDS             complement(1149..1691)
                     /locus_tag="ACHA52_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8940873.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1826..2215
                     /locus_tag="ACHA52_002198"
     CDS             1826..2215
                     /locus_tag="ACHA52_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940874.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            2219..2983
                     /locus_tag="ACHA52_002199"
     CDS             2219..2983
                     /locus_tag="ACHA52_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8940875.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            3112..4059
                     /locus_tag="ACHA52_002200"
     CDS             3112..4059
                     /locus_tag="ACHA52_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8940876.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            4189..4929
                     /locus_tag="ACHA52_002201"
     CDS             4189..4929
                     /locus_tag="ACHA52_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940877.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(5220..5888)
                     /locus_tag="ACHA52_002202"
     CDS             complement(5220..5888)
                     /locus_tag="ACHA52_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8940878.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            6022..6309
                     /locus_tag="ACHA52_002203"
     CDS             6022..6309
                     /locus_tag="ACHA52_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940879.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6725..8263
                     /locus_tag="ACHA52_002204"
     CDS             6725..8263
                     /locus_tag="ACHA52_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8940880.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            8260..9225
                     /locus_tag="ACHA52_002205"
     CDS             8260..9225
                     /locus_tag="ACHA52_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940881.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            9212..10534
                     /locus_tag="ACHA52_002206"
     CDS             9212..10534
                     /locus_tag="ACHA52_002206"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8940882.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10597..10701
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10775..11518
                     /locus_tag="ACHA52_002207"
     CDS             10775..11518
                     /locus_tag="ACHA52_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940883.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11533..12525
                     /locus_tag="ACHA52_002208"
     CDS             11533..12525
                     /locus_tag="ACHA52_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8940884.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12537..13274
                     /locus_tag="ACHA52_002209"
     CDS             12537..13274
                     /locus_tag="ACHA52_002209"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940885.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            13264..13986
                     /locus_tag="ACHA52_002210"
     CDS             13264..13986
                     /locus_tag="ACHA52_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8940886.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(14070..>14489)
                     /locus_tag="ACHA52_002211"
     CDS             complement(14070..>14489)
                     /locus_tag="ACHA52_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8940887.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4837 a         2470 c         2982 g         4200 t
ORIGIN      
        1 cccgcaaaat aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag
       61 actggcaaga ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg
      121 tttggtttga agtaaatcat aaagcattgg acaacagttc tttcaaaata gcttgaagaa
      181 tataattgtt aataccgtta tagatcaaaa cttcagaacc attctctctt ttgatgcgca
      241 tcgctaactt ggcagccgcg ggtttacctt tttctggtgc actcttttcg tttcctaatt
      301 ggacaggaac aatctcattt aatttataca tagaaaacct ccagacttct tgatagctca
      361 agtatatctt gaggtgcatt tgttagtaag gtactgttct atggggcgct taccataaat
      421 acacctagac tgacgatgaa gagcatatcc aacagatttc ctgcgacttg gatcgtcgaa
      481 gggttggtca gtggcattaa agcaaggaca aaaaaggaaa acaagctgta aataaaacag
      541 gtgacaggaa ctaatcgaaa gaagagcgga catttcaatt ggctaaaaat gagtgaaaaa
      601 ataaaataag taaaaatgga taaaatcacg actaaaccat tcatgataaa gaagaaaagc
      661 gtattgtgat taaacgaata aaaaagaatt gctaaaaaag aaaacgtttt aaatgcaatc
      721 aatacgcttg aagcttcgtt taacgtacca ggatgtcgat gaaaaaaata ggcaccgaaa
      781 taaacagaaa acagtaagcc gcaaagagaa gacaaaatca aaaaaaacgg attttcttcg
      841 gcttctgtga tatcagaatg atccaaaagc aaccagccta atgcactaga aggcgcatgt
      901 gaggtcttaa ggtatgaata acatagaaaa aataagatcg tcaaagttgc aaaaaaagag
      961 cctaaatgta aaaaattagt ggttctacgt tgaatttcca taatatctcc aaattaaatt
     1021 gataagtata taaagtataa taacaaattg atgagtatat gtcgatgttt tttaatcaaa
     1081 ataataaata aaaactgtct aacatttaaa atttaattta gatattagac agtttttaag
     1141 tttgtgagtt attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata
     1201 tattttggct cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac
     1261 agttcacgtt cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc
     1321 cccattgcag aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt
     1381 ttatcttctg cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc taatgccaat
     1441 ttgtcttcaa tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat
     1501 aaattcagtg gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg
     1561 atctcgacca tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa
     1621 cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt
     1681 ggatcaatca tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta
     1741 agtgaagaaa aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat
     1801 tataaagata ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca
     1861 cgtgctaagt ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga
     1921 ggtagagaac attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt
     1981 ctttttgata ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc
     2041 tatttatcca ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt
     2101 tcccgagcca ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac
     2161 tgttggagca tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt
     2221 gaatccaatt atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa
     2281 agaattaaga gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa
     2341 ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc
     2401 agatgcgatg aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt
     2461 ttgggatagt atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa
     2521 aagaggtctt ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac
     2581 atgcgccttg ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc
     2641 tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt
     2701 accaaaagac tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc
     2761 ggaaaagtta gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc
     2821 ttatatacaa acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat
     2881 tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa ataccactaa tggaagaagc
     2941 ttccactttt ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac
     3001 tcatgacgat agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa
     3061 atttggtata attgatagta agaatgacaa catgttagaa aggaagagag catgcccact
     3121 ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat
     3181 catccggaac aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg
     3241 gattaccgtc caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa
     3301 ttatttatac tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga
     3361 atcgctaaac ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc
     3421 gacgtcggtt cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg
     3481 atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat
     3541 gtagacagcg ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat
     3601 tatcaaacga ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa
     3661 actggttatt tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca
     3721 aatcattcga cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa
     3781 acttgttttg tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat
     3841 gctggaggac acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac
     3901 caaatcagtt cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta
     3961 tgtggagaaa tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt
     4021 ttttttcctg aactagttat cagagagaca acgaattaag tcttattact atatatatat
     4081 ttatgaaatt catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa
     4141 tctgtgctat tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc
     4201 agacgaaaac atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac
     4261 gacaagaagc gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc
     4321 ccgtttgaac agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc
     4381 aagcgtcgtg caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat
     4441 aaaggaaaat taccggatta taccggccat taccaatcag aaatcacatc gacaggacga
     4501 aaaaaacttt ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc
     4561 aagcatacta cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt
     4621 cctaaatatg tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat
     4681 catttaatcg aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg
     4741 acgccgtatc aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa
     4801 acgttagagg aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta
     4861 gaacgctcat tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat
     4921 tttaaataag tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcggcg
     4981 tttccttatt ttatactcta tccgaaagtt tatatcgtat cctcaaattt ttttgagttt
     5041 aagcgaattt tttctcccaa taagtaagaa aataatggca aaataaaaaa acaaactatt
     5101 ttcgcagatc gtttgcaaaa atagtttatt tttttataaa gaagatttta ctgaataata
     5161 tcattttagt ttacataatg atatttctgt aaattaaata aagtagtgaa aaataagaat
     5221 cagtgctcta actgttcaag aggatcaagt ggttcaaatg tttctgtagt ctgttttgct
     5281 tccaattggg gcaaaacaga tccaatttcc agatacttca ctttattcaa tgtataagat
     5341 agaatagaac agctaagtaa tgtatacaga aaatgtgtga gatccaaata aagcaaggag
     5401 acaactaaaa taccaaaagt aatcaagaaa agacttttgc caatcttaat atgaaaatat
     5461 ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc ttccgccgaa tcgaaagacg
     5521 agaccgcaac caatgccaga aagaatacct gtcatcaaac tattgatcag tagactatct
     5581 acagcaaaat gtatatgaag gctctcccat aaatgtaaaa agaaaactag cgcaaatgtt
     5641 ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa acaatgagat aagcaataaa
     5701 ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc caaaaagatt atgcaaaagc
     5761 aaagcaatac cagtaacccc accatctgcc agcttagatg gaatattgat tcctgtcaca
     5821 gctaatccgt agattcctgc gcctaaaatg attccgcaca attctgtaaa taaatttaat
     5881 tttttcattt ctagcctctt ttcttttgaa aattttcagc aacttcttta ttatactgac
     5941 ttatactctt acttcaagag gaaaatgaaa aaaaattatt tttggctgtt tctttttatt
     6001 tatcaaataa ggagtggaaa aatgcgagca gcagacttat tgacaattct tacaaaatca
     6061 cccaaaaaaa cagagtatca aggagttttt ttggctcaag aacaaaaact ggtgcctgtg
     6121 acaatggtcc agataaataa agcaaatcaa tttatctttt tatttgaaca cggcaaagca
     6181 ccacttgcta tgaaagacat ctttcttttt ttgatgaaga accgtgagaa agctgtttat
     6241 tatcagtcaa aagaagaact cattcctctt tatggagtga aagagataga aaataaagtg
     6301 gtgatataag taaaaataag aaaacaagac gtttatgatg tcaaaacaac cattttttac
     6361 atttttggca aaaaagtgtc atgatacagt ataatattcg ttgaacaggc caataaaggt
     6421 gttctccccc acgagatttc tactttattg gctgtttctt tttattaaaa taaaagttgt
     6481 gtgtgtaaaa aagatatagc tgattccaag tgacaatttc gtatagattt ttatggtatt
     6541 cgtagtgaaa aaacgagaac caaaaagttg atccgacttt gttgcttgga ttcttttttt
     6601 gtcagcatat ttagaggtat ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag
     6661 acaagttagt tgggaaaaag gaaagaaatg tttatactta aagaataaca gaaaggaagg
     6721 tcgcatgaat aattttatac aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc
     6781 atttcaacat ctgtccattt ctctcatttc tttgctgatt gcagcactga ttgctattcc
     6841 tttagcgatt tgggcagcca atcataaaaa aatggcagaa gtcttattgc agatcacggg
     6901 tgtgctccaa acgattcctt cacttgcctt attaggttta cttatcccat ttgtagggat
     6961 cggaactgtt ccagcattga ttgcacttgt tatttacgct ttgcttccga ttttccaaaa
     7021 cacgtatatt ggtctagctg aaattgaccc gtctattgaa gaagcagcgg tagcatttgg
     7081 tatgtccaga atgagacgct tgctgaaagt agagcttccc atcgcattac ctgtcattat
     7141 ctcaggtatc cgaactgcct tagttttaat catcggtacg gctactttgg cagcattgat
     7201 cggagcaggt ggattaggga catttatttt gttaggtatt gaccgaaata cacctgtttt
     7261 gacattgatt ggtgcgatca gttctgcctt gcttgcaatt atattcagcg gactgattcg
     7321 ctggttgcag cataagaaac ctcgatattc catcattact ttagcagcag tggtgtgttt
     7381 atttggcggc gtttctttat atcagtctgc cttgtttaaa aatgaaacca tcacgattgc
     7441 tggaaaatta ggagcggaac ctgaaatcct gatccaaatg tataaagaat tgattgaaga
     7501 caacacaaaa acagaagttg aattaaaacc gaattttggc aagacgagtt ttttgttcaa
     7561 tgcactggat caaggtcaaa tcgatatcta tcctgagttc acaggtactg tcctagaaag
     7621 cttggtgaag gtacctgaag caatgaaaag tgctgatttg acaaaagaaa aaacatatga
     7681 cgaagcagaa gaattattga ataagcaatt cgatatgaca ttattaaaac caatggctta
     7741 ccaaaatacg tatgccttgg cagtcaagag atcttttgcg aacgaacatc aacttaaaaa
     7801 gatttccgat ttggcagcta tctctgatca gatcaaagcg ggattcacac tggaatttat
     7861 tgatcgttca gatggatata aaggaatcca agaagcatat ggcttgaatt tttcaagtgt
     7921 acaaagcatg gagccaagtt tgcgttacca agcaatcaat aatggagatg tcaatattgt
     7981 ggatgcctat tccacagata gccagctcaa ggagtacgat ctagtgactc tagaagatga
     8041 tcgagggtta ttcccagctt atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga
     8101 acatcctgaa atcgttaaag cactgaataa attaagcgga aaaataacag aggatcaaat
     8161 gattgatatg aattaccaag tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat
     8221 cttcctgaaa aatgaaggat tagtaaagga ggaagatcag tgactaaatt aatcgagttc
     8281 aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg atgacctaaa tctggcaatc
     8341 aaaaaaggag aaatctttgt tttggttggt ccttccggta gtggaaaaac aactactttg
     8401 aaaatgatca atggattatc taaaccatca gcaggagaca tctacttcaa aggaaaatcg
     8461 ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc
     8521 gctttgttcc cgaccatgac agtcaagcaa aatattgaag tcatccctga aatgttaggt
     8581 tgggaaaagc agaaaagagc agaccgtgta gatgaacttc ttcaaaaagt tggcttatct
     8641 cctgatattt atcgagatcg tatgcctaga gagttgtctg gaggagaaca gcaacggatc
     8701 gggatcatcc gagctattgc tgcaagccca gatgtgattt tgatggacga gccatttagc
     8761 gcattagatc cgatttcaag aaactcatta caagaattgg ttttgtcctt acacgaagaa
     8821 ttgggcacga ccatcgtttt cgttacgcac aatatggaag aagcaatcaa gctaggtgat
     8881 cgtattgcat ttatgaagga tggagaaatt atccaatgtg acacaccaga gcagttattg
     8941 atgaatccta aaaacgacta cgttcggcat ttctttgatg aaccgaaaca gacgaaagag
     9001 tggcgagtag aagatttagt cgtcaatgga tatttcttaa atgaaatacc agaaaatgct
     9061 cgtcaacaag tttgctttga tacgcctatg aaagaagtat attcgcttct ttcggtgtat
     9121 ccaagcattg tgatagtaga aaagcaaaga agtattgggt cgctgacaag taaagaaatc
     9181 tttgcttttt taagtcgaga ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg
     9241 ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa gaagcaggta aaacagtact
     9301 agtggtcgaa gaagatctat ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa
     9361 agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt
     9421 taacgagatc ccaactgcca actgggaaga acttcaaaaa ttcaaacgaa catttacgga
     9481 tccagtccca gaaagccgaa aaaaacaatt agcagaagct gaaatcgatc atctttcagg
     9541 aaccgctcgt tttttggatg attcatctat tgaagtcaac gaagaagtct tccatgcgga
     9601 ttacttagtt ttggcaaccg ggcaaagacc tacgatctta ccagtggagg gaaaggaata
     9661 cttgaaaact agtgcagact ttttatcatt gcctgtactg cctaaagaaa tcatttttat
     9721 cggtggtggt tatattgctt ttgagctagc tacgattgcc aatgctgcag ggagcaaagt
     9781 aaccattgtc catcataatc aacggccatt aaaagaattt gaggcgtctt tggtagagga
     9841 agccgtccat caaatggaag catctggcat tcagtttgct tttggtgttg aaacccaaaa
     9901 gatcatttca gaaggtactc gctatcgttt agtcggaaaa gagacggaat tagtagcaga
     9961 tatgattttt tgtgcgactg ggagacagcc gaatacagaa tctttagcac ttgagcaagc
    10021 gaatatcgtc tttgataaac acggaattgc agtcaatgat tatctgcaaa cgagcaatcc
    10081 aaaaattttt gcgtgtggag atattgtttc aaggaaaacc ccaaaattga caccagtcgc
    10141 cacttttgaa ggaaattatg tagctaagcg gataacggat gcaacaagcg aacctatcaa
    10201 atatccgatt atacctacga tcgtctacgc cagtcctaaa cttgctgaag taggagttac
    10261 aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg gatcttacca gttggttcac
    10321 ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg acctttgacc agcaaaatta
    10381 tttgattggt gctgcagtga ttagcgagca agcagacgaa ttgatcgatg atttgacact
    10441 cgtcatcaat caaaaattga ctaaaaaaga gttggatagc tatatcatgg ggtatccgac
    10501 attggcaagt gatctctcct atttattgaa ataatcagca tgataactaa agcgcttgtt
    10561 gctttttcct tcttgtgcta cactttaaaa aatgaatacc tgtttctggg gatgtcgtag
    10621 acgacatgag agtaagttgt gaacttagac ccattgaacc tgtcagttaa tactggtgaa
    10681 gggagaaaca tagcagacgc ttgaagcggg ttactatgtc gtgcccattt caagcgtttt
    10741 ttgttattca tattagaaga aaggaggtaa caaaatgaag aaaaatcttt atccagcagt
    10801 cagtatggca ttcattttaa ttatttggca gattgttgtc atagtttgga aaatacctgc
    10861 ctttatttta cccggacctg ctgctgtatt ttctgcatta gcggcagatg cacaagtatt
    10921 actttcccat tctgtcacga cattaaatga agcagtgctt ggtttggtaa tagctagcgt
    10981 tctttctttt attactgcct tggcaatgga ttattggaag tttttagaaa tgtcttttta
    11041 tccgttacta gtgatttcac aaatgttgcc aatcatggtg ttagggccat tacttaccct
    11101 atggtttggt ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa gctattttcc
    11161 gatcgtagtt tctttttcgg atacattgag gaaagtatca aaagagcaaa tcatcttttt
    11221 gaaaacgatg ggggcagata cagggaagat ttatcgaata tataaaatac cagcgggttt
    11281 atccggcttc ttttccggtt taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt
    11341 tggagaatgg ctaagcgctc aagcaggctt agggtattac atgatacgcg taaaaaacag
    11401 ctaccagatc gataaagttt ttgctgcgat catttgtgtc attctattga gtttattgct
    11461 gaatggcgct tgttcattat tgaaaaaagg gtattatcgt atgctttata ttttttaaga
    11521 gaaggggatc aaatgaaaaa attatggctt ttattaccgc tactgttgct tttatccgct
    11581 tgtggcactg caaaagaaac aagtagcaaa caagaaataa aggatttgaa aaaagtgaca
    11641 cttgttttgg attatgtacc gaatactaat catactggca tctacctagc aaaagaaaaa
    11701 ggctattata aagaggctgg tttaaatgtc caaatcatcg aaccaggaga taattcgaca
    11761 agtattggtc tagtaggtgc ggacaaagca caatttggtg taagttatca agaagatgtc
    11821 acttatgctc acgcagatgg acagaatata ccagtcaaag caattgctac agtgatcaaa
    11881 cataatactt ctggattcgc caccctatca gacagtaaca tccattcacc aaaagatttt
    11941 gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg
    12001 atggaaaaag acggcgggga cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg
    12061 ccagaaagct taggaaaatc ctcagatatc caatggtact ttgaaggttg ggatatgatc
    12121 aaagcaaaag aagcgggtat tgaactaaac tatatcccat tgaaagaatt agatgaacgg
    12181 ttagattact atactccggt aattattacg aatgatcaac tgatcaaaag tgatccagaa
    12241 ttggtacagt cttttatgga tgccacgaaa aaaggctatc aggaagctat taaggatcca
    12301 aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa
    12361 gaatcacaag cattcttgtc aaaaaattat acggatgatc ctgagaattg gggattgatg
    12421 gaagaaaaag tttggcgcaa ttatacagct tttatgcagg aaaacggatt gatcaagcaa
    12481 gatgtacctt cgcaagaact attcactaat cagtttataa aataggagtg atagatatgt
    12541 caatccaaat cagccacttg aatgtagtca ttgaaaacct gaaaatatta gaagacatcc
    12601 atttcactat ccctaaagat acctttacat cgattgtcgc gccaagcgga gccggtaagt
    12661 caacattgct gaaaacttta acaggagtcc agcctatgac ttctggaacg atcaaagtag
    12721 acgatcaaaa agtaactggt ctccatacag catttagtta tatgccgcaa gaagatatgt
    12781 tacttccttg gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa
    12841 caatcaatga agaacaagta agggattatc tggatatctt tgggttaaac ggctatgaac
    12901 attttttgcc ggaacaatta tctggcggga tgaagcagcg aacggccttg ttacgaacaa
    12961 tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt tggagcgtta gatgctatga
    13021 ccagagggca gatgcaagac tggatgttga agctgcctaa aaaagcaaag cggactactt
    13081 tacttgtgac gcatgatatc gaagaagcaa tttatttatc agacaggatt cttgtgcttt
    13141 ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg
    13201 aatggctact gcagcaatca gagttgaagc aaacgattta tcagttattg gcaggtgaat
    13261 tagatgttaa gtgatgctca ttgtcattta gatgggagtc agagtttagc actccttcag
    13321 caggaacaca cgattttgac gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac
    13381 agacagcttg cagcaagtaa gactcaagcc ttaagttacg gtatccatcc ttggaaagca
    13441 gatagctata cgtttgaaca agttgagcct tttttgaaaa aagcaaggat catcggtgaa
    13501 atagggttag ataatatttg gacgaatgta ccgatgacta cacaaaaaaa agtatttgag
    13561 cgacagcttg cttttgcagc cataaatgag aaacctgttg tactgcacac aaaaggatgc
    13621 gaaaaagaga tcttaaatta tatccaagct taccctaatc actatttgat ccattggtat
    13681 tcttctttgg actatcaaca agactatatt gatttaggct gttatttcag cattggtctt
    13741 gatttgaaaa agaatccagc agtctggcaa ctagctaaag cagtcccaat cgaccactta
    13801 cttattgaaa cggatggact acatgcagca gagtgggctt taggggggat tttttcagaa
    13861 aaagattatg tctcactgct tgaagaacag ttaaagacaa tcgctgagat caaagagcta
    13921 tcagttgaag aagttaaaaa aaatacttat caaaacttgg aacgtttcat tagattaggt
    13981 aaataaaaaa gagatgtgac acaactagtg tcgcacctct ttttggcttt ctttgtataa
    14041 aagtaaactg tttgattaat aaatgaatct tagtaccaac cgtttgcttc ccagaatgct
    14101 ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg
    14161 tttgcagcag agtagtcacc atttaagtaa gaagaatcta attggtaacg tccgatgtaa
    14221 cgtccgttcg ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca
    14281 gagcttgtgc ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca
    14341 gcaacaggcg ctgcttgttt ttccgtttga accgtttctt gtacaacagg ttgttcagct
    14401 tggactggtg cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc agagccttct
    14461 gttgggattg ttaattgttg tcctgagta
//
LOCUS       DAYYGY010000072        14326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000072 DAYYGY010000000
VERSION     DAYYGY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.073"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..1253
                     /locus_tag="ACHA52_002212"
     CDS             102..1253
                     /locus_tag="ACHA52_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8940888.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1362..3401)
                     /locus_tag="ACHA52_002213"
     CDS             complement(1362..3401)
                     /locus_tag="ACHA52_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8940889.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3459..4016)
                     /locus_tag="ACHA52_002214"
                     /pseudo
     CDS             complement(3459..4016)
                     /locus_tag="ACHA52_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(4164..4330)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(4555..4761)
                     /locus_tag="ACHA52_002215"
     CDS             complement(4555..4761)
                     /locus_tag="ACHA52_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940890.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(4777..5391)
                     /locus_tag="ACHA52_002216"
     CDS             complement(4777..5391)
                     /locus_tag="ACHA52_002216"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8940891.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(5538..6221)
                     /locus_tag="ACHA52_002217"
     CDS             complement(5538..6221)
                     /locus_tag="ACHA52_002217"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8940892.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(6330..6788)
                     /locus_tag="ACHA52_002218"
     CDS             complement(6330..6788)
                     /locus_tag="ACHA52_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8940893.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(6872..8635)
                     /locus_tag="ACHA52_002219"
     CDS             complement(6872..8635)
                     /locus_tag="ACHA52_002219"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8940894.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(8856..10316)
                     /locus_tag="ACHA52_002220"
                     /pseudo
     CDS             complement(8856..10316)
                     /locus_tag="ACHA52_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10524..11450)
                     /gene="glsA"
                     /locus_tag="ACHA52_002221"
     CDS             complement(10524..11450)
                     /gene="glsA"
                     /locus_tag="ACHA52_002221"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8940895.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(11473..12900)
                     /locus_tag="ACHA52_002222"
     CDS             complement(11473..12900)
                     /locus_tag="ACHA52_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8940896.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(12931..14145)
                     /locus_tag="ACHA52_002223"
     CDS             complement(12931..14145)
                     /locus_tag="ACHA52_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8940897.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4078 a         2913 c         2454 g         4881 t
ORIGIN      
        1 cttttggatt gttggttttt ttgtgagtat attttcctat tttttcaata atgtatgtta
       61 tgctaagttc gggctatatt ttatacaaag gaatgatgag aatggaattt attggtattt
      121 tatgcctgat attgatttca acaacgattg gctcacatat ctcgcggaga ttaggtatcc
      181 cagcagtgat tggacaatta cttgttggtg ttttgctagg ccaagctgga ttgaattggg
      241 tcaaacctga tattctagtc catgactttt cggagattgg tgtgatctta ttaatgtttt
      301 tagctggtct ggaaagtgat ttatctcttt tgaaaaaata tttccgacct ggtatgttcg
      361 ttgctttgct tgggatcatc ttcccggttg tttttggctg gttgacaggc aaagcattcc
      421 aagtttcggg aaatgaagcg attttctttg ggattattct tgcggcaact tctgtcagta
      481 tttcagtaga agtattaaaa gaactaaacg tagtgaatac aaaagaagga tcaacaattt
      541 tgggtgcttc tgtggtagat gacattttag tcgttttagt tctaagtttc agcctatctt
      601 ttctaacggg ggaagcttct agcaatgctt ctttaccgct tgttttgatt gaacagctaa
      661 tttattttgt ttttattttc ttattagtca aatggattgc gcctttccta ctttcatttg
      721 cagaaaaagt gtacgcaaat tcttctatta tcatcgtatc gctagttatt tgtttaggaa
      781 tgtcttacgt agcggatctt gttgggctta gctctgtcat tggtgctttt tttgctggga
      841 tagctgtcag tcagacaaag gttaaagaag aagtatacca caatgtagag gctttaggat
      901 atgcagtatt tataccagta ttttttgtca gtgtaggatt agaagttgat ttttctgact
      961 ttagtgaaca actactcttt atttctatat tgacgcttgt agcgattttg accaaattgc
     1021 ttggaggtta ttttggtgct cagattgccg gtttttctca acatagttca ttgatggtcg
     1081 gtgctggtat gatttcgcgt ggagaaatgg ctttgatcat tttacaaatt ggacagcaaa
     1141 gtcaattgat cgaaccacag tactattcac ctttagttat cgtgatttta ttaagtacac
     1201 ttatttctcc acttgttctc aaatatttta caaaaaaagt ttatgaagtg tgagtaaatc
     1261 aaaaagaagt tgtgaaagag acatcgtgaa aaccgtttat ttgaaaaaag gactgtgacg
     1321 ataacgccac agtcctttta ctgtacttaa aaagcaattt tttattgata attttggttg
     1381 aggtattgca atagttccga tgctcgtttg gttgctgaat catcatcttc tttattttcc
     1441 aacatgctga ccatcatata accttggtta tcaagattga aagcaaacaa gaaactgttc
     1501 tcttgcccct ttacatcctg tttttctttg atttccgctg taccagtttt cgctgccaac
     1561 ggaatcccta aagcagaaag agaatgtgct gtaccattta catcttgcac aacttctctc
     1621 agatctggca cgatcgtttg tactgctgtt tcgctgatga cattcttctt atcttttgtc
     1681 tctttatctg caatcaattt aggatagaca agtgtgccat tgttggtaaa aacagaatac
     1741 attgctgctt gctggatagg agtaattaga agttcgccct gtccatatcc agtatcagct
     1801 agcaagatat ctgagttaaa gctatcttca ttagaaattt gtgctggatt catactaatt
     1861 ggcaaatcaa ggtcttcacc aaaaatgaat ttatctaagc ctgtacgaaa ttttttctca
     1921 cccattttca acgtttcttg tgccgcatat atattatcgg aatagatcaa agcagttttt
     1981 aagtctactt gtgatacatc actactgaca cgcgttgttt taaaagatcc ccaagaactg
     2041 tctttttgcc atttaagccc gttgatcgtc aacacttcat ttggatcgat agtgccgttg
     2101 tcgagaccga ttgctgctgt aatcatttta aacgtagatc caggagcata acctgtcgca
     2161 aatcggctga tgaatggatg ttcaggattt tcttcataag ctttgtaatc ttcttgtgag
     2221 atcccgtttg tcattttgtt tggatcatag cttggagagc tagcaagcgc aagaagatca
     2281 ccggtttttg gcgttgtcgc aacagttgat gcagcttttc ctcctagact gtcaaaagct
     2341 attttttgtg ccttcgcatc gattgtcaat ttgatatctt tgccattttg aacttcatgt
     2401 tcgatcagaa cttttttctc tacaccgtct gcatctgtaa tgcttaattt cccacctgtt
     2461 gtcccacgaa gatccttatc aaaagccatc tccaaacctg agcgaccgat ttttccatta
     2521 ctgcttaatt ctggattctt atcaatatct tctgctgtga tatctcccac gtaaccaatc
     2581 agctgagcag ctgcttcacc caaaggataa tatctgccgt ctacttcttg aatggtagct
     2641 ccagctggaa gttctggcgt tgctccatca atgattttca atgggacaaa gtaatcgggt
     2701 tgtacccagc tttgtgaaat cgcctgattg atagcatctt ctgttaagtc gaaggaggaa
     2761 gcaatcgctt tgatattggc tgttttttcg cctccatctc caagtttact tggaacgact
     2821 cctaattgtt ttagtttgcc tgttgttgct aatggttcac catttcgatc tataatattc
     2881 cctcttgctg cttcttgtgt ggtcaggctt actttgtcat ttccttccat ttctggaaaa
     2941 accaagttgg gttgccagtt gatcgtggtt tgaccatcat ttctgtctaa tgttccttta
     3001 taagaaagat ctttcaattc acctaatgag gtattcatct ttgctttata tgaaaaactg
     3061 taagtagaat cgtctttttt atctactttt agattcgata tctgaagtcc tttgacatca
     3121 gcagcaccgt atatattctg gtatttatct aagatctctt tttcagataa tgcactttta
     3181 tttgccgcct tttttgatgt catttctgca gctttgttat aatctccctt gttcaaagct
     3241 tggacaaatt gctcaactgt cttttctcca gcttctactg cttgggtttc ttgatagtgc
     3301 tggtagtaaa aataaccgcc acccgcagct gctattaaga tcactgcgcc ggcaatataa
     3361 gcgcctgttc gatttttgcc gtgcttgtca cttcttttca ttttttcctc cttctcttgc
     3421 ttgtcattcc tttatttcac tatttatacc tgtttattct aacatatgtc tttgaggaaa
     3481 tccataccac aatccagaag cacactttct ggaaggttcg tttggatact accaagtaat
     3541 ttcccaaccg ctttgggtag cttgaatatt gaagttacgt ctttcagctg ctgagagata
     3601 gctttgatca cttgctgctg ccttctgatc ctcccgaagt ctccttcttt gtcttcccga
     3661 aatcgagcat actggagtaa agtattacca tccatcactt gctctccttt gtttatatat
     3721 acgccatcta aatctagaga tttttctgca tcgatcttga ctccaccagg aaacagttca
     3781 ttaacgcaat cgataaaatc ttgaaatgtg atacttgcat aatagggagc ttcaaatttg
     3841 aaattttcct ttaatgtttg atttaataaa tcaactcccc cataagagta agctgcattt
     3901 atcttattca ttccgtaacc tggaatatcg acatatgaat ctctcatgat agagatcagt
     3961 tttgcttgtt gtgttttctg gttgtattgt gcaaccagaa gtgaatccga gcgacctcca
     4021 ccaatcgatc ttttttcacc gcatctcctc ctcacacagc aatccacttg tcaggtgagg
     4081 agcgagtttg tctcatcaaa taagtcagtc acaaaaatat aaagaaaagg tcagaaacct
     4141 tttcgaacaa gaattctgac ctagcatcat ctcatcgtcc gagttttagc actatacaac
     4201 gtaaagaaaa cattcttagc tattggaaaa agccttaatc caacagtttc tgttttaccc
     4261 attggcgact ttcgacgttt ctgggcaaca gcctatgttt gtataggagc ctcacctaac
     4321 aaggttatta ctatattcta tttacgagtt catgatagac ttttcctaat gattaggcaa
     4381 tggaatttga aatgaaatag tgaaatttaa caatctttaa tattttttag aaaaaaataa
     4441 caaataagtt gaaagaatcc cgttgcaaat tctttcagtt gcaccaggat tctttgataa
     4501 ttttttagtt ttatgtgttt ttttatatgt tttttcactt ttcggaataa atatttattt
     4561 gttaaagtaa ttaaacaatc cgctaatcgt ttgatctggc atttctattt ccttaccatc
     4621 taaagtaaca gtagtaacga caaaatggtc ttgcaatggc ggaacttcta agtactgccc
     4681 ttgcagaaaa tcattcactg tttcatattc ttttttcatt tcttttgaat ctgtcgatgg
     4741 tttatagctt atttttaaca taactagtgc tcctttttat tcgggacgtg caatcgtgaa
     4801 aatttctcct attgctgcat aatcatttcc tgccaaacgg cttacagcat tcaattttct
     4861 tggatcgatc cttcctgtct tatcatcata gactgtttca tttaaatgat atttctgtac
     4921 ttctagcaaa aataaatcag ctgttacttt atttttattc cgtatctcga catgttcata
     4981 taaggatgtt tcaaaacgaa cttttgcttc tgtcaatgct ggtactgaaa tgattactga
     5041 atcagttaaa gtcaagtcag tcaggtacag ttcgctttcc tcattaggca gattggctgc
     5101 tgttttattt acttcttcta cgttgtcctc atcaaggata tggataacag cttctcttgt
     5161 ctcaagaata ttctttgctg tgtcttttac tgcatcttgt tttctctgga tcgccaagga
     5221 taagatgggt cgattcgaac tgacgatatt gaagtaactg aagggtgcga tattcactat
     5281 atcttttgct gatttagtcg ttactactgc tactggtcta gggatgactg aaccaatcag
     5341 caacttgtaa ttttcacgtt ctgtcaattc tgaaggactc cgttcaaaca tggtttcgcc
     5401 ttctttctat aaaattcgtt aaaaagcaga tcttgaagac aaaatgaaaa gtaatgcttt
     5461 tttcatcttc ttagaatctg ctttaaacta tctatcaatt tttctttttt gtcaagaatc
     5521 tgtgccgtgc gtgtttttta gttatttgct ttacttgcgg cttcgacata tcttttagtg
     5581 atcaattgcg gacgggtgat aatagatcca actacgactg aaaaagcacc taattcatat
     5641 gctttctcta cttgttcagg agagtcagta tgtccttcca taattactgg gatatcgact
     5701 tgttctatga tttgattcaa taaatcaaaa tcaggtgtct gcgtctttcg tgaatattct
     5761 gtatagcctg ctaaagtcgt agacaaaaga tcaaaaccat cttcttgcgc ttgaagcgct
     5821 tcctcaaaag tcgaaatatc tgccatagct aaccgaccgt gttcgtgaat catttggaca
     5881 agttcagcag ttgttttttc tgtcggacgt ttacgagaag ttgcatccag tgcaatgatt
     5941 tctgcttgcg tctccaaaag ttcttccact tctttttctg tcgcagtaat aaacacttct
     6001 gacccatcgt aatctctttt gataatccct attaccggta aatcaacgat tcgcttgatt
     6061 tcattgatat cttctgtcgt atttgctcga ataccaactg ctccgccttc ttttgcagcc
     6121 aaagccattc tccccataat aaaagaactg tgtagcggct cgttttccaa agcttgacaa
     6181 gaaacgacga ctccgccttt gatttgttcc agtgtatcca caatatctcc tccagtaatg
     6241 ttatataaaa atacagcttc atataaaaat gtaacccttt tctattcagg agacaataat
     6301 gagctatttc tttcagcaga cctttcttac taaaagaaaa ttacttcaaa ttcttcgtat
     6361 acgaccagct tctcttctgt aaattctatg ttcaacgctt ctttataagc atgtttaaaa
     6421 aactcagcat ggactttccg ccaataagct aatgtccgat ccccttctcc ttctttccaa
     6481 gcatgctctt tcgtgacttc actaaaaggc aagatggtca catttatatt tttgatgata
     6541 caaactggtt cattttttcc attcaggacc acgtctaatt gaccaacttt tggtaaaggc
     6601 tcattctctt gtttatacag atcgtaacca cttgatgtac cagtctttat cttttttaca
     6661 actagttcgc tcaactcgtc tgccattttt tctgtatccc caaatgaaaa gaaacctaat
     6721 ttttctgatt gataactagg atatttttct tggaactttt tgacatattc ttctacttgc
     6781 tgatacataa catccacccc tttgcttttt ataatgaaaa aaccatccag atactagtat
     6841 aaacaattat ctggatggtg agcataatga attattgaat attcaagaca cgcagactat
     6901 cttcaacgat ttgttttggt gttaaatgat aacgttcata aagctgccct acaggaacgc
     6961 tatccgtgaa ttctttctct gcaccaaaat ttaatacgtt cattgaagaa tcaccataat
     7021 aacgactgat attttcgcca aatcctcctg acaaactacc atcttctaat gtgatgacta
     7081 attcgtgtgt ttcttttaat ttatctaatg tttctacgtc attttctgtg atcgttcctg
     7141 gattgatcaa cgttgcttcg attccttttt ttctaaaagc ttttactgtt tcttttccta
     7201 attcaagaaa accgcctaaa gctaaaattg ctacttttcc tccattattt actgtttgat
     7261 aggctggaat actataatca tttgcgatag ctgtaccatg ttctacacca tgtgtaggaa
     7321 ttcggattgc tacaggctgc gctgtttgtt cgaacgccca atctagcata gagatcatat
     7381 cctcttttgt tgctggagca agatatttga tgttcgggat acttgataag taaggaatgt
     7441 caaatgttcc ttggtgtgtt cggtcactag ctgagatcgt tccgccaccg actaaaataa
     7501 tcactggatt ctcgttgatc gctaaatcat gtgataattg atcatatgcc cgttgcaaga
     7561 aagtacttga ttggaagacg actggacgta ctccctgaga agccaaagca gaagcgaaag
     7621 tgatagagtg ctgttcagcg atccccacgt caaaataatg atctggatgt ttttcttgga
     7681 atgttttcag tccaaaggcg cctggtatcg ctgcattgac tgcggcaata ggagctgtac
     7741 ttgtttgcat tttcttatcc aaataatcca tgatgatcat attaaatgtt tctccagaag
     7801 ttacttgctt tgcctccccg gtggacagat caaatggtac atgccaatga tatttcattt
     7861 tattatcgat tgccggctga taaccgcgac ctttttccgt atggacgtga agtacaatcg
     7921 gatgatcaat atcttttact tcttcaaata aacggatcac cgtttccaga tcgtttccat
     7981 tatccacgta acgataatca aatcccattg ctttaaatag gttattgctg cttttaccgt
     8041 tggtttgacg aagctcagct aagtttttat ataaactgcc atgattctca tcaatagaca
     8101 tctcattatc gtttactaat acgattagat tggattgtaa aacaccacca ttgttcaatc
     8161 cttcaaaagc taagccacca cttagggagc cgtcaccgat aaatgctacc acatttcctg
     8221 attctttctt cagatctctc gctttagcta aacctgttgc taagctgata gatgtagatg
     8281 tgtgccccac tgtaaaatag tcatgtttac tttcatgttg gttggaatat ccgctcactt
     8341 cacggtaatg tcctttttca aaaccatttt ttcgaccagt taaaagctta tgaccataag
     8401 actgatggga aacatcccaa acgattttat cctttggtga atcaaataca taatgaaacg
     8461 caatcgttaa ttctactaca cctagattcg gaccaacgtg cccacctaca tcgctgtcct
     8521 tttgcaagat tttttggcgc atctcttctg ccaactgttt catttcctca acagataatt
     8581 ttttgacatc tgcggggcta ttgattgcat ccaatacatg taatactgta ctcataatac
     8641 attctctcct ccgtactatc ttcttttaat gaaaatagat ttgttacact ttatctattc
     8701 tactacttag agttgactct aagtcaatag cattttaaaa aaatttcaga gaaagcttgt
     8761 tatataagta gatcttcgca cgaatcagga ctcaaataaa aaccttctac agacaattaa
     8821 aaagctttgt agaaggtctc ctaattaagg tctcatcaag aaatatgttt ttttaatagg
     8881 tgaagttcct gagcaatatg atacaaatgg atgtttgcac cttttaatga gattggatag
     8941 atctttgtct tttcagtttc taatccattt tgatagctag cagcatcgca caaaaccact
     9001 ctttttttcg cacatagaca ttcatcaggc aattcttcta atgaacgaaa taaaatagga
     9061 tttatttcag cgatgccttc ttccgcatac aaatgaaatt gggttcgata aaaaaattgt
     9121 ttgactgaac tttctgaaac aacaaaaatt tctatttcct gtctccccgc ttgttcactg
     9181 ttgatcaaag aaagactttt gacgaatttt cgaatgaaat tttcattcca tcttagttgg
     9241 atttgatttt tctttttcca ctcttccaat atttctacaa tttttccgta cagaagttgt
     9301 tctttttctg atagaagata gatcctttcc ggtaaaaata cttgtccatc tgcccacaac
     9361 gtacggacaa attgtaaaaa ggattgcttg aaaataaaat tttctgcatc gtcgatttca
     9421 gcgatactaa taatctgtaa aatcaattca ttcagtgata ggtcattgag aacaaaattt
     9481 ttataaacaa cttcgaaatc ttttcttaac agctccatgt tctcattggt ataatttcta
     9541 ctgttgaaca aagaaaaaat ataatagaca tcttcttctt gtaagtctag aattcctttt
     9601 tgctcttcca atccttgaca gactaattga aataacggct tcgtttctgc gcttttctta
     9661 tcttcttctt tcagtccaag gggaaatgcc tttcttccga ttgcgatctc tattcctcgt
     9721 atgataaagg acttttcatc ttccgaatat cttcttttaa aaaaatgagt ctctacctaa
     9781 tcaatcaact cgttacaagc ttcttctaca tacggatttt tattttcata gccttcccaa
     9841 ttgacataac gtactaaagc tagcaagatg ttccgcgagt caaattctgg gataatgatt
     9901 tctttatttt tcaaataatc attttcttta aaaaaatcca atacgatttt tttgattgta
     9961 tatactttgc tcaaagaaat aaattcaaga tcagccagtt ctgtactcgt gaatatacca
    10021 ttaaaataat aacttaaaaa ctttaaaaaa taagattgac cgtacagaaa ctgtgtgagc
    10081 tcagaaagag caataccatg tttgaatacg agtttatact tatgcttctc tcttacttgt
    10141 tcttttatac aatattctag acactcggct agactttcta gatcgtgaag gatcgttggt
    10201 gtagtcaccc ttagagaaat agctgtttgt tcgatgtcta tttcttggaa ttcaaataat
    10261 tgttcacaca gatacacttt tctaaatata tttcgctcga tgtaattttc cagcattcct
    10321 gatacacgcc tccagttttt cttatgattt tatttaatca tatctgtaaa tcgtattttt
    10381 gtttcctttt tatttagaaa agccctttca aaaaccaata aaaaaagaaa ctgggacaaa
    10441 agtcaaacaa gctcacaatt tcagcctcat tgtaggtagc tttgagccgt ttggactttt
    10501 accccagcct ctttcaagaa tggttattca aagatgtcag cgtctagttc tttgactaca
    10561 tctttcaaca attggatacc agctgcgctg ttaccaaatg gatctagtgc cgggctgaag
    10621 acacctatgc cgtaacgatt tggcacagct gccatcaacc cgccgcctac accgcttttt
    10681 gcaggtacac caacatggac ggaaaactct cctgattcat catacaaacc agcagttgtc
    10741 ataattgatt tgacgattgt agcactttct tcagtaatga tttgtttgcc attccaagga
    10801 gcaattcctt tatttgccag cacagcagct aattttgcta tgcctgtagc ggtgacattg
    10861 agggagcatt gtctgaaata agcatcgagt atctccggta cgtctccttc gatcatttga
    10921 ttacctttca tatagtaagc aagagagcga ttgatatcac ctgttcttga ttcagaatga
    10981 tagatttctt cattcaatgt gatattagga tcatcacaga tctctttcat aaatgacaag
    11041 attcgctcaa atttttcatc tgcatcttta ccggcaatca acgaggttgt tgcaatcgct
    11101 cctgcattaa caaaaggatt cattggatgt ttgcgatgat tgatttccat attcatgata
    11161 gaattgaagg caaaaccggt tggttccgtg ttgattgttt gaaagacttt ttctattccg
    11221 ttgtctttga ttgctaataa caatacggga acttttgaca tgctttcgat ggcaaagcgc
    11281 acatcggcgt ctcctccctc aatcagttgt tttgtatcca aatcataaat cgccatacct
    11341 aattgtttgg gatccacttc agctaatgct ggaatgtaat ccgccacttg tcctgtttga
    11401 taatagtgga aattttttcc aatcaattgt tctaattctt ttacttccat ttgaataacc
    11461 agctttcatt ttttattttt ctgcatgctg gaatttatga cggcatgcgt agatcgtaaa
    11521 tggaataact aagacgataa gataagaaac aatcagaatc gtttcataaa tcccatgctg
    11581 tccagctgcg atactagcag gtggaacaaa tgagatacaa aaagcaaaaa gtgaagtcaa
    11641 aagacctaaa caagcaacga ttacttttac aacttttccg cctggaacct gataggctct
    11701 ttcaagactg tcatctttaa aaaccagctt caaatagccc gcgaaaaaca taaagtacaa
    11761 catcaggtag attacaacag tcaatgacat cgctgcaaga aatgaaagat tattcccccc
    11821 gccgcttaat gtaaagacgg ctgcccagat tgttgcaacg atcccatttg cgatcatcaa
    11881 atgagcagga acaccgtgtg tgttttcttt tgcaaaatat tcaggaagta atccttcttc
    11941 tgctgctacg tgaagtgctt ttgaaggaga ggttacccaa gaagcaattt ctccgatgac
    12001 acctaaacaa acaagaatcg caagaatttt cgcaatccat gcaagatttg gatcaatgtg
    12061 tcttaatagg aattctaacg attgaatcac ccctgtatta agagacaaac catgttgagg
    12121 tactgtagta gcgacggtca atccgccaaa agtatctaaa atgattgcca gaataaccaa
    12181 tacaaacatt gctaatggat aatccttttt agggttttcc atttcatttg catgactggc
    12241 agatgtttct acgcccatat atgctaaaat aaaggaaaca aaaacaacca gcgtaggcaa
    12301 tttcgaaaaa tcaggaatca atgttttcat agacagtatc gtttgtagtg gagcgcctga
    12361 agcgacataa tggatcccta agccaaacaa gactaatgct ggcaacaaga taccagctac
    12421 aaaaccgatc cgagcaatca atgaagtgta tttggttcct tttaactgtg agagtaaaat
    12481 agcccagaaa ataatcaata ggattgccaa tttcagatat ttattgtttt gtatagcacc
    12541 aatacccgtt gcataggata atgcaccagt taagaaatac agcatggtca agtagccaac
    12601 tgttatctca aaccattgga aaaagattgc ggcaaaaccg aatcgttttc ccaatgtgcg
    12661 actgacccaa gtatagacac cgcctttttc ccatcctttc accgttgcca tctctgctgc
    12721 gcacaatgcc acagggataa accatagcaa accgcctaat aacaagaaaa atactaagct
    12781 gaagcctgag gtcgcgaatg tgggatattc atataatgaa acgaccattg agatggtcat
    12841 agccaaaaag ccaaagaacg ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat
    12901 gaatcttcct cttttctatt tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa
    12961 gtccaatcct tcttcttgtt cttcttttgt agtagtgatc gttgtaaagt gcatcgccga
    13021 ttttaaaatc actgttgtca gcacataggc atacacggca gtaatgatga caccaatcac
    13081 ttgaatccct aagaagtacc agctgcttgg attcgttagt aaatggctgt tagatgcaaa
    13141 gatccctatc agaatcgtac ctgtgaatcc gcccatgcca tgaacacccc acacatcaag
    13201 tgcatcatcc cattgtttct ttttacgaaa atcaacagca gcatgacaga cgatacccgc
    13261 aatgatccca atcagcatgg cactttttgc ttctacataa ccggcacaag gcgtaatggc
    13321 tgccaatcca gcaacggatc ctgttagtac gtcgacaaaa tcaaagccat ttccttttac
    13381 ttttgcaatg atcagccaag taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa
    13441 tgcattgact gcttgttcat ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc
    13501 aaaccaaagc agtcctgtac cgatagcagc tgccataaga ctgctatgct gacttctttc
    13561 tcctaaagga atctttcttt gaccaagtgt gaggatacat gctaaagagc cgaatccagc
    13621 tgtcgtgtgg atgactgctc caccagcgaa atctctaaat cccatctgat ccaaaaatcc
    13681 gccaccccat atccagtggc aaacagggaa ataaatcagc agattccaaa aaataacaag
    13741 tataatatag ccttttaaat tcaatctccc tgcaaaagct cctgtcatca aaggaacggt
    13801 aatcacgata aacatcagtt ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg
    13861 aaacatgttg gggaagcttg taacatgtct catcaaaaag aaatctgccg ggtttccaat
    13921 aacaccgccg atatcccgtc caaaggccaa tccgaatcca ccaaataccc acataatcgt
    13981 tgttatccca acggagagaa aagattgaga catgatcgtt agtatattat tcttccgtgc
    14041 taatccgccg taaaaaaaag ctagaccagg agtcatcagg caaaccattg ctgtacataa
    14101 aatcatgaat gctgtatctc ctgtattgat aggtaatgta ttcatttttt tcgcctcctt
    14161 tctacgatta cgagtataaa agaaggtgta gttgtttttt tcttctgttt ttttgaatta
    14221 atacttgcat ttataaaaga cagacaatta ataaaagctt gatgtgttgt gctctttttc
    14281 gtttttcaca caaataaaga caaaaaataa acggtcttct ttttat
//
LOCUS       DAYYGY010000073        13971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000073 DAYYGY010000000
VERSION     DAYYGY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.074"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACHA52_002224"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACHA52_002224"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8940898.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="ACHA52_002225"
     CDS             complement(2424..2675)
                     /locus_tag="ACHA52_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8940899.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="ACHA52_002226"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="ACHA52_002226"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8940900.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3625..3843
                     /locus_tag="ACHA52_002227"
     CDS             3625..3843
                     /locus_tag="ACHA52_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940901.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4132..4644)
                     /locus_tag="ACHA52_002228"
     CDS             complement(4132..4644)
                     /locus_tag="ACHA52_002228"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8940902.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4658..6955)
                     /gene="recJ"
                     /locus_tag="ACHA52_002229"
     CDS             complement(4658..6955)
                     /gene="recJ"
                     /locus_tag="ACHA52_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8940903.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7097..7540)
                     /locus_tag="ACHA52_002230"
     CDS             complement(7097..7540)
                     /locus_tag="ACHA52_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8940904.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7541..8329)
                     /locus_tag="ACHA52_002231"
     CDS             complement(7541..8329)
                     /locus_tag="ACHA52_002231"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8940905.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8326..9267)
                     /gene="rnz"
                     /locus_tag="ACHA52_002232"
     CDS             complement(8326..9267)
                     /gene="rnz"
                     /locus_tag="ACHA52_002232"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8940906.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9444..10031)
                     /locus_tag="ACHA52_002233"
     CDS             complement(9444..10031)
                     /locus_tag="ACHA52_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8940907.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10252..11565)
                     /gene="obgE"
                     /locus_tag="ACHA52_002234"
     CDS             complement(10252..11565)
                     /gene="obgE"
                     /locus_tag="ACHA52_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8940908.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11853..12863
                     /gene="gap"
                     /locus_tag="ACHA52_002235"
     CDS             11853..12863
                     /gene="gap"
                     /locus_tag="ACHA52_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8940909.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12948..13883)
                     /locus_tag="ACHA52_002236"
     CDS             complement(12948..13883)
                     /locus_tag="ACHA52_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8940910.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3859 a         3125 c         2453 g         4534 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atattggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaattaaa agaaaacttt ttaaaaagag
     3481 tttgagacgt tggtctcaaa ctcttttttc gcccgggttc ttcttctttc aaatttttat
     3541 cacttccctc tttacaaacc gaacaaatat tcgtatacta aatatacgaa caattgttcg
     3601 cataaattaa agaggtgaga attcatgaaa tcaatgagac gtcttggctt agtattaact
     3661 gttttgtttt taggtattat tattggtaat ttcggcatac gtccagcttt acttgtgttt
     3721 gttccgttat ttactttgtg gttcatgttg tgggatgaaa agaagtaccg tcaatctgag
     3781 cggcgcagaa ttcatgaaga tcatttctat catgagccct actacagatc caggcaatca
     3841 taataaggat tgactgctaa ttattcaaaa gacaagaagt taaactttta cgggggtgtg
     3901 acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt tctaaagcca gtttttcggt
     3961 attaagtcaa attgcggaaa aagtgatttt ttaaacttct actggtcttt gcttgtttcc
     4021 atttataaaa aaatagcgac cctcaaaagt cagatagaga atctgacttt tgagggtcgc
     4081 tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg cttaatattc
     4141 cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat ccatcaattc
     4201 gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt cgatcgtcgc
     4261 tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt gacctggttt
     4321 aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat aagtcacttc
     4381 gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga ctcctaactc
     4441 ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta ccatatcaat
     4501 tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag cgtcaccatc
     4561 tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt cgggaatact
     4621 ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac catttcttta
     4681 tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg agccgtcgct
     4741 ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt tgtaaaacac
     4801 cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa attaataatt
     4861 tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata tcaattcgtt
     4921 cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct accccattca
     4981 aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa taagaccgaa
     5041 aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga gagtttggtt
     5101 ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc gcagcttcct
     5161 ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa aggctacctt
     5221 ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact tgcagaccac
     5281 ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat tcattgatcg
     5341 aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga gtaccaaaac
     5401 caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc agatgctgtt
     5461 ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa aactgaggca
     5521 aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc tctactgtta
     5581 cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc aagtcaattt
     5641 gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt tcacttggca
     5701 tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct cgcatctggt
     5761 caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta gctgatccat
     5821 cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt ttcccagcaa
     5881 caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata tgattctcct
     5941 tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt ctttcttcat
     6001 tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac gtcgcaagta
     6061 acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg ggagcaatcg
     6121 aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct tcaaatagcg
     6181 cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa acaaggattc
     6241 gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact aaatccaaaa
     6301 attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact ccgactcctg
     6361 ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc gcataagcgt
     6421 ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca ccagcttgct
     6481 gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg atcaactgga
     6541 ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca tgctcaaatc
     6601 gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct ttcattaccg
     6661 ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt tctccactga
     6721 ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga tacggatcat
     6781 gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca tggtaacctc
     6841 tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc atttggagaa
     6901 aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt ctcactttct
     6961 cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga aagaaagaat
     7021 aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt gtacgaccat
     7081 ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat tgagtttttt
     7141 gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac tccgcctgat
     7201 aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct tcttttgctt
     7261 cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc agctgtttga
     7321 tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc agtgcaccaa
     7381 tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg aagccaaaac
     7441 ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa actaaaacaa
     7501 atccaagaat cacattgctt tgctttttca attccttcac ctactttttg ttaaaaattc
     7561 cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat gcaacttcta
     7621 aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg atcatttttg
     7681 ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt ccgcttggat
     7741 cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta acagctacgc
     7801 caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca aatttcgtgg
     7861 cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg atattaatga
     7921 tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa accatcattc
     7981 ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta aggaagtctt
     8041 cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact tcttccatca
     8101 ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg aaagcataag
     8161 cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat gccattcttc
     8221 gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt tgtgcgccca
     8281 gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg cctcttcctc
     8341 ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt tttcaaacac
     8401 ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg cactgatatg
     8461 agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt gatgagtcgt
     8521 ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc tttcatgtac
     8581 cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg tatcgcctaa
     8641 aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat tgatcgtccg
     8701 cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg gtccagctgg
     8761 aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat gttcaaccac
     8821 acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag aaaattgctg
     8881 atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat aacttaaacg
     8941 cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg gcccgtaaat
     9001 ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg gaagtccaaa
     9061 aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg gacgaatagt
     9121 cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa cttcattgcg
     9181 ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag gaacacctgc
     9241 acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt tcctctttaa
     9301 ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca aattaaaaat
     9361 aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca gcgaacccgt
     9421 ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt gtcgttggaa
     9481 gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg attttcagct
     9541 tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt aatttctccg
     9601 cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc agcaatcaag
     9661 atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac ggtctgggca
     9721 tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa ccatcctaat
     9781 aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc acccgtagaa
     9841 gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg tgtcccatta
     9901 ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc atgtaacttt
     9961 ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa aatgattact
    10021 cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta cctttaattg
    10081 aaaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg acaaaagtcg
    10141 tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt ctgtcacaac
    10201 ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg cctattcgac
    10261 aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac cgcgtgcccg
    10321 aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca ctgtttcatc
    10381 atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata agatccacgt
    10441 tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc catagtgtac
    10501 agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt ctaacaaatc
    10561 tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa ttggaaatac
    10621 tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt tgaatttcac
    10681 tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt gcggacgctc
    10741 tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt cttcataagg
    10801 atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg ttctttcgat
    10861 atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa tcaagcctgg
    10921 caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta gattaggtac
    10981 gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa cggatagtaa
    11041 agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata ctttcaattc
    11101 tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg gtgcaggatt
    11161 ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg ccacaactag
    11221 tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat ctcggacagt
    11281 ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca tccctttact
    11341 catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat ccatcaatgt
    11401 acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt ctccgccagc
    11461 cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt ctccgccctt
    11521 gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta agtcctccta
    11581 ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc ttacttattt
    11641 tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac cttcttcttt
    11701 ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat tttttgacaa
    11761 aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgagg atgccgtttc
    11821 acagcacccc taataaagga ggaacttttg atatgacagt aaaagtaggt attaacggat
    11881 ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga agtctcagat gatattgaag
    11941 tagtagcaat caacgactta acaagtccaa ctatgttagc acaattattg caatttgact
    12001 ctactcatgg tacttatcct ggcacagtta ctgcaacaga agactcaatc gtagtggatg
    12061 gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa aatcccttgg gcaaaagaaa
    12121 acggtgtaga tatcgtacta gaatgtactg gtttttatac atctgaagaa aaagcaaaag
    12181 cgcatttaga tgctggtgtc aaacgagtag ttatttctgc cccagctggt gctatgaaaa
    12241 ccatcgtcta taacgtcaat gatgatacat tagatgaaaa cgacaaaatc atctcagcag
    12301 gttcatgtac aacaaactgt ttagcaccta tggcttattt cttaaataat gaatttggga
    12361 tcgaagttgg tacaatgaca acagttcatg cctacacttc cacacaaatg ctcttagatg
    12421 gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc tgctgacaat actatcccac
    12481 attctactgg tgctgcgaaa gcaatcggct tagttatccc tgagctacaa ggaaaattac
    12541 aaggacacgc acaacgtgtt ccagtcgttg atggttcatt aactgaattg gtttcagtat
    12601 tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat caaaaaacac acagttgaca
    12661 atccttcatt cgggtatgat gaccgtcaaa tcgtttctag cgatgtcatt ggaacaactc
    12721 aaggttcgat ctttgaccca acgcaaacag aagtaacaac tgctggtgat ttccaacttg
    12781 ttaaaactgt tgcatggtat gacaatgagt atggatttac ttgccaaatg attcgcttac
    12841 tagaaaaatt cgctaacttg taatttgata gatcaatcga taaaagaagc ctttgactgg
    12901 atccagtcaa aggcttcttt cttttcatcc cctactttta aactcgttca atgaacatat
    12961 tggcttaaaa attgttggac tacttgctga tatttttcag gatctgtctt gtatgcttcc
    13021 gcatgctcgg ctccttttac aacatacttt tcttttggtc ccttcgttgc acgatagacc
    13081 ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc catgtataaa gagcattggg
    13141 cgctggtttt tcttcaattg ttccactgca tttgcttcac cgaaaaagta gccagcacgg
    13201 acttttgtca ctagactcgt tacttgcata agcggaaatg ctggaagact aaacatatcg
    13261 tctagctgat aagcaagctc atcatgaacg gaagaataac cgcaatcttc aatgattgct
    13321 ttcacattat ctggtagttt ttctccgcta gtcatcataa ctgttgctgc tcccatgctg
    13381 acaccgtata gaacgatttc ttgcgatttt ccattgtttt ctagcacttt gtttatccac
    13441 tgtacatagt ctttacgctc tggccatcca aatccaatgt aatccccttc gctttttcca
    13501 tgtcctcttg catcaggaac aaggacattg taacccatat catgaaacat ctttgcgtaa
    13561 ttggtcatcg tttcagcgtt tcccatatag ccatgagcca caagagctgt tttttcactt
    13621 ttcgtttcag caggaagata aatcgctgat aatttcagac cgtcttttga agtgatggtc
    13681 caatcgcttc ggttttcttt tgaactaaac catttatgtg catccgtttc tttggatgtc
    13741 tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct cagaaggcac aacagcataa
    13801 ttataaaaat aattccctgc aaacactaac gcaacaatca gagctaaagc gacgcctaaa
    13861 aggattttca tccaaagttt cataaatcat ccatctcatt tctctttttt catataacct
    13921 tgaattagta taatcaaaga atctctacta tgcaatgttc atttttataa c
//
LOCUS       DAYYGY010000074        13652 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000074 DAYYGY010000000
VERSION     DAYYGY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.075"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(369..1088)
                     /locus_tag="ACHA52_002237"
     CDS             complement(369..1088)
                     /locus_tag="ACHA52_002237"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8940911.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1137..2783)
                     /locus_tag="ACHA52_002238"
     CDS             complement(1137..2783)
                     /locus_tag="ACHA52_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8940912.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2962..3552
                     /locus_tag="ACHA52_002239"
     CDS             2962..3552
                     /locus_tag="ACHA52_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8940913.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3644..5149
                     /locus_tag="ACHA52_002240"
     CDS             3644..5149
                     /locus_tag="ACHA52_002240"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8940914.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5233..6585)
                     /gene="celB"
                     /locus_tag="ACHA52_002241"
     CDS             complement(5233..6585)
                     /gene="celB"
                     /locus_tag="ACHA52_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8940915.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6585..7103)
                     /locus_tag="ACHA52_002242"
     CDS             complement(6585..7103)
                     /locus_tag="ACHA52_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940916.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7119..7445)
                     /locus_tag="ACHA52_002243"
     CDS             complement(7119..7445)
                     /locus_tag="ACHA52_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8940917.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7458..9437)
                     /locus_tag="ACHA52_002244"
     CDS             complement(7458..9437)
                     /locus_tag="ACHA52_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8940918.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9458..9772)
                     /locus_tag="ACHA52_002245"
     CDS             complement(9458..9772)
                     /locus_tag="ACHA52_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8940919.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9940..10233
                     /locus_tag="ACHA52_002246"
     CDS             9940..10233
                     /locus_tag="ACHA52_002246"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8940920.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10311..11423)
                     /locus_tag="ACHA52_002247"
     CDS             complement(10311..11423)
                     /locus_tag="ACHA52_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8940921.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11576..12424
                     /locus_tag="ACHA52_002248"
                     /pseudo
     CDS             11576..12424
                     /locus_tag="ACHA52_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12777..>13652)
                     /locus_tag="ACHA52_002249"
     CDS             complement(12777..>13652)
                     /locus_tag="ACHA52_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFM8940922.1"
                     /translation="DGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVV
                     ELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGL
                     APGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPV
                     TFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGV
                     LLVFISIKFRKKRNI"
BASE COUNT         4231 a         2796 c         2342 g         4283 t
ORIGIN      
        1 tcagcgtttt ggcctgagta ttagagaaaa aaacgggaaa atagttcctc atattttgcc
       61 gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaga ggttgtgaat
      121 tcagcgtttt ggcctgagta ttagggagat cgtgtcctta ctatctaact ctgagtcaca
      181 aggaacaatg gggactgtaa caaaagtctt gtcacagtcc cttattccga acaaacggtg
      241 ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaaagcca tccgaaagtc
      301 agacggacaa gtctgctttt cagatggcgc tacaactact ctgcttattc ttttagctat
      361 ccattcaatt atttttctct cacttgttct agctcttctt tggtcatggg tcgatattgc
      421 cctttattca aatctttagg cagccaaaat tttcccatac ggattctttt taaatataag
      481 acttcttttc ctactgcttt gaccattcgc ttaacttggt gaaactttcc ttcatgcaaa
      541 atcagacgga taattgaagt ttctgtctct tgatctactt gatcaatttg gagttttgcc
      601 ggcaatgtct gctcatcgcc atcaataatc agtccttctg caaataaacg gatatcttct
      661 tctgtcatta taccgctaac ctcagctaga tattctttct ccacatgttt tttaggtgat
      721 agtaactgat gggcaaacac cccgtcattc gttaaaatca agaggccctc tgtgtcttta
      781 tctaatcggc cgactggaaa aagatcttcc cgataatcct ggtcttccaa taaatcgatt
      841 acggtttgat catgattatc ctgagtagca gaaatgactc ctgcaggttt atgcaaaatg
      901 tagtaaaaat ctttttgata cgtaatgatt tctcctaaat aagtaaccgc atcttcgaat
      961 tcatttactt gcgctttatc gcttttcacc acttgtccat tgacttcgat cgttcctttt
     1021 ttgattaatc ctttgacttc tttgcggctg ccaagtccca tatctgctaa aaacttatct
     1081 aaacgcatgc gtttccccca tttcaaaaga gcatcaatct agctcttctg attcacttat
     1141 tttattttta atttatgtcg taaacgcgct attcctggtc ctagtagtct ttctgccaag
     1201 cggattttca aagctaaata accatacact aaagccccta tacctgctac tacaagaatc
     1261 aacaatagag actggaattt gctatcttcg tttaaaaaag caccaaaaat catctttgtg
     1321 atccacgaag ccaaaagcat aatcaaggta agaataaaga tcagcaatgt cctgcgccag
     1381 acaaaacttg gtttaaagcg ggctacttca tggattctct ttaagatcaa ggtacaagac
     1441 acagcaaatc cgatcattgt cgccaataaa gggccgtata cttcaaataa acgaatggct
     1501 gggtattgaa gaaggagttt cactagaaac ccaataacca aataattaat tgccgccttg
     1561 ttttcaaaca ttccttgcag catgttggaa actagcatat atagtcctag gaagagtcct
     1621 acaaaacttg cttggatcag tacattactt cctagagaat ccggtgtata aaataatgta
     1681 tttagcggat aggcaagtaa cataacacca aatgtcgccg gaaacataaa aaatgaaaac
     1741 aactgtaaat ttccgcttgt caattttgcc agacctactc gatctttcaa cgtaacagct
     1801 tctgtaatca acggtaaccc agtagacgcg atcgatgttg ccaatgcaat aactaccatc
     1861 gtcaacttat ctggattggc actaaaaatc gaaaaaagat ccagtaactg tgcattggaa
     1921 tactcggtgg atgcagacat gatcttcata aaagtgaact gatcgactaa tttgaagatc
     1981 gttacccctg aacccacaat aataaacgga atcgcttctt taacggtatc taacaacaat
     2041 tccttcgttg caatatttat tttgttttcg ctgtagtgaa taaacgccga tgtatacgct
     2101 tgatgtttct tcaaaaaata caaaaggaca agaatacttg ctagcatacc gataaatgcc
     2161 gcgaaagttg attgcgtgac cgccgtcaca tagtcaccat ccatgacttt catgatgatg
     2221 aatgtagcca atagcatata aaaaatacgt gccacctgtt ctacaatctg cgacagcgca
     2281 taaggcatca tttcttgatt gccttgaaaa taccctcgta tcacactcat acaaggaaaa
     2341 accaatacag cgatactcaa agaacgcatc gttggaatca gttcttctcc gccaccagaa
     2401 gcatgagcta gccaaggaga ggcgatgtac atgaacaaag caaacaacag gcctaacccg
     2461 cccatcatct gtaatgcacg gataaataat cgtcgtgaag tgccatattc attcaatgaa
     2521 ttatagcgtg cagtttgttt tgcaatcgca gcaggtattc ctgctgtaga gatcagtaga
     2581 aacaatgcat atatattgta tcccatattg aataatccat tggcagcctt agcattttcg
     2641 cccatccaag cataccaggg aatgatatag acagcgccta gcaatcttga aatgatatta
     2701 ctggcggtca tccaagcaga gcctcgtgcc attttttctt gcggtgttaa ttgatctaat
     2761 gctggttttc tttgattatc catcggtcag attccaactt tctacaaaac attactatca
     2821 ttttaattgg taaaacagaa actggcaatc gattttatat aacttttata agaacaattg
     2881 tttttttgaa ttcggtttcg tttttttggt aaagtaaaga aatatacagc cttttatggc
     2941 taacatttat aggagatatc aatgacacaa gaaataaaaa aactacccgt tatattttta
     3001 ggtcttttct ttatcacagt tggattgaat tggttcttgc tccctcacga tatagcttcc
     3061 gccggagtgg gcgccatcgg acatttagtt gaaacggcct tttctatcca acgctcagtt
     3121 actgtttgga gcatcaatct tatgatgctg cttctagccg ctgtcctatt aggaaaatca
     3181 gtatttttta agactgtagc gggaagtctg ctttttcctg ttgttttaga gattgttcca
     3241 aaagtggaaa tgatctcctc acatttcttt tcactgattt caggaagtct tttgttcagt
     3301 cttggggttt acacattgta cacagtcggg gcctcaaatg gcggtataac gataccgccg
     3361 attattttcc acaaattttt tcggcttcca atgccgcaag gacttttact taccaatagt
     3421 cttatcgtct ttttgaatta ttatgttttt ggattgttgg agacactatt cgttctccta
     3481 tctattacag tcagttcatt gtttatgaaa atactcatcc gcttgtcccc tgtgtcaaga
     3541 aaatttgaat aaagctggat tttgtcatag cggaaacaaa cgatatatta aaaagagtgt
     3601 gctataataa accaagaaat tgaataaagc gagggatcaa tcaatgagct tctctttaga
     3661 agacatccgt catttactat taaaagaaaa tctattaaaa gaatttgttt caacaagagg
     3721 ctggcatctt gatgtaccag aaaaagctga atttcttcag ttaagttatg attcaagaaa
     3781 agctgatgct tctactttgt ttttctgtaa aggaatgaat ttcaaagaag aatatttgga
     3841 ttcagcaatc gaacagggta ttcaatatta tgtatcagaa caaccatacg aaaattcagc
     3901 agtaggaatc atcgtaacgg atatccgtaa agcaatggcg cttcttgcta tggcttttta
     3961 cgactatccg caaaataaat tgaaagtgat cggattcact ggaacaaaag gtaaaacaac
     4021 tgctgcgtac tttacaaaag caatcttgga tcacacaaca aataaaaaaa cagcattgct
     4081 ttctactatg aatacaacgt tagatggcaa aacatatttc aaatcccatt tgacaacgcc
     4141 tgagtcatta gatttatatc gtatgatgtc agaagcagtt gaaaatggta tgactcatct
     4201 agtgatggaa gtttcttcac aagcttacaa aacacaacgc gtatacggct tgactttaga
     4261 tgtcggaatc ttcttgaata tctcgcctga ccatatcagt ccgatcgaac atccgacatt
     4321 tgatgactat ttctattgca agcgacaatt gatcttgaat tcaaaaacag ttgttttaaa
     4381 tcatgaaagt gactattttg atttacttag ggaaacagcc gatctattcc agattccaac
     4441 gatcacttat ggtcgttcgg aagaaagcaa ttatcaagtg atccgttcag ataaaggtac
     4501 tcacggtttt actttatctt ctcatgaaga tcgcttagct atctgcgata ctgcttatga
     4561 tattttgctg gctggaggat tcaaccaaga aaatgcagcc agtgcgatca ttgccgcagc
     4621 actaacaggt gcctccagtc aagatgcaca agacggatta aaagaagctc gtgtaccagg
     4681 ccgaatggat caattgatcc aatccaatgg agcgcatgtc tatgtggact atgcacataa
     4741 ttatctaagc ttgaaaacat tgctgaaatt tgccaaaaat gaacatccag atggccgtgt
     4801 aattgttgta ttaggcagcc caggaaacaa agcaatctcc cgtcgacatg attttggaaa
     4861 agtgctatca gaaacagctg acatcgcctt tttgacggca gatgacccag catttgaaga
     4921 tccgaaaaaa atcgcggaag aaatcaatga agcaattact aatccagatt tgatcgttca
     4981 ttatgaaatg gatcgtcctg aagcgattcg acgcgcttta gcagaaagcg ctccgcaaga
     5041 ttcagtcgtt attgcgggaa aaggcgtgga tccctatcaa aaaatcaatg gtgtagatga
     5101 accttatgaa ggggattatg caatcgtgaa gcggttgatt gaagaataat ggtatttata
     5161 tactagtggt ttcccatttc taagaaaaaa agggactgtg attttttgtc acagttcctt
     5221 ttttcttaga aatcatttta acttttcact agctaattct gctgcctttt ctctttcttc
     5281 atttaataat ttattatcat agaatttgat aaatgggaac cagataatga atgcgatgat
     5341 tgcacaaatg actgctaaca cagcagcttt ccaatcacca ccactaccaa caaatgctcc
     5401 gatcccgact ggtgaaggcc acggcatctg tgcaagcatc ggtttgacga tttccagccg
     5461 aatggcaaaa taagcgagtg tagcagaagc catcggagct aagaaaaacg gaaccgctaa
     5521 atagggattg tagacgattg gcatcccgaa aatgattggt tcattgatat tgaataatcc
     5581 tggtacaagt gaggcttttc ctaacacttt cagctgatca gatttcgcga ggaatgcaat
     5641 aaagatgatc aatcctaaag tcgcacctga tccaccaacg gttacataag aattgttgaa
     5701 ttctcctgcc aacggataat ttgcgccttg tgcgttagct gccatattag ctaatacgat
     5761 cggggtcaag aaagaagtga tgatattcgc tccatggatc cctacgatcc acaatgcatg
     5821 gatcaagaaa taaatcacca tgacaccaag ccatgtattc gtcaactgcg taacaaaccc
     5881 aaatgggata gcaatcattt tgaagatatc cgttcctagt gcaactaata atccattgat
     5941 gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga gcagtgaaag aacgagaaac
     6001 tcctggcgga acaacatctg gcatttttat gacccaattt ctcttgacac acatacaata
     6061 caattcgacc gcaataatcg acataataat agctgtaaag attcctgaag ttcctaaacg
     6121 ttcgacaaaa gagcccatcc taataccatt tactacagtt tctgtctcat tgatagaagc
     6181 aatcaagctg attttaccat cagtcatgat caattcaggg atacacataa agaatgcaaa
     6241 aacagatagt aatgcccccg tgagcggatt aacatttaat ttttgttcat ctcgttcaat
     6301 tgacgttaat tcataagcaa acacaatggc aaagtaaata gataaaatcc ccatcgtcat
     6361 tgtattgacg atcatgtaca agtcactaac tttgaaaaaa gttgcattaa aaaatccttc
     6421 taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa aacatcgagc ctacaatcgt
     6481 gaaaggaata gaagccatcc ccgctgccat gacagcccga acgattttaa gttgcgcaat
     6541 tttccccata ggtcccatca ggtatttctg caaaaaatca aacattagtt attctccttt
     6601 cctaaatata acgctttttc atattgttta atacggatat actgttggtc ttggttgagt
     6661 gaaattttct ttaaacgata ctgtataaat gcactggcaa aaataccgct tacaacaaga
     6721 atgaaaacaa ataactgtcc tccattgtta tttgccataa aaggatacaa gaatgtaaat
     6781 cctgaggtaa acaacactgg aatcactaat aaattaaaga cactctggaa caaaaaatac
     6841 cactttgtcc atggaacgtt cgtttggtgt tttcgataaa gctttacttg ttctcctact
     6901 gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac ttcctaaact tttagctgat
     6961 agaagcagaa tcatccattg catgttcatg aaaaagaaaa aggcagtcag gtaacgtacg
     7021 attagataac gattgaaata catgtttttc aaactaagtt gctttttttc taattcgatt
     7081 ttccttttta ctgtatcctg cacaatgatc cttcctttct atttattttt ttgatacaaa
     7141 taattcattt cctgtgcaac ttccaataat gtcatcgtcg tcatcagatg atcttgtgca
     7201 tggaccatga taatttccat ttctattttt gtcccgctgg catattcttt cagaagttga
     7261 gtttgtgact gatgcgcatg aagaatctct tcatttgctt gtcgcaactt ctcttctact
     7321 tcttcaaaca tctctttacg catcgagcga aatgcttcat gtatcagtgt ccgtgcattt
     7381 ccgctatgga aaatgatatc aaaagaagtc acttgtagtg tttcactgtt tattttttca
     7441 gccatctgtt ttcctcctta ttcgatcagt ttgataaatt tactgctgaa attctcaaaa
     7501 ataggttcag ctaagatttc ctgctgtact tttagctggt caactaacct aacgattgct
     7561 ttagtcacga ccgtaattcc ttcattttcg acataagaag gcgaaactaa aaaaacaaaa
     7621 tgaatttctt gatattgatc ccactgcatc ccagaaggaa tcaatgcaac ggctaccttt
     7681 gtttcgattc caactggaac agcgggatgt gggactgcca ctgtatcact gaatatgatt
     7741 tgtcccatat tttcacgttg ctggatctgc tttcgcattt ctcttatata tccttcttct
     7801 tcatttatgc tcagcttttg cagcaaatca tgaagaattt cttccttttt cggttcgtac
     7861 gtataagtct tgaaaaattt ttcagacaac tgttcggtgt aaatctgctt tttttgctgt
     7921 agagaaaatg ctggtatcgg tctatctgag gaatgatagg gtatagattc tccaactacc
     7981 tttcgtattt tacgaacatc atcttcattc aaaaaaatac tgacatgtaa aactggaatt
     8041 ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca ggtcgattcc ttttaaagtt
     8101 gtttcattga tctcataata gccttttaca cttaccactg taatattttt accgaattca
     8161 ctgactacgc gattctttaa tagttgggca ctgccgtaac ccgttgcaca gataatcaat
     8221 gcatgtagtt tatgcgcagc tctttctttt tccaatgccg ccatgagatg caaagccaaa
     8281 taggcccatt catcttcatt gatggtatag tttttcaaag ccggcatgtt gctgaaatgc
     8341 ctttttactg cgtcaaagac tttgggattt tctttcttta tctcctcagt caatgggttc
     8401 tcaatgcgaa tacctcggct caatcgaatc atcatcggtg acaaatgatc gagtagtcca
     8461 ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt cctgagacaa tcttttcaga
     8521 actttcgcta attcctttga tagttcctga tcattttgat tttctttgtg attcgactta
     8581 gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg gaaacgatac acctgcgatt
     8641 acctcaatac gttgaacgat ccgctgcgcc acttgatact ctactcgctt agaaacttct
     8701 tgttcgatgc ctaattcctt gatttcaaac ccttctcgga gccgtttgat ccccaaagcc
     8761 aagtgtaaag tcagattctg tatgataaaa tccgaaactt ttaacttagc ttctcttgtt
     8821 tcatcgagaa tgataatgac tagctcttca aaactgatgt ctgggaaaaa acggctttgc
     8881 cctaaatatt ctttcaatga atttccataa tggtttccaa aaaaagtatc catgataaaa
     8941 tgccgtcgat ttctttcttc cccttcgatc catgtccctt gaccatgctt agaaacgatt
     9001 tttagtgaat atggaatcaa tcgctccttg atttcttgca tgtcctttgt caaactggaa
     9061 cgactgatat aaagctcttc tgccaaatca tccataaaaa gaatggcatc ttccaaaagc
     9121 aattggttca atatatactt ttgtcgatct tcggactcat aaaatccttg cggttctcta
     9181 gcatatccag gtactatttc ttgccgactc aaaaacaagt caaatgtcag tctttgtaaa
     9241 atatgcagtt ggtagccata acctggttta gcaatgatct tacctccgtt tttttctact
     9301 aactctttga tccgctgcaa gtaattacgg attgtccgat cagataataa caattcagaa
     9361 gctaaacgtt gactggtgac atactccttc ttgtgatcaa tcaaatatct caacagcaat
     9421 ttttccttct gtttcattat catccactcc tattccatca tatgttctct tcgatcacct
     9481 ctgctaactt ctggatgcct gtagggattg ggacatacgc ttggaaaggg atactaacaa
     9541 caggctttcc aacagattct cctaattttt tgaatttatc caaaaacatc gtcgtttgcg
     9601 ggctgatcag aaataaatca aaatcactat tcttaatcat attatcgcct tctgtagctg
     9661 aaactgcatc tacgagtacc tcttctcttt tttgcttaaa gaaatccgtt gtctttgagg
     9721 ccatcatcga ggatgacatt cctgccgcac aaataatcaa tgcttttttc ataaatcaat
     9781 cactcctttt atttttataa accaagcata tcttgtaagc gttttctatt caaaatatat
     9841 atttccgtag cattgcggaa aaaatgtgta tcatatcttg aataataatt tctggcaccg
     9901 tgatatacta gtgatagaat tttttcagga ggggatttag tgagtatcac agtgaatatt
     9961 ttgtatacaa gtacaaatgg acaagccaga aaatttgcag aagaaatggt cgaaagaaac
    10021 attgtcaaag cgattcgtgc agaagaagga aacgagaaat atgactatta ctttccacta
    10081 gaagatccag aatctctttt actgatcgac cgttggaaag atgaagaagc aatcgaaaag
    10141 catcacaaat ccgaaatgat ggctcaaata aacgaactcc gtaaaaagta taaattaaaa
    10201 atgaaagtcg aacgtttcac tgatttaccg taaaaagata cacatggaat gatagcaatt
    10261 tatcatccat gtgtatcttc actttttcct tattttttat tcgatctcat ctaatgattc
    10321 aaaccgtgtt tttctcttat ttttcggata agtatttcag tcaccccaat atacatgatt
    10381 cctaaacata ccccaaggat attctcaaaa atgcggatcg ttactgctcc tggaaccccg
    10441 aataaactgg cagtgattgc caaagcacca aaagaattaa aaactgtttt atatctatag
    10501 gtagaacaaa cacctaaagc tagaccacct aatataccga cccagttaaa aggaataaat
    10561 tggctgatac caataaataa aacacatcca atcaatgagc caatgattcg gtctactgct
    10621 cgttctttga aaccaatcga catcaatcca tagctagata caatagaaga aaatgcaaac
    10681 cctgcccaca tcaatcgctg aaaagggaca tactctcctg caacgatcaa aagactaatg
    10741 ccaaatgcat agatgagcat ccaaattttt tcctgcgaat acatgccttt caaaaaaatc
    10801 tctttaaaca agcttttgtc tttgttcttt tctcgatgtt ttctgtaaag gagaactgaa
    10861 aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg taaagaaatt cttgttcaac
    10921 gaatctttag gtaacgaata aacaataaag agatatgaaa agccgtacaa agaagcattc
    10981 cccattttgg gatcactagc ggttgccatt aatatgctgc ttagtaaaat aaaatgaatc
    11041 aacatgccca taactgacca tttgatcagc tggacatacg gtgctactaa tagtgagaac
    11101 atgacgattg ctaaacctat gatcgaatgg gaaatcttat aaccaaagtc aacaaaacga
    11161 atactcatca acatacagaa taaaaccaca gcgtatggtt tatgcttcga ttcaaataat
    11221 gttgtaatta taatgataaa acaaatggca aacaatacga tgaataatga tcgtaaaaac
    11281 aatgaacaaa tatacatgca cttttctttt ttagaagctg cttctcgtat tttttgtttt
    11341 aaaataaacg gatctagttg tagagactga taaaaatcca cctgttgtcg tcctttcttt
    11401 ttacttttat tttttacagt catctatgac aggttgtaaa tactatggaa catcatatgg
    11461 cgttgtcatt ttcttgtcaa ctatttttcc tattcatgtt tttataaaaa aacatatcgt
    11521 tttcttgtat gattaaatca ccacaaaata aacaactgag agaacgatat gctttatgaa
    11581 tggttctatc aaaaaaattc tgagatcaat agacaaaaat ttgatgatta cagaggtctc
    11641 ttacgaaacc ttacagaaga aaaaaacatt gatcgttgct gcagttctct tccctgctcc
    11701 atgtgtctgt agaaattgtg gttctgctgt ggtagatgga aataggaaat taatggtggt
    11761 gaaaaacaag aagaaggaaa ccattgtcca ctttgaataa tataatcata tgccaatggt
    11821 tatacgccta aaaaagcagc gctatacctg taaaaactgt cgaagccatt ggaacgctca
    11881 aagttatttt atccgtccaa ggcactcgat ttcaaatcat gtcagacata aaatcacttc
    11941 tttactgact gaaaaagtgt cgttattttt tatttcgaaa agttgttagg tatctttgac
    12001 aaccgttatt cgcacattga aagagttgag aagctattta ccaaagtctt ccaaaagaat
    12061 tctgtcttga gtgttgaggg ttgtgaattt cgttctcacg tttccgttga ggataaactg
    12121 agttttattt ccgcggatgg cgagacagaa aaattaatcg agaaaattta cctacacaaa
    12181 aaattcctcg attaacaaac tatttcctta gctgcactaa tcctaaagaa atagaattct
    12241 tagttacaga catgaacgcc acctactttc agctcaccaa acatgttcta ccaaatgcga
    12301 aattggtgat tgatcgatta cttggttttt ccacgccttt caaggaggcg taacctttat
    12361 tcacgagttg gttgaagctt tccgagacaa aagacccgac ttgtttttct ccttattgac
    12421 aaagagccat taaaactaga gaaaaaatta caactgatcg ctgattattt gtagaaagaa
    12481 aatcaaagta ttacccaata aacttgagtc attaggcaaa aaacactgcg aaactcaaga
    12541 atgattttcg cagtgttttt gtatattttt tattaactag tattactaca aaggtttatt
    12601 tttcacttct caacgcagca caaaattgtc tttacttatt tactggttgg gttcaacctt
    12661 ctctactccg atactaatat ttgtttgtat accccttcta aaatcggtag tttccattca
    12721 attgatgggt actagttgat tttaaaaact atgcttattc aattttttat tttagctcat
    12781 atatttcgtt tctttctaaa ttttatactg atgaaaacaa gtagcacacc caatattgac
    12841 aatataaact ggctaatgat ggtttctcca gtttttggaa gatgcttatt tccactgctt
    12901 gttggattgt ttcctcttat atctgagcta tcagcaactt ttctattctc ttttgttaaa
    12961 aagatcgcca cttcttgttc tttttcgatc ttgaaagtta ctggtgtcgc atccaattca
    13021 taccccgttg gcgcttgagt ttctaccagc tgatatgctc ctggtgctaa gccatctatc
    13081 gccagttttc catctgctcc tgtggttaga cctgactgca gaacggttcc attggcatct
    13141 tgtaactcaa aaacagctcc ttgcaggact tctccgcttt ggtcatcgat ctttgtcaac
    13201 acgacaccgc ctggtgtaag tcgattttct ttcgtcaatt ctaccaccgc tgtctgactt
    13261 ctttcgatct caaactcgat tggtgtcgca tccaattcat accccgttgg cgcttgggtt
    13321 tctaccagct gatacactcc tggtgctaag ccgtcaatcg ccagttttcc atctgctcct
    13381 gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa aacagctcct
    13441 tgcaggactt ccccgctttg gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt
    13501 cggtttgctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc aaactcgatc
    13561 ggtgtcgcat ctaattcata ccccgttggc gcttgagttt ctaccagctg atatgctcct
    13621 ggtgctaaac cgtcaattgc cagttttcca tc
//
LOCUS       DAYYGY010000075        13556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000075 DAYYGY010000000
VERSION     DAYYGY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.076"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..1656
                     /locus_tag="ACHA52_002250"
     CDS             19..1656
                     /locus_tag="ACHA52_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940923.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1801..2541)
                     /gene="truA"
                     /locus_tag="ACHA52_002251"
     CDS             complement(1801..2541)
                     /gene="truA"
                     /locus_tag="ACHA52_002251"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8940924.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2732..3367)
                     /locus_tag="ACHA52_002252"
     CDS             complement(2732..3367)
                     /locus_tag="ACHA52_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8940925.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3514..4734)
                     /gene="sstT"
                     /locus_tag="ACHA52_002253"
     CDS             complement(3514..4734)
                     /gene="sstT"
                     /locus_tag="ACHA52_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8940926.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4953..5951
                     /locus_tag="ACHA52_002254"
     CDS             4953..5951
                     /locus_tag="ACHA52_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940927.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6147..7982
                     /locus_tag="ACHA52_002255"
     CDS             6147..7982
                     /locus_tag="ACHA52_002255"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8940928.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8042..8758)
                     /locus_tag="ACHA52_002256"
     CDS             complement(8042..8758)
                     /locus_tag="ACHA52_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8940929.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8985..9527)
                     /locus_tag="ACHA52_002257"
     CDS             complement(8985..9527)
                     /locus_tag="ACHA52_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8940930.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9532..9759)
                     /locus_tag="ACHA52_002258"
     CDS             complement(9532..9759)
                     /locus_tag="ACHA52_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8940931.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9838..11718)
                     /locus_tag="ACHA52_002259"
     CDS             complement(9838..11718)
                     /locus_tag="ACHA52_002259"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8940932.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11907..12521)
                     /locus_tag="ACHA52_002260"
     CDS             complement(11907..12521)
                     /locus_tag="ACHA52_002260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8940933.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12663..13223
                     /locus_tag="ACHA52_002261"
     CDS             12663..13223
                     /locus_tag="ACHA52_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8940934.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         4248 a         2634 c         2139 g         4535 t
ORIGIN      
        1 tttaaatagg agaaaaaaat gaaaaaaaac aaaaaccaaa taatagatat catttttatg
       61 ttatttctaa gttttgctta cgtcatccct ctattcatgt ctaagggaat ctatcatagt
      121 gtcaatcagg atacttactt tcatttatct cgaatcatag gactagataa tgtatggagt
      181 tctccagtaa acttcaataa cttcgatcat catggaacaa tgatgaatat tttttatcca
      241 tggcttactc tttatccagc ttttttattt tataaaatga tgggaaatct tgttttaagt
      301 tataatatct actatttttt tatcactttt ttaacaatgg tcgtttctta tttttctatg
      361 aaacaaataa aaaacaatcg ttatatttcg ctgttatttt cgattattta tacattttcc
      421 gcctatcgtg ccattgacat attccatcgt gcttcattgg gagaagctgt cgctttaaca
      481 tttttgcctt tgatactaat ggggtgttac gaaatttata ttagagatta ccaaaaatgg
      541 tactggttgt ctattggcat gacgctagta gtgtatactc atttactttc tgtcgctatg
      601 gtttctgtat ttattggagg aacgttgttt ctttcttttt atttttggga ccaaaaaatc
      661 gctagactct taagtctttt aaaagctacc gtcctcacct tttttttaag tgcgggattc
      721 ttaattcctt ttattcaaca atctagagca caagaattga aagtcccact gggaaaagaa
      781 ttaagtggta tggctcctag tgatatgctg acacatattt taaataataa ctataacaac
      841 tatactattg gattattctt atttctcgga ttgattggtg cttttatttt tataaaaaaa
      901 ttaactgctg atgatttatt tattttcttt cttggtgtat ttgttctttt ttgttcaacc
      961 aatttatttc cttggcaact ttttaaccat acaccagtta agagtctaca atttgtctgg
     1021 cggttaaatg gtttttcttc tttatttata gcctatacga tgagtatagt gatctattat
     1081 attttttcta ctaaaaataa ttcatggaaa ataatggtct ttacgtttct tgcactaata
     1141 ttgcatctct caggagtaag caattctatc catgctaaca aagattcatt aacacttata
     1201 gcacctgaag acgctgttgc tatagctgaa aattacaatc atactgatta tgcaaacaaa
     1261 gagtcaatct atcatcctga tatgattaat aatgatcaat atttactagg taaccaggaa
     1321 ataaatccaa caactcattt tatgagtaac aaacttacaa ttgaattaga taattcgaat
     1381 aataaaaata ctgtcttgac gacacctatt tatcgttata aagggcaagt ggcttctatt
     1441 aatggcaaac tagtacaaac aaaattatct aagtttggca caactgaatt aactattcct
     1501 ccaggtataa ataaagtcgt tattacttat cagtacacaa aactagcaat tgcttcaaga
     1561 tatttatcta tcgttacttt gattttattt ttactttatc gatttacttt ttctaaacaa
     1621 aaacaaccga gaagagaagt acaacattct cactagattt ttatttaaga aagacttttt
     1681 agctgtacac ataaacaggc taaaaagtct ttttttatat ttctttaatt tgcttcatac
     1741 attatttaac atcgatattt atactgtgaa acatgcgttt catccattat caaagtattt
     1801 ttatcgataa taaacttcat ccaaaaacag accttgtgca gggacggttt ctcctgcatg
     1861 ttgccgcact ttgctttcaa atatttcatc gattgtttct atcggcatgg cacctgtgcc
     1921 gatttctagt aatgtcccca tgataatccg caccatttta tataagaatc cgtctccgac
     1981 aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat aattcatcaa tcgtacgcac
     2041 tgtggatttt tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg tacccactaa
     2101 tttttcgcaa gctgcattca gcttgtccat atccaactct tgagggaaat aaaagctgtg
     2161 gtttcgctca aatgctgaag gaatcacgtt gttccaaaca taatagctgt attttttgcc
     2221 caccgcatta tatcttgaat ggaagcgctc aggcatttct tcgattcttt tgatgacaat
     2281 atcccgcgga aggtaacgat tcataaagtc gatcatttct gccagtccaa tttctgtgtt
     2341 tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca gcatctgttc ttcctgatcc
     2401 aataatttct atctcttcat ttgtcatctg atgcaatact ttttcgattt ttccttgaat
     2461 ggttttatcg tcatctccaa gtctttgcca tcctgaatat ctttttccgt catattcgat
     2521 tgtcaacttg atgtttctca tattttagtg gctcctctgt cttccatttg gtcttctctc
     2581 taccttttta agattgtagc acaaaatata gctccttgct tctctaagac agaagctata
     2641 caagaaaact gtggaaatat atatttcaga aaaacaaaaa acagccaccg gatgaagtat
     2701 ccgctgactg ttcaatttga cggctcaagt tttactgttt gagatgcaag atactcaacc
     2761 aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaaacct attttattca
     2821 gtaatggcca agaatttata gaaggaattc ttgccagtcc ataaatcgcc gcaaaaagca
     2881 aactgatcac agacagtcca aggtataagt ggcttacgtc catttgccca ttttttcgat
     2941 agatcaagaa aagaagaact ggaaacaata aaaaaccact gtaacctaaa cctgatccga
     3001 tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata ctgaacaaac
     3061 ctgtaaagat acaatcacct ataccataga acgcgatggc tgctgtaact agggtagcta
     3121 acacacgaga tgtactaaca aatgcagcat acaatgcagg caatgacagg atgaaaaaga
     3181 tccctgaaac cacagaccaa ataagaaacg cttttcttac aggactggaa acctctccaa
     3241 atacactgat cacgctcttc atttgatcta attcaggata atacaatcct aatacgtacg
     3301 gagtcaaaaa atcactcagc acgcctaata ataaaaagaa aaaaccaaat ttacgtaaaa
     3361 aatccatgat acgcctccaa taacaattac ctatgctttt aattgttatt ataaacgact
     3421 ttatcttgat ttaacagaaa cctctattta cacgaacatc tatataactc attaaaaact
     3481 cgaatcattt taaaaatact aaatatctaa cgttcaacgt tcacttaatc catctaaaca
     3541 atctttcaat ttgatttttt ccccacttct tttacgcgcg cctagctctc ctattgcagt
     3601 aaacaacaga tcacttgacg aatttaaggc agtttccatt gaatcctgga taacaccgat
     3661 aataaatcca ataccgacga tttgcatcgc gacatcatta gagataccaa acaaactaca
     3721 tgctaatgga atcaataaca gtgaaccgcc cgcaacacct gaagctccac aagcagctaa
     3781 agcagacaaa atacataaaa ggactttcaa aacaaaaggt gcttcaatcc ctaaagaatg
     3841 tacagcagtc aacgtcatca aggaaatggt aatcgcggca ccgcccatat tgatcgtagc
     3901 acctaacggg atcgatatgg aataagactc ttttgtcaat ttcaatcttt cagccaacat
     3961 catgtttatc gggatatttg cggcagaact tcgggtaaag aaagcgggaa gtgcgctttc
     4021 tttcaaacaa aagaaaatca atggataggg attttcttta ataaacaaaa acgtgaggaa
     4081 tgggtacaca actaatgcga caaacagcat cgtaccgatc aaaacaagta aaagctgcag
     4141 atactctgcc aatccagcga ttccagtcgt agcaattgat tgataaacca atcctaaaat
     4201 ccctatcgga gcaaatgcaa taatcacttg tacgacttgt gacaacgcta tagatatctg
     4261 atccaccaac tcttttgtcg attctgaacg tgctcgaaaa gctagtccca acaatacagc
     4321 ccagaataag acgccaaggt aattcgcatc aataagagat tgaacaggat tttgtgtgac
     4381 attcgtcaac atagtagata aaacactttc caaactttgc ggtgcttttt cagatactgt
     4441 ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca ataactgcta caagtgcaga
     4501 aagcaatgtt cccactaaat ataagatcaa aataggcttc acgaatgtct tattcccaat
     4561 tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg cgattgcctt
     4621 caagcaactg acaaacaacg ttcccaatat tgaaataaat gtccatgtgg ggaaaaagat
     4681 accaaacaat attccgacta aaataccaac aacaatacgt tgaactaacg acatatccaa
     4741 aaacttctct aacaaatgac caccctttca caattttaac aatcattgta tcattttatg
     4801 caaagaaact aaaggtataa ttttctaaaa attctaacaa tacaaacaac catttcttgc
     4861 taattttgta ctttttctgt ctacacgttg ttttttgaga tattctaagt taggatgtta
     4921 ttaatgggca acttatcagg aaacgttttc ttatgttttt gtccaatttt tttgtgaggt
     4981 gtttattctt gaaaaaaata aagaaggaga gttatggctt cctgacaatc tttgtcgctt
     5041 gtataatgat ggttttacag cgtatattga tttcagcagt cactattcct acatatgcaa
     5101 caaaccctgg tcgattctac atctatacat ttttacaaac ttccgttgtt attggtgcaa
     5161 atttgatccc actttacatg ggttaccaat atcaaaaaat cgcccgttta aaagttttac
     5221 attatttaag tcgttttgcc tttatattcc taatagccac actagtttgc aatatctttt
     5281 tttacgtaca agctggcagc ttgaatattc gtgattattg gcttatccta acacctatca
     5341 gtcagaatta ctttacttat gctgtttctt gtactttgct tttttgtagt attccttata
     5401 cagttggatt tttaaataaa ttatcgaaaa caacaattaa atatctacta ttatttttaa
     5461 caattggatt agttggctgc tctactctat ttaataaaga cccgtttgca ttacagaatg
     5521 gacagagtat tttatggatt ttttatttat ttttaatggg ttatggtttg aaagaatgga
     5581 actggaaaga aaaggtccgc tataagcttc ctcaagtatt tatcagcttg attgttttat
     5641 tcggtttgat tattttgatg acacaaattt cactcatcat cagaggaaat acagatacag
     5701 cgctgaggtt ttctaaacca tattctctct tcgcaatgta ttatactttc tcttcgtttt
     5761 tatttttaga aatgctatct gataaattag gtataaaagg aagaggaaat tttcttgctg
     5821 tatttttgat tgtcactcaa gttatatgta atttcccttt agtttcttat cgcgtatcta
     5881 catttatgaa acgtgaattt ccaaactcag ggaaggcatg gctgataaat atcgggcagt
     5941 tttttatttg atatttatta gcagtgtccc tcctagttat ctttttattt tatatacaaa
     6001 aaatccgttt cattcatcgt ttcatcgaat acttcgcttt tgattcgatt gaacaattaa
     6061 ctcagcgttg gaaaaaaaga attcattggc tttttgttca taaaagttat tttttagttg
     6121 cagcattttt ttactgcttt accttcgtgc agattttcgt tttagaacca aaagaaggtt
     6181 ggaaagaaac tatccaggtt gccttaaaaa tatttactca gcgacaagca cccatgatcc
     6241 tgacaatcat cattattatg ggatttttct tcttactatt gttgatttcc aatcgttttt
     6301 ggtacgcctt gactgctacc ttgatcatta acttactgct cactatttcg acagtaatca
     6361 aaatggaaat gcgggaagag cctgtctttc cttcagattt aaaaatgctg accggattgt
     6421 cagaactatt atcgatggtt agcccagtct tattaatcgt gggcggattg atccttctcc
     6481 tattattgat tactagtatt attgtacaac gtagactaca aaaacagtat tccttgaaga
     6541 ttcattggaa acgtcgattt atcagtatcg ctgtattatt aggatgtttt tcgggtgtct
     6601 ttttcattaa tcacaaaaat tcgccttctt tcttgctgtt taatttattt aaagtaaaca
     6661 agactttctt taaccagcaa ggcgccgtaa aagataacgg accagtcatt caattcctaa
     6721 acaatatcga cattaaaatc atggaagaac ctgctggcta ttcgaaagaa aaaattaatg
     6781 gaataatgaa aaagtacaac acaaaggcag atgaaatcaa ccaaacaaga agtgactggt
     6841 taaacaatca gacgatcgtt ctcaatttaa gtgaaagctt cagtgatcct gcacgtgtcc
     6901 ctaatctgac agtacctaca gaccctattc cttacatcaa aagcattata acagaaggaa
     6961 caggaggatt gatgttatcc gtcggttatg gtggcggaac agctaacatg gaatgggaag
     7021 gactgacagg cttgagtatc agtaacctgt ctgcttcgtt agttacacct tatacccagc
     7081 tagtggagcg tcagaaaata agtccaaata ttaccgatct attcgatgaa aaaatagcca
     7141 tccatccttt tacagctgct ctttatcgca gaaaacaagt gtttgataaa tttggttttg
     7201 acaggttcta ttatttagac agtccggata aattaactta tacggataaa atagaacaaa
     7261 gcccacgcat atcggatgat tcagcataca aagaaacctt gaaagcttta caaagcaata
     7321 cggaaacgtc acaattcatc caattatcca ctatgcaaaa ccatatgcct tatgatgatt
     7381 actatagtga attgaactac actgctgaag gaactgctgt cattcctaat agaagaaaag
     7441 aattacaaac ttatatgcaa ggccttcact ataccgatac agcagtaaaa aaattcatcc
     7501 aagaaatcga caaaatcgat aaaccgatta cctttgtttt ttatggtgac catttacccg
     7561 caatctatag tgggaatgat tcgaaaaaat atggattaga acagcaccag acagattatt
     7621 tcatttataa caacaagtat agtcgtgaac aagcagctac gttaaataaa gaagttgttg
     7681 ctcctaataa ctttggtgct atggctcttg agcatgcgaa tatcaaagta actccttact
     7741 atgcgttgtt gacagaagta tctaatcatc tacctgcatt aacaattgat cctacagaaa
     7801 gcttgtctaa tcgttttaat ggaaaacagg tctttgttac agaggatagt caaattatcc
     7861 aagaaaaaaa tttaactgag aaacaaaaag aaattctaga agattatcgt ctgatacaat
     7921 atgatcttgt tgcaggtaac cagtacagtg caacatgggc gacacaaaaa ataaaagact
     7981 aacaaataaa ctgagcaagg ggttgtgata aaagacatgt cacaacccct tgtttatctc
     8041 attaatttgc ttgtccataa acgatttgat taaagccatt tttcaattca ttattcgctg
     8101 tctcgttgaa gtaaagataa ttatcgggat tcccctgatt ttgttgtgtc accaatactt
     8161 ttggctgatt ttgctgaaac ccgaatagat agacgtgctt tttcagatat ggttctgtct
     8221 ctgcatcgga atagacggct gtgatttgat agtctgcttg acctgtccca ctttctgacc
     8281 attctgcaga aacaggcact ccaccaatag ccatcttcca ctccccgttc aaaatagctt
     8341 gagggacttt aagaccatat aagtccacac tcatttgatt cgtataactt ttgtattgtt
     8401 gtcccattgt ttggccccat ttggtcataa atgcctctaa ttgtgcggat ttgtctgcat
     8461 tccacagtgt ttgttcaact tgttcgctgc tttctgaaga ggaagatgaa gcctctacag
     8521 aagaacttga agtagatgtt tgagttgttt cagtcgtttt ggaagactct gacgaagtag
     8581 aggtatctgc tgtttctgta cttgctgcag ttgacttgct ctctgtgcta gttgttgtag
     8641 tagttgcaac tgttgctttc gctgtttctt ttgttgattc ttttttcgat tcattcccgt
     8701 tggtacaacc agctaacaaa aaagctagac caattgttac taaaatcctt tttttcaatt
     8761 aaaaaactcc tttcgattac tcccacttct ttattctatc aaataagcaa tgtgatttta
     8821 ttgatttttc gtattttctc atcaatcttt caaatcttct aaaaactgag aagcacccgc
     8881 tgatgttgtc tgtaatacat cagcgggtgc ttcttttatt ttaatcttcg tgattttcca
     8941 atttacctca cgctttatat ttgtatttga agaaacatag acttttagtc atcatcgtct
     9001 tcctcatcca aatcttgtcg gacagttttt ccttggagtc tttgtacttg gtagatgatt
     9061 tctttattga aacttaaaag ttcaacaagc aattgttgca agccatattt ttcttctttt
     9121 tcagctaatt tgattgcccg tgtgataaag acattttgga aatcaaaatc ttttgcaaag
     9181 ttttccaaca ttgttgaggc tggtaaatat ttgttttgac catgttcgta aatttttcca
     9241 taacgcatga cttcttctaa agtagacgga acgatttcat tttcaatcac taactcatga
     9301 gagattttat tgaaccatct cgcttcttga tcagcatatt ctgaaaaaag ggtacttaat
     9361 tcttgcgaag atgtacccaa aacatacaat tgtgcttgga tcaatttcat ttcttctaat
     9421 ttaaaatttg ctaatacatg ccccaccatg gcgccagcag ttggtttatg atggtcgata
     9481 tctgcttgtt ttgtttcttg aacaaatttt tcttccacta tattcattga tttacgcctc
     9541 ttttactttt gctgattgga cttcataacc taatttttcc acaactgcag ctaattgttc
     9601 agggtggact tcatctggat caaagtttgc tttgattttg ctcgcattaa acaaaacttt
     9661 cgtattgctt accccaggct gttgagaaac cgcatgttcg attttttgca tacagcttgg
     9721 acaagctagt gtatctaatt gcataataat ttttttcatc gtagttcttc cttccttttt
     9781 cttgatagtc ttattctaga ttacagcttc cataaaaaaa ttgattctta tcaagtttta
     9841 cgatttttct tctttgcttt cgtgtccaaa atacaataaa cgcatcgcgt taaagataac
     9901 gatcaagata ctagcttcgt gaacgaacat cccactagcc atgtaaataa acccggcaaa
     9961 taagccgttg agtaaaaata agacgacacc aatggcaatc actacatttt cacgggtatt
    10021 taaaacagtt ttcttagcca agcggtaggc atgaaccaac gattcaaaat tagattgcat
    10081 caagacaacg tcactcgttt cgatagcaac atccgttcct gatcccatag caatcccgat
    10141 atcagccgca gccaaagagg gagaatcatt gattccatct ccaataaaag caacgcgcaa
    10201 tccttgttct tggtattttt tcacaaattg aaccttttcg tctggtaata attcagcatg
    10261 gacttcatca atgcctaaca tatcagctac ataatcagca gtcatctgat tatcacctgt
    10321 cagcatcact aactgttttg ctcctgcttg tttcaactta gctaattgtt cagcgacttc
    10381 tggtcgaatc acatctgaca cacctatgat ttgagtgatt tttttgtcga ttgctacaat
    10441 aatgaccgag ctgcctgttt gttgtaattg atgtaaatct tgtacttgct ttggtgtgag
    10501 agaaagtttg tgagcggcaa tcaatttggt gttgcccgca caaactgtat gaccatcaat
    10561 ctcagctgtg attccttgtc ccttgattgt ctgattatct ttgactgcta attgttgaaa
    10621 atcaatgtct ttctcctgag cataatggat cacggcgcgc cccagaggat gatcggataa
    10681 cgtttctaat cgagccacta aagctaagct gtccccttca tcctctgtat agttcttgat
    10741 agtggataca gcagtagttc ctttggtcaa tgtaccagtt ttatcaaaga caaaagtatc
    10801 gattttggaa aaagtatcca ttgcttcacc gcctttgact aagatgcccc gtttcgctcc
    10861 attaccgatt cctgctacat tcgaaacagg cgcaccaatg actaaagcgc caggacaacc
    10921 aagaaccaaa acagtgattg ctaaacgaaa atcacgacta atcagaccaa caaccaacgc
    10981 aataagtaaa acggttggag tataatactg agaaaatcga tcaatgaatt tttccgcatg
    11041 ggatttggta tcttgtgctt cttctactaa ttcgataatt ttggcaaagg tcgtatcatc
    11101 tccgacctta gttgcttcaa tcgtcacgta gccatctgct aaaatcgttc cagagaagac
    11161 ttcatctcca ctgattttga cgacttgtcg tgattcacca gtcactgcgg cttcatccgc
    11221 ataccctttc ccgtcaatga ctcgtccgtc cacaggaata gctccaccag tttttactaa
    11281 gacgatatcg ccttcttcca cgtcatcgat ttccacttct tcagtcgttc catcttcttg
    11341 gacaacacta gcagtagatg gggccatctc ggtcaaagcc ttgattgact cccgcgtttt
    11401 ggtcaatgtt ttttgttcca gatagctacc aaacaaaaac aagaatgtaa cgatagctga
    11461 ctccttgtat tctccgatca taaaggcacc aagcacagca atagtaacga gtaaatcaat
    11521 tgagataact ttgactttca ctgcctgata cgcatggatc ataataggta tgaccgcaat
    11581 aatagaagct atggctagag caatgttata gaacaagaca aaaccagtaa attttccgat
    11641 aaaaccaaaa ataatcaaca cagctgataa ggcggtgata tgatttcgat gctcttggat
    11701 ccacttttgt aatttcatct ttctccctct tttcctttca ttcttacgag actaagtata
    11761 gatacttatc catcataaaa aattgaccac tatcaagttt agtcaaaaag gcggttttct
    11821 tttggtagaa tacactacaa aaaatagtta gcaagaagac tcactaaaag tgatcttgcc
    11881 aactattgtc gtttctatcg aaattttcat tgaatgattt tcctgtacag catacatcgg
    11941 agatctattt ctttatctaa gatttttatc ttgctaattt tttcctttag tctttcaaat
    12001 ccacgtcgtt catagaattg atacgtacag ccactatctg taaaaaggaa tatttctttt
    12061 cctttctctc ttttttcaaa ttctgttagc aacttggtac cgatgccttt tgttttcaaa
    12121 tcaggatttg cagctaaaaa aatgatctct ccatctggcg aatgcttctt taaatatgct
    12181 gcaagcattt gttcatttgc tgtctcgtaa gctcctttac tttctttagc aaatatattt
    12241 tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa aagaacgatg tttctttttt
    12301 tctcctttta tctccgccag caaaacacct gccaactcgt cctctgcata caaagcaaga
    12361 atttgagttg ctcgattcaa ctccatatac aaaaaatatc ttccgtaaag attcagcaga
    12421 agttcatttt ccatatatct ctcaaagttc attccttcaa cagcaaaata gatagctttt
    12481 ttgtaatctt tcttttctaa gggtttaaaa gttagtttca tcatttcgcc tcctcaactt
    12541 caatgattgt gttatactta cattataata gacacagtgt ctactatcta taatcaatca
    12601 tctataagtt tgtcgattaa gcgacagctt tgctttgata tgtcggataa ggagataggt
    12661 aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa agctttggct gaaacactat
    12721 ccacattatt agtaaataaa aagattcaag caatcactat acgcgaacta acagaaacgg
    12781 cagatgtaca tcgttcgact ttttatacac attataagga tatctatgat ctatatgatc
    12841 aattagaaag caactttttt cgggatctaa atgaaattct ctcttatgat ccagcccata
    12901 gctatgagga actatacact cgcttgattg actatgttta cgcaaatact tttttatatc
    12961 gcttatttat cctcaaccat ggtgatattt cgtttcagaa gaaagttaaa caagttattg
    13021 aagaaaacta tctgaacata tggctatatg aagacaagaa aacgattatc acagaagaaa
    13081 tgagattctt tactacgtac cacgtccaag gttgtctatc cgttctcaat cggtgggtag
    13141 aaagcaatta caagtactcc aaaaaagaat tattagaact cttacagaaa ctgaatgatc
    13201 acatagagaa aatcatgcct tgaataaaaa catactaagc ttagttaaga agaaccgtaa
    13261 ccttctcgta taacaactga atcttattta gccaaattgt ttactgaaga ttttttgagt
    13321 aaaatttcta taattcctac ttattgaaaa gtagtttgta gtgctgcttc tccctctcta
    13381 agtgatatat cttgttgtac caatcaaatt cacctcatca ttaataaatt acttttaggc
    13441 taatttatta caacttatag gttgaagctg taaccaattt atagtcatat tatattttta
    13501 tcatccagtt tattgaattc ttctcgttta cctcaattca ttttcaaggg gaatca
//
LOCUS       DAYYGY010000076        13353 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000076 DAYYGY010000000
VERSION     DAYYGY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.077"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..891)
                     /locus_tag="ACHA52_002262"
     CDS             complement(166..891)
                     /locus_tag="ACHA52_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940935.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1045..1371)
                     /locus_tag="ACHA52_002263"
     CDS             complement(1045..1371)
                     /locus_tag="ACHA52_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940936.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1408..1773)
                     /locus_tag="ACHA52_002264"
     CDS             complement(1408..1773)
                     /locus_tag="ACHA52_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8940937.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1810..2025)
                     /locus_tag="ACHA52_002265"
     CDS             complement(1810..2025)
                     /locus_tag="ACHA52_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8940938.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2154..2489
                     /locus_tag="ACHA52_002266"
     CDS             2154..2489
                     /locus_tag="ACHA52_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8940939.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2565..4562)
                     /locus_tag="ACHA52_002267"
     CDS             complement(2565..4562)
                     /locus_tag="ACHA52_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940940.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4660..4935)
                     /locus_tag="ACHA52_002268"
     CDS             complement(4660..4935)
                     /locus_tag="ACHA52_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940941.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5167..5901
                     /locus_tag="ACHA52_002269"
     CDS             5167..5901
                     /locus_tag="ACHA52_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8940942.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5940..7082
                     /locus_tag="ACHA52_002270"
     CDS             5940..7082
                     /locus_tag="ACHA52_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8940943.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7710..8195)
                     /locus_tag="ACHA52_002271"
     CDS             complement(7710..8195)
                     /locus_tag="ACHA52_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8940944.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(8197..8868)
                     /locus_tag="ACHA52_002272"
     CDS             complement(8197..8868)
                     /locus_tag="ACHA52_002272"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940945.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(9131..10324)
                     /locus_tag="ACHA52_002273"
     CDS             complement(9131..10324)
                     /locus_tag="ACHA52_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8940946.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(10408..10608)
                     /locus_tag="ACHA52_002274"
     CDS             complement(10408..10608)
                     /locus_tag="ACHA52_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8940947.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(11032..11265)
                     /locus_tag="ACHA52_002275"
     CDS             complement(11032..11265)
                     /locus_tag="ACHA52_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8940948.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(11272..11703)
                     /locus_tag="ACHA52_002276"
     CDS             complement(11272..11703)
                     /locus_tag="ACHA52_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8940949.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            12012..13351
                     /locus_tag="ACHA52_002277"
     CDS             join(12012..12453,12453..13351)
                     /locus_tag="ACHA52_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8940950.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         4235 a         2635 c         2183 g         4300 t
ORIGIN      
        1 tttaatgaaa ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc
       61 cgaaaaaaaa tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat
      121 ataacaacgg ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt
      181 tttaaatact tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt
      241 tttcccattt tcaagtaacc gaagtggaat atctttacca actttctctg tcactgcatt
      301 actgatacct gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc
      361 tgtatcagta tttatcgtga ttcctttatc ttcatttaca agtttccaag taggtggtat
      421 agctgtgtat gtttctacaa atgaattaag tttttcatta agtgacttct cttttatatt
      481 tccatctaaa aactctgtat atatggcttg agctatacta aaacaaagct ggccttgttc
      541 taataatttt acatcattag ttagtaaagc tgtgttcacg ttatcaatcg cttcaagaag
      601 agtatctttg gtgtccctaa cagctttagg tgtgccttct ggaatttccc tacttgatac
      661 ctcgtccatt ttatcttcca taaacccctt aaaatcatat ctttctccca aatccataga
      721 agtttttgag aaagctttca tttctgagtc tatttcttca ataatattta acatgtctga
      781 tacatattgt ttaggctgat tcgtttttaa tctatatgtg gtaaatatct caattcctcc
      841 aattatcaat aaaattgcca aacaaatcca taataatttt ttctttttca tttttcctcc
      901 tatacactgc tgttataaca gtgactaatg taaagatttt cccagcaatc atcagactat
      961 tatttacagg gaatcaaaaa aactccttag taagcttaca cctttaggag ttcctgtttg
     1021 aacggtatgc ataatacatt taaatcaatt taacattaac acctcagtat atggtatctc
     1081 tacatataca cggtcttttt ttggatagta ctcgacattt atattgatag cacttccatt
     1141 gtctaacgta gctttagccc ggtacgtgtt atcaccatta tcatgagtga tcgtcacatc
     1201 atcacatgtc cgatttaagt tatactgttc ttgtaaaatc tgagttacca atggtacaga
     1261 tgacttctct agtccagatg aacccgtcat tcttgaaaaa attataccta taatagccaa
     1321 tacaacgata cctataatga caaacttctt attcatattt actttattca actttttatc
     1381 tcctatctcc aaaatgattt tctctcatca gtaactcatt gacatcccca atagtacacc
     1441 aaagatggct ccagctaccc ctgctatcat caaaatcgta cccatttgtt catctttctc
     1501 tcgatataag taacccataa taacagcaac agcaccaaat accacaggaa taaatgctaa
     1561 tgatactacc atagaaatcc aaccaataat caaatacagc ttccctgtct cttgtccatg
     1621 tacgatttgt tccttcattt cattaattgt ctgctcagta cttttcttta ctgtgtctgt
     1681 tccggttgtc tcatctgttt cttgtaatgc tccgcaaaac tggcaatact tactttcatt
     1741 tgttatttct ttaccacatt ttctacaata catgtcttct cctcctaaaa tcattcaatt
     1801 gcttattagc taactgttta ctttattaaa tacatcagaa aacagctcat ttattggtac
     1861 acttaatatc tcagaaagca ttttcatttg atagggttta ggaatcgttc tccctgtttg
     1921 ccacgaactt acacattgct gtgtaacatt catttttttg gctaactcag tttgggaaag
     1981 tcctttttct tttctgtatc ggctgagatt acttactttt ttcatgattc tcccctcctg
     2041 ttttattata tacaacaata tgatgtattt caactcataa aatatataaa tacttcaaat
     2101 gtcattacaa atacaacaac ttgttgtact ctactgagaa ggggggtgta ttaatgaaac
     2161 agaagcaata tgcaaaggtc attagagaaa agcgaaaagc actcgggtgg acacaaaaag
     2221 aattagctaa aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa
     2281 cagaacctaa cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc
     2341 attttctaga caatgttgac atagattatg aggaagagtt tttggctctc tatcgctctc
     2401 ttagtactga agatgcaatt cgaacaattg actatatgaa gctattaaaa aggcaagaaa
     2461 atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg
     2521 ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt taatataatt
     2581 gggtctcttc tattccttac tctccgtcca tcatctcttg ttacacttgt aaattgataa
     2641 ataggaacta attcaactcc tgataatatc aattctttta ttacttcatc tatccctgcc
     2701 cctttcgcta gatatactaa aaaactactg cttgctttca cattgtatag tttcttaata
     2761 tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttctatt
     2821 aaagcatttt tcacgctgtt atccctgaca tttcgtaggc gttcacaaac agtgttaata
     2881 agttcttttt ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt
     2941 gcttcatcta aaatcaattg tttttccttg ctaagttctg atcgggaatt ttgaggaaat
     3001 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
     3061 tatgtaaaat gaaatttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca
     3121 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgttt aaagtttcct
     3181 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
     3241 tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta
     3301 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac
     3361 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca
     3421 tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc
     3481 attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
     3541 ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt
     3601 aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa
     3661 taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg
     3721 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
     3781 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
     3841 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg
     3901 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
     3961 tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct
     4021 gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg
     4081 atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct
     4141 tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt
     4201 atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctat gcttaagtat
     4261 ctactatcat ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg
     4321 acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt
     4381 gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta
     4441 acttctctat agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact
     4501 ggcttctcta ataatttttc tttatctaca taaattggaa tattaatcat tgatgtttcc
     4561 atttgtgttt cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca
     4621 aactctttat tactatatgt tttagtctta tttgtttact caacctttat accgttccat
     4681 gaaactatca atatcagatt ttttaaatct aatgaccgta tcaattttta taacaggtaa
     4741 cccgtattga gaaacatatt ttctaatcgt tcctactgat acacctagat attcacttgc
     4801 ctgctgatag tccatatact ctagagtgac tccagaaata atgtcttcta gcataggctt
     4861 tagcactgta attatctctt tcacttgttg ttcaacttgt atagaaacaa tgctatcaat
     4921 gatttgtaga gacatatttc cccctccttt cgtgattgct tgaacaaatt ttctaagaat
     4981 tatcttcgta ttttttgctc aaaataaaag tattttgtta ttaaccgatt aatcaaacaa
     5041 aaaatgatta ccagttcatc gacatacaca taatacctcg ataaaaaagg ggtgtcaata
     5101 gttttgtaaa atttatttac aacacccctt ttttgaggta tactaatttt aggaggtgtg
     5161 gtcaatatga taaaacgtcc accaataaat tatttagaac gaaaaaagat actcggaaca
     5221 aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt
     5281 aacaacggtc aattaattga caaaaagact atctacgaat gggagaaggg aacctattta
     5341 ccaataccag agcgattatc ccgaattgct gatttgggaa atatgagtat agaggagtta
     5401 gtttgtggca atgtagaaga atatattttg ggaattattc tttatagaga tagcattgta
     5461 ctagatggta taacatttcc agataaaaac ctgttccagc atcttcgtca acagttccca
     5521 ccagtacact ctaatttaga tacatggctg gatagatatt ccaaattaga accagagatg
     5581 caagaattta ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc
     5641 aatatcttaa aaatagaaga attattcatc aatgctattg tagaagaatt tgacaataac
     5701 attcttttcc tcactagcag tatagaagaa ctactagaaa gaatggtaga tgaatggcta
     5761 cctattcaat tgaaagacat gtcttatccc gaagaagctg tgagagaaat tactgataat
     5821 ataaacaagc tagaacaaac gatttcatca attgggaaaa aatacactaa gaaaaaaatg
     5881 aagggaggtg ataccatcta gtggtaagta ctccttgtaa actaagagga gatgatttca
     5941 tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac
     6001 acattgccac cgatccacga actggtgaaa aaataaatac tacgagaaga ggttttagta
     6061 gcaaacaaga agctcaaaaa gattatgacg agtacagaca tcaaatatta tacggtgtta
     6121 aaaagaaagc acctgatatg acatttgaag acctgtataa tgaatggata actcatcagc
     6181 gtaccagtgt taaagcttca acaatcgcaa tctcagtaag atacgcaaat aatcaaatac
     6241 ttccagcatt tggtaagctt aaactatcga atatctctgt cccatattgt caaaaagtgg
     6301 tagatgaatg gcacagtaaa tatgaaagtt acgattatat gagaaaacag actgcacaaa
     6361 ttttacgcta cggggttgca atgcaatata ttgacaataa tcctatggaa aaaacacttc
     6421 ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag gagcttaata
     6481 atttactgga tgcttttaaa gattttggta acatgaaaca atacgccttc ttcagattat
     6541 tggcatacac aggtatgaga aagagtgaag tactcgcttt acagtggaaa gacatagaca
     6601 cgtttaataa agaactacat gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta
     6661 taattcaatc tccgaaaaca agagcttcta gacgagttat ctctttagat actgagactc
     6721 tttctatcct caacaactgg aaattacagc aaaaagaaga gtatttaaaa cttggataca
     6781 atactagctc taaagagcaa catgttttta caacagttaa aaacactcta tacattccaa
     6841 acactgttaa tgattggtta agatatattt tgaaaaaata taacttgcct cgtattacac
     6901 ctcatgggtt tagacataca catgctagtt tattattaga agcaggagaa tctgtcaagg
     6961 tagttcaaca acgattaggg catgaaaata gtaaagtgac attagacatc tatgcacata
     7021 ttacaaataa tgcacctaag aaaacaggtc aagactttgc tgatatgatg gctcatcagt
     7081 aaaagagctg gtcaaaatct ggtcatttct agtatacagg caaaacaaaa aagctagaac
     7141 ccttatatat caagggttct agctcctata acaggtttac ccaacactgc cttccaatga
     7201 ctcttttggg aatcagagga aagaatcagg ctttatagag atatttcatc taacaaatta
     7261 ctttagactg acagtatata tcagcaggta ctgtcatcgg gaacgtagcc aatatcgtat
     7321 ccaaaacagc aggtagagaa gctatttgag gattcaagtt attctatttc aaccatgaag
     7381 ttaagttcat taaaaataaa gatagttggt atttcaataa tttttgtata gagttaatca
     7441 ctttattgat gctaattcat cttgattggt attataatcg tattcaagtt gcgtggcgtg
     7501 cttttgctag aaaatatata actgttcttt ataaagaaat ataaggatta aacatttttt
     7561 atcatagagt tttacttatc tatagaatta tgtctgttcc taattcagtt tgatgaatta
     7621 actgataaaa aaatatctac tatttaaaga tacagggata agtaagtaaa aaaataacat
     7681 gtcattagat agtagacagt tccaactctt tatttacctt ttttgtatgc tgcactgtca
     7741 atccaagttc ctaaattgtt gtatccatca tcacttatat aatcatattg gccatcaata
     7801 atttcgtcaa accacaaata ttctccatct ggtttttgcc caattatttt aattactgat
     7861 tgactagtag ttgtcatctg gttaaaatct tttattttac ctatattaac ggccaaaata
     7921 gcatttcctc gcttataact ctcctggatt tcatatttaa catattctcg atcaagagtt
     7981 tctttaccta tcagcaccac agtcactgat gttccaatta attgattatc tatccagttt
     8041 tttatagcaa tatctccatt acgtttaact ttctcaaatt cagccttatc tacaaaacca
     8101 gcttcctttg ttcctcgcaa agtccagctg tttctaacta ccattgctct tgttacatct
     8161 gcataatgga aactaaaaaa aacccttctc accatattaa tcctcctgaa tactatttat
     8221 taaactgaca atttgttcaa cttttttatc tacttctcct tcagtatcta atttttctag
     8281 cgcttgaaat ttacttttta aggaatcatc aaaataatca ctgaaattct cttgaatctt
     8341 atcaaacaaa accttacttg cacctttggt aaatccaatc ggaactacta tattttcatt
     8401 ttgaacagat aattcaaatt cttcaatcat tccatctgct gtaactattt cacctgattc
     8461 aagtttgttt ccaaaaacaa aaatactaat cccagactct gcaataatgt tttctcgata
     8521 tcttctccac tcatgaggca tatcaagggg aagtggtctt aaagaaagta cgtcttgtat
     8581 gttatttttt cttaaatttc ttctctgctt tagaactcct gaaacaatac tattaccaat
     8641 tccaagtccg taacctgata ctattctata accggattca gaaagcctat atgctaattt
     8701 ctcgacaaat tttaatcctt tcgattcaga tgtaaatgga gtataatcaa cagcacttcc
     8761 cgaaataaaa atagtttttc tcaaatactt ttttctaata cttttcaaaa tatcagtaat
     8821 ctcagaataa tcatctactt caacaacgtt tatcgaaaat ctggacaaat cattgatttg
     8881 tagtcttctt tttgtcagtt cataattata tctctcttga tcaaaaaaat cctcagaatc
     8941 gacaaaattt tcctttaaaa ttatatggga ttttagatta tctttttaga agttcttgaa
     9001 tgtcggtata gcccgtgttt ccgggctttt ccggcatttt tgatattgtt agatacttac
     9061 aaatgttctt attacctctc accatttccc cctcaaaagt agtaaatgga gtagtaaaac
     9121 cggccttgtg tcaggctgcc agccgttcca tttctgccct tgcggaatcg aatgtggcgt
     9181 gtgcataata gttcagcgtc atggtgatat tggaatgtcc cataatgtac tgcaaggctt
     9241 tggggttcat tcccgcattg gctaaccggg tgcagaatgt atgacgcata gcatggggcg
     9301 tcgtcgtttt cggcaacgct tcttcatggc tcttgttgta cttcttcaca agcctgccaa
     9361 acatcccgcc ataatctacc gccgtcatgg gatagccgtt ctgtttgaga aacaggaaat
     9421 tggcataccc gtcaataatg aacggctttg cacctttgcg gttttttatc actcgctgga
     9481 acgcctgata cacttcctca ttcatgggga tttttctgac gccgctttgg gtcttgggtt
     9541 cgtcaatata gtaaccgacg ccggtatttc tcaaaagctg gtggctcacg atgattaccc
     9601 ggttttcaaa atccaaatct ttgtccgtca gtccgcacag ttccgaaatg cgaagccctg
     9661 tccccagcag aataacgatt gcgtcataat gcttttggta aaccttatca ccttgaataa
     9721 aggacagaaa actttcttcc tgtgctggtg taagaggcac ctttggttcc gtatcgtcgt
     9781 ccagaacgtc actaagctgg aagtcaaacg ggttttttcg gatgcaatcg tcctgtatgg
     9841 cggtgtaaaa agccgctttc agggaacgct tgtcgttatt gatggtctta taagaaaggc
     9901 ccttttcttt catccgaagc gcccattcct ttgcgtcgga cagcttcaca ctgtcaatcg
     9961 ggcagctccc cagccgatct tgttccagaa tacgcatcag ccgttcccgg cccagtttgg
    10021 tgttgtgctt cacattgccc cggtgcctga tctgcttggc gtagagctgg cagacggtca
    10081 ttttcttgcc gatggtgtct atcccgtcgt caaggtcttt ctggatttct ttctcctttt
    10141 cccggagtga aatatcctcc cgtttacctg ccggggtctt atccgtaggc actaacttcc
    10201 atgagtaaac aaactgcggt tttccaaagg tatcaacata tttgtaagca tatcgtccgt
    10261 cttttctctg gctctctcca gaccgcaaaa cgcgattttt gctgtctcgt cttttttctg
    10321 acatctcgtc atgctccttt ccatgacgga aagagccgca tatatgctta taaattatta
    10381 taacatatct gcggcccttt ttcactatta gattgtgtcc aaagtgtcaa ttattttctc
    10441 aaactgcttg cgtttgatct ggatgcggtt gccattcata atgacccagc cagaggccgg
    10501 attttcctcg gccagcttac gcaatttgtt ttctccgata cggaaatatt tagacgcttc
    10561 ctcaatcgtc agcgtgtatt tttcccaaat aggcacatca tggttcataa tgcagccccc
    10621 tttcacaaat tcaaaggttt gtgtgattta ggggcataga cggacggctg ctggcacagc
    10681 tccacgggac tttcaccccc gcgtggttct cacgcggccc tacccattgc ctgcgacgct
    10741 tttaacgctc ggactgtggc tataagggag tatcatagtc ctgcctgtac gtcgtcgccc
    10801 gcaagccgcc acgctcacat tgcggcgtgg tgttcctcgc tccgcttttc ccgtagggaa
    10861 aagtatcatg gcgcaccagc cgtccggctc ggcacagaca gaatgtgtcc gtatgcccta
    10921 tgatgcgccg ccgttatctt gcgggcatat ttatcaaggt gcgatcaggc caccgggaaa
    10981 acaccgaaac ggggaaaggg taagccgttt catgctgccg gggccggttc gtcagttcgt
    11041 gacgatagcg tggatgagct tgatttccag acggcgcttc atggcctcgt ccagacacac
    11101 atgcggatag ccgttttcgt catagagcgt ccgggtacac agcttgttca tgtaaccgct
    11161 gtaatactgc aaaacgcgct ctacggctcc ggcatcccca gcgcgggcgg cctcgatcac
    11221 cgaaaggggc aaaagctcaa tgcccttgta gttcgggttc ttcatccgcg tctaacctcc
    11281 cttcggcatc aacgccttta gctgtttccg cagctccgat aaggtatttg tcctgtgccg
    11341 ctgaacggca ctccgggaca ttcccacaag gccgccgatc tcaccatctg ccatatccag
    11401 aacacagtgc aggattaaaa tactctgctc catgctgggc agcgcggcaa aggcttcggc
    11461 aacaagctca ttgttgatat gtaggtcata gccatgcgac gaaaacgtaa agctgtcgct
    11521 ggggtagtga tctactgtac agagcttgtc caaatctgcc tgtgacaaag agcttagtga
    11581 cacttcacgg tcacggcggc gcttcatttc agccaagtag cttttggcct cgttacgcag
    11641 taccgtcttg caaaaagcat cgaacatgca ccgcgcttca tagtcgcggg ggatagcatc
    11701 catcttctca ccccctttct gtgggggact taggtggttc tctccctttc cgctaacaag
    11761 actttgacaa ggccgccagc tacccgctaa atacctcctt tttgaaaatt tctcaaaaaa
    11821 tttttttggg gacagccggg ccgcaaaaga cgacaaaaat cgcccggcag gtcgcaaacc
    11881 taccgggcga gaaaatgctt tgctatgctg ctgatttaca tggtatagta catactcata
    11941 accggaatag ccctgtggtg atgtgcaccc caaaagttag actagaaatc taattaaaag
    12001 gggtgctttt tttgcggaaa tatacatttg tttttaagaa aaaagtagtt tcagactatt
    12061 taaataacga aggcggctac aaatatcttg cacataaata tcaaataaat cgtacactgg
    12121 ttagacattg ggtaaggatt tataactatc atggttggga aggcttggtt ggaggtggca
    12181 aaagctacac tacaaaattt aaacttgatg ttatagaata tatggaaaca aatggtcttt
    12241 ctatccaaga aactgctaaa aaatttaata tcggttcaaa tagaactcta agtaaatgga
    12301 tagagcaata tgaagaaggc ggtgcttctt cacttgagag ccaaaaaagg ggcagaaaaa
    12361 ttagtatgaa ttccaagcta aacattccta aaaaacttaa agatgagtct cttgaagaag
    12421 aagttattcg tttaagagca gagaacgcat atttaaaaaa gttagaaacc ttgattcaag
    12481 aacaagattt gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt
    12541 taaattgaat ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt
    12601 aaaaaaatta gataagccag ataaatatag caagataaag caagaaatta cagcgattgt
    12661 aaaagaatca agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg
    12721 atatacaatc aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca
    12781 aatccgaata aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt
    12841 gttaaaacga aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga
    12901 attcaagata aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga
    12961 aataattagt tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca
    13021 acaagcaatt aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg
    13081 gcagtatcag atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat
    13141 gtctagaaaa ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa
    13201 atcagagttt ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa
    13261 tgagtatatt aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc
    13321 agtagaatat cggaaacagt ccattaaata gtt
//
LOCUS       DAYYGY010000077        13163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000077 DAYYGY010000000
VERSION     DAYYGY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.078"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1851
                     /gene="yfcC"
                     /locus_tag="ACHA52_002278"
     CDS             112..1851
                     /gene="yfcC"
                     /locus_tag="ACHA52_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8940951.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2008..3192
                     /gene="iadA"
                     /locus_tag="ACHA52_002279"
     CDS             2008..3192
                     /gene="iadA"
                     /locus_tag="ACHA52_002279"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8940952.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3303..3506
                     /locus_tag="ACHA52_002280"
     CDS             3303..3506
                     /locus_tag="ACHA52_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8940953.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3607..4269)
                     /locus_tag="ACHA52_002281"
     CDS             complement(3607..4269)
                     /locus_tag="ACHA52_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940954.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4282..5784)
                     /locus_tag="ACHA52_002282"
     CDS             complement(4282..5784)
                     /locus_tag="ACHA52_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940955.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6332..8011
                     /locus_tag="ACHA52_002283"
     CDS             6332..8011
                     /locus_tag="ACHA52_002283"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8940956.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8138..8617
                     /locus_tag="ACHA52_002284"
     CDS             8138..8617
                     /locus_tag="ACHA52_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8940957.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8836..9516
                     /locus_tag="ACHA52_002285"
     CDS             8836..9516
                     /locus_tag="ACHA52_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8940958.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9524..10855
                     /locus_tag="ACHA52_002286"
     CDS             9524..10855
                     /locus_tag="ACHA52_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8940959.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10870..11832
                     /locus_tag="ACHA52_002287"
     CDS             10870..11832
                     /locus_tag="ACHA52_002287"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8940960.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11943..12281
                     /locus_tag="ACHA52_002288"
     CDS             11943..12281
                     /locus_tag="ACHA52_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8940961.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12360..13067
                     /locus_tag="ACHA52_002289"
     CDS             12360..13067
                     /locus_tag="ACHA52_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8940962.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4184 a         2321 c         2888 g         3770 t
ORIGIN      
        1 tgattttcta ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt
       61 atccattttt tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa
      121 acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat
      181 gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga
      241 aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact
      301 gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat
      361 caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa
      421 ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa
      481 atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct
      541 aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc
      601 ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc
      661 gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc
      721 gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg
      781 ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt
      841 ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca
      901 gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt
      961 agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga
     1021 ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa
     1081 aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct
     1141 catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta
     1201 ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac
     1261 attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt
     1321 cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca
     1381 gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg
     1441 ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg
     1501 gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg
     1561 ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct
     1621 ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt
     1681 tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt
     1741 gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt
     1801 gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga
     1861 cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg
     1921 gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag
     1981 ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca
     2041 ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa
     2101 gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa
     2161 gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc
     2221 ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg
     2281 acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc
     2341 aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga
     2401 ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt
     2461 atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag
     2521 ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatcgggaaa agctgggatc
     2581 atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa
     2641 gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta
     2701 tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa
     2761 gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga
     2821 ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc
     2881 ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca
     2941 ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta
     3001 cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa
     3061 attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt
     3121 atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa
     3181 aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct
     3241 gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca
     3301 caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa
     3361 aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat
     3421 cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt
     3481 tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa
     3541 aaaatccaaa ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc
     3601 tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca
     3661 gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc
     3721 gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg
     3781 ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg
     3841 ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac
     3901 acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc
     3961 cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa
     4021 aataatcgag acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc
     4081 ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt
     4141 cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag
     4201 ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt
     4261 ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat
     4321 ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag
     4381 tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa
     4441 tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc
     4501 tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc
     4561 aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa
     4621 tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga
     4681 ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat
     4741 gatcagagta agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa
     4801 acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa
     4861 gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc
     4921 tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag
     4981 ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt
     5041 catacccata ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata
     5101 agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt
     5161 tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat
     5221 tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc
     5281 tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc
     5341 agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt
     5401 gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt
     5461 tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa
     5521 actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt
     5581 tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc
     5641 ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag
     5701 aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt
     5761 ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca tttgataata
     5821 gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt
     5881 tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta
     5941 tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac
     6001 agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat
     6061 ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt
     6121 cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca
     6181 gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt
     6241 tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta
     6301 aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg
     6361 cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat
     6421 tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc
     6481 tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg
     6541 acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt
     6601 cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa
     6661 aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt
     6721 agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac
     6781 aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat
     6841 gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact
     6901 aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga
     6961 tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag
     7021 taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt
     7081 cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca
     7141 attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga
     7201 actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc
     7261 aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac
     7321 gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg
     7381 tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga
     7441 cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta
     7501 tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat
     7561 ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg
     7621 aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat
     7681 tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat
     7741 cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta
     7801 cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga
     7861 gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca
     7921 attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat
     7981 ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata
     8041 gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc
     8101 gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga
     8161 acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga
     8221 agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac
     8281 tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca
     8341 cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat
     8401 ggattttgta ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa
     8461 actcgcacat cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt
     8521 aattgtgtca agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa
     8581 gatcgtccat cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca
     8641 gagcagagac ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc
     8701 tgttacaatt gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta
     8761 tattttttca ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa
     8821 cgcgaggtgt aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg
     8881 gaaaatggcc aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta
     8941 actgcagaag aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata
     9001 ggagatatga ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt
     9061 gctgattatg cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat
     9121 gcgtttccat tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta
     9181 gacggtatcg ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa
     9241 acgatttatc atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa
     9301 ttcgatattg atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat
     9361 gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact
     9421 ttccctgtca ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga
     9481 aaaataatga gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga
     9541 tttgatttta gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat
     9601 cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa
     9661 tgtcggacta gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa
     9721 aaaacatatt gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt
     9781 aggcggggcg gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa
     9841 acatgccatg gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga
     9901 agtccaaaaa ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac
     9961 gactgctgag attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta
    10021 tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa
    10081 agatatcatg cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca
    10141 gacagatacg atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc
    10201 agtcgtcaac cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa
    10261 atcgccaaac cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac
    10321 aatgagtatt gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt
    10381 cgtttccgat gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat
    10441 gcagcttgtc cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa
    10501 tatccatacg attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt
    10561 cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga
    10621 aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat
    10681 tgaacaagtg aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg
    10741 tccacgtgta ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga
    10801 aaacaccatc gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc
    10861 aggagtgaaa tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc
    10921 attatccaca ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc
    10981 gaactattac gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa
    11041 gggcttcgct ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta
    11101 gtcatcgtga ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca
    11161 gaaaaactga tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg
    11221 gtaaatacaa aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct
    11281 gaatctttaa agataaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat
    11341 acagggcgct ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg
    11401 agaaattatc catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa
    11461 gtcgctaaac tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga
    11521 agaataatcc ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct
    11581 tcgatcgttt cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag
    11641 cagccttcag ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg
    11701 gctaaacgcc atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta
    11761 gcagaagtag aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga
    11821 aaaacagaat gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc
    11881 ttttcaagat cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga
    11941 agatgacatt accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct
    12001 tgttgatttg cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag
    12061 aagacggatt agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga
    12121 cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag
    12181 tcaagaacat ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg
    12241 gaccgatgaa actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt
    12301 cacattcttt tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa
    12361 tgtatggtta tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat
    12421 tagttatttc cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag
    12481 tgagaagcaa aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac
    12541 agataaataa tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag
    12601 gtaataagat gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag
    12661 gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga
    12721 agattcgtgc agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga
    12781 ttatggtagg tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt
    12841 tttctttagc attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc
    12901 gagcaatcca aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa
    12961 gacacgtatt atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc
    13021 agctgttgcg cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg
    13081 tgaaatcagc gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt
    13141 tggaataaga ggatgtgaca aga
//
LOCUS       DAYYGY010000078        13084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000078 DAYYGY010000000
VERSION     DAYYGY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.079"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACHA52_002290"
     CDS             complement(78..2198)
                     /locus_tag="ACHA52_002290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8940963.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACHA52_002291"
     CDS             2428..3606
                     /locus_tag="ACHA52_002291"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8940964.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACHA52_002292"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACHA52_002292"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8940965.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHA52_002293"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHA52_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8940966.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACHA52_002294"
     CDS             complement(4959..6212)
                     /locus_tag="ACHA52_002294"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8940967.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHA52_002295"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHA52_002295"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8940968.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACHA52_002296"
     CDS             7789..9135
                     /locus_tag="ACHA52_002296"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8940969.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACHA52_002297"
     CDS             complement(9443..9829)
                     /locus_tag="ACHA52_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8940970.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACHA52_002298"
     CDS             complement(10097..10246)
                     /locus_tag="ACHA52_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940971.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="ACHA52_002299"
     CDS             10708..10920
                     /locus_tag="ACHA52_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940972.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="ACHA52_002300"
     CDS             complement(11198..12997)
                     /locus_tag="ACHA52_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8940973.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DAYYGY010000079        13071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000079 DAYYGY010000000
VERSION     DAYYGY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.080"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..848)
                     /locus_tag="ACHA52_002301"
     CDS             complement(<1..848)
                     /locus_tag="ACHA52_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8940974.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLT"
     gene            complement(845..1498)
                     /locus_tag="ACHA52_002302"
     CDS             complement(845..1498)
                     /locus_tag="ACHA52_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8940975.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1786..2306
                     /locus_tag="ACHA52_002303"
                     /pseudo
     CDS             1786..2306
                     /locus_tag="ACHA52_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2304..3023)
                     /locus_tag="ACHA52_002304"
                     /pseudo
     CDS             complement(2304..3023)
                     /locus_tag="ACHA52_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2974..3183)
                     /locus_tag="ACHA52_002305"
     CDS             complement(2974..3183)
                     /locus_tag="ACHA52_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8940976.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(3165..3233)
                     /locus_tag="ACHA52_002306"
                     /pseudo
     CDS             complement(3165..3233)
                     /locus_tag="ACHA52_002306"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3460..4733
                     /locus_tag="ACHA52_002307"
                     /pseudo
     CDS             3460..4733
                     /locus_tag="ACHA52_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4840..5871)
                     /locus_tag="ACHA52_002308"
     CDS             complement(4840..5871)
                     /locus_tag="ACHA52_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8940977.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5878..6708)
                     /locus_tag="ACHA52_002309"
     CDS             complement(5878..6708)
                     /locus_tag="ACHA52_002309"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940978.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6705..7520)
                     /locus_tag="ACHA52_002310"
     CDS             complement(6705..7520)
                     /locus_tag="ACHA52_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8940979.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7517..8341)
                     /locus_tag="ACHA52_002311"
     CDS             complement(7517..8341)
                     /locus_tag="ACHA52_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM8940980.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8331..9431)
                     /locus_tag="ACHA52_002312"
     CDS             complement(8331..9431)
                     /locus_tag="ACHA52_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8940981.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9609..10394
                     /locus_tag="ACHA52_002313"
     CDS             9609..10394
                     /locus_tag="ACHA52_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8940982.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10427..10810
                     /locus_tag="ACHA52_002314"
     CDS             10427..10810
                     /locus_tag="ACHA52_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8940983.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10886..11017
                     /locus_tag="ACHA52_002315"
     CDS             10886..11017
                     /locus_tag="ACHA52_002315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8940984.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10986..11222
                     /locus_tag="ACHA52_002316"
     CDS             10986..11222
                     /locus_tag="ACHA52_002316"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8940985.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11234..11320
                     /locus_tag="ACHA52_002317"
                     /pseudo
     CDS             11234..11320
                     /locus_tag="ACHA52_002317"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11300..12271)
                     /locus_tag="ACHA52_002318"
     CDS             complement(11300..12271)
                     /locus_tag="ACHA52_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8940986.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3945 a         2880 c         2322 g         3924 t
ORIGIN      
        1 tttgttaaac tatcaacgat ttcaatacat tcattaatat aaatatctct ttctttatat
       61 tttccaatat tgtatttcat gttttccagt attattttaa gactagcaag aggggtttta
      121 agttcatggg atgcaccttt aaaaaagtca tattttaact tttctaattt taaaatttct
      181 ttatttttaa attccaaatc gtcaattgtt cttaataaag tagaatacaa atcattaatt
      241 tgttgtttta attctccaac ttcatcatta gagctaactt ttaaactcat ttttttatca
      301 agtttcatca ttttatcagt aacaattttt atttcttgta tattattttt tattgatttt
      361 gcataaatta aagaaataat agcagaaaat aaaattgata ttaataaaga atatggtaaa
      421 aatttaaggc ttaaatcttt cgcatctttt tgcatatcag ctgtagaaac aaattgtaga
      481 tttgtttttt taccatcatt aagctttatt tctctttctt caattatcaa agaattacta
      541 tcactttcta aattaacatt tatattatct ttgatttgta tttcattctt attatttttt
      601 tcttttatga atacttttat ttcactactc ttggaataaa actccaaagt ttgttctata
      661 tattttattt cttttccatt catattacta gaaatttcat ttgccatatt atgaatcttt
      721 tcttttctcg tctccaaata tgttttagga aagataaaaa aaattaatga atgaactaat
      781 ataattatta ttccaagaac agaaaatatt tgtatgaaca tttttggaaa tatctttaat
      841 tttttcattt tctctccaat ttatatccta catttcttat agtagttata cagtctaatt
      901 gtaatttttt tctaagttct tttatatata catctattac tcgatcataa ggagtttctt
      961 cgctatcttt ccatacataa tcaataattt gcattcttgt taacacttgt ccattattat
     1021 ccaataaaca ttttaatact tctagttctt ttgcatttac atctatttct tcaccattta
     1081 ttttagctgt atagctatta aaattaaccg atagatcctt atattcaaac ttctctaaat
     1141 gtccataatt cttcttaatt aaagaatcta ttctagcttt taagaccggc aatgaaaatg
     1201 gcttttctac atatccgtct actaaattag taaatgcatc aattttatat tcttcatcac
     1261 taaatgcagt caagattaga attggtaaat tgcttttctt tctaatttct ttcaacactt
     1321 ctaaaccatt tataaaaggt atctgaatgt ctaagataac caaatttata tcgctattaa
     1381 attttgacaa ggcttctctt ccatctttag cttgaataac agtatatcca aattctgaaa
     1441 gatattcact tattccctct cttatcatat tatcatcttc aacagttaat attttcatat
     1501 atacctccac ttaagcctat acactagata ttatacagca tattatggct ctataatatc
     1561 tagtgttggt gacgccttct gttgccaata ctttttcttt tttttaataa atgcaaacag
     1621 acaccttgaa atcctttaga aaaaaggctt ctataatatt ttgtgttgtt ggaaaatcga
     1681 aggcgatttt ctactaacac tttttcttcg tttcaactca aaaagagact cacttttggt
     1741 aaaatttagt cgaccaaaac atcaccctac caaaggagtc gctctatgga taatcatact
     1801 agaaaattac tcggattaac agataaacat ttattttttg atgaagaatg gctgattgaa
     1861 aaagaatata aaggcgttca agcgcagttt attaaaggga gattggatga ctcaccttcc
     1921 cattgtgaac actgtggatc gattcgtatt atccgcaacg gttcctatac gacacgtact
     1981 cagatgttga aagtcaaaga aaagttgact attttagaat taaaacgtac ccgttttcta
     2041 tgctatgatt gtgggcagac cttttctgcg aaaacggact tagttgatga acatcatcaa
     2101 ctaacaaagg aactgaagca ggcgattctc atagaactgt acgaaaatca atcacgtaat
     2161 ctgaaaatca gaagaagtac cgtcggctca agcgatattg gaaactctta ctcaaagatt
     2221 ccacgacatt ggagccgctc aaacggcatt atcatcgact gtttaaacga ccaattagtc
     2281 aaacagaaat cgtcgatgaa ccgttgtgca caatggtgtc ctgaacgtac agcgacacct
     2341 tccatatcca gagcagtcgc cacatcacgg ggatggatgc catccaaatt aaaagcaacc
     2401 acgccagtat gatgttcagg atcttgcggt ccgtaaatgg tgataccgcc gatcgcttgt
     2461 aatttaggta aaacgtaggc aaccagttct tgctcataac gatgaatatt ctccatacca
     2521 atctcattca agtaatcgat tgctcctgca atgttcggag taccagcttc aaatttccat
     2581 ggcagctctt tccatgaact atcgtaaaga ttgacaaaat ctatcatttc accgccgaat
     2641 tcgactggct ccatttgttc taacaattga cgtttgccat ataatacacc gattcctgtc
     2701 ggcccgcaca ttttatgccc gctaaaggcg aaaaaatctg catccaacgc ttgtacatca
     2761 actgtcatat gcggtgctgc ttgggcaccg actggactat cctcaactac aacagcctgt
     2821 tcaaccctca gccgtttcaa gcacaacaaa taaccttctg gatctggttt gccgacctga
     2881 agatcatcga tcgtcacaaa ggaatcaatt ttttccttaa tcatcggatg tgcttcaaaa
     2941 aaatagtcta gctcccgcag ttcagaattg agactactcc gattttccag tgctgctgat
     3001 ccgctgctca aaatgttcag catccactgg tctcccaacc gcttgactta tcccttcata
     3061 aaaggacagt tctgctgccg tcccaacacc ttgttgatag tctgcaaaag gaattttata
     3121 gccgaactgc tccagtaaat aggcttcagt aaatagtgat gaatactctc gtaaaacatc
     3181 cattcactgt caatcatcgt cccatcaaaa tcaaaaataa gtgctttggt catcttcttt
     3241 cccccttacc caaaacaagc aaaagctgtg tcctgctctc tctacagtat cagcataaaa
     3301 actgccgtct gaaaagtcaa aggtgaaaga aatgtggcgc tataatttct aggactgata
     3361 gtaaatcatt ctatcagtcc ttttgagcat aaaaaaacaa aacatacaga agctccgtta
     3421 gaattgaatc accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcttatc
     3481 aaactattac taggaatcaa agatggaaac attcaaatca ctcatgtcga gaagaaaaaa
     3541 atacattcaa tcaacagtat cgttgtttct gccacgctca ttaaaacgat tcagccctgt
     3601 cctcagtgta aacaggtgaa tcaacatggg ctgatcgtta agaacggaaa gaaaaaatcg
     3661 ctggtccttc tcaataagtg tgcgaatcaa cggacttatc ttgctttggc aaagcaacgg
     3721 tacctctgtc gtggctgtca cacctatttt acggcgaata cctacattgt tgaaaaaaac
     3781 tgttttatcg ccaaacaagt acattataaa atattggagg aattgactga aaaacaagca
     3841 atgacgacga tttctaaaca ttgcggcgtt tcttggtcaa ccgtttcgcg aacactcgct
     3901 tctctgctcc ccatgacaaa cgtgaaaata aattggttgc ctcgctgttc attggtagat
     3961 gaatttcgtt cattgaaaaa tcaagtcggt ccctattctt tttcttgtat ggatggcgat
     4021 actgggaaat tactggatat tcttccttca cgtaagaaga aagatctcat ttcttatttt
     4081 atgcaatttg aacgacgtgc acgtttaaac gtaaaaatac tcgtaacaga tatgaacgcc
     4141 tcttatgctt tgctgataaa agagtgcttt cctcatgcca aactcgttgt ggatcgcttt
     4201 cacatcgtca agcacctcat tcgcagtttt aaggatattt gactatgagt catgaaaagc
     4261 ttcgaccgaa acgatccaat acaagccaaa cactatcgac aagtgaaggc tttatctcga
     4321 cttctgatca aacgacagga cacgcttgtt tatgataaat ggacaaaatg gcgtaatttt
     4381 ggttgggcct atttaaccga aagtgaagtc gtagaacgtc tactttctac ttctgatgaa
     4441 ctacgaatag cttatgctta ctatcaagaa attcttcaag ctttttatga caaagaagcg
     4501 gacatcttct ttcagcttgt aaaaacaatg cccaagtcgg ttccaagaga actgcatcat
     4561 ataaaaaaag cgtttataaa ctatgaatcc ggcattcgtt tagccttaga gttgccttac
     4621 tcaaatggca aaatagaaaa tctgcatacc cacatcaaag cattgaaacg aatagcttac
     4681 gggttaggat gagaaccaga attttcttaa tcaatcatct gatcacttat taaacataaa
     4741 aaaacatcta aagcagaaaa tcaattctac cttagatgtt cttatatcac tctatcagtc
     4801 ctatttgaca aagagccaga aatgtttgcc gactatacac tagctttgtt gtgcaaaatg
     4861 ctgagtatca atttccacag ggattgctag aatatctttc agctgaatgt ataaggcatc
     4921 gctaattttc tcttcccgat cgttcaaccg atccactaaa atttctttgc cctccagcgt
     4981 ttgaaaaaca taccgttgaa tgttgcctaa aataaatgat tccttccata gaaccggaat
     5041 cttaaccccc tgaggaaccg gttcaaattg aatcagctcc ggtcttaaat aataaatgca
     5101 gcgattgccc ttagcggaaa actcgtgggt atactgattc agctcatacc attcgaaacg
     5161 attatagttg ccgataaatt ccgcaacaaa tcggctctga ggattctgat acaattcctt
     5221 cgaggtggaa acctgagcca cccgcccagc ttccatcaca aaaatccgat cactgattcg
     5281 catcgcctct tcttggtcat gagtcacaaa aatcatcgtc atccctagcg attgctggat
     5341 agcgcgcaaa tcccgctgca gctgttttct aattcgggca tccaaagcac ttaatggctc
     5401 atccagcagc aggactttcg gttcagtgac caatgagcga gccagtgcca cccgttgtcg
     5461 ttgaccgcca gaaagaaact ggacattttt atctgcctgc tccgtcatat ccaccaatga
     5521 tagcatctca aaaacctttt cctgaatcac ctcaggactc agcttcttta ccttcaaacc
     5581 aaaggctaca ttttcaaaaa cagtcatagt tggaaacaat gcatactgtt gaaaaacaaa
     5641 gccgatattg cgatccttcg taggcttatc ggcaatattc tcattgtcca gcacaatctc
     5701 accgctcttt ggttgttcca atcccgtgat acagcgcagc aacgtagact ttccgcaccc
     5761 gctgggacct agtaaagtaa ccagctcacc cttttcaatc tgaatttcat caatagctaa
     5821 aatctgtttt tcatcaaaaa acagattgag atcatgtatt tctaaaaaca ttttccgtca
     5881 caactccttt aagggcttct ttttcgactg cttctcttcc ctcgtctgct taagggacag
     5941 ctgttgcttt tgtcgataag tcagcataaa ggtagttaaa gcaattccca ccataaacag
     6001 caaatacaaa accatcacag cgctagccaa atggccgttt tcgttcatcc gccgcagcat
     6061 atagatgcgt accgtctgaa atcgaccgcc aatcaacagg ttcgtcagca tgtactcccc
     6121 aaaaaccgaa gaaaaaatca tcagagaaac tagggtcaca ccgatacgga tgtttggaaa
     6181 cagcacctgg ataataatcg tagacatcgg ggcgcccaag gtttctgctg catccactaa
     6241 ttccttcaga ttaattaagc gcatctggtt gttaagacta agatagacaa tcggcaacgc
     6301 cgtaataaac aatgcaccta ccaacactac aaacattggg ataccagttt cagcatacgt
     6361 tcgaagcaaa gccgttacca aaatcactcc tggtaaagca tacggcagca ggagcagctt
     6421 ttccagccaa cgattcaaac gagggaaata caaatgaatc caaataatgg tgggaatcat
     6481 caacaacaga atcgtcacca agaccacacc tgctaataaa agtgagcgac ccaccgcagc
     6541 aataaattcc ctgtccgtca gcagctgttg gaaccattgc aaggtaaaat cactaggtaa
     6601 aagcgatttt gaccatttag tggagctggc atacaaaatc gttccaataa tcggcagcaa
     6661 gtaatacagg acaaagcaga acaaaatcgg ttttattaac tttttcacgt ctgcaccacc
     6721 ttgcctttct tttgaataat caacattgcc gttaccagca ggacacctaa agcgactgcc
     6781 aacgcactgc ctaaattagg cttggcgaac acatctcccg acactaaagc cgcaattcga
     6841 atcgttaaaa gatttacatt actgctggtc agggcatacg ctgtttcata ggtccccatg
     6901 ccattagcaa ataagatgac aaaggtgctg gcaatcgatg gtcgcaaaaa aggcaaagcc
     6961 accttgaacc aagcatacgc tgatgaagcg cccagcaatt gtgcggactc cagccattcc
     7021 tttttcactt ccttcatgct gggatacaaa aacaagaccc ctaacggaat ttggaaatag
     7081 gtatacgtga tggtcaatcc ccaccaagaa tacaaattga agtccgctag aaatggcaaa
     7141 atcattttca gcacacctac attccccaac aaaataataa aagagaatgc cagcggaatc
     7201 ccagcaaaat tagctgccaa attggaaaca aaggtgattt tctcctgcaa tttagtcgat
     7261 agattcatta agcataaaca taaaataaac gtcccgatta acccaatcac actggagctg
     7321 acagcaatca gtacgctgtt aacaaaggcc tgtgtgtaat atttgttgct gaaaatatca
     7381 ctgtaattgg ccaatgtcca ctggttagtt aaatcactgt gcaagctgca cagaaccata
     7441 aaaagcagcg gcagcagtaa aaatgcacag aagataaagg aggcaggaac cagaaccaag
     7501 cgttttttca tctctttcat cccctcatca attcttgaat gtcctgcatt ttctctgacg
     7561 gtgcaattcc taataaccag caaaacaatg gagccgccag cagctgtggt acagtttgtt
     7621 ccttcagctt ttggcgttta tttgaaatca aataaaaagg aacttcacga tgtgcagcca
     7681 agctgccgcc atgcagtccg tgcgcatcca tcccgtgatc cgcagtcacc acgacttgat
     7741 aacctttttg cagccattca ggaaggtaaa tagccaataa tctgtccgcc tgattaaccg
     7801 ctgctgcata ttcatgagaa tcggcggtaa atttgtgacc gacatcatca atattcatcg
     7861 aatgtacgac caaaaagtct ggctgatact gattcactaa ataatgggca tccgcaaata
     7921 aatgcgaatc agggtaagtg tcctcactat aaaagatgcc atgctgaatc agtcgctcct
     7981 cctgatgctg gatgcgatca accaaacgat tatagggggc ttgattgtac aactcgctgt
     8041 accagtggta agcggcggct cctgtttttc cgccagcttc cttaactaat tcaaacagcg
     8101 accactcttt ggatcgctga gcatggtaat tactatagat gccattttca taggctggaa
     8161 cgccggtcat cagtacttcg tataacggac gtgaattgct gggcagctca ctttttacct
     8221 tcaataagct tgcttgctgg tgctcaacta aatgattcat gaacccaagc tgggcgacgg
     8281 ctgtgtcgta gcgacagccg tctagtataa ctacaatcag tttgtcaagt ctactttgca
     8341 taggtcagta cctccgcctg ccaaagctct ggaagctcta cagaggtctt gttccaagct
     8401 tcattgtctt caactggcac cgccgcttca taagcatcct ccggcagtaa tttttcagct
     8461 acttctttag gcagctctac cttgcgaata ggtcgcgcat agccctttgc caaattgatt
     8521 tgcccttcat cagataaaat atattcccgt gctagctttg ccgcattggg atgaggggca
     8581 tttttattga ttaaggttgt atacccgcta atgatggagc cgtctgttgg cacagttacc
     8641 tcaaaacgct cggagtcaat ttggtcacga taatttagtc cattgaagtc ccaaacaact
     8701 gccacatcga tttcaccttt ttcaagattt gctacgctgg catcaacggt ggacaaacgg
     8761 cctttttctg ccattttttt gtagtaattc agacctggct gaatatcctt ttcgtcacct
     8821 ccgttggcaa tcgccgcagc caatacagca aattgtgctt ggttggcaat cgtcacatcc
     8881 cccacgctaa cttgatagtc accctcttgc agctccttcc agctagtcgg tgcttgcttc
     8941 acattctctt tatcggtgat aaaggaaatg gttcccgtat agcttaacaa ccaatcccca
     9001 tcatcatcct ttgcccaatc cggaatttcc tcccaatagt tggtcttata aggtaaggtt
     9061 aactctttgc tttcagccaa cggaccgaag ctgataccga catcaccaat atcggcggtt
     9121 ccattttttc cttctgattc aaacttagcc aattcttctg cactggacat atccgtatcc
     9181 acgtgactta agccgtattc tttttcaagg tcgttccaag tgcctaccca atttgcccaa
     9241 gtatccggca ttcccaagct ggcaatctct ccttcttcct tggctttttc tataatcgta
     9301 tctaaagagt cgtctgccgc agccgattgc ttcgttgctt cacctgagcc gcagcctgtc
     9361 agcaggatac ttactgttgc tgcacaaaat aaaccgatag tcatgttacg aattttttca
     9421 ttcattttca ttcattcttc tcccctttcg ataattccat tctaacaaag ctttgtaatg
     9481 agagaatcag tttcttgaaa aggtttcatg aacaatgtaa aggttgtata tggtttgtaa
     9541 atttcgacga attcccgtta accttctaaa gcttttgcgg tatgatagta gaaaaaagga
     9601 ggctccctat gcagtattat gatcagcatc tccacacgta tttttccttt gattcagagg
     9661 aaaaatttga aaactattta gcctatcagc cggagttttt cgtttctacg gatcactttg
     9721 atttaaacaa tccatctacc ggaaaagatg acattccgga ttatccagcc tatataaaca
     9781 aactggactc tttaaaagaa acctattcca ccaaatttct gcgaggtatc gagattggcg
     9841 ttgttccagg acaagaagag cggatcgtgg attacctcgc tcagcatcct tatgatttaa
     9901 agctggtaag tattcatcaa aatggccgct ttgattatat ggacgatgtg gttttaacca
     9961 aagatcccat tcaaactacc aaggaatatt ttgatcagat gttgcaggtc ttgacggatt
    10021 ttccagaagg agatattttg actcactttg attatggatt acgccgcttc accttcacag
    10081 cggaagaatt cgcggagcat tttgaggctc agctgctaac gattttcaaa aaggttgttg
    10141 aacgagaatt ggcgatggag ttaaatgcca agagcttctt gaaatacggc aacgaagacc
    10201 tttaccgtta tgccgttcct ttgtatcaaa gtgtcggcgg aaagctcttt accttaggct
    10261 cggatgctca tgttgccgaa gattatcagc tgggctttaa aaaaatgagt cagctgttgg
    10321 cagactgtca ggtagaaaag ttgctagtca ttcaaggaaa agaacgccgc ctgacgccgc
    10381 tgccgatttt gtgattccta tataatagaa gaaagtggtg aaaaaaatga gcaacctgtg
    10441 ggactacaat caagaggcac ccatccatta tttaattgcg cgacattggg atgcgttgaa
    10501 aattgaggcg gtttgccgat ccttgttagc agccgttcca aaacaacaac tggaaaattt
    10561 tttggtggct gacagcttgc agcgggaaaa agttcaggct tattttgcag cctttaaaga
    10621 tcagcctttg gaatatttgc atgcgcagtt tcatcttttt tatcaagtag ccgccccaga
    10681 tgactacaac gatttacgag gacagctgca attgaccttt caagcggatg aaaccgccta
    10741 taccgtttta ttgggaatgg ctcgccttgg tgatcaggcg aaggtcgagt ggcggatttt
    10801 cgatatttga tggcaggatc aaggcgtttc agttgcaaac cttcctctct ttcaattaga
    10861 aaaaccgcag caggaagaag tttgtgtgct ggtcaccgat gctggtgaaa ttcgtctccg
    10921 cttatttcct gaccaagctc ccctggctgt agccaactgg cgctccctag ctgagcaagg
    10981 cgtttatgac aacacacctt tcggctcgtg tcattaagga atttgttatt caaggtggcg
    11041 ctttggatgg ttctggtgat gaagctgttt ccagctatga gggctttttc gctgatgaag
    11101 tgcatcgcgg actttatcat tttaatggcg ccttggcctt aggggaccac ggcccccaca
    11161 ccaacggcaa ccagtttttt attgtgcaaa acacaaaggc gcaggctgac ctgctaatgt
    11221 aaagcaagca tacttggagc ttggaggact accggagctg gatggtcgtt atactgtttt
    11281 tggacaggta taccaagggt tagaaatcgt agcagcgatc gcagctcagc cgacaaacag
    11341 tgaagatgta ccgattgagc cagtgttcat tcgacaaatt aacttcgaaa aaacatcgta
    11401 aaaagacact cactgacttc agcaaaagct ctattcttga gctggctgca gctagtgagt
    11461 gtctttcatt tatccataag ataaagcttg gattcaataa ataaataaat gttttggtta
    11521 aagctgtgac cagcaaatga ggaaccgtgc gagccagttg atctgccaat gcaaacattt
    11581 ctttaggtga aggcgctggc gtgtctgcca acggatactc cagtcccaat gcttggtaac
    11641 cgtgcatacc atagcagtgg tacggtagta attctaacgg tacgaaccga tcaagatctg
    11701 ccacaaaaca agcaatcgtc agcagctcgg tcaggttcat attgatacct ggaaccacag
    11761 gaactcgaat tcgcacttcg cagttaggat actcaattaa aaagcgacga atattcttca
    11821 aaatttgttg gttatctaag ccggtccatt gtttgtggag cttgctggta aacattttta
    11881 catccacaaa caatgtatcc acatagggag ctaccttttg gatttcatta taagcaccac
    11941 aaaagctggt ttccagtacg gtattgattc caatgtattt cgatttttgc aaaatttctt
    12001 tagcaaaatc cgcctgaacc agtgcctcgc ctccgctaat ggtcactccg ccatccgagt
    12061 gaaagtaaaa aacagcatcc ttctcaatct catccattac ctcttcagcg gtcatggtct
    12121 ggccaaatcc tttttcaatc tgcttgcttt gtgattcggg aatcgaacac caggcacagc
    12181 gtaatgggca gccctttaaa taaacaacgg tccgtaaacc agcaccatca tgtagagaac
    12241 cctgttctat tcttaatact gttccttcca tatagatttt tgaattacac actagtttaa
    12301 actaaaacac acaaggaaaa acttaccatt taatcataaa attagctcat ataaatatac
    12361 taggatgtaa aatagactgg tcaaaatctg gtcaaaaaag aggactaata caggtagtcc
    12421 tcttttttgt tattttaata ttctaaatca ttttttattg gtttattccc ttattcgatg
    12481 tagactcaaa tcactatatc actagatatc aaacggcgtc atacggaatc aaaaacatca
    12541 aatttgacct gttaaatggt cattgaataa cattcaaaat caataaaacg gagtcagaca
    12601 cggagtcaaa aacgctgaga gtaaaaaata tatacagcct tgtaggtaaa gtaccacagg
    12661 gctgtatatt ttttatttca gattacctta aatgattcca tttacttttt atctcatttt
    12721 ttcctactag ttagtatcaa atactctcct attttctcaa taaactattt ttggatacat
    12781 ggtatctgaa acagctaaaa ccttgtcatt acagcaaaaa atcacgttat ccagactgta
    12841 gacaaagtcc attttatgga tgatgtctac agtcttttct tttataatag atttatgaaa
    12901 acgaatgcaa ggagtgatag ctatgatgtc taaaaacaat acgaatggta gaaatcaatt
    12961 tgcgatgctt acgattgatg atctagttcc tcaagatcat ctcgttcgaa agattgatgc
    13021 cgcgcttgat tttgaattta tttatccaat cgtcgaagcc acttactccg a
//
LOCUS       DAYYGY010000080        13053 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000080 DAYYGY010000000
VERSION     DAYYGY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.081"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1072
                     /locus_tag="ACHA52_002319"
     CDS             149..1072
                     /locus_tag="ACHA52_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8940987.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1078..2331
                     /locus_tag="ACHA52_002320"
     CDS             1078..2331
                     /locus_tag="ACHA52_002320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8940988.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="ACHA52_002321"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="ACHA52_002321"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8940989.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4625..5578)
                     /gene="menA"
                     /locus_tag="ACHA52_002322"
     CDS             complement(4625..5578)
                     /gene="menA"
                     /locus_tag="ACHA52_002322"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8940990.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5591..6694)
                     /locus_tag="ACHA52_002323"
     CDS             complement(5591..6694)
                     /locus_tag="ACHA52_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8940991.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6784..7719)
                     /gene="pplA"
                     /locus_tag="ACHA52_002324"
     CDS             complement(6784..7719)
                     /gene="pplA"
                     /locus_tag="ACHA52_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8940992.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8021..9955
                     /locus_tag="ACHA52_002325"
     CDS             8021..9955
                     /locus_tag="ACHA52_002325"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8940993.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10412..10828
                     /locus_tag="ACHA52_002326"
     CDS             10412..10828
                     /locus_tag="ACHA52_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8940994.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10842..11408
                     /locus_tag="ACHA52_002327"
     CDS             10842..11408
                     /locus_tag="ACHA52_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8940995.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11431..12417
                     /locus_tag="ACHA52_002328"
     CDS             11431..12417
                     /locus_tag="ACHA52_002328"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8940996.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12572..>13053)
                     /gene="scm"
                     /locus_tag="ACHA52_002329"
     CDS             complement(12572..>13053)
                     /gene="scm"
                     /locus_tag="ACHA52_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8940997.1"
                     /translation="DSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         4066 a         2370 c         2695 g         3922 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtattgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     4321 tttccatttg tttctctaat actcaggaca aaacactgat tttacaacct ctgataaacg
     4381 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat
     4441 tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc tcctgataaa
     4501 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc
     4561 tatttttatt tggctgttct tattacttga tgcagggcag ttttttcaca acctcttcat
     4621 gctgttaaat ggcttgcata atgattgtta gtaatagccc caatacataa ctggcattga
     4681 ataagaccat atttttgatg gaatagctga aactgatagg ctggggtaac gtttgcttga
     4741 attgatccaa atgcttcttc acaattggca aggtcaagaa ggtgaccaag atcggccatt
     4801 catagatacc gctcaacaac cccattaaaa tcacgaaata gcaagcatac atcaatagct
     4861 ggaataagac tgttcccgca ggacgtccga tataaaaaac tagtgtatag cgatgatttt
     4921 caatatcacg gtcaagatcc cgcaaattat tagccaacat gatattggca atcgtaaaca
     4981 ccaagggtaa ggaagctaat acgattgctg ctatagccca aagatttcct accaaagcaa
     5041 attgtccaga tgaaaagttg actgtcagat catagacatc tgaaccgaat gtattgacat
     5101 aaacgaccat ggcaaaaatt ccaaggccca tagtcacccc actgaaaatt tcccctaaag
     5161 gcattcgaga taaaggaatc ggtccgaatg tatagaatac tccaataaaa caacagataa
     5221 atcccatcaa aagtaaaagc caacccgtat tgatcgttag aataacgccg ataacaaaaa
     5281 caaatgcaat catagctaaa atcatgtttc gaatcaatac aggagataga tttctgcggc
     5341 caatgacatt ttcttcatac ttatatactt cagaatgagc cttttcataa tccatgtaat
     5401 tattgatagc ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat aaaaaagtgt
     5461 ttagccaatg tatctcatga aaaaaagcga tagagaataa tacccctacc gcaaaaggaa
     5521 atacactagc cacctttgtt cgtatttcaa ctacttctaa aaagattttt agtgtcatgg
     5581 ttcgtactcc ttattttagt tcagaacggt cacccatcgt gtaatgggag tcctccgata
     5641 atttgaaggt gtctttgatt ggatcggtca catatacttt atcgtctttt gtcacgaaga
     5701 tcgcttccgt cccgttattc aattcgcttt cgacgtattc cagtcctttt ttcacaccca
     5761 ttgaaaagac tgctgtagac aacccgtcac catcaattga tttatctgtt atgatcgtga
     5821 cacttgcgat atcattgtcg aacggataac ccgtttttgg atcaaacaaa tgatggtatt
     5881 ttttgccatc tacttctaaa taacgctcgt aaattccaga ggtaactaat gttttgttcc
     5941 gttctttaat agaacctaaa acagatccgc gagccatgtt cggatcttgg atgccgactg
     6001 tccagtcttg gttttcgcct cttgggctgt ggcctaagac atagacatta ccgcccaagt
     6061 caacgatggc agtggtcact ccttgttttt tcaagacttt cactacttcg tccgtaatgt
     6121 aacctttggc gattgcacct aaatcgatga tcattccttt ttcttttaga taaacagttt
     6181 tttctttgtc atttagttcg atcttatgat aatccactaa tttcaatgcc tgatcgattt
     6241 cttcttggga aggttttctg gcgtcatcaa aaccgattcg ccataattgt gtgattgcac
     6301 caatcgccat atcgaatccg ccttgagaat cttggctata ttcgtaggcc cgcttgacta
     6361 acgtgtagac gtcatcagaa acttttactg gtttgatccc agcttgctca ttcacttcat
     6421 cgatctcaga acctttttga ttgatcgtga ttttatcacc aagttctttt actcgatcga
     6481 aggcaggttt caatgctgat tctttgcctt catcatacac acggatacga acgtaagtcc
     6541 ctaacaagaa ctgttcatca gaatatggat ctttcaatat cttggtagct gctgaacttt
     6601 cagtggttgt tttatttgct gagttgctgc agccagttag caccataaaa gagatgccca
     6661 gtgtgattaa ggaaaatagt agttttttcc gcatcttttt cctctcttca ttttgaaagg
     6721 tgagacgact gcctaatctg gctatcgtcc caccctccaa cttttattag tatgattatg
     6781 tgattatttt tctgttacga tattatctac ttcgattgtt tgagtgtcgc ctttttcagc
     6841 tgcattcact aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg tagcacctgt
     6901 aacaacttct acattagcag gtgagccatc ttcttcgccc attgctttga cgaattcgtc
     6961 gttcagagta gggatgtaag tttttggttc tgtaccgctt tttgctttca tattttcatt
     7021 atattctgta tcatcttgtt ttgatttacc gtctttgtcg atataatcaa agtttgattc
     7081 agttactttg ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt tagagtagtt
     7141 cttttcttca agcttgtaag tgccgtcttt caaatcagca ccattgtcga tttcgattgt
     7201 atctgtgtta ccagcttgag cagcttggat caattgttgt gcgtagtttt ggaagctttc
     7261 agatgagtga gtagctccag ttacgacttc cacaccgctt gcgctttgtg cttttaagaa
     7321 ttggtcattc aactcaggaa tgtatttttc tgggtttgtg ccagattttg cttccatgtt
     7381 tttgttgtat tctgcatctt ttgtttttga ttcgccgttt tcattgacgt tatcatattt
     7441 tgattcagtg attttgccgt cttttacaac catttcaaag actgcacggt aaccatttga
     7501 ataatttttt tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag ctacaacttt
     7561 tgcagatgat tctgttgtgc tttttgcagt cgaagattgt gctgtagatg attcagaaga
     7621 agagtttgag cttccgttgt catctgcgcc gcaagcgcct aacactagag ctgaaaatgc
     7681 taatacggca aatccagtta caaaacgttt tttcttcatt ttaatacccc accattccat
     7741 ttctagtcca caatttttct ttgtgattca ttatacattc taatcttttt agcctataag
     7801 tcaactattt ttgataaata acccatgtaa tatttcaaac gttttctttc agcaaatttc
     7861 gtgaaaaaac gctttatttt aaaatttgaa aaaaatccaa aaggagttag acaaaagaac
     7921 agaacatcta tataatatat ggagattgtg taataagtat caaactaaaa gaattatcat
     7981 atattcatta tatagatagt ctataaggag cggatgatca atggcaaagc aaaatattgt
     8041 cgttgtagga gcaggatatg ccggtgtttc agctacaaaa tacttggcaa agaaactgaa
     8101 aaaagaagat gtagcaatta ctctgatcga tcgtcattcg taccatacta tgatgaccga
     8161 gttgcatgaa gtagctggcg gacgtgtaga accagaagca atccaatacg atctgcagca
     8221 tttgtttgct cgtcaaaaga atgtacaact tgtcacagac actgtaatcg gtatcgataa
     8281 agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct tttgatcagc tgatccttgg
     8341 aatgggcgga gagccgaatg actttggtac acctggtgtc aaagcacacg gattcaccct
     8401 ttggtcattt gaagattctg taaggattcg ggaacatatt gagaagacag tagcaaaagc
     8461 tgcattggaa ccagatgatg aactgcgtaa agccatgctg actttcgttg tctgtggttc
     8521 tggatttacc ggtattgaaa tgattgggga actgatcgac tggaaaaatc gcctagccaa
     8581 agattataaa ttagatccaa acgaaatcac actgatggtc gtagaggcaa tgccgacgat
     8641 cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc gtaagcacaa
     8701 cgtggaatta ttattgaatg caccaatcgt agaagtggct gctgatcata tcaaattgaa
     8761 agacggacgc aacattccga ctcacacact gatttggaca gctggtgtca aaggaacttc
     8821 tgacgctgct gactttggat tagaatcagc acgcggacag cggttgatcg ccaatgaata
     8881 tatgcaggca aaaggctacg aagataaaaa catctatatc attggtgatt tagtctttta
     8941 tgaagaattc ccaggaacac cgacaccgca gatcgttcaa gctgctgaac aaacaggaca
     9001 cacagccgca gcgaatatcg tggcttcgat caaaggcgga gaaaagcata aatttaaagg
     9061 aaactatcaa ggatttatgg tttctattgg tgccaaatgg ggggtagcta acctatttga
     9121 taagatccat ttaagcggat ttttagctat cgtaatgaaa catatcgtga atttgaaata
     9181 cttctttgat attcgttctg gttattatat gttccaatat ttaatgcatg agtttttcca
     9241 tatcaaagat gatcgcaacg tgactcgagg acattcttct cgttacggca atgtcttatg
     9301 gagtgtgcca cttcgtattt tctatggttt agtttggttc gttgaagctt ccaaaaaaat
     9361 cattgggaat ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat catggttcac
     9421 tggaaacagt gtgttccctt ggacatggga atacaatgta acagatgttg ccagtggtgc
     9481 ttctgctgct ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg ccggagaagc
     9541 agcgactcaa gctgcacatt ttggtttgag ttatgcatac ggtgaagaac caatggctgt
     9601 gattgagaag tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc caaacaatga
     9661 tgtagcaatg ttcttccaaa aattcatggt attctttgaa attgccttgg cattagcatt
     9721 gattgctgga ttattcacat ggctatgcag tgcgacaacg attgtattgg tgatcacgtt
     9781 ctgcttatca ggcatgtttt actgggtaaa catttggttc atttttgttg ctttcgcatt
     9841 gatgaacggt tccggacgtg cgattgggtt ggatcgctgg gtaattccat ggctacaacg
     9901 tacactaggt aattggtggt acggtagacc aagttcccag tacggcaaga aataaataga
     9961 taaagaacgt tatttgaatg caagatttca atagaaaggg gctgagacaa accgtcacag
    10021 cctccttttt atgtaaaatt aaaggaataa gatgcccgag ataagcctcc atgagcaaga
    10081 cacaggtgtc acaacctctg ttacaacctc ttgttataaa cggtgttcca gaagtagctt
    10141 cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag taattttcgt aaattccttc
    10201 ttatttcttg aagttgccca cttctgtcac aacctcttgt tataaacggt gttccacctg
    10261 ctgcttctcg gtaataagcc caaagaatta gaaaacatgg aaagctgttt tctattcttc
    10321 gttcttatta ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat gctaaaattg
    10381 ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg ctgaaaaaag
    10441 tctgctcaaa ccttgggatg tttttattat tttgcttttg actgttcttt cttttttacc
    10501 agtcatgatt tttggtcttc agcaaaacac cgcaacagca gaaaaagaag cggtactgcg
    10561 tgtggatggc acagaaatca aaacattccc tttgatggct ggagaagaga gcaacacata
    10621 tacttacaaa gatgaacacg gagattataa cttgatcgag atagatggcg atcgagtacg
    10681 gataaaagaa gcagattgcg atgaccagat ctgcgtacgt cgaggctggg caacgaaaaa
    10741 tggcgaaacc atcgtctgtt tgccacataa attagtgatc gaagtccgtt caacagatgg
    10801 aggtgacgaa gacagtctga tttattagac cttcacacac catgagtaaa ttacaaaaaa
    10861 tgatttatat ttcgctactt gttgcacaag gagtgatcat tggtttattg gaaaatatga
    10921 tcccttatcc tttttctttt gcgcctggtg caaaactagg cttagccaat ctaatcacga
    10981 tcgttgccat ttttaccatg aaaaaacgag acagcttctt actactttgg ctacgactat
    11041 tcctgactac tttgctaggc ggaacgattt ccacattcct ttatagtatg agtggtgctt
    11101 tgcttagtta ttttggaatg ttattggtca agcaattagg accaaaacgt gtcagtatca
    11161 tcggaattag tgcggccggc ggtttcatgc ataatgtcgg acagcttttg atggcttctt
    11221 tcattgcaca atcatggaca gtcatgttgt acttgccagt cctttcagtt ttagggattt
    11281 tatcagggat tgcaatcggg atcgcagcga attatctttt acgacatgtg cagacacttc
    11341 aacgattcca aaaagattat gagcagggaa ccaaacaaaa atggagccca atgtttacca
    11401 aaaaataatc gtcacacaag gagttttttt atgaagctac atccaatgtg ggaaaactat
    11461 cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac tgtccgcctg
    11521 aaaaacaaac ctgtcgaaca agcaatcaaa gaaacgatcc atgccggcgg aaagctgttg
    11581 cgtcctgcct atcagctatt gttctcacaa tttggaccag agcaagatcg agacaaagcc
    11641 atcgctttgg cagcttctat cgaaatgctc catacggcca cactgatcca tgatgatatc
    11701 gttgatgaag cggatctgcg acgtggtcag cctaatatcc gtagtcagtt tggtaatgtc
    11761 gtttctgtct atgcaggaga ctatttattt gtctgctgtt ttaaactatt gtctgattac
    11821 tccacttcct tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt attgaatggc
    11881 gaattagggc agatggatga tcgctataat tatgaacttt ctgtaaaaga atatttggaa
    11941 aatatttcag gtaagacagc tgaactgttc caattaagtt gttctgttgg cgcttttgaa
    12001 agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc tatcgggatg
    12061 gcctttcaga ttatcgatga tattttagat tatacaaaag agagccagga aatcggcaaa
    12121 cctgtactag aagacatgcg tcaaggggtc tattctttac cgttgatcta ctctttgcaa
    12181 aaaaacaaac caaagctact accttattta gagaagaaag cggcaatgac agaagaagat
    12241 gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg tggaagaagc taggaagtta
    12301 gcggcctctt acacagaaaa ggctttgaag gaaataaaga aattacctgc cacctctctt
    12361 cgaacaaaag agaatctctt ttcccttacc caactgattt tggatcgcaa agactgaata
    12421 gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt ttttgacaaa
    12481 ttgaattgta gttcttgtat ttatgataaa aagcaaaaat aaagagcaac gtccgttagc
    12541 gacgttgctc ttttttatct tgaatatttt tttacctttt cgatttctta taactcataa
    12601 aagtgattct taaaccgatt acaagtaata taacgcctgc gagtgttatc caattattcg
    12661 attgacttcc agttttagga agtgctcttt ttgatttgtt tccattgtca ttgcttgttt
    12721 tattagttaa gttaaagttc gtcctgttat ccgaacttgt ttttgagaag gagacacttg
    12781 catgacgtgt atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa gttgatgaat
    12841 ctgagctatc acttgtggta gaggatgtat ctgtactgtc acttgaagct gacgtgtccg
    12901 tgctatcact tgtagtggta gatgaatctg agctatcact tgtggtagag gtagtatctg
    12961 tactgtcact tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat gtatccgtac
    13021 tgtcgcttga agctgacgtg tccgtgctat cac
//
LOCUS       DAYYGY010000081        12897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000081 DAYYGY010000000
VERSION     DAYYGY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.082"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..1426
                     /gene="efmA"
                     /locus_tag="ACHA52_002330"
     CDS             140..1426
                     /gene="efmA"
                     /locus_tag="ACHA52_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8940998.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1564..2163)
                     /locus_tag="ACHA52_002331"
     CDS             complement(1564..2163)
                     /locus_tag="ACHA52_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8940999.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2567..3688
                     /gene="mnmA"
                     /locus_tag="ACHA52_002332"
     CDS             2567..3688
                     /gene="mnmA"
                     /locus_tag="ACHA52_002332"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8941000.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3934..5355
                     /gene="arcD"
                     /locus_tag="ACHA52_002333"
     CDS             3934..5355
                     /gene="arcD"
                     /locus_tag="ACHA52_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8941001.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5369..6487
                     /locus_tag="ACHA52_002334"
     CDS             5369..6487
                     /locus_tag="ACHA52_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8941002.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6547..7839)
                     /locus_tag="ACHA52_002335"
     CDS             complement(6547..7839)
                     /locus_tag="ACHA52_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8941003.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7989..8549
                     /locus_tag="ACHA52_002336"
     CDS             7989..8549
                     /locus_tag="ACHA52_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8941004.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8678..9055
                     /locus_tag="ACHA52_002337"
     CDS             8678..9055
                     /locus_tag="ACHA52_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8941005.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8988..11060
                     /locus_tag="ACHA52_002338"
     CDS             8988..11060
                     /locus_tag="ACHA52_002338"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8941006.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11271..12218)
                     /locus_tag="ACHA52_002339"
     CDS             complement(11271..12218)
                     /locus_tag="ACHA52_002339"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8941007.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12464..>12897
                     /locus_tag="ACHA52_002340"
     CDS             12464..>12897
                     /locus_tag="ACHA52_002340"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8941008.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4158 a         2339 c         2749 g         3651 t
ORIGIN      
        1 tgatttattg tttctaagta aagaaattta aattgaaacg attgttattt agggaaaaag
       61 gtattttttg aaaaaagaat cccgtataat gattgttgtg caaacgctat acaaaatgaa
      121 aaagaaaaga gggatgttta tggaaaatga acagtcggtc gttttaacga attggaagag
      181 aaattatttg ttctttttat cgggacagtt tttgtcagga atcactagta tggtcgtcca
      241 atatgcgatc atctggtatt tgacaagaga aaccggttcg gcgacaattt tgagttttgc
      301 tactttacta ggaatgattc ccatggtttt actaagccca tttgtaggac cgctagtgga
      361 tcgctgggat aaaaaagctc ttttgattgt cacggatatt attgtagcga ttttcgcgct
      421 tattttagca gtagtcggaa cgatttcaga atccttcccg atttggctag tttttgtgtc
      481 tttgtttatg cgttcggtcg cacaaacatt ccaaatgcca acgatccagt cgattatgcc
      541 gacaatcgtt ccatcttctc acataacaag gacgaatgga cagttaggaa tggtccaatc
      601 agcgaatttc atcattgcgc cagctctagg tgcggctttg ttttcagtcg ttcctgtcaa
      661 ttaccttatt ttattagatg tattgggggc agtttttggt gtcggcttat tgatttttgt
      721 gaagatccca aaagtttctc ctgaaatatt ggaagttccg cttactattt ttaaagatgc
      781 aaaatttggg ctgcagcaat tgatggataa taaagggtta tggtacataa cgatcaatgg
      841 tgcatttgtg atgctgttgt tcatgccagc aatcagtctg tatccattga tgacgctaga
      901 ttactttggt ggttccgttg gacaggcggg agcagtggaa gtagtctacg cagtcggaat
      961 gctcttaggc ggtgcactga tcagttttat aggaacatgg aaagaccgaa tgaaacctat
     1021 tattatagcc tacatcatta tgggcctgac gatcggtgca agcggattgg ttccaaacga
     1081 tagtcaaggg ttcttgtact ttcttatctt gaatgcagga gcaggttgtg caacgccata
     1141 tttcaataca ctgctgatgg caatgatcca gcagagttat gaatcgaatg ttttaggtcg
     1201 tgtcttaggg aacttcaact ctttgatgaa cctggcaggt ccaattggat tattatttgc
     1261 aggaccatta gctgatcgtt taggggtaga aaaaatgttc ttgttttccg gaatcgggat
     1321 tttattatgc ggaatcgttt tatttttaac gtcagctgcc cgaaattatg ataaagaatt
     1381 acagaaaaaa ctggtaaaag aacaccatga gcaaaaagac gaatagtatg gaatggagtt
     1441 gtgacagaag tcttgttaca actccatttc tgatcctaat gttcctgtgg caataagcct
     1501 actcaaatga aaaatatgag taattatctt tatttgttta atacttgata tagggcagtt
     1561 ttttcacgac ctatcgtact tcctctttgc ttcatagatt tctttgattt gttgctcaga
     1621 taacggagtg ccaaatggta gtttttcctt taagagttct ctttccggtg cgtctacgcc
     1681 tacattgaca tcgttctcga taggtacgct gtaatgatgg atatgtccat gaagattgcg
     1741 gatagtgtcg ttaggcccga gcaacatcgg ataatgagtc aagtaatatt gttgatggtc
     1801 gaatttaacg atggcaccta catcataaaa ataaaatttg ggacttccgt cagtaagcct
     1861 aggatcattt ttttctaaat aacggaaaaa agcgcgactg tcatggttcc ctttgatcaa
     1921 gtggattgtt ccttgcagtt gagtgaccag ttccaaaatt tctgcatttg ctttttcata
     1981 ttgcggatgc atagcaaggt cgcctagatg ataaacatgg tcggtttctt ttattactgc
     2041 attccagcta gtgatcaact gttggttcat ttcatccaca tttgcaaaga gccgcggagc
     2101 aaagtcgttt tttcctaata agcgttcatg gaaaaagtgc atatcactga taaagtaatt
     2161 catgtatctt ttgcctccct tttttcttag agtaaacaaa atcacttatc tgagcaactc
     2221 ataacatcta aaaagaaaag gttgtaaatg agacgtgcag ctttaagcga taagaagcaa
     2281 ttttgaaaaa tagtttttca caatttataa aattattcct tattgccgaa aggctggccc
     2341 tttttatgag tatgccaatt ttttatatcg aatgctcaaa agatgagtga ttttttggtt
     2401 gactatcatg tttgtgtact gaataggtcc agtagaacag tatttttacg acttgataat
     2461 taagggatga aattttcaga aatatgacgt ataatgtaaa atactgaaaa gttgcgacct
     2521 tcaactcgta gatgtttcag aatctaattg aaatgatggt gatatcatga cagacaacag
     2581 caaaacacgc gttgtcgtag ggatgagcgg cggtgtcgac tcatctgtaa cagcactttt
     2641 gttaaaggaa caaggatacg acgtgatcgg aatattcatg aaaaattggg atgacacgga
     2701 cgaaaacggt gtatgcacgg ccacggaaga ttataaggat gtagcgaaag tagctgctca
     2761 aatcgggatt ccttattact ctgtgaattt tgaaaaagaa tattgggatc gtgtatttga
     2821 atatttccta gccgaatacc gcgcaggacg tacacctaac ccagacgtga tgtgcaacaa
     2881 agaaatcaaa ttcaaagcat tcttggatta tgcaatggat cttggcgccg attatgtagc
     2941 tactggacat tatgcgcaag tcactcgcga tgaaaacggc gtggtccaca tgcttcgtgg
     3001 agtagacaat aataaagatc aaacttactt ccttagccaa ttatcacaag aacagctttc
     3061 aaaaacgatg ttccctctag gcggcatgga aaaatcagaa gtacgggcaa ttgctgaacg
     3121 cgcaggcctt gcgactgcta agaaaaaaga ttcaacggga atctgcttta ttggtgaaaa
     3181 gaacttcaaa caatttttga gcaactatct gccagcaaaa aaaggaaata tggtcactct
     3241 tgatggtgaa gtcaaaggac aacatgctgg cttgatgtat tatacgattg gtcaacgtca
     3301 aggactaggg atcggtggcg gaggagattc gcaagaaccc tggtttgttg taggaaaaga
     3361 tctagcgaca aacacattat atgtggggca agggttccat catcctgcat tgtatgccac
     3421 aagcttggat gcaagtgaaa tccactttac gacgaatgaa ccaatgccaa aagaattcaa
     3481 gtgtacagcg aaattccgtt atcgtcagca agacgttcct gtgacagttc gcttattgga
     3541 tgataatcgt gcagaagtcg tattcgacga acctgtacgt gcaatcactc ctggacaagc
     3601 agttgtattt tatgatggaa tggaatgtct gggtggtggt ttgatcgacc acgcatatca
     3661 agaaacaaaa gttttacaat acgtttaagc gatattaaat gaatcaaaac agctatggct
     3721 aacaaaagaa acaccttttc ttttggatag catagctgtt tttttgcaat aaaattaatt
     3781 tttattcatt ttatacttga aatacttgtg taaaaagttc ataatatcca ataatatgaa
     3841 taaagttgct ttttgttaag caagagcagt tgcaaaatag aagggaatct tttgcaagtg
     3901 atttgttcaa ttttataagt gagggtgatg gaaatggaaa agaaatcgac gggtattagc
     3961 aaatggggac tggttgctct ggttgtcagt tcgtcaatcg gaagcggtgt gtttgggatc
     4021 acaagtgatc tggctagttc tgcagctcca gggccagcga tcctttcatg gataatcgtt
     4081 gggattggta ttttagcttt ggtattatcg ctgaatcatt taggagaaaa gcgtccagat
     4141 ctagatggtg gtattttcgg ttacgcggaa gcttcatttg gtaaattagg tggcttcatc
     4201 agtggttggg gttactggtt gtctgcgtgg ctgggaaatg ttgcttttgc aacgatgctg
     4261 atgagcgcca tgggagaatt tttcccgata ttcaaaggtg gacaaaatgt tccttctatt
     4321 ttatttgcaa gtatttttat ttggggtctg actttattag taaacaatgg agttgaagga
     4381 gcgagcgtca tcaatacaat cgtgacgatc tgtaaattag tgccattatt cctattttta
     4441 atatttgtat ttatcgcatt taaagtgaat atattcactg ctgatttttg gggtaacgta
     4501 tctgataatc tagtgaatgg aacagggcag caaggaacga tctggttgca gattaaaggt
     4561 tgtttgatgg ttatgatgtg ggtctttgta gggatcgaag gcgcaagtgt tttagccaac
     4621 cgcgcgaaga aacgttcaga agcccagcaa gcatcgatca ttggtctgct ttgtttattg
     4681 tttatctata tccttgcttc gatcttgcca tacggcgtct tatctcaaga agagttagca
     4741 actatttcac aacctgctat ggcaaatatt ttaaaaggaa tcgtgggaaa ttggggagca
     4801 gcgctgatca atatcggctt gattatttcg atcattggtt cttggttgtc ttggacgatg
     4861 ctcccagcag aaacaacgat gttgatggct cgagacggtc tgttgccgaa aggttgggga
     4921 aaaacgaata aaaaaaatgc cccaacttat tcgttagttg ttactgcaat cttgactaat
     4981 ctattcttat tgactttctt agttactgat tatgcctatc aatttgccta ttctttatgt
     5041 acagcggcaa ttttgatttg ttatctactt gtcgggatct atcaagtcca gttttcttta
     5101 aaagaaggaa tcaaaaaaca agtagtgatc ggtgggattg ctgtagtttt tgaattgatg
     5161 ggcatcattt tagcaggatt ttcttatgtc cttttatgta gtattgccta tcttcctggt
     5221 ttttgcttct actggatcgc ttgtcgtgaa accaaacatc aaattacggg aaaagaaaaa
     5281 gtaatgattg ggctgatttg tgtggccgca ctgctttcaa tcgggttgct tgctgtaggc
     5341 tggatcaaga tataaggggg aaagaaacat gaaaatcgca aatttcggtg tagaagaatg
     5401 gctaaataaa tgggaaaaga aagcggtata tgatattgca caaagctcga tcgaagcatt
     5461 gacattagac gaactcgttg gcttagatgg gactagcgta agtaaatttt tcgaaaaacg
     5521 caaaaacgaa ccattggact atggctggat cgaaggatca gatgttttca aacaagaagt
     5581 cgcttctctt tatcaaaccg tcgatccgga aaacatctta caaacgaatg gagcgactgg
     5641 tgctaatctt ttagctctat acgcgttagt ggaagcagga gaccatgttg tttcgatgct
     5701 tcctacttat cagcaattat atgacatccc caaatcttta ggtgccgctg ttgactttgt
     5761 tcatttgaag gaagaagaag attggcagtt tgatttagaa cagctagaag ccttagtcaa
     5821 acctgaaaca aaaatcatct gtttgaatag tgcaaacaat cctacaggca cattgctaga
     5881 tcggaaaact ttagaaaaaa tcgcagaaat tgctcgtaca gtagatgcat atgtattgat
     5941 agatgaagta tatgcgcctt taacggataa aggtgaattc ttgtcgatcg tagatgtcta
     6001 tgaaaaaggt atagcaacca attccctatc caaaacctac tccattccag gtattcgtat
     6061 tggctggaca gctactggac ctgagttggc agaagttttt cgaaagtatc gagactacac
     6121 gatgatttgc ggcggagtcc tgtcagatga tcttgctgta catgcattga agaataaaga
     6181 aaaaatattg gcgcgaaaca aaaagatcat tacagagaac cttgtgattt taaaacaatg
     6241 ggtagccaat gaaccgaaag ttgaacttgt cgctcccaat tacgtctcta cttctttcat
     6301 taaattagcc atcgaagaag atgaccagac attttgtatc aatttattag aagaaacagg
     6361 agtactatta gtaccaggaa gtgcctttga tttaccgaag catgcccgac taggttactg
     6421 ttgtaaaaaa gaaaccttgg aaaagggatt attcctctta tctgagttct tgaaaaaaca
     6481 ggcctaaaca aaaacactgc aaggacagtc tgtgacaact gttcaagcag tgtttttcat
     6541 ttacgcttat tgtctatgag aaggagagtg ccctgaagct atggagactc ctgaagcttt
     6601 ttcttctgca gtttcttgct gatggatctc ttgtgtattg tgtcgtacaa gcaagaagtt
     6661 gaccaaaaca agaagagaag tcgaaagaaa gacgccgcga taaccaaatg cactggaaac
     6721 actggagccg atcataggcc ctactacgtt accagttgct tggaaagatt ggttataact
     6781 gaagatccgt ccagcagctt gatgaggcga atattttgtg atcaatgctt ggacagcagg
     6841 gagcagacaa gcatcagaga taccaatcaa aaaacgtaag gcagctaatt gccaaacatt
     6901 cgtgacgaat gccattggga tataaaccag aatcgcaaaa cccaaaccaa tcgcaagtat
     6961 tctttcactc ccaatcttgt cacctaagcg accaaatctt ggagcggcta tcaaagtggc
     7021 aatcccaggt atcgaagcaa tgattccgct gatcaacgta acattcccat gtccatgcag
     7081 caattggcga atgtacagac tgatgattgg actaatggaa ttgttggatg cttggatgat
     7141 catcgttgtg acgaacatac cgatgacgac gtgtggatat ttcaagtctt tgaaaatctg
     7201 tttggcagaa accatgtttt cttttttcac tgggacaaat ttttcatgga cgaaaaacag
     7261 actcaacaag aagacgaaaa ataagatgat ccctgtaatg aaaaaggtag ggcgatagcc
     7321 aaacgctgaa gcagagatcc cgccaagaag cggtccaagt aatgttcctg taacagaacc
     7381 agttgctaag gttccaagaa cttgaccact tttttctttt ggcgtcccag ttgcgacaag
     7441 ggcagtggca ttactgatat aaccagaaaa aatcccttga agcagacgca atgcgactaa
     7501 ttgatacaca ctagtcacgc aacccatcaa agaaatgacg attgccattc ctaatgaagc
     7561 acgcaacagc atcaattttc ttcctttttg gtcggctaat tttccccacc aaggagagat
     7621 aagcgtagtc accaagaaag tagatgaaaa agtcaatccg ctccaaaagt tcaattgtga
     7681 cgtactataa ttacctaacg tatctatgta aagtgacata aaaggcatga ctaaactaaa
     7741 tccgatacca gccataaatg ttccaaacca taagacaaac aagttttgtt cccaagtttc
     7801 tcttttccta aataattttt cctttattct tgcagccaat tagactcctt ctttctgaaa
     7861 attttcatct acagctcttt attataaact acaatatgta gtttatcaat gaaagtctct
     7921 tttttctctt tctttttcgt ggtaaactga aagagtgaag gatttatttt atgagaggaa
     7981 acatggaaat gaaaaaaaga aatctgtctc aagagaaaat catcgactgc tttcgagaac
     8041 tagcagaaga gatggatgtt cagcaaataa cctttcagca tttagccaaa cgcttggata
     8101 tcaaggctcc ctcgctgtat aaccatttca aaaatattcg tgatgtcaaa acagcgctaa
     8161 cagcgaaact tctgcaggaa cttaatgacc agctgcgacg ggctcttgtg ggaaaaagtg
     8221 gtcaggaagc actgcagatc tatgcagaaa cttatcgatc ctttgctttt tcaaatcaag
     8281 cagtgtatga gttactgatc agtgtaccgc ataccaatga agctgtttta ttagatggaa
     8341 tccatgaaac gaatcagatc attttgcaga tatttgaggc ttttcaatta acgaaagaag
     8401 aaaaagttca tcgaagcagg gaacttcgta gtatgatcca cggttatctt tccttacgtt
     8461 ttttaggata ctttacaaaa gaaccaacaa tcgatccaga agacagctat ttttggatga
     8521 tcaataactt tatcgctact ttgccttaaa caatcagatt catttgtaac aattttaaga
     8581 tgttcattaa atgacccttt tataagagaa aattcttgca actttcttga aaagtgacta
     8641 agatgagtct agaaaagctg aaagaaggag cacgagtatg tatcaagtaa ttaccatgtt
     8701 cggggacaat gagccttggt ggttttttga agactgggaa gaagatattg aagaagaaga
     8761 aacattcgag tctttcgaag aagcacgaca agcgtacgaa aaacaatgga acgaaatcca
     8821 tcaaaattat gaatatatca atgcaaaaag taactttctc agcgcctttt ggaatgacgg
     8881 agatgagcgc tggtgtgaag agtgtgacga tgatttacag caatacaagg gacttgcgtt
     8941 gttgaaaaat catcagccca tcacagtaga aagcagaaag gaattttatg aaacaactaa
     9001 ttctagcgga aaaaccaagc gttgcgaaag acctaagcaa ggtgcttggt gctaatcaaa
     9061 agcacaaaaa ttattatgaa ggacccaagg tgatcgttac ttgggccctt ggtcatttat
     9121 taggattgaa aatgccggaa gatctgaata aggaatggca gagctggcaa atggacacgt
     9181 tgccaatgat ccctaaaaaa ctaggaatca aaccacttcc taaaacagga catcaattaa
     9241 aagcaatcaa gcagctggct cagcgaaaag atgtctcaga agcagtcatt gcgacagatg
     9301 ccggaagaga aggggaactt gttgctcgct ggatcttaga gtatgtgcat ttcaataaac
     9361 cagtgaagcg gttatggatc tcttctcaaa cagataaagc aatcaaaact ggattcaagc
     9421 aactaagacc tgcgaaagaa tatgatgcat tgtatgaatc tgctcttgcc cgggcaaaag
     9481 cggactggct ggtaggattg aatgtgacca gagccttaac tgtgaagtac gcggataatt
     9541 tatcagcagg ccgtgtgcag acaccgacac tagcattagt gagagaccaa gaaaagaaaa
     9601 tcgagcagtt ccgtccacaa acgtatttca cgatcacgtt gcaagctgga aaagaacaag
     9661 ccaaaatgct tcaaaaaaat caatttgctt taaaaacaca agaagaagca caaaaacttg
     9721 ttcaattgat cagtacaagc aaaggaactg tcacagaagt ccaagaaaaa ttaaaaacgg
     9781 aatctgcgcc attgccttat gatttaacag agatccaaag agaagccaat caacgctatg
     9841 gctattctgc aaagaaaaca ttaagtcttg tacaaagttt gtatgagacc cataagatcg
     9901 ttacttatcc gcgtacagac agcaaatatc tgacaaatga catgaaaagt acgatgaaag
     9961 aacgattgca agcagtcagc gactttgctc ctgaggtcaa gcagtatttg aaaaacggag
    10021 ccatcgtcca acagacaaaa gtattccaag acagtaaagt gaccgatcac catgcacttt
    10081 tgccaactga aaacagagca cgttatgaaa aattaagtaa tgaagagcga aaaatctatc
    10141 aaatgattgt ctctcgtttt ttaggactgt ttgctcaacc ccataaagtc agccaaacaa
    10201 aagttacggt tgaatttggt aaagaacgat tcatttttcg gcaaaatcgt gtcgtccaag
    10261 caggatggaa aggcgaaact gaaactgaga cggaaacagt caaatgggaa aaaggaatga
    10321 gaatcaatcc cgatttcgcc atcaaaaaag aactatctgc accgcctaaa ccattgtcag
    10381 aagcaagctt gcttggtcaa atggaaaaat acagtctggg aaccccagct acgcgtgcag
    10441 agattatcga gaaactaatc aaatctgaac tgatggaacg tacccctcaa ggactgcaag
    10501 tatctcctaa aggtaagcaa ttattagtat tggtaaatcc ctctttagta acaccagatt
    10561 tgacagaaag ctgggaaaaa gaccttgaag ctattgctgc aggaaagaaa caagcaggaa
    10621 ccttcttgaa tggaatcgaa aaagaaacaa aacgcttggt gaatgagatc aaatcaagca
    10681 aacaagaata tcaagatttc tcgattaccc aaaagaaatg cccagaatgt ggggccaatc
    10741 tgcgcgagaa aaacacacgt gacggcaaaa tctatgtctg caccaattca gattgcaact
    10801 accgcagaag aaaagatcca aaagtctcta atcatcgatg cccgcaatgt caccggaaaa
    10861 tggaaatcat cgaaggtaaa aacggtgctt atttccgttg taaatacgat ggaacgacag
    10921 aaaaattgct tgataaaaaa gagcagaaga agaaaatgac caaacatgaa gaacgacgct
    10981 tgctgaaaaa atatgcacaa gcagaagaac cagaagaaag cccattagca gcagcactaa
    11041 aagcagcaat gaagaagtaa taagatatga aagaggttgt gatagaagtg gtcagcttca
    11101 agaaataaga aggaatttac gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt
    11161 tccgaagaag ctacttctgg aacaccattt attcggaaaa aaggactgtg acaagacttt
    11221 tgtcacagtc ctttttcatt taaaaaaatg tttacttcat ttgacgtata ttattggtga
    11281 ggcttcaatt catttggctt cactttttct gtttctctta tgatataaac aggacgctgt
    11341 ttcgtttcta agaagatttt cccaatatat ttcccgatga ttccaagaca taacaattgg
    11401 attccaccaa tgaacaggaa gatcgagacc atggaaggcc atcctgatgt aggatcacca
    11461 aacaacaatg cccgcaaaat aatgaacaaa attgcaatac cagaaccgac acaagacaaa
    11521 gcgccgataa atgaagcaat cgttaacggt gcatccgaga agttcacgat accatcaatc
    11581 gagtagttga ataatttcca gaaggaccaa gatgtttcgc cagccacacg ttcgcggttt
    11641 tcataagaaa tatattctgt tttaaatccg acccagctaa ataatccttt tgaaaatcgg
    11701 ttgtattctg ttaattctag gacggcatcc accatttgac gagtcatcaa gcggaaatca
    11761 cgagcgccat cgaccatttc tgtatcgctg attttattga tcaactggta gaattttttc
    11821 gcaaagaaac tgcgtatcgc aggctcgcct tcacgagtcg aacgccgggt gccgacacaa
    11881 tcgatatcag aagtagtgat ccggttgatc atctcaggaa gcagttcggg cggatcttgc
    11941 agatcaacat ccatcacggt cactaaatct cctgtagaag cttgcaatcc agcgtacaga
    12001 gccgcttctt tcccaaaatt tctggaaaat gagacaaagc ggacataagg ataagctgag
    12061 gctagcttct tgattgctgc taacgtaccg tcttttgatc cgtcgtcaat aaacaaatat
    12121 tcgaactcat gggcaccttt gaatttttca acctcatcaa aaaaaagagg aacagttttt
    12181 tcttcattaa aacatgggac aacaattgat actttcatcg tttctatcct ttcaatcgta
    12241 tgtttatata tggtttacat tcaaaattta ataaacctta aataacacct ttaaaatttg
    12301 attttacgta aaagaacagg tattcttttt gtaaactaac gtatttagtt taccatgttt
    12361 cctcggttta tcaaactttt aagaacaatt tttattagtt ggcaagtagt tttggataga
    12421 gaaaaaggag gaagttccag tctattgact aggagcaaac aaaatgaaaa tacacacacc
    12481 tatcggcttt ttatggttag atgcagatga atccggattg actgctgtat cttttcagcc
    12541 aatttcagaa agcgaatcag cgaatcagcg aattttaacg cgcacacaag aacagtttga
    12601 agaatatttt gcgggaaaaa gaaaagaatt tgatgttcct ttatctataa agagaggtac
    12661 gccttttcag caaaatgtct ggaaagcttt gtcgaccatt ccttatggag aaatccgaac
    12721 gtataaagaa gtagccgttg ctgttgatag tccgaaagct gtgcgagcga ttgggcaggc
    12781 aaatcgaacc aatccattgc caatcgttat tccttgtcat cgagtgatcg ggcaaaatgg
    12841 aaaactgaca ggatatatgg gaaacgcaat agatggggtt gaaatcaaac gccaatt
//
LOCUS       DAYYGY010000082        12776 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000082 DAYYGY010000000
VERSION     DAYYGY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.083"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1973
                     /locus_tag="ACHA52_002341"
     CDS             90..1973
                     /locus_tag="ACHA52_002341"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8941009.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2048..2332)
                     /locus_tag="ACHA52_002342"
     CDS             complement(2048..2332)
                     /locus_tag="ACHA52_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8941010.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2322..3056)
                     /locus_tag="ACHA52_002343"
     CDS             complement(2322..3056)
                     /locus_tag="ACHA52_002343"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8941011.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3185..3808
                     /locus_tag="ACHA52_002344"
     CDS             3185..3808
                     /locus_tag="ACHA52_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8941012.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4000..5121
                     /locus_tag="ACHA52_002345"
     CDS             4000..5121
                     /locus_tag="ACHA52_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8941013.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5128..8256
                     /locus_tag="ACHA52_002346"
     CDS             5128..8256
                     /locus_tag="ACHA52_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8941014.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8275..11469
                     /locus_tag="ACHA52_002347"
     CDS             8275..11469
                     /locus_tag="ACHA52_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8941015.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11615..12322)
                     /locus_tag="ACHA52_002348"
     CDS             complement(11615..12322)
                     /locus_tag="ACHA52_002348"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8941016.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4371 a         2236 c         2670 g         3499 t
ORIGIN      
        1 gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga
       61 aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc
      121 gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc
      181 accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat
      241 ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata
      301 tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg
      361 tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag
      421 ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt
      481 atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag
      541 gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa
      601 gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga
      661 tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt
      721 tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa
      781 acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac
      841 tgattctttg cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc
      901 tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt
      961 tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt
     1021 atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc
     1081 atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt
     1141 atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga
     1201 aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat
     1261 tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg
     1321 atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa
     1381 acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt
     1441 ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta
     1501 tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg
     1561 tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt
     1621 tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg
     1681 atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga
     1741 cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca
     1801 atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg
     1861 atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct
     1921 tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac
     1981 aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt
     2041 ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt
     2101 tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag
     2161 aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag
     2221 caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat
     2281 aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc
     2341 tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact
     2401 ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt
     2461 ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc
     2521 tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt
     2581 agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg
     2641 attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga
     2701 ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg
     2761 atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg
     2821 tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa
     2881 aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca
     2941 cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg
     3001 ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat
     3061 tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa
     3121 ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga
     3181 gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg
     3241 aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc
     3301 tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt
     3361 cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg
     3421 atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt
     3481 aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc
     3541 tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc
     3601 agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt
     3661 gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc
     3721 ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga
     3781 tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga
     3841 atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct
     3901 gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag
     3961 tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc
     4021 gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta
     4081 ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga
     4141 gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg
     4201 gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc
     4261 agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca
     4321 cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct
     4381 tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag
     4441 caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa
     4501 gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc
     4561 gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca
     4621 ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc
     4681 ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg
     4741 acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata
     4801 acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat
     4861 tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg
     4921 caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag
     4981 cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt
     5041 gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa
     5101 gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt
     5161 ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg
     5221 atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt
     5281 tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca
     5341 ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc
     5401 gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca
     5461 gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa
     5521 cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct
     5581 caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat
     5641 aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga
     5701 ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat
     5761 cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc
     5821 gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa
     5881 gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt
     5941 gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta
     6001 gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg
     6061 caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg
     6121 tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag
     6181 tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa
     6241 aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa
     6301 gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat
     6361 caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag
     6421 gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag
     6481 caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa
     6541 ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa
     6601 caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt
     6661 ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta
     6721 aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct
     6781 gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat
     6841 cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct
     6901 gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa
     6961 aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg
     7021 gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa
     7081 ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga
     7141 gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag
     7201 atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa
     7261 gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat
     7321 gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag
     7381 ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa
     7441 gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca
     7501 gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa
     7561 gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata
     7621 tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt
     7681 tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca
     7741 ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt
     7801 caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa
     7861 tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc
     7921 tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt
     7981 attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt
     8041 gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg
     8101 gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt
     8161 agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt
     8221 agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa
     8281 tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg
     8341 ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa
     8401 ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat
     8461 tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa
     8521 aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt
     8581 gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg
     8641 gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca
     8701 gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca
     8761 tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact
     8821 gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa
     8881 atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta
     8941 caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc
     9001 ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat
     9061 caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga
     9121 gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg
     9181 ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact
     9241 tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta
     9301 ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc
     9361 cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa
     9421 gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact
     9481 gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa
     9541 gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat
     9601 gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc
     9661 ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg
     9721 cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc
     9781 gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat
     9841 gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa
     9901 caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc
     9961 catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact
    10021 tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca
    10081 cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg
    10141 ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg
    10201 aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct
    10261 gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc
    10321 gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg
    10381 gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga tctagctaat
    10441 tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc
    10501 agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt
    10561 gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca
    10621 ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg
    10681 attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa
    10741 acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac
    10801 ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg
    10861 atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt
    10921 attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct
    10981 gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc
    11041 ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa
    11101 ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg
    11161 atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg
    11221 tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc
    11281 ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc
    11341 atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa
    11401 gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc
    11461 gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc
    11521 gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc
    11581 cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct
    11641 ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc
    11701 gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat
    11761 tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag
    11821 ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat
    11881 tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag
    11941 tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt
    12001 ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata
    12061 agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc
    12121 gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat
    12181 cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa
    12241 cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat
    12301 caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac
    12361 aaatccttga ctattgtaac agaaaaaaat gtctaaaaaa gctgataaat aaaggttttt
    12421 cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta
    12481 aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga
    12541 ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt
    12601 ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt
    12661 ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat
    12721 aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa ataaaa
//
LOCUS       DAYYGY010000083        12738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000083 DAYYGY010000000
VERSION     DAYYGY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.084"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA52_002349"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA52_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8941017.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHA52_002350"
     CDS             complement(322..891)
                     /locus_tag="ACHA52_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8941018.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHA52_002351"
     CDS             1077..1979
                     /locus_tag="ACHA52_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8941019.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA52_002352"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA52_002352"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8941020.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA52_002353"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA52_002353"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8941021.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA52_002354"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA52_002354"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8941022.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHA52_002355"
     CDS             8547..8732
                     /locus_tag="ACHA52_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8941023.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHA52_002356"
     CDS             complement(8794..9990)
                     /locus_tag="ACHA52_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8941024.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHA52_002357"
     CDS             complement(10160..11596)
                     /locus_tag="ACHA52_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8941025.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHA52_002358"
     CDS             11889..12698
                     /locus_tag="ACHA52_002358"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8941026.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattt
//
LOCUS       DAYYGY010000084        12136 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000084 DAYYGY010000000
VERSION     DAYYGY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.085"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHA52_002359"
     CDS             <1..610
                     /locus_tag="ACHA52_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8941027.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHA52_002360"
     CDS             723..1001
                     /locus_tag="ACHA52_002360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8941028.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHA52_002361"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHA52_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8941029.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHA52_002362"
     CDS             1458..2213
                     /locus_tag="ACHA52_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8941030.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHA52_002363"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHA52_002363"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8941031.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHA52_002364"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHA52_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8941032.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHA52_002365"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHA52_002365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8941033.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHA52_002366"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHA52_002366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8941034.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHA52_002367"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHA52_002367"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8941035.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHA52_002368"
     CDS             6950..7492
                     /locus_tag="ACHA52_002368"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8941036.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHA52_002369"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHA52_002369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8941037.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHA52_002370"
     CDS             9085..9987
                     /locus_tag="ACHA52_002370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8941038.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHA52_002371"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHA52_002371"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8941039.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHA52_002372"
     CDS             11430..11852
                     /locus_tag="ACHA52_002372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8941040.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAYYGY010000085        12008 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000085 DAYYGY010000000
VERSION     DAYYGY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.086"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(483..860)
                     /locus_tag="ACHA52_002373"
     CDS             complement(483..860)
                     /locus_tag="ACHA52_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941041.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(1160..2041)
                     /gene="galU"
                     /locus_tag="ACHA52_002374"
     CDS             complement(1160..2041)
                     /gene="galU"
                     /locus_tag="ACHA52_002374"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8941042.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(2084..3103)
                     /locus_tag="ACHA52_002375"
     CDS             complement(2084..3103)
                     /locus_tag="ACHA52_002375"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8941043.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3736..4740)
                     /locus_tag="ACHA52_002376"
     CDS             complement(3736..4740)
                     /locus_tag="ACHA52_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8941044.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4753..5802)
                     /locus_tag="ACHA52_002377"
     CDS             complement(4753..5802)
                     /locus_tag="ACHA52_002377"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8941045.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5819..6640)
                     /locus_tag="ACHA52_002378"
     CDS             complement(5819..6640)
                     /locus_tag="ACHA52_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8941046.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6627..7406)
                     /locus_tag="ACHA52_002379"
     CDS             complement(6627..7406)
                     /locus_tag="ACHA52_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8941047.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7423..7893)
                     /locus_tag="ACHA52_002380"
     CDS             complement(7423..7893)
                     /locus_tag="ACHA52_002380"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8941048.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7905..8324)
                     /locus_tag="ACHA52_002381"
     CDS             complement(7905..8324)
                     /locus_tag="ACHA52_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8941049.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8405..11182)
                     /locus_tag="ACHA52_002382"
     CDS             complement(8405..11182)
                     /locus_tag="ACHA52_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8941050.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11373..11597
                     /locus_tag="ACHA52_002383"
     CDS             11373..11597
                     /locus_tag="ACHA52_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941051.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3481 a         2522 c         1962 g         4043 t
ORIGIN      
        1 ggcattgaac gaatacgccc aataccttga catagcaaat aaaacaacgt gcagaccatg
       61 gaaaatatct acataggggt agaattttca ccctagatta ggtacaataa attaggtcga
      121 tgcactgcat tgactatggc aaaatgatcg gtcagggtgt atgtgtgtga ctggtcattc
      181 cctgattctt agcgccaact aagggtcagg gagccttttt ttattcagct gtttctatct
      241 gaatttattg taagtaatct cttatccaag tgcaagcaag aaaatacaaa aaaacattgg
      301 tatgatttat atatatataa ataacataaa aaaaggaatg gcttatcccg atacaacaag
      361 cattccttta aaaaagattc ttttatataa gtgaaaaaag cttttttcca ccaattaaat
      421 aaatctctca tctcaatagt ttatccatta ttttatcttt ttttgcgtat acctactaaa
      481 tattaccgtc ttcttctcgt tcttctactt tgttcgagag aatctgtact agaaaaagta
      541 gttgtatgtc taccttttct gcctatccga gactttcttt tcatatatgg gatataaatg
      601 attagataca gtacagcaaa aaataaagca aatacgcaag cttcccaact acctaacgcc
      661 aattcaacca tcaaggtagc aaccccaacg gatgctgcat accagataaa aatcagaaac
      721 gtttcttttt ttctgaccat gcgaaacatg ataaatagac taataagcaa tagtggcaac
      781 acaaatatcc aattgcttcc ttccatcagc cacttgagta tatcaagcag cgactgtgca
      841 acagcgacta attttcccat agtgaacaca cacctttttt tataattaaa aaattcgcct
      901 ttttattcta ctatgaatct tctggcttgg ctttcatttc tcttatgtaa tcattttctt
      961 ttatacaact taaaaaaaaa ttaaaccatt agcataatat tttctgaatc tattcacgaa
     1021 agtctttttc tatctttcgt tttccacgac tcaactgatc tagagtcaaa aaactaaaac
     1081 gacagttgcg caaatgacgc acaagctgcc gctttaataa caaatagcct ttcattctca
     1141 gcaggccact tatttccagt taactttctg ttcttagctt tttactcaac tggataatat
     1201 atgcacttaa tgaagtccca atttccgaat gttgaagtcc atattcaata tttgtcttca
     1261 tatatcctaa cttatcgcct acgtcgtatc tttttccttt aaactctctc gcatagaccg
     1321 cctctgtctg gttaagagtc tgaagtgcat ccgttagctg tatctcgttt cctgctcctg
     1381 gttcttgagt ttctaatatc tcaaatattt taggagtcag tagataacgt ccgataatgg
     1441 ctaaattact tggtgctttt cccatttcag gtttttctac aaaccctttt actctgcaac
     1501 gacccttttc gctatctgtt ttaggatcaa taattccgta tttagatgtt tcattttctg
     1561 gtacacgcat gacggcaaca gcggaagctt gtgtttcttc ataatccatc atcaattgtt
     1621 gagtcaatgg gatatcatct ttcatcaaat catcccccag catgacgaca aacggttcat
     1681 ttcctataaa tgcttttgct tgaagcacgg catcccccag tcctcttgga tgagattgac
     1741 ggatgaagtg aagattgact tgagtcgttt cttcaactaa agcgagtaat tctgtttttt
     1801 ccttttcccg caaattgttt tccaattcga tatttgcatc aaagtgatct tcaatgggtc
     1861 ttttttcttt tcccgttacg atcaagatgt cttcgattcc cgaatccaat gcttcttcca
     1921 caataaactg gatcgttggt ttatccacga ttggaagcat ttcttttgcc atggcttttg
     1981 ttgccggcaa aaaacgtgtt cctagcccag cggcaggaat gactgctttt ttgaccttca
     2041 cttgctgttc ctcctaaacg acgattaact attgatcatc tatttaacga agagaaaatt
     2101 cattctccat ttttccgtca cgtaacatga tttcttttgc tgcttttttg atatcttctc
     2161 cattatacaa aacattatag attgtttgag taattggcat ctcaacacct agctgttgag
     2221 ctagctctac tgctgctttt gtcgttgcca caccctcaac gaccattccc atgttttcta
     2281 gaacttcttc cagcttatgt ccctgtccta acaagttacc ggcacgccaa ttgcgagaat
     2341 gaacacttgt acaagtaacg atcaagtccc ccacaccact caaaccgata aatgtcagag
     2401 gattggcacc catcgcgaca ccaagacggc tgatttctgc caatcctcga gtcatgattg
     2461 ctgcttttgc atcatctccg aatcctaatc catggatagc gccggcacca attgcaatga
     2521 tatttttcag tgctgcacca gtttctacgc caatcacatc attatttgta taaatcctga
     2581 aataatcatt catgaacagt ttttgtacat aggctgctgc tttttgatca atacttgcag
     2641 ctgtaattgt tgtgatatca tgtactgcca cttcctcagc atggctagga ccagataata
     2701 caacaattgc ctgtcttttt tcttctggaa tttcttcagc caaaacttct gagattcgtt
     2761 tatgcgtatc ttgttctagt cctttacttg catgtataat gattggctta gtattcattt
     2821 taccaataag ttcctgtgcg actgaacgaa tcgcttttgt tggaacaaca aataaaatag
     2881 catctgcgtt atccactgct tctgccaatt ctttatatcc gataatcgat tctgggatcg
     2941 tcaaatcagg taaaaaatgc tgatttgtat gatacttatt gatttcatca atctgttcag
     3001 gtacatttcc ccaaatacgg acatcatgcc cgttttctgc caatgtttgt gctaaagcag
     3061 tgccccaaga tccaggacca agaactgcga ttttttgttt catggatatt cctccaaata
     3121 aataattgat aatttccatt atagtataca tttcttaaag ttgggactaa tttccattgt
     3181 ttttaagtgt ttgtttactt cttttatcgt ttttacttga aataatcaat gatatgttac
     3241 aatagtctac attttatttt tacactcgtt atgctttgct tgttcctaag aggaagcagt
     3301 tttgacaatg ttactaccag cattactatt aggaggttag gaaacttctc ctagtgagta
     3361 ttgatttaaa atacataaga gacagattga acatttctaa atctgtcagg tcctgcaacg
     3421 gacagattgt ctctgttgac ggttgacata ggaacttttg ttttagtcaa cattctggat
     3481 acaaaacgct atgtaaactg gcgcaactcg ttgtacttgg acttttacta cggatccatg
     3541 caacaactaa atagtactat tgcataacaa aaaatggttg gcacccacat gtcccattaa
     3601 acagattata agttgaactg tagataatgc aaaaaatgtg ttttataacg taattgatgc
     3661 caatatttta aaaaaataaa gggagcagtt atcgctgctc tctttatttt ttatattctt
     3721 tttatccagt ctatcctaat aatcaaactg acgatagtag cggcgtatct ctaatggatg
     3781 gcagttcaac tgctccacat gagcatcgat gcgttgtgtc actacatgca tcaataagaa
     3841 agacaaaaca cgtccgcgat atttttcact gataccttct actggatagt cttttgtgtc
     3901 tatcaatgta taattgctgc aaatttttgg caaaagttta gccacacgtt cagataacac
     3961 tctctgttca tcttcgccta aaaataatgt gactggggtt gtttcttcta cgatctctaa
     4021 cgtcccgtga agaaactcgg cagcatggat agatttcgat ggaagccagc tttgttcttc
     4081 ccagtaacac atggcatatg aataaacagc tccccattga ttccctgctc caataaaata
     4141 atgcatacta tcacgtcgat gtttttctgc aaatgataag ccaaatgcat ctgctttttt
     4201 ctctgcttcc actaaccctg ttggtaaata acgttcgagc tgttcatagt attctgaata
     4261 agcttcgaat tctccattca aatacatcaa tcgatctgct gccataaaaa atttgatttg
     4321 ttctgttcca ggagctggat aagtgaccac aaaatcgcat agctgagaca acttactgtt
     4381 cgctttatcg ataaatccaa tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt
     4441 cactacttct tgagttgtac cggtaacaga ggacaaaata acgatagaat cttttgttaa
     4501 tcttttgttt ccagtagtgt aatattctgc agcatgctgt acaaagacag gcaagttgct
     4561 tttcccattc atataagtga cagcttgcat cgaggaagca tacgttccac cgattcctag
     4621 ataatagaca gcgtcaaatt tttccgcata aagtttatcg atgatttctt ctacttgcgg
     4681 acgtaaagct agtgcgcctt ttattgcttt gatttgttct tcttcattga atttcagcat
     4741 gatttccacc tcttataatt tacttttcaa gactgtatca aagtcttcaa aaatcttttt
     4801 gttcaagtca atacctttag ctactgcggt tttataagcg aatagttgaa acggaacgac
     4861 aagcgctaat acagacagat attcatcaca ttctattgct aatccaagtg ttttctcgtt
     4921 tcttgctttt tttgtggtaa tggtgagagt ctctccaacg taccgactca tatatctttg
     4981 caacttttgc gaacgttcct tatttacact ttccccttca ataaagaata gcaaatgcga
     5041 agcatctgcc tccaggtatg ggccgtgcat ataagcttct aactcaaatc cttgtgacgg
     5101 aacgcggatt gtttcagtca atttcgtctc tgcttcttta gctgttcccc agtttggacc
     5161 atatccaatg gtgacgaaac gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa
     5221 ttcttccgtt ttttgaataa tcgctggtaa atggttgatt attttgcgca actgttgcat
     5281 gtagtcttgt tctatttttt tgctgatttt atttttactt attccaattc ctatccctaa
     5341 gagtagtaac tgtaaaacag tcgcagaata gccttttgta acgaagccta ctgattctac
     5401 cccggcacct aaatcaataa tttgtgcagc ttctaaagca agtggacttt gcacgtcatt
     5461 tgtgatagca acaacaggta acccttgttt ttgtatcatt tttactgctt caacagtaga
     5521 agcacttttc ccgctttgtg acacagcaat cacagtatcg attgaaggag aaagttttcc
     5581 ataattgaaa aaataaaaag gttcttgaat atcgacagta agatcaccat aagattccaa
     5641 tgctggcttc gctgctaaac aagcgttata agatgaaccc gttgctaaga tcagtaaatt
     5701 cactgttttt ttcatatgtt ctcttgtcga aaaatcattc tttttcaaga tcgtacttag
     5761 tacttccttt tcttcataga tataatcttg catcgtaaac attttctctg ctcctttttt
     5821 aaaaaatccc tatccacgaa ccaaaaattc caaagaaagc tagtccgatc aagatcgtta
     5881 atggtttcac ttctttcttc aatagataaa acactatgcc aaatgctcca agacataaga
     5941 tatttggaac gatatcatca aaaatacttt gaacagtcac agcattttct cctgaaccga
     6001 tttttaaagg aatattgatg gtgatcatcg tggcagtcat tgctccaacg accatcagtc
     6061 caataataga cgcgcctaat gtcaaacttt ccatcatacc gtttgcctgg atctttttca
     6121 aaaaacccgt tcctaagcga tatccccaac ctgttgctag atagcgaaac agaatgtgcg
     6181 gaatattgaa aatcaaaatg aacaagatag ggcctaaaat atttccttgc atggctaaag
     6241 aggtacccac acctgtggca atcaatcgca aagttcccca aaagaaagaa tctcctaacc
     6301 ctgcaagagg tcccatcaaa gatgttttga tactatcgat cgcagtcaca tcaaattcag
     6361 gatcatcctt attttcttcc tccattgcag ccgtgattcc taaaatcagt gtaacgatat
     6421 gtggggtcgt gttaaaaaac tctagatgcc gtttcaaagc tgctgccatt tcttctttcg
     6481 actgatataa ctttttcaaa atcggaatca ttgcataaac ataagccata ttcatctgac
     6541 gttcataatt ccaagaaaac tccatttgaa aagaacgcca aaatacgtga tttagctctt
     6601 ttttcgttat tttgctgttt ttgatctcag aattcttcat cttcttctac ctcccctgca
     6661 gttgtttgaa gtggtagttg acggacattc ataatattga ctaccacaac tgctaaaatc
     6721 gcaccaaaaa tagctactcc ggtcacagga atctctaagt aagaagctat agcaaaacct
     6781 aagaaaaaat atggtgcaac ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca
     6841 agagctggaa tcaaccctgt agctactgac aacccctttt gaacgaagtc aggaatcata
     6901 tttaatagat ttccgatcgt attgcttccc accatataag aaaccattac cacaacacca
     6961 agcatcaagg aaagaccgat accggcaagc aagtgcatat gctcgactcc tttataattt
     7021 ccttcttctg cataattgtc tgctttatgg ttcataattg gaatcaaaag ccctaagtaa
     7081 atatttttca atatcaatgt caacgtagca atcggcaaac ctagtaaaag cgcggtttct
     7141 gtaccggctc cagctgtaat agcaaaagcc gtccctaaaa tcccaccggt cacaacatca
     7201 ggtggaatcg aagcacctac tgagaacgat ccgataaatg ctaactctaa agttgcgccc
     7261 ataatgatcc cagtttttac atcacctaac acgattcctg taaacagccc cgtcacaatc
     7321 ggacgagaaa gcaacgatgt ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt
     7381 cctaataaga ctgcttgtac aatcattgag gttcttcctt tcttacaaaa cttctcttac
     7441 atccactttt ttatctgctg gtacttgacg gatttctact tctatttgtt tgtcaattaa
     7501 tcgtttcaag cgttcctcct cttctggtaa aatatttacc gcttttccga tgctgcgagt
     7561 accaggtttt gccttgatcc ctcctaagtt gatggttttg atttctggat acgcttctgc
     7621 cagcttatat gcatcctcga tcgattccac tacgatgaat aacttatatt tatctgtcac
     7681 cccgcttttc aacgcggcaa tcgagtcatc aattgatttg atcactagtt tgactccttg
     7741 tggcttggct aatttgatcg ttgtctttcg tatctcattc gtaggtacat catcattagc
     7801 aatcaaaata cagtctgcgc ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa
     7861 taaccgatgg tcgactcttg tcaatacaat catttttctt cctcctaaaa ttcgtcttct
     7921 tctattttct gattgagccg ctcgttacaa aactggatca ttttttttga atcagttagc
     7981 acctgctgga tcaaaacagt tgtctctact tcagcgtcca accgagatac tagctcaatc
     8041 aatagtggca aattcaatcc ggcaacgaga tagacatgat cccgattgat atattgtaag
     8101 aattcgttgt tgacacttcc gccaaaaaga tccgtcagaa caatgatttc tttatctttg
     8161 tacctcataa aaagctgcct tactgtttcc gctaaatcaa aattctcatc agtatagcaa
     8221 tcgaatacag taatagaatg atttttccca tagatcagct ctaatgaatc taatatccct
     8281 gaagcaaatt ttccatggga agctaagata atttctctat ccactttttc atctcctctt
     8341 ttcacttaca catactaaaa aagcaagttg catgccaaaa ttagtggcaa caacttgctt
     8401 atgttcaaaa ttcttcttct atcgagagaa actctgtatc ttcactcaaa atatcataga
     8461 tataggcaat ctcagaattg gggatcatga cactataatg ctcttcaatg acactaaacg
     8521 cagatttaat agcagataac ccttcttttt gacactgctt ccatttctct aatccttgat
     8581 aggtttcgat tggcatttgt cggatcaatc gttcaatgag acagctcaca tggacataca
     8641 acgctaattt tttgggattg ctgatgcttc gattcaactg cacctctaat tctctcataa
     8701 aatgatcgat ttctcccatc accttctctg tatctagaat agtcacggaa tccagtactt
     8761 tttccaatga aaaattacga atgatgcgct gattgaatat ttccttctct gaagcattca
     8821 tagtagtgtc caaccattca gctagtacag acatcttttc ccccgagatg aggtcttcta
     8881 atgaaagata gtcgatattg gtgatatcag gatctgtggt gccaatcatt cctacgactt
     8941 cgtatatgga aaataatggg tcactgtttt tcttatcttt taattgctga tattcatatg
     9001 ggatgatttt caactgacaa gccgtaggta agcttttttc tagcagttca cttaagtgga
     9061 ccgccgtacc gattccagtg aagcaagtag taatgagtgc tttgggtttg ttttcctctg
     9121 gatagagcag tcgccattct ggctggatct gtcgaacagt ctcttctgct atctcttcta
     9181 atgatacatt tttttgcagg cattccccta ctgtgattgc taaaggtgtc gtcacattat
     9241 tcaaaatcaa aagcggtacg gataactgtt ttttaaaaaa ctgatggatt tctttcagag
     9301 atcccatgtc tactaatatg atcaatccat ttgaaacatc gctgttttcg caataatcaa
     9361 ggatttctga agcaatctgc tgtggtgtga catctagtgg catatcaaat gattcaaata
     9421 gatcctcttg taataaacga tttactacat ttgccatact gctagcagtt gcataaccgt
     9481 gagcagcaat catcgctttt ggcagtcgtt tttcttttga ccatttcgct ttttttagat
     9541 agatcgtcag taaaatccga tccatacttg tgtcttcaat atctaatttg atcttactta
     9601 attccagaat tcgttggaca tagtgataac tagatggata agactgagaa acttgttgtt
     9661 ctaattcttt gattagttgg ataatatcat gttcttccgg taaccatttg atagtgcttc
     9721 gctgatagag atagtaactc aaggcataga cactgtttcc atcgaatttg atttgatagg
     9781 cagtttccat ttgtcgaaag gtctctcgga tagattgtgt ccaataaacc agcagctcgt
     9841 gtttttcttg tctactggtc tcaaacagca aaaaatcaaa taattgaatc acttcttgct
     9901 ttaattttcc ttcgcaagaa tatagcagac agttattttt cttgaaatca attaggattt
     9961 gctcaaaact tttaacgatt cgctgctgtt cgggctgttg cttctccatt aattgctcta
    10021 aggtctggtc ttgcgtaata tagattcctt gctgtgctgt atttttagta ataggctgcg
    10081 caagtagttc tttttgcaaa tgatggatcg tgacatggat ttcaggctgt tcacgctgtt
    10141 cagcatatgc ttttgctgct gaaactttga cgatattttt cagctctcct atgtttccgg
    10201 ataatttctg acttgttaaa agtgacaaag ctggtccact gactgacaaa gggctcccca
    10261 tttttctttg ttcttcgaga aaaaaagagt aaatcagttc tagccgttca ttacgactac
    10321 gctgattaag tgcaggtagt tcgattcgga taggtattct tcgaatgaaa gttgttaaga
    10381 aatgactagt aatctcttca gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca
    10441 aattaggttc tcccatacga taaatcacac cttgatcaag ataggtgaac agtttttcct
    10501 gaccttctgg tgttaagcga tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt
    10561 caaaagcccc ttttttattt tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca
    10621 aattgctagt caaaagttca ggattatctg catattgtgc acaattaaaa gtgatgaatg
    10681 gtgcagaatc ttctagcaga tcatgaagca gacaatactg atagaccaaa ttaactaaat
    10741 aacttttacc tgtgccactt tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt
    10801 aaagagctgt tttgatttgt tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa
    10861 aagagaataa gtcttttggt tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag
    10921 aaaaataaaa attcgtgcgt aacttaaaaa attgtttttc aaaagcttct ttgtggaaat
    10981 aaatcactgg tcgagaattg attttgacta gtagttgctc atcatttaac tggttcaaat
    11041 aatgactgac tgtgtttctt tttaccccaa aatgatcagc taacctttgt gctgtaaaga
    11101 cctcgttcat atgttctaaa tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt
    11161 ccttcttcaa caacttattc acccctcctc catcatagaa aacggattca ttttgtcaat
    11221 aaaatcttta ataaagaatt gaaaaatata tcttattata gacagcagcc tacttatccc
    11281 ttataatttt tattctaaaa aacgtacagt tccttccagt ttttatactg gaatttcaac
    11341 atatgaaaga ataaggaggc tatgactatt caatgaaatc ggataaatat aaaaacagga
    11401 aacatacttc ctggaaaact tttcgagaaa tcatcggatc ttatctcgct gtagttgtat
    11461 ttttgccttt gctttatatg gctatgacag aacaaactcg ttttattggc gtagatacgc
    11521 aatatatagc cgaaacagga tttttattca tctttatctt gatttttgcg ttctataaat
    11581 ccttccatac aaaataagat tcacagattt caactttcct gtctgttttt agctattaca
    11641 aatccttata tgtggtataa caacaattta ttggcataat gaggaaaaac gagttatgat
    11701 gctttcagag agtaaagagg tactgttgtc accatccttt ttattgatta cgtacaccac
    11761 tcttagtttt ttcccattga ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt
    11821 tttctttttt tattttcaga tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt
    11881 gtcttcgttc catcaaacct gatagaatct gcgccaaacc atagttttct ccactattat
    11941 ttattcctct aagatagtac ccacaataaa cgaataaata tttttctcat tttcatactc
    12001 cttctttg
//
LOCUS       DAYYGY010000086        11675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000086 DAYYGY010000000
VERSION     DAYYGY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.087"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(605..1645)
                     /locus_tag="ACHA52_002384"
     CDS             complement(605..1645)
                     /locus_tag="ACHA52_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8941052.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1994..2320)
                     /locus_tag="ACHA52_002385"
     CDS             complement(1994..2320)
                     /locus_tag="ACHA52_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941053.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2307..3095)
                     /locus_tag="ACHA52_002386"
     CDS             complement(2307..3095)
                     /locus_tag="ACHA52_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8941054.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3463..3753)
                     /locus_tag="ACHA52_002387"
     CDS             complement(3463..3753)
                     /locus_tag="ACHA52_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8941055.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3889..4068)
                     /locus_tag="ACHA52_002388"
     CDS             complement(3889..4068)
                     /locus_tag="ACHA52_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941056.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4065..4415)
                     /locus_tag="ACHA52_002389"
     CDS             complement(4065..4415)
                     /locus_tag="ACHA52_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941057.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4408..5733)
                     /locus_tag="ACHA52_002390"
     CDS             complement(4408..5733)
                     /locus_tag="ACHA52_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8941058.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6184..6798
                     /locus_tag="ACHA52_002391"
     CDS             6184..6798
                     /locus_tag="ACHA52_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8941059.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6963..7787)
                     /locus_tag="ACHA52_002392"
     CDS             complement(6963..7787)
                     /locus_tag="ACHA52_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941060.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            8177..9364
                     /locus_tag="ACHA52_002393"
     CDS             8177..9364
                     /locus_tag="ACHA52_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8941061.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(9899..10156)
                     /locus_tag="ACHA52_002394"
     CDS             complement(9899..10156)
                     /locus_tag="ACHA52_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8941062.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10149..10418)
                     /locus_tag="ACHA52_002395"
     CDS             complement(10149..10418)
                     /locus_tag="ACHA52_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8941063.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10549..10728)
                     /locus_tag="ACHA52_002396"
     CDS             complement(10549..10728)
                     /locus_tag="ACHA52_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941064.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            10826..11062
                     /locus_tag="ACHA52_002397"
     CDS             10826..11062
                     /locus_tag="ACHA52_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941065.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(11109..>11675)
                     /locus_tag="ACHA52_002398"
     CDS             complement(11109..>11675)
                     /locus_tag="ACHA52_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941066.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3581 a         2118 c         1784 g         4192 t
ORIGIN      
        1 cgtaagggat tttattcttt atttaaaact ttgcaacaga accgtccgtt gcgggtaata
       61 ccgtaacact gaagggcacg cattaaggcg tcatcttaat gctttcctgt aggacactga
      121 cggagattac cagtctcccg agccttacag gggcctttct tttttttgta ttttcaatta
      181 tcacaattta tcgtccaaaa gtgcagaacg aaaatcaatt catcacaagc atatcagaca
      241 attaaattta aaacaactaa aaatatacaa aaaagtgtaa ttgaatatta gcagcactgc
      301 ctgaaaagaa gtaatatcag gcttttcagc ttggggaaat aagtgaatta cctctcttca
      361 tgaattatta taactcaaaa aagcacatat gcaaacacta gttccctatt tttttgaatg
      421 aaaaataact aaaatatcta acgattatga ctttaaaacc tacgtttgtg attgttttca
      481 taaactttta atctaaaaac tccggaataa tagctattta tttgtaacat ttgtaataaa
      541 attaaaatca taaaaaagtt ctaatttaaa attgatcatt tttatagtga tcgcttttta
      601 tcaactactc tagccaattt tccgtactaa ctgttggtaa ttcgttaaat gtttctcttt
      661 gttcgtttgc aagctttgcc gcaatagctt tttcgacaaa ccaatttttt acataaacga
      721 aaattagatt ctgcaaattg tttacttttt cagttttttg tttttgataa gctttcagca
      781 aagtcatata taatcctttg tcagcatcaa ttccataaga ttccagttct tcataaacaa
      841 caactgtttg ctctaattct tcagcttttt tctttgcatt atgaatagtt tggacggttt
      901 tttgagcgtc ttcgtaagta ctagaaaatg tttttatcaa gtttaataca ttttctggta
      961 caaatgtatt tatactgtcc tctttcatga tgtcgacgaa gttatctagt aaaacttgat
     1021 tttgtaactg atcagtttca gtgtctatta tgtgtctatt agtgtctata gaattagaat
     1081 agaatagatt gtctgcgatt ttcgcaatta cggttgagtc cttgatatga ctggattttg
     1141 ccgtatttgc gattttcgca attacggtgg tatcattgat ttctcgcggt tttgccgtat
     1201 ttgcgatttt cgcaattacg gtggtatcag tattgtcttc ctcttccatg attttaaaga
     1261 cgtcttgatt cgttacttct ggcttgccta agtacaataa attaggcatg gattcaatct
     1321 taccgtcacg tttatttact cttcctctct tctgtttcag aagacctgct ttttctaatt
     1381 cctttttgat tttagatact ttaccttcgc ggcattgtaa caatttcatt aattcttcaa
     1441 ctgtgaatat gaaaaatata ttatcatcag aatctaccca gttatttttg actgaataat
     1501 caaatcgatc tttaagtagc atataagcaa tttttgcatc atttgataga tccatgtatt
     1561 tttcgttagt aaaaaatacc ttaggcattt gataatattt ttcacgataa atttcttttg
     1621 atttgtaata attaaaatca ttcatagcca atcctctttc tgtaggatag cgttttgttc
     1681 aaaactatgt tacaatgaat taaacaaata tatctatcca ctatatattt ttctactgct
     1741 gataagtcgc caaactttgt taaagcagta gtcttttttt atcttttttt agtttacctt
     1801 aaaaaataaa ttaaagcaaa ccgctctatt tcaaggttta agctcatagt aactatatca
     1861 cgatattaca ataatattat atcacgatat cgtaatatta caatatgaag ataagcgact
     1921 tatcagcttt gatactactg atttttataa aaaaaatatc acgatattat gatatcgtga
     1981 tattgaatgt aaattaaatt ttattttgtt tctttgctag tttatatcct tcaatcaagt
     2041 attcttcagg tatggacttt aatagcgcat cttctataac gtctggttta cttttgtctg
     2101 attcagcaat agtcgtccaa tatttgagtg ctgacattag aattggattt atacgaatgc
     2161 tatctgctgt tcttacacta cttccttgat cttttatgac tttacttata tttttcttgg
     2221 ttaattcgat ttcttctact tcttttgatt taaaattctt tttattttta tttcctagta
     2281 atctacttac tgcagcttga ctgcgattat cttctgccat caaaatcacc aacttctttt
     2341 aaatattctt ctgtaaatgc tttatagtca tcttcagctg ttgagggttt attaaaattg
     2401 attagatctg atccagcagc ttgcgcttcg tcaacggtta ttgtcctacg aatataagtt
     2461 ttgaaaattt ttgcaccaat gactctagaa atttcggttg ctgcagacgt catttcttta
     2521 gaaatgttat aacgcttatt atacttagta aatagaactc ctcgtacttc aagcttgtcg
     2581 ttcgtatatt cttttaccgt ttcaatagtt tcacttaact ttattagtcc ttttaatgag
     2641 aaatacgcag cttcaactgg aatgataatt tcatcagaaa cagtaaatgc gttgactgta
     2701 agcattccta aacttggtgg acaatcaatt ataatataat catagttgtc cttgattggt
     2761 tgaagtactc ttttaagcct gtgttcactt cctacttgag taaattccct ctctgcagaa
     2821 gaaagggcta tgtcggctgg caaaacatca attcctttat agtccagaat agcatctgta
     2881 atgtcagcct ccccttttaa tacttcataa attgtaggtt ggtttgttcc atctatatcg
     2941 aaacctacat tatctgtagc atttccctga ggatcaatgt ccactaaaag tacattttta
     3001 ccgtaaacaa atttcaaaca tgctgctaaa ttaacgctag tcgtagtttt agaaactcca
     3061 cctttttgat tggctactga caatattctt gccattaaaa tatcccccat tcatcttata
     3121 ttataatata attatatcat aatattgtga tattgcaata tcgtatatgc attatattgt
     3181 aatattgtgt gattattttg ttttaaagat atatatcggt tcggatataa agattattca
     3241 tatgttattc gaaattaaaa taatcacttt ttattacttt aaaatttttt attaatgata
     3301 agaaatatgt tttgcgagca gcaactagaa taatttaaac attactaaac tgtctatatt
     3361 gttactaata attcccttct tatagtgcat tttcttagaa aaaaacaata ttacgatatc
     3421 atgatattaa gatatcgtaa tattatacaa ttgtgattga tattatatat gtttttttat
     3481 ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca ggagcttttc
     3541 ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga atgaagcagg
     3601 taatgatttt attctacgtt gtcgggttat ccagcttgca accgttcctt gtgggtaggc
     3661 atataactca caaaattgtt ctacagtgat tcctaaattc ttgataatgt aaacattgat
     3721 cgggtgcgag taaacaaatg tattgataga catggtgcgt aagcctactt tcttttatta
     3781 ttgcgtttgc aataatagtt taacacacgc attattctag tatctactcc tatttgtaaa
     3841 aacaaaaaag gattcataga gataactcta tagaatccgt aaaatacctt atatatataa
     3901 cgggaagata taaaacctaa aattatgata acctgctgca gcttctacat agaaagagaa
     3961 gcgctcaaca ccaacctcat ttatcaaacg ttggccataa ccccaaatct cttctgatag
     4021 ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc taatcgactc
     4081 catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat tttgtctttt
     4141 ccaacgtaga tccccagctc atcactacct aagatcttgc caatgatatc ttctggatag
     4201 tatccttcag cgtttcgttc ttctttttga atagatactg atctattttt tatgactgca
     4261 gcgttcaata cttccgcaat ctcttcagtc gtcatttgtt cttttgctaa attttgactg
     4321 gttcgttgtt cattgtcggc atcaatttct tcagtatgat cagataaata aaaccctgcc
     4381 cactttaaac gtccacgatc attgtaatca atcattttta attcccccat ttccgcccgc
     4441 atgaccacca acaagtgtgc ttctagtaat gcttctagcg ccttctaaca ttgaagaagc
     4501 atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt tatctaaatt
     4561 ttcttcgtca atttgctttt gtggatcact aaataaatcc agctgaagcg aatcggtgta
     4621 cacaagcttg ctataggtta ttccaacatg ccttacttct tgtccttcat aatacttatc
     4681 aaacaagaaa agacaataat tctgcaattc ctttgtatta tctgtgatag ggatcttcat
     4741 ctggtgtgaa aaccctggtc tcgtttctaa gatcgacgtg ccaatgtgta aatggacaca
     4801 acctgtttta cagttatgct gcctaatgcg tatcgctact tgttccgcca tttcttttac
     4861 caccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg aatttccata
     4921 tgatttttcc tttgtcggtt ctggtgggat tgctatatct gttcgatcga tcccattagc
     4981 atggaagtac agctgtaagc caatcacacc tagtctattc ttaatcgtat aaggattcca
     5041 gttagcaaga tctctaatgc tcataatccc catctgattg agtctttttt tcattcgaga
     5101 gccaattccc caaaagtccg tcatttcagg aatattccat actttttcag gaacgttttc
     5161 gtacgtccat tccgcaacaa atccttcatt atgctttgct tcgttgtcta aggctagttt
     5221 agccaaaagg ggattatctc cgacacctac tgtagcaatc aatcctagct cttctttaat
     5281 attctcctgg atcatttgag ccagcttctt tctccgttga cttcgtgttc cttcagtcgt
     5341 aaaaagattc agtgatcggg tcacttttaa gattgattca tcgatcgagt aaatcagtag
     5401 atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc gcttgatata
     5461 taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg gtaagtcacg
     5521 tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag aagctaatat
     5581 caatcctgaa ccagtattat cagctcgact cataacaaca agttctgttg ttaatggatc
     5641 taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga ttaaaaagta
     5701 atcatttact ggctcttttg aataatccat cataaataac actccattct ttcacattat
     5761 acaaacatat gttcgatatt gtaaagaaga aatagctaaa tacataaaaa aagaagcata
     5821 ggtatgctat caccctgctt cttcttttga tttgtatgat caatttattc tatcacttta
     5881 ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag actgcaaatt
     5941 agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata aaatccttac
     6001 aacatttgag aataagttat cagtagacac ttacaaaaaa attctcatac tatttcctcc
     6061 atcattagtt tttgtctata taaggtttac cctaaataga cactggtata acattgaaaa
     6121 atatggcttc ctcgtgtatg attagggtgt attttaaata gttgcagaaa gtaggtaaaa
     6181 gttttgagag ttgcatacat acgtgtctca tcgatcgatc aaaatgaaca acgccagatt
     6241 gaagaaatga aaaaattcgg tgcagagaga attttcatcg agaaacaatc tggcgcaacg
     6301 attactcatc gacctgtttt ccaagaagca ttagattacg tccgagatca agatattttt
     6361 attgtggaag ctattgatcg acttggaaga aattatgacg agatcatcgc ttcagtcaat
     6421 tacttgaaga agaaaaatgt gaagctgatc attactagtt taccgatcat ggctgaagca
     6481 attggcaatc ctctcctgga taaattcatt aaggatctga tcatccaaat tttggccatg
     6541 atcgctgaac aagaacggac tgaatcgaaa cgtcgccagg cacaaggaat taagatcgca
     6601 aaagcaaacg gcgtctacaa agggcggccc aaattataca gtgcagacgc aaaagatccc
     6661 caacgtcgcc ttgtttacaa aagcatcgtt gaagatctta aaaatggtgt tgcgattgct
     6721 aagattgcta aagactacaa tgttacgaga caaaccgtgt atagaataaa aaaagacagc
     6781 atggtcaatg acaaatagtt gatcatgctg cttttattat cttctcatct ttaatttcct
     6841 acatataatt actatctata aaattaagtc ttactcagaa cagataaagc ataaaattcc
     6901 aacaaaccat tttttactta actaaataaa gagtctgtta atataatctt cacttattta
     6961 cgttaatccc ctactgtatt tgttggttcc ttatctatat tttctacaaa atccttatta
     7021 tcttgtgatt tagcttcgta taattctttg tatgtcgaca cattggttat actaattcct
     7081 atagctagtt cttgagactc tacttttctc tctagctcct ttgacaaagt gatttgagat
     7141 ttatttggtt ccgttggagt caattctata gattcttcat aaaaatctct atacataaag
     7201 ctatcattaa ttttataaaa aaaattaaat gtttttagca ctgccatata acattcaaaa
     7261 cattcttcta tagttggttt acttccttga ggagaatgaa ccaatttatt acgtttttct
     7321 atgaaatttt taatttcctt tcttatttct ttcggaaact caatattaaa gtacccttta
     7381 aacatgctcc ctaataattt gtctacaggg ggagattgta aattctcaaa tattagagtt
     7441 aaatcaggtt tctcttgttg ataaaattcc tttaccccta tttcacaggc tgtaattatc
     7501 gaaataaacc tatctctagt atttgatata tttttacttc ttttaaggag tataagagaa
     7561 aaggggatac tttcgtgtcc atgcatgtaa ttaactaatt gttgagcttc agaagttata
     7621 tctctaatta tatagttaac ggaattagga agtgtcttta actctaatga aatttctatc
     7681 caattactat cttctaatct gtatatgctc gaagtataat ttgattcgtc agcaagcatc
     7741 gctacatccc aaacactgat cataaaatct tttaaacgtt caatcatttc agcaatattg
     7801 ctaaaatcag ctaatttttc tttagaataa aaaaaaacat ttatatcaga atcaataaat
     7861 tctatttttt tattactata tcgtcatcat tatcttgacc atttatgttt attgttccct
     7921 gagtggggag tacttctttc aaattcaagt taaatttata ttccattact tctcctccaa
     7981 tatataaaaa ttacttaact gtatccaaat ttaatatgta aataaatcat taatttttag
     8041 ctaaattcta tgagaagttt atttttgtga ttacgtccat ataattgtgt aaaagtgaag
     8101 catcaaaaaa gccattgatc tcagttagga gtacaatgtt ttttgcagaa aacatactcg
     8161 aataggactg atatcaatga ctcaagtaca ttttactttt gaaagcgaag aaattcaagc
     8221 aattataaac gaaagcggtg cgaatgatac cgcaaaaaca cttatgacta tcatgtttaa
     8281 tcagctgatg gaagaacaac gaaatcaata tattcaggca aatgcctatg aacgttcaga
     8341 agaacggcaa tcacaaagaa atggttacta cgatcgttcg tttacaactc gaattggtac
     8401 gttagaactt catgttccac gaaccagaga cggtaagttt agtcctacta tctttgaaag
     8461 atatcagcga agcgaaaaag ctttgatcgc tgccatgatt gaaatggtca tttctggtgt
     8521 ctccactcgc aaagtaacta aaacagttga actgctaacg gatggtgcga ctgtttctaa
     8581 atcattcgtt tcaaatctga tgaaacagtt ggatccattt gtttttgagt ggagaaaccg
     8641 gagcctagaa ggctcggaat atccattttt catgtgtgat gccctgtata tgaaagtaag
     8701 ggaaaatcat cgtattgtct caaaaggtgt atatattggt atcggcattg attctgatgg
     8761 acgacgtacg attcttggtt ttgatgttca agacggcgaa tcggaagata attgggatac
     8821 cgtttttcaa tcgttcgttc aacgaggttt gttcggtgta aaactggtca tttctgatgc
     8881 acacaaaggg ttagttaagg ctgttcgcaa gaacttttta ggtgcgagct ggcaaagatg
     8941 ccaagcccac tttttgagaa atatttttga taagctgcca aagaaagttt cttctgatgt
     9001 aaaagacgag ttgaagagta tctttaaagc ctctgaactt gaattgacac gtgaaagaaa
     9061 agagcacttc ttggaaaaat atggttgtga ttcaaaatta agtgctgctt gcgacatttt
     9121 agaaaacggc tttgaagatg ctattcaaat tttgtctttt cctgaaaata tccgccgaag
     9181 gattcgtaca accaatgtgt tggaacggtt gaacgaagag attcgccgga gagaacgagt
     9241 gattcggatt tttccaaata tcaattccat cactcggata attggaacac ttttgatgga
     9301 aaaagacact gagtggctgg cttctccacg aaaatattta gaatttaatt cgaataacat
     9361 ttagcaccat gaacctgtgc gagtctcttc tttctgagga gagacatcaa gagtcgctgc
     9421 gctactgctc ctgacctctc tcctcagaaa gaaaggcatg gcagttaggt tcacaacgca
     9481 aaacattgtg cttttgatga ggttgaactt ttacacaaga ttgtggactt gactattttt
     9541 gttgtttttg ctaattaact aaaactagta caaaaaattt aaaatagatt attaaaccta
     9601 ttgcttattc tattcagtca tatatataag cgtcatcctt tagtaactaa catcccacac
     9661 tacaatagta ttataacata tctattcaac ttcaaattgt tgaaatttaa gccatttata
     9721 tttacttttc ctcaacttta gaaatgattt tactaacctt atatccgtaa tacgcgtaac
     9781 aaacacattc attaataaca atcaacacta agctatatta gctttttaaa tgactagggt
     9841 aaataaaata aggatcaaaa acttttaagt ttctgaccct ttccttactt ccgattggtt
     9901 aatagtgatc ttttgcagaa taaataaata tcgtttcatt ttcaactcta tatatcagtc
     9961 tatgttcatc tgtaattctt ctggaccatt ttccagataa atcatgcttt aatggctcag
    10021 gttttcctaa tccagcaaag ggggaacgat cgatatcttt tattaacttg ttaatctttt
    10081 ttatattgct tttgtttcct tgctcatgcc aataaagata atcatcccaa gcatcatcag
    10141 accaagcctt aatcatcaga ttcaacctcg attaagtcat gtgttttaaa tgcacctttg
    10201 gagaattgtt catctcctcg acgaattttt tccatgacgt aattattaga aagtgttctc
    10261 aacgtttctt gcatagaatc ataatctctt tttgataata caacaactgt atcttctaca
    10321 tctttacttg ttacaataag tgtttcagca tcctcattaa cttgtttcat ataactacgt
    10381 aaattttggc ggaaatttga ataagctact gcttccattc ctttcacccc ttatttattt
    10441 ctataaaaca attgtacatt atattgtaca attaagcaaa tgattaatat tctaacttct
    10501 tataattaaa agtgctatca cttttagttt ttaggataaa aagtgacgtc actttttgag
    10561 tgctggtacc tcaaatcctg ctttttcttg ggcaatcaat aaatctaacg caatacacat
    10621 aatcctggat tcacttttgc cgtacttctg ctttagttgc tgcaattctt tttgtctctt
    10681 ttcatccaac gtaacgggta accgtttatt tcctattgca attggcatta gtactctctc
    10741 ctttcatcga aataacccta tgaaaaatat ggctgcaatc atcttgaata gtagactcac
    10801 aagggctaat acaaagcgcc aaaagatcaa acaaaatacc agtattgcca aaaatactac
    10861 gaagtttaaa tgcacagctg cagcatggcg cataagtatt tcgccaacaa ataggactaa
    10921 caatagagga atgattttat tcatatggat ctccttcttt ctcttaggtt atcgggtgtt
    10981 gcccgcttat ttcgcaccac ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa
    11041 gaaccattcc cttagttact aggttttttg tccttatttc acacattctc acttttcggt
    11101 ataaatgatt attgcctttt tttcctttca aatcgttgat atggcattcg tttacggtca
    11161 agttcatttt ggatttcttc tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa
    11221 gtcgtgtacc agtcgtcgcc tgggttatct acttcttcta catattccct caaaaatgct
    11281 tcgggtacag cgagttcata atccattccc attaacacga tagattccgt tccacacgtc
    11341 aaaaatctag taaatacaag gtaataatcg tctatcgtcg catctttgct gtttagctct
    11401 tccatttctt cttccattaa ttccatgctc tcaaaaattg aataatattc tgattctgag
    11461 taatagtgtt tcctgcaaat cgtgtagctg acaaagtttt cgaccagctc actcctttct
    11521 aggtaaacat tcatgatgta tttttctgtt tctagtcgta gaagctccgt tccttttttt
    11581 accgtttcag gacgacgttt atacacgtct aaacgattat ggctgactgt gaccttcgtt
    11641 tgactcgttt cactttccaa atacaattgt tcttc
//
LOCUS       DAYYGY010000087        11605 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000087 DAYYGY010000000
VERSION     DAYYGY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.088"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA52_002399"
     CDS             6..305
                     /locus_tag="ACHA52_002399"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8941067.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA52_002400"
     CDS             378..1739
                     /locus_tag="ACHA52_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8941068.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA52_002401"
     CDS             1761..2021
                     /locus_tag="ACHA52_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941069.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA52_002402"
     CDS             2035..2787
                     /locus_tag="ACHA52_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8941070.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA52_002403"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA52_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8941071.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA52_002404"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA52_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8941072.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA52_002405"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA52_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8941073.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA52_002406"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA52_002406"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8941074.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA52_002407"
     CDS             7161..8558
                     /locus_tag="ACHA52_002407"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8941075.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA52_002408"
     CDS             8639..9079
                     /locus_tag="ACHA52_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8941076.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA52_002409"
     CDS             9227..9553
                     /locus_tag="ACHA52_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8941077.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA52_002410"
     CDS             9554..10303
                     /locus_tag="ACHA52_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8941078.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHA52_002411"
     CDS             complement(10383..10838)
                     /locus_tag="ACHA52_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8941079.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHA52_002412"
     CDS             complement(11265..11540)
                     /locus_tag="ACHA52_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941080.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3664 a         2014 c         2357 g         3570 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaag
//
LOCUS       DAYYGY010000088        11526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000088 DAYYGY010000000
VERSION     DAYYGY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.089"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..707)
                     /locus_tag="ACHA52_002413"
     CDS             complement(222..707)
                     /locus_tag="ACHA52_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8941081.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    797..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1187..1828
                     /locus_tag="ACHA52_002414"
     CDS             1187..1828
                     /locus_tag="ACHA52_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8941082.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1847..2482
                     /locus_tag="ACHA52_002415"
     CDS             1847..2482
                     /locus_tag="ACHA52_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8941083.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2500..3315
                     /locus_tag="ACHA52_002416"
     CDS             2500..3315
                     /locus_tag="ACHA52_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8941084.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3634..5070
                     /locus_tag="ACHA52_002417"
     CDS             3634..5070
                     /locus_tag="ACHA52_002417"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8941085.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5259..7706
                     /locus_tag="ACHA52_002418"
     CDS             5259..7706
                     /locus_tag="ACHA52_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8941086.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVILLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7936..9003
                     /gene="rlmN"
                     /locus_tag="ACHA52_002419"
     CDS             7936..9003
                     /gene="rlmN"
                     /locus_tag="ACHA52_002419"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8941087.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8993..9199
                     /locus_tag="ACHA52_002420"
     CDS             8993..9199
                     /locus_tag="ACHA52_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941088.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9196..10299)
                     /locus_tag="ACHA52_002421"
     CDS             complement(9196..10299)
                     /locus_tag="ACHA52_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8941089.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10852..11400
                     /gene="aac(6')"
                     /locus_tag="ACHA52_002422"
     CDS             10852..11400
                     /gene="aac(6')"
                     /locus_tag="ACHA52_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8941090.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3826 a         1947 c         2485 g         3268 t
ORIGIN      
        1 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
       61 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
      121 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
      181 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
      241 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
      301 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
      361 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
      421 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
      481 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
      541 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
      601 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
      661 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
      721 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
      781 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
      841 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
      901 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
      961 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     1021 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     1081 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     1141 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     1201 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     1261 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     1321 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     1381 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     1441 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     1501 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     1561 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     1621 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     1681 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     1741 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcgtaa tgaaacaagt
     1801 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     1861 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     1921 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     1981 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     2041 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     2101 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     2161 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     2221 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
     2281 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     2341 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     2401 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     2461 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     2521 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     2581 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     2641 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     2701 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     2761 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     2821 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     2881 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     2941 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     3001 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     3061 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     3121 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     3181 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     3241 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     3301 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     3361 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     3421 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     3481 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     3541 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     3601 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     3661 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     3721 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     3781 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     3841 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     3901 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     3961 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     4021 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     4081 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     4141 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     4201 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     4261 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     4321 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     4381 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     4441 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     4501 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     4561 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     4621 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     4681 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     4741 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     4801 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     4861 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     4921 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     4981 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     5041 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     5101 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     5161 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     5221 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     5281 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     5341 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     5401 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     5461 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     5521 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     5581 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     5641 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     5701 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     5761 atttagcagc agtgatatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     5821 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     5881 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     5941 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     6001 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     6061 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     6121 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     6181 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     6241 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     6301 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     6361 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     6421 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     6481 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     6541 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     6601 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     6661 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     6721 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     6781 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     6841 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     6901 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     6961 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     7021 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     7081 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     7141 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     7201 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     7261 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     7321 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     7381 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     7441 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     7501 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
     7561 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
     7621 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
     7681 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
     7741 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
     7801 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
     7861 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
     7921 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
     7981 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
     8041 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
     8101 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
     8161 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
     8221 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
     8281 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
     8341 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
     8401 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
     8461 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
     8521 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
     8581 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
     8641 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
     8701 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
     8761 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
     8821 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
     8881 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
     8941 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
     9001 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
     9061 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
     9121 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
     9181 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
     9241 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
     9301 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
     9361 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
     9421 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
     9481 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
     9541 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
     9601 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
     9661 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
     9721 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
     9781 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
     9841 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
     9901 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
     9961 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    10021 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    10081 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    10141 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    10201 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    10261 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    10321 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    10381 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    10441 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    10501 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    10561 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    10621 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    10681 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    10741 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    10801 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    10861 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    10921 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    10981 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    11041 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    11101 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    11161 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    11221 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    11281 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    11341 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    11401 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    11461 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    11521 gtgtac
//
LOCUS       DAYYGY010000089        11472 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000089 DAYYGY010000000
VERSION     DAYYGY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.090"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1006)
                     /gene="nrdF"
                     /locus_tag="ACHA52_002423"
     CDS             complement(41..1006)
                     /gene="nrdF"
                     /locus_tag="ACHA52_002423"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8941091.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1056..3215)
                     /gene="nrdE"
                     /locus_tag="ACHA52_002424"
     CDS             complement(1056..3215)
                     /gene="nrdE"
                     /locus_tag="ACHA52_002424"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8941092.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3374..3598)
                     /gene="nrdH"
                     /locus_tag="ACHA52_002425"
     CDS             complement(3374..3598)
                     /gene="nrdH"
                     /locus_tag="ACHA52_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8941093.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            3999..4472
                     /locus_tag="ACHA52_002426"
     CDS             3999..4472
                     /locus_tag="ACHA52_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8941094.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4469..6601
                     /gene="feoB"
                     /locus_tag="ACHA52_002427"
     CDS             4469..6601
                     /gene="feoB"
                     /locus_tag="ACHA52_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8941095.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6603..6737
                     /locus_tag="ACHA52_002428"
     CDS             6603..6737
                     /locus_tag="ACHA52_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8941096.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6831..7475
                     /locus_tag="ACHA52_002429"
     CDS             6831..7475
                     /locus_tag="ACHA52_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8941097.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            7662..8855
                     /locus_tag="ACHA52_002430"
     CDS             7662..8855
                     /locus_tag="ACHA52_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8941098.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            8848..10575
                     /locus_tag="ACHA52_002431"
     CDS             8848..10575
                     /locus_tag="ACHA52_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8941099.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            10969..11166
                     /locus_tag="ACHA52_002432"
     CDS             10969..11166
                     /locus_tag="ACHA52_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8941100.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            11168..>11472
                     /locus_tag="ACHA52_002433"
     CDS             11168..>11472
                     /locus_tag="ACHA52_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8941101.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKS"
BASE COUNT         3636 a         1997 c         2372 g         3467 t
ORIGIN      
        1 ctttttattt ttcgaaaaat ttttgaatta aaataaatcc ttattccaat ccaatcaaat
       61 aatcgtcgtc tttcatcgct tctacatgac ctaacaaata gccgtttccg acttgtgaga
      121 agaagtcatg gttgcttgtg cctgttgaaa tcccgttcat tacgattggg ttgacatcat
      181 tggcagtatc tggaaagagt ggatccatcc ctaggttcat caatgctttg ttggcattat
      241 agcgtaggaa tgtcttgact tcttctgtcc agccgattgg atcgtataat tcttctgtat
      301 aacgttcttc gttttcgtaa agctcataaa gaagattgta catccagtct tttaacgttt
      361 cttgttctgc ttcaggtaac tcgttgaatc ctaattggaa tttgtaaccg atgtaggttc
      421 catgtacaga ttcatctcgg atgattagtt tgatgatctc tgctacgtta gccaatttgt
      481 tgtttcctaa ataataaaga ggagtataaa aacctgaata gaacaagaat gtttctaaaa
      541 agacgctggc aattttcttt tctagcggtg taccgtgttt gtagatttca ttgatacgct
      601 cagctttttt ttgcagataa ggattcgtat ttgtccagtc aaaaatttct tcgatttctt
      661 ttttcgtatt cagtgtacta aaaatagaag aataactttt ggcatgaacg gattccatga
      721 attgaatatt gttcaaaacg gcttcttcat ggggagtgcg gacatcttta cgcaactgat
      781 ccatgccact ttcagattgg acagtatcta gcagtgtcaa accgccaaat acatgtccta
      841 ctgtatcttt ttccaattga gacaatgtac gccaatcatc taggtcattt gaaagtggga
      901 tacgtgtatc tagccaaaat tgctctgtca gcttctccca agtggattta tcaatgatat
      961 cttcaatttc attccagttt attgcttcat aatatgtttc tgacatgaga atgctccttt
     1021 aattaaaata tgaataagcg aatctatcta tgatattaga tcacgcagct ttcacattga
     1081 ttgcttccga tttcttctgc atcatcagta aaggtacgta cgtaataaat ggatttgatt
     1141 cctttgtgga aagcgtaatg tcttaaaata ttcaaatcac gtgtcgtttg cttcgttgtt
     1201 gtcttccatt cataaatgcc ttctgggatt tcagaacgca tgaataaagt caaactcatg
     1261 ccttgatcga cgtgttgttg tgccgttgca taaacatcga ttactttacg catgtccata
     1321 tcataagcag aagtgtaata cggaatcgtc tcattggata gataaggcgc tgggtaataa
     1381 attttaccga ttttcttctc ttggcgttct tcgatcatgc gagtgatagg atgaatactt
     1441 gcactcgtat cgttgatata tgaaattgag ccatttggtg ctactgccaa gcgattttga
     1501 tgatacagtc catctttcat tactgcttgt ttcaaagcat tccaatcttc agcagttggg
     1561 ataaaaatgc cttcaaacaa tgctttgact ttttctgatt ttggctggaa ttctccagtt
     1621 acgtattttt cgaaataagt gccgtcagca taagctgatt tttcaaaatt atggaaaact
     1681 tgttttcttt cacgagcgat attgttgctt tcaacaagtg tccaataatt caacagcatg
     1741 aagtaaatat ttgtaaagtc taatgaatcc tctgagccat attccatttg ttcttttgca
     1801 agataagtgt gtaagcccat ggcacctaat ccaatcgtat gattcagctt atttccgttt
     1861 tggatcggtg gtacgacatc gatatcagat gcatctgtaa taaatgtcaa tgcacgagtc
     1921 atcgtacgca cagattgacc aaagttaggg ctttgcatca aattcacgat atttgtcgag
     1981 cctaagttac atgagatatc tgttcctaat acttcatatt cctgtttgcc gttaatcaca
     2041 gaaggcgttt gaacttgtaa aatctctgaa cataaattac tcatgatgat cttgccatcg
     2101 attggattct cgcggttcgc tgtatcaata ttgattacgt aaggatagcc agattcttgt
     2161 tgtaattttg agatttcatt ttcaagatca cgcgctttga ttttttgttt gcgaatgttt
     2221 ggatttgcaa ctagatgatc atactcagct gtaatattta cataagagaa tgggacacca
     2281 tattcttttt cgacactata tggactaaat aaatacatat cttcgttttt acgtgtcaat
     2341 tcataaaatt tatcaggtac gatcacgcca agagaaagag tttttacgcg aatcttttca
     2401 tctgcatttt ctttttttgc tgaaaggaaa gcaataatgt ctggatggaa aacgttcaaa
     2461 taaaccacgc cagctccttg acgctgacct aattgattag aatagctgaa actatcttcg
     2521 aataatttca tgactggaac gacaccactt gcagcaccct catatccttt gataggagcg
     2581 ccggcttcac gaagatttga taaagtaatc cctacgccgc caccaatacg ggacaattgc
     2641 aaagcagagt tgattgagcg cccgatagag ttcatatcgt cagtgacttg agtcaagaaa
     2701 caagacacca attcgccccg acgtttacgt ccagcattca agaaagaagg tgtagctggc
     2761 tgatatcgtt gatggatcat ttcgtctgca agagcaagtg ctaattcttc atccccgttc
     2821 gcaaagtaaa gcgcattgaa ggctactcga tcttcataag tttccagata agcggaaccg
     2881 tcgttggttt tcaacgcata ttgagaataa aatttgtaag cagccataaa agatttgaaa
     2941 gaaaaatgtt gctcgtccaa aaattgatat agcttttcaa taaatgatgg tgaatacaat
     3001 tgaatgaatt ctgcttctat atattcttga tcgatcaagt aattgatttt ttccataata
     3061 gatggaaaag tcatcgtatt tggtttgaca ttttcctcaa aaaaggcaac taaagcttct
     3121 ttatccttat tcaaagggat ttgaccattg actggccgat tgatttcatt attcagcttg
     3181 aaataactga catcttgtaa atttttaaga ctcatacgta cgcccaacct tcatttcgta
     3241 tttgatgatg cttgattgca aatcattgca ttgtttttaa gctttaaaaa aggatttgga
     3301 agtttgcgac aaaaactggt gctgtctcaa gcccttcaaa tccatgtatc acgttttgat
     3361 tcttttagtc atgttagctg gcaagtttac gcaactgatc aggacggaat ccgataactg
     3421 tttctgcatc agaagtaatg acaggaacac tttggaatcc ttgtgctttc aaccagtcta
     3481 ctgcttcagg ctgttcatcg atattgactt cttcaaaagc aatttgattt tctgttaaaa
     3541 accgtttgac catcttacat tgcatacaat tatttttaga aaagattttt acgttcatgg
     3601 tgagtgcctc ctctaacaca ttcttattat ctataataga tagtataaaa ctcttagtaa
     3661 aaaagtcaaa caacaaacac tacatattgt gttgtggaaa ctgtggaata ctaagttttg
     3721 tgtttttgaa aaaagagctg aaaacaagta tcgtgtggga aaatagcaaa tttaatagag
     3781 aaaaaattgt ggaaaactat gaagaaatat ttttttctca tttttatcta tcaatactaa
     3841 agattcgctc ataatatcac aagtataatt gacattctca attaccgaga gtattctatg
     3901 tgagaatgat tctcatttat ttgcgtaaca cgtaagaaaa ttagaacata taatgaatga
     3961 ttttaataca tgatttattt cacaagagag gaatcaatat gcagacctta gcgcaggcaa
     4021 agcagaattt cgtttatcaa gtcgaacgaa tcgaaacaga agaaaaaaca aaacgccatt
     4081 tgcaaaatat cgggatcgtt gccggaagta aaatcgtcgt cgtcaattta tcaggcaaca
     4141 atgccatttt actggtaaaa agtcatcgag tggcagtaag tcgggaaata ctaggtcaaa
     4201 tctttgtcaa taaaaagact gcttcctctg aaagctggac ttctcttgat caattaaaag
     4261 caggagagac tgggatcgtc gtagcaatcc acggacaagg cgcagtgaag cgacggttaa
     4321 tggatatggg attgacgcga aatgtaaaaa tagttatccg aaaactagcc cctctgggag
     4381 atccaatcga actgacagtg cgagggtatg aactgacgtt acgaaagtca gaggccgaac
     4441 taatcttgat acaaagggag gaaaaagaat gaatatcgcg ttagtcggaa atccaaacag
     4501 cgggaaaact agcttgttca atctcttgac tggttcgaat caatatgtag ggaattggcc
     4561 gggcgtaaca gtagaaaaaa aagaaggatc gttgaaaaaa aatccgaaca ttcatattca
     4621 ggacctaccc ggcatctatt ctttgtctcc ttatacacca gaagaagtga ttgcaagaga
     4681 ttatttggtt caggaacagc ctgaactgat catcaacatt attgatgcta ccaatttaga
     4741 acgaaatctt tatttaacta cgcagttatt ggatttagac attcctatga tcattggtct
     4801 gaatatgatg gatgtcatca aaaaagaagg aaaaaaaatc aatctagaaa agctttccta
     4861 tggtcttggt gtaccagttt taccaatcag cgtattgaaa aagcaaggaa tcgaaaaact
     4921 aatcgaaaag attcaggagt ctgcaaaaaa gcggccttct attcctgttc cctgtacata
     4981 tgatccacgt ttagaagcag ctttacatga aattgaagat gtactaggag aacaaagcgg
     5041 caagaaacgc tggtatgcaa tgaaatattt tgaacgagat cagaaggtaa gagaggatta
     5101 cgagatagca gtttctcagc aaaaagagat cgagcaactg atccagttaa cagagaaact
     5161 attggacgac gatagtgaga cgattctggt caatgaacgt tatgaattta ttacccaact
     5221 atgtacactc tgtgttgtgt caaatgattc tttccaatta tcgatgagtg acaaaatcga
     5281 tcagattgca acgaatcgtt ggttagcctt gccgatcttc gcatttgtga tgtggctgat
     5341 ctattatctg gctattcaaa cagtaggaac aatggggaca gattggatca atgacacatt
     5401 gtttggtaca tggttaccag aacatgttgg cagattgtta gctgaatggc aagtagcggg
     5461 atggatgcag gagctgatct tgaacggcat catcgcaggc gtgggagcag tgttaggatt
     5521 tctaccgcaa ttgattatat tgtttttatg cttgagcttt ctggaagact gcggatatat
     5581 ggcgaggatc gcttttgtca tggatcgact tttcaggaaa ttcggtcttt caggaaaatc
     5641 cttcattcct atgctgattg ctacaggatg tggagtgcca ggcgtcatgg ccagtcgcac
     5701 gattgaaaac gagcaagatc gtcgtttgac gatcatggtc acaaccttca tgccttgttc
     5761 tgcaaagttg ccgattattg ccttagtagc aggggccttt tttcctcatc aatcttgggt
     5821 agctccttct gcctattttc ttggtatgac tgcaattatt ttctctggaa tcagcttgaa
     5881 aaaaacacgt ttgtttgctg gagatacagc tccttttatc atggaattgc ccgcatatca
     5941 ttttcctcaa tggtcagcgg tcctaagaca aacctatgac cgcagcaagt cttttgttaa
     6001 aaaagcagga acgattatct ttgtttctag tatcgtgatc tggttcagct cttcttataa
     6061 tttttatgct caacctgttt ctgaagacca aagtatctta gcctttttcg ggcgaatcct
     6121 ggcagttgtt tttcagccgc ttggttgggg aaactggcaa ggaacagtcg cagcgattac
     6181 aggattggtc gctaaagaaa atattattgg cacatttggt attttgtttg gtcatgcgga
     6241 agtatccgaa aatggcagag aaatctggca agtgctacaa cacacatata ctcccgcagc
     6301 agcttattca ttgttagcct tcaatttact ttgtgcgcct tgttttgcgg caattggggc
     6361 tatccatcgg gaaatgaatg ccaagaaatg gacttggatc gccataggct atcaatgcgg
     6421 tttagcttat ttagttagtt ttgtgattta tcagttggga cacttattgg agggtggaca
     6481 ggtagcgtta gggacgtatc ttgctatttt actgatgata ggactcgttt atgttttgtt
     6541 ccgtcagcca aaatccaaga accagtttta tactatattg tcattagaag gagaggaata
     6601 ggatggcgac attcttatta agtgtgttaa tctttggaag tgcaggcgca attgtctata
     6661 ctcgtttaaa atccggtaag aattgtgatg actgtcaaac agcttgccca gtaaaaaagg
     6721 aacagatcaa gcgatgatca gtcgttgcaa gaggctattc tccccttttt tgcttatttt
     6781 gctagagcat agtaagatga gggaaagtaa aaaaagaaag aggaaagaaa atgtctggaa
     6841 agaaaaaaat cgtccgcccg cgctaccagc agattgctgt tgatttagcc gaacgtatcg
     6901 ttgaaaaccg atataaagtg ggtgagaaga tccatgcccg ctcaacactt gccagtacgt
     6961 acaatgcttc tcctgagact acccgcaagg ctatcaatgt gctggtagat cttggaatca
     7021 tggaagtacg gcatggaagt ggggcatttg ttgcttcgaa ggaaaaagca aaagatttcc
     7081 tattgcaata tcaagatgta caatccatcc aagatacaaa agcagaaatc atggcaagtg
     7141 tggagaagca acaagatgag ctgaatcatt tttctcaatt attggatcag ctggtcaatc
     7201 aaacgaagcg aatacataag atgaatcctt tacttccgtt tgaattatct ttatcagaaa
     7261 aagcaatgca tctggaccgt tcaattagcg agttgaatat ctggcaggcg actggcgcca
     7321 cagttatcgc aatttctcat aatgaagaac tgcttgtttc acctggcccc tatgctaaat
     7381 ttacggcagg ggacatcgtt cattttattg gaaatgaata tacactacag cgtatgacaa
     7441 acttttttta cccggagcat cagcttgcag aatgattgtg tgaaatatta aaaaaatatt
     7501 aacttggcta aaccttttat ttacaaagat ttagccaagt ttttttcagt ttggacaagc
     7561 aaatttcttt catttttgac aaagtgacta catgatgtta acatagtgtg gtcacttttt
     7621 tcagaaagaa aaatgaattt acataaaaaa ggagaatttg tttggtaaaa gtagaagtaa
     7681 aacatttaac aaagatattt ggtaaaaaga cacaagccgc tttagatatg atgaatgatc
     7741 accagccgaa aacggagatt ttaaagaaaa ccggtgcaac ggttggtgtg tatgacgtga
     7801 attttgacgt aaaagaagga gaaatcttcg tgatcatggg gctttccggg agtgggaaat
     7861 ccacattgat ccgtctattg aatcgtctga ttgagccaac ttcgggcagt atctatattg
     7921 atggagaaga tgtatcgaag ttaagcaaag aagaattgcg agaagtccgt cgtcataaaa
     7981 tcaatatggt tttccaaaac ttcggtctgt ttccacatcg aacgatcttg gaaaatacag
     8041 aatacgggtt agaagtccgt ggtgtaccta aagaagaacg acaagaaaaa gcagaaaaag
     8101 cattagaaaa ttctagtttg ctttcattta aagaccaata tcctagtcag ttatctggtg
     8161 ggatgcaaca acgggttggc ctagccagag ctttagcaaa tgatcctgag atcttgctga
     8221 tggacgaagc gttctcggct cttgatccgc tgatccggcg agaaatgcaa gacgaattac
     8281 tggacctgca agccaatgtt caaaaaacga ttattttcat tacccatgat ttgaatgaag
     8341 ctttacggat tggggacaga atcgccttga tgaaagacgg cgaaatcatg cagatcggaa
     8401 caggtgaaga aatattgacc aatccagcaa atgattatgt acgtgaattt gtggaagaag
     8461 tcgatcgttc caaagtgctt acggctcaaa acatcatggt acctgcattg acgacgaata
     8521 tcgaatctga tggaccaaac gttgcattga caaggatgcg taatgaagaa gtcagcatgc
     8581 tgatggctgt tgacagaaag agacacctga aagggatcat cactgcagat caagcgttag
     8641 aagctaggaa gcaaaaacgt ccattgatcg acttcctaga tgaaaatgtg acggtgattg
     8701 ggaaagacat ggtggtaagt gatattttca atattattta tgactcaccg actccacttg
     8761 cagttgtaga ggatggaaaa ttaaaaggtg tcgtcatccg aggtagtgtg attgaagcat
     8821 tagcagaaac aagcgaggtg agtgaacatg aataatctat tgaactatca attaccagtg
     8881 gcaaactggg tagaaaatat cacagaatgg tttacgacaa ctttttctgg cctattttca
     8941 tttttacaaa cgattggaca ggcagtgatg tcggggatta caaatttact gttagtgatt
     9001 ccagctcctt tgtttatctt gttattgacg gtagcagctt tctttatttc taagaaacgt
     9061 ccaggactta cgttgttcac attaatcggt ctatggttta tttataacca aggtctatgg
     9121 aatgatttga tgaatactgt cacacttgtt cttttatcta gtgtgatctc gatcatcatt
     9181 ggggtaccat tagggatctt gatggcaaaa agcagtaaag cccaaagtat tatcaaaccg
     9241 atccttgact ttatgcagac aatgccagga ttcgtttatt tgattccagc agttgcattt
     9301 ttcggtatcg ggatggttcc aggggtattt gcctcagtca tctttgctct gccaccaacc
     9361 gttcgtttta cgaatttagg gattcgacaa gttcctaaag aattagtaga agcttctgat
     9421 tcattcggga gcacaagtcg tcaaaaacta ttcaaactag aattgccttt agctaaaagt
     9481 acgatcatgg cagggatcaa tcaaacaaca atgctgtcac tttcaatggt cgttattgcc
     9541 tctatgatcg gtgcacccgg cttaggacgc ggcgtattat cagcattgca aagagcacaa
     9601 gtcgggaatg gatttgtcaa cggggttgca ttagttatct tagcaatcat cattgaccgt
     9661 tttacacagc atttgaacca gccaaacaat aaaaaagctg caggagcaac aacacagaaa
     9721 agtaaaaaac gtcaaggact gatcattggt gctgtagtag tagtgatctt aggtgcgatt
     9781 ggtataggga gtttttcatc tgccaaagaa acaaaacgta tcaacttatc ttatgttgaa
     9841 tgggatacag aagttgcttc aacaaatgta gtcggtgaag tattgaaaca aatgggctat
     9901 gatgtaacga tgacgccatt agataattca atcatgtgga aatctgtttc aaatggcgag
     9961 tcagatgcca tggtttcagc ttggctacct aaaactcatg gttcacaata tgcacaatat
    10021 aaagatcaag tcgaagacct aggtgccaat ttgacaggag caaaagttgg tttagcagtg
    10081 ccagcctaca tggatgtaaa ttcgatcgat gagttaaccg atcaggcagg taagaaaatc
    10141 ataggaatcg agccaggagc tggtgtagtt acggctgctg aaaacacgat ccaaaaatat
    10201 gacaacttga aagactggaa agtagagaca tcttcttcag gagcaatgac cgttgcacta
    10261 ggtcaagcga tcaaaaaaca cgaaccaatt gtagttacag gatggacacc tcattggatg
    10321 tttgccaaat atgatctgaa atacttagaa gatcctgaaa acggaatggg aagcgaagag
    10381 cagatccata cgatggttcg aaaaggattg aaagaagacc agccagaagc ttacaaagta
    10441 ttagacaatt tccattggtc agaaaaagat atggaaaaag ttatgttgga aatcaacaat
    10501 ggaaaagatc cacagcaagc agcaaaagac tggatcaaag agaaccaaga attagtcgaa
    10561 agctggaaaa aataaaaaga cagacacgaa ataaatggag cttccaaaat tggatcaaat
    10621 atccaaattt ggaagctcca tgttcgtctc tatctttttt gtctataaaa aaagagaatg
    10681 cgtagctcat cttcgaaacg ttcaaagacg ccgacaaact attatgaatt tatgggtgcg
    10741 agtcaaagag atcgatcgta ggtatgaaag actaatgtga ttaaagttta ttatgtacga
    10801 agtgcattct caaaaaatat taaagaatat acatagttta tatacttaaa agtgattaaa
    10861 ttcttattca ggaattattt tgctacgttt caagaaggaa ttgttttttg aaatttaatg
    10921 aatgttagaa taatagtcta aaaacattaa tttaggggtg attagattat gaaacattta
    10981 aaaattttgt ctattaaaga gacacaactt atctatgggg gtaccactca tagtggaaaa
    11041 tattatggaa atggagtgta ttgcactaaa aataaatgta cggtcgattg ggccaaggca
    11101 actacttgta ttgcaggaat gtctataggt ggttttttag gtggagcaat tccagggaag
    11161 tgctaaaatg aaaaaaaatg ctaagcaaat tgttcatgaa ttatataatg atatatctat
    11221 aagtaaagat cctaaatatt ctgatattct tgaggtttta caaaaggtat atttaaaatt
    11281 agaaaaacaa aaatatgaat tagatcccag tcctttaata aatagattgg tgaattatct
    11341 atattttact gcttatacta ataaaataag attcactgaa tatcaagagg aattaataag
    11401 aaatttgagt gaaattggaa gaactgctgg aataaatggt ttatatcgag cagattatgg
    11461 agataaatct ca
//
LOCUS       DAYYGY010000090        11441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000090 DAYYGY010000000
VERSION     DAYYGY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.091"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..2000)
                     /locus_tag="ACHA52_002434"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="ACHA52_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2439..3308)
                     /gene="scrK"
                     /locus_tag="ACHA52_002435"
     CDS             complement(2439..3308)
                     /gene="scrK"
                     /locus_tag="ACHA52_002435"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8941102.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(3328..4449)
                     /locus_tag="ACHA52_002436"
     CDS             complement(3328..4449)
                     /locus_tag="ACHA52_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8941103.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4451..4846)
                     /locus_tag="ACHA52_002437"
     CDS             complement(4451..4846)
                     /locus_tag="ACHA52_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8941104.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4865..5662)
                     /locus_tag="ACHA52_002438"
     CDS             complement(4865..5662)
                     /locus_tag="ACHA52_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8941105.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5664..6452)
                     /locus_tag="ACHA52_002439"
     CDS             complement(5664..6452)
                     /locus_tag="ACHA52_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8941106.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6466..6942)
                     /locus_tag="ACHA52_002440"
     CDS             complement(6466..6942)
                     /locus_tag="ACHA52_002440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8941107.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(7014..9671)
                     /locus_tag="ACHA52_002441"
     CDS             complement(7014..9671)
                     /locus_tag="ACHA52_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8941108.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9735..10094)
                     /locus_tag="ACHA52_002442"
                     /pseudo
     CDS             complement(9735..10094)
                     /locus_tag="ACHA52_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(10272..10592)
                     /locus_tag="ACHA52_002443"
                     /pseudo
     CDS             complement(10272..10592)
                     /locus_tag="ACHA52_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10708..>11441)
                     /locus_tag="ACHA52_002444"
     CDS             complement(10708..>11441)
                     /locus_tag="ACHA52_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941109.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT         3666 a         2248 c         1625 g         3902 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 tgggtatcat gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc
     2401 ggacttcata agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct
     2461 tttgctaaag ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg
     2521 tattcttcga tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt
     2581 actttctttt tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt
     2641 gaaagcatca gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag
     2701 aacggatctt ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt
     2761 ccagatgcca acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct
     2821 cctgaatgtc tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa
     2881 atcccatctt gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg
     2941 gataaacctt ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt
     3001 gtccaggcaa ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc
     3061 caggcagttt ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca
     3121 atcggtccaa atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa
     3181 tcaattacca aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa
     3241 acctttaaat catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag
     3301 ccatacattt cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag
     3361 tttttcgaaa aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc
     3421 ttgacgctca taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt
     3481 ttctttgaaa ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc
     3541 atcatcgcca aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg
     3601 ggtgtgatct cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc
     3661 atatgccctt tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc
     3721 tgctcctgta gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc
     3781 tccttccccc caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata
     3841 cccagtgcct ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc
     3901 acggttccat ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt
     3961 aaatgtataa aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc
     4021 tttttttcga aatggcatct caaaatagca attcatccct gatgttggat tcactacgat
     4081 tggtaaggaa ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg
     4141 actttctaca gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa
     4201 gacgaaactc ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc
     4261 agggccttcc atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga
     4321 cccttttctc ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt
     4381 tttattttct ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa
     4441 acaattcatt ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga
     4501 tttgttgttg tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca
     4561 aagcgattga cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa
     4621 tataggtaaa aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt
     4681 cgtctgtagt atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat
     4741 atgcacataa gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa
     4801 aaccctcagc tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt
     4861 tcctttacgc taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa
     4921 tcaaaatggt catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa
     4981 ttactggaat taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt
     5041 caccaaaagt gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa
     5101 ctactgttag ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt
     5161 cagtaacaat cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga
     5221 agattaccat gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca
     5281 tataagccgc aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa
     5341 taccagcaat tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta
     5401 atccttgctt ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct
     5461 gtgtattgta atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt
     5521 ataattttct taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat
     5581 aattaaaggt acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg
     5641 ttacttgata tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct
     5701 cccgcagaag cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa
     5761 gcgaatccta tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat
     5821 ccaacaagta aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata
     5881 ccaacagcag gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa
     5941 acatccaaaa cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt
     6001 gctgcagata aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat
     6061 tctcgtttat tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca
     6121 attacatcta gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct
     6181 actccaagct ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca
     6241 gatgagccac caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc
     6301 aaacctgttt ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa
     6361 tttacaaaga tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc
     6421 agcataataa tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga
     6481 ttgaatttcc tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc
     6541 caacaatttt ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg
     6601 taatgcatta tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt
     6661 atctactaaa tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc
     6721 gtatttcccc tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat
     6781 tccagcaggt ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc
     6841 atcgatcacc atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc
     6901 atgcaataaa cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat
     6961 ctttcatttt cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct
     7021 ctttttacat aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa
     7081 tgttcaacga cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc
     7141 gtttcattga aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag
     7201 caactaatgt gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca
     7261 ttttgtaaga tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaatatg
     7321 gtcaattgct ccattaaatt cgttagacta aaattcttca gaatattatt gttcaaaact
     7381 ttcagttcat tatcattaaa gtgtctattc aattgattgg agaattttct tatattcatt
     7441 cctaaaatca tatcttcaat tgaaaagaat tctacatctt ctagatgtgg atttagtgtt
     7501 ccaacaataa aaagaatatt gttctcttta cgaagctccg ataaatagtt ttcattcact
     7561 aaagagtaat agtcacacgt ttctatgaca agatcagtat cccttggaaa gctatcttca
     7621 agtatccttt ttaatttaaa cgaagttcct aatccgcttg cacaagaaca gacaatcata
     7681 tccttcttaa tatttgatga ttcataaata taatccacat tttgattttc ttttacttgt
     7741 tcaagaatct gtttaagcgg taaatctaat aatatctgat ttgcaacatc aatagccatt
     7801 cttgttgaaa cattgtttag tagtccgaat tttttttgag cgtctagttc caaatttaga
     7861 taaattttct ccagtgaacc catatcaaca agtaaaataa tttcttcata tccggtacac
     7921 tcttgtaagt atctattaat tttgtctgca atctcactac ttgaagtcat tagtggcata
     7981 tcaatggcgt cgtatatata tctctcaacg atcctattag cagtacttgc tatacaggtt
     8041 gccgttagat ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa ttctttacta
     8101 cacattaact taaataaaat aaccaaaaca tttttttgaa tttttgttaa ataaatatcc
     8161 cctatttgaa taaaacgatt gactaattga acttctcgac tacaccaact attaatatca
     8221 ttgatgaagg aatataattt atcgtccgat gttaattgga tttgataaat ccaagaattc
     8281 aaaaagatag acgttatttg aagataataa tagtcaaatt ctatttgaaa atctctctct
     8341 atttccttaa catactgtct aaatcgaatc tcttgatagg tgggatatgt taaagctgaa
     8401 ttattttgga tatcaacatt cttttgtcta aaatatctat catatttctc aaaaagctgc
     8461 tgcttatcag tgtctttctt ccacgtttga taaagatact ctataagttc gttgatatta
     8521 aaaacttcgt ctactttttc caaatcatcc atttgaatat attttaattc attctgtctt
     8581 ttcataacct tcatgccgtt gaaattcacc cattgtggta aatgatacat ctgaatataa
     8641 agttcttcca aatcatctaa gaaataaaag gcattggcac aggtcaattg aatgatattt
     8701 ttcaaattgc caacattttc actaaagcga tagtttaata aaaaatctaa taattgttga
     8761 gatatcagta ttttctttgc tagtctttct tcttctgatt taaaaaatat cttgattaac
     8821 tgttttcttt cataaagaga acgactgtct aatgttggaa gtgttagctt tacaggtatt
     8881 cttctcaaca aagttgataa tagtttatcc tcaggtggtt ctgatgttgc aaatataatc
     8941 cagcaacttg atgtgtacca gttttcatta tcacccatcc gatgaaattt cctcgtatcc
     9001 ataaactgaa aaagtttctc ctgacattca ttgctcaatg catgaatttc atctaaaaat
     9061 aagactccat tattagctaa ttctgctaaa ccctttcttc catcttcggc tcctgtatat
     9121 gcacctttta catgcccaaa aaaattggac aagaaaagtt ctggattatt ggcatattca
     9181 ctacaattaa ctgaaatgaa agatgccttc tcttctatat aattattggc caacaaatcc
     9241 ttatacacat aatttgctaa atagcttttt ccagttccag tctcgccatg aagtaaaatg
     9301 ggtaagccat ttggcggata gtagattgct gctttaattt gagcaattac atttcgcaga
     9361 cttccttgac ttccgataac cctttctatt ccaaccctaa aagagctatc ataccatttt
     9421 tccgcttcct ctgaagagct aaaaatttgt tgatttggaa ctttctccgg taaagaaaag
     9481 aagattactg gacgagtatt gatttttcca aattctccat cttttacgaa ttcattcaaa
     9541 tattgagaga caatattacg actaacattt aatttttcag atattttctg tgctgtattg
     9601 ttcccccatt tatttttaat ctcagagttc tctatgacat aattgacaat gcgttctttt
     9661 aattcttcca tcacagcacc tcctcgacac tattcgaatt ttgccgacag tatttttcca
     9721 taaatatgac ttgctaaaaa ggctttgttt agatcattca attggaacgt tgcttgtttt
     9781 gcttgatccg ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc caaaatatca
     9841 acgattgttg caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac
     9901 atcgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact
     9961 tgttccaaca aacggtcgga taaatattta tagaaaatca gccccaataa ataattttta
    10021 tattctgacg catccatttt actacggaga ttatctgccg cactaaataa cttctgattt
    10081 aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt
    10141 attttagcat actcagtttt agaaaaagca aaaaaataaa gaaacttatt cttgataaca
    10201 agctacgtga cgtatatccc taacacaaaa atagccatat cctttggttt ggatacggct
    10261 atttgatatt actcattcaa ttatcaaatc acagtaggtg tgaatctcat cgggatcggc
    10321 aatatctact tctgattagg atcaaaggtg attttctcaa taaaggtgtg atacaaagct
    10381 ttaacttgct tcttctcact ctggataaga aagcaatcca aactagtcaa tatctgttgg
    10441 acatctttga cttgaatcgg ttccccttgt tgttgaaaga tccctaagat ttcattctct
    10501 cgaatttgta actgtcttcg tttttcagtc aattcattta aacgatcttt taacatcgga
    10561 aacaattcgg gactatcttc caaagcagct tcctgttttt gctctaatag ttagattttg
    10621 cgtaccattt tcttctttta ttttccttcg attgctgatt ttaatatagt ctaaaggaaa
    10681 tttatattta ttgtattctt tataacttca agagaacttc gtttgagtat tactgtattt
    10741 ttttaattcc ataagaaaat tagttattac ctgccaccta atcagcaaat ctgaacctga
    10801 gtcaatataa ttaatattat tttccatctc caacattgta agtactgttg gtgttaatat
    10861 agggactttg tacaattgta tttttttgat tttatcatct gcacaaagga gattttttaa
    10921 atctaatcct tcttttattt caaaagttga attttttaaa tctataagag taaaaccaat
    10981 tccttcatac tgaatttctg aaaatcgaat ttttctccca gttgatagat caaggggcag
    11041 aaaacctttt attacattgt gagtgtcacc atcatttaca atgtcattag gaattttgat
    11101 tttagaattc tgtggagcac ctattggaag ataataattc attataattt tgtagtcttc
    11161 atgaggaaat aaagtacttt taggattgct aagatattct cgtaccgaat ctaagaatgg
    11221 cttatccatt gaatcttcat ccccatttaa actaaaaaac attcctacaa ttgcttttgc
    11281 aactctaagt gaattcattt tctttattga atgtgttaaa tcagacttac cattctttaa
    11341 acttgtattg ttataaagga aatttttatt gtcttctgca aatctaatat attcctcaac
    11401 atactcacta ccaaaaaaac tattacattt actacataac g
//
LOCUS       DAYYGY010000091        11404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000091 DAYYGY010000000
VERSION     DAYYGY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.092"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1123
                     /locus_tag="ACHA52_002445"
     CDS             41..1123
                     /locus_tag="ACHA52_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8941110.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            1457..2395
                     /gene="yidC"
                     /locus_tag="ACHA52_002446"
     CDS             1457..2395
                     /gene="yidC"
                     /locus_tag="ACHA52_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8941111.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(2576..2980)
                     /locus_tag="ACHA52_002447"
     CDS             complement(2576..2980)
                     /locus_tag="ACHA52_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8941112.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(2996..3805)
                     /locus_tag="ACHA52_002448"
     CDS             complement(2996..3805)
                     /locus_tag="ACHA52_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8941113.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(3792..4682)
                     /locus_tag="ACHA52_002449"
     CDS             complement(3792..4682)
                     /locus_tag="ACHA52_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8941114.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(4695..5174)
                     /locus_tag="ACHA52_002450"
     CDS             complement(4695..5174)
                     /locus_tag="ACHA52_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8941115.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(5191..6957)
                     /locus_tag="ACHA52_002451"
     CDS             complement(5191..6957)
                     /locus_tag="ACHA52_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM8941116.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(7003..7944)
                     /locus_tag="ACHA52_002452"
     CDS             complement(7003..7944)
                     /locus_tag="ACHA52_002452"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8941117.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(7941..8936)
                     /gene="lacD"
                     /locus_tag="ACHA52_002453"
     CDS             complement(7941..8936)
                     /gene="lacD"
                     /locus_tag="ACHA52_002453"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8941118.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(8929..10122)
                     /locus_tag="ACHA52_002454"
     CDS             complement(8929..10122)
                     /locus_tag="ACHA52_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8941119.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(10140..10868)
                     /locus_tag="ACHA52_002455"
     CDS             complement(10140..10868)
                     /locus_tag="ACHA52_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8941120.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         3284 a         2455 c         2008 g         3657 t
ORIGIN      
        1 aaaagattaa tttaaaaaaa taatggaaga agttttttat atgacaaaaa atttaattgt
       61 tcgcggtgcc cctcaagagt atgaaataaa agaaaacgca tgggattcac tccctgaaca
      121 cctttacaaa aggcatattg acaacgtttt gattctccat ggaaaaacat cttggaaagc
      181 agtaaaagat gtttttccag atttatcaat aacaaaaatt tttgaatatt atggtggaga
      241 atgtaccgat ccacttgctg acagttttgt ttctttatgc aaaaagaagg atttcaatgg
      301 tattattgca gtcggcggcg ggaaagtagc agatctagga aaattgattg cctataagac
      361 aaacttacca atcatcatct tacctacctt agctgcgacg tgcgcagctt atactccact
      421 tagtgtggtc tataatgaag aaggagtgat gcttcgctat gatatgttcc ctaaagcaaa
      481 tgatttagtg ttgatcgatc caaagatcat tttgcactcc cctcgcgaga tgatgattgc
      541 cggtattggt gacaccttag caaaatggta tgaatctcac gcaatcatct ctcagttaac
      601 tatcaagcct atcgaagtcc aagtagcgga atttgcagcg ttacgatgcc gcgaaatcct
      661 tttagagaaa agtgcagagg caatggaagc gatggaccag caaaaaatca atcaagcttt
      721 tatcgatgtc gtggaaacaa acattttatt ggctggtatg gttggtgggt ttggcgatga
      781 ctacggccga acagctggag ctcattccat tcacgatgcg atgaccgtac tccctgatag
      841 ccatcagcaa cttcatggaa acaaagttgc atacggtatc cttgtccagc tgatgatcga
      901 aaataaatta gcagaaatcg aacacctact gccgttttat catcacttga acttaccaac
      961 gagtttagct gatatgggtt tacatttaac agaaagtgac tatcaagccg tagctgaacg
     1021 tgcatgttta cctgatgaaa tgatccactt catgaaagaa acgatcacac cagcactggt
     1081 tgtccagaaa atgaaagagt tagaacaagt gacacaggga tagttagccc cttataccat
     1141 ggcagtcagc ttttttatgg atcggctcaa tggtaaaaaa aaagaagtgg aaaaaattga
     1201 cattccactt cttttttttg cgctaaaatt atgatttcaa ggaaatatcc gttgctcatc
     1261 ttattttaga gaacggaggt gctaagtatg ctttatactg aagtattttt agctggattc
     1321 ttctttttaa cattaagcgt cttttttgat cctccaatcg gttatttcta tgaaatttct
     1381 atgaagttga ttgaattatg agcgcttttt tagtatagtt aagcttgtgt aaaaattata
     1441 aaagaggaag tattttatga ataaatggaa aaattggtta ttgggctctg ggcttgtcac
     1501 gatcatgctt ttcttgtccg gttgtgtccg catggattca aatggtaacc ctgacacttc
     1561 aggaatcatt taccgtatat tggttcaccc aatgggacaa gcgattacat atttagtgga
     1621 aaactttaat tggtcctacg gctgggctgt tatcttgatg acagtcatcg ttcgtatcat
     1681 cattttgcca ttaggtatta gtcaatcgaa aaaaacaatg atccaatctg aaaagatgca
     1741 agcattgaaa ccacaagttg aagctacgca acaaaaattg aaagctgcga caactcgaga
     1801 agaacaaatg gctgcacaag ctgaaatgca acaagtttat cgtgaaaatg gcttaagtat
     1861 gactggtggt atcggttgtt taccgcttct gatccaaatg ccgatctttt ctgcgctata
     1921 tttcactgct cgctatacag aaggcatccg tgaatcaacc ttttacggga tcgacctagg
     1981 taaccctagc ttagttttag tagcgattgc cggtattgct tatttacttc aaggctatat
     2041 ctcaactatc ggtatccctg aagaacaaaa gaaaacgatg cgtacgatgt tgatcgtatc
     2101 acccgctatg atcgtcttca tgtcgatcag tgccccagct ggtgtcactc tttactgggt
     2161 agtcggtggg atctttagtt gtctacaaac attcattacg aatgtcatca tgaaaccaag
     2221 attaaaagcg caagttgcgg aagaattaaa acaaaatccg ccaaaacaag tggtgacgcc
     2281 tcgtaaagat gtcacacctg ctgaacctgc gaagcaaaca aaaaaacaag tacaagcacc
     2341 aaaaaacaat gcgaagaatg gtcggaatgc tggtaaacag caaaagcgta aatagttatt
     2401 tgtaactagc ttcttcgatc gacccagatc atttctgatt ttatgacgat aaaagccaac
     2461 gtactgtgaa atatagaaaa ctatatttcg cggtacgttg gcttttttat acacggcctc
     2521 tttatacatc cgtatttata tccagttgta tcaccagtca agaagttgtg cttgattatt
     2581 cttcttcatc gtctcccatg ccagcaatca cttggttcac tgaaacgata ctctcacgac
     2641 ccgaaccaat aaaatctggt tctgctccaa tcatttgcga attgatgaat tcgatgacca
     2701 tcggcaaatt catgccagca tatagttcga tcgtttcacc ttccatgatt agcttggaaa
     2761 cggtatttgc aggtgtccca cccaacagat cgcaaagcac tacgtaatct ttcaatggtg
     2821 ctgtaacgga gaggaatctt tctttatacg tgtccgcgcc atcttcaggc aataaaggga
     2881 tcgtatagat attttcttgt ggtcccatga tcatttctgt actgtttttt agttcttcac
     2941 aaaatttacc atgactaatc aagactagtt gtctactcat ctttccaatt ctcccttatt
     3001 tcgaaatcac acctaatgca gataatgcta cacatacgat caatacgatg aatattgctc
     3061 gagtaggtgt catatttttc ttacctaaca tccaataaac gccaccaacg attccagctg
     3121 gtaaaagttt aggtaagatc atatcgatat agttttgcat attcaacgtt acatccccaa
     3181 tcgttggtgc ccatgagaat ttaacattga tcatggttgc tactaacgct ccaaccatga
     3241 aaactcctag taaagtagcc gcatctgtca atgcagtcaa gcgtgattgc atcgtggtaa
     3301 ctagagaaat cccttctttg taggcaaatt ctagttgttt ccaacgaaag accatgacag
     3361 cgacctgagc aaggatccag ataaggatac ctactggatt accatctgtt gccatatttg
     3421 cggctaaagc tccaaatatg gtcgggatca atgccgcaaa tacagaatca ccaattgcag
     3481 cgaaggaacc catcaatcct gctttcatcc cagaaacagt ttcctttgaa gagactcctt
     3541 ctttttcttc gatagcaaga tcgatcccag taatgatcgt gttaaagaaa ttagacgtat
     3601 taaaaaattg cgtatgtgtt ttcatcattt cttttaattc tggtgtatca tcaccataga
     3661 tttttctaag ttgtggcaag atagtataca gataacctga accttgcatt ctttcatagt
     3721 tccaacctaa ttggaaagta aacaaacttc tacgattgat ctgcttaaaa tcttctttgg
     3781 tcaatttata attagatttc gtcatcttcg atttccccct catcagattg tgattgctga
     3841 cttactactg gatgttggtc attcttttga tcattcttat aactgatcgt tgccagtgct
     3901 agaccaatca atgccacagc aagcatcggt aatgaattga atgttcctgg gaaatctttg
     3961 accacgctag ccacacttgt tcctaacaat tggacattcg agaacactgt tgctaataac
     4021 gctgtgatcg taaatcctaa aatcagataa gaaatgtgtt tctttactgg taggtagcgt
     4081 agtaaaattg caaacccaac tgctggtaag actgcccctg caacagataa gccatctcct
     4141 agccatttta ggtcgccgtt taaaacagcc acaacatttt ggactaagcc accaccgaat
     4201 gctagggcca agaatacagg aaccattctg gaaagtgccc aaggtaatgc ccccatcaag
     4261 aagttccgtt caattgcttt gtaattcatt gtttccacat gttgatcgat ccgatgtgca
     4321 aaatacgtat tcgcaaaacg tgccaaaata tctaattgga tcatcaaact tgctacagga
     4381 accgcgatgg ccgcaatcgc ttgttctggg ttcatcccta gagaaatgga gaaagctgtt
     4441 gctaagatcg ttccagagtt ggcatcgatt tttgatgcac caccaaatgt ccctacccct
     4501 aaaacagtca actgcatact tccaccaatg attaaacctg cttttaaatc acccattact
     4561 aacccagcat ataatcctgc aaaaactggg gcgctcattg aagaataaat ctgtaattca
     4621 tctaaaattt gataccctgc atataatgtt aataataata tctgccacca ttgaatatcc
     4681 atgaatcatt cctcctattt gattaaaaga ctcataaagt cttctgcttt gtcatttggc
     4741 accatttgag cgatcaaatg aacgccaagc tgatctaatt tcttgaagac ctctacatcc
     4801 gcatctacga cattgattga ttttgtgatg gaacgtgttt catctgtttg tgacatattg
     4861 ccgacattta attctttgat cggtacacct ttttctacta gcgctaagat acgatctggt
     4921 tttttcgcta cgatcagcac tcgttgagaa tcatatttac ctgctaaaat atttgcggct
     4981 gctttttcaa taggtaaaat acttaatttg actccagctg gcgttgccaa ctttaaccca
     5041 cttttttcga tcgcatttgt tgctacctca tcgtctacga ccatgatccg accaatattc
     5101 aactttgtcg tccaaagatt tgccacttgt ccatgtatca aacgtccatc aattcttact
     5161 ccaataattg ccatcatcca ttctcctttt ctaacttttt tctattggtt ctttgacaaa
     5221 atctataact tcactgaatc taccttctgt ttcaaagata acgatttcat tttgtccttt
     5281 tctaaataaa ccctcgggaa caaacagtga aagaattggt cctacttccc aaaaacgacc
     5341 aatattcgtg ccattcaccc aaacgatccc tttcccaaac tcactaaggt ccaaataagt
     5401 atcaccgatt tctgacagtt ccgcttcaaa gcggtaaaat gctggttgac ctgcttccca
     5461 tttttttgaa aaatcgatgt gttctgtagt ctctaaaggt aaacaatact gtgtccattc
     5521 tgtgataaaa tgaagatcgc tcatcactcc tgtacgtatt ccttttcttt gcgtatcagc
     5581 tagcagcttg tgtccatagt tgacccgtcc catattttca aataatagat caatttgatg
     5641 gtcttctttc tcaagtgtca atggaatggt ctcaccgatc tctgtttggt attgaatcgc
     5701 ttgcttttct tcatcgagaa aaacatggac ccgatcacgt ccgtcgatga tccgtaattt
     5761 ttcattgacc gcatcttttt tgatcgttgt tcgatacaat aggtacccag tattctgacc
     5821 caattcttcc attgtttgag gataggtcga gtggatcggc ttgctgatgg tatccagtgt
     5881 tgcgaaaaag ctgacttttt ctttcaagtt gattcctttt ttgacgattg tttcttttat
     5941 ccatggttcc atttgctgca tttctggaaa attttctgcc atcattcgtt tcagagcaaa
     6001 atatttttcc gttggatttc cctgctcatc caatggtgca tcgtaatcat aggaagtgac
     6061 ttgcggcaaa tcaatcgcgc ctctggctga acagccattc ataaacccaa aattggttcc
     6121 gccatggaac atatagagat tgatactgcc gatctgtaat gcttctttga ctgcatccgc
     6181 taattcttcc ggatctcttt taatgatcgg ctctttccaa cgattaaacc agccatccca
     6241 aaattccata cacatcaacg gccatttttt accatgtgct tcaaaaaact gggccatatc
     6301 tgcaaaattt tcttttgctt ttgagccaaa attccctgtc gtaaaaacat catcgtcaat
     6361 caaaatgcct gctcttaatg tggcttgcca aggtccatca gaagtgaata gcggtacatc
     6421 gattccacct tcctccaagt atttttttac ttggcgcagg tatgctttct cttcaccata
     6481 cgaaccgtac tcattttcta cctgcaccat taagacgttg ccaccatgag tgactagatg
     6541 aggaacgatt ttaggtagta gcgtttggta ataccgtttg acatacttca tgaagcgagg
     6601 atccgctgaa cgaatacggc actcctttgt caatagccaa gcaggcaagc caccaaattc
     6661 ccattctgca caaatataag gggctggacg tacgattgcg tataatccaa gggattgcgc
     6721 taactctaga aaatgggaaa gatccaatat tttcgaaaaa tcataggttc cttcttcttt
     6781 ttcatggaga ttccaaggca cataggtttc cacagtatta aatccaagcg ctttcaaatt
     6841 gtgcaatgac cgctcccaat tcgtcggatg cactcggaaa taatggatgg caccagataa
     6901 aattttgaaa ggttgaccgt ctaacaaaaa ctcttctttt atttcaaatc tagtcaagca
     6961 aatttcctcc tcgtttttag aaaccgcttt ctaattttat aatcaacgaa tctttctgac
     7021 gttgatttcc cccctaaaat aatcgacttt cacaggatcg atccatcctg ttttttcttg
     7081 ttgtgcattc gccatcccac aggccatcgc aaacgcaagt gtttcttcaa tcgtatagct
     7141 ctttgataga gcatgtgcta agcctgcaat agtcgaatct cctgacccca caggattcac
     7201 caccgcgatt ttaggaatcg taacctgata gaagtgtctc cctactttag cgattgctcc
     7261 ttttgcgcct agcgaaagaa cgatccaatt gattccttca aaaagcggat gatccaataa
     7321 tgattggatt tcatctaacg aagtagccga agaaattgtt tggtggacca actctcctaa
     7381 ttcttccaaa ttaggtttga tcaaaaatgg tttttccttg ccttccaaga cttcaagcaa
     7441 ggtttgacca gaagtatcta acaatactcg tgttcctttt ttattcgtta gggcaagtaa
     7501 ctctgcataa taacttttag aaaatccttg aggcagacta cccgaaatcg taaagatatc
     7561 agttgtttcc attagtcgtg caaaatgctc ggtaaattta tgttgctctt cttccgtcac
     7621 aacaggaccg ctttccaata gttctgtttg attcactcca tgtaaaatag caatcgaatc
     7681 tctcgtttcc cctttgatag gtaaaaattg atgcgttatc cctgattgat cgagttgttc
     7741 tttgatatat gctccatgct gcccgccgac taatccagta gctaaaacat catctgctaa
     7801 taaatgaagg acacgagcaa cattcaagcc ttttcctcca gccgttttgt tgaccgtttt
     7861 gacccgattg acttggtcga tccgtaattc atccaacgtg taagcgatgt caattgaagg
     7921 attcatggta actgtcgtga tcattttgct gtcaccgaat cttcttttac tttgtcaaac
     7981 caaggggtgg cgttttcatt tatcacttta ttcaacgatt cgatattttg tcgcccttgt
     8041 gttctcaacc actcaatcgc accttctcta ccattttcaa catatgcttt gactccatct
     8101 ttccaagtcg cacgtccgca taacacaccg ttgaatgttg aaccagattc atgagcaaag
     8161 atcaatgttt gttggaacaa tgcagtcgaa acccctgcac tcaagaagat aaatggtaac
     8221 ggtgttgcat cactttgttc cttgaagaat gccgctgctt cttttttact atacgcaatt
     8281 tcaccatcag aataaccttc tacatagttc atattgactg gcacttctac tttcaataca
     8341 tccactttat atttaggatc agaaaattct ttcatcatct cgatcacttt atgcggtttg
     8401 atcttcgcat attctaatga agtgctgtca ttggttttgg catcatacga aaccaattct
     8461 agataaaacg gacgcttttc acctgcacat tcactgccta aacgttcgat aaatacatgt
     8521 ttcaaatgat tgatctctgg gtcttcatct acatcataat aaagcaaaaa cttgatcgca
     8581 tcggctgttt gttcctttaa tctaagcgta gaccaatccg ctaataaatc aggcagacgt
     8641 cctggagttg tcgcatcata acccgttttt tcataggcta aaagtagtcc tgcttcctga
     8701 tgtctggctt tagcagctgg caatccatac tctggatcta gtaaaatagc agaagcataa
     8761 ggcgttaatt ctgctgatac taattctttg aattcagtga tctgttgcgc cgttggttct
     8821 tttcctaacg ctttgatcat ctttttcaaa gcaccgcgtt gatcaattgc caatgcactg
     8881 atgattcctt cttttgttga taaactcttc atagctgcaa ttttttcttc attcatcctt
     8941 cgtttcccct ttgttcacat attcatggat aatgacacct ttgaccactc gattgactgt
     9001 acctgtaggc gatggtgtat ctggtttatt cgctactttt atcgacgtat cgagcgcaat
     9061 gatttgggct gtcatgatca taggtaaggc aagatacgca tcaggtaggc tgatcgaagc
     9121 ttctaacaaa agacttgtac ctgtgaaatc gatgtttcct ggttgggcga tcgcgatgac
     9181 actcgtggta atttggtcat cttttatttc ttctagaata tctaagtcat aatttcttgt
     9241 atacggttgg ttattaacaa aaccaataac aagtgtttcg ctatttaaaa acgattttgg
     9301 tccatggcgg aagcccatag aagaatcaaa gatcgttgcc actttaccgg ctgtcaattc
     9361 caatattttc aattgtgctt ctctcgttgc accagccaaa cttcctgaac caagataggc
     9421 gatacgttca aatgacttag gcaataattt ttggatttct tgatctcgtg aaattagatc
     9481 ttctccagtt ctgatcaatt gttgaagata actttctttt ttctcaacgg cagtttggtc
     9541 gaatacccat aacgcactca acaacatgca cgtaaaactt cctgtcatgg caaatcctgc
     9601 atcatttgat ttttctggca tcagtaaaag ataatttcgt tcatctgtcg ctgcattctt
     9661 tgctaactga ccttctttag cacaagtaat cgtcaaatga aacaatgtct tgatttgttc
     9721 attggcaatc ttgactgttg ccacactttc tggactattt ccactacgtg caaatgacac
     9781 cagtaatgtc ggttcatccg gaaatagaaa ttcttttggt gaagacacga tatctgtcgt
     9841 accaaagcta tgaaaaataa agtgatcgtt atcaccgtat tcatttaaat aaggtactgc
     9901 tgtatccccg acatattgtg atgtgcctgc accagtaaaa atcactcgaa ctcttttgcc
     9961 atttgctgct ttttttatct ggtcaaaaaa caatgctaat ttttcttgat tctgtgctaa
    10021 tatctcagct gtttctttcc ataattgcgg ttgctgtttg atttctcgcg tagttatttc
    10081 cgcacccaac tcttttaatt tatccttcga tagttcaaac atgttgtttt ccctctcaat
    10141 cattttcgga cgtggcggat cttataatga aactggtctg ctctcgctac actatccgta
    10201 tattcaataa tttcattttt cacattatag gtcgttcggc ttagatataa taccggggca
    10261 ccatcagcga tacgaagcgg atcagcatct ttcttttgaa cgatacttgc ataaaattct
    10321 tcatctgcta attggacttg ctgttcataa tcctgagaaa acacatcata caatggctta
    10381 ctttcaagta atggcagatc aagaccaata aatagttttg caggtaaata agaacgttcc
    10441 accatcaatg gttcgtcatc cgctaaacga agtcttttca atttgaaaac tgtttcgcct
    10501 aaattgatgt ttagattctt ggcaaccttt ttgtttgctt ccatttgctt gaattccaag
    10561 atgactgtat taggcacccg tcccaattcc ctcatctctt ctgtaaaact atacgctccc
    10621 gccagatcag acgacttatc tgatagatca gacacatatg ttccttttcc gtgtcggcga
    10681 taaatatacc cttgcgtctc taattcttgc aaagcaaggc gaaccgttgt ccgactcact
    10741 ccataatgct gacaaagttc tctttcagat aacaatttgt catgaggtgt catctgtgtt
    10801 tcgatacgtt ctttcaatac gtcaactagt tgataatata atgattttcc tttacttgag
    10861 ctctccataa aaatacctct tctttactgg ttataaccac aacacaagaa tacccacata
    10921 ttcctcattt gtcaaagggt ttttggcgta aaaaagctat tataacaagg tttcaaggta
    10981 atttagaact ggtatcaaaa gatacctcaa aaataccagt tgacaaataa aaaacaaaaa
    11041 aaagctgaat cttggcaaag attcagctaa atggtctaga gaagtaaaga aaaaatattc
    11101 tttatacatc tgtggctatt tctactatcg acaagcaaat gggcctaata gactactatg
    11161 taaataaaaa tggctttctc taaataaagt aggatttttt attttttaga taacttttct
    11221 ctcatgccgg tctgatagtc gaaattttgt tgtacatttt taaaaataac aaaaattttt
    11281 tatgataatt ctgtcttttt ctagtttcct tatggcgtaa gtttaacgaa taagaattta
    11341 gtatctatac ttttatatta caaaaaatat tcgtaaaatt cgggctctat acttttcgat
    11401 gttt
//
LOCUS       DAYYGY010000092        11313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000092 DAYYGY010000000
VERSION     DAYYGY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.093"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHA52_002456"
     CDS             complement(<1..1031)
                     /locus_tag="ACHA52_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8941121.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHA52_002457"
     CDS             complement(1113..1802)
                     /locus_tag="ACHA52_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8941122.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHA52_002458"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHA52_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8941123.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHA52_002459"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHA52_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8941124.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHA52_002460"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHA52_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8941125.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHA52_002461"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHA52_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8941126.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHA52_002462"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHA52_002462"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8941127.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHA52_002463"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHA52_002463"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8941128.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHA52_002464"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHA52_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHA52_002465"
     CDS             complement(7636..8523)
                     /locus_tag="ACHA52_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8941129.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHA52_002466"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHA52_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8941130.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHA52_002467"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHA52_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8941131.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHA52_002468"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHA52_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8941132.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHA52_002469"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHA52_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8941133.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHA52_002470"
     CDS             complement(10454..10957)
                     /locus_tag="ACHA52_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8941134.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3305 a         2350 c         2069 g         3589 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ttaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaa
//
LOCUS       DAYYGY010000093        11153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000093 DAYYGY010000000
VERSION     DAYYGY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.094"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1374
                     /gene="lysA"
                     /locus_tag="ACHA52_002471"
     CDS             115..1374
                     /gene="lysA"
                     /locus_tag="ACHA52_002471"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8941135.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1361..2161
                     /gene="dapF"
                     /locus_tag="ACHA52_002472"
     CDS             1361..2161
                     /gene="dapF"
                     /locus_tag="ACHA52_002472"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8941136.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2164..3045
                     /gene="dapA"
                     /locus_tag="ACHA52_002473"
     CDS             2164..3045
                     /gene="dapA"
                     /locus_tag="ACHA52_002473"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8941137.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3042..3758
                     /gene="dapB"
                     /locus_tag="ACHA52_002474"
     CDS             3042..3758
                     /gene="dapB"
                     /locus_tag="ACHA52_002474"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8941138.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3764..4459
                     /gene="dapD"
                     /locus_tag="ACHA52_002475"
     CDS             3764..4459
                     /gene="dapD"
                     /locus_tag="ACHA52_002475"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8941139.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4475..5476
                     /locus_tag="ACHA52_002476"
     CDS             4475..5476
                     /locus_tag="ACHA52_002476"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8941140.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5473..6834
                     /locus_tag="ACHA52_002477"
     CDS             5473..6834
                     /locus_tag="ACHA52_002477"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8941141.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6794..7945
                     /locus_tag="ACHA52_002478"
     CDS             6794..7945
                     /locus_tag="ACHA52_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8941142.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7920..9089
                     /locus_tag="ACHA52_002479"
     CDS             7920..9089
                     /locus_tag="ACHA52_002479"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8941143.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9264..10643
                     /locus_tag="ACHA52_002480"
     CDS             9264..10643
                     /locus_tag="ACHA52_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8941144.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10924..11153
                     /locus_tag="ACHA52_002481"
                     /pseudo
     CDS             10924..11153
                     /locus_tag="ACHA52_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3716 a         1948 c         2496 g         2993 t
ORIGIN      
        1 aaatatacta gacaattcag aaaattttgt ttaatattat tccaataaaa ttcaaatctt
       61 tatcaaatga aattccaata aaaacggaaa aaaagtgaaa agaggaatga aaaaatgaca
      121 gaattgataa ttggcggagt cacagcaagc acattggcag aaaagtataa gacacctctg
      181 tatgtgtatg atgaagaaaa gatgagacaa acgatgctgt cattcaagcg aggctttgta
      241 tcgaaacgtt ttgatacaaa agtgctttac gcctctaaag cattccaaac gattgagatg
      301 ctgcatttag ttcgtgaata tggattcggg ctagatgttg tcagtggtgg ggaaatctat
      361 accgctttgc aagctgattt tcctagagat actatctact tccatggtaa caacaagacg
      421 ccagaggaac tgatctatgc acttgaaaat gatttgttgc atattgttgc cgataatttg
      481 atggaagttg aattattagc agaattaagc caacgctatt taaaaaaaat ctgtatcatg
      541 cttcggttga atgtcggagt agaagcacat actcatgaat atatcgtcac tgcgcatatt
      601 gattcaaaat tcggcatgag ctatgaaagt aaagagtgcc aacaagccct tcagttgata
      661 aaagaaagca gctatctaga gttagaagga tttcatgctc atatcgggtc acaaattttt
      721 gatgttactg cttggttagc tgaaatcgac aaattgacga attacttaac cgattttgac
      781 caacctcttt ccttgaatct tggcggaggg tttgggattc gttatacaca aaatgatcag
      841 ccgatcccag tcgaagaggc actaaaaaaa ctgattgaat atacggaaga agctttggaa
      901 aaacgaaaac tgaccattcg tcaattactg atcgaaccgg gtagaagtat tgttggagaa
      961 gctggtacaa cactatacac gattggtttc atcaaaaaga caccacataa aaaatattac
     1021 tttgtagatg gcgggatgac agataatatc cgtccagctc tgtatcaggc agaatacgat
     1081 tgtgatttag cagcaaaact gtcggaagaa aaaacagaga aagtgacgat tgcaggaaaa
     1141 atgtgtgaat caggcgatgt ggtaattaaa gaagcttatt tgccgaaagc agaatcgggt
     1201 gatcttttag ctgtttattc cacgggagct tatggatact cgatgagcag caactacaat
     1261 cgagcgacac gaccagcagt tgtttttgtc aataatggcc aatcaagact gattgttcgc
     1321 agacaagcat tttctgatct actaaggggg gaagtttcct atgatccttc ataaatataa
     1381 tggctgcgga aatgatttta tcttattaga ttacgcagaa gacacagatt atagtcaatt
     1441 agccgcttct ttatgcacaa aagaaaaata tgatatagac ggattgatcg ccgtaaaaat
     1501 cgatccgttg gaaatgatct attacaacaa agatggcagc cgcgcgccga tgtgcggaaa
     1561 tggcattcgc tgttttgctc gatacgtaaa tgatcagaac atgatgaatg aacaggaatt
     1621 caacgtagag acattagcag gcacaagaaa aattacggtg ttggatccct caccttttta
     1681 ttgcagcgta gatatgggag tgccagatga ttctccggaa aaaatcaaag ctaatcaaac
     1741 ttatccaata aaaaaccagg aaatagagat caatggtgaa aaagtaacga tcactagttt
     1801 atttttagga acgattcata ccgttgtttt tgttgaaaat gcgcgtgagg aagtctggag
     1861 aaaaagaggt gaactgatct gtcatcatcc tttgttcaaa gaaaaaacca atgtcaactt
     1921 tgtagaagta gtcaatgaac aagaattgat cgtccgtacc tatgaaagag gagtaggttg
     1981 gacacttgct tgcggtacag gttgctgtgc ctcttatgtg gttgctaagg aaactgggaa
     2041 aatttcagca gatcaggtag ttgttcactt ggaacaagga gacttgatga tctctggtga
     2101 ggatgcgata caaatggctg gcccagcagt tcatgaatgg acaagaatat tggaggaatg
     2161 aagatgataa aaggttcgat cgtggcgctg atcacgccga tgaatgaaga gggatcagta
     2221 gattatgctg gtttggaaaa attgatccaa ttccatttag atgaacaaac agatggatta
     2281 ttagttttag gtacaacagg agaatcttct acgctcacac aatctgagga ggagcagatc
     2341 cttcaactga cagtaaagaa agtggctggc cgtgtgccgg tgattgcagg agctggaacg
     2401 aataatacga aagaaacgat tgaaaaagcg aagcattttg cttctttggg ggcagacgca
     2461 cttttggtca tcacccctta ctacaacaaa acaagtgatg caggactagc tgctcatttt
     2521 acagcaattg cagaagcctc accgattccg cttattttgt ataatgttcc ttctagaaca
     2581 ggcatgtcga ttccaatcca tgtattagta aatttagcag aacatcctaa tatcattggt
     2641 ttgaaagaag cctcaggaga tatggcttat gtgatggatg ccgctcggtt gattggagaa
     2701 gaattcttcc tttacagtgg aaatgatgat ttgatcctac cagtaatgag tgtaggagga
     2761 agcggtgtaa taagtgtatg ggcgaatatc caacctaaaa tagtccatga attagtaaaa
     2821 gatacgcagg acggaagatg gcagcaagca aaagaaaagc aattaaatgc acttgaactg
     2881 atccatgcct tgttcagtga gaccaatcct attcctgtca aagcagccat gtcattatta
     2941 gacttacctt ccggacctct acgcctgcct ttagtttctc tttcaaaaga gaaaaagaaa
     3001 caattagctc agcttttgtt aaaagaaagg acggttgaaa aatgaaaatc ggactgattg
     3061 gcagtggaca gatgggaaat agagtcgaag aagttgcaca agaaagagga gatacggtcc
     3121 ttcttttacg gacggcacct aaagaagcaa ctacgcttgc ttttacggaa ctgccagaac
     3181 tgcttattga tttttctcat ccagataatt tagagaaaat cctttcgtat agcttatcat
     3241 atcaacttcc gttagtcatt ggcacgacag gttatacaca atcacagttc caagaaataa
     3301 aagaacatgc caaacatgta cctgtgatgt atagtgctaa tttttcgttc ggcatcatgg
     3361 tgatgaatca actaatcaag caagcagctg caatgttgca aggctggcag atcgaactga
     3421 ttgaaaaaca tcatagtcgt aaaaaagatg caccatctgg tactgcaaat atgctgcttc
     3481 aaacaatcca agaagtgcaa aagctgcagc ccgtatacaa ctggcaaggg aaaaaaagag
     3541 aagaaaacca aattggtgta cacagtattc gtgcaggttc tttacctggt gaacatgatg
     3601 tattgtttgc tgctactgat gaaatattta cgatcaaaca tgaatctttt tctaatcgaa
     3661 tctttgctgc aggcgcagta gaagcagcgg actggttgaa agatcagtca ccaggttatt
     3721 acaaattaga agatttatta atggacaaag gggtgtaaaa aacgtggaag ctcaagaaat
     3781 catccgctac attcaaacgg catctaaaaa gacgcctgtg aaagtctatc tgaatgtaac
     3841 cgagccaatc gaatttaaag aaagtaaagt ttttggtgaa ggaagcagct tcgttgtttt
     3901 tggtgactat gagacgattg ctccggtttt agaagctgaa tcagaaaaaa tcattgaaat
     3961 cgaaatcgaa actgctgcaa gatacagcgc agttcctttg ctagatatta aaaaaattaa
     4021 tgctcgtatc gaaccaggtg ctattatcag agatcaagtg tcaatcggca acaatgccgt
     4081 gatcatgatg ggagcgatta tcaatattgg tgcagtaatc ggtgaaaaca cgatgattga
     4141 tatgggtgct gtcttaggtg gacgagcgac agtgggcaaa aactgccata ttggtgcagg
     4201 agctgtctta gcaggtgtga tcgaaccagc atcggccaaa ccggttatcg tagaagacgg
     4261 cgtactggta ggtgcgaatg cagtgattgt tgaaggtgtc catatcggaa aagatgcagt
     4321 cgtggcagca ggagcagtgg ttttagaaga tgtggcagca gaaactgttg tcggtggaat
     4381 acctgcacgt gtattaaaaa taagtgatga tcagacaaaa gaaaatacag cattgattgc
     4441 tgcgttacgc gaattataga ggagatggag aaaaatgcgc gaaatagata cctgtattgt
     4501 gggggcaacc ggattagtcg gccaaacgat gctcaaagtg ttagaagaat atgattttcc
     4561 agtaggacga ttgaaattat tagcttcaaa acgttcagcc ggtaaagttt gtgtattcaa
     4621 gaaaaagaaa cacactgtac aggaattgac agaaacttca tttgaaggat atgatttagc
     4681 gctattttct gcaggtgctt ctgtatccaa agagtttgcg ccaatcgcac gaagtcaggg
     4741 actatttgtc atagataatt cctcagcatg gcgggaagat tcggatattc cattaatcgt
     4801 tccagaggtc aatctggaag actatacatt gaatcggctg attgcaaatc caaattgttc
     4861 aaccattcaa gcggtacttc cgcttaaagc tttacaagaa gcctttggta tccagcgagt
     4921 caattattcg acctatcaag cagtttctgg ttccgggcaa aaaggcattg aagatcttca
     4981 tcgtacgcaa aatgacgagg aaccagtttt gtatccttat aatattagcc aaacggtgat
     5041 tccggaaatc gatcagcctt tggaaaatgg gtatacaaaa gaagaacaga aaatgataaa
     5101 tgagacccgt aagattctgc atcagcctgc tttgccagta tcagcaacct gcgtacgtgt
     5161 gccaatcgaa aatggacatg gagtctctgt ggcagttcaa ctgcaaaaag agtttacgac
     5221 ggaagaaatt agagagtgtc tcagccagtt tccagggatt caattagaag atgatctagc
     5281 agcacataaa tatccgacga gcattcttgc tagaggaaca gatatggttt atgttgggag
     5341 gatcagaaaa gacacttctt tagagaacgg attgttgttc tatacgactg ccgataacat
     5401 acgcaaagga gccgctgcga atgccgtaca gattgctgca gctttaatgc agaaaatagc
     5461 ggaggtggca agatgagtaa tacgctgaaa gtagcgaaat tcggcggaac atcgatgtct
     5521 catgaagagc aattcaaaaa agtccgtaca attgttcttt cagatcccaa tcgccaagtg
     5581 atcgttgttt ctgctttagg caaacgtacg tcagatgatg acaaagtaac agacttactt
     5641 tatttgattt atgcttacct tcagcatcat gttgattgga cacccctttg gaaaaggatt
     5701 tgtgatcgtt tcattcaagt aagagatgtt cttgccctgc agttacctat tgaagatgaa
     5761 ctagcacgca tacagaaaca actggaagac gaaaagataa ctgaagatta tttagtaagt
     5821 agaggtgaat atctgactgc tcaactgatg gctgagtatc ttggttacca gtttattgac
     5881 gcaaaagacc tgattttctt tgactatgat gggaaaatcg atcaggaaaa aacagcacag
     5941 gaaatcacaa aagcatttaa aagatatccg aaaatggttg ttccagggtt ttacggggcg
     6001 aaccctgcag gaaaagaaaa attattgggc cgtgggggaa gcgacattac cggcgcgata
     6061 ctggcgaaga tgttgcaagc ggaaagttat gagaattgga cggatgtttc aggaatcatg
     6121 atggctgatc cgcgaatcat cgatcatcct aaaatcatcg aggaaatctc ttttagagaa
     6181 ctgagagaga tggcttatat gggggctaac gttcttcatg aagcagctgt tttcccagtt
     6241 caagaggctg atatccccat ccaaataaaa aataccaatg atcctttcgc tcaagggaca
     6301 aaaatatcga atcacgaaat agaaaaggaa aatggactga caggaatagc tggacgcaag
     6361 gattttcttt ccatcacttt attcaaacgt catatgtccg atgaaatcgg gtttatctgg
     6421 aaagcaatga gcatttttgc taaacatgag atatcaatcg aacatattcc ttcagggatc
     6481 gacaatatcg gagtgatcgt ctcagcagaa gcgattgccg ataagttatt tctcatcacg
     6541 aaagaattaa aagaaacact tggcgtggaa gaaatcgtcg tgatcgaaga cttagctttg
     6601 atctctgtcg ttgggggtcc gcataaagaa ttgattggat tatctgggaa agtgttatcg
     6661 atattgaaca aattagaaat tcgcacatcg atcttatcac aaggagcaca ggagttgaat
     6721 ctgatcatcg gtgtaccgaa taatcaatat gaaacagtag tgaagggtat ctatgaaggg
     6781 atggtgaata cggatgctga aagaatatcg aatggcactc catcaaattc ctgaaatcgg
     6841 gtttcatgaa ttcaagacaa aaaaatattt atatgaacaa gtaaaaaaca gcgaggccgt
     6901 tatccatgaa atcggcgaaa caggactact cctttatttt gataatcagc aagaagaaac
     6961 gattgctttt cgaacagata tcgatgcact gccagtcaaa gaagaaacga atctctcttt
     7021 tgcttctaag catgaaggtt atatgcatgc atgtggtcat gacggacata tggcgatgct
     7081 tttagggttg actgacttta taactaaaca cattgaggat cttatttata atgtagtact
     7141 gatctatcag ccatcagaag aaatttccgg tggcgcacaa agcgttattc agtcaggatt
     7201 gttggataaa tatgaagtac aagcaatatt tggctttcat ctatggcctg gactagctaa
     7261 gggagaagtt ttctctcgac caggaccttt gatggcacaa agcagtgaaa cagacattat
     7321 cattaaaggc cagtctgcac atattgcttc tagtgaaaaa ggaatcgata gtctggaggc
     7381 agctgtacga tttttgaaga atgtttatga ttttgaccgg caattaccgg cagagtggaa
     7441 tcatctaatg aaatttggcc aaatccaagg tggaacgatc cgaaatgttt tagctggaga
     7501 agttattgta tccggaagta tccgttctta cagttcagat atgcaaaatt atttgaagag
     7561 gggattagac catttagcag aacagtttca ctctacagct actatttctt ttcgctataa
     7621 tgacggctat ccagctgtaa tcaaccatag aggcttatat gcagcactgt tagattcaaa
     7681 caaactacat gaattagatg ctccagttct tcaagccgaa gattttggca tatatacgca
     7741 acaatatcca tgtgtatttt tctttttagg tgtcggtgat acccctgctt tacatgatcc
     7801 gacgttcgat tttgatatgg atgttttaga aaatggtgta gaatggtaca aaacgattct
     7861 ttttacagaa aatcttcttg taaaaatcga tagcagttca aaagaggagc gagtaaataa
     7921 tgatgaattt atccaatcga tttagtccta ggctttctag aatagaagta tcaaaaatac
     7981 gacaattcga tcagcaaata tcgtcgatac cagatgtgat caaactcacc ctgggagaac
     8041 cggattttcc aactcctgaa catgtcaagc aagcaggaat agcagcgatt gaagaagact
     8101 tttctcacta taccggaatg agaggattag aggaactgcg ggaagcggct tgtatatttc
     8161 agcaacagcg ttatggatta acttatgatc cgcaaacgga agttttgacg acagttggtg
     8221 caacggaagc gattgcctct gcgttgttat ctgttttgga agaaggagac aaggttctta
     8281 ttccagcgcc tgcttattca ggatatcagc cattagtgga actagctggt gctgaattga
     8341 tcccgataga tacttctgac acagggtttg tctgtcagcc ggaacaattt gaacgagcat
     8401 ttgaacaata tggatcagct gtcaaagcag tcattttaaa ctatcctaac aatcctactg
     8461 ggacgacatt gtctgcaaaa caattgaaag caatcgctga ggttttaaaa aaatatccgg
     8521 tatttgttat aagtgatgaa gtatatgctg aattgactta tagtggaacg catatgtcca
     8581 tagctactta tcttccagaa caaacaatcg tgataagcgg gttatccaag tcacatgcga
     8641 tgactggttg gcgagtgggc tttatttttg ctcaaaaagc ccttattgac gaactaatta
     8701 aagtccatca atatctggtg actgccgcta caacgatgtc gcaaaaagct gcggctgaag
     8761 cgctaataaa tggggtgaaa gatagcgaga atatgaaaga gcaatatcaa acacgaagag
     8821 actatctggt ccagcaattg gcgcctttag gatttgaagt gacccagcca aacggtgcat
     8881 tttacttgtt ttgcaaactc ccagctgcca ttcaaactga ctcttggcag ttttgtgtag
     8941 aattagcgga acaggcaaaa gttgcgtgta tcccaggaat tgcatttggc ccagaaggag
     9001 aaggttatat ccggatttct tatgctagca gcatggaaaa attacatgag gcctgcaaac
     9061 ggatcaaaga ctttttaaca gaacagtgat tcatagattt tatcaaaaaa acaatcgtta
     9121 attttgcggt atattctgca aggagacgat tgttttttaa atttagttca aatgaacgcg
     9181 tttcttttga ttcataacta atgtaatgtt aagtttaaat tgtttagaat gattttaaaa
     9241 gaatattcaa ggaggtttca tcaatggaaa aaatcaaagt tgtcattgta ggtgcatctc
     9301 atggagggca tcaatcgatc ttggaactat tgacacgata cggtgaaaat gtagatatca
     9361 cgctatttga agcaggagac tatatttcct tcatgtcatg tggtatggag ctgtatttag
     9421 aagacaaggt aacagatgta aatgatgtac gtaatttcag accagaaaac tttccgcaat
     9481 ccaatgttca tattttaaat aatcacgaag taaaaacgat cgatgccgat aaaaaaacag
     9541 taacggttac tagcgcatca gataacgaaa caaaagaata tccttatgac aaattgattt
     9601 tgagttctgg tgtcaaacca aacaatctgc cagtaccagg agcagatcta gaaaatgtct
     9661 acttgatgcg tggatacaat tgggcaacca aaatcaaaga aaaattaaat gatccggcta
     9721 ttaaaaaaat cgctgtcgta ggtgcgggtt acattgggat cgaagcagca gaagcaagcc
     9781 gaaaagctgg gaaagaagtc gtattgcttg atgtgatcga tagaccttta ggcacttatt
     9841 tagatgctga gatgacagat attttagaac aacacctaaa tgaaaacggt attgaagttt
     9901 tgacaaatgc aaaaatcgaa gcttttactg gtaacggaaa agttgaggca atcaaaacaa
     9961 gtgaaaaaga aatcccagca gatctagtca ttcaagctgc cggtgtgaag gcaaatacag
    10021 aatggttaaa agggatcgtc gacttagatg aacgtggctg gatccaaaca aatgaatact
    10081 tgcaaacgaa tcttccagat gtgtatgcag taggagatgc tacacttgct tattctatcc
    10141 cagcacgcaa aaaaatgccg atcgctttag cgactgttgc acgtagagaa gcacgttatg
    10201 tagtaaaaca tctttttgaa gaagtaccaa acaaaccgtt cggcggagtc gtaggttcct
    10261 ctgcattaag tgtctttgac tatcattttg cagcaagcgg attgaatagc tttacagcag
    10321 caaaagcagg agtagatgta cgagtgtctt attatgaaga tacgttacga ccaaaatatg
    10381 taccagaaga acaaggaaat ccaaaagtgt ccatccaatt attctcggat ccaatgaccc
    10441 atcaattgct aggaggagca gtattatcta cctatgatgt tacaggtcaa ggaaatgtat
    10501 tggcactagc gatccaaaac aaactgactt tagaagaact agcagaagct gacttcttct
    10561 tccagccagg ctttgaccga caatggagca tgttgaatct agccgctcaa caagcgttag
    10621 gagaagaacc atttgtagaa taagaaaacg aaatatttga aaagaggttg tgaaaaagct
    10681 gccttgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg
    10741 ctggacttat taccgcagat gcaagctttt gaacaccgtt tattttcaaa aaagggactg
    10801 tgacacgatt tgtgtaccga cccccaaaag ttagaccaaa aaatctaact tttggaggtc
    10861 ggtatttttt atggttaaat atacttttga acttaaatta aagatcgttc atgattattt
    10921 ggatggcaaa ggcgattgga ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt
    10981 aaaattcggg agaagcacaa ggattatggc tgtttacgga tgaccaatga actaagaaac
    11041 cgcggtttct ctatcaataa aaagaaagtt cagcgcttga tcaagaagct aggaataaaa
    11101 gtaacggcat acacgcgtaa atcacgtcgc tacaattctt atcgaggaaa agt
//
LOCUS       DAYYGY010000094        11140 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000094 DAYYGY010000000
VERSION     DAYYGY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.095"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..831
                     /gene="yycF"
                     /locus_tag="ACHA52_002482"
     CDS             127..831
                     /gene="yycF"
                     /locus_tag="ACHA52_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8941145.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            836..2671
                     /gene="walK"
                     /locus_tag="ACHA52_002483"
     CDS             836..2671
                     /gene="walK"
                     /locus_tag="ACHA52_002483"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8941146.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            2668..3984
                     /gene="yycH"
                     /locus_tag="ACHA52_002484"
     CDS             2668..3984
                     /gene="yycH"
                     /locus_tag="ACHA52_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8941147.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            3985..4854
                     /locus_tag="ACHA52_002485"
     CDS             3985..4854
                     /locus_tag="ACHA52_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8941148.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            4909..5718
                     /locus_tag="ACHA52_002486"
     CDS             4909..5718
                     /locus_tag="ACHA52_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8941149.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(5821..6450)
                     /locus_tag="ACHA52_002487"
     CDS             complement(5821..6450)
                     /locus_tag="ACHA52_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8941150.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            6620..7914
                     /locus_tag="ACHA52_002488"
                     /pseudo
     CDS             6620..7914
                     /locus_tag="ACHA52_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(7990..8652)
                     /locus_tag="ACHA52_002489"
     CDS             complement(7990..8652)
                     /locus_tag="ACHA52_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8941151.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            8877..9161
                     /gene="groES"
                     /locus_tag="ACHA52_002490"
     CDS             8877..9161
                     /gene="groES"
                     /locus_tag="ACHA52_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8941152.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9212..10837
                     /gene="groL"
                     /locus_tag="ACHA52_002491"
     CDS             9212..10837
                     /gene="groL"
                     /locus_tag="ACHA52_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8941153.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         3667 a         1916 c         2420 g         3137 t
ORIGIN      
        1 aaaataataa agtattttgc actttcattt taattttctc ttttgtttta taggcaaaga
       61 tgaaacaaaa gcagaaatac ggtataatga gaaggtatga caataatata taagaggtgg
      121 ctctttatga aaaaagtttt agttgtcgat gatgagaagc cgatttcgga tatcgtcaaa
      181 ttcaatttag ccaaagaagg ctatgatgtt tatactgcat atgatgggga agaagccttg
      241 gaaaaagttg ctgaggtaga acctgatttg attttacttg atttgatgct acctaaaatg
      301 gacggcttag aagttgctcg tgaggtacgc aaaacttatg atatgccgat cattatggta
      361 acagccaaag attctgaaat tgataaagtc ctaggcttag agttaggcgc cgatgattac
      421 gtgaccaagc cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa tcttcgtcgc
      481 ggtgccacgg ctgcgaagga accagaagag gcagcacctg cggaattgac gattggtgat
      541 ttgacgatcc atccagaagc ttacatggtg acgaaacgag gagaaacaat cgagttaaca
      601 catcgtgaat tcgaactact cttttacttg gcgaagcacc ttggacaagt gatgacaaga
      661 gaacatttat tacaaacggt ttggggttat gattattttg gagatgtacg aacagtggat
      721 gtaactgtac gtcgtttgcg tgaaaagatc gaagataatc caagccatcc aaattattta
      781 gtgacacgtc gtggtgtcgg ttactatttg cgtaacccag aacaggagta attggatgaa
      841 aagaaaagtc cgcttttttc gatcagtcaa ttttaagatc gctattacct ttattctgat
      901 tttattgatc tcgatcgaaa ttattggtgc ctactttata cgtggtttag aacgttccac
      961 gatcaataca tttattaaag atatgaatca aacggtggaa tcattagcaa caacaatcag
     1021 cccggaattg aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga atatcaaaag
     1081 atttattgaa aacagtgcga ctagcgatat catcgaaata cgggtcgttg acgaaaaagg
     1141 gatcatccga ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga aaaatgatta
     1201 tgttgatatc aatgatttca cgactaaacg ttatgttgct ttagataacg acgataaacg
     1261 ggtgaatatc aatgttcaac cgatcctgtc cccgaccggc gatgccgtta tcggggctct
     1321 ttacgtcaaa agtaatatcg aacaaaaata ttcggaaatc aataacacag cagtcatttt
     1381 ctttactgcc tcactaattg cggcatttat ttcaatgatc gtatctgttt tagttgctcg
     1441 ttcgattaca cagccgatcg gtgaaatgag agaacaagcc attcgaatcg ctagaggaga
     1501 ctacagtcgg aaagtcaaag tgcatggaca agatgaactt ggacagctgg ctgatacctt
     1561 caatcagtta gctgaacgta ttgaagaagc tcaagataca atggaagctg aacgcaatcg
     1621 tttggatagc gtgctatccc atatgacaga cggcgtcatt gcgacagatc gcaggggtaa
     1681 agtcattacg atcaatgata tggctgtttc cttgctagat atcaaaaacg aggaagcaat
     1741 cggacaatcg attttgacac ttttagatat cgaagaagaa tatacgttgc ggaaattatt
     1801 agaagatcca aacgaaatgg tacttgaccg ttcaaccagt acattggaaa gtgatcagat
     1861 gatcttgcga gtagactttt ctatgatccg tcgcgaatca ggttttatca gcggattagt
     1921 agctgtcttg catgatgtga cagaacaaga aaaaacagaa cgggaacgtc gtgaattcgt
     1981 ctctaatgtt tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt atatcgaagc
     2041 attgagcgaa ggtgcgtgga aagacgagga agtagcacca aattttttga aggtaacgct
     2101 agatgaaaca gaccgaatga ttcgaatgat caatgacctc ttaaatctat ctcgcatgga
     2161 tacaggtaat acccagcttc aattagaata tgtcaatttc aacgagttag tgaattttgt
     2221 tcttgaccgg tttgatatga tggtgggaaa tcaggaaaaa aattataaga tccgtcgtga
     2281 atttacgcaa agagatcttt gggtcgaagt agatacagat aaaatcatcc aagtagtcga
     2341 taatatcatg aataacgcga tcaagtattc accagatggg ggaacgatta cttgtcgctt
     2401 attagaaacg cataataacg tggttttaag tatcaccgat caaggattgg ggattcctaa
     2461 aaaagatctg aaccgagtgt tcgagcggtt ctatcgagta gataaagcac gtgcaagagc
     2521 acaaggtggg acaggacttg gattagctat ttctagagaa gtgattaagg cgcatcgagg
     2581 cgctatttgg gcagagagca aggaaggaaa agggtcgact ttttatatct cgctgccata
     2641 tgaaccttat gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt tcgtatagga
     2701 ctcattttga tggttgcgtt aagcatatac ttctcttatg caatctggct gagcccagca
     2761 ggaaaaagtt caatcaacgt agatgagaca aacactcaaa tgatcgagtc tcaaagcaat
     2821 cgaaaaactt ccgaagtctt tcttccgctt catgttacat ggcaccattc gggaatgatc
     2881 caagaaacca acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga taaagcaaga
     2941 tttggaaagc tttctgaagt agtttcagga gacaaatcac agtttgaaca ggtgaaaaat
     3001 ctgaactctg gttttgagtt ggcctataat gcgccttttt tgctaagaga atacaaggaa
     3061 gcatttgatt tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa tatgtatttt
     3121 actagaatac aattcgatca aggaaataaa aaaatccgat ttttgaatta ttcgaaccat
     3181 ctcgtatatg aagcaaatat ttcgttgaat tggacagata tcaataaaga attaacagca
     3241 aaagacacaa aatggacaga aatgacaaca gaaccatcga cgatcgacac gcaatatgct
     3301 acgaaacatt cgatcaaact gaaaaagtat agttatatcc tgtcacagca accctataca
     3361 ctttttagaa atgctttttt ccagaaacca gatagcgtga aaaataatga tgaaagcaat
     3421 gaattgattt tttacggtgg gaatgaaaca atgtctatcc attcggaaac gcaagtagtt
     3481 gattttcgtg gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc tgaaagtttc
     3541 ccctatatta gcaaattagg cagttcttta gggaacctca gatattttga ccgcagtgac
     3601 aatacgatcg attatcggat attcgtggaa ggcttcccgg tttttgggac agacagcaga
     3661 ggacagattg gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt gaatatccaa
     3721 accagtttga atacgattca agtgccgata ccttcagacg aagaagtaga acttgcaagt
     3781 acgaatgaaa tcatccagca gttaaaagct aagggagcag atagcaacaa gattggttca
     3841 gtgatcgttg gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt tgatctctta
     3901 cctgaatggt atgtgaaata taaagataac tggtattcag ctaacgacct tctggaaaaa
     3961 ttacctggat tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat atttttcgtc
     4021 gcttttttag ggttgaatgt gtttttattc agcagttatc atgacgcggt ataccaagaa
     4081 aacaatgtca ttcgctctaa tcagactgaa tcaatccaac aacgtctggc tagcgacgat
     4141 atcacttaca cggggaagat ttcgtctgag aacaagcaag gctattatct tagcgcggag
     4201 cagaccaatc ttagcacttc attagcgaat tatcgcaagg ctggtaaaag cggcttgctg
     4261 gcaagtggtg tgtttttaga cggtgatgtt ttgacgaaat ctttatccag ttcagatgct
     4321 gaaaagaacg tgatcgatcc agataaaatt agtagttcct tggaagattt tttatctaac
     4381 aaagacaata ttttattcgg taatgattat gtgtacacga aaaacttttc tcatttagaa
     4441 gaagaaccac tagaaatcca agcaagccaa gagtatgaag gcatcccatt caatgatgat
     4501 acagcaaaaa tcgttctttc tttagaaaaa aacgataatg attatagcat cacgaaatac
     4561 atgcagacgc atttatctga tatcgagcag ctgcgcgaaa agacggaact gtatacggaa
     4621 gaggaagcaa tcaatacatt gtacatcaat aacaaaatac caagaggatc aaaaatttta
     4681 tggcgtcagc tagcttattc ctgtattctg aaagtccggg aaaaaaatgt gtatgttcct
     4741 gtttggcatg tagcgatcga aacaagcgat aaagtggtcc aaattgagag tgtcaacgct
     4801 ttcagcaata cgatcgtgac aaacaatact gtaccaaaag tggaagatca ataaaaaagc
     4861 gagtataatg aaaaaagttt acaaagtaag aagggaagta ggagtcaaat ggctgaagaa
     4921 aaaccagctt ttaaaatcag tatcctagca agtggaagtt cgggtaattc actttatatc
     4981 gaaagtgaaa aaaaacggct tttggtcgat gcaggactta gcggtaaaaa aatcacttcc
     5041 ttgatggctc aaattgatcg gacaccggac caattagatg gaatacttgt gacgcatgaa
     5101 catcgagatc atatacatgg cgtgggtgtc ttggctcgta agtatcattt ggatgttttt
     5161 gccaatcagc agacttggga agcgatggaa ccactgattg gaaatgtacc agtggaacaa
     5221 aaacatctat ttgagatggg gaaagtcaga acattcggcg atatcgatat tgagagtttc
     5281 ggcgtctccc atgacgcagc ggctccgcaa ttttatcgtt tccataaaga aaatcggtca
     5341 ttcgtcatgt tgacggacac cggatattgc agtgattata tgcgaggtgt cattgaaaat
     5401 gccgatggtt atttgatgga aagtaatcat gatcttgaga tgttacgcat gggaccttat
     5461 ccgtggaatt taaaacagcg tattttagga gatagaggac atctttccaa cgaagacggt
     5521 gcacttgtca tgactgatgt gatcggtaat cggaccaaac gaatctatct tggccatctt
     5581 agcaaggaaa acaatatgaa agaacttgct catttaacaa tggaaaatgt tttgaaagaa
     5641 aaagattttg gcgttggaca tgattttgaa atctatgata cagatccaga tgaagcaact
     5701 gagctatttt cgatttaaac agttcaatca ataaaaatat aaaaagcaga agataaaatc
     5761 aatcagggtt ggttttatct tctattttta tttttttagc atattaaaaa aataaccgta
     5821 ttatcgactg actacactga tgtgctgctt ttcatgtttc tcatccagta tctcatctac
     5881 taatgcaatc gcgtaatctg ccatgctgat cgtactgtcg ccagcagcgt ttctttgtaa
     5941 ataatcatca cttaacaaat aagtacctgt acgtttgcca tgtggatcaa ataaagaagc
     6001 tggactgata taagtccaat gtagattttt agattgtttc aattttaaca atccttcgta
     6061 catattgaaa gctgttggat agaagggtgc atttttaggt acttggtcaa tcatacgagt
     6121 cgtcttttct gtatctaaga ataacgaaga ggcaccccca acgacaatta agcgtgtatc
     6181 ggttccttca agcatctcaa tcaaatggga caaagaagtt tgatgcattt cttcttttcc
     6241 tttgggagcg ttgaaagcat caacgacaac atcaaaagat gcaagatcag tagttgtcaa
     6301 ctcaaataaa tccttttgta taaacggaac atctgatggc aatttttctg gatgacgcac
     6361 gattgccgtc acgtctaaat ctctggacaa tgcttcgtct aaaataaatg aacctgcatg
     6421 gccagtagct ccaatgagtg caattttcat aaaaaacctc cttttttatt cctgtccatt
     6481 gtaagcagtt gctgaatatt cgtcaaagaa tccgattttt gaaaagctca gtgcgcattc
     6541 ctttcatggt tcgtgatcat tgggtatagt taaattaaga aaactgattg ataaaaatag
     6601 aagttgaagg agataagaaa tgaaagagtg gttagatcgt gcctttttac caatgataac
     6661 atctgttgtg aatcttagac ccttaactgc tttgcgtaat ggaatgactt acacgatccc
     6721 aataattatt gttggctcgc tgtttttctt atttgctcat tttcctgtgg attacatcgc
     6781 tcagtggata gacaaaacag gattttctat gtattttaca caagtatacc aggctacatt
     6841 tgatgtgata gccttttgga tcgtttttgc cattgcatac gcttatgcaa aagaagaaga
     6901 catcgatcgc ttaccagtcg gcataacagc cttgtctgct tatctactgc ttattcagcc
     6961 actaaatact cagatagata ctgtctggac aggtgctgaa gggatactag gagcagttac
     7021 aactgggctc atcatcggat ggggctatat gcacgtttag taagacttgc gaaagggtgg
     7081 aagcaaagag agaagatacc tgaaaatgtt tggcagtctt ttacttcatt gattccaatc
     7141 attgttatag tcacttttac gcttatcggt tcagcggttt ttcaacagtt gttcagaatg
     7201 actgttgttc aaggagtatg gacatatgtc cagcttccca ttcaatcgtt catagacacg
     7261 ttgtttggtg tgcttttatt aggttttttt gttccgtttt tatggttatt tggtcttcat
     7321 ggatctgcga tggtcaatgg gttggttagt cccgtcttgc aagcgaattc attagcgaat
     7381 gcagaaatct tagcctcagg acaagagttg acggtggcaa atggcggaca tatcgttact
     7441 cagcaatttc tcgatcagtt catgacggtt acgggtgctg gattgacgtt aggggctgta
     7501 ttttttatga cggtttttgc gaaatcgaag aaatacagag aattaggaaa tcttgctctt
     7561 ttaccagcct ttttcaacat caatgaatca attatttttt ccacaccgat cgtgatgaac
     7621 ccgatgatgg ctgttccttt tattttcgca ccggtcgttt caggattgat tacctattcc
     7681 gctttatatt ttggattcgt tcctttattt acagcagtcc aagtcccttg gacaacaccg
     7741 ccgatcctct cgggtttctt agtaggcgga gtgcctgcag cgattttaca aggtatcgtg
     7801 ttgtcaatta gttttttcat ttatttccca tttttgaaaa aaatagattc tgcaaactgc
     7861 aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa agatgaacga ctaaaaaaca
     7921 aaaatactcc tgtcgcttta gattgggaaa atcaccaatc taggatgaca ggagtattta
     7981 tagtcatgtt tatactagcc ctttttggac agctaattga acaagaacaa ctaatgtgtt
     8041 cattcctacg tgtgtgatca tcgatgtcca gatccgacca gttgctttgt actctaggct
     8101 gaagaagaaa cctaggaaaa agtagattag cagatgacca tcattatgag caaacgcaaa
     8161 cagcaaagag ctgatcactg ctccgaccca aaaatttgaa tatcgactga tgatccccag
     8221 tatactgcgg cggaatacga actcttccat gatgggtcca gcaacagttg tcgccaaaat
     8281 aaaagcgggt gcttccatga tcatctggat gatattttgt gtattctcgg aaggggttgc
     8341 tttgccaaac aatacagatt ctaacatgac agcgatgctt tgcagaatca atgcgacaaa
     8401 gattccaacc aatccataaa taacaatctt tggagcagaa gcttttttta ccgctgattc
     8461 gatcgctaag ggttcacgtt gcttaaaata aaaaatagct aggacaacgg cgcctagtat
     8521 atacatgact gcagttgctg tgatcaattc agacgtagat tttacaagtc ctatcgaagc
     8581 taggatcaag ggtgagataa aaattagacc atagcaaaaa atgctgagga agctatattt
     8641 ttttaaagac atgtaattcc tccttttaag ggataacaag catgcaaggc aagcttatat
     8701 cgattgattt caattatatc ataatttcaa taagcagctg tcagaataaa atttcttttc
     8761 tttgatttaa aagcttgcaa aaaagaggga gaatgagtat tattaaaaat gtaggttagc
     8821 actcgaaggt taagagtgct aacagattaa aaaaagagtt atcggaggga tttatcgtgt
     8881 taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa agaagaagaa aaaactgtcg
     8941 gaggaatcgt tttagcttct gctgctaaag agaaacccca aactgggaca gttgttgcag
     9001 tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc tgccgctgta aaagcaggcg
     9061 accaagttat gttcgaaaaa tacgccggta cagaagtaaa atacgaagga aaagaatatc
     9121 tgatcgtcgc aggaaaagat atcatggcga ttgttgagta aatttggaag ataaaaaaat
     9181 aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa gaattgaaat ttgcagaaga
     9241 cgcacgcgca gctatgttac gcggggtaga taaattagca gatacagtga aagtaacatt
     9301 aggacctaaa ggccgaaatg tcgtactaga aaaatcttac ggctctccat tgatcacaaa
     9361 tgatggtgtg acgatcgcaa aagaaatcga attagaagat cattttgaaa acatgggtgc
     9421 aaaacttgta tcagaagtag catcaaaaac aaatgatatt gcaggagacg gtacaacgac
     9481 agcaactgtt ctgacacaag caatcgttcg cgaaggttta aaaaacgtaa ctgcaggtgc
     9541 taatcctcta ggcattcgtc gcgggatcga attggcaaca aaagcagcag tcgaagaatt
     9601 gcataatatc tcaactgttg ttgattcaaa agaagccatt gcacaagtag cagctgtttc
     9661 ttcgggttct gataaagttg gacatttgat tgctgatgca atggaaaaag ttggaaacga
     9721 cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca gaactagatg ttgtagaagg
     9781 tatgcagttt gatcgtggtt acttatcaca atatatggta acagataacg acaaaatgga
     9841 agctgttcta gaaaacccat atatcttgat cacagacaaa aaaatctcta acatccaaga
     9901 tattctgcca ttgttagaac aaatcttgca acaatcacgt ccattgttga ttattgctga
     9961 cgatgtagat ggtgaagcat tgccaactct tgtattgaac aaaatccgtg gaacattcaa
    10021 cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt aaagcaatgc ttgaagatat
    10081 cgctattttg acaggtggta cagtaattac tgacgatcta ggtttagaat tgaaagatgt
    10141 aacaatcgaa aacttaggaa atgcttctaa agttgttgta gataaagaca atacaacgat
    10201 cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt gtgcaattga tcaaaaacca
    10261 aattgctgaa acaacttctg attttgaccg cgaaaaatta caagaacgct tagctaaatt
    10321 agcaggtggt gtagctgttg ttaaagttgg tgcagctact gaaacagaat tgaaagaatt
    10381 aaaattacgc attgaagatg cattgaatgc tacacgtgcc gcagtagaag aaggaatggt
    10441 ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa gtttctgctg tagaagcaga
    10501 aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct ttagaagaac caattcgtca
    10561 aatcgctgaa aacgctggtt atgaaggatc agtgattgtt gataaattga agaacgtgga
    10621 actaggcact ggtttcaatg ctgctactgg agaatgggta aacatggttg aagcaggtat
    10681 cgtagaccca actaaagtaa cacgttctgc cttgcaaaat gccgcttccg tttctgcctt
    10741 gttattaacg acagaagcag tcgttgcaga taaaccagaa ccagcagcgc cagcagctcc
    10801 tgcaatggat ccatcaatga tgggcggtat gatgtaagaa aaagcaactt gcttatctac
    10861 aactgatcat tagtgaaaga cagataaaaa gaggctggaa cagaagcgtt taactccaag
    10921 aaataagaag aaattcacgg aaattgcttt tcaaattttt gtgaatttca gcttatttcc
    10981 gaaggagttg cttctgcttc cgctgtttat acgtgtttag agcgtgagac aaaagtgttc
    11041 tttacttttg tcccacgctc ttttttgtgt gtaattataa tgaaaatagt tatttttaat
    11101 agttataata ttttatctat taaaagtaaa tatccattca
//
LOCUS       DAYYGY010000095        10636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000095 DAYYGY010000000
VERSION     DAYYGY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.096"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACHA52_002492"
     CDS             23..646
                     /locus_tag="ACHA52_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941154.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACHA52_002493"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACHA52_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8941155.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACHA52_002494"
     CDS             2142..3023
                     /locus_tag="ACHA52_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8941156.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACHA52_002495"
     CDS             3026..3796
                     /locus_tag="ACHA52_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8941157.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACHA52_002496"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACHA52_002496"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8941158.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACHA52_002497"
     CDS             5107..6270
                     /locus_tag="ACHA52_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8941159.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACHA52_002498"
     CDS             6387..6797
                     /locus_tag="ACHA52_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8941160.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACHA52_002499"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACHA52_002499"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8941161.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACHA52_002500"
     CDS             9370..9654
                     /locus_tag="ACHA52_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941162.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACHA52_002501"
     CDS             9814..10050
                     /locus_tag="ACHA52_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8941163.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACHA52_002502"
     CDS             10047..10445
                     /locus_tag="ACHA52_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8941164.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3713 a         1773 c         2252 g         2898 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggct
//
LOCUS       DAYYGY010000096         9780 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000096 DAYYGY010000000
VERSION     DAYYGY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.098"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..3366
                     /locus_tag="ACHA52_002503"
     CDS             88..3366
                     /locus_tag="ACHA52_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8941165.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3907..5814)
                     /locus_tag="ACHA52_002504"
     CDS             complement(3907..5814)
                     /locus_tag="ACHA52_002504"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8941166.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5944..6819
                     /locus_tag="ACHA52_002505"
     CDS             5944..6819
                     /locus_tag="ACHA52_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8941167.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6899..7873
                     /locus_tag="ACHA52_002506"
     CDS             6899..7873
                     /locus_tag="ACHA52_002506"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8941168.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8053..9198
                     /locus_tag="ACHA52_002507"
     CDS             8053..9198
                     /locus_tag="ACHA52_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8941169.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9335..9679
                     /locus_tag="ACHA52_002508"
     CDS             9335..9679
                     /locus_tag="ACHA52_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8941170.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3186 a         1801 c         2063 g         2730 t
ORIGIN      
        1 ttataatttt gttttttatt gacagtaaaa ataaattagg taatacttta agtaaacgct
       61 ttctaatttg ttacgggagg aatacacttg ttaaaaaaac gtataaaata cagtgcgcta
      121 atgatgaatc tccttatgct cagtaccccg gttttaattt ctgtcgttga agttgctgct
      181 aacgaacaac agtctgtgaa cgaagaaaat gcacacgtga acattgaagg gatgtcaaca
      241 atggaggaaa taccaagaac tagtgaagat atggatacgg aagaaggaaa tgatcctatg
      301 atgaaagaat cagaaaatga aaagacaaaa aagcaatctg aagaagcaaa tgaagagtgg
      361 aaaggggttg ttttcggaga aagcacaagt gctagtaggt atgccattga accattagaa
      421 gacggcgtgc ggctctcttc cacgaataac ggcggaaaat tccagtcaag cggatcagat
      481 ggaatgacct attattatca agcaatcccc aaagacatca actttaggat gcgggcgacg
      541 atcgaaatag aatcttggac ctatacgaat gcccaagaag gatttgcttt aatggtaaga
      601 gacgcagtac caaaaaatca tttgtttggg caggcatttt attcaaattc atttgccata
      661 ctaggcagtc ggatcgaata tgtctgggac tcggatagac aagaagtggt caacacgagc
      721 ggcagcaaat atacgatgcg acttggattg gggacacggg caatcacagg aatatcatct
      781 gaagatccgc aagctgcgcc agctcctggg agtgtctcag tggaaacaac acctttagag
      841 acttcagcag ggttcttgga aaaagaaaca ggttcttata atatattcgg aaacggtcat
      901 gggaatctgt ttccggccac gaactcgatt actaaactgg acgtagagat gaaaaaaacg
      961 aatactggtt tagaggcata ttattacgat cctgaaacag gagaagaaat ggcttctgac
     1021 atcatgtatg actgggaaaa attatacgct tcagatgaat cggttattta tgcaggattt
     1081 gctgcagcaa ggaatatgac gatcaaggta gaaggaatcg aagttgctta ctcagatcct
     1141 gcgacagatc ctcctgggca agaaagacct actcgcttga ttgatccgat ttacactgtg
     1201 acctctagta atcattcagg aaaccaagat catactatgt cctttagagc aaatgctgac
     1261 ggattattga caattaaaaa taaagcaaca ggagaggtgc ttaagaacgc aaaaaacttg
     1321 gctatcactg cgaatcggga atttgattat cagacaaaac tgatggaagg aacgaacgaa
     1381 tttactttca gttttacgcc agataaaaac tatccgccag aagaatacgt agagatgact
     1441 agttatgaaa caaaagagat tcttcataca gtggactacc gtaaacctaa atattcgaca
     1501 gtttatgtaa caccagaagg gtcagatgca ggaaacggaa gcagacaaaa tccattatct
     1561 ctgacacaag cattgagata cgcatatgct gggcagacaa tcgtcatggc gcctggaact
     1621 tattccttcg aaagaggatt gacgattcca aaaggcgtta acgggacaag agaaaacccg
     1681 attcagttga ttgcagagaa aaatccagaa aacaagcgtc ctgtacttga ttttaaagga
     1741 actggaaatg ggatgacact gttcagtcac tactggggtt tacgagggtt tgacattaca
     1801 aatagtgcag ctatgcaaaa aggattacag atttctggaa atcataattt gatcgaagag
     1861 attcatgctt atcggaatgg aaatacagga atccaaatca gcggtagctc aaatgatcct
     1921 ttcgaagcat ggcccgcgca taacttagtc aagaactgta catcttttga aaatgccgat
     1981 ccaggatttg aagatgccga tggatttgcg gccaaactga caataggaga aggaaatgta
     2041 tttgacggtt gtatcgcgta tcataatgca gatgacggtt gggacctttt tgctaaaaat
     2101 gaaacaggtt cgatcggaaa agtaatcatc aaaaactcag ttgcttatcg aaacggctgg
     2161 gtaccaggaa ttgagggaga aggtaacggt aatggattca aaatgggcgg ttcttcttta
     2221 acgggccctc atgaattgat caatagtttg tctttcgaaa atcttgcgaa aggaatcgat
     2281 tccaatagcg gaacagatat tatcgtcaat agcagtacaa gttttaataa cggttcatat
     2341 aatgtggctc tttatacctc ttcagcagga aacacagact accatgcttc tggtatctta
     2401 tctttccgaa cagagaatct tgagatgaga gagcagatcc gcccattgaa tcaaaacgaa
     2461 gatcaaatct accactcgtt gaactactat tggaatgaac gagagaaaca atcagaaaat
     2521 actcttggac ataccatatc aaaagattgg atcgagtctc taaatacatg ttcgaaagaa
     2581 ggtcagcagc ctgttacgcg ctatgaaaat ggaagtatcc atgttcatgg actgatgcag
     2641 attaaaccgg ggaatcgtca aacagaaaag gaaagagtgg atggtcttcc attatctgta
     2701 ggtgctattt ttgacggaga aacctcaccg tcttcggaat atccagagta tgcaatcgta
     2761 caagaaccaa atcagacaaa accaaatgaa caaaataatc cgattattat acaagaagca
     2821 aatggtgtgg cggagaatgc agaaggaaat atcattgaac tgaagccttt tacagttttt
     2881 gtgtcaactt tcacaccaaa tcaaggaacg cctgtctttg aagatatgcg ttatttccca
     2941 gaaaagatcc aaatcgttga aacacagtca agtgctcaat tttctaatca taccgctatt
     3001 ttttcagcac cttcaaaagg agaatatacg ctagaagtca cttatcgttc ggaaatattt
     3061 gatggggaaa aatggtctgc tcatacagaa tctgttaccc agcaaaaagc gatcactgtg
     3121 cgagaaactg ccgatgtgcc cccgggaaat gacaaaccag aaagagaaaa tccaggaaat
     3181 gaatcaccaa atggaaaacc aaatggaaaa ccttctcatc cggataggac agcaaaagaa
     3241 agtgaaaaaa atggcactca aggactccca aaaacgggag aatttaataa cccgttgcta
     3301 gcattagctg gaggaatttt gttaatcggt gtagttgttt atgtcatgaa acagcgaaaa
     3361 aaataagtgg aatttctcct tttatgatgt aaaacaattg actgaggaaa gtaaaaatag
     3421 aaggaaatga gaccaataga tgtctcactt ccttctatct ttttatagat gaggttctcc
     3481 ttgctgcttt tgattggctg ttcttattac ttgatgcagg acagcttttt cacaatctct
     3541 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg gcaaaatatg
     3601 aggaactatt tttccgtttt tttctctaat actcaggtca ggacgctgat ttcacaacct
     3661 ctcgataaac ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg
     3721 tggaaagcca ttttcacaaa atttttctta ctacttgaaa ctaagcagct ttttcacaac
     3781 ctctaataca tggtattcca gaagtaactt cttcgaaaaa taagccgaaa tgtcacaaaa
     3841 atttgagaag cgattttccc aaattccttc ttatttcttg aagctgacca cttctggcac
     3901 aacctcttac cagattcgca ctcttttttc tggcgctaga tacatagcgt cttgttccgt
     3961 gataccaaat gtttcataga attcgtcaag gttttttggt tgcacgtttg ctcggagttt
     4021 agcaggggca tggacatcga tttgcagtaa aagttgttgg tattcttttt ttgcttttgt
     4081 acgccaaatc gttgcccaat tgacaaagaa ttcctcgata gaaagatctg attccgtttt
     4141 tgctgcttcc aatgcacagc ttagaccgcc tgcatcagca atattttccg aaacggttaa
     4201 ttttccgttt acttttccgt cagcaaaagg cagaccatcg aattcttcga tcattgcttg
     4261 tgctttgttt tgaaattgtg taagatcttc ttctgtccac cagttgttca agttgccata
     4321 ttcgtcaaat aaagcgccgt tattatcaaa tgcatgggaa atctcatgag cgataacagc
     4381 tccgattcca ccataattag cagagcttga ttgttctaga ctgtaaaatg gtgcttgaag
     4441 gatcgctgcc ggaaagacga tcacattccg aaatggatga taataagcat tgaccgtgtc
     4501 agcactcatt tcccattctg aacgatccac tggtttgttc catttgctga aacgatcttt
     4561 taatgtgagt ttgctgaaaa caagagtatt tgaaagcaaa gtaccaccct gatctgcagg
     4621 tactgttttg aactggtcga ataatgctgg gatcttatcg gggtagccta cttggatacc
     4681 taagttattt aattttatga cggctttttc ttttgttgca tccccaagcc agtcattttc
     4741 ttctagacgt tttttataaa cggcgatcat ttttttcacc atttgttcta cgtctttttt
     4801 agcagcttcg ccaaaatatt tttttccgta gtaatcgcca actacttgat cgaattgtcc
     4861 agaggcaagg taatacgctg cttttttctg aggcattgct tcgtctgttc cagacaatac
     4921 gcgagaatag attccgctga cttggcggaa ttcttctgat agataaccac taagagaacg
     4981 tactgtttta atgatcatcc agcttttgaa taacggaaaa tgagcttctg tcaggatgtc
     5041 attcaaatga tcgaagtaca ctggatcagt tacgatcact ttatctggag ttgtcccaat
     5101 caaacccgtg acaagttttt tcaagtccag tttatctgtg tatttaataa atgtatcaaa
     5161 agattgcgga ttatacattt tgctgtaatc tgcgttttct tctgcacttt ttacatgagg
     5221 agcaatcaat ttatcaaatt gcactgcttg ttccgtgatt gcttctgcct gacttttttc
     5281 tttgccggca agctctaata actgaacagc catatcaaag aaaacgccta ttagttgtgc
     5341 accgttggga tggtctgatg cataatacgt cttatctgga aggaataaag aaggcggata
     5401 agcaaagaag gcgttcgttt gcgcattctt catatctgca tctacatcca gattaaatgg
     5461 caatggtaat gtatccaaca gccattctgg aaattgctta ttcaattcgt cgaaagactg
     5521 caatgattca actttttcga ttataggttg aagcggttga gcatgcaact catttcttcg
     5581 gtcataatcg tttgccagtt gataatatgc aaggaaatgt ttcatttttt ctgaagacac
     5641 tttttctgga tgggcaagca gatcgtctgt ttctcccatc aaaagttggt cgatcccgtc
     5701 aactaaatct tggaaaccgc ctgtagctgg tttatctgca gggatttttg ctgttttttg
     5761 ccattcttca ttgactgctt catagaaatc ttgtttcaat cgttctttgt tcattcatcg
     5821 atcgctcctt ttttgtttcg tgtgcccatt atcttacaaa atgaaggaat taacaaatag
     5881 aacataaaaa attgaaaatt cgaacaatca aactgatttg tttttttcaa tccttcactt
     5941 ataatgaaaa aaaggaggcg atcccaactg aatcaagtag tggataaacc tttatatttc
     6001 aaagtagata aaaaaattaa aaaattagcc agcactcagc agctgcagtc aagaaaaagt
     6061 aaattgcttt ttttagctct ggtttttgaa gatcaaagtt atgtgattat cgatcaatca
     6121 ggacatccgg tagaatattc tccagcaaaa tatacttatc aagagggtct ttcttgtaaa
     6181 aaatggaagc tgatcaatga agagccaatc gagttatcta gatggatcaa cagaaaagaa
     6241 gatattcccg ttttgatcga agaaaaaagg tcaggaaagg aactcgcaaa ttgctgggtg
     6301 ggattgccag aagaacgttt tttgaggtat aagaaatggg ctaccccctc aggctatctt
     6361 tgtggtactt acgcggcagc agttctcctt gcctattatc aagactatcg taaggggtgg
     6421 atgcttccaa atgagattcg caagaaaaat acggctgatt cgttggtttt gacgaaggca
     6481 ttgcgtagcc agatccaacc actaggactt cctacgattc cttttcaggt tagtacaggg
     6541 atttctagtt ttttaaagaa gaatggcaat catgagcgtg cacgagcaac tcttctaggt
     6601 tcatggcagc gagctacaaa acgtatacgt gaaggcaaac ctgtgatgat tggtattttg
     6661 aaagtacttg gcagtacata tggcaatcat tgggtgacag cgtacgctta ttttgaaact
     6721 gaaacaggag aacgttatta caaagtacac gacaactggg gcgattatca taaagtcatt
     6781 cctgccagtt ggagtaacgg aacagtttca cttccttaag aatttgctga aattaacaga
     6841 aaatcttgga aaaatatgtt aaactatcga agaatagaga agttttgggg aggactacat
     6901 gagtaataag tatcaggatg acacgttgaa gatttttagt ttgaatggca accgtccatt
     6961 agctgaaaaa atcgcgaaag tgttcggaac agaattaggt aaaagcgtcg tgaagcaatt
     7021 tagtgatggc gagatttcca tcaatatcga agaaagtatt cgaggggatc atgtgtatat
     7081 cgttcaatca acgaatgcac ctgtcaatga ttactatatg gaactactga tcatgatcga
     7141 tgcaatgaag cgagcaagtg cgaaaacgat caatgtcgta ttgccttact atgggtatgc
     7201 taggcaagac cgcacggcga aacctcatga accgatcact gcgaaattga tcgctaactt
     7261 gatcgaagaa gcaggggcaa ctcgagtact gactttagat ctgcacacgg ttcaagtaca
     7321 aggattcttt gatattcctg tagataattt atttacgatg ccgttgtttg ctcattatta
     7381 ccgtcaatta ggtctgacgg gagatgacat cgtagttgtt tcgccaaaaa acagcggtgt
     7441 tcagcgtgca cgtagtcttt cagaatattt gaacagcaca ttagccatcg tagatcatgc
     7501 agatgatgct tcaactgagg gtggttatgt gatcggtgat gttcaaggaa agacatgtat
     7561 catggtagat gatatcttga acacaggagc aacgcttgca cgagcagcaa atgtattaaa
     7621 agaaaacggc gcaaaagaag tatatgcctg tgcgtcacat ggattattgt ccgatcatgc
     7681 gaaaagtatt ttagacaatg caccaatcaa agatatttgt atcactgatt ctgttttcac
     7741 agaagaaaat cgtcatccag aaaacttaac aatcgttaca tgttcagaat taatgggtga
     7801 agcggtcaaa cgcatccatg aaaatacacc gatgagtccg ttattccgtc tggaagaaaa
     7861 agaattcgaa taaacaaaaa aaagagggaa agaagttgtg acagaagtga gcagcttcaa
     7921 ggagctgcga caagactttt gtcacagctc cttttttcta tgcccatgaa acgtcctcta
     7981 gacaaaaatg agctcttctt tggtttgttc gctattttat gctagaatgt gcctagcaat
     8041 aatttggaag ggatgaatga agtgaaaact atttatctgg atcatgcggc aactaccccc
     8101 atgcatccac aagtaattga agcgatgacg gcaatcatgc aagacacgtt tggaaatcct
     8161 tcaagcatac atggatttgg tcgccacgcg catgaaaaat tggaaacagc aagacaaatc
     8221 attgcagaca gtctgcaagc aaaacctcac gagatcatct ttaacagtgg gggaacagaa
     8281 ggggataata cagctatctt agaaacagcg ttctcgcgtc aaaaagaagg cagacatcta
     8341 atcactacag ctatcgaaca tcctgctgtt cttaatacca tgaaatattt agagacaaag
     8401 gggtttgacg ttacatatct gcctgtagat gaaaaaggga atctgtcgat cgaagaggtc
     8461 aaaaaagcgt tacgagaaga tacgatcttg gtctctgtga tgtacgggaa caatgaaatc
     8521 ggaaatttga tgccaatcaa agaaatcggt gaacttttag cggatcatcc agcttatttc
     8581 catacagatg cggtacaagc gtacggaaac caaacgatcc atccagaaga attaggaatc
     8641 gatttattaa gtatctctgc tcataaaatc aatgggccaa aaggcgtcgg tttcttatat
     8701 aaacgagacg gagtaaatat ccctgcattg cttttaggcg gggagcagga agaaaagcgg
     8761 cgtgcaggta cagaaaattt agcagggatc tggggtatgg caaaagcagt tgaactgctg
     8821 tcacctgaag aacgtgaagc acgtaacaac aaatataaag gattccagca attgattctt
     8881 gatacattag aagcaaacga aatcccttat caagtaaatg gtgatttaac gaataaactc
     8941 cctcatgtgt tgaatcttcg cttaattggc gaagcaaatg atttgctact gatgaagctt
     9001 gatcttaatg gggtcgctat ttctactggt tcagcttgta cagcaggaaa tatcgagcct
     9061 tcacacgtat tagaagcaat gtatggagaa gatacaccga tactaaaaga atccatccgt
     9121 atcagctttg gtttgggaaa taccgaagac gacatccagt atttccttga tcaattagtt
     9181 cgtgctgtca aaaaataact agtgaagtca ttcttatatg aacagatgtc tgagagaacg
     9241 ccttcatttt ttagggaact taactgaaaa acctagtaaa aatcaggata aacttttata
     9301 ataaagagaa actaatttta tcgaggtgaa aatcatggca tttgaaacaa cagcaacggt
     9361 actgggcgca ccaattagct atcgtttgca tcctgatgca aaaagatata cattgcgcga
     9421 taatggattt acagaaacaa acggaggcaa ttatcaattg atccgaccat tagatgccac
     9481 gccgcaaagc aaagaaggat tcaaattaaa aatcactgta tcaaaagata ttcaaacatt
     9541 gaaaatgtcg atcactacag caaatggatt gaaagcagtc aatattttca aagatccaaa
     9601 acagaaaatg agccaagata aattttattt tctgatggat gggatgatta gccgtgggtt
     9661 attcgaaaaa gtcgaatgat ctgtggatat aaaaagacaa ctagttttga aacggcctat
     9721 tgttagatga ctaactttgt aaggacgacg caaattagtt gtctttttta tttttaaatt
//
LOCUS       DAYYGY010000097         9771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000097 DAYYGY010000000
VERSION     DAYYGY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.099"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..675
                     /locus_tag="ACHA52_002509"
     CDS             250..675
                     /locus_tag="ACHA52_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8941171.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(970..2103)
                     /locus_tag="ACHA52_002510"
     CDS             complement(970..2103)
                     /locus_tag="ACHA52_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8941172.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="ACHA52_002511"
     CDS             complement(2094..2762)
                     /locus_tag="ACHA52_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8941173.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="ACHA52_002512"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="ACHA52_002512"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8941174.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="ACHA52_002513"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="ACHA52_002513"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFM8941175.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="ACHA52_002514"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="ACHA52_002514"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFM8941176.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="ACHA52_002515"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="ACHA52_002515"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFM8941177.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="ACHA52_002516"
     CDS             complement(6138..6911)
                     /locus_tag="ACHA52_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8941178.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="ACHA52_002517"
     CDS             7076..7555
                     /locus_tag="ACHA52_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8941179.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="ACHA52_002518"
     CDS             7612..7914
                     /locus_tag="ACHA52_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8941180.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="ACHA52_002519"
     CDS             7936..9393
                     /locus_tag="ACHA52_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFM8941181.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9440..9667)
                     /locus_tag="ACHA52_002520"
     CDS             complement(9440..9667)
                     /locus_tag="ACHA52_002520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941182.1"
                     /translation="MRSDSGKKKSRNSQLNNKKSKSTKQTRTQKESLRNTNKGKLKGV
                     KKSINVDNFFIGCGKRWAMLFEMDFTSIYFL"
BASE COUNT         2791 a         2123 c         1713 g         3144 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc t
//
LOCUS       DAYYGY010000098         9636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000098 DAYYGY010000000
VERSION     DAYYGY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.100"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACHA52_002521"
     CDS             185..1183
                     /locus_tag="ACHA52_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8941183.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACHA52_002522"
     CDS             1295..1804
                     /locus_tag="ACHA52_002522"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8941184.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACHA52_002523"
     CDS             complement(1834..2493)
                     /locus_tag="ACHA52_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8941185.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACHA52_002524"
     CDS             complement(2495..3259)
                     /locus_tag="ACHA52_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8941186.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACHA52_002525"
     CDS             complement(3267..3944)
                     /locus_tag="ACHA52_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8941187.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACHA52_002526"
     CDS             complement(3981..5366)
                     /locus_tag="ACHA52_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8941188.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5335..5589)
                     /locus_tag="ACHA52_002527"
     CDS             complement(5335..5589)
                     /locus_tag="ACHA52_002527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941189.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACHA52_002528"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA52_002528"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHA52_002529"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHA52_002529"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8941190.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACHA52_002530"
     CDS             6305..7162
                     /locus_tag="ACHA52_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941191.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACHA52_002531"
     CDS             complement(7243..7578)
                     /locus_tag="ACHA52_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941192.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACHA52_002532"
     CDS             complement(7645..9015)
                     /locus_tag="ACHA52_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8941193.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACHA52_002533"
     CDS             complement(9093..9629)
                     /locus_tag="ACHA52_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8941194.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2823 a         1988 c         1618 g         3207 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacc
//
LOCUS       DAYYGY010000099         9283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000099 DAYYGY010000000
VERSION     DAYYGY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.101"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1512
                     /locus_tag="ACHA52_002534"
     CDS             61..1512
                     /locus_tag="ACHA52_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8941195.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            1505..2227
                     /locus_tag="ACHA52_002535"
     CDS             1505..2227
                     /locus_tag="ACHA52_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8941196.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            2247..3398
                     /locus_tag="ACHA52_002536"
     CDS             2247..3398
                     /locus_tag="ACHA52_002536"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8941197.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(3746..4021)
                     /locus_tag="ACHA52_002537"
     CDS             complement(3746..4021)
                     /locus_tag="ACHA52_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8941198.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            4129..4893
                     /locus_tag="ACHA52_002538"
     CDS             4129..4893
                     /locus_tag="ACHA52_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8941199.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5017..5520
                     /locus_tag="ACHA52_002539"
     CDS             5017..5520
                     /locus_tag="ACHA52_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8941200.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            5785..6432
                     /locus_tag="ACHA52_002540"
     CDS             5785..6432
                     /locus_tag="ACHA52_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8941201.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      6475..6542
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6593..7861
                     /locus_tag="ACHA52_002541"
     CDS             6593..7861
                     /locus_tag="ACHA52_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8941202.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(8038..9240)
                     /locus_tag="ACHA52_002542"
     CDS             complement(8038..9240)
                     /locus_tag="ACHA52_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8941203.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
BASE COUNT         2958 a         1615 c         2000 g         2710 t
ORIGIN      
        1 tgaatattta ttcttttata gcttttctga ttacattatt attcaaaggt ggccaaatgg
       61 atgaaaaaag gaataaacat attattagtg gcgctgatgt ttctactggt ttttttacca
      121 gtaaaagtac aaggtgcaga aaaagatgaa atttacgagc ggataaaaca aagtggagaa
      181 ttagtcgttg gcctatctgc agactatgcg ccctatgaat tccatgcgaa tgtggatggt
      241 aaggataaaa tcgttggttt tgatgtatcc attgccgaaa agatcgcaaa ggatatgggt
      301 gtgaaactaa aagtagaaga acttggtttt gatgcattat taggtgcgtt gaaaactggc
      361 aagatcgatt tagttatatc tggtatggcg gtgactgaag aacgtttaca agaagttgac
      421 ttttctgacc catatttcgt ggtacagcaa aaagtactcg ttcgtaaaga ggacaaaaat
      481 aaatttaaga cgacagccga cttttctggc ttagccgttg gggcgcaaaa gcaaaccaca
      541 caagaggagt tagtgaaaaa tgaactgact ggtgcagagt tgacctccct gcaaaaagtt
      601 cctgatctga tcaataattt gctgaatgaa aaagttgaag gtgttgtgct ggaacaacca
      661 gttgcagaag catatgccca acagaataag aaagtagctt tgactccagt aaaatttgcg
      721 caaggcgaaa aaaccacagc tattgcgatg tcaaaaaata caccagaact gaaaaatcat
      781 gtcaatgcgt caatcaaaac gatcaatgac gacggcttgc tgaaagatta tcaaaaacaa
      841 gcgaatcaat tgatgttcca agatgatcag tcctttcttc aaaaatacgg tccgtattat
      901 ttgattggaa caaaatacac tattttatta gcttttgtgg gtgtattgtt tggaatggtc
      961 tttggtagtt tgcttgcact gatgaaattg ggtaagaata aatttttaaa ggcattagcc
     1021 attatttata tcgaatacgt acggggaaca cctctcttag ttcaaatctt tatcgtttac
     1081 ttcggaacag gagtactggg attggatcta tcgaaactgg tagcaggttg tattgctgtc
     1141 tcgttgaata gtgctgctta tgtagccgaa attgtccgtg ccgggatcaa tgcggtcaac
     1201 aaaggacaga tggaagctgc acgttcttta ggaatggacc agaaagaagc catgcgtttc
     1261 atcatcttcc cacaagcaat caaaaatatt cttccagctc taggaaatga gtttgtcacg
     1321 atcatcaaag agtcctcagt tgtttcggtc attggggtat cggaattgat ctttcaaacg
     1381 ggcgtggtac aaggtgctag cttcaaacca ttcctaccat atgtgttggt ctctttgatt
     1441 tatttcgtgt tgaccttcag tatttcccgt atattagctt tagttgaaag gagaatgggt
     1501 ctgaatgctt aatgtaaaaa aactgtcaaa aagttttggc gagaatgaag tcttaaaatc
     1561 aattgatctt tctgtgaaag aaggagaagt agtggtgatc attggccctt caggaagtgg
     1621 gaaaagtaca attctacgtt gtatgaatct gctggaagaa ccaacttctg gtgaaatctt
     1681 ttttaaggga caaaacatta cagcccctga cagcgatatc aatcaaattc gtcaaaaaat
     1741 gggaatggta tttcaaagtt ttaatttgtt tccccatatg aatgtactcg aaaatttgac
     1801 catcacacct gtaaaaatca aaaaagaaga ccctaaaaaa gcaaaagaac atgctctatc
     1861 attattgaac caagttggtc taaaggaaaa agtggacagt tatcctacca gtttatctgg
     1921 gggacagcaa caacgggtag caattgctcg agcattagca atgaatcctg atgtgatgct
     1981 tttcgatgaa cctacatctg cacttgatcc tgaaatggtc ggtgaagtgt tggcagtcat
     2041 gcaggattta gctaaaaaag ggatgacgat ggtcgtggtc actcatgaaa tgggttttgc
     2101 taaagaagtt gccgatcgtg tgatttttat ggctgacggc atcattcaag aagagggaac
     2161 accgaaagag atttttgaaa atccgcaaaa tagtcgaaca caggatttct tgaacaaagt
     2221 gttgtaacaa agatggagga agtaagatgg acaaaagtaa gattgcacgc cggtttcagc
     2281 aaccagcaga aaatctgttg atgaatattg ctattttggc aaaggaaaaa gaggatatta
     2341 tcgatttatc gattggtgat cctgacttga tcactgatcc gacgattatt gaagcagctt
     2401 atacagatgt aaaaaatggt gctactaatt atactgcgcc tgatggcagc aaagaattct
     2461 tgtcaacggt cgtagatttt tatcaaaaga aatatggatt gagcgttgaa tgcaaccaag
     2521 ttcgagctac agtcggagca atgcacggga tgtatctagt tttacaggca ttactagatc
     2581 cagaggatga ggtcatcatc catgaacctt atttttcacc atatcgggaa caagtcattt
     2641 tatctggagg caagcctgtt tttgttccta cctatgaaga aaagggcttc cagttggatg
     2701 tggaagattt aaaagcagcg attacggatc gtacaaaggt gatcgtcatc aattcgccga
     2761 ataatcctac gggagctgtt ttttctgaaa gcacgttgaa aaaaatcgct gaagtcgcaa
     2821 tcgaaaatga actctatatc atttccgatg agatttatga agactttgcc tttaaagaga
     2881 agttcgtacc gatggcgcga tttgcgcccg ataatacgat cactgtcagt ggtgtatcga
     2941 aggggtttgc gatgactggc tggcgcttag ggtacatgat cgcaccagca tacatcaatc
     3001 aaattgctgg gttgatcaat gaaaacatca cttattccgc accgactgct tctcaaagag
     3061 gtgcaatcta cgccttaaag cactatgaac agcttgtacc gccagttgtt gaagtattca
     3121 aagaacgatt agcttatatt gagaaaagag tggcagaaat cccttactta tctttatctt
     3181 ctgtttctgg aagcatgtat gcttttatta atatcagtaa gagtgggctc gattcagtaa
     3241 cgtttgtgga agaggtttta gaaaaaagcg gcgtactttt tgtccctgga aaagcgtttg
     3301 gggaaacgat cggggacaac tatattcgac tagcagcgac tcagccattg gaaatcttag
     3361 gtgaagcctt tgatcgttta gcaaaactaa atttttagat aaaactgtat atttgaaaac
     3421 agctatgggt atccattaga acaaagaggg aaaatgatcc tttcgagagt tggcttgttg
     3481 actgggcagc gtcaggattt gacaatgaaa aagtggtagt ggaaaatcaa agtcgatttt
     3541 ccactaccat aaaatattat agactccatt ttattgaacc aaaatttttg aagtcctccc
     3601 gcttttacgt atgcctatca ctggttacta aactcgctaa taagaatgtt tttttgtcga
     3661 tctaggatgg agagaggggc aaactttttc gatctacata cagacagtcc gatttttctg
     3721 acatttccta ttcatttcta gaaggtcagc ctattacaac aaactttttt ctgtcctcca
     3781 tcatcggatt ttcctgaagg tctacatatt ggacacgtcc gctggggctt ggtgatcgtt
     3841 tgacttcttc cacgaatttt ttgattgcct catcgtcgcc cgcagcttcg atcgtgacag
     3901 agccgtcatc ttcatttttc actgttccgg tgatccctaa ttgatccgct acgatttttg
     3961 tcatatagcg aaaaccaacg ctttgtacac gtccttgcac gttcatcttg attttgttca
     4021 ttttattcac cgttctttct tttattttta aattaatgat accgactttt ttgctactga
     4081 acaagtgcaa tgatctaatc catggtataa tcagttgagg tgaaaaatgt gaaagagatc
     4141 caatcagcaa aaaatccaag aatcaaagaa ttaaaaaaat tacataagaa aaaatatcgt
     4201 gaagaaaaag gggaatatct gcttgaaggg tttcacctga tcgaggaggc tgttgcctca
     4261 aatgcgaagg tcaacgaact ttttattacg caacgcggtc tgactgagtg ggctagttgg
     4321 cttgaagaaa atcaggcgct ttctgttaca ctagtatcag aagaagtgat ggcaactgtt
     4381 tcagatctac caactccgca agggatcatg gcagtggtgg aagctgaaaa aagagagata
     4441 ccgaattttg aaggtggttg gttgttgctc gacaacgtac aagatcccgg caatgtaggg
     4501 acaatgatcc gcacagccga tgcttttggt ctatcttgtg ttgtactagg taagggaacg
     4561 gcggatgtct acagtaccaa agtattaaga agcatgcagg ggagtaacta tcatttaccg
     4621 atcatccaag aggatctaac aaaactattg cctatcctta aagagcaagg atttataacc
     4681 tatggaacag aactgaatga agaagcagtg tccctgaatg atatccagac aacagaaaat
     4741 tatgcgatcg tgatggggaa tgaaggtcaa ggcgtacgtc cagaagtgct ggcgctaacg
     4801 gataaaaatg tgtatatacc gattcaagga aatgcggagt ctttaaatgt aggagttgca
     4861 gcgggcatca tgctttatca cttctccgta tagttaagga actattaagc tttctgcatt
     4921 ttgcgaaaac ctcactagaa tattacgaac actagtggta tactaagttc aatttcaaag
     4981 ataaatggaa ttcaaaaagg tggcagatcg ataattatga cagaaaaatg gcgtgaagac
     5041 gaagaatata tgtcttatat aaatgatcta ttagaaactg atgcagtaaa gaaattagca
     5101 aattacacgc aacatgtcca ctcgacacga ttagagcatt caatcagtgt gtcttactat
     5161 agctacaaat tagcgaaaaa atggggcggt gatgcaagag cgactgctcg agcaggcctt
     5221 ttacatgatt tattttacta cgattggcgt acgacgaagt tcgatgaagg tacacatgcg
     5281 tatgttcacc caaggatcgc tgtgaaaaat gctgaaaaaa tcacggagct ttcagatcta
     5341 gagcgtgata ttattttgaa acatatgtgg ggagcaacga ttgcccctcc taaatataaa
     5401 gaaggttata tcgttacttt tgtggataaa tattgtgcag tcaaagaagc agctcaacca
     5461 atgacagctt caatgaaaca aaaatggcaa cgatatttcg gtaaagaaca atcgatttaa
     5521 ggtagttaac taagtatttg acccgagtat tttgaaaaaa atcctcgggt tttttgttta
     5581 gacattgact aattggtctt tttagtgttt actttgttga gatgaaaaag tacataacca
     5641 acataaaaat aaagggggtg atgataagat gcgatgtgta tcttatttaa aagagtttga
     5701 gggctttatc taaaggcact ttgatagaaa gatcactttt ttcttcaaag tagccgattt
     5761 tgaaagaaga aaggactcaa agtcatggaa aaaacaatca aaccatatca atattatttt
     5821 atcaagcgag aagtcgagca gctgttaagc gcctatagtt cagtgaatga tccaaagaca
     5881 gtccaaacag tccaagcatt agctgcggaa aagatccacg aggtacttgg tcatcctaat
     5941 acagctgtaa atgaatttat ggaaaaggta ctagacgtca ctctaaaaaa agaaaaagca
     6001 gaacgactat tagaggaatt gaaagtgatc gttcttccat tcatacaacc gacaaaaccg
     6061 cagatcgata aattattccg aaaagtgaaa aagttaaaac aaccagattg gaatcaactg
     6121 gatttaaggg aacatactta tattggttgg aatgatccag gtagccaaaa aaaatttatg
     6181 atctattatg aagaagaaaa actaaaaggg atcagtggaa cattatctcc tacaatcaac
     6241 aaaggaattt gttcgatttg tcaaagaact tcaaatgttt cactctttct ttctaccaca
     6301 aaaactggtt cagatggaac atacacgaaa aaaggaaatt atatttgtca tgatagtgac
     6361 caatgtaatc agcagttggt acaattagct cctttttacg aattcacaga acgagtgaaa
     6421 cgtgagcggt gatttcattg tatttttctg aaaaatatgc tatcttattt ggtgaagtga
     6481 ataggtgatg gtgttcgcct ttaaacgttg atcgtaagat aactaatgac gcctactaaa
     6541 gaaacggggc tacccctttt tttagtaggc gtttttttga ggaggaaatg agatggaaaa
     6601 tcgattacct gatccattga aggtgatcat ctatatgatc aaatggttat tgatcacagc
     6661 aattttaggt atttttatgg gaagtttatc cgcattcttt ctaactagtt tgactgatgt
     6721 gacagctatt cggctggagc atccttggtt actttttctt ttgccgataa gtgggggact
     6781 ttttgcttat ctatatacga cgtatggggg caattcttcc cggggaaaca atttagtgat
     6841 cgatcaaggg aatggaggaa atgaaaagat tccattacgg ctgatcccac tgaccttatt
     6901 tggcaccatt accacgcatc tttttggtgg ttctgttggt cgggagggta cagcggtcca
     6961 aatgggcggg gcacttgctg agaattttgc tcgttggcta aaattgaaca aaatcgagcg
     7021 tgagattctt gtcatcagtg ggatcagcgc aggttttagc tcagtatttg ggacaccctt
     7081 ggctgggaca ttgtttggat tagaagtatt ggcaatcgga aaagtgagaa ccgaagcact
     7141 gtttccaagc ttttttgcag cactttttgc gaactttgta acggaaagtt tcaatgtcaa
     7201 gcatacccat tatcaaatgg gggcgattcc tgcatgggac ccttcacttt tcttcaaatt
     7261 gttgcttgcg ggcattgcat ttggtatcat tggctggctc tttagccgtt cgatcgtctg
     7321 gttaaaagct tggtatgcaa agcggatccc ccatccagtt ttacgcaact ttattggagg
     7381 agcagtcgtg gtactcgtag tagtgatttt acagacgcaa agatatctcg gattaagctt
     7441 acctttatta caagatgcat ttaatggaag tgctcatcct tatgattttc ttgggaaact
     7501 tggctttacc gttctatcac ttggcgcggg ataccaagga ggcgaagtga cccctttgtt
     7561 tgagattggg gcaactttag gtgcgacatt agcacccatt cttcatgtat cagtaccttt
     7621 tttagcagga ttagggttta ttggggtgtt ctctggagca acgaatacac cgattgcttg
     7681 ctttgttatg gggatcgaac tttttggagg tcaagcagcc agctacttct ttatgatctg
     7741 tttgattagc tttatgtgtt caggaaatag tggaatctat tctgcgcaaa aagttgaagt
     7801 gaaaaaagga atcttgttcc taccgatcct tggaagaatc tcacgacaaa aagagaaata
     7861 aaaagaggtt gggacagaag tgtttaactc cgagaaataa gaagaaattc acgaaaattg
     7921 ctttttaaat ttttgtgaat ttcgcttatt tccgaaggag ttgcttctgc tcccaccgtt
     7981 tattcggagt tagagtctgt ggcaaaactg atttttagtt ttgtcccacg ctctttttta
     8041 ttgagcaaat ttgagaataa gagcagaaaa tactgcaaag ccaatcatta agccacagat
     8101 gataagatgg cgttttttgt gtaagacaag gatggcaata aattctctta agatagcatt
     8161 cggtaagtag tatagtgcag tttttgcacc gattccgttt gcattgagtt tcgcttgtcg
     8221 cgcatatagt ccagctctaa agatatggta attatttgaa gtaaaaatcg cgcgataagg
     8281 cccattcatt tgctgatcca ttatttcttt tgaaaacagc atattttcta gcgtcgttgt
     8341 ggaattcgtt tctactaaaa tatcacgctc gggaatccct ttttctaaag catattgttt
     8401 cattgcaatg gcttcagcta ctttttcatc ggttcctttt cctcctgaca tgagcaagac
     8461 tggcggattc aaagttgctt tactttgtgc ccaataaaat gcgatcgcct tattgatcct
     8521 tttagcaagt aggggagata cgcgttcacc atcaatcaag ccagcgccga gaacaacgat
     8581 ataatcttgg ttataccttg gacggttgaa ctgatacaaa attgaaacag tcataaaatt
     8641 aaagaaaacg atgagtagat aagcaaaaat aaacggaatg acagcaaata gagcatttgc
     8701 ccattgtggg agccaatgga ctaatgtata gtccaatatc aaaaagatag tcaaagcgac
     8761 tcctaacaag agagtcaaaa ggttggccaa agaacgggat tcttttttca tgactacaac
     8821 accattccaa aataaaaaaa tgatcaaaat gtagataccg attgccaaaa gaattaatat
     8881 tggtatcccg atcaaagcca ataacccacc gataaagata ttttgcgtct ctattagtag
     8941 ccaccctaga taaccaccaa agacaatcaa aaacgtatta aaaagtacgc cattgattaa
     9001 cctacgtttt tccttaaaat agaaaaatgc aaaaatactg aaaaatagca atggaatcaa
     9061 aaaataaaat gtatgataaa aaacttctaa aacgacgaag taagtgacca tcatcataaa
     9121 agtccacgtc ccacaataaa agaatagagc gggatcgctt tggcgcttgc gccaatagag
     9181 gatgcctgac attcctaaaa tatagataac taaaaatatc aggtctctag taagattcat
     9241 tgaatgaact cctttcgatt tgtaaatttt ttcttaaaaa aga
//
LOCUS       DAYYGY010000100         8955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000100 DAYYGY010000000
VERSION     DAYYGY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.102"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(445..942)
                     /locus_tag="ACHA52_002543"
     CDS             complement(445..942)
                     /locus_tag="ACHA52_002543"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8941204.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(982..1791)
                     /locus_tag="ACHA52_002544"
     CDS             complement(982..1791)
                     /locus_tag="ACHA52_002544"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8941205.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2125..2982
                     /locus_tag="ACHA52_002545"
     CDS             2125..2982
                     /locus_tag="ACHA52_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8941206.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2975..3877
                     /locus_tag="ACHA52_002546"
     CDS             2975..3877
                     /locus_tag="ACHA52_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8941207.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3910..4260
                     /locus_tag="ACHA52_002547"
     CDS             3910..4260
                     /locus_tag="ACHA52_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8941208.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4309..5232
                     /locus_tag="ACHA52_002548"
     CDS             4309..5232
                     /locus_tag="ACHA52_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8941209.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5310..6205
                     /locus_tag="ACHA52_002549"
                     /pseudo
     CDS             5310..6205
                     /locus_tag="ACHA52_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            6243..6806
                     /gene="amaP"
                     /locus_tag="ACHA52_002550"
     CDS             6243..6806
                     /gene="amaP"
                     /locus_tag="ACHA52_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8941210.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6820..7008
                     /locus_tag="ACHA52_002551"
     CDS             6820..7008
                     /locus_tag="ACHA52_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8941211.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7021..7923
                     /locus_tag="ACHA52_002552"
     CDS             7021..7923
                     /locus_tag="ACHA52_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8941212.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7955..8197
                     /locus_tag="ACHA52_002553"
     CDS             7955..8197
                     /locus_tag="ACHA52_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8941213.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8453..8824)
                     /locus_tag="ACHA52_002554"
     CDS             complement(8453..8824)
                     /locus_tag="ACHA52_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941214.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3118 a         1548 c         1739 g         2550 t
ORIGIN      
        1 gttggttaga gcagacggct cataaccgtc cggtcgtagg ttcgagtcct acaaggtaca
       61 taagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaacagc
      121 ttcttatgtt tttacttggc tgttcttatt actgcagatg caagcttttg aataccgttt
      181 aaggaagagg ttgtgaaatc agcgttttga cctgagtatt agcttaacaa acggaaaaat
      241 agttcctcat attttgccgt ttgttaagct aataccgagg gtcagctgat tgaacaccat
      301 ttaagaaaga ggtcgtgagc ctgccgtttc actctgtgtc ataaaaaagc caatctactt
      361 tgatagaaaa agcacgtagg actaaaacag ttctgcgtgc tttttttatt gtgtgttaaa
      421 tatcgtaaat gaagatagat atttttatga ttcgacgata gagacgttga aatctttcaa
      481 cagctctaaa gcggattctc cgtgttttct actggagact aacattggat caacaaaaaa
      541 gtctgcttca ggaaaagcac tctgcaggcc tatcagatta gagagtacac acaagtttgt
      601 ttcaatcccg actacttcga tctctttttg ttcttctttg ctttcaaata aagtttcctg
      661 gatttcagct aaagtttcag gtggaatcgt aaagtaatac tttttaatca ctcgttcatt
      721 ttcttgtgga gaaagaagag ggatcaattg caaatcttct cgttcttctt ctttgtcctt
      781 cagttcaact ggtatatccc gtgtgaagag aatatattca ttattttggc gtgctttagc
      841 taatctttga gcaatacgtt ctgccaattg atctgactct ggaatataaa attcgctgtt
      901 tggatcaaga atatgatttt gcatatcaat aataactaac attttctttc ctcctacatt
      961 catatcgtca ttttattctt tttaatagaa gttttttaaa atataagcaa caccagcttc
     1021 ttcattcgtc agtgttactt ggtttgcaat ttcttttact tgtcgctgtg cattccccat
     1081 cgcaatactc aacccgctgt aattcaacat agcaatgtcg ttttcttgat cgccaaaagc
     1141 catgatgtca ctcgattcga ttccaaggta atccgccaga aaaacaagtg aatgagcttt
     1201 agtgatacct gtaggcgtga tttctaagaa atgcggatca gagaatactg attggatacc
     1261 atctgcttgc agatctcttt gtagcttctg tagtttttct tcactcttat ctgttaatgc
     1321 taatttaaga tagctaagag aaggatcttc caacatattt aaaatttgat caaaagaatg
     1381 gttctgtaaa ttgaaatgtt cataataagg gtcatctttg actagatttg attcatcgaa
     1441 tgtcagctga tcatcttggg tttctcggct agcaatgatg gatattttcg cttcttgagc
     1501 atagaagaag gcttttctca gtgtatcgct atctattttt gaacgtctga gaactttcgc
     1561 catttttcca tccttcattt cagcaatcag tgaaccattg gaagaaatca aatagccacc
     1621 gattcctaat tcggaaaaaa tatctttagc agaaatgctg ttccttccgg tagcgacggt
     1681 tacgattccg ccattttcat caaatttccg aagtgcttgg atattttctt ctggtatctt
     1741 tccatttcga tcaagcaagg taccgtctaa atcaatcgct gctagtttca ttttatcttc
     1801 ctactttctt tttacatatc tattttcctg ttaccttaag attaaatgag aatttaaaaa
     1861 aatgaaacta acttgctttg tcattatgaa ttttttcaaa aaacattctt aagaaaaagg
     1921 tggctatata tagtagtttt tggtcaatac ctttgaataa aaagccatga aaaacgaata
     1981 gttagcgctt accgatgcgt tttgtgataa aaaaatttta aaatattcaa tgaaaacaaa
     2041 gcatatattt tgctttaaaa ccaaatgagg aatattctag atgtagaaca ggttagaaaa
     2101 agattagaaa aaggagtgat tttgatggag aagagatacg gcgtagttcc tgtggtaact
     2161 gcttcagacg aaaattatgc tccttacttg agcgtgatga tcgcaactgc attagaaaat
     2221 tgcaataaaa caagacgcat aaaattttat gtgattgacg atggtctatc agaatatagt
     2281 aaagaaggat tggaagaaac ggtaaataaa tattcaagta atgcaagtat ccaattcctg
     2341 acagtggaaa aagatatcta cgaagacttc ctagtcagtg accatatcac aactaccgct
     2401 tacttaagaa tttctttacc aaacttatta gctaaagagg attataagaa agtcctttac
     2461 ttagattcag atgtgttagt actagacgat atcgtaaaac tatatgatga gccgttaaat
     2521 ggaaaaacaa tcggcgctat tatcgatcca ggccaagtga aagctttgga aagattagga
     2581 atcgattccg atgacttgta cttcaattca ggagtgatgg tgattgacat tgatcaatgg
     2641 aataaaaaag aaattacaga aaaaactatc cattacctca gtgaaaatgg cgatcgaatc
     2701 atctatcacg accaagatgc tttgaatgct gtactttatg aagattggga acaacttcat
     2761 ccaaaatgga acatgcagac ttcgctgatt tttgaacgtc atccagcacc aaatgaaaaa
     2821 tatgaacgtc agtataaaga aggaaatgaa aaaccttcga tcgttcactt tacaggacat
     2881 gataaacctt ggaatacgtt gaaagatcat ccgtatacca atttatactt aaaaaaatta
     2941 gcacacagta ctctaacgaa agtaggcgag atcaatgaat aaaaaagaaa tagcagtagt
     3001 cgcaagttgt aacacaaaat tcgtaccaca tttagctgca ttatttgtct cagtattaga
     3061 taattgcaac ccgtctaaat ttgttcggtt ctatgtgatt gacgatgata tcgattttga
     3121 aagtaaacag cttttacgct tctcagtgaa aaacgcacga atgaactcag atgtggaatt
     3181 cttgaaaatc aataaagaat tctttacaaa tgtggtaatc agcgatcgta ttccagaaac
     3241 agcttattat cgtattgcga ttccggaatt gttccgcggc acagaagttg aacgtatctt
     3301 atatatggat tgcgacatga tcgctttaca agatatctct aaattatggc gtcttgactt
     3361 cggtgattca atcgtagcag ctgttgaaga cgctgggttc catcaacgac tggaaaaaat
     3421 gaaaatttca gctaaatcaa tgcgttattt caactcaggt ttgatgctga tcaatgtgaa
     3481 aaaatggtta gatgaaaata ttacgcaaaa agtattagat ttcatcgaac ataatccaga
     3541 aaaattacga ttccatgacc aagatgcatt aaatgctatc ttacatgacc gttggttacc
     3601 acttcatccg cgttggaatg cacaaggcta tatcatggca aaagcaaaaa aacatccaac
     3661 agcagcaggt gaacgtgaat acgaagaaac tcgcaacaat ccttacatta tccatttttc
     3721 aggacatgtg aaaccgtgga gcaaggattt tgaagggcca acgaagaaat attacgaaaa
     3781 atatgcaggg atgacggctt tccgttgtgt agctaaattc cctaaatatc caaaatatgc
     3841 aaaagttcaa ccaaaaacag atgtagttgt taaataatag aagacaacaa ctgaagaggg
     3901 ggagtaggaa tggaaattac aatgtctaaa aatgcagtag agacgttaat tgaaaaggtt
     3961 ggggaaaata caaaaatcgc actcgcgctt atcaatgatt ccgatccatt tctaagggac
     4021 aaaggtgctt ttgcaaaagg cagctttttt caaatcattc cttttgtttc ggaatttgga
     4081 gaatatgcga ctaaaatcga gcatccatta ttggacatct atacatctaa attagagcaa
     4141 aattatttcg gtaaacgatt gaacatggac ttcaacaaac agcttgacag cttttcatta
     4201 gaaaatgaaa tcgcagtact cgattataat atcaaattaa agaactgctt cttcagctaa
     4261 tagtttcgag aaagtccaaa aaggaggtat acattgagat actactttgt ggatgaagaa
     4321 aagaatgtaa tagaaacaga tgaaaaaaaa tataattggc tcgtacttga tagtgcaaag
     4381 cccgaagaga ttgagcaagc aatcgaagaa ttcgagcttc ctgatgatat tttcgtagga
     4441 accgaatatc cggaagaagt ttctcggatg gagcgccttt atgagacgaa actagagcat
     4501 ccattttctt tggtagtgat taatctagat cagtcagata atcgtatcga aaaaaaatta
     4561 acgccaatat cttttgtact ttccgataat ctcttgatta cttgtatcga tagaaaaacc
     4621 gaatttgtgg atcggctgct gacaaagcat ggaaatcatt taaactcgtt tgaaaagatc
     4681 atcacgtata cattattaga tatttacacc cattacgtga aagaactaag agagatgaaa
     4741 aagcgaattg acgctttgga taaagcagct cgtaaaacaa cggaaaatga agaactgtat
     4801 aaacaggcag atcttgaaag agacatcgta tatatcgatc ataccttaag agatcaacgg
     4861 gaaacaatgg atcaattatg gaattctgaa actttcatca aacgtttaga cgatgaacag
     4921 ctactctatg atgtccattt gagacagcga caaacagaaa aaatgatcga gatctatcga
     4981 gatttattag agagtattgg tgatctattc aacgggatga tggacaataa tctaaatcat
     5041 atcatgaaat atctagattc agctgcatta atcatttccg ttccggcgtt gttcgcaggg
     5101 atttggggaa tgaatacagg aggattaccg ggtaaaagtt ctgctctagg ctttatactc
     5161 gtagtctttg gctctttgat agcagcagta gttttaggtt accatttata ccggaaagat
     5221 tatacaaatt aaagaatcta tttaaaacag gtatcaaaca aagaaatttg ttttggcaat
     5281 acaatttaaa aactaggagg aatacagcaa tgacagaacc aaaattaatc gacccgcgaa
     5341 acttgtacca tacggagaaa tttcctaaac aagatcagga tacaccagct ttacagcaga
     5401 atatgattcc tgttcctgat tgtggagaag aatcctatga aaaggcaata atcaactaga
     5461 aaaccgccgt gtactaatca ctggtggaga ttcagggatc ggtcgtgcag cagcgattgc
     5521 atttgctaga gaaggggcag atatcgctct tcaattcttc ccaggagaag aaaaagatgc
     5581 agaggaagta gcaaaatacg tgaaagatgc aggacgtaag attgtcttac taccttttga
     5641 tcttcgtgac gaacaagcac ctaaagaaat catagataaa gccgtgagtg aacttggcgg
     5701 gctagatacg atggtactaa acgcaggaca gcaaatttct tgtcagtcta ttgctgagtt
     5761 accacttgag caagtcaaag acacatttat gataaacatc atcgctatgt ttgctttagt
     5821 gaaagaagct gttccgcata tcccagcagg aggagcaatc gtaacaacaa cttctgttca
     5881 agcattcaat ccaagcgaac atttattaga ttacgcagca acaaaagctt caatcgctaa
     5941 ctttacgatc ggtttagcaa aacagcttgc acctaaagga atccgtgtaa atggtgtagc
     6001 acctggtcca atttggacac cattacaact agacgaagga caaccttctg attcgatccc
     6061 tgaatttggt cagcaaagtt tactagaacg tgcaggtcag ccaggagaat tagcaccagt
     6121 ttatgttttc ttagcatcga ataaagcaag ttatgttaca gcccaaattt acggagtaac
     6181 tggtggagaa gcgatcaact tataagaagt tgatcgtttc acccctatga ggaggtgcga
     6241 aaatgaataa aggtgtaaaa acgatcggag tgatcggaac attacttttg ctatctgtgc
     6301 ttttgtttgc gatactatta agtgcaacct atccattgcc ttttcagcta gagaagtttc
     6361 gattcttttc catcactaat tcatacgtgc aacaatatgt attttggctt gccgttgcat
     6421 ttgctatttt gacaattatc acgttacttg ttttcctgtt ttatccaaaa acaatcggaa
     6481 actttctact gaaacatggt gatggggagt tgacgttgga taaaaaagca attgaaggaa
     6541 tggttcgttc tcacttgcat gaagatgaat tcatccattc accaaaagta aatgtcaaag
     6601 caacgaaaaa caagatcgcc gtcaatgtaa aaggggagtt gaaacgaact tcagcactca
     6661 ttggtaaaac aggaacattg atgaaagaaa tcgaaaatga agtacaacac atattgggaa
     6721 caaaagagcc agtgaaagta tcggtgaaat acacaggctt tgaagaacca gctaaacgaa
     6781 gcaatgaccg caaacgagta gaataggggg ggaccaaaaa tgaaagaact attttcgaca
     6841 tacaagctcc ctatcatctt tggggtactt ggattgatat tagccgtttt atggatcacc
     6901 gtgggctttt tcaagacctt attgatcttg atcatggcag cgatcggcgt cgctatcggt
     6961 ttttatctga aacaaacgaa aatcttagat cactatttta aatcttagga ggaaacatcc
     7021 atgaataaca ataacaaaaa taataacaat aaaatgaaca accaaaaacc agtgtctgca
     7081 catccagacc caacgacacc aaaccaatca aatggtgtga acgatgaaaa gaacaaaaca
     7141 gcaccagtat ctgcacatcc agatccagta gtccctaacg ttcaacaagg agtagaagag
     7201 aagaaacatg atgcagaaaa gcatgcacct catcatccag gaactccaga atctaaagtt
     7261 ccagaacaac atgatgcaat cgatccttct aaacctgtgg aagagcaaaa acatccttct
     7321 gaccatgcga aagcacaaga aaaacttagt tctgaccaca caaacggaca aggaaaactt
     7381 ggttctgatc atacaaataa ccaaggaaag catgattctg aacaggaaga ttcaatgcgc
     7441 ggtgagctga attatgaaga taaagttgtg caaaaaatca tcggaatcgc aattgaacaa
     7501 gtagacggtt tattatctgc aaacggtggt ttcttctcta atgtggcagg aaaacttgtg
     7561 aatacagata atgtaacggc tgggatcgaa acagaagtcg gtaaaaaaca agtagcagtt
     7621 gatatggatg tcattgtgga atatggcaaa gatatcgaaa aaatctttga agaaatgcaa
     7681 gaagtaatcg gcaaagaagt aaaaaatatg acacatttag aagtgatcga agtcaacgct
     7741 aacgtagtgg acatcaaaac aaaagaagaa tttgaaaaag atcaagaaac ggtgcaagac
     7801 aaagtaactg acgcagcgaa aaaaaccggt gaatttgctt cgaatcaaac agataaagta
     7861 aaatcagcag ctggaagcgg cgcacaaaaa gtaaaagaaa atacagaacc acgggttcag
     7921 taataaggaa gtaaaacatt aggaggaacg aaacatggga tttatttggt cacttatcgt
     7981 cggaggaatt atcggagcaa ttgcaggagc aatcacgaat aaaggttctt cgatggggat
     8041 tattgcaaat gtcgtagcag gtttagttgg atcagcactt ggtcaagcat tattaggttc
     8101 ttggggacca agtattgcag gaatggcaat cgtgccatca ttgattggtg ccatcatttt
     8161 agtactaatc gtatcattct ttgctcgaaa actgtaaaaa gacttttaaa aagaggtcgt
     8221 gaaaaagctg ttcttcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     8281 ttcatttggt tgggcatatt atcgaagatg caagcttttg aacactgttt atacggaata
     8341 agaggctgtg acaagacttt tgtcacagcc tcttatttat gcgggcaaat tcccaattag
     8401 atttttaaaa gtttttcctt aaatgtgtct ttattgatac agagaagctt ctttaataaa
     8461 acaatgcctc cctaaatttt cttgagatag gaaaggtgaa catacaaaat aatacgacca
     8521 ctttgaaaac ccagatgatc cacaaatgga tcgtatgata ctgatgaagt aaaaagcaat
     8581 ggatcagaaa ctcaaagaga ttgaaaaaga caaggaagaa ataatatctc ttttttactc
     8641 ttgtctgttt tagccaataa tagctcgtaa agtaatctag acaaacaaat agaagtaaac
     8701 aaagtttcgt aagagcagca atcgtatcat gttgctctgc tagacgattg acgagcagga
     8761 aaaatgttgg taatggaata tagatcacga gtaatgaaag aaaaaagtaa acaataaatg
     8821 gcatactatt cctcctaatt gtgtgagttc gccctcattt taaaataacg atatttgttt
     8881 gtgaagcttt ttcgtgtcgt aacaaatgtt atctaatgta tttttaaata aataatcatt
     8941 attatcaaaa taatg
//
LOCUS       DAYYGY010000101         8942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000101 DAYYGY010000000
VERSION     DAYYGY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.103"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACHA52_002555"
     CDS             complement(15..1154)
                     /locus_tag="ACHA52_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8941215.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACHA52_002556"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACHA52_002556"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8941216.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACHA52_002557"
     CDS             complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACHA52_002557"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8941217.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACHA52_002558"
     CDS             complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACHA52_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8941218.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACHA52_002559"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACHA52_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8941219.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="ACHA52_002560"
     CDS             complement(6168..7259)
                     /locus_tag="ACHA52_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8941220.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACHA52_002561"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACHA52_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8941221.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="ACHA52_002562"
     tRNA            8802..8873
                     /locus_tag="ACHA52_002562"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3081 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc agacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaagtctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aa
//
LOCUS       DAYYGY010000102         8718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000102 DAYYGY010000000
VERSION     DAYYGY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.104"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(118..411)
                     /locus_tag="ACHA52_002563"
     CDS             complement(118..411)
                     /locus_tag="ACHA52_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8941222.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(439..1104)
                     /locus_tag="ACHA52_002564"
     CDS             complement(439..1104)
                     /locus_tag="ACHA52_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8941223.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1244..1876)
                     /locus_tag="ACHA52_002565"
     CDS             complement(1244..1876)
                     /locus_tag="ACHA52_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8941224.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1883..2950)
                     /gene="liaS"
                     /locus_tag="ACHA52_002566"
     CDS             complement(1883..2950)
                     /gene="liaS"
                     /locus_tag="ACHA52_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8941225.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(2947..3678)
                     /gene="liaF"
                     /locus_tag="ACHA52_002567"
     CDS             complement(2947..3678)
                     /gene="liaF"
                     /locus_tag="ACHA52_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8941226.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(3846..4325)
                     /gene="greA"
                     /locus_tag="ACHA52_002568"
     CDS             complement(3846..4325)
                     /gene="greA"
                     /locus_tag="ACHA52_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8941227.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4461..5636)
                     /gene="mltG"
                     /locus_tag="ACHA52_002569"
     CDS             complement(4461..5636)
                     /gene="mltG"
                     /locus_tag="ACHA52_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8941228.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5801..6961
                     /locus_tag="ACHA52_002570"
     CDS             5801..6961
                     /locus_tag="ACHA52_002570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8941229.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(7001..7948)
                     /locus_tag="ACHA52_002571"
     CDS             complement(7001..7948)
                     /locus_tag="ACHA52_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8941230.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7981..>8718)
                     /locus_tag="ACHA52_002572"
     CDS             complement(7981..>8718)
                     /locus_tag="ACHA52_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8941231.1"
                     /translation="TYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLIL
                     FALFNATKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAEN
                     TDISNARITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNV
                     LTSTGILGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNEL
                     VYENTVGTFVFWLFLGRVNSKNNSSYF"
BASE COUNT         2639 a         1797 c         1349 g         2933 t
ORIGIN      
        1 ccacccatga gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa
       61 caatattttc aaaagaaaaa agagaaggtg acaaaaattg tgtcaccctc tcttttttta
      121 ttgtttttta aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc
      181 atatccttta aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catccatttc
      241 cactttcttt acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg
      301 aacctctgtt ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc
      361 aatttctgag tcaaaccact tttgtgcttt tggcgttacc gttaatttca tcccaacaac
      421 ctactttctc ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt
      481 ctctccaata accagtagat tatcattttc acgaacatat tcatctgctg caggagaagc
      541 gataaccgtt tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa
      601 atttaattca gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa
      661 agaatattta tctgacagct ctaagtagtc tagaatattc tttgatacta agttatgtgc
      721 aatccgtatc cccatgtctc gctcagggtg tacgacacga tctgctccga tcttctctag
      781 tacacgggca tggtactcat tttgggcttt tgctaaaata ttcggtacgc ccatttcctt
      841 taccatcaga gtaactaaga tgctggcttg gatatcttct ccaatagcga caattacatg
      901 atcgaaattt cttataccta atgaacgaag tgtcatctca tcctgtgcat ttgccacaac
      961 agcatgggtg gctatattca tatattcatt gactcgatct tcattactgt caatagccag
     1021 tacttcttgt ccagcttcga tcagtgtacg gcagatgctt ccgccaaatc gtccaaggcc
     1081 tacaattgca aaattttgtt tcaccatttt cctcctttag tatcttcttt tctcagtata
     1141 acaaagcctt tccaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa
     1201 tagttatttt ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat
     1261 gtttaaacgc atagatagct gcttgagttc ggtcttctac ctctaactta gctaatatat
     1321 tcgacacatg cgttttgact gttttcagtg tgataaacaa ttcatccgcg atttcctgat
     1381 tacttttccc ttcagaaatc agcatcaaaa tttcattttc tcgattcgtt aattcttcat
     1441 gcaatactgg atcatttcgc cgactcattt tttccatcat ttttgttgtc acttcaggtt
     1501 ctagtactcg ctcgccgcgt tgcgtagccc ttatagcatc agctatttca tgtgctgtag
     1561 acgtcttcaa caaatatcct gctgctcctg cttcaattgc aggataaact ttttcatcat
     1621 caataaaact tgtgacgata atgattttgg cttttggcca atcttttaag attgcttttg
     1681 ttgattcgat gccatccatt tcttccatga ctagatccat taaaatgaca tccggtcgaa
     1741 gtgccatcgc cttttcatag ccttgacgtc cattttcagc ttctccaatc acttcaatgt
     1801 cttcttggat agataaataa gaagagacgc ctaagcgtac catttcatgg tcatctacta
     1861 ataaaacttt tatcatcttt atctatgcct ccttcatcaa agggaccttg gtctccacgc
     1921 tcgtaccttg ccctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt
     1981 ctttgatatt gttcaatcca taacttcccg ttttggtttc attcatatta aatcctgtcc
     2041 cgtcgtcaat gatacgtaat aaaagattgt tatctatttt atgcaaatag acttctaatt
     2101 cattagcctt agcatgtctt aatgtgtttg ataacaattc ttgaacgatt cggaacaagt
     2161 gatcctcaat ggaactagtt aattttacat cttctacctc ccattttagc gaaatctgga
     2221 ttttattctg caattccttc aataactgct caatgccttg ttttaaactt ttttcttcta
     2281 aattgactgg acgcagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga
     2341 tctctgccac cattttcaac tgcttacgga acatttctgg tgtctcactt ttttctgctt
     2401 gctccgtcaa tgccgacatc atcatcatcg ctgcaaataa ttgctgtgaa actgaatcat
     2461 gaagctcacg agctaatcga tgcctttcta attttaaaat ctcttctttg gtttgtccat
     2521 ctacatattg aggacggcta gtcataatct gtaattcact ggatacttcg atcatttttt
     2581 cttttatttt agtaatttcc atatcaatat ctttgatgta cagatcatcc gacgcgcttg
     2641 ggatcggctg atgcaaaagt ttggattcat aattgccaga agaaagtgca cgaagctttt
     2701 cttcgatttt cccgtatttt gccttttgaa taagtgataa caatagaaat acgatcaatc
     2761 ctacacctaa tgaaattgcc atcaaatgga agataagcgg aacgtataat aaacgcgcac
     2821 gcagcaattc tccccaccaa tgactttggt tactggcata aaaataagtg aaaagtgaaa
     2881 atagaataat aaggaaagcc gcaatccccg aataaacagc tagcattgct ttggatattc
     2941 ttcccatcat attcgtatca cctcgatatc tccaaccaaa gtattggtaa tgatcttcaa
     3001 tctgcgagga ttttcatcat aatcactgct gcagattttg atctgttcgt tttttaagga
     3061 aaactgctct tcttcaaaca agacatttcc tactaatgtc gcatgctcta aacgaatagc
     3121 cactcccagg ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt
     3181 atcatctttt ggcaaaagtg tattgcccaa atcgatgata gtatcaccag aaatgatggc
     3241 aatgttgata tcatcccatt catagacgtc gtttccaata cgttggttac caaataattg
     3301 ctgtctttta cgctcattat tatgtgtttt taattgctct gtttggacca tcatgatctg
     3361 tttttttcgc caaaaagcat tttttgtcaa atctattcct gaaatctcta cacctttcag
     3421 cccgataaat aaaacggcca atacaaccat catccataaa gcaggactgt tgactaagct
     3481 aaaaaaaatg accaagctcc ctaagatcaa ttgaaaattt tgaaatttag tacgaggata
     3541 tttgcgcata gctagatata tattgaaaat accgaatatt actaaaagca aaagtccagt
     3601 attgtttacg atttgccaaa cagcaaataa cagcagcagt gcttcgacta ccacaaaaaa
     3661 acgccaagaa ctattcatcg ctttttctcc ctcctgacac attctcattt catcatacct
     3721 atttttttga ctgttggcta taggacttta gatgtaagat cgttcagtga aacctgcaat
     3781 actcaaagag aaaagatcta gagtgcgtca tcagtaagat gaactctcta gatctttcag
     3841 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggtgtagca
     3901 atggttactt catcattttt acgtttgcca atcaatgctt gcgcgatagg agaatcattg
     3961 gaaatctttc cagaaaacgg gtctgcttca gcacttccga caatcgtgta ctcttcttca
     4021 tctccatcag gcaactcgat gaacgtaact gttttaccga tcgatacctc atctgcatct
     4081 acgccgtcat tatcgatgat ttgtgcaaaa cggatcatat tttcaattgt tgtgattctt
     4141 ccttcaacaa atgcttgttc atctttagct gattcgtatt cggagttttc ggaaagatct
     4201 ccgaaaccgc gagcgatttt gattcgttca atgatttctc cgcgtttaac tgtttttagt
     4261 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4321 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4381 atagtccaaa aaagattacc acgcttttct taaaatgtaa atgtttttct aaaaatgatc
     4441 aatgactagc ccctgttttt ctaagaatta tttacatatt tttgcactag ttccatatgt
     4501 tcttcatacg ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4561 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4621 ggaccaggcc catagccggt attggtatac aaattgtatg gtgaatctac agctgtatct
     4681 tcatacgtca ccaattcttt atgttctcct aatgcatata aaacagagat atctgactga
     4741 agtggcatat ttgccttgat tcggttaaag aagacttgtg caatattttt acgatcattt
     4801 tcttttacgc cttctttttc aactagagaa gccaaagtta aaacttcttg tacagtcaaa
     4861 tttttctgtt tgatagctga ataatagttt tgcatgaccg tatttgtttt gttcaccatc
     4921 tcaatcacaa gatccttaag cgaagtactc gtgtagtaat cataagtagc tgggaataag
     4981 tagccttcca aacggtattt tgtgttctca gctttagagg cactttccaa caattctggg
     5041 aatttttgat acaactcgtt aaagaaagtc tcatcattta ctaaatctaa aaattctttc
     5101 ttgtctttac cagttacctt cgctacccgc tcagcaatct gatcgatgtc gtagccttct
     5161 ggaatggcaa ttttcccatc tgcaactttt gttggctcag atgtgccgcc ttcttgcaac
     5221 tgcttgccaa tctcatcaag agtcatatta ggcgctaact gataataacc agcttgaaag
     5281 ccagttaagt ttttaaattt tgtataataa ttgaaaacaa tcccactttt aataatattg
     5341 tctttctcca aaatttcgcc aatctgctta ttagaagagc cgcttgggat ttccacttgg
     5401 actaattggt cgtccgattt atctaaaggc ttcaacccgc tatctacata acgatatacg
     5461 gtaaacccga gaaatccgcc aataatcaat aatgtaagag cgatcactaa aatgatcttc
     5521 ctcacgatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5581 gctgccttac tggcttcttt tttgtctttg cttgggtgtt gtttgtttcc tgacaaaaca
     5641 actcctcctt actatcatct tcgtaactct gtttattata catgaaattt ctactgccgt
     5701 gaattatttt accaaatatt atgtcaaaat tgtaagtggc aaatgctctt tttgaggatt
     5761 acaatgaaaa atagttaaaa atggatagga aggatttaat atggcaaaag gaaaacatca
     5821 aaaaaaacag cagatctctc tttcttttct cgcagttttg ttgctggctg tcagcttctt
     5881 ttcaacaggc atcattacag gatggcaatt aacaaaacaa cctcaaccac aaatttataa
     5941 aacggaaccc gcacaacttg cttccaattt aacatctaat gagcagatgc ttgccagcaa
     6001 aattgatcaa ttactgcaaa aatcagatta tatcggttcg atttatgtac gcaaaaataa
     6061 tcgagtgata ttagaaaaag gatatgggta cgcaaatcta cgcataaaag aatcgaattc
     6121 tccttctctt tactatcaaa tcggttcgat ccaaaaagcg atgactgctt tacttatcct
     6181 aaaacaaatt caatctggca agataacttt tgacacaaag ctatctgaat tttatcctca
     6241 gattcctgga agtcagcaaa tctcaatcaa aaatatgctt tacatgcgct ctggtcttca
     6301 tcgaaccgct tctccaaaaa aacctatgtc agatagtgag atcattcagt ttgcccttca
     6361 tcatttgaaa tttactggtt atgatacgta tcattatgaa ccattaaatt atacattgct
     6421 tacaggggta ttgatcaaac taatgaatca gccttatgaa actcttttaa aaaaagaaat
     6481 cattcggcca ctccatctag aacatacaga tttttatgaa aatgtaaaaa attcccctca
     6541 gcatgctgta tcttaccaaa tgtctgcaac tgacgattat tcaaaagcat tggtcgagcc
     6601 tgaaacagac atccgaaatg aattaggcac aggaaatata agtatgtctg tttatgactt
     6661 gaacaaattc tttaccagtg ttctgtctgg cgatattata ccaaaagaac tacttttctc
     6721 attgtggcaa aataatggtg ataaacatcc ttacagcggc ggtgtttaca gtggaaatga
     6781 ttatatcctt gctcagggaa atatcaatcg attccatgca gtagcggcga tgaaaaaaga
     6841 cacaaaagat gcaatcgtca tggaaagtaa tatccaatct gataaaaaca tcaaattgcc
     6901 ggccactgat ttaagaaatc agatttacga actgatggaa ggtgttaatt taaaaagcta
     6961 agtcgagaca aaaaaaccag caggatgctg gttttttgtt tcaatcgatt tctgtcttta
     7021 aataacgttc cattccgttc aatatatcaa atttgggttg ataacctaaa gaacggatct
     7081 ttgaaatatc cgctaaagaa tcacgaatat cccctgaacg ctccgcttca tatttaagcg
     7141 ctagctctgt tcctaagatt tgatcaattg aatgaatcaa ttcaagtagc gtagtgcttt
     7201 ttcctgtacc gacattgaat tgctgtccta aagctttttc ttccttagca acaagtaaaa
     7261 gtgcttgtat aacatcttct atataaacga agtctctaga ttggctacca tcaccaaata
     7321 aagtaaattc tgttttttct cctgccaatt gttttttgta tcggtccact agaatcgaaa
     7381 taactccgct atatggagag ttaggatttt gatttggacc ataaacgtta aaaaatcgga
     7441 cagcactagt cggaacatca tataaatgac agtaatccaa aacatattgt tctgctgcaa
     7501 acttatcgat ggcatatggt gttaaaggac ggatgaccga ttcttctttt tttggcaacg
     7561 ttggttcatc gccataaact gcagctgatg aactaaatac caatcgtttt aaaccagatt
     7621 gatattttcg taccaattca agcaataaaa gcgcactgtc aaaatttaca cgatgagttt
     7681 ctagaggacg ttctacagaa tctgccacac tagctacagc agctagatgg aaaatataat
     7741 cgaaatggta ttctttcatg accttttcca tcaaattttt gtcagcgaca tccccttcaa
     7801 taaacgtgat gtgcttcgat tcatccagat tctctttttt ccccatcgat aaatcatcga
     7861 tcacaactac attgttttct tttcctagat aattcgcgag cgtcgagcca atgaagccgg
     7921 cgccaccggt aatcaagtaa ttcaacattt tttctcctct tttcaagata aaatttatta
     7981 ctaaaaatag ctagaattat ttttgctatt aactcttcct aaaaatagcc aaaaaacaaa
     8041 cgttcctaca gtgttttcat agactaattc attgttaaga gctgcgtata gtgcataaga
     8101 taaaataact aaacagtaaa caaaatgata atctttacca atgcatttat tatagatgat
     8161 tcgataaaca ttcagcacaa aactctttat taaaaaagcc atgaaaggaa ttattcctag
     8221 aatacctgtc gatgttaata cattcaagta tgcattatga atggcaattc ctactttcgc
     8281 aatatatcca tctggaataa ttgactgtgc ataagatact acatttttcg gagagactcc
     8341 gataagccaa cttgttttaa atatttccca tgcacttttc catatagtaa ttctagcatt
     8401 agaaatatct gtattttcag ctacgtcttt cctaacaaga gtattatcag gtgtttgagt
     8461 attattaata tgttgactat tattcattaa ttctttatga cgctctgtaa aattaaaaaa
     8521 atttatttct tttaaatgag gtaataattc ttgaaaaagt atcttagttg cattaaacaa
     8581 agcgaataaa atcagaacag aaccaataga agatactata gcaaggatat agttttttaa
     8641 atcttttttt gttccatttc gttttaacac aacaataaaa gatgatatgg caactattat
     8701 tatacttacc acataggt
//
LOCUS       DAYYGY010000103         8715 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000103 DAYYGY010000000
VERSION     DAYYGY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.105"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..653
                     /locus_tag="ACHA52_002573"
     CDS             57..653
                     /locus_tag="ACHA52_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM8941232.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            634..1080
                     /locus_tag="ACHA52_002574"
     CDS             634..1080
                     /locus_tag="ACHA52_002574"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8941233.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1423..1746
                     /gene="relB"
                     /locus_tag="ACHA52_002575"
     CDS             1423..1746
                     /gene="relB"
                     /locus_tag="ACHA52_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8941234.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2066..2293
                     /locus_tag="ACHA52_002576"
     CDS             2066..2293
                     /locus_tag="ACHA52_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8941235.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2309..3475
                     /gene="hemW"
                     /locus_tag="ACHA52_002577"
     CDS             2309..3475
                     /gene="hemW"
                     /locus_tag="ACHA52_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8941236.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3710..4753
                     /gene="hrcA"
                     /locus_tag="ACHA52_002578"
     CDS             3710..4753
                     /gene="hrcA"
                     /locus_tag="ACHA52_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8941237.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4847..5410
                     /gene="grpE"
                     /locus_tag="ACHA52_002579"
     CDS             4847..5410
                     /gene="grpE"
                     /locus_tag="ACHA52_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8941238.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5461..7290
                     /gene="dnaK"
                     /locus_tag="ACHA52_002580"
     CDS             5461..7290
                     /gene="dnaK"
                     /locus_tag="ACHA52_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8941239.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7441..8607
                     /gene="dnaJ"
                     /locus_tag="ACHA52_002581"
     CDS             7441..8607
                     /gene="dnaJ"
                     /locus_tag="ACHA52_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8941240.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3051 a         1419 c         1877 g         2368 t
ORIGIN      
        1 cgatttttca agttatctaa tgcaattata ttgatatcat taggagctgc attgttatga
       61 atccaacatt gattactaag aaagaattac taaaaaaatt agatatttct actggtgtat
      121 tagctaattt aattagaaat ggcatgccaa aagagggaga aatgtttaat cttgataaga
      181 ttattacatg gcgagaaaat tggtctaaaa acatattagg tgagttagaa gttggtagag
      241 tatatactaa caaagaaatt tcagagaagt ttaaatgttc caaacagggt ggaatgagaa
      301 gatctcatca aactaatact cttgtactgt ttagtgatca aacaggaagt aatgtttata
      361 aagataagtg gttaaatggt attttacaat ataccggtat ggggctaaaa ggggatcaag
      421 tattggataa gaatcaaaat aaagttttag ccaattcaaa aagtaatttt gtaaagattc
      481 atttatttga aacatttaaa ccaaaagaac acacatatct gggagaagta tatttagcag
      541 gacaaatata tactgttaat gaaaaagata gttcgggcaa cagcagaaaa gtatataaat
      601 ttccattagc tttaataaat caagagcaat tgattgaaga taaagatata taaccaggaa
      661 gaaaatcaaa ctagacatat tcgtaattta tctaatgcta aattagagga agaagcaaga
      721 aaagttagta attataataa aaaaatggct gcaaaatatg ttaacgaaga ggagttttgc
      781 ttctcaaaaa caaaaatata taaacgtaat cccttcgtat ctgaatatgt taaacgactt
      841 gcaaaaggta tttgtcaact ttgccaagaa aaagggccat ttataaaaga cggtgtacct
      901 tatttacatt gtcatcatat agaatatttg tcgcaaggtg gaaaagatgt gattgaaaac
      961 tgtatcgcgt tatgtcccaa ttgtcatgct agaattcatg aacttgagtt acctgacgat
     1021 aaagaaaaat tgctgaagat cgttgaaaaa agagaaaata gcttttctaa aataccgtaa
     1081 tttattttta taggacaaat aagctaatgt gagctatgcc ttctatataa gactaagttc
     1141 tattataagt aacgaagtaa ttctcgttcc ttcctgtagt aatatttttt aaataaccta
     1201 taggcttctt caaaagtaca tcctccagta aattctaagt actggaggat tctttttcta
     1261 gtgttgcttt actcatgaaa aagtagttgg ctacttcatc aaaatttctg aactttttta
     1321 atgtataata aagagctcga tcatttacta caaggatgct agctcctaca ttttcacagt
     1381 tttttagcaa gtaaattttt ctaaaatcac atcaggaaat gcatgagatt ctttaagaga
     1441 ggcaattata gggctatgat tacggttcgt gtaagtgacg ccaaaaaaga atggctttct
     1501 tatatggctg aattttatgg gattacttta tctgaacttc taaaaaacta ttcgatggaa
     1561 caactagaag atgaatacaa tcgtcaaacg gctgaaattg cccataaaca ttggataaga
     1621 agtgggaaaa caacagtatt catggaagaa tccttaatga atttggtggg ctggaatgac
     1681 ctgccgttta aaattcacgc ccgaaattca gaagcagctc tgattggcaa atacgtgtat
     1741 tggtagagat agaagataat caattcgttg ttatcgctat tcaagtaggg caccgaagaa
     1801 atgtctatga ttaagagact gtttattttt acaaaaaaat aataattgga atctagttaa
     1861 aactcttgaa gtgagagatt tgactagatt tttttatgaa taaaataagg acaaaattgt
     1921 ttaggaagaa tcatgagtgt aaaaaaatca tccaagaagt cgattcttgg attttttagt
     1981 ggatttgttc gccaaatacg tttagtatgc gtatatgaga ggttgtaagc aaagcgttgt
     2041 ggtctgagca cttaaggagg aatctgtgaa aatagctttt catatttttg cagattttga
     2101 cttaatgccg aaggtcagct ttgcgattac cgtttatcga gaggctgtaa aagcaccgtg
     2161 tagctccgat gacaataagc aagcattttt agagctaggt ttccttgatt ctaaatatgc
     2221 ggcttatttc aatgagttgg tgcttgaacg cgattttaag aaggtcgaaa acaggcacga
     2281 cttttcgacg taaggagaaa gaatttcaat gatagaaaca ataaatcccg cagaaaacaa
     2341 ggtttctgcg tacattcata tcccattttg cgagcatatc tgctattact gtgactttaa
     2401 taaagttttt ttagaagggc agccagtaga tgagtatgtg gaaatgctgc tgaatgaaat
     2461 gagattaatg ctggaaagtc atcctacagc gaatttggaa acactttatg taggaggcgg
     2521 aacaccaact tctttatctg ctaaacaact tgatcgacta ttatcaggtg ctagagagat
     2581 cctgcctttt caaaatggta atgagtttac tgtagaagcg aatcctggtg atttgacccg
     2641 tgaaaaactt caagtgatga aaaactatgg cgtcaatcgt ttatccatgg gagtgcagac
     2701 atttgacaat cgcttattga aaaaaattgg acgtaaacat actgcagaag atgtgtatca
     2761 aacaatgcaa tttttagaag cagaaaattt taccaatgta agcatcgatt tgatttatgc
     2821 attaccggga caaacactag aaggatatcg ggatactttg gaaaaagctt tggctttgga
     2881 tctgcctcac tattctttgt actcattgat cctcgaaaac aaaacaatgt tcatgaactg
     2941 ggtacgacaa ggaagactcg aactgcctga tcaggaaacg gaaaccagaa tgtttgaaga
     3001 gacgattgaa gcaatggaaa agcatggacg tcaccaatat gaaataagta actttgggct
     3061 ctccggacat gaatctcaac ataacctcat gtactggaac aatgaccact attttgggtt
     3121 tggagctggt gctagcggtt atcttggaga tattcgttac cgcaacaaag gaccaatcca
     3181 acactattta cgcccattac gagcaaatga acgcccggta ttggaagaag aaaaattgac
     3241 acgaaaaaat caaatagaag aagaaatgtt cttaggacta agaaaaaaaa cagggattac
     3301 taaaaaacat ttctatgatc gctatcaaaa aacaattgaa tcggtgtacg gtgatgtgct
     3361 aagggaatta gagcaagaag ggttacttgt taatgaagaa gaccgtgttt atttgacacc
     3421 aagagggact tttttgggga acaatgtgtt tgagcgcttt ttatttgaaa aatagtttga
     3481 agaatatttg ttttttatcg aaacttgaaa agaaaaagtt tagaaagttt gacaatgagg
     3541 tattgaagta ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt
     3601 tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat
     3661 aagtgaatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa
     3721 gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt
     3781 tggttccaaa acattaatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga
     3841 tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat
     3901 tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc
     3961 agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga
     4021 gattattaag caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt
     4081 gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg
     4141 acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct
     4201 tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct
     4261 agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct
     4321 tcataaatat ttccagacga cagaagggat gtctcagtta tttgacaccg ttcttggtca
     4381 tgtttttgaa gaaaaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg
     4441 tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac
     4501 acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa
     4561 tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag
     4621 aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt
     4681 cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgct cgcttgactt
     4741 gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt
     4801 aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca
     4861 agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc
     4921 tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa
     4981 agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat
     5041 ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc
     5101 caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga
     5161 tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc
     5221 actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct
     5281 tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga
     5341 agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacta tggtcattgt
     5401 tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt
     5461 atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa
     5521 ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta
     5581 tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac
     5641 ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta
     5701 gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga
     5761 ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac
     5821 tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta
     5881 gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat
     5941 cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt
     6001 gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg
     6061 gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc
     6121 attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg
     6181 aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga
     6241 gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca
     6301 gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgttaaaaga tgcaggtctg
     6361 tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt
     6421 gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa
     6481 gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt
     6541 gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact
     6601 aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg
     6661 gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct
     6721 gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt
     6781 ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa
     6841 gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac
     6901 gaagaaatcg aacgtatggt gaaagatgca gaagcaaacg ctgaagcaga taaagctcgt
     6961 aaagaggaag ttgacctacg taatgatgta gacgcattgc tattctctgt ggacaaaaca
     7021 ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgt
     7081 gacgaattaa aagccgctgt tgaagcaaac aacatcgagg aaatgaaaac taaacgcgat
     7141 gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc tgcacaacaa
     7201 caagctcaag aaaatccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat
     7261 tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa
     7321 agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa
     7381 caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct
     7441 atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat
     7501 gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa
     7561 cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg
     7621 caaaaacgcg ctgcatacga ccaatacggg catgcaggca cagatccaaa ttacggtgct
     7681 ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt
     7741 tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg
     7801 cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt
     7861 ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtggaaac
     7921 ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatc
     7981 aatgtagaac gccaaacgcc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt
     8041 catggcacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag
     8101 aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg
     8161 cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg
     8221 gtttttcgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa
     8281 ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat
     8341 ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt
     8401 ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa
     8461 ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa
     8521 gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat
     8581 gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aatataaagt agacttgccc
     8641 aaattttcta tttctagtaa aaacgctaag tcattaataa ttaaaaacca gacgaacaat
     8701 catacgttcg cctgg
//
LOCUS       DAYYGY010000104         8530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000104 DAYYGY010000000
VERSION     DAYYGY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.106"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..763)
                     /locus_tag="ACHA52_002582"
     CDS             complement(134..763)
                     /locus_tag="ACHA52_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8941241.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(756..1874)
                     /locus_tag="ACHA52_002583"
     CDS             complement(756..1874)
                     /locus_tag="ACHA52_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8941242.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1876..2508)
                     /locus_tag="ACHA52_002584"
     CDS             complement(1876..2508)
                     /locus_tag="ACHA52_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8941243.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2519..4336)
                     /locus_tag="ACHA52_002585"
     CDS             complement(2519..4336)
                     /locus_tag="ACHA52_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8941244.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4349..5113)
                     /locus_tag="ACHA52_002586"
     CDS             complement(4349..5113)
                     /locus_tag="ACHA52_002586"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8941245.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5141..5839)
                     /locus_tag="ACHA52_002587"
     CDS             complement(5141..5839)
                     /locus_tag="ACHA52_002587"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8941246.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5851..6630)
                     /locus_tag="ACHA52_002588"
     CDS             complement(5851..6630)
                     /locus_tag="ACHA52_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8941247.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6646..7590)
                     /locus_tag="ACHA52_002589"
     CDS             complement(6646..7590)
                     /locus_tag="ACHA52_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8941248.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7636..8415)
                     /locus_tag="ACHA52_002590"
     CDS             complement(7636..8415)
                     /locus_tag="ACHA52_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941249.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2297 a         1769 c         1452 g         3012 t
ORIGIN      
        1 ttgttttctt atatgtgagt aatcagatac aacattaaaa tattcgatac tgttttccgt
       61 cagaatttcc tctaatatat tcgttcgact gatataatca aaaggatgaa taagcagcca
      121 ctttttttta ttctcactca tctatcttcg caccctcaaa tttctcagta gtcccacttg
      181 ctactgatgt tattccatca cggacaataa ctttctttag cgttaaaaaa aatattttta
      241 tgtctttcat cattgaaatg ttctccacat attcaatatc tttttcaaat ttttctttcc
      301 aactaattgc attacgacca ctgacttgag ctaatccggt taaacctggg cgcacaaaat
      361 gtctttttcg ttgttcatta ctgtatagtg aaagatactc aactaacaga ggacgcggtc
      421 caataatact catatctcct tttaaaatat tccataattc tggtagttca tcaagactag
      481 tcgaacgcaa tattttacca aattgagtca atctgatatc gtctgataat aaattacctt
      541 cttcatctct ttgatctgtc attgttcgaa atttatacat cagaaaaatc ttttcatgtt
      601 ttccaggtcg ttgttgttta aagatcactg gcgatccaag ctttattcga actaggattg
      661 caacaaacgt catcagtggc cataacatta ttaaacctaa aattgaaaaa agaatatcta
      721 tcaaacgttt aaagaacttt tcgtagatac ctctattaga cattttgcat cactcgctta
      781 attgtattag ttactcgata caaatcatca tcggtcattt tagaatctga aggtaaacag
      841 ataccttttt caaacaatgt ctgagcaaca tctgtaccaa tatactcata cttatcgaaa
      901 actggctgta aatgcattgg tttccatatc gggcgggctt ctatattatt tttatctaac
      961 tcatcaataa gatgattcgt atttattccc tttactgtta tgacacttaa ccaataattt
     1021 ggttcattcc aattattttc tggcatgaat tgtatatttg acaatttaga caattctttt
     1081 ttataaaaac tatgaatata tcgttttttc tcaattcttt catttaaaac tttcaattgt
     1141 cctcgaccga tacctgcaac gatattgctc attcgataat tatagccaat ttcattgtgt
     1201 tgataatggc gtgccttctc acgagattgc gtagcccaaa acctcacttt ttccaccttt
     1261 tctttgtcat tagacacaag cattccgcca cctgaagtcg tgattatctt atttccatta
     1321 aaagaataaa ttccatagtc gccgaatgta ccagtatatt ttcctttata tgttgttcct
     1381 aatgattctg cagcatcttc aattaaaaca acattatgtt ctttgcatat tttagtaatt
     1441 tcatctagtt cagctgataa accgtacaaa tgcactacta atacagcttt tgcgttagga
     1501 tatttttcta gcgctgttcg caaatgttca gggttcatat tccaagtttc gtaatcacta
     1561 tcaacaaata ctggaatagc tccttgatag atgattggat tggcagtagc tgagaacgtc
     1621 aaagactgac aaatcactat atcatcttta ccgaccccag ccgcttttaa agctagatgt
     1681 atagctgctg ttccagaaga tagtgcagct gcatgactag caccgacata ttctgctaat
     1741 tcctcttcaa aaccattgac attcataccc aaaggagcca cccaatttgt atcaaatgct
     1801 tcttttacat actcttgttc atatccttca tcactcatat gaggagatgc taataatatt
     1861 cgtttatttt ccaacttaat cccatctttc tatgactttg gtaggtacgc caacattttt
     1921 agtcttatta ttaatatttt ttattactac acttcccgca cctataatgt tcatatttcc
     1981 aattcttaga ttttgtatta ttttagtacc tactccaatt tcatttttat ctcctaatag
     2041 aacatgtcct gaaatattta cacttggaaa aattgaatta taattaccga tttgagtatc
     2101 atgtccgata gtagagccga tattgatcat attgaaattt cctaaaacta cgtcagcagt
     2161 gtaagtcgta tagggcatca aaatattgcc aattcctaac tgaacattta tgcccactag
     2221 tgcacttgat gcaactaaat tagggaaaat caatttatta tttttattta tcttttgata
     2281 tattttctct ttgatatctg gcgaagcgat tcccagaaaa atagcagttt cttcttctaa
     2341 ttctaataac gagtctattg ttcccaatac agtttttccc actaattctt gattttgcat
     2401 ttttaacgaa tcatcgacaa aaccaatcaa ttcatatcta gagaaacttt ctagtagaaa
     2461 agcaatctct ttgccaaatc caccagctcc tacaataatt gctttcactt tactccccct
     2521 acttattcga agcattcgca aaagtgacaa tgtctttagc caattgttca tcgtcttgcg
     2581 gcaggctctt gacaaattcc atcactgttt gcatggaata acctttgatg tttcccacaa
     2641 agatcttctc gtaaactgct tcatcgtttc tttccttatc taacaatagt tcttcatata
     2701 gtttttcccc tggacggata cctgtttcaa tgatttcgat ctcgtcttcg ctatatccgc
     2761 tcaatcggat catgtttttt gctagatcta cgattttcac tggttcactc atatctaaaa
     2821 taaagatttc gccgcctttt gctaacgccc cagattgaat aactaaacga cttgcttcag
     2881 gaatcgtcat gaaatatcgg gtcatacgaa agtctgttac agtgactggc cctccttgag
     2941 caatttgttc ccggaatact ggaatcacac taccgcgaga accaaggaca ttcccaaatc
     3001 tgacagctga gaatttcgta catccttctt cattcaaccc agtcacgatc atttcagcga
     3061 ttcgttttgt tgaacccatg acgtttggcg gattgttggc tttatctgtc gagaccatga
     3121 cgaagttttt tactttcgct gctttggctg cttctgctac attttttgta ccgaaaatat
     3181 tgtttttgac tgcttctttt gggttatatt ccatcaatgg tacatgctta tgagcggctg
     3241 catggtagac gatatctggg tggtattgtt ccatgatctc attgattttc ttgcgatctt
     3301 ggatatccgc aatgatcggc acgatttctg taggacactt ttgatgatgc gtgcgtaatt
     3361 cacggtcaat caagtaaatc gagttctccc catgacctaa aagtaatagt tttgcagggt
     3421 caaattggat gatttgccgg cagatttctg aaccgattga tcctcctgca ccggtgacaa
     3481 gaatgacttt gtttgtgatc tggtctttga tggattcgat atccaactct acctcatctc
     3541 ttcccagtaa ttccactacg tcgatggttt ttaattttga tacgctgatt ttccctgagg
     3601 caagttcttc catagaaggc atcgtattga ctttcacatg agcagattcg actaactcga
     3661 agattcttcg taattttttt cttgataaag aagggatagc gatcgttacc atttggatgt
     3721 catatttttc gatcaattcc ggtatatctt ttaatgaccc cagtactttt tttcctgaaa
     3781 gataggtatt tcttttattc ggatcatcat ccacgaatcc aatgacatgg atatcttgag
     3841 ccgttttaga accaagaaag ctgttgtata aaatacgtcc accttcgccg gccccaacga
     3901 tcaaggtatt tttagcggaa tctgttgata gatacctcat attttttgtt tcgatataga
     3961 tacgccagat caatcggctg ccaatgatca gtaatagcga aagtaggtat gcaaagatca
     4021 ctaaacgtaa actgtattgt ttatttatga aaagcaatat gatgatactt gaggccgcac
     4081 ttgcagataa cgaaccaaaa atcgcaatga tctctcttaa atttgtataa cgattgattc
     4141 gagtaaaaac tttgaataaa ctaccataga ataaataaaa accaatcgac aaaccgatag
     4201 agattaaaat aaggtccatt ggtatagcta caaacggctt catgaattta gctgctgcaa
     4261 tattcgcagc aatcaacaac aagctgtcca caaataccaa tgtggcaatt tttcttcttc
     4321 ttgttaatac aaacacccta cattccccct aaaataaacc aaacttcttc ttttttacct
     4381 cttcatagac tggataactg atctcatccc cgttgatcag gtcctttgcg acttgtttca
     4441 tctgtgctac tttttcctta ccaaagtcct tggcgatttg ctcataacat tcttttaagt
     4501 aaaaggtacg tttagagacc ccatgggcat cggaagcagc catttgcacg aggttatgtt
     4561 tgaccatcat tttggctgtt ttctggatgt cttttccaaa tacaccgaca taacttggtg
     4621 cggtgagttg agccaagcat cccatatcta ggaatggcag caaatgattt gggtcttctc
     4681 gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt tgtaacaact
     4741 caaaaaagag ccattctgca tataacggca cttccagtgt cggaaattcg attagaagat
     4801 atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttca atcagtgttc
     4861 cggtaatacg tacttcttgt ccttccaata acgtcaaagg caattgtctt ttctctaatt
     4921 ctgcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat
     4981 tattgtgatg gggtgtgcat aaaatatgtg tgatcccttg ttgaatcgct ttttcagcca
     5041 tggcgatact ggcatctatg ttttctgctc cgtcatcgat tcctggcagg atatggcaat
     5101 gtagatcgat catttctctt gtctcctctc tttttatttc ttaattcccg tagtaatagc
     5161 cagcatctct gctgtgttct gctccattgt atacaacacc aaggactcgc gcttgtacca
     5221 tatctaacag ttcttttgct ttagtcaatg attcttttcg tgacgtattt tcccgtacga
     5281 ccaataaggt gccatccgtt tttgaagaca tgatctgtgc atctgttacg gcaacgactg
     5341 gtggcatgtc gaaaatcact acatcaaata gttgtcttgc ttctgctaaa atctgatcca
     5401 ttcttggcga acttagtagt tctgatggat ttggtggttt aggtccactt ggcagaatcg
     5461 acaaattgtc gactggtgtt cgttggatct catctgctac acttcctgat gaactaagaa
     5521 cagtacttaa tccgcttgcg ttgttcaact tgaacgtttt atagatgacg ggcttacgta
     5581 agtctgcatc gactagcagt acccgttgac cagatttggc aaaaacaacg gcaatgtttg
     5641 ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc gttttgattt
     5701 gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga
     5761 ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttga gttgcttttt
     5821 gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg
     5881 ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gatccttttt tacggatacc
     5941 agcgttcaat tcttttgctg acatttctgg aactgttcct aagattggga atcctaatgt
     6001 atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata agaatgccag
     6061 acctacgcct accatcattc ctaacactaa gccaatcact agattcaatt tattatttgg
     6121 cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat cgacattcat
     6181 cacgtctttg gcgttttctt ggaaaacttg tgaagttgta ttagcgatct gttgagcagt
     6241 cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg actgattgac
     6301 ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg tttctaaacg
     6361 atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg tattgatcat
     6421 ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca gtgtcacgat
     6481 caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat
     6541 ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta atgtttcaaa
     6601 aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt tattctctag
     6661 attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcgc tgactttttg
     6721 ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct tgatcgtacc
     6781 aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca tgtctgtttt
     6841 catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttaaactcaa
     6901 tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac catagtcacc
     6961 atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtaat
     7021 tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat
     7081 accaccaact gcatcaacga gttcctttag ccctttcata ttgatagaga catagtgatc
     7141 gattgggatg tctacatatt tttgtagggt atccatcgcc atggcagctc cgccgaatgc
     7201 gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatctcg
     7261 aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt
     7321 atctgatctt ccttgatcga cccgacctaa atctccagta tcaatgccca tcaatagaac
     7381 agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc
     7441 cactgattcg taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac
     7501 tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa atattttttt
     7561 ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa
     7621 tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag caacagtttg
     7681 tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta tttgactgag
     7741 cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct tcaacatcgt
     7801 atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga atcctagata
     7861 ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt cacgtacacg
     7921 agcgacatct gcacttgtca ccttttcttt gatgacgaca tcgtctacag cttgctgcca
     7981 gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca gttttgattc
     8041 tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa cagcttcttt
     8101 aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacag aggatagctt
     8161 atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaat ttttttcttt
     8221 tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt tttgattggt
     8281 gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat tgatatggta
     8341 accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca atgacttgac
     8401 gaatggcttt tccatagatt cactcctaag tattgatgat agataaatta agatataatt
     8461 cacatacata aaaatgacag ctttttattt taaaaaacat aattaaatta atatattcac
     8521 agaaatattt
//
LOCUS       DAYYGY010000105         8282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000105 DAYYGY010000000
VERSION     DAYYGY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.107"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..382
                     /locus_tag="ACHA52_002591"
     CDS             <1..382
                     /locus_tag="ACHA52_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8941250.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="ACHA52_002592"
     CDS             complement(776..1783)
                     /locus_tag="ACHA52_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8941251.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="ACHA52_002593"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="ACHA52_002593"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8941252.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="ACHA52_002594"
     CDS             3679..4002
                     /locus_tag="ACHA52_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8941253.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="ACHA52_002595"
     CDS             4013..5695
                     /locus_tag="ACHA52_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8941254.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6865
                     /locus_tag="ACHA52_002596"
                     /pseudo
     CDS             5901..6865
                     /locus_tag="ACHA52_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6884..7111
                     /locus_tag="ACHA52_002597"
     CDS             6884..7111
                     /locus_tag="ACHA52_002597"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFM8941255.1"
                     /translation="MYQVFEEMGYPIKTVTRKLEVIKADDTISDLLDVEEGDPMFYFH
                     TQGFSLERLPVEYSISKYRGDINYFVFELAQ"
     gene            7326..8078
                     /locus_tag="ACHA52_002598"
     CDS             7326..8078
                     /locus_tag="ACHA52_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8941256.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
BASE COUNT         2688 a         1268 c         1686 g         2640 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga aggcgacacc attgtgggag ttaaacacaa
     6421 gagcgctgtt attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac
     6481 aggcagacga gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa
     6541 acacttgttt aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat
     6601 aagtaactcg aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg
     6661 gctaaatgag cacgagcact caaacggctt gcttagacga gatggcttac ctaaaacaat
     6721 ggactttagg gacatggatg aaacctttat tcaatccgtt gcctcgaaaa gaaaccatat
     6781 tccaagaaaa tcattaaatt atcgaacacc attggaagta tttttgagct gcatagataa
     6841 agacatattg tctagcttaa tttgacaaat cagaaaagaa aaaatgtatc aagtttttga
     6901 agaaatgggt tatccaatta aaacggtcac cagaaaacta gaagtaatca aagcggatga
     6961 tacgatcagc gatttgttag atgtggaaga aggagatcca atgttctatt tccatacaca
     7021 gggtttttcg ctagaacgtc tgcccgttga gtattcgatt tcgaaatatc gaggagacat
     7081 taactacttt gtttttgaac ttgcacaata acttcagatt aaataaaatg atttattaaa
     7141 gataaaagtc gaatgaacaa ggctgtcact atgacagtca tgctcgttcg gttttatttt
     7201 ttagaatagc agcttgcaaa ttctgaaaaa ttttgctttc cttcctgcag aaggcttctt
     7261 aagaaaactt gttctagcac ttttcttaag aatttgatag tcttaaaaag aaaagaggcg
     7321 atgaaatgtt aaaaacggat cgattccaag cgatcatgtc gttattagag gttaacggta
     7381 cagtacgagt cagcgatatt atggagaagt tttcggtttc ggatatgaca gtccgacgtg
     7441 atttggacga aatggagaaa caaggattat tagaacgcgt ccatggcgga gcgaaattga
     7501 aggattttta tcggcatgtg gagctttctc atagagaaaa acagattatg ctgcaagaag
     7561 aaaagcagcg gattgtggaa aaggcgaatg aaatgatcaa tgatggtgat acgatatttc
     7621 ttggaccagg aactacaatc gagctcatgt caaaaatcat tcaaggggat tcactgcgga
     7681 ttgttacaaa ctgtttgcct gtttttgaag aattaaataa gcgaaaaggg aatcgaaaaa
     7741 tctatctatt aggtggcgag atgcgggaaa cgactcaatg ctttgttggt gaaatcacga
     7801 ataaatcgct acaggatatg aattttcata aagcattttt cagctgtaat gctttaaatg
     7861 aacagaaaat tatgacttcg acttttgaag agggccaaac tcaacagatc gctctgaata
     7921 attctgttga acgcttttta ttgattgatc attcgaaaat tgggaaaaga gatttttcag
     7981 tatattataa tctgaatgat gtaacagtag ttataacgga tgatgatgaa caaaagcact
     8041 atgaaaaaat cgaaaaagaa gaaaaatatg ttgtgtaaat gagaaactgc caaactctag
     8101 ataattagaa cttggtagtt ttttggtagc cttgaattta agcgaattat gttattttta
     8161 gggtgcatca ttactaattc ctccgaatac ttgaatacac catttgaaaa aagatagtcg
     8221 atttttttgg gatgttatgt ttatgtgaag gaaaaaatag cagtatcaat caccgcttta
     8281 tt
//
LOCUS       DAYYGY010000106         8228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000106 DAYYGY010000000
VERSION     DAYYGY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.108"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACHA52_002599"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACHA52_002599"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8941257.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACHA52_002600"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACHA52_002600"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8941258.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACHA52_002601"
     CDS             2591..3376
                     /locus_tag="ACHA52_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8941259.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACHA52_002602"
     CDS             3389..4240
                     /locus_tag="ACHA52_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8941260.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACHA52_002603"
     CDS             complement(4364..4816)
                     /locus_tag="ACHA52_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941261.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACHA52_002604"
     CDS             complement(5281..5649)
                     /locus_tag="ACHA52_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8941262.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="ACHA52_002605"
     CDS             5799..6281
                     /locus_tag="ACHA52_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8941263.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="ACHA52_002606"
     CDS             6318..7328
                     /locus_tag="ACHA52_002606"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8941264.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="ACHA52_002607"
     CDS             complement(7443..8156)
                     /locus_tag="ACHA52_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941265.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DAYYGY010000107         7875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000107 DAYYGY010000000
VERSION     DAYYGY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.110"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACHA52_002608"
     CDS             <1..106
                     /locus_tag="ACHA52_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8941266.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(175..903)
                     /locus_tag="ACHA52_002609"
     CDS             complement(175..903)
                     /locus_tag="ACHA52_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8941267.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(916..2196)
                     /locus_tag="ACHA52_002610"
     CDS             complement(916..2196)
                     /locus_tag="ACHA52_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8941268.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2337..2939)
                     /locus_tag="ACHA52_002611"
     CDS             complement(2337..2939)
                     /locus_tag="ACHA52_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8941269.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3184..3390
                     /locus_tag="ACHA52_002612"
     CDS             3184..3390
                     /locus_tag="ACHA52_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941270.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3702..4421
                     /locus_tag="ACHA52_002613"
     CDS             3702..4421
                     /locus_tag="ACHA52_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8941271.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4502..5185)
                     /locus_tag="ACHA52_002614"
     CDS             complement(4502..5185)
                     /locus_tag="ACHA52_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8941272.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5404..7590
                     /locus_tag="ACHA52_002615"
     CDS             5404..7590
                     /locus_tag="ACHA52_002615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8941273.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2829 a         1317 c         1474 g         2255 t
ORIGIN      
        1 aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga
       61 tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa
      121 ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga
      181 aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac
      241 ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg
      301 acaacggata aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa
      361 ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata
      421 ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc
      481 tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt
      541 ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt
      601 taagattttc atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc
      661 taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat
      721 atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc
      781 cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt
      841 gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat
      901 catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc
      961 aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa
     1021 gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt
     1081 taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt
     1141 gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc
     1201 caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg
     1261 ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga
     1321 cagtaaacta cggatctcag gaactttcat attatccaac atacttagat tggccggaaa
     1381 aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc
     1441 taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt
     1501 gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct
     1561 gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc aataataaac
     1621 caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac
     1681 atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat
     1741 caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga
     1801 tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata
     1861 ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat cgccgatgct
     1921 tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg
     1981 ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc
     2041 agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac
     2101 tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta
     2161 aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt
     2221 accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt ttgaattcag
     2281 aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa
     2341 tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc
     2401 aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag
     2461 aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg
     2521 ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt
     2581 aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga
     2641 tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat
     2701 tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg
     2761 attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt
     2821 ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac catatcttct
     2881 tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc
     2941 tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca
     3001 taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa
     3061 gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt
     3121 gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt
     3181 cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta
     3241 tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt
     3301 gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt
     3361 gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag
     3421 gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca
     3481 tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa
     3541 aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg
     3601 gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa
     3661 atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca
     3721 ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt
     3781 tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt
     3841 ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg
     3901 caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata
     3961 gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt
     4021 atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac
     4081 cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa
     4141 gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac
     4201 tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg
     4261 atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa
     4321 tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta
     4381 caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt
     4441 gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag
     4501 atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg
     4561 ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac
     4621 agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa
     4681 atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc
     4741 cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag
     4801 ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat
     4861 ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc
     4921 gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc
     4981 ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa
     5041 atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt
     5101 ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac
     5161 gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc
     5221 cttatcttag cataaacgat tgaaaaaaaa ggaaaaattt tcagaaaact aaaatcatat
     5281 cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata gacaacgaaa
     5341 ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat
     5401 cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg ggagaacaag
     5461 tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac ctatgaagcg
     5521 gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga aagtgaagga
     5581 aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa tcaagaacaa
     5641 aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg agctgggagc
     5701 ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa acaagtagac
     5761 gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact aaaaaaacga
     5821 gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca tagtatcttt
     5881 tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa
     5941 ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt
     6001 aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc tcggttagac
     6061 tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa agaaaaatat
     6121 gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg attgctcacg
     6181 gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt actccaaaat
     6241 ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa atatgattat
     6301 ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt gaaacggatg
     6361 acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag tatctatgga
     6421 ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc taccaaaact
     6481 cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc aaatagattg
     6541 atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca aaaagaagca
     6601 ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg gctaatcaaa
     6661 caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat attataccga
     6721 tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat tccttttgta
     6781 ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca acatttctat
     6841 caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca ggcctaccag
     6901 caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac
     6961 ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat aagccccaat
     7021 ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa aacagaccag
     7081 catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat agctaaggaa
     7141 atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc tactttcaga
     7201 agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa aacccgcttg
     7261 attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca caaaagtaaa
     7321 ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt gccttataac
     7381 ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc agtaacgacg
     7441 atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa tcgtctgtat
     7501 ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt tatcaaagaa
     7561 accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag aagtggtcag
     7621 tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaatttcagc
     7681 ttattttcga agaagctatt tctggaacac agtttattcg gaaaagggag gtgtgacaag
     7741 aattttgtca cacctccctt atttattttg aaaatgagaa tactattatc aaaatagaga
     7801 atatatagaa aaattctcat tattcaaacg taaataatca gttattttga ttttttttat
     7861 attttattag ctaat
//
LOCUS       DAYYGY010000108         7826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000108 DAYYGY010000000
VERSION     DAYYGY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.111"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1139
                     /locus_tag="ACHA52_002616"
     CDS             201..1139
                     /locus_tag="ACHA52_002616"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM8941274.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1391..2401
                     /locus_tag="ACHA52_002617"
     CDS             1391..2401
                     /locus_tag="ACHA52_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFM8941275.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            2769..4193
                     /locus_tag="ACHA52_002618"
     CDS             2769..4193
                     /locus_tag="ACHA52_002618"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8941276.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            4279..4707
                     /locus_tag="ACHA52_002619"
     CDS             4279..4707
                     /locus_tag="ACHA52_002619"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFM8941277.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            4795..5961
                     /locus_tag="ACHA52_002620"
     CDS             4795..5961
                     /locus_tag="ACHA52_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8941278.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            7177..7296
                     /locus_tag="ACHA52_002621"
                     /pseudo
     CDS             7177..7296
                     /locus_tag="ACHA52_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(7344..7577)
                     /locus_tag="ACHA52_002622"
                     /pseudo
     CDS             complement(7344..7577)
                     /locus_tag="ACHA52_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2673 a         1024 c         1450 g         2679 t
ORIGIN      
        1 agagaacaaa agtaaagaat tatatctact tgcttgcgta tttctttttt atcattgtgt
       61 gtttgatatt catcaatata agaggaactg cttttgtaaa tggaatccta ccattattta
      121 gagaaaatat tatttttaag gctgtttact atttttttga gtaagaataa atagcactta
      181 aaaatagaga tgagggattt atgggaagac aagaattata tagtttagct atcaatgaat
      241 tggaaaaaag aaatcttgaa atacatgaat taaatcaaaa aaataattct attttaggca
      301 tgaaaatacg aaataaaatt aatcaactta accaaatgaa aaaacgagat aatctgttgc
      361 cagcaaaata tacccttaaa tcgatgaccg gtaaggggcc tattaaagac tattcaaaca
      421 gagagaatcg cagtgatgaa ctaagaaaaa aagagttaac taatcaagga acacctaaaa
      481 ggattgctgt gtatagttgt atcactggta attatgattc tattaaagaa ccggattttt
      541 ctcatccatt ggtagattac tatttgtata cagataatgt gattacaagt gagcattgga
      601 atattagaga gatacctaaa tatatatcgg aattaaaaaa taatattttg atcaatagat
      661 atattaaatt tcatcctaat gagctattta ctgactacga ttttactttg tatgttgatg
      721 gtaatgtaac aataacttct gatattaccg gattactgaa cagttcttcc aaacttgggt
      781 taagtatgca taaacatagt gaaagaaaat ctttatattc tgaggcagat gcattaattg
      841 cagttggcaa aggtaataag gaaagtatta tagagcaaat tagtaagtac agagaagaag
      901 gatttcctga taattttgga ttggctgaag ccacggttat tttagttgat ttaaataatt
      961 caatcgctga gaatcttcta gatttgtggt ggagagagta tttgaactca gaaagtgggc
     1021 gagatcagct cgcatttcct tacgtgttgt ggaagtccgg acatgcaatg aaggaagtga
     1081 ctaccttagg caataatgta tataaaagcg ctaaattaag aatcataagt cattattagc
     1141 cctaaatgat ttctaaacta ttctttcaaa tcctagtaaa tatttggaga tagaagattt
     1201 cctttttaaa gtcataaaaa attaagttat attttttgag tgaatggaga agacgcatag
     1261 tatcataaac ttcgttatat cttaaattat ggggtatgcc taaataataa atgtaatacg
     1321 agtataaaga agaaagcaat tttgggttta accctattgg ttcctttcag aaaaaggtgg
     1381 atgaataaaa atgaaaatat taataacagg tggagcagga tttattggtt ttcatttgtc
     1441 tagaaaatta ctcaaggaaa ctgaaaatca aattgtagcg attgataatc tcaatgatta
     1501 ttatgatgtc caattaaaag aagatcgatt aacgatactg tacgattata ataattttaa
     1561 atttatcaga atggatctat tagagaaaga aaaattagat tctctatttg aaagtgaagg
     1621 ttttgacata gtaattcatt tagctgctca agcaggtgtt agatattcta ttgaaaaccc
     1681 ttatgcttat attgattcta atattatcgg ttttcttaat attcttgaag cctgccgaaa
     1741 cttccctgta aagcatctat tatatgcatc atctagttca gtatatggtg ggaacacgaa
     1801 agtaccgttt tcaacagaag attcggtgga tcatccagtt agcctttatg cggctaccaa
     1861 aaaatcgaat gaattaatgg ctcatactta tagccacctt tataaaatac caacaactgg
     1921 gctgagattt ttcactgtct atggtccatt tgggcgacca gatatggcct atttctcttt
     1981 taccaagaat attttatctg gaaaagaaat taagattttt aaccatggaa agatggaaag
     2041 agatttcact tacatcgatg atatcgtaga gggaatcact agattaatca ataaagtacc
     2101 tgaacccaac tccgactgga cagacatcga tgatttgagt agtgaaagtt gggcaccata
     2161 taagatatac aatattggaa ataataagcc ggttgcattg ttggagtttg ttgaaacaat
     2221 tgaaaagtat ttaggtgtta aagcgaacaa gatttttttg gacatgcagc cgggtgatgt
     2281 cgttagaact tatgccgata ttgaagattt agaggaaaaa atcaacttta aaccgaatat
     2341 aaccttggat gagggcatta aaaaattcgt tgaatggtat acagtgtatt atttgagtta
     2401 attttgaaag gaatattgaa gatgggtaat tcatacgatt tttttagctt tgttaggtat
     2461 tttaggaaat tctactttca aacgtgacga actttctcgc cgaatagata ttttctataa
     2521 ctaaaagaat ttctataaaa caaaattgtg tatcaagatg tgtgaactgg attctgactg
     2581 ccaatattga ttgatgagat tgaagaaata tatagttgat catggatatc tttatttgaa
     2641 aagatcttag ctattcacat attcaaaaaa atttttacta cgaggactca cacgatacag
     2701 aggaatttag gtagttctaa ttaaaccaga aagtcatcta tagatgtgag ttgagggagg
     2761 tcatacggtt ggatagtagc agtaataaat cacattcaat agctaaaaaa gcttttattt
     2821 acgcaatagg aacattatca tcaaaaatga tatctgtcat tataatccct atctatgcag
     2881 tatatatttc tgttgccgat ttaggaagct ttgattatca aaatacaatt gctcaatttc
     2941 tttctccagt tatttttgga gcaatatggg aagcaatttt acgcatggga ttaggcaaag
     3001 aaaaaaatat tcagaaaaaa gtagtgacga acagtatttg ttttgttata gcaaatacag
     3061 tgatattagc cattttttta ccaataatat atagaatgat tttcccgaat ttagagtatt
     3121 atcaattgtt tgtttttcaa ttaattttca ctcctatcct aagtgttttt caatatattg
     3181 taagagcact aggtgaatca aagatttttg tgatgagtgg tataatcgct gcgggtatca
     3241 acctgttact ccttactatt tttgttgtta caatgggtat ggggatactt ggtctcttac
     3301 tctctatcat aatttctaat gttataaatt tgattttttt gattttcagg gggcagatct
     3361 ttcaatattt cgcaacgagt tcttttgatt ttttattttt aaagtccatg ctgaaatact
     3421 ctatcccact cactttgaat ctcacttttg gatggctaat agttggtttt tcaagatttt
     3481 atattggaac gtacatcgga gtaacagaaa atggtatttt ttcttttgcg atgaaatttt
     3541 catctataat tttaactctt acaagtattg ttagtatgtc tatgatagag gatgcggtta
     3601 tcacgtctga gaatgatgat ttcatcgaaa gattcgttga gaaaaatacc tatattttta
     3661 aaatgtacat taggttagcg actctttcta ttcctatcat ttgctgtgca tactctttta
     3721 taaccaaccc tgattataaa aaatcattag ttctagttcc aatattagtt attatggcat
     3781 gtttcagtgg gatgacaaca aatatcggaa atatatttgc agtatacaat aaaacaaatc
     3841 agttattaat gatttcagta gtgtcaggtc tattgagtgt tgttggcacc tatttattag
     3901 gctattcttt tggagtaatt ggtgtctcat ttgctacttt gctcggtact ttagtattat
     3961 ttggacttag atattatttt ggaaggaaaa ttgcttatta tgcatttgat tggaagcgaa
     4021 tatcgctact tttagttctc tattccgcaa tcgcttttgt atgcattcat agaggcttct
     4081 attttaatct tttcttaacg attattttta gtgctatttt cgtttttacg tataaacaat
     4141 taattataac gtatctaaaa aaatttttca gaataaaaat ggctaaaagg tgagggtata
     4201 cgaagtaaga atagaatctt tcgaagaata aagagaaaac tatttggaaa aaatctgcga
     4261 gaatgtatta atgaaggttt gatattcgga aaaaaacata gtttagtaga ggttcctgaa
     4321 tttgggagcg agccatattt aattgaaata ggggataatt tcagatgttc atttggtgtt
     4381 aaatttataa cacacgatgg tggagtctgg gtcttaagga agatgagttc tgtgagcgag
     4441 tcaacttcaa aaataggtag gatcaaattg ggaaataata tttttattgg tatttactct
     4501 attgttttcc caggagtttc aattggcgat aatgtaatta ttagagcgaa cactttagta
     4561 aataaagata tcccaaataa tagtgttgta gcgggtagtt catgtaaagt gatttcaact
     4621 atagaggagc attatgagaa aaataaaaat gaatttatta aatatcgaac ctcgatgatt
     4681 ttgaagcaaa aaaatttttt ctattaaata atattgattg aataaggaat atgttcaaaa
     4741 ggataacagc taaaatacta taaaatgaaa tttgattgga gcgtatggaa gaatatgaaa
     4801 atttcagttt tcggattagg ctatgttggt ctagcaaatt cactattatt agcacaaaaa
     4861 gagcaggtca aagcttatga tatcgtggaa gaaaaaatca cgatgttaca aaatggcatc
     4921 tcaccaatag aagataaaga agttcatgaa tttttaaaac gggatgactt aaatgtcgaa
     4981 tttacgtcaa attttactga tgcagtaaac ttcggtgatt acttgattat tgcaactcca
     5041 acagattatg acgaaaagaa aaactatttt aatacatcaa cagtagaaga tgtaattgag
     5101 aaggctttag caattcgtcc agattcaaca ttcattatta agtcgacagt tccagttggc
     5161 tatgcaaatg aaattaaagc gaaatttggt acagaaaaca tcattttttc acctgaattt
     5221 ttgcgtgaag gaaaagcttt gtacgacaac ttatatccat caagaattat agttggagaa
     5281 tgttctgctc gtggtcgcga gattgcaact atgtttcaaa gaaacgcatt taacaaagat
     5341 gcatcagttc ttttgactca ctcaactgaa gcagaagcta ttaaattatt ctcgaataca
     5401 tatttggcat tgcgagtggc gtatttcaat gaattagata cgtatgcaga atttcgtggg
     5461 cttgatagta agcaaattat tgaaggtgtc ggtttggatc cacggatcgg ttcgcattat
     5521 aataatcctt cttttggcta tggtggatac tgtttaccca aagacacaaa acaattaaga
     5581 gccaactatg aaaaagtacc aagcaatatc atcagtgcaa ttgttgatgc aaatacaaca
     5641 aggaaagatt ttattgctgg acagattttg gcgaaaaaac caaatgttgt tggaatctat
     5701 cgtttaacga tgaaggccgg ttcagataat tttcgatatt cgtcgatcca aggaattatg
     5761 aaacggatta aagccaaagg aattgaagtg gttgtttacg agcctacatt ggatgaagat
     5821 gaattttatc attcacaagt attgaaagat attaatgaat ttaaaaatat ttctgatgtg
     5881 atcgtatcta atcggtttga agatatttta aaggatgttg aagaaaaagt ttataccaga
     5941 gatatctttg gtgataacta aataataatg aacgggtaca tggaggaaaa tattttagat
     6001 ggtgactcga aataaatgtt tgttctcatt gcatctgtat ctataccgat aagtttaaaa
     6061 attccgatta ggaaaatgaa gttggctatc cttgcgagga atttattgct ggattatttt
     6121 atgaactaaa gcaagacatc atcagaaaaa aatccaaaat ttgacaatgg gattgatcgt
     6181 ttaccagctg taagcgaagt atctgacacg aaaatggaaa atgcaattac tgttattacc
     6241 aatagctttt tttcatcgat caagtatttg aaaacattat ttgtttgcca agctgatcaa
     6301 tgttctttca gacttggaag aacattgatc agctcacaag taatgattga agtttaggtt
     6361 atgggataaa ggataagtga aataaaagtc gtgtctgcat ctttgttagt accagtaaat
     6421 ttttctacta cggcgctagt cacgacagtc caggcatctg aaattgaaga tgcaaataga
     6481 gtttctgcta gtttaggtat tgatgttact gtattagcat taatcttaga gccagtagca
     6541 tccaataggt gtagctatag ttctaatagt ataaaacaat gcagaggata tgattgcttt
     6601 atgtctcggt gtgtcaggtc aaaatagagt tccatatggg gcgatttctc aatgaataat
     6661 cttatagata taatcttgtt tgctatttcg ttatcacttt tacttttctt cgattatttc
     6721 aaaaataaaa atccaaaata ttttaagttc ttactattta tcattactgt actagctgtt
     6781 ttatcgatga cagattttat ttaacttatc tcaaaaagta gggaaccata tcatttttca
     6841 gtaacatgcg tattatttgt ataaataata caaaatgaaa aattatgatt attctattgt
     6901 ctttttagaa agcgttttct gtgagctttt aatctaaaag gagggatata aaataaagaa
     6961 aacctatttt atgctatttt tttgttgttt ctgtgtagga atcctgtggt atataggtgg
     7021 taatgaaaag aagattcatg gagttgtgac aaatatttct gcggatgagt tcgtatttga
     7081 agatgaaaaa cagaacgaat atagtattca aaaaagaagc tctttgtcaa atgatgttgg
     7141 taaagagaaa tcttagaata atttggacag aagaggctgg gacagaagtg tttaactccg
     7201 agaaataaga agaaattcac gaaaattgct tttcaaattt ttgtgaattt cggcttattt
     7261 ccgaaggagt tgcttctgct cccgccgttt actcgagagt ctgtagcaaa actgattttt
     7321 agatttatcc cacattcttc tgtttatttg gttaaaaatg ttttgccatg acgaatcatg
     7381 atccgtagtt tgaaattgta aaagctgcga aagccatacg tattgcgttt taatactttg
     7441 atgtggttgt ttaaacattc caatggtctg tttgaatagc tgcagttaag cgcattttga
     7501 atctgtggca gaaaattttt aaacgttcgt aaagtggttg tataggtttc aggcagatcg
     7561 ctaatgtctc tagttaaaca gtcctcaaat agtgacgaat ctcttttctt tcactcaatg
     7621 attcaagaag gttcttttaa tatattggaa gatgtggata ttcttctggt gtgtactgaa
     7681 gagtcccttt gtaaatcaag caaatttgtc ctttgtattt ttctttagtt aaacaatttt
     7741 cctcgaaatg gatattgata tttaggtcta gaatatttag ggtatctttg ataagcagag
     7801 tatgagacat cttgaatcct cctatg
//
LOCUS       DAYYGY010000109         7601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000109 DAYYGY010000000
VERSION     DAYYGY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.112"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHA52_002623"
     CDS             complement(138..428)
                     /locus_tag="ACHA52_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941279.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHA52_002624"
     CDS             complement(546..806)
                     /locus_tag="ACHA52_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941280.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHA52_002625"
     CDS             complement(943..1356)
                     /locus_tag="ACHA52_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8941281.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHA52_002626"
     CDS             complement(1812..2297)
                     /locus_tag="ACHA52_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941282.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHA52_002627"
     CDS             complement(2436..2651)
                     /locus_tag="ACHA52_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8941283.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHA52_002628"
     CDS             2875..3675
                     /locus_tag="ACHA52_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8941284.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHA52_002629"
     CDS             3694..5562
                     /locus_tag="ACHA52_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8941285.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHA52_002630"
     CDS             5555..6046
                     /locus_tag="ACHA52_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8941286.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHA52_002631"
     CDS             6034..6588
                     /locus_tag="ACHA52_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8941287.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="ACHA52_002632"
     CDS             6606..7601
                     /locus_tag="ACHA52_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8941288.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAYYGY010000110         7468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000110 DAYYGY010000000
VERSION     DAYYGY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.113"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..1203
                     /locus_tag="ACHA52_002633"
     CDS             25..1203
                     /locus_tag="ACHA52_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8941289.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
     gene            1591..3537
                     /locus_tag="ACHA52_002634"
     CDS             1591..3537
                     /locus_tag="ACHA52_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8941290.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            3540..3995
                     /locus_tag="ACHA52_002635"
     CDS             3540..3995
                     /locus_tag="ACHA52_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8941291.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            4009..5337
                     /locus_tag="ACHA52_002636"
     CDS             4009..5337
                     /locus_tag="ACHA52_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8941292.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            5371..5655
                     /locus_tag="ACHA52_002637"
     CDS             5371..5655
                     /locus_tag="ACHA52_002637"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8941293.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            5657..6172
                     /locus_tag="ACHA52_002638"
     CDS             5657..6172
                     /locus_tag="ACHA52_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFM8941294.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            6188..6850
                     /gene="rpe"
                     /locus_tag="ACHA52_002639"
     CDS             6188..6850
                     /gene="rpe"
                     /locus_tag="ACHA52_002639"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8941295.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            6857..7429
                     /gene="hxlB"
                     /locus_tag="ACHA52_002640"
     CDS             6857..7429
                     /gene="hxlB"
                     /locus_tag="ACHA52_002640"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFM8941296.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
BASE COUNT         2566 a         1105 c         1405 g         2392 t
ORIGIN      
        1 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
       61 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      121 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      181 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      241 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
      301 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
      361 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
      421 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
      481 ttaaatgata agtatgtcgc tatttttatg gacgctactt acattccact aaaacgtcaa
      541 accgtatcca aagaagcgat ttatattgcc attggtatac gagaagacgg cactaaagaa
      601 gtactgagtt atgcgattgc tccaactgaa tcaacatacg tttggaatga gctgctacag
      661 gatattaact ccagaggagt tcaagaagtc ttgcttttta ttacggacgg cttaaaaggc
      721 atgaaagata ctatccatca aatttatcct aaagcaaaat atcagcattg ttgtatccat
      781 gtatctcgta acatcgctca taaagtacgt gtcaaagacc gaaaagaaat ctgtgatgac
      841 tttaaggctg tttatcaagc taactcaaaa gaagaagcga ataccttctt atccggcatg
      901 attgagaaat ggaagaaaaa ctatcctaaa gtgacgcagt cactcataga aaaccaagac
      961 ttattaactt tttatgattt tccacctagc attcgtagaa ccatttactc aaccaatcta
     1021 atcgagtctt tcaataagca aattaaaaga tacagccgta gaaaagagca gtttcaaaat
     1081 gaagaatcac tagaacgctt tctagtcagc atttttgata catacaatca aaaatttcta
     1141 aacagaagcc ataaaggttt tcaacaggta accgatacat tagtttcaat gtttactgag
     1201 taactaatta ttttgcagga ggacaattta tttacacaaa attattgacg ctcccaatta
     1261 ggactcaatt gttcatagat gagcatttgt aattaagtta tattataata aaaaaatacg
     1321 aattttagtg acgccattat attgttttta tttttcaatt gtaatatttc atattacttc
     1381 cataataagg agtattttct attggaatat cttagctctt ttaaggattt ctcctttttt
     1441 gtttctcatt ttttaagatt atttggattt cctttacaac aaacttccac aagtattatt
     1501 gacatagatg taattgaggg aagggggttc cagtgctaga ataaataatg aaaagaggta
     1561 acaaatgcta gcgttaagag gtgaacaagt ttgattgaaa gacatgtaaa tttattaaaa
     1621 ttattaagtc gacaagaaga ctttaaaccc gcttcttttt ttagtgataa attggcagtg
     1681 tcgaccaaga ccatttatca agatattgaa ttgttggaag attatgtagt taattaccaa
     1741 gtaacaattg agcgattacc ttctttgggt atcaaactca taggtgatag gaaaaacgtt
     1801 gaacatttga tttcatcggt agaaaattat cttgaaaaag atatttattc tccagaaaaa
     1861 agacgtgtcg agatcattaa agaagttttg tttggtgata agcctgtttc tttaactcat
     1921 ttgtctcaaa gttttatggt tagtaaaaca tcgttataca atgacctgag aattatcaat
     1981 aaaattattg gcagtggaga agttaagttg cagtcatctg cagaaggaat tagttttact
     2041 ggtaatgaac aagcaattca acgagcaatc aagcaacttg tattctatta tgcgaatgac
     2101 atggaatcaa aaacattttt tcaagtttta aatatgttgt ttgattcaaa agttattagt
     2161 gtcattcatg atttgctatt caatgattac agtgaactga ctgaaaaggt ttctgattac
     2221 tatgttcggt cattgatttc tactttagtt atccaatgta accgtttact gaaaggtcat
     2281 catttaactg ttgaagaaga attcctcttc aatagcattc gctatatgga aacgtacatt
     2341 gtggcaaatg gtttaattga agaattgaag gcaaaattga atcttccatt ttatgacgca
     2401 gataaagaat atctatgtcg tcaactattt gcacatcgtg tgacaaataa tttaaaaacc
     2461 agcaatgaag agtacgctat gagcgttcat aaattgattg aaagaatgag tgagattgaa
     2521 aaaattgatt tgactcaaaa tgatcgatta tatcaatcct tattgtatca tgtgccagca
     2581 atggtccttc gtttaaaaaa aggtattaaa attcaaaatc cgttattaga aaatatcaaa
     2641 tctcaatatt cagaactctt tagcatagta tggtacgcca tgtccatcct tgaagaagaa
     2701 tttgatgtga ctttaacgga cgatgaagtt tccttaattt tgattcattt tcagattgct
     2761 attgaagatc agtccaaagc aaataatatc atcattattt gccaatacgg tatttcttct
     2821 gcgcaattaa tttacaataa agttcgacgg tttttaccag caaaagataa tgtagaaata
     2881 tcaactttag ataagtttca tttagctgaa attggggaag ttgatttgat tatttcctct
     2941 ctttcctttg atgcaggtgg tataccctta gtaagggtat ctccattggt aagtaaaaca
     3001 gattatatga atattatgga agcttatact aaatatttaa tagaagaacc ttcgcgattt
     3061 attcaaaatg aactgattcc taaagcggtt aaagttccca ccatatctaa atttgttcaa
     3121 tcatctttag taaaaacagg agtacaggtg gaaagcaaag aagaatgttt ggatttgatg
     3181 atttccgaac tagaagccaa ttcattagtt actgaagagt ttcgtgcttc tatcttctct
     3241 agagagaaaa tggggaatac taatttagaa agtggtgttg ctttaccgca tgcgaatcca
     3301 tcaatggtca ttgaatccaa catttttatt gtagcattaa aaaatgcgat taattggggc
     3361 gatcgaaaag tcaaattagt cattatggtt agtttagctg aagaagatgt atctgagatt
     3421 agaggagtat atgaagaact ttaccaattt attgggcaaa aagaaatggt cgatcgcttg
     3481 ttacaagttc atgatagcga aaaattacta agaatttttc aacaaaaagg agaataaaaa
     3541 tgtatctaga taaagaatta tgtttatttc aattagaagt gaaagatcaa aaagaattgt
     3601 ttcaagtaat gtctgaacaa ttaaaaaacg ctggttgcgt gaaagatagt ttcttagaag
     3661 gtatcaccaa ccgtgaacaa gaatttccaa ctggattaga agtaaatcaa attgggtttg
     3721 ctatcccaca tacagattcc gcacatgtaa atagctctca aatctgtttt gcctcattaa
     3781 aggaaccact ggtattttca gatatgacag ataaatctca tgaaatacct gtacgtttag
     3841 tctttatgtt ggcaatgtca caacctcatg aacaaattga tacactacaa aacttagtta
     3901 gcctatttca aaatgaagaa aaagtaaatg aattacttgc atgtaccacg aaagaagcat
     3961 ttattgagtt agtacattct gcaggagttt attaggagga atagagagat ggatattgta
     4021 ttaaatgcct ttgaatggtt tattggatta gggtcaaacg tctttttacc actgattatt
     4081 tttgtaatcg ggatattatt tggattaaaa cctggtaaag catttgtttc cggcgttact
     4141 gttggtatcg gttctattgg tttaggatta gttttaagtt tattatcaga tggtttagga
     4201 acagcaattc aacaaatggg ggaacaatac ggtggtggcc tgaatatcct agatattggc
     4261 gtaggtgttg gtggaccttt ggcttactct acatcattgg gaattttaat gattccaatt
     4321 tcattaattc ttaacttcat atttgtggtt ttgggttgga caaaaacatt gaatgttgat
     4381 atttggaact tttggttcgc aattttccta ggtatgatta ctcaatctgt tacgggaaac
     4441 ttttggtttg gcttaattgg cgcgattgtc gcagtaattt tacaatggtt cttagcagat
     4501 cgtacacaaa aaactgttag cgaatttttc ggctatccag gaatctctat cacacatatg
     4561 atgggattat caggagtact atttgccaaa ccagtaaatt gggtttttga tcgtattcct
     4621 ggatttaata aaatagatgc agatgcagaa acattaacaa aacgtttcgg tgttttaggt
     4681 gacacagttg ttattggcct aattatcggt attgtagtgg gtattatggc aggctatgac
     4741 gtagcaggaa ttggaaactt aggaattatg actgcggcag tgatgaagat tatgcctaaa
     4801 atggtagcaa tgtttatgga aggtttaatg ccaattgcag aagcggcgaa agaatttacg
     4861 aataaaaagt taaatggtcg ccaagtaaac attggaatgg atgcagcgtt aactgtgggt
     4921 catccaacta ttatgtcaac ttctctattg cttgtaccaa tctcattatt gctagcacta
     4981 attcttccag gaaataaagt attacctttt ggagatttag cattctttgc tttcgccatc
     5041 tgtttaatga ttccttattt taaaggaaat attgtgagat cattagtagg atgttcgttg
     5101 tatgttgtta caacattata tatggcaaca tggttggcac caattatcac tgatgtgttt
     5161 acattagcaa gtttcgatgt ggggacagca ggattagtaa ctatgttgtt atgtggatta
     5221 tggccagtag ggttgtttgt tttattagca tcttactttg gtgtaattgg aataggtgga
     5281 ttaggagttg ttttagtagg tgcttctatc tacatgaaca aagtgaagaa agcttaatca
     5341 taaaattaaa ataagaaaga agagatcaat atgaaaaaat tattagttat ttgtggagca
     5401 ggtcatgcta cttctacaat tgcagtagca aaattatcaa aatggttgga agaagaagga
     5461 ttaacagatc aagtgaaaat ttatcaatct aaaattgctg atgagctaaa taaaattgat
     5521 gaatatgatg ctgtagtaag tacaacaatt gtccctgatg taattaaagc taaagtaacg
     5581 aatggcttgg gcttattaac aggtattggt acagatgcca tttatgcaga tgtaaaagaa
     5641 agattaggtg tttaatatgc gttttcatca tgacactttg actattgaaa cagtttcagg
     5701 gcgtccttcg tatcatgata ttcgaaaaga acttttagat acagttgata cagcaggtat
     5761 tgagaatgga atgttagtaa tttcgagccc gcatacaact tgctcactct tttttgaaga
     5821 aacgatgcat gacacaaact tttttggtga tgattattta catgtagaca tcaacaatgt
     5881 aatggaaacg attattccaa aaatgacgag tgaaaatcaa tacaacagtc cggggccaaa
     5941 acacattgat tttggaacaa gattatctga tcccaattac cctgcagaaa aatgggtcat
     6001 gctaaatacc gatgcacatt tacgttcatc tatttatgga accaactcaa tgactttaat
     6061 tgttcaagaa ggtaaacttt tattaggaga aatgggccgt gtctattttg tcgattggga
     6121 tcaactaaga gagcgaacta gaacaatcaa tctcatgatt atgggagaat aaacggggga
     6181 atagagaatg acaattgctc catcattgtt aaatgcacct atcgcagaag ttaaagagtt
     6241 atttgaaatc ttaaaagaaa agaatgttga ttatttgcat attgatgtaa tggatggctg
     6301 ttttgtacca gatatggctt ttggaccaag tgttattaaa gatattcgtc cactaacaga
     6361 cgttgtgttt gatgttcatt tgatgattga tacaccagaa aaaattgtca caagatttgt
     6421 agaagcagga gcagatattg ttaccttcca cattgaagct accaaacacg caatgaaggt
     6481 cattcagtta gtcaaggaag caggagcaaa agtagggatt acttttaatc ctgcaacacc
     6541 agtcagtttt attgaaccag tattatcttt agtcgatcaa gtattagtaa tgacaatcaa
     6601 tccaggaaca gagaataagc attttattca agagacagta gttaaaatcg aacagttaga
     6661 tgtaatacgc aaacaaaatg attatacgta tgatattgaa gtagatggca agattgataa
     6721 tcaaacgatt aaagtttgtt ctaaagcagg ggcggatatt tttgtatcag gcggtttcat
     6781 cttcaatggg aaaaataccc cgcaagaaca aattgataaa ctaaatcaat cattagagga
     6841 agtgaagtaa aagatcatga ccaataaaga ccaaaataaa aactatttag aagcgattat
     6901 cttagaatta caaaataact caaaacgtat taattctgta gagttagaac aatttagtga
     6961 gaaaattgtt caagctaaac acatctttct agctggtgca ggacgctctg gaattgccat
     7021 gcaagcattt acgaatcgct taatgcattt aggtttttca gtcagccttg tcggtgaaat
     7081 tagttcacca cattcgcaac ctggagattt attaattatc tgttctggct caggtgaaac
     7141 aggtagttta aaaaatttag cggccaaagc aaatcaaagt ggtgtaagca ttgctttagt
     7201 taccatgaaa aaagactcgt cgattggtca attagcagat tctgttcttg tattaccagg
     7261 tattaccaaa gatgaaaatc aacgagaaga agatactttt tcacaaccta tgggttcaag
     7321 ttttgaacaa ttagcatttc tcacctttga tagtttagtg ttatctttaa tggataaaac
     7381 aggtgaaagc tcagaaacga tgtttgctcg acatgcagat tttgagtagt agattttaaa
     7441 gataaaaaac cattctctaa atgctaat
//
LOCUS       DAYYGY010000111         7296 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000111 DAYYGY010000000
VERSION     DAYYGY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.114"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..909
                     /locus_tag="ACHA52_002641"
     CDS             <1..909
                     /locus_tag="ACHA52_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941297.1"
                     /translation="AKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQKSSER
                     FMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFANSFWF
                     LKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLK
                     RIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLF
                     DLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLD
                     FIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            1092..3767
                     /locus_tag="ACHA52_002642"
     CDS             1092..3767
                     /locus_tag="ACHA52_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8941298.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3949..4188
                     /locus_tag="ACHA52_002643"
     CDS             3949..4188
                     /locus_tag="ACHA52_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941299.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4204..5577
                     /locus_tag="ACHA52_002644"
     CDS             4204..5577
                     /locus_tag="ACHA52_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8941300.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5581..7215
                     /locus_tag="ACHA52_002645"
     CDS             5581..7215
                     /locus_tag="ACHA52_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8941301.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
BASE COUNT         2601 a         1136 c         1442 g         2117 t
ORIGIN      
        1 gcaaaaaaga taaattttga tgtggataaa ctttataaaa gtttgatagt tgttcccaaa
       61 aataaatcag aagatgggaa ttatgagtta gaagttttgc aatttaagat tgaacaaaag
      121 tccagtgaaa gatttatgag caatcaatta gtgaaaatca aaaaatggct ccctatgatt
      181 gatcaattag tatctaataa taaggaagct aaaaatttat gtatttttgt cttattatta
      241 catggaagag ttattaactt agttaatcga aaaagtacga ctaaatttgc aaactcattc
      301 tggtttttaa aaaatattaa taaaacagtg gaaaagaatg gagtttataa aggtgatttt
      361 cattttcaaa atgctgagac tattgaacga gaacatcatt atttcgattt attagtgaat
      421 acagtggtag aattagcgaa atgggcaaag tcaaacgaaa cttatagtca agtacttaaa
      481 agaattatga gtcaagaaac ttcatttatc tatgagttaa gcaagtcaaa tttgaaatat
      541 acttataatg aagatgaact tgaaatttta gataatccag aaaattcaca atggattaaa
      601 gagttgaaga gagaagactt tattgagaca ctaagaattc taaaatcgtt atttgatttg
      661 aaattagcca aagctaccat catgggaatt gaaagagatt catattattg ggaggaaatt
      721 ggactatatg ctccccagat tgaaggtgaa gataaagaga aaaggataga caacattatt
      781 aactatatta atagtttaag tgaaccttta aaatgtagag gacttgattt tataggaaca
      841 ttaggatgga cagaaacata ctatgagaaa atatataaag aaataattga acaatatttt
      901 cctaaataat ttaataagga ttgatttatc gtaaaagaaa aaagttgtga tttccaaaat
      961 cacaactttt ttcttttgtc aagattttga gtgggatata tttttgtaga actgaagatc
     1021 atgaaattat gagaggttgg aggtaatgta gtcatacaaa taaaaatttt ttagaaagtg
     1081 agtggtaatt aatgaaaatg aatcacactt caattcagaa gcatttacaa ctgttaaata
     1141 cactggggat ttcttatgtt aatttgagaa ctttttctac attaaacaag aaaaccgttt
     1201 ctcatatttt ttctactgca gattctcaag caattattaa ctatttagaa aaacagaaat
     1261 tgtctgaagg tacagtaaac ataacttata atcctattaa acaagaggtg attacacaaa
     1321 aaaagcattc tgtgagagac aatgatattg aaaaaattcg gtttgtattt attgatattg
     1381 accctaaaag gaaagaggga agtgcaacag atagcgaaaa gaaaaaggca gaaaatgtta
     1441 tggaacaagt agaacgttat ttgaaagaaa agagaataga atcatttgta aaagttgata
     1501 gtgggaatgg ctaccatatt tttcttccca taaatgaaca accaaataat cacgagacga
     1561 ttcttactat tcagaatttt cttcaattac ttcaccgaag atttggagta gaagatgaag
     1621 ttgatattga tacttctgtt tataatcctt caagattatg caaattagtt ggtacaccag
     1681 cggtcaaagg aaagtccaca aaagagcgtc cgcatcgttt atctagtttc ctttctattc
     1741 ccgataacat agatagaagt aattgtttta aaattcttga acagatagtt gaagaaaatt
     1801 ccatgatgtc aacctctaga gtaaaaagcc atccatcttt tagagaagtg ttacctttta
     1861 ttcaagcaga ctataaacaa tggttagctc actatcctga attaacttat accataaaag
     1921 agggcgatca taaaggagtt accttactta tatttgacga atgtccatta agaaaacaca
     1981 cgaataattc taatggttct tgtttgtctg ttacggctga taataagatt cgatttcatt
     2041 gtctgcatgc ttcggataaa aataaagata ttcaagactt tgtaaaaaaa tatccgttgc
     2101 caaaacctgt aaatccttct gtagaatcat tagaatccaa tcaagaatat tattttgacc
     2161 agtttaaact gaatcaagaa ggagtatttt tggtgaaaga aggtcaagaa gaaaaagttg
     2221 cttgtccgat gtttattaag caaatacgca aaaatatcga acaaaaagtc attacacttg
     2281 ttcttgccta ttatgtcgat cattgttggg atgaaataga aatagaaggt ggatgtttac
     2341 aaactaataa ttttaaacaa ttagcaaaac atggcttaat cattaaagct agaagagaag
     2401 ctgaagtcgt tgacttttta cagattcaac gggagaatgc tccaatcttt tttgaacatg
     2461 aatctcttgg ctggaaaaaa gaaaatgaag caatgatttt tcagttacaa gattcttttg
     2521 gacgtgaaat tccttctgta ttaactaaaa actcatgcta ccaattaaca actaatggta
     2581 cagaagcgga atttgacgca ttggttcata agtatgtttt aggaacgaac ttagaacttg
     2641 cttggtcgat tggtattgct tctattgtgt taggctattt aaacgttacg aaggctaata
     2701 cttttttgag tttactaatt aacctacagg gcaagagcac aacgggtaaa accactacgt
     2761 tacactttat tgcctcttta tttggaaatc caactacggt tcttagaaat atgaatgcta
     2821 ccaataaagc cattataaag ctagcatcaa acaatcaggg aggtgttcca ctaatactag
     2881 atgagttagg agcagcaact caaaataact ttagctcgtt gatctatcaa ttagcttcag
     2941 gagaagaaag gctaagatta gattctaatt gtcagcttat gccacaagag actttttctg
     3001 ttttgaccct tacctcttct gaagagcgtt tggaaaatta tttcaatgag aaattaactg
     3061 gtttgaaagt tcgatatatc gaattttctg atatcaagtt tacaaaggat gcgaatcacg
     3121 cagagaaagt taaaagtctt tcttctaata cttttggttg gggtataaag ttatttcttt
     3181 tgaaactctt tgaacaaggg attacaaaaa ttgaagcaac ttttgtcgaa gctcaaaaaa
     3241 aactatgggc tgttttacct gaacattctt tgaagcaacg cattggtaat aatctagctt
     3301 tgatttatag tggagccctc ctaattcaag atatactggg ctatccttta cgattagagc
     3361 atatacagac ggtgcttatt aattcgtacg aacagttaat tgtagatact agtttaggaa
     3421 gtgagcaaac ggtatttgac cgagtgaaaa cattgttggt gatgaatagc cataaatttt
     3481 taacaaacag tgacaacaaa catacgccca gtacgatttg gggaaaagta tcaattcaaa
     3541 aaaatgggga tattaaggta aatatttttc ctatcccgtt tgaggacttg ttaaggcgag
     3601 aatttcaagt aaaggatcta acctatcttt ttaggcaatt gcttgattcg ggtaatatgt
     3661 gtgcagaaaa agggagaaga acgaaacgta ttcacatgaa tcgtcaagtt cttccaacat
     3721 acgagattct attaccttct tcactcaaat cgtattttcg attgtaattt agaataaacc
     3781 aaagtatgaa agtgaggcac catgttatgg aaatatttct tattgattta gcaaaacaac
     3841 tgattactta tctgtatgat agcgagcaaa cccaaacgga agtaaatgaa gcttgatcta
     3901 tgcttcattt acttcattca atcaaacatg aaagaaagga aaattctaat gactaaaaat
     3961 aaaatgacac taaaagcaga ggttcttctg tatattcaag aacatttttc aaatcaagcc
     4021 ttttttacaa aaccaattta cttggatttc gaaataagag gggtatctgc tggatctatc
     4081 gggggtactc ttcaagcgtt gaagaacgaa ggatatttag aaaatcactt tgtgcaacgt
     4141 tcgttcaata gaagagtcgt gaaaaaatgg tatttagttc attcatagag aggggaatca
     4201 agcatgcgtg tggcttgtta tgatcgaacg tctgtttatg aagaagcgaa aattagtaat
     4261 gacgctcaat atgatgcagt gaaagagtat gttttagaga aatatgaaca aattccccaa
     4321 cgattttcag acaaaggaga atcaggggaa aaagcggagc gtccagcttt tcgacagctt
     4381 agagcttggg tttgtgcgtc agaagaagag ggggtgatct atgtttggcg ttatgatcga
     4441 ttggcacgtc aagccaaaat agccttagaa tttattgagt tgtgccaaca acatcaggtt
     4501 caagtgtatt cgatttctga acctcttcct gcttctttct cacacgcgtc aacgaataaa
     4561 ctatttgtgc aattattgtt cacttttgca gaacaacaac gaaacgtcat tattgagaat
     4621 gttcgtgccg gactttccta taaaaagcaa caacggcaat atttgtcttc tgcggtacca
     4681 tatgggtatc gtttagtgaa tgggaaaatc caacaagaag tacacgaagc aaggattgtt
     4741 cgctatattt ttcaacttta tttgaccaaa aaatatggct acaaaaagct ttgtcaacga
     4801 ttgacgcaac aaaagctttt cttccgcgaa cgtccttttc agccctatca tatttatagt
     4861 atcctaaaaa atccattgta ctatggcgaa atcaaaggag gatctttggg gaagtatcta
     4921 ggaacctttg aaccgattct ctcgaaagca acctttttac aagcccaaga gattcgccaa
     4981 tcaaggtgta cagctaaaaa agatacgtat ctgtacttat tacgtcaaaa aattcggtgt
     5041 cccttctgtg gtcgtcatct gagttcgaag tatcaatgga atacaaagaa gacgaagacg
     5101 cttcattatt atcattgtac cgaccgaagc tgccaaggaa tttttcttcg ggcagaaagg
     5161 gttgagtcag aagtattgga aaagatacag gctttttttc aatacgaagg cgtctatcaa
     5221 gccttgattg cccaattaac gcaacaatta gccgaaaagc gaaaagatga aaaacgcaaa
     5281 gtaatccaac aaaagaaaca agaagcacaa tggattaagg catttgaaga gggcaattgt
     5341 tcactggaag aactcaagca acaactgcaa gctcttcaaa agccgatgtc tacttcttcg
     5401 tttctttcac tccaacagtg tgaagcaaaa gtgaaagaat ggcttcaact aaaagacctc
     5461 tctagtcgac aattattatg tgactatgtt tgtcggattg aggtaacaaa ggataaaaca
     5521 gtttgtggga tttacttccg acaactgaca caaaatattt tagaaaggaa cgaatgatct
     5581 atggaaatga tacaaaccaa acgagtcgca ttgtatattc gcgtgagtac acaagaacaa
     5641 gctttagaag gacatagtat tcaagcacaa gaacaaaatg gacgacagtt tgccgaacgg
     5701 atgggctatg aagtggtaga agtttatgcc gatgaaggaa tcagtgggaa atccacaaaa
     5761 aatcggttcg cctatcaacg aatgatgaac gatgctcgga accatcgttt tgatctacta
     5821 gtgatttgga aattgacccg tctcggacgc aatatgttag atgtcttaca aacagtcgag
     5881 gaattattgc gatatgacgt aggattacat tcgatctcgg aacaatttga cgtgaccact
     5941 tcgacaggta aattgatgtt acaactcctt gggagtttcg gtgaatttga acgcaaccaa
     6001 attgcggaga atgttcaaat gtcaatgcaa agcttagtga aagataaaaa gcgttatgca
     6061 ggaggccgac gtttaggcta tgtcagtggt attgacacga atggtcaaaa acaattggtc
     6121 attgaaccag aagaggcgaa aattgttcgt ctgatctatg caaaatattt aggcggagca
     6181 gggtatcgag cgattgccaa ttttttgaat cgtcaaggat atcaaacagt caaaaagaat
     6241 gctttttcta ccacggcagt caaagatatc ttacgcaata aaatttatgc agggtattta
     6301 gaatatgcgc gctacatcaa ttgggaaacc aaacgacgga aggggaagaa tccgagaccg
     6361 attttagtga aaggaaccca tgaaccgatt atttcggaag aagattatca aaaagtacaa
     6421 gaacgccttg ctttagaagg aaaacaaccc aaatggaacc atacaggtga aaatgtattg
     6481 acaggcttgt tacgttgtcc agaatgtggc gcaccgatgg cagcgagtaa tgtcacaaat
     6541 accctaaagg atggcacaaa gaaacggatt cgatattatt cttgttcagt ctttcggaat
     6601 aaaggagcaa gtgtgtgtca tgccaattcg attcgtgccg aacaagcgga aacatttgtc
     6661 gccgaccgat taaaggagat cgttcagcta ccagaagttc ttccccgatt ggtggcagcg
     6721 ttaaatgaag aaatagtgcg acaaagccaa ccattggaac aagaactcgt ggtgttactg
     6781 gaacgaaagg aagaattgaa gactaaaatt gaaaaatggg aagctgcttt ggaagatagt
     6841 cctgaattgt ttccgatgtt aaaagaccgc ttagacgaat tgactgaaaa acgacgacag
     6901 ttgcacattc gagaaaatga aatcttaggg atctttcaac aaggagagcc gattcaagtc
     6961 aaagatgtcc aacggatatt gactagtttg gatcgttttc ttgcccagag tgagaagaag
     7021 caaatcaaag ccttgtatcg tacctttata gagaaaataa cctttgatcc taaccataag
     7081 gagatgttgg aaattacgat gaaattcacg catgccgtgg tacaacaatt gaatgagcaa
     7141 tatcaaatag ccgtatccaa aacaaaggat acggctattt ttgtgttaaa aacacccttt
     7201 acgttaacga tctagtctag gttatatcaa acaacgttag aggggcgcta acgcttctct
     7261 agcgatttga aatcgttaga gaagtagtta gggagg
//
LOCUS       DAYYGY010000112         7284 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000112 DAYYGY010000000
VERSION     DAYYGY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.115"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..995
                     /locus_tag="ACHA52_002646"
                     /pseudo
     CDS             96..995
                     /locus_tag="ACHA52_002646"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1230..1682
                     /locus_tag="ACHA52_002647"
     CDS             1230..1682
                     /locus_tag="ACHA52_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941302.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1696..2385
                     /locus_tag="ACHA52_002648"
     CDS             1696..2385
                     /locus_tag="ACHA52_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941303.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2510..4861
                     /locus_tag="ACHA52_002649"
     CDS             2510..4861
                     /locus_tag="ACHA52_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8941304.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            4941..5336
                     /locus_tag="ACHA52_002650"
     CDS             4941..5336
                     /locus_tag="ACHA52_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941305.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5661..6278
                     /locus_tag="ACHA52_002651"
     CDS             5661..6278
                     /locus_tag="ACHA52_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8941306.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6319..6618
                     /locus_tag="ACHA52_002652"
     CDS             6319..6618
                     /locus_tag="ACHA52_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8941307.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6652..6819
                     /locus_tag="ACHA52_002653"
     CDS             6652..6819
                     /locus_tag="ACHA52_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8941308.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(6864..>7284)
                     /locus_tag="ACHA52_002654"
     CDS             complement(6864..>7284)
                     /locus_tag="ACHA52_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8941309.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2505 a         1092 c         1456 g         2231 t
ORIGIN      
        1 aatgtagcaa ccggcatgac tgaggcagaa atcctagcga tgaaaaagga aatggcccgc
       61 atgcaggagg aaaacactat cctaaaaaag gctttaacca tattcgctca aaagtaaaaa
      121 tgagtgatgt cttcgaattt attcaatcaa atgttcatga tcatgacgta aaacaaatgt
      181 gtaccgtttt ggaagcaccg aaatccagct actatgactg gaagaaaaag aaaccttcca
      241 agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc aggaatctac tttgaaaaca
      301 agggtatcta tggtgcgcct aaaattcata agattcttgt tggccgtggc gagaccttgt
      361 ccttaaagaa agtacaaata ctgatgagaa aattaggctt gcgttcagtc accctcaaga
      421 agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg acgtaaaaac ctgttaaacc
      481 aagattttac cactacgtcg atcaatcaaa aatgggtgac agatattacc tatattcata
      541 ccctaaaaga tggctggacc tacctctcca ctattcaaga cctccacaca aagaaagtca
      601 tcggttggaa gtttggcaag cagatgacga aagaattggt tatcgaaacc cttgacaatg
      661 cgctcttaaa ccaaacaccc tccgagaact tgatcatcca ctcagatttg ggttctcagt
      721 acactagcga agcctatgag gcaaagctga atgaattgaa cattcggcac tccttcagtc
      781 gtaagggctg tccctacgat aatgccggta tcgaatcgtt tcacgcctca ctcaaaaaag
      841 aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc tgcttattta gcgttatttc
      901 aatacattga aggattttac aatcgcaagc gaatccatag tagtatcaac tatttaacac
      961 caaatcagat ggaggagttg gcattgcaag cttagttcct agctaccact tacatttttt
     1021 tggcgagatt tccacagggt ttcaacttca tcgcttcgat cagcggtcaa acggttttgg
     1081 tttgaccgct ggtcgaaacg atgagatact cacccgctgg aatctcgaaa aaattctcac
     1141 aaatcgtgtc cgaggtattg actcaaatcc accaattatc ttatcgtaga tatatattat
     1201 aaatttaata agaaagcgag gtgagaatcg tgaaaaaaac gatactaagt attttttcag
     1261 tatttacaat attaattggg ggctattttc ttttgaattt agattctgtt aattcaaacg
     1321 ctcaaataaa agatcaaaga gtagctgaac ataataataa aaaaatgagt ttgaccaaaa
     1381 atatctattc tatgtcagat gaagaattgt ataaagaatt tggtgattta tccggggaac
     1441 agttagaaga aagtattcca ggaattagtg actcagataa aattgttgtc ttgtctgctg
     1501 gtgggcaatt aatgggagat ggcaaatccg aattagttag ttataaacaa aatcacagac
     1561 agtttattga tgttaatact gattccaacg ctatttcgat taaggatcta aaagagcttt
     1621 taaatagtaa aagaaatgaa ttaaagcaga taaaagaaac taattcagtg gaattagagt
     1681 aaaggaagat tcattatgaa aaagattttt ggtgtttctg ttgggctact tatgatgtta
     1741 acatttggaa atgcagtata tgctgctgag aaagaagtta tttcagatcc agagcagtac
     1801 gcctcacgat actttgctga ttttgaagaa gaagataaag tacttgcatt gccagaaggt
     1861 ggttttttaa aaggggaagc taaaattgta tctatggata atccagatat aattcaaggt
     1921 tcatataatt cagaaactga tcctgcttca gtttcagtga cagaagcaaa aaaaattgtt
     1981 gctgaaaaat tcaaaaatga tattgaaact actccaatta tgcctcgagg agcatttcca
     2041 cccgatgttg gtactagcgg acaccttctt gtactagcag gaggaggaag ctataaatca
     2101 cagccattta gtggtgcggg atggcgtttt ggaggtatgg gtgtaaaacc agctgatggt
     2161 acaggcaact ttttacgttg gaaatcctac attgacgatg gaagagtagg aaatgtaaat
     2221 caagccaacg caactaaatc tggttctatt caaggaatgg cattgtatgc tcgtgaagga
     2281 ttcaaatact ttgcaccaaa cgggcctggt tggacaatga atataggtat ttattacact
     2341 tataatcctg ttgcaggtac gtattatgtt gttcaaaata tttaataaaa cagcacaaag
     2401 agatttcttt gtgctgtttt attattttta gttgttgaca aacactaaaa tataaaatat
     2461 aatcaacaca taaacgaacg tatgttcggc aagaaagcag gggattttta tggatgagat
     2521 ggttttaagt actcagaaat ggctaaacaa gaaatacagt aatgttacag ggtttgataa
     2581 ggttccagaa aatgggcgaa cagggtggcc tacaatctat ggacttattg aaggactaca
     2641 agtagaactt ggtattacta atctagtagc taattttgga cctaccactg aaaaaatgta
     2701 cgataatcag gttactccta aatggggcaa aaatctacct aaaaatattg tatttcttat
     2761 tcaaggtgcc ttttggtgta aggggattaa tccaggtggc ttcgatgggg tatatactcc
     2821 atatttagat actgctgtaa aagagttgca gactgatgca gggtttaatg gaagcgctgt
     2881 aactggagtt ttggatgcga aatgggccaa agcgttattt gatatgagtg cttttgttct
     2941 tgttcccggc ggagatagta aaattagaaa aatgcagcaa tggttaaatg taaattattt
     3001 agaatacacg ggaataatgc cttgtgatgg tatttatcaa agaaattcaa atcaagcatt
     3061 aatttttgca ttacaagctg aactaggata ttctcccagt gaagcaacag ggtcttatgg
     3121 taatgggaca actagtaaaa cttatcctgt gtctgaaggt aactcgggaa actatgtaag
     3181 aataattcaa tatggattat atgttaatgg ttattttgaa gacggtacat tcgatggtat
     3241 tttcactaaa tatatgggtt tagaagtact ggcattccga aaatttatgg tattgcctga
     3301 atatactgaa atagcagatg ttgttgtaat aaaaggacta ttgtctagcg ctggagatat
     3361 tagaagatct gcagatggcg cagatacttc tacgcaatta actagatctc aaatccaaac
     3421 attggttgat aatgatataa aaatagttgg tagatatctt actggtactg ttggaattgg
     3481 aaaagatgaa cgaaataagt atctaactac agaggaattg aataatattt tttctaaaaa
     3541 tttatctgtg tttcctatct atcaagatgg aggtgcagca ttagcttatt ttacttatga
     3601 tcgtggctta tcagatggta agaaggctat agatgcagca aaaaatctaa atataccatt
     3661 aacaacagtt atttattttg ctgttgatct agacatgtta ggggaagaaa ttcttgcata
     3721 tgctggagaa tattttaagg gggtatctgc tgtaatgtca tatagcgggt atcaaacggg
     3781 tgtttacgga acaagaaatg tttgttctca aataatcaat aacgggtatg cttcgtttgc
     3841 atttgtttca gatatgtcga ctggttatag tgggaattta ggttttccta tgcctagaca
     3901 atggtctttt gatcaatttg ttgaatttac cataggaagt ggcaacggag ctgttggtat
     3961 cgatttaatc gctacatcgg gtagagatag cggatttaat gagttatcaa atgatactaa
     4021 taatgataat tacattgcca aatacaatca gaaggttata aatcagatgg tacgtgcgta
     4081 tcaatatcta tcacaagctg gtcttgataa tccttggaac cctcatttga cattttatag
     4141 atatgtaaat tattctggtt tgtcatggga catcatttct tctcctgtaa cagagcatga
     4201 tagaaaaatt tatgatgaat ataggaataa attaactaat agcgagggcc tgtataatta
     4261 ttttatagat cctaatactg gttcaataat aggtttgcct cacttaattg tgacgttaca
     4321 atcacagatg tttctagccg acacaatcaa tgatagtgtt tctgactttg caggttggtt
     4381 aggagattta atgacttgct ggggtgaagt taagaaatta ggaatacaaa tggagcaagg
     4441 tgtatttgca ttagtaggta cggctgcagg tggaacattt agtctagaag atttatacca
     4501 agatgttgac gcggtaaatg tttatacctt agcaaaaaga aattctgatt caacagcttt
     4561 atcggcttta aaaacgtact attgtgataa gggtgctatt gatagattta atcaatttgt
     4621 taagaacaga tttggttctt cagataaaat cgcttcagta acaaatagtt tattagaaaa
     4681 tgacgaaaca gttgatatct taggtgtagg gataatttca tttttcattg ataaaatagt
     4741 taataattca cttgggacag attttactta tcttgatttt attggagatg aggggaaagt
     4801 tgcaaatgga ttttctgaaa aaattattca atttcaagaa aatagaagta aacttaatta
     4861 agaatcgttt actatttaaa cagtgtcctt ttccttttta aatatatacg ataaaataag
     4921 tatatgaatt gtaaaaggac atgatgatta tggaagaaat agaatggaat aatctgactc
     4981 aaaataataa aaataattct ggatgtggaa ttatctttat gtttgttata gttttgggaa
     5041 tagtagtact tttaataact gtcaagatag tagattactt tgcgaatact cctgaagaac
     5101 aaatacagag aaaacttcct gagtttgaaa gagtaccacc ttatgatcca agagataatt
     5161 catttcaagt aaaaaaatta aaagagtctc caaatgaaat gtggtttgaa gttacagttg
     5221 aatcaagcga cttcaaagaa aaaaagaaag aatacaatta tattgtaaag gcaaaagtat
     5281 caaaaagatt tatttttaac cctaaattag aaatatatag tgttgtaata gaataatagt
     5341 gttaaaaaaa ttgtaaaagt taaagaagtc aaattctgat gtgaaaaata aaatatacca
     5401 tggaagacat aaaagaaaaa ctggataact agtttattta atggcagacc gttaaggcct
     5461 gccattttta tgtattttag cggaataaat ataatcatgc gggagtgtaa acttactgtt
     5521 tcaaaaagta tactttttga catgccttga ttttctttgt ttatgtatgg cataataacc
     5581 acataataca atttgaaacg tttctgattt aatctgattg ttttgaaaca attttagtga
     5641 aaaaagggta gataaaaacg atgagaaaat ttttttatat ccgggtcagt agttatgatc
     5701 aatcgatcga acgacaactg atttccgcga aggaattagg aatccctgaa gaatatatat
     5761 ttgtagaaaa agccagcggc aaagatttta aacgccctga atatcaatta atgaaacgga
     5821 tgtttcgaaa aggagattta ctctatctac aatcattaga tagactaggc agaaataaac
     5881 aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa gcagattgat atttctgtgt
     5941 tagacatgcc tctgttagat actgctagtt acaagcaact agatgggtta gagacgctta
     6001 tctctgactt aattctgcaa ttactcagct atatggccga agacgagcgg aaacgaatta
     6061 atagccggca aagagaaggc gtagaagctg cgaaacaaaa gggtgtaaaa ttcggcagaa
     6121 agaaaattgc tttacctcct gaatttcctg cgatctattc tgactggaaa gcaggaaaga
     6181 taactgcagt ctatgctatg gatcaattgg gcatgaaacg aaataccttt tatcgacggg
     6241 tgaaagaata tgaacatacc ttgcaccact tttcttgata gatataacag ctattagacg
     6301 ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg tcgtttttac tggaacgctg
     6361 cagcaaatga cacgaaccca agccatcggt ttggttcact ctttaaacgg ccattgtcaa
     6421 tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc agtattctaa gtcgttgttt
     6481 gatgattctc tctatacaaa aaaggaacaa accgcgcggg acttgattgc gttaggtcat
     6541 gagattacca ttttatcaga agtcgaattt tatgcgttaa ttagtcaaca gttgaaagaa
     6601 tggcaacggt atttataatc gttagtaaaa tttacaagga ggaaaatttt tatgaagcga
     6661 aaatcttatg atcgttgggg cattgaaata actagtttta atcgaaatat tgcttttcac
     6721 gtggagtgtg actgtgggga attagcaaaa agtgtttatc aaaaaaatta cttttactgt
     6781 cctaaatgcc atcgccgata cggggtacat ggaacataga aacgaaatag tgcggtagaa
     6841 aagcgaatga atgtagtata tttctacaaa tcattcgctt tctttatttt ataaatagtt
     6901 ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg gaatgccttg ctttaacatg
     6961 tccaccacgg ttttaaaaac taagcgtttt tgcggatctg ttgagttaat agaatactcg
     7021 attggtcgtc ccttatatcg gccggctttt tgagccaata atactccttg ccgctgacgt
     7081 tcacgaattt tctcacgctc gttttgtgca atataactta gcagtgataa aaacatatcg
     7141 aacatagcgg tatccaggag ttcatttcca gtattaaaat ctaaaaactg tgcatctaat
     7201 atttttaacg ctactttttt ctgtttcata aaagcaactg tattttttat atcttcataa
     7261 tctcttccta aacgatctag tgat
//
LOCUS       DAYYGY010000113         7283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000113 DAYYGY010000000
VERSION     DAYYGY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.116"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..3319)
                     /locus_tag="ACHA52_002655"
     CDS             complement(170..3319)
                     /locus_tag="ACHA52_002655"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8941310.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="ACHA52_002656"
     CDS             complement(3333..4550)
                     /locus_tag="ACHA52_002656"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8941311.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="ACHA52_002657"
     CDS             complement(4540..6135)
                     /locus_tag="ACHA52_002657"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8941312.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="ACHA52_002658"
     CDS             6260..7045
                     /locus_tag="ACHA52_002658"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFM8941313.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7038..7280
                     /locus_tag="ACHA52_002659"
     CDS             7038..7280
                     /locus_tag="ACHA52_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941314.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         2132 a         1477 c         1199 g         2475 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aag
//
LOCUS       DAYYGY010000114         7144 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000114 DAYYGY010000000
VERSION     DAYYGY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.117"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1294)
                     /locus_tag="ACHA52_002660"
     CDS             complement(<1..1294)
                     /locus_tag="ACHA52_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8941315.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            1613..2338
                     /locus_tag="ACHA52_002661"
     CDS             1613..2338
                     /locus_tag="ACHA52_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941316.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            2328..2837
                     /locus_tag="ACHA52_002662"
     CDS             2328..2837
                     /locus_tag="ACHA52_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941317.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(2907..4355)
                     /locus_tag="ACHA52_002663"
     CDS             complement(2907..4355)
                     /locus_tag="ACHA52_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8941318.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4355..5071)
                     /locus_tag="ACHA52_002664"
     CDS             complement(4355..5071)
                     /locus_tag="ACHA52_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8941319.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(5052..5402)
                     /locus_tag="ACHA52_002665"
     CDS             complement(5052..5402)
                     /locus_tag="ACHA52_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8941320.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            5546..6319
                     /locus_tag="ACHA52_002666"
     CDS             5546..6319
                     /locus_tag="ACHA52_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8941321.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            6490..6856
                     /gene="ssrA"
                     /locus_tag="ACHA52_002667"
     tmRNA           6490..6856
                     /gene="ssrA"
                     /locus_tag="ACHA52_002667"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2088 a         1391 c         1273 g         2392 t
ORIGIN      
        1 atttggttaa aaatgttttg ccatgacgaa tcataattcg tagtttgaaa ttgtaaaagt
       61 tgcgaaagcc gtacgcattg cgttttaata ctttgatgtg gttgtttaag cattccaatg
      121 gtccgtttga atagctgtag tgaagcgcat tttggatctg tggcagaaac tttttaaacg
      181 ttcgtaaagt ggttgtatag gtttctggca gaccgctaat gtctctagtt aaacaatcct
      241 caaatagtga ctgatctctt ttctttaccg cataaagaat gtcttggtat aactgatagc
      301 cttggcgtaa gttgggacaa gcagctaaca gtcgatcggc aatctcactt tcagtcaaat
      361 aatctttaaa actggcacgc caaacccgtt tttcatagtt taactttgct tcttcttttt
      421 gtaggagttt ccaatagcgc ttcaattttt tccctaaccc tcgttgagag tgggaatttc
      481 ctttaagtcg taccgtggtt tctttcactc gatgattcaa gaaagttctg ccaatatgtt
      541 ggacgatgtg gaaacgatcg atgatcaact gagcattcgg gaaaagcttt ttcaccaacg
      601 aaacataagg cgcatacata tcaataacaa tcaattgaac ggcttcacga gtcgctaatg
      661 aaaaccgaga aaaatagcgt tctaaataag gcaagcgtcg attttcgaca atatccaata
      721 atgcatggct ttgtccgtcc atcatgataa aactcattgc actggcaaca cttcgaaccg
      781 atttgaattc atcaaagcat aagacctttg gcaaagtctg tttgaaagga ttcaatgggc
      841 ggtagaatcg cttcatgaca cgatagaccg aagaagtgga aatatttttc tgacgagcga
      901 tttccgtcat ggaagtattc tcttttaagc gagcaatgac agaccaacga actttttgac
      961 taatgaaaca gtgtttttca gcaacagagg tatccgctac gaatgttctt tggcaagact
     1021 tacatttaaa tcgttgtttc ttcagctcca gataagtgcg atactcagac acatcattca
     1081 gtaacaaccg aacggttgtc gtcccccaac gaatgatcct agaggggacg acacaaagac
     1141 aatgggaaca ctcttctggc ttgtacagaa gagttccttt gtaaatcaag caaatttgcc
     1201 ctttgtattt ttctttagtt aaacaatttt cctcgaaata gatatttagg tctagaatat
     1261 ttagggtatc tttgataagc tgagtatgag acatcttgaa tcctcctatg aattggtagc
     1321 caaattaatt ctaaaggatt tcagggtgtc ttttctatat ttttttatac aaaaaagatg
     1381 ttggtgaagt tcaccaacat caaaaagtat agagcccaaa attattgtcg ctgctcctct
     1441 tttcgaataa acagtgttca aaagcttgca tctacggtaa tagaataggg cagcttcttc
     1501 acaacctctt gctttttaca aaatattttc tggttttaat tcgtgatttc actaaaggaa
     1561 tttcatactt tattcatatt cattttctat actttttata gaggtgaaaa gtatgaaaat
     1621 aatccgctat gctactgctc gattaattta ttacaaaaag cagacaatac tctattgttt
     1681 attttcaagc ttttctgctt ttttattgat agcatgcctg actctttacc attcgcaatc
     1741 ggctttagcc aatcaagtcc aagatcggct ttcatttatt ggcgcttctg catctacaga
     1801 gcttcttccg gctttagaat tagctagccg attttatata aaaggcatat tcagtttatt
     1861 ttttctattt gccttccttt ttatcttttt cttttactat tcagtcaggc aaaacaagca
     1921 ggaattgatc aattggcgcc tgactggttt ttctaaaacc aaattattcc tttttatttt
     1981 ctggcagctg ttctttcctc tctttctttg ttgtatcttg gttttattgt ggatcgtcag
     2041 cttccagtca gtttatgaag cgcttcttca aaggatcaat tttcttgtct taaagactac
     2101 tgaactgcca gatatccgtg atatcttgac ttcggtaagc ggacttacaa ttccgatgga
     2161 tcaacagact ttttttacca tcgatttcca aaatgatctt tttttcacag atatacttaa
     2221 gggatttgcc caaactctta ttgggctcag tggatgctgt ctcattctta gtctattccc
     2281 tttcactttt tttatccatc atttgcaaag taggaggcct ggacgtaatg aaacctgaat
     2341 atttgattca tgctgaacac ttgaccgtta ttttgtgtcc tctcgaaaaa cagcataaaa
     2401 tattggagca cttgaaaaaa gaggttcttg ttgcaaaact ggataatttt gcgatcatcc
     2461 aaaaaaattg gccgatgttt ccttatctga atcttaaaga ccatgtcctg ttggatgtac
     2521 cggaaaaaga aatcaaacaa gatcgtttgg cctatcaaga aaaacttgat atctctcctt
     2581 ccttgcttaa ttgtgcggca gctgagttga cgtcttttga aaaagtaaag ctgcaattac
     2641 ttcatgcctt gctttccaag cgaaacaata ttgttattga agatacattt gatgaactta
     2701 gtgtattgga gatccaagag ctactccact tgctttctta tttagctcat gaagaaaatc
     2761 aaggtatctt actctttact catgatgcta ctattgctca aagtccttat attgatcgtt
     2821 tagagcccgc gggctaaatc aatcatttta tagtaaaaac aagcggtata gaagatcgat
     2881 tcatgatctt ttataccgct tgttttttac tcttttttga tttctacgtc aggcaaacga
     2941 acagcgaatt ttgttccttt tcctagttcg ctttctactt ctaattttcc tttatgcaag
     3001 cgtacaagtt gctgcacgat tggtaaacct aatcctgatt caccatattt tgtatttttt
     3061 cttgaaggat ctactttata ataacgatcc cagatacttt tcatttgttg ttcagacatt
     3121 ccaattcccg tatcttcaat cgtgatgatc gtttcaagat agcctttttc taaagtaatc
     3181 gtgatttggc cgttttcagt aaactgtatc gcgttttgaa taatattgac catgatttgg
     3241 acaaatcggt cataatcagc gtaaacatca atatcatcaa cggctttcaa tgtcaatgta
     3301 tctcctgctg cttctgcttt agcttctaac tgtgcaacaa tatttttcaa tgtttctgtt
     3361 ccattgaatt ttttaatgac catactgatt tgattcgtac ggattttttc ataatccaag
     3421 ttttcattca ccaaacggat cagacgctct gtttcatttt tcatcaattt aatcgcgttt
     3481 tctcgctgat tttcagggat tgcattgtat tctaaccctt ccagaagtcc gttgattgtc
     3541 gtaagaggtg ttctcatctc gtgagaagca tctgccataa attgtcttcg acgattttct
     3601 tgttcttcaa tttctgcttg agattctttc aatgaattcg tcattttatt gaaatcatct
     3661 gctaactcat caaattcatc tttgtcatgt acggggagct gtacatcaaa attaccattc
     3721 gtcacttctt tcgtcgcatt ccttaaccga ttgatacgct taacttggaa tgctgcaaaa
     3781 gcatagctgg caataactgc aacgatactg gaaaaaataa atcctttgaa caggttcagt
     3841 gtaattggac gaacgctgtt atcgatattt cgcgcaggtt gactaactac aagtgctcca
     3901 tagaattcat gattcaaatt aaatggcaca agtgcgtaag aggtcgcctg tgcttgtcct
     3961 aaaatatttt tattcgatgt gaatttttct tgacgtccat tcctcagatt tttccattga
     4021 tcagctgtta aggtaaaatt cagtaaagct gtattagtcg gataaatgac acgttcgttt
     4081 ttatcgataa atataaagtt cacattttgt tctgtcagta cttgctctgt cagcagcaat
     4141 gcattttgaa acgcctgatc ttgactaaac tgcgggagcg tatcggcata ggtttgcgtt
     4201 gtcttttcaa ctgattcagc atatccaaac agttgctggt aattgttttc ttcaatcgtt
     4261 tgttttgtca gttgtgtaaa agaaactcca acgatcaata agatcaacgc gaccattccg
     4321 caaaaagcta agagttgctg atacaaataa cgcattaggc aacaccagaa tcatcgaatt
     4381 tgtagccgac tccccaaaca gtttggatga cttgtggtcc gaccttttcg attttttgtc
     4441 gtaatttctt gatatgggcg tccactgtcc gttcatcacc aaagtactga taatcccaga
     4501 ctaattcaag caactgctcc cgagagaata cttgacgtgg tttttttgct aaagtaaaca
     4561 atagatcgaa ctctttaggc gtcagtcctt caattggccg gttgtccaga tacgcttctc
     4621 gtgttttcgt attcatcttg aaatgactag tctcgatatc aaaatgatca tctgaaactg
     4681 ctgcttcttc tcttgcttct gccaactcac tacggcgatg caaagctttc attcgagcaa
     4741 taagcgtcag aggactgaaa ggttttgtga cataatcatc tgcccccatc tctaagccga
     4801 tcacctgatc gctttcagaa tctctagctg tcagcatgat aatcggcaca gtacttgaaa
     4861 ctttgcggat ctctcttcct actagcatac cgtccagtgt gggcaaattc aggtctagtg
     4921 taatcatatc ccaatgttgt gggttttcta aaaaggcatc gagcccttct tttccatcat
     4981 acttgaaagt tgcttcccaa ccttcattta agaaaaacat ctgcatcatt tcagaaacag
     5041 attgattatc ttcaatcatt aaaatattca ttatcgttcc ctccagtttc caggtcatat
     5101 ttctgtaatt tcttttctgt cataataaaa ataggcataa ttggaaatac tagcagaagc
     5161 atttcaatca gaaaataatt cgtatgccca gatgtcttta aataatagcc gatcaatgcc
     5221 atcagtaagc ccataagaaa aaaccagttt ccgcttgtct tttgagccag ccgccaatga
     5281 gcgtctgttt ttgcggcaag aggcgaatga tacccataaa aaggcagatt tccttttgag
     5341 ggcagaatcc gaaaaagact gccaattatt aagaataaga taccaatact gaaaaaaatc
     5401 attctttccc tcctaaattc tcttcttata gtatactcta aattgttgac tgaaccaata
     5461 tgttcaggaa ttcttcacga aaggtttgtc ttttgttcat tctttgttaa gataaactta
     5521 ttccaataaa aaaggagttt tatttgtgtc aaattatcgt gcaatcactt tctttgatct
     5581 tgatggaaca ttactagatg cgaaatcaaa aatcacaccc gaagtggcaa aagctatgaa
     5641 cgcattaaaa gaaaacaatg ttctccccgt catcgctaca ggacgtacag aagcagaaat
     5701 ccactctatc atggaagatg cgggcattac ttctgcggtc accatgaatg gttctttcat
     5761 tcgagtagac ggcgaagaaa tctattctca aacattttct tcagaagaat gtcaaaaaat
     5821 gtatgatcat gtacagttga acggtcatga actttcttac tataacgatc agaaaatctg
     5881 gtgtaccggt catagccaaa caatgatcaa tgcttacgac ttcattcatt cagatgttcc
     5941 ggaaattgat cctttaggct ttaaagaaaa cacagtaaat atgttgctga tcttatctca
     6001 atcaggtgac gaatactatc acgaacattt tcctgagcta actttttatc ggaatggtcc
     6061 ttactcgatc gatacagtga agaaaggaat ctcaaaaggg gcaggtgtca aacgtctgca
     6121 aaaagaactt cagctggaaa aagttcctac ttttggattt ggcgatggtc cgaatgattt
     6181 tgccttgttg gaagcttgcg acaacaaaat cgctatggac aacgcctatg acggattaaa
     6241 agaactttct acctttatca caaagaaaaa tacagaaggc ggaattgtcc acgcattgaa
     6301 gtattttgat ctgatttaag atcttttttt gaagctacct cttttcttaa gaaaatcgct
     6361 tattttttct taagaaaggg gcagttatta tataaataat gggatatgat tcgctttttt
     6421 attgaattta tcccaatata tgctatacta attttgtatg aaaaatacac gtaacccttg
     6481 ttagctaacg gggacgttac ggattcgaca ggcacagtcg agcttgaatt gcgtttcgta
     6541 ggttacgtct acgtaaaaac gttacagtta aatataactg ctaaaaacga aaacaactct
     6601 tacgctttag ctgcctaaaa acagttagcg tagatcctct cggcatcgcc catgtgctcg
     6661 agtaagggtc ctaactttag tgggatacgt ttcaactttc cgtctgtaag ttgaaaaaga
     6721 gaacatcaga ctagcgatac agaatgcctg tcactcggca agctgtaaag tgaatcctta
     6781 aatgagttga ctatgaacgt agatttttaa gtggcgatgt gtttggacgc gggttcgact
     6841 cccgccgtct ccatatatgt atggttgaga cagataaaca tgtatgaaaa acgttgttaa
     6901 atcaacgttt ttttattttg gtcaaggaga gaaaagtaga gaaatacaaa attttttgca
     6961 ttgattttgc attcaaaaaa acattcaaac atttagaaat ccccaatacg tattcaggaa
     7021 aatctatttt aaggtttaca aaaagaactt ttgttcgtac actctttttg aggtgaactt
     7081 ttatgctgat ggaaggaaaa acacaattat ggtttaagca tgttccttca aataagtttt
     7141 ttta
//
LOCUS       DAYYGY010000115         7141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000115 DAYYGY010000000
VERSION     DAYYGY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.118"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACHA52_002668"
     CDS             <1..615
                     /locus_tag="ACHA52_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8941322.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACHA52_002669"
     CDS             612..1385
                     /locus_tag="ACHA52_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8941323.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACHA52_002670"
     CDS             1382..1780
                     /locus_tag="ACHA52_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8941324.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACHA52_002671"
     CDS             1965..2636
                     /locus_tag="ACHA52_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941325.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACHA52_002672"
     CDS             2695..3135
                     /locus_tag="ACHA52_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941326.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACHA52_002673"
     CDS             3147..3815
                     /locus_tag="ACHA52_002673"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8941327.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACHA52_002674"
     CDS             3879..4502
                     /locus_tag="ACHA52_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8941328.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACHA52_002675"
     CDS             4558..4944
                     /locus_tag="ACHA52_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8941329.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACHA52_002676"
     CDS             5157..6548
                     /locus_tag="ACHA52_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8941330.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DAYYGY010000116         7118 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000116 DAYYGY010000000
VERSION     DAYYGY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.119"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..219)
                     /locus_tag="ACHA52_002677"
     CDS             complement(<1..219)
                     /locus_tag="ACHA52_002677"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8941331.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="ACHA52_002678"
     CDS             complement(330..584)
                     /locus_tag="ACHA52_002678"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8941332.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /gene="prgO"
                     /locus_tag="ACHA52_002679"
     CDS             complement(777..1052)
                     /gene="prgO"
                     /locus_tag="ACHA52_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8941333.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="ACHA52_002680"
     CDS             complement(1024..1977)
                     /locus_tag="ACHA52_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8941334.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="ACHA52_002681"
     CDS             2589..4082
                     /locus_tag="ACHA52_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8941335.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..4513
                     /locus_tag="ACHA52_002682"
     CDS             4217..4513
                     /locus_tag="ACHA52_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8941336.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4537..5496)
                     /locus_tag="ACHA52_002683"
     CDS             complement(4537..5496)
                     /locus_tag="ACHA52_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8941337.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5679..6530
                     /locus_tag="ACHA52_002684"
     CDS             5679..6530
                     /locus_tag="ACHA52_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941338.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6551..6763
                     /locus_tag="ACHA52_002685"
     CDS             6551..6763
                     /locus_tag="ACHA52_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941339.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2362 a         1150 c         1286 g         2320 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
     4501 gtatatccag tagtagtttt tatttactct taattgtcaa attaagttag acaaatcatc
     4561 aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg attttctagg
     4621 aatattattt cgtttcgatg cgacagattg aataaaagat tcatctactg aattgaaatc
     4681 catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt ttaggcctct
     4741 ttgagacggc gttcctggat cagcgaaata aatggcaata tcattggcat tactgatctg
     4801 tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata gatttttcgg
     4861 tacagattca aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc taccacacgg
     4921 tttcaatgtg atgataactt ttgataatcg ctcaactaag gtaattacag cacttttatg
     4981 tttcaaacct acgatagtgt caccttcaag gtgaccaaac tcatttgaga attggctata
     5041 atctttttca cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc tttcttgatg
     5101 tccatttggt ttacgcttgc ctttcatcgg taagtcagaa ggattaaata gccccttttt
     5161 gaacatacga taaatggtac gagcagaaca acgaatagga aacgctgcac gaccaacaat
     5221 cacatcgggt gtccatcctt gaacaacctt tctttgaata tattctgatt gttcctcagg
     5281 taaaacaagc gggcgtctac cacagtttga tttgtttttc ttatactgtt gatagtactc
     5341 taaggctgat tttccttgct tgaagaacag gtatacttta tggatcgttt gtcttgaacg
     5401 attcaagaaa tgcgcagttt tcgcaacaga ttgatttatt ttgaaataag actctatcat
     5461 tacaagttca tccgttgtaa gatgggtata ggtcatttgt actcactcct tataattctt
     5521 tggacggaac tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta
     5581 caatcggcgt acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag
     5641 gaaaacgtta aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg
     5701 aacacacctg gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat
     5761 tatggccatt aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt
     5821 actcgattgg agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag
     5881 catcagacat ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta
     5941 ctcaattagt catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc
     6001 tattcgattt aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat
     6061 ctggaagaac aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg
     6121 gacacatgta tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga
     6181 aacttattaa acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg
     6241 gttatgagta caatgttgat tttggaagat atgacgaatt gatcaatacg gatatcgttc
     6301 acacgagtat ccatgaatct actgttaagg gcatcagatc tattttaaaa aatggccagg
     6361 aaatgaaagc aatagatcga agaaaagaga tctacaattt ttatgaagtc gaagcaagcc
     6421 tacgatatgg catcggaaaa ttgtggttag atggaagtaa tattcgtcaa aacaaaccat
     6481 acattttttg gaacagtagt gatttagaac ttccaatgaa agataaataa ttatggagaa
     6541 tcgtttgaaa atgattcctc tatacaggat tgtgcttata gttggcatgg agttaaaaac
     6601 cttaaagaag gagcagttat tcaaagtgaa agaacgtcat aatcagttac tatgtagtaa
     6661 aacgggtacg atgaacattg aataccgaac agaatattta gaattaacga atcaaactga
     6721 ttttttgttg attcgaaagc aacaagagca agaaaaaaat taatggctac ttatagccaa
     6781 ttgattgatg aatgggaaca attcaaggaa tctaaaattc cagaacgtct ctaaaagcct
     6841 ttatttgtaa agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg
     6901 ctgtgcctat tacgtttctc aaaaataaat gtgggaacaa aatatttaaa aataggatag
     6961 tgaaaggata gtgaaggata gtaaaaggag ggtagtagga tagcaaaaag gataggctcc
     7021 ttttcgctat cctttttaaa ttggaggaat taatatggaa gtaagaataa gaaatgtgga
     7081 tgagaaaatc gtaagagaaa ttgatcgaag atgtgacg
//
LOCUS       DAYYGY010000117         6935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000117 DAYYGY010000000
VERSION     DAYYGY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.120"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1151)
                     /locus_tag="ACHA52_002686"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="ACHA52_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="ACHA52_002687"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="ACHA52_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8941340.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="ACHA52_002688"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="ACHA52_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2435..2665)
                     /locus_tag="ACHA52_002689"
     CDS             complement(2435..2665)
                     /locus_tag="ACHA52_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8941341.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2895..3713)
                     /locus_tag="ACHA52_002690"
     CDS             complement(2895..3713)
                     /locus_tag="ACHA52_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8941342.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="ACHA52_002691"
     CDS             3874..4563
                     /locus_tag="ACHA52_002691"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8941343.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="ACHA52_002692"
     CDS             4577..6079
                     /locus_tag="ACHA52_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8941344.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="ACHA52_002693"
     CDS             6092..6568
                     /locus_tag="ACHA52_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8941345.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="ACHA52_002694"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="ACHA52_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2251 a         1093 c         1265 g         2326 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataacctt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatg
//
LOCUS       DAYYGY010000118         6925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000118 DAYYGY010000000
VERSION     DAYYGY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.121"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..777)
                     /locus_tag="ACHA52_002695"
     CDS             complement(25..777)
                     /locus_tag="ACHA52_002695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8941346.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1141..3807)
                     /locus_tag="ACHA52_002696"
     CDS             complement(1141..3807)
                     /locus_tag="ACHA52_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8941347.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4148..5599
                     /gene="cls"
                     /locus_tag="ACHA52_002697"
     CDS             4148..5599
                     /gene="cls"
                     /locus_tag="ACHA52_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8941348.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5928..6743)
                     /gene="thiD"
                     /locus_tag="ACHA52_002698"
     CDS             complement(5928..6743)
                     /gene="thiD"
                     /locus_tag="ACHA52_002698"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8941349.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         1975 a         1469 c         1196 g         2285 t
ORIGIN      
        1 taataaaatc ataaaaagaa cccttcacga gtcgtgaaag gttctttttt tatctgcgga
       61 ccaaaggagc agtctatagg tcagcttagt cgtactaagg atttctcgtt tttcactgaa
      121 tagtttgcga atgtacctgg tcacaacagg atcttttgcg tacgcgtcat agtatagctt
      181 catcaattct tcagtaattg attcctgtgt aaaataagta aaatcttttt tcttcacttt
      241 gaatccttca tcaattaagt atgtttcaaa ttgatccatt acaactggcg atgtattagg
      301 atcttcttct atcattccta gttgcttttc gataggataa aaaacatcat cgattgattc
      361 cactactctg attatcacgc accatttgct tgactttttc atcaattttt ctaattgaat
      421 aagatgtacc aatgctggat ttgctgctgt aaaaaccatt tcatatgtct catttgattg
      481 gataaattct tctaacgact gtttgataaa ggaaacattt ggcaactcat ttttttctgc
      541 ttcttttttt gcattttcta gcattcctga agaaaaatcg atcaaatcat agcgattaat
      601 ttcttttgct aaggaaagaa atctgccaga tcctccagca acgtctgctg ctgtttcagc
      661 aggcaggact ttctccgttt tcagataatt ttttacatct tcagcaatag ttacttgcga
      721 ttcttgctgg atttcataat attcttcagc aaattcatcc cattcttttt cattcatttg
      781 atttcctcta attttttgtt atatgttttc acttttcgga ataaatcatt ctcctctttt
      841 atctattcta tctcttttga tgaataataa aatgattggc tttccgtcta tcataaataa
      901 aaaaagggac tgtgaccaaa gtcgtgtcaa agtccctctt tccgaataaa cagtgtccaa
      961 tcagatgacc ctcagtatta gctcaaaatg ctgattttac aacttcttcc ttaaacggtg
     1021 ttctcctgcc aactcttcgg tcacaagtca caatattcaa caaacatgag aagctgtttg
     1081 ttgaaattgc accttgtgac tcagaattaa acggcaggct cacaacctcg ttgattcgtt
     1141 ttatttattt ccttgtactc gtacaatgat caatttagtg atttccatca aaacaattgt
     1201 cacaagtgcg attcccatac atgtaaagac ttctgtcaat ccaaatgctg atgggataga
     1261 gaagacttct ttcagtcctg ggatcattgt taagcagtaa agcgcgctac atacaactac
     1321 agctagtaag acacttctgt tagagaagaa ccctgcttgg atagctgttt gtgtgtttga
     1381 acgtgctggg aatgtttgca atgtccgaca ccaaatcaat gttgaaaatg acatggctac
     1441 accgaattca ggtgaatgct gcatgccaag ccattgagca aagataacag caatcccaat
     1501 cagcacacca cgatacgtta cagaaatcaa tgttttgtcc gtaaagattc ctgaatttgg
     1561 atctcttggc gggcggttca tcgtagcagg ttcagctttt tctgtaccta atgcaattgc
     1621 tggcagtgaa tcattgacta aattgatgaa caacagttgt aatgctgtaa atggattgga
     1681 ccagtcagca actaaggcaa agatgatcgc aatgatcgca cctaaatttc ctgcaaatag
     1741 ataagaaata gctttcttga tattatcaaa cacattacgt cctacttcta ctgcgctgac
     1801 aatagaagca aagttatcat ccgtcaatac catcgctgct gcatcttttg ccacatctgt
     1861 acccgttccc atagcaattc cgatgtctgc ttgtttcaat gctggtgcgt catttacacc
     1921 atcacctgtc atagctgaga tatgaccttt attttgccaa gcacgaacga tacgaatttt
     1981 attctcaggt gaaacacgtg cataaacagt gactttttct aaaacttcgt ttagttgatc
     2041 ttctgtcaat tcatctagtt ccgtcccggt caaagccagg tttccttctt caaaaatccc
     2101 gatttgtttt gcgatagcaa cagctgttgt tttgtggtca ccagtaatca tgattgtttt
     2161 gattcctgca cttcttgctt ctgttaccgc ctgagtcact tcagcacgtg gaggatcgat
     2221 catagcaagc aaaccaacaa ggatcaaatc gtcttcatct tcaaatgata aatcaaaatt
     2281 atcaagtgga cggtaagcaa aagctagtac acgtaatgcg cgtgttgaaa aagcttcatt
     2341 ttgctcttgg aattttttca tcaattcatc cgtcatcgga acgacttcac catttagcaa
     2401 tactttttta ctgcgttgga agacgatatc tggtccgcct ttaacaaaca agtaattttt
     2461 gttatcaatt gtgtgtccag tagacattaa ttttcgatct gagtcaaaag gaagttctgc
     2521 ttgtcttggg tagttgtttc ggatttcctg ataaggctgg tttaattttt cactaaagtc
     2581 gataaacgct acttccgtag ggtcgcccaa tttagtaccg tcttcactga tcgatgcatc
     2641 attcgctaaa acactgattt cgatcaagcg acgttcgtca gctgtccatt ttgaagggtc
     2701 atttgtgaaa tccccagtat cgccattacc taaaaagtag tcaacaactg tcattttgtt
     2761 ttgtgtcaat gtaccagtct tatctgtaca gatgatactt gtcgaaccaa gtgtttcaac
     2821 tgctggaagc ttccgaataa tggcatgacg ttttgccatt ttattcgttc ccaaagataa
     2881 cacgattgtt acgatagact gtaatgcttc tggaatagca gctacggcta cggctacggc
     2941 aaacatcact gcgctgacga tatctgcagt catatctcct gtaccttctc caagataaat
     3001 acgaaacagt tggataccta aaatcaataa agacaatgcc aaaatagcca aacttagttt
     3061 tttactgaat ttatccagac ctcgttgtaa cggagtgact tgctcagttg ttgattccaa
     3121 aaggccagca acttgtccga tctccgtatt attcccagtt gaagtcacta agaatttacc
     3181 gcgtccgtaa gttacgatcg ttccactgaa taccatattt gtacgatctc cgattggtac
     3241 cgtacttgtg atatccgtta cttctttttc tgcagaagtc gactctcctg tcagcatacc
     3301 ttcatctact tttaatgatc ccgcatctag taaacgtcca tcagcaggta cataatcgcc
     3361 tgcgtctaac aacacgatat ctcctaccac aagttcgttt gccggaatcg ttttttcctt
     3421 accatcacga atcactcgag catcgggcgc tgaaaggttt ttcaacgctt ctaaagaacc
     3481 ttctgctttt tttgtttgga caacactgat tatagaattc aacatcaata ctgcaaaaat
     3541 aactaagaat tcgatgtagt cacccatgac catctgaatc agtgcgacaa tcagcaatac
     3601 gataaccatt gcatctttga aagtttctaa gaacaatttc caagttggct tcttcggctt
     3661 ttcttttagc tggttcaagc catctctttc taatttttcc ttcgcttgcg tggtgctcaa
     3721 tccatcggtt gagctctgcg tttgctgcat caacgagtcg atcgtttctt tatagaccgc
     3781 ttgttgtgct tgttccttgt tttccaaatt tcatccccca ttttttgatc ttggccctag
     3841 tcctgtctga aaaaaaagag acctaagaca aatacgcatt tgtcttaggt ctcaccattt
     3901 catacaatac cagaaaacca tttggttaac ttactgttga tattatcgca acaaacgttg
     3961 ccggttactc ccctaaggag atattcaact gtactaatat cctaccttat taacctacaa
     4021 aaagcaacta ctttagcccg atttacctac attttcatga aaggagtgaa aagtatattc
     4081 accttggtag gcttctttct tctatcatgc tatactaatg ttattgtatg taaggaggaa
     4141 catttttgtg gtatctagta ttataaccgc cctttatcta ttaaatgcac ttattgcttt
     4201 ggtggctatt ttgatcaaac cccgagatgt agcagccatt tgggcatggc tcttagtatt
     4261 gtttgccctt cctggggtgg gctttgtatt atatttattt ttcggacgtg gattaacgga
     4321 taagaaaaaa ttctatctcc gacaaagtga cttgaaagaa ttagaaaact ttcagtcttt
     4381 tagaggagat accattgaac attacgatcc tgacatgggc gataaagaca aacagcaatt
     4441 tgttgacttc ttctcttcat taaatcgtat gccgctgaca agaatgaatt ctgtcactct
     4501 tctcacagac ggacaagaga aattggattc actgcttcaa gatctaaaaa aagccaaaca
     4561 ttcgatccat atcgaatatt acgcatttgt gacagataat atcggccagc aagtcttaca
     4621 tgttttagaa gaaaaagccg cagaaggcgt ggaagttcga atattatatg atgcatttgg
     4681 ctctcatggc acaaaagcaa aagatttcaa tcgtctaatc aaaaatggtg gacatgtcca
     4741 tacatttgtt acctcacaaa gggcattact tcgtttccga ttgaattacc atgatcaccg
     4801 aaaaatcgtt gtgatcgatg gaaagattag ttataccggt ggtttcaata ttgccaatca
     4861 atatgtaaat acaacaaaaa aattcggcta ttggcgcgat acgcatatac ggattttcgg
     4921 tgccgcttct tctttgctcc agcttcgctt cttaacagac tggaacgtct cggtacctga
     4981 agaaaaaaag gtcggctatc atttgaatta tttctttaaa aaagcagatc gagatgaatc
     5041 taagcttgct gatacatcca tccagcttgt ttcaagcgga ccgaataacg aaagggaaca
     5101 aatcaagctt tcatttatca aattgattac ttctgctaaa aaacgtgttt ggatacagac
     5161 accttacctt gttcctgatg aaagtgtcat tgctgcttta aaaatcgcaa ctgcctctgg
     5221 tgtagatgtg aaaatcatga ttcccaacaa accggatcat ccttttattt atcgagcaac
     5281 acaatattat gctcggcagc tgatcaagga aaatgtacaa atccttgtct atgagaacgg
     5341 cttcctccat gcaaaaacat tgataatgga tgatgaaatc tgcatggtag gttcagcaaa
     5401 tcaagatatt cgtagctacc gattgaattt tgaaacaagt gctgtcattt acgatcctga
     5461 gtttttagaa gaacttgcta ctcagttcaa agaagatgag acacattgtt catccatgac
     5521 aactgaaaca gtcaaggaaa tgtctaactg gctattattc aagcaacaaa tttctcgatt
     5581 attttctcca atcctataat ttttttattc aagtagagaa aaatatacta agattaaagg
     5641 gactgtgaca aaaatcttgt cacagtccct tcttccgaac aaacggtgtt ctcctgccaa
     5701 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgtttttt gaacttgttc
     5761 cttgtgactc ggagctggac ggcaggctca cgacctcttt cttaaacggt gttcaaaagc
     5821 ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agaagctgtt tttacttggc
     5881 tgttcttatt acttgatgca gggcaacttt ttcacaactt ctttctttta ctctaattca
     5941 ccagctgccc aatgattgat ggggccatat ttgtgcccta catttatttc ttcacgaatt
     6001 gcacggttta catagtcttt tgccaatcgg atcgcttgtt caactgtttt tcctttcgct
     6061 aactcagctg caatacatgc agatagcgaa tcgccagttc cgttgatccg gtcggtagga
     6121 taaaatggtg catttaacca aaatgatttt cctgattcga acaatacgta gtcattgact
     6181 gtttcttgct gcgcattttt agcatgatgt cctttgatca taacgttttt tgcacccata
     6241 tcttgcagtt cttttgctgc ttgcacaaaa tcttcttcag ctgctaatgt ccgtccagtc
     6301 agcttttctg cttcataaaa attaggtgtc agtacttcgg ccaaagggac aagcttttct
     6361 ttcaagcttt gcaaagcaga ttcttctagt aaaagatttc catgtttcgt tacgattacg
     6421 ggatccacga ctaatggtcc aaaatcatat ttttggtaat tttttacaac tgtctcaatc
     6481 aactttgaat ctgctagcat acctgtttta gctgcacgta tttgaaagtc cttcgcaatc
     6541 agttcaaatt ctttgtcgat aaaatctgtt ggcaatgtca cacttgctcc gattccgtaa
     6601 gaattacctg caacacatgc ggtcatgaca gaaacaccgt aaacatttct tgtaaagaat
     6661 gtatgcatat ctgcttgcat ccctgcgctt ccatcgctat ctgatcctgc gatcgtcatt
     6721 gcttgtacat actgatcttc catcaaatcg attcctccat ttttataaaa agatttttca
     6781 taatactgtt actgtttcta tcatatgttt ttcaaagtat ccctcagtaa atataccact
     6841 accttttctt ggtaatcaaa tttatttgaa aaaacatttg caataaatca gaaaatagtg
     6901 gtcgtttatt tgtattcgct attct
//
LOCUS       DAYYGY010000119         6766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000119 DAYYGY010000000
VERSION     DAYYGY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.122"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..275)
                     /locus_tag="ACHA52_002699"
                     /pseudo
     CDS             complement(72..275)
                     /locus_tag="ACHA52_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            271..438
                     /locus_tag="ACHA52_002700"
     CDS             271..438
                     /locus_tag="ACHA52_002700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941350.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(481..1056)
                     /locus_tag="ACHA52_002701"
     CDS             complement(481..1056)
                     /locus_tag="ACHA52_002701"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8941351.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1211..1737)
                     /locus_tag="ACHA52_002702"
                     /pseudo
     CDS             complement(1211..1737)
                     /locus_tag="ACHA52_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2194..3705
                     /gene="gadC"
                     /locus_tag="ACHA52_002703"
     CDS             2194..3705
                     /gene="gadC"
                     /locus_tag="ACHA52_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8941352.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3718..5118
                     /locus_tag="ACHA52_002704"
     CDS             3718..5118
                     /locus_tag="ACHA52_002704"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8941353.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5283..6718)
                     /locus_tag="ACHA52_002705"
                     /pseudo
     CDS             complement(5283..6718)
                     /locus_tag="ACHA52_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2085 a         1168 c         1221 g         2292 t
ORIGIN      
        1 ctttttacca ctccaaacag ggagcgtcaa taattttgtg taaataactc gtccttctgc
       61 aaaataatgg gttactcagt aaacattgaa gctaatgtat ccgtcacttg ttggaagcct
      121 ttatggcttc tatttaagaa tttttgattg tatgtatcaa agatggatac taggaaacgc
      181 tctagtgatt cttcattttg aaactgctct tttctgcggc tgtatttctt gatttgttta
      241 ttgaaagatt cgattgtaga accagaagga atggagaaca cttttgaact ttacaacaaa
      301 actactgttg ataaattaat tccaatttta gttccaactg atttaaatgg tcatgatgtt
      361 ttacttcgtt ttaacggatt tttaggtgag gattatagat acctaaaaat ttctgatcct
      421 caacctttaa cagaataata aaaaacgtgt aatcaattat ttgattacac gttttttatt
      481 tcaatcttcg ttgtagtagt ggttgcgaca taccgaaagg taatcttcat tacctcctac
      541 tacaatctgt tcacctgaat aaactggaat accatcaaca atacgagcat tcattacagc
      601 ctttttattg caaaaatggc aaatagtttt aatttcctca attttatcag caaatgtcaa
      661 taatgcttcg gatcccttga agagattatt ttgaaaatca ttcttcaatc catagcaaat
      721 tactggtact tcctcattca ttacgataag tgatagatca ataatattct ctctacttaa
      781 aaattgagac tcatcgacca aaatacaatc aacttttccg tactgatcaa taatctccac
      841 taattctccg tacacatcca tttcagaagt aattgcaata gctggtttcg aaacacctat
      901 acgactctca acttttccaa atcctgatcg agtatctatt gcgcttgtca ttatgataac
      961 tctcttccct tgttcttcat aattatgagc cactttcagt agctcaatac tctttccact
     1021 attcattgtt ccatatctgt aaaataattg tgccattaaa tttattccct tcttttacaa
     1081 ttcaattatt ccttaattcc atctgctttt atgcagctgc tcgtttattc cttgatatgt
     1141 ctataacatc tactatccct aaaaccaatt tataagccat tagagatggt attcctgaac
     1201 atctctcata ttgttttttg agtggtatat catctgaaaa actgaaggaa tcatgaaaat
     1261 agcgacatac ttatcattta gagttctaac tttaaatgca tttacttctt cagtcagcac
     1321 tttagtcatg ttggacatgg tttggggcgt gtagtgatgc ccatacattt tctcaatcaa
     1381 gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa
     1441 tgtgtcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcgc cattgcggtc
     1501 gcgcggaatc tggagatgaa gttccccata ctcggtcttg accgtacggt cataggagcc
     1561 gttacgagaa ttacccgtgt taaaaccaat gcgatcgtac ttttcgtaat cgaggaaagc
     1621 cgttaactcc gttttgagaa gcgtattgac agctttttcc aaatggacac ggaataatcc
     1681 attcaaatcg cctttgttcg ctagagtctt taggatttct gtagtaaaat cgttcatatg
     1741 gaagtcctct tttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt
     1801 tttctattta cacaaaatat tttacactct caacaaaagt atcttcgatt aatacttgct
     1861 caagttatgt gcagctacga aaaagtaggt accgaggtac tttctttttc gcagcttttt
     1921 ttggacttat gagaagtaca ttcgctggaa gaatacgata taaaacgagc tttttcacta
     1981 aagatattaa tagagtgaaa gaaacaagct tttgttgtca gttttttcac ttttctaaaa
     2041 atgaaaataa aaaattctgg aagtggagaa gaattttgct tgctatactt tgtcttgtag
     2101 aaaagaggta gacaaaataa tgcaagctca aataagcgat tttccataac cttggtagga
     2161 atgacaaatt aaataggaaa gggaagtaat aatatgaata aaaaaaagtt atctctattc
     2221 ggtttttttg ccctgacagc ttccatggtt ttgactgttt atgagtatcc gacctttgca
     2281 acatcacaat tacatttggt tttctttctt cttttgggag gtattttatg gtttctacca
     2341 gttgctttat gtgctgcaga aatggcaaca gttgaaggat ggaaagatgg tgggattttc
     2401 agttgggtta gtcaaacttt aggagaacgt tttggttttg cagctatttt tttccaatgg
     2461 tttcaaatta cagtaggttt tattacaatg atctacttca tactaggagc gctgtcgtat
     2521 gtaattaatt ttccagagct taatacagat ccatttatca agtttattgg tgtattagtc
     2581 attttctggg tactgacttt ttcacagttg ggcgggacca atcgtaccgc taaaattgct
     2641 aaagcaggat ttgtcattgg aattgttatt ccttcgatca ttctctttgg tttagcagca
     2701 gcctacttta tcggcggtaa tccgattcaa atggctatta atgcgaaagc ttttattcct
     2761 gacttttctc agctttcaac tttagtagta tttgtatctt ttatattggc ttatatgggc
     2821 gtagaagctt cagcatcaca tattaatgag ctggaaaatc cccaaagaaa ctacccattg
     2881 gcaatgtttt tactagtgat tttggccatc gccttagatg caatcggcgg tttctcagta
     2941 gcagcggtta ttccacaaca tgacttgtct ctcagtgctg gtgtaatcca aacatttgaa
     3001 acgcttattt tacattttaa cagtcattta cattggttgg taaaagtgat tgctctcatg
     3061 attgcctgtg gcgttatggg tgaagttagt tcttgggtag ttggtccatc acgagggatg
     3121 tttgcagctg ctcagcgagg tctgttacca gttttcttcc ggaaaactaa taaacaagac
     3181 gttccagttc cgttgattat gatacaagga attatcgtta ctatttgggc agcagtctta
     3241 acttttggtg gaggaggcaa caatttatca ttcttagtag caatctcctt aactgttgtc
     3301 atttatctcg taggatattt actatttttc ctaggttact ttgtattagt ttttaagaaa
     3361 aaagacttaa aacgaactta caatgtacca ggaaaaactg ttggcaaaac gattatcggt
     3421 ggaattggtt ttattttatc agtgttcgca ttgcttatct cttttgtacc accagcttca
     3481 attgcagcta gtgagcaccg aacataccaa atcatactac ttattagctt tgtagtgact
     3541 gttcttttac cttttatcat ttatgaagta catgataaaa gtgctcacac aactattgag
     3601 gaacctactc acgtgaaagc tgaagacgtt aatcccgctg tttatccagc tgctagagga
     3661 gagcaccacg ttacaaaaaa tgatgaacat attttaaaac attaggagga attcattatg
     3721 ttatacggaa aagataatca agaagaaaaa aactatttgg aaccaatttt tggctctgca
     3781 agtgaggatg ttgacttgcc taaatataag ttaaacaaag aatccattga accacgaatt
     3841 gcttatcaat tagtacaaga cgagatgttg gatgaaggaa atgcgcgatt aaacttagct
     3901 actttttgtc aaacgtatat ggaacctgaa gcagtgaaat tgatgaccca aacgttagaa
     3961 aaaaatgcaa ttgataaatc agaataccca cgaacaacgg aaattgaaaa ccgctgtgta
     4021 aatatgattg ctgatttatg gcatgctcca aataatgaaa aattcatggg aacttcaacg
     4081 atcggctctt cagaagcctg catgctgggt ggtatggcca tgaaatttgc ttggcgtaaa
     4141 cgtgctgaaa aattaggtct tgatattcaa gcaaaaaaac ctaacctggt gatctcttct
     4201 ggttaccaag tttgttggga aaaattctgt gtatattggg atgtggaact gagagaagtc
     4261 ccaatggatg aaaaacatat gtcaattaat ctagatactg tcatggatta tgtggatgag
     4321 tacacaattg gtattgtagg tattatgggt attacttaca ctggtcgtta tgatgatatc
     4381 aagggtctga atgatttagt tgaagctcac aataaacaaa ctgactataa agtatacatt
     4441 catgttgacg ctgcatcggg tggcttttat gcaccattta ctgaacctga tctagtttgg
     4501 gattttcaat tgaaaaatgt tatctcaatt aattcttcag gtcacaaata tggtttggta
     4561 tatccaggtg tgggttgggt cttatggcgt gaccaacaat acttaccaga agaattagta
     4621 tttaaagtta gttacttagg tggagaaatg ccaactatgg ctatcaactt ctctcatagt
     4681 gcagcacaac taattgggca atactacaac tttgtacgct atggctttga tggttatcgt
     4741 gatattcacc aaagaactca tgatgttgct gtttatttag ccaaagagat cgaaaaaact
     4801 ggtatttttg aaatcattaa tgatggatca gaattgccag ttgtgtgcta taagctgaaa
     4861 gaagatccca atcgcgaatg gacactatat gatttatctg atcgtctgtt aatgaaggga
     4921 tggcaagtcc cagcctaccc attacctaaa gacttggatc aattaattat tcaacgctta
     4981 gttgttcgag cagactttgg aatgaacatg gctggtgatt atgtacaaga tatgaaccaa
     5041 gcaattgaag agttgaataa agctcatatt gtttatcata aaaaacagga tgtgaaaacg
     5101 tacggcttta ctcactaaaa ttcttcagag aaatataaaa gaagttaatt aactaataaa
     5161 aggagttgtg attaccacac agctccttaa atctatttga ataaatatat gatattcatt
     5221 tgttacactc tcaatcttag ctgttgttct attcatcatt actcttattg taagtagttc
     5281 agtcaaattt gcttgttcaa ttgatcgata acagtatgaa ttgtttgaaa agtaatcgga
     5341 tatataattg ttttctcact acctaagcct tcttggtata aagaactatc gcaaagtata
     5401 atccgctgag tacaaaagaa agtgtcgtct actatttctc taagttgttt acaaatgata
     5461 ttactaatag aaaagtgttc tcccaaaatg tcttctaact gttgtcgata gtataaatac
     5521 ctaaagtctg acggagctac tatgaacact tcaattggag atttccgttt atgctcattt
     5581 aaaagactta aactaatggt tagtttacga atagaattct cattccaacg cagaaataaa
     5641 ttgttttgtt ttcgccattg ttcaagaatt tcatgaactt ctaagtacaa agcgcgttgc
     5701 tcttcattta acaagtaagt cttatcaggt tgaaatatct gaatatcagc ccaaagtgag
     5761 cggataaacg ggagaagtgc tttatcaaaa agtatctgat tttcttcaga tatattcaga
     5821 gataagataa gtttttgact gagctcaatc accaaagggt tatctgctac atggttttga
     5881 taaacaacgt tcaagtcttt ttgtaacaat tcaaagttat cgcatagata atttctagag
     5941 ttgaatagaa agtagatata aaggcattca tcttcgtcat gacataaaga gaaagaaagt
     6001 tgctctaatc ctttctgtat tagctgaaac aagggagtat gtcgggcaac tcttttttct
     6061 tcctgactaa aatatattgg gttagtctta gatctctgca aaccgatagc gatacctaat
     6121 aaaataaatt gtttttcaac tgtagggtaa cttcttgaaa aaaatgtttt tctacataat
     6181 caatcaattg ttgaccgctt tgaataattg atttatttag ctggatcaac tctgtccgat
     6241 ttgtctcata gattatcgtt agtaacaaac ttcgataatc cttttcagga atttcaaaat
     6301 ggccatcagc atttaagtac tgcatttgtt taaaaaaatt aatcaactca tttcgtaagt
     6361 tgtaaacttt gcttaacgaa atgaaggtag tatcagctat attactccaa ttgtgttccc
     6421 ctgataaata atgtcctagt acattgaaaa agtcagagcg ctgatagatg agttgagtga
     6481 gtgccaacct cgaaaacgaa gagtcgaatg cgactgtaaa gctacttcta gatttctcca
     6541 ctgaaataat ttgctgatgg agtatgtcgg taatgcattc caagtcgtta gtgacagtga
     6601 gtgggcaaac gttcaattga tctgctattt cgcgaatctg aatggtccct ttttcgtgta
     6661 attgctcaca aatgtaaact tgacgaaaaa tacttttctc cagataggtt tccaacatag
     6721 tactcctcct agtcatttta tacataacta ttataactct accaat
//
LOCUS       DAYYGY010000120         6481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000120 DAYYGY010000000
VERSION     DAYYGY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.123"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA52_002706"
     CDS             complement(99..1229)
                     /locus_tag="ACHA52_002706"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8941354.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHA52_002707"
     CDS             complement(1878..3080)
                     /locus_tag="ACHA52_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8941355.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHA52_002708"
     CDS             complement(3140..3343)
                     /locus_tag="ACHA52_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8941356.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACHA52_002709"
     CDS             complement(3485..4549)
                     /locus_tag="ACHA52_002709"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8941357.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHA52_002710"
     CDS             complement(4610..4717)
                     /locus_tag="ACHA52_002710"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941358.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHA52_002711"
     CDS             4880..5692
                     /locus_tag="ACHA52_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941359.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHA52_002712"
     CDS             complement(6066..6257)
                     /locus_tag="ACHA52_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941360.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1957 a         1318 c         1013 g         2193 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 a
//
LOCUS       DAYYGY010000121         6045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000121 DAYYGY010000000
VERSION     DAYYGY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.124"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..1079)
                     /locus_tag="ACHA52_002713"
     CDS             complement(117..1079)
                     /locus_tag="ACHA52_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8941361.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="ACHA52_002714"
     CDS             complement(1081..2520)
                     /locus_tag="ACHA52_002714"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8941362.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="ACHA52_002715"
     CDS             2705..4651
                     /locus_tag="ACHA52_002715"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8941363.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4915..5787
                     /gene="scrK"
                     /locus_tag="ACHA52_002716"
     CDS             4915..5787
                     /gene="scrK"
                     /locus_tag="ACHA52_002716"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8941364.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1910 a         1038 c         1069 g         2028 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     4741 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa
     4801 aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat
     4861 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
     4921 tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg
     4981 acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc
     5041 attgacttct tcaaacaata ccaagcagat ttagcaggga ttgggattgg ctcatttggg
     5101 cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaattg
     5161 gcatggcaaa attttgattt catcggtacc atgaaaaaag aatttccgat cccgatttct
     5221 tggacaacag atgtcaatgc ggctgcctat ggtgagtatg tcttcggcag tggcaaagga
     5281 ttgagcagtg tcgtttacta tacgatcggt acgggtgttg gcggcggtgc cttacaagaa
     5341 gggcgcttta tcgaagggtt tagtcatcca gaaatggggc atatgctcgt cgtccctcat
     5401 ccaaaagatg actttgctgg cagctgtcca tttcatggca actgtttaga agggatggcc
     5461 gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac aagaggttgc agaagatgat
     5521 ccttattggg aaatcgaagc ctcctatatt gctcaatgtg cctacaatac aaccttgatg
     5581 ttttctccag atgtgatcat ttttggcggt ggtgtgatga aacaacgcca tatgctgcaa
     5641 aacgtccacg acgcatttgc aaaattggtc aatgggtatg tcgaaacacc tgagttaagc
     5701 aaatatatcg tgaccccagc attagaagac aacgctggaa cgctcggttg tttggcattg
     5761 gctcgagaag cggtcttaca ttcataaacg aaaaaaggct cttcgacatt tagtgttggt
     5821 agagaaagat acaactgaaa atttagtcat aaccagttag gcagagtgct taactggttt
     5881 ttgttttact tggaagatca agccttgaat gagataaatt cttgctcgaa agttatggaa
     5941 atttcgatag ccgtaggcca cttgtattgt gcaatagttt tttacaaaaa aaagggggtg
     6001 cggatatttt tttggacctt tttaacaaat atatctttgc ttctt
//
LOCUS       DAYYGY010000122         6011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000122 DAYYGY010000000
VERSION     DAYYGY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.125"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHA52_002717"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHA52_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8941365.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACHA52_002718"
     CDS             complement(2944..3378)
                     /locus_tag="ACHA52_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941366.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="ACHA52_002719"
     CDS             complement(3395..4600)
                     /locus_tag="ACHA52_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8941367.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="ACHA52_002720"
     CDS             5294..5680
                     /locus_tag="ACHA52_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8941368.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="ACHA52_002721"
     CDS             5774..5965
                     /locus_tag="ACHA52_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941369.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         1959 a         1063 c          829 g         2160 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa t
//
LOCUS       DAYYGY010000123         5226 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000123 DAYYGY010000000
VERSION     DAYYGY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.127"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..1107
                     /locus_tag="ACHA52_002722"
     CDS             133..1107
                     /locus_tag="ACHA52_002722"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8941370.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="ACHA52_002723"
     CDS             1089..1550
                     /locus_tag="ACHA52_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8941371.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="ACHA52_002724"
     CDS             1600..2064
                     /locus_tag="ACHA52_002724"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8941372.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="ACHA52_002725"
     CDS             2078..2827
                     /locus_tag="ACHA52_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8941373.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="ACHA52_002726"
     CDS             2827..3657
                     /locus_tag="ACHA52_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8941374.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="ACHA52_002727"
     CDS             3657..4304
                     /locus_tag="ACHA52_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8941375.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="ACHA52_002728"
     CDS             complement(4402..5178)
                     /locus_tag="ACHA52_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8941376.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1782 a          706 c          941 g         1797 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaaa tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tattgtaaag ttatatgact ttataatact tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtatcaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgcatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttaataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgacttca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgc taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtaaat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaa
//
LOCUS       DAYYGY010000124         5212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000124 DAYYGY010000000
VERSION     DAYYGY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.128"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            454..954
                     /locus_tag="ACHA52_002729"
     CDS             454..954
                     /locus_tag="ACHA52_002729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941377.1"
                     /translation="MNYNKNLLSHIVYFNYIIIGVYHLNLTQRRGYFETQKENLSRSP
                     LFLDDWNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTA
                     GYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWD
                     NTITNW"
     gene            927..1370
                     /locus_tag="ACHA52_002730"
     CDS             927..1370
                     /locus_tag="ACHA52_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941378.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="ACHA52_002731"
     CDS             2540..3508
                     /locus_tag="ACHA52_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8941379.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="ACHA52_002732"
     CDS             3525..4049
                     /locus_tag="ACHA52_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8941380.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="ACHA52_002733"
     CDS             4425..4706
                     /locus_tag="ACHA52_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941381.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGR"
     gene            4708..5124
                     /locus_tag="ACHA52_002734"
     CDS             4708..5124
                     /locus_tag="ACHA52_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941382.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1967 a          895 c          867 g         1483 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaaatcagc
      361 gcctggatcc cttggtacag gcgttacttg gtagtatgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga agataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DAYYGY010000125         5007 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000125 DAYYGY010000000
VERSION     DAYYGY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.129"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHA52_002735"
     CDS             complement(<1..182)
                     /locus_tag="ACHA52_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8941383.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHA52_002736"
     CDS             517..951
                     /locus_tag="ACHA52_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8941384.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHA52_002737"
     CDS             974..1294
                     /locus_tag="ACHA52_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941385.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHA52_002738"
     CDS             1435..1626
                     /locus_tag="ACHA52_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941386.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHA52_002739"
     CDS             complement(1697..1954)
                     /locus_tag="ACHA52_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8941387.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHA52_002740"
     CDS             2112..2375
                     /locus_tag="ACHA52_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941388.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHA52_002741"
     CDS             2399..2587
                     /locus_tag="ACHA52_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941389.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHA52_002742"
     CDS             complement(2670..2855)
                     /locus_tag="ACHA52_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941390.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHA52_002743"
     CDS             3070..3441
                     /locus_tag="ACHA52_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8941391.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHA52_002744"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHA52_002744"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHA52_002745"
     CDS             complement(3709..4137)
                     /locus_tag="ACHA52_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941392.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHA52_002746"
     CDS             complement(4197..4955)
                     /locus_tag="ACHA52_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8941393.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1742 a          784 c          978 g         1503 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaattctag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt attttta
//
LOCUS       DAYYGY010000126         4955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000126 DAYYGY010000000
VERSION     DAYYGY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.130"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1154)
                     /locus_tag="ACHA52_002747"
     CDS             complement(<1..1154)
                     /locus_tag="ACHA52_002747"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8941394.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1339..3780
                     /locus_tag="ACHA52_002748"
     CDS             1339..3780
                     /locus_tag="ACHA52_002748"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8941395.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4110..4814)
                     /locus_tag="ACHA52_002749"
     CDS             complement(4110..4814)
                     /locus_tag="ACHA52_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941396.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1552 a          915 c         1039 g         1449 t
ORIGIN      
        1 tatattttgt agtaacgaat atcattttcg atctttgtaa tcgaaaaagg tacatctgaa
       61 gtatattctg cacattctcc gtgttcagga agcgtctcac tgaaaatcgt ttcatattcg
      121 tcaacagtga tccgtgttcg agaatctagc atctcttgat gagattgtgc gaagagatat
      181 tcttgatagt tttcttccag ataacctgta aaaaattcac ttactgcacc agaaccgtag
      241 ctgaatagac cgatgcgatc tcctggctgt agtgatttcg aattttccag tagagaagtt
      301 agccccaggt ataatgaacc agtgtaaaga ttaccgattc gtcggctgta acggatgctt
      361 tcttcatagc gagccataag acgttcctga ttttcttcgt ctgtttggtc taatacgctt
      421 tgcaatgcct tttttcccat cttagtatac ggaatatgga aagcaatcgc ctgataatct
      481 tcgagtcctc gacccgacaa ttctttatgt cgattccaaa ctttttggaa tgattcgata
      541 tacgtagaat tagataaagg accatcaaca acaggaaatt cgctataatc tggacgccag
      601 aaatcataga tatcttctgt cagaaatacg ctgtcgtctt caatcgataa aatacgcggg
      661 ttttgagtga tcatcatcgc aacagcaccg acaccttgcg tcacttcacc accgcttgcc
      721 aagccgtaac gagcaatatc acttgctatg actagtactt ttcgttctgg atgatttttg
      781 acatattctt tcgccatgtg caaagctgcg gtgccaccgt agcaagcctc ttttacttca
      841 aaagaacgag caaaagattg gattttcagt aaatggtgga taattaccgc actcgctttt
      901 gaatgatcaa tcccggattc tgttccgacg atgaccatgt cgattagctc acggtctttt
      961 tctgttacga ttttgctggc agcatttgct cctagtgtaa tgatgtcttc atttgcacga
     1021 ttgactgcca tttggtcttg accaatccct atatggtatt ttgcaggatc atccccacgg
     1081 ctttctgcca actctgtcat atctaaatat aaattaggaa taaaaaagga aagacgatca
     1141 atccctattt tcatttcaac acctctttac ttatgataaa aaacttactt tactaaggta
     1201 tcataaaaag attaaaagaa atataaaaaa gagaatattt aaggattgtt aaaatttaat
     1261 cttagaaaaa aataactgga acaagagcta tttgttttta cagcaaagaa tggtatattt
     1321 ttgtaggagg tggcttactt gaaagaagtc gttatgatag atgctgcaag aacgccgata
     1381 ggaaagtatc gcggaagttt aagtccattt actgcagttg aattaggcac acttgtgact
     1441 aaaggattat tagataaaac aaaactgaaa aaagataaaa tcgatcaagt aatatttgga
     1501 aatgtcctgc aagctggtaa tggtcaaaat gttgccagac agattgcttt gaacagcggt
     1561 ctccctgtag atgtcccagc aatgacgatc aatgaagttt gcggatctgg tatgaaagca
     1621 gtgatcttag cacgccagct gatccaatta ggtgaagcag aacttgtgat agccggcgga
     1681 acagagagta tgtctcaagc acctatgctg aaacagtatc agtcagaaac aaatgaatat
     1741 ggtgaaccaa tttccagtat ggtcaacgac ggattgactg acgcattttc aaatgcacat
     1801 atgggattaa ccgcagagaa tgttgcaaca caattttctg tgagcagaga agagcaggat
     1861 cgctatgcct tgtcgtccca gttgaaagca gcacatgctg tcgaagccgg tgtattttct
     1921 gaggagatca tcccagtcaa gatttctgat gaagacgtgt tatctgagga tgaagcagtt
     1981 cgtggaaata gtacattgga aaaactgggc acgttacgta cagtattctc agaagaagga
     2041 actgtaacag caggaaatgc ttccccgttg aatgacggtg cctctgtggt gatccttgca
     2101 tccaaagaat acgcagaaaa taataatctg ccttatttag caaccatcaa agaagtagcg
     2161 gaggtcggta ttgacccaag tatcatggga atcgctccaa tcaaagccat tcaaaaattg
     2221 acagatagat caggaatgaa tttatcaaca atcgatcttt ttgagatcaa tgaagcattt
     2281 gcagcttctt ctatcgtagt tagccaagaa ctgcagttag atgaagaaaa ggtcaatata
     2341 tatggaggag caatcgcatt aggccatcca attggtgcaa gcggtgcacg tatcttgact
     2401 acattagctt acggattgct tcgtgaacag aagagatacg gtattgcttc attatgtatt
     2461 ggtggaggtt taggcttggc agtcttgtta gaagcgaata tggagcaaac acataaggat
     2521 gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac ggcgcagtca attaatagaa
     2581 aaaaatgtat tgacacaaga aactgcgttg atttttcagg aacaaacact ttctgaagag
     2641 ctctccgatc acatgatcga aaaccaagtg agtgaagtgg aaatcccaat ggggatcgcg
     2701 cagaattttc aaatcaatgg caagaaaaaa tggattccga tggcaacaga ggaaccatct
     2761 gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa atatctgcgc agaaacacct
     2821 cagcggctga tgcgagggca aatcgttcta tcaggaaaat cggagtatca agcagtgatc
     2881 aatgcagtta atcatcgaaa agaagagctg attctttgtg caaatgaaag ttatccttca
     2941 atcgtgaaac gcgggggcgg tgttcaagat atctctactc gggaattcat ggggagtttc
     3001 catgcatact tatctattga ttttttagtg gatgtaaaag atgcaatggg cgctaacatg
     3061 atcaactcga ttcttgagag tgtggcaaac aagttacggg agtggttccc tgaagaagag
     3121 attctattca gtattttaag taattttgct acagaatcat tggcttctgc ctgttgtgaa
     3181 atcccattcg aacgcttagg gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc
     3241 caacaggcag gggaatatgc gaagcttgac ccttaccgtg cagcaacgca caacaaaggg
     3301 atcatgaatg gtatagaagc tgtagtggca gctacaggaa atgatacacg tgcggttagc
     3361 gcttcgatcc acgcctacgc ggcaagaaat ggattatacc aaggattaac agactggcag
     3421 atcaaagggg ataaactagt tggaaaacta acggtgcctt tggctgttgc cacagtaggc
     3481 ggagcttcca atatcctgcc aaaagcaaaa gcatctttag ctatgttaga tattgattca
     3541 gccaaggaac tagcacaagt aattgctgca gtagggttag ctcaaaacct ggctgctttg
     3601 cgtgcattag ttacagaagg aatccaaaaa ggccacatgg gacttcaagc tcgttcatta
     3661 gcgatctcaa ttggggctat tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa
     3721 gcagaaaaga tgaaccagca gacagctata caaatattgg aaaaaatccg ggaaaaataa
     3781 aaaagcaata tctttagaag aataaagcaa gatttacagt tattttattt actgcagatc
     3841 ttgctttttt ctctatcaga aaatatgttt attattagaa ttttttatag ttaaggttag
     3901 gcatcttaag aaatcaggca actccaagat atcttcaggt agaagagaag tcgcctaaca
     3961 ttgtaggatc ataggtgatt tgttggacag gtcaagctga tatttgagcc tgatgttaga
     4021 aggaatttct ttttacttgc tgtagaaatt attatatgac taaaaaggct gggattttaa
     4081 atcccagcct tttatgacaa atagaatttt tatcgatcag taatcgctgt cgctgcaaat
     4141 tgttcgatca aaggaatcaa agtcaatttg actttgtatt tccgttcgag tgctttttcg
     4201 ataacgaact cattttcagt agtcatagaa atagttgcta atttacgagg ttcatcagca
     4261 tttttttcat taataactac ttcatgttca ttttgttctg tgtaaaattt aggatactca
     4321 gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta
     4381 ttttcagttg tgtccgcttt tggtttttcc gttttttctt ctggcacact gactgtttct
     4441 tctgtaactg gtgtcgcaac agcttgcgca gttggttcat cgtttttcaa tcgttgatca
     4501 ttccagtaag acacgatttc aataagaaga ttgtcacgga atcgttcgaa aatcccgttt
     4561 tctcctgaac gaattctctt taatacagta ttgaagttgt catcgattgg cacgttgatc
     4621 cgtgtatctt catttgtttt cccagctaat ccgatcgtta agatttgacg gtttttggga
     4681 tcaagcatga cttgtaacgg caaaccataa cgtcgcaagc tctcttcttc tagagaacgg
     4741 aataattgtc ctcttacacc atccaatgaa ggacggaatt gatttaataa tttttgtttc
     4801 tgttttttct tcatatgttc tctccttcgc tcataccctt tcgattataa gaaaaaaaca
     4861 gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat
     4921 ttgcgtattt tcccgttttt tgaaagaaaa ataaa
//
LOCUS       DAYYGY010000127         4805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000127 DAYYGY010000000
VERSION     DAYYGY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.131"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1199)
                     /gene="nagA"
                     /locus_tag="ACHA52_002750"
     CDS             complement(51..1199)
                     /gene="nagA"
                     /locus_tag="ACHA52_002750"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8941397.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1275..2111)
                     /locus_tag="ACHA52_002751"
     CDS             complement(1275..2111)
                     /locus_tag="ACHA52_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941398.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2295..3512)
                     /locus_tag="ACHA52_002752"
     CDS             complement(2295..3512)
                     /locus_tag="ACHA52_002752"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8941399.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3661..3822)
                     /locus_tag="ACHA52_002753"
     CDS             complement(3661..3822)
                     /locus_tag="ACHA52_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941400.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3895..3978
                     /locus_tag="ACHA52_002754"
     tRNA            3895..3978
                     /locus_tag="ACHA52_002754"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3929..3931,aa:Leu,seq:gag)
     gene            complement(4218..>4805)
                     /locus_tag="ACHA52_002755"
     CDS             complement(4218..>4805)
                     /locus_tag="ACHA52_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941401.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1459 a         1001 c          775 g         1570 t
ORIGIN      
        1 ccttaaaacc aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg
       61 aaacgttcca cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata
      121 aagtctgcat cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt
      181 gctggaatca gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca
      241 acatttttaa ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga
      301 cgtgctgttc cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc
      361 atcccaccgg ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc
      421 attaggattc ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag
      481 acatgttcta aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc
      541 ataccattga aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct
      601 tctagtgtag cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg
      661 acaaactctt ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct
      721 gaagcttctt gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt
      781 tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta
      841 gctcctggtg cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct
      901 ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca
      961 gacatcactt tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga
     1021 atatgtgtat ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact
     1081 tctccctcag gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag
     1141 cctgcatgtt tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt
     1201 ctattctctc cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga
     1261 atggtctaca ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca
     1321 tagaatgctg ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat
     1381 aaatcattga ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc
     1441 tgttctaacg cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa
     1501 atttgcgaaa attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt
     1561 cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata
     1621 caactaatca gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag
     1681 cttaatgctt ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg
     1741 caaacaccga gataataata atagcgttga aagtcagttg cttgatacag attttcagaa
     1801 atatcagaag attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta
     1861 tccaacagtt caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca
     1921 tgatcaatcg ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt
     1981 tcctcattat tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt
     2041 ttttgcgtca ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt
     2101 aacgtttcca taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact
     2161 ttaggaagtc gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta
     2221 agaaaactcc actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa
     2281 tttggttttt aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct
     2341 gtttgtttca aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc
     2401 caattaaatc caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg
     2461 aatttttcat catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa
     2521 gcagcttttg gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat
     2581 tctctttgtt catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg
     2641 cctagcgcgg tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac
     2701 aacatattca gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc
     2761 cagcctactc gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg
     2821 aacaaatcag gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga
     2881 tcatagattt cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct
     2941 tctaataatt cttttggata aagtgcccca gtaggattat taggattaat gataacaata
     3001 gcttttgtcc gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc
     3061 tcttcatcgc aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac
     3121 aacggatagt ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata
     3181 cacatcgtga tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc
     3241 ggaaaatgtt tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg
     3301 gaatctgagt agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt
     3361 ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc
     3421 tcttcttgca tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct
     3481 tctaatttgt tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag
     3541 ttagaacttt ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa
     3601 ttttgccact tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac
     3661 ttaattgtca tgatagtcat tccatagatt cgctgcccaa tccattgcaa ttccacaaat
     3721 gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa
     3781 gatcattccc agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat
     3841 ttttgagctt gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat
     3901 gtgttggaat tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt
     3961 cgagtcccat catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt
     4021 ttgaacgttt ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac
     4081 aatcatgtaa acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac
     4141 ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc
     4201 gagggatttt ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc
     4261 acggttaatc caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct
     4321 ttaactgcca ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt
     4381 ccggaagccc atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc
     4441 atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca
     4501 attaaacata gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa
     4561 agtgacagtt tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc
     4621 attgcccttt tatttccatt gacgtaagta aagctttgac ccattgtatt accaaaataa
     4681 ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac gcacataata gttttgacat
     4741 ttcctatatg ttagaaggaa aattaagaaa aatagcaata aaaataagat attcatgtct
     4801 aaaaa
//
LOCUS       DAYYGY010000128         4795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000128 DAYYGY010000000
VERSION     DAYYGY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.132"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACHA52_002756"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACHA52_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8941402.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="ACHA52_002757"
     CDS             complement(2065..3063)
                     /locus_tag="ACHA52_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8941403.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="ACHA52_002758"
     CDS             complement(3289..3942)
                     /locus_tag="ACHA52_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8941404.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACHA52_002759"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACHA52_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8941405.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1406 a         1007 c          744 g         1638 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttt
//
LOCUS       DAYYGY010000129         4707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000129 DAYYGY010000000
VERSION     DAYYGY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.133"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..353
                     /locus_tag="ACHA52_002760"
                     /pseudo
     CDS             102..353
                     /locus_tag="ACHA52_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            446..1240
                     /locus_tag="ACHA52_002761"
                     /note="aph(3')-IIIa"
     CDS             446..1240
                     /locus_tag="ACHA52_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8941406.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1650..1733
                     /locus_tag="ACHA52_002762"
                     /pseudo
     CDS             1650..1733
                     /locus_tag="ACHA52_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1782..1865
                     /locus_tag="ACHA52_002763"
     CDS             1782..1865
                     /locus_tag="ACHA52_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM8941407.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1990..2727
                     /gene="erm(B)"
                     /locus_tag="ACHA52_002764"
     CDS             1990..2727
                     /gene="erm(B)"
                     /locus_tag="ACHA52_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8941408.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2990..3853)
                     /locus_tag="ACHA52_002765"
     CDS             complement(2990..3853)
                     /locus_tag="ACHA52_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8941409.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3855..4127)
                     /locus_tag="ACHA52_002766"
     CDS             complement(3855..4127)
                     /locus_tag="ACHA52_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8941410.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4144..4359)
                     /locus_tag="ACHA52_002767"
     CDS             complement(4144..4359)
                     /locus_tag="ACHA52_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8941411.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4451..>4707)
                     /locus_tag="ACHA52_002768"
     CDS             complement(4451..>4707)
                     /locus_tag="ACHA52_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFM8941412.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          795 c          865 g         1601 t
ORIGIN      
        1 tgggagtttt gagactacta tatgcgtttt tttgttgatt taccaacatg tttagacatt
       61 ttcctctagg aaaaaattaa ctagcaccac gggttatctt ttatatagaa gatatcgccg
      121 tatgtaagga tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat
      181 gggcaaagca taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag
      241 cttgtaaatt ctatcataat tgtggtttca aaatcggctc cgtcgatact atgttatacg
      301 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
      361 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
      421 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
      481 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
      541 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
      601 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
      661 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
      721 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
      781 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
      841 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
      901 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
      961 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
     1021 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
     1081 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
     1141 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     1201 gaaaataaaa tattatattt tactggatga attgttttag tacctagatt tagatgtcta
     1261 aaaagcttta actacaagct ttttagacat ctaatctttt ctgaagtaca tccgcaactg
     1321 tccatactct gatgttttat atcttttcta aaagttcgct agataggggt cccgagcgcc
     1381 tacgaggaat ttgtatcgat aagaaataga tttaaaaatt tcgctgttat tttgtacatt
     1441 taacttgacg gtgacatctc tctattgtga gttattagtg gtacagtttt caaccgtttt
     1501 aattataaaa aagtggtgca tttttaaatt ggcacaaaca ggtaacggtt attgcaggtg
     1561 tatttcttat ctatgggttt aacatggatt ttatcattaa aatcatgagt attgtccgag
     1621 agtgattggt cttgcgtatg gttaacccta aagttatgga aataagactt agaagcaaac
     1681 ttaagagtgt gttgatagtg cattatctta aaattttgta taataggaat tgaagttaaa
     1741 ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc
     1801 gttatgtaga taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata
     1861 aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa
     1921 ctaaaattac aaacaaatcg tttaacttct gtatttgttt atagatgtaa tcacttcagg
     1981 agtgataaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
     2041 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
     2101 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
     2161 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc
     2221 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
     2281 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
     2341 cgtgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
     2401 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
     2461 ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc
     2521 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
     2581 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
     2641 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
     2701 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt
     2761 acacgttact aaagggaatg tagataaatt attaggtata gggcacctct aataactacc
     2821 aattaattca cctcaaaatg aaattagaaa aaaacagcga aatttttaaa tctatttctt
     2881 atcgatacaa attcctcgta ggcgctcggg accccaacat gaggaaatgt ttttataaaa
     2941 aaagagtagt taccgttttt tggtaactac tcttttcctt ttttaaggtt taaatacctg
     3001 gaagtttagg tgttttgggt attggtggtg taggtggctg caagctttcc aatttttgtt
     3061 gaattgcttt aaattcaggt gtctcttggt gtttatttag aaccattttt tgctctattc
     3121 gttctaaagt cggttgaatt tctttccctg atactttacg attcaattct ttttctaagg
     3181 tttcttttgg actaatagaa ggtgtttcca aacttgaata gagttttact ccttctctgt
     3241 tatatagcct tatatcgcta aataagcccg ttttatgaag ggtttctaaa ttggtcggta
     3301 agtttttgac aacaatatca tgcgcttgtt ttggtgttgc cctggctgtc attggatcat
     3361 ctgcatacat ggtttcatat cgttcaattg ttcctaaata tgagttgatt ttaggtactg
     3421 ccatgacata catttttgtt tcataacctt tggcttgaag cattgttgcg gtttgaatag
     3481 gaacgtctgt tgttcgtcct gtaccttcga tcaccaaatt atacccttga tcgctcaaac
     3541 ggcttatgat cgcttctgtc atgcgattag aataaggggt aacgtgtttt actacgtctt
     3601 tttcataaag tttcactagt tcatcaaaat tagggtgctg ttgtttaaag gtatcattat
     3661 caatgacaat aacattccct tgtgtttctt caaaaattgc tgatcgcaaa ctggttttcc
     3721 ctgaccctgg ttgcccacca agtaaaaaag cggttggcga ttcaaccgct ttttttcctt
     3781 gaatcaattc ttctaaatta tcatttaagc gattctcaaa ttgtttgtca gtaaaattga
     3841 ctatatttgc catattaagc cactttctct ttattcaaaa cttgttttga gtaacgattc
     3901 attgaccgcc aatactcatg aacggcttgt aattcttcta aagaataatc aaaaagattt
     3961 acacgttttg caagctttaa ttggtttaat aaattgactt ccaataattg agagtcattt
     4021 ttatttaatt catggttcaa aacatagttt aatactcgat tataaacgct tgccgataat
     4081 tcgttaatga tctctatttc aaatgttttt tcatacgtaa ctgccatttc attttcactc
     4141 cttttaaagt ttgtcaggta aatatttccg tatatattct tctaaggctt gatccataac
     4201 ttcttttacg tttccgccat tctttgctgt ttcgattttt atgatatggt gcaagtcagc
     4261 acgaacacga accgtcttat ctcccattat atcttttttt gcactgattg gtgtatcatt
     4321 tcgttttgct ttttgtgcgc ctaaatttcc cacaatcact cacttctttc tatttcttct
     4381 tactcttatt ttatcatcaa caatcacaaa tcacaagtga tttgtgatta atcacttgtg
     4441 atttgtgatt ctattctttt tcgttttcta attgaataat tcgctcaagc atttcaaaaa
     4501 atacgttctt atacatggat aaaacttttt tgtcatagcc tttatgctct gtaatgccgt
     4561 ttttagacca ggtacttact ttattacttc gcttgataat attttggaaa accaagttat
     4621 cttctttatg ttgcttatac agttcttcca ggtttgattt tatcgttgcg ctgtccgtat
     4681 caactaaata aggaacaaaa ccgatca
//
LOCUS       DAYYGY010000130         4667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000130 DAYYGY010000000
VERSION     DAYYGY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.135"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACHA52_002769"
     CDS             complement(52..522)
                     /locus_tag="ACHA52_002769"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8941413.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACHA52_002770"
     CDS             complement(681..1139)
                     /locus_tag="ACHA52_002770"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8941414.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACHA52_002771"
     CDS             complement(1310..2455)
                     /locus_tag="ACHA52_002771"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8941415.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACHA52_002772"
     CDS             complement(2468..3385)
                     /locus_tag="ACHA52_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8941416.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACHA52_002773"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACHA52_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8941417.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4513..4647
                     /locus_tag="ACHA52_002774"
     CDS             4513..4647
                     /locus_tag="ACHA52_002774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941418.1"
                     /translation="MKDEWQKLEKEKTALIYEEIEMIHKIENIRTIPNYIEAKIFFHH
                     "
BASE COUNT         1462 a          848 c          880 g         1477 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttt
//
LOCUS       DAYYGY010000131         4620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000131 DAYYGY010000000
VERSION     DAYYGY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.136"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..959
                     /locus_tag="ACHA52_002775"
     CDS             153..959
                     /locus_tag="ACHA52_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8941419.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            977..1684
                     /locus_tag="ACHA52_002776"
     CDS             977..1684
                     /locus_tag="ACHA52_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8941420.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1681..2652
                     /locus_tag="ACHA52_002777"
     CDS             1681..2652
                     /locus_tag="ACHA52_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8941421.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2645..3673
                     /gene="vanB"
                     /locus_tag="ACHA52_002778"
     CDS             2645..3673
                     /gene="vanB"
                     /locus_tag="ACHA52_002778"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8941422.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3679..4287
                     /locus_tag="ACHA52_002779"
     CDS             3679..4287
                     /locus_tag="ACHA52_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8941423.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4353..4526)
                     /locus_tag="ACHA52_002780"
     CDS             complement(4353..4526)
                     /locus_tag="ACHA52_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941424.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1300 a          972 c         1230 g         1118 t
ORIGIN      
        1 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
       61 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
      121 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
      181 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
      241 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
      301 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
      361 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
      421 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
      481 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
      541 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
      601 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
      661 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
      721 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
      781 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
      841 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
      901 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
      961 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
     1021 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
     1081 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
     1141 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
     1201 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
     1261 ctggttgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
     1321 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
     1381 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
     1441 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
     1501 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
     1561 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
     1621 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
     1681 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
     1741 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
     1801 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
     1861 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
     1921 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
     1981 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
     2041 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
     2101 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
     2161 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
     2221 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
     2281 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
     2341 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
     2401 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
     2461 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
     2521 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
     2581 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
     2641 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
     2701 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
     2761 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
     2821 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
     2881 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
     2941 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
     3001 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
     3061 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
     3121 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
     3181 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
     3241 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
     3301 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
     3361 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
     3421 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
     3481 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
     3541 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
     3601 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
     3661 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
     3721 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
     3781 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
     3841 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
     3901 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
     3961 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
     4021 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
     4081 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
     4141 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
     4201 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
     4261 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
     4321 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
     4381 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
     4441 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
     4501 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
     4561 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DAYYGY010000132         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000132 DAYYGY010000000
VERSION     DAYYGY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.137"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHA52_002781"
     CDS             186..2855
                     /locus_tag="ACHA52_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8941425.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3494)
                     /locus_tag="ACHA52_002782"
     CDS             complement(2946..3494)
                     /locus_tag="ACHA52_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8941426.1"
                     /translation="MSRVSSDINTITSRIGLTKAMHMVLKYNQEDPPVPKTYEAIDLI
                     IVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIEKRLSRYPQLYSRIGFAHEFDNL
                     SKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIKITKGNFRLIHRLFAQIDRILRI
                     NQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHA52_002783"
     CDS             3680..4219
                     /locus_tag="ACHA52_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941427.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHA52_002784"
     CDS             4216..4554
                     /locus_tag="ACHA52_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8941428.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAYYGY010000133         4484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000133 DAYYGY010000000
VERSION     DAYYGY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.138"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(593..2098)
                     /locus_tag="ACHA52_002785"
     CDS             complement(593..2098)
                     /locus_tag="ACHA52_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8941429.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2095..2781)
                     /locus_tag="ACHA52_002786"
     CDS             complement(2095..2781)
                     /locus_tag="ACHA52_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8941430.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2974..4395)
                     /gene="gndA"
                     /locus_tag="ACHA52_002787"
     CDS             complement(2974..4395)
                     /gene="gndA"
                     /locus_tag="ACHA52_002787"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8941431.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1250 a          930 c          737 g         1567 t
ORIGIN      
        1 cgccctacac atttggtttg tcttactttg ttcagttttc aaaggtctaa tctttttgtt
       61 ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc tttgtttatt
      121 ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca tctttattgt
      181 tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat tagctatctc
      241 atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact attttttata
      301 atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc aatctcttta
      361 taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg aatattacag
      421 cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg gatgttattc
      481 tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac ttgcttgtat
      541 tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg ctttacttgt
      601 cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct actacacttt
      661 ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac aaccccagac
      721 catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga ttgaagatct
      781 tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc tcaaattcat
      841 tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac tttactgcgt
      901 tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc agggtcactt
      961 cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc ttgaaattat
     1021 tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa tgaacatcta
     1081 cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata cggctgattt
     1141 cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc ccccaacgat
     1201 ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca tgagatacgt
     1261 cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc atctcattga
     1321 atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg tggatatctg
     1381 attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt ggttttaaga
     1441 aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca acttctaaaa
     1501 caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat tcatagaagc
     1561 tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg atagataaaa
     1621 agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt
     1681 ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa acataaagat
     1741 ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag tttccttcga
     1801 ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc agattacgat
     1861 acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct acttctgaaa
     1921 tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat gatttatagg
     1981 taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca aaggcccact
     2041 tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc tttatcacga
     2101 gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc cgggaacgtc
     2161 aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa cttctgtttc
     2221 gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca cgttttccat
     2281 taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat ttccgcgacg
     2341 tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag tttgtttagc
     2401 cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac gtgctaataa
     2461 ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa gtccagatac
     2521 acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt tgacttggcg
     2581 tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca acaatatggc
     2641 atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt ggacttctgc
     2701 gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct tttcatcttc
     2761 aatgattaga atattgctca tttattattt tatcctttct attctactgg ctttttaaaa
     2821 actctatcat tataccgata atatgcttaa aaatctatag tcattttatc atggatagca
     2881 attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt cgtcctcttt
     2941 ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc atgagtaatg
     3001 gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc
     3061 ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag cgatggctga
     3121 tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt ctctgactga
     3181 ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat tttcgatctc
     3241 aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc
     3301 agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc
     3361 gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat acaacgcttc
     3421 tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga
     3481 caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc gggcaaatac
     3541 agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc ttgtccattt
     3601 acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt tacctgttcc
     3661 ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc tgtctaattc
     3721 gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt ttaaaatatt
     3781 cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa taccgttgtg
     3841 taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac atggtgctcc
     3901 gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat aggcttcttt
     3961 ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac cggaaacgcc
     4021 tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca tcgtatcttt
     4081 aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag gaagtaactc
     4141 ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc gacgtggttt
     4201 ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt tatctgggtg
     4261 ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca ctgaatagcc
     4321 gcggctttct atgttcaagg ctagattttt acccataacg gccataccga caacgccgat
     4381 ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat ctcttacatt
     4441 atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAYYGY010000134         4467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000134 DAYYGY010000000
VERSION     DAYYGY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.139"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1429
                     /locus_tag="ACHA52_002788"
     CDS             95..1429
                     /locus_tag="ACHA52_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8941432.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1470..2381
                     /locus_tag="ACHA52_002789"
     CDS             1470..2381
                     /locus_tag="ACHA52_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8941433.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2378..3232
                     /locus_tag="ACHA52_002790"
     CDS             2378..3232
                     /locus_tag="ACHA52_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8941434.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3229..3846
                     /locus_tag="ACHA52_002791"
     CDS             3229..3846
                     /locus_tag="ACHA52_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8941435.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3848..4366
                     /locus_tag="ACHA52_002792"
     CDS             3848..4366
                     /locus_tag="ACHA52_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8941436.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1340 a          763 c          902 g         1462 t
ORIGIN      
        1 ttagaatcat accttataaa ttgttatttt cagaacaaaa tgatatcgtt atattaaaac
       61 cggcatcggt tacattaaaa tttggaggta aaatatgaaa aaattattgt ttacagggtt
      121 gctggcaagt tcagcattgt ttttattagg aggatgcgga tcaagtaatg caagcgatgc
      181 cagttcaagt gcagcaaatt cttctgaaaa aacaagtgat ggaagaaaaa atattgattt
      241 ctggtccttt tggggatccg gtgcacgaaa agaagctatc gaagaaatca ttgaggattt
      301 caatcaatca caaaacgaga tccatgtaaa ttataagtat cagccatggg gtgacatctg
      361 gactaaatct ttatctgcaa taacagctgg aaatgcaccc gatgttattg tacaggacat
      421 aaattccgtt gcccaaagag cagaagcaca gcaagcaact aatctctcca aatatgtaga
      481 aaaagacact tctaaggatt tttatccgca gctttgggat acagtagaat atgaaggtga
      541 tccttatgcg atcccattca atacagatac acaagtcatt ttttataata aaaagttatt
      601 taaagaagct ggaatcagtg aacaagatct accgacaact tgggatgaat tagaagaaac
      661 agcacgtaaa ctagataaaa aagaaggaaa agaatttaca cgcatcggtt tttatccatt
      721 atggaattta ggggctgatg tatgggcgct aaacgcagat aacgggacta gttggtttga
      781 caaagatgaa aaagtcaaga ttgatactaa aaataaagtg gaagccttgg agtggatcca
      841 agactggcaa gattattatg ggaaaaatac gatcaataaa ttagaagccg aatttgggtc
      901 tggtgtatct gatccattta tttccggatt agtagccatg cgtgcccaaa atatcaatta
      961 ttactctagt ttgatggaaa atgcaccgga tgattttgat tttggcgtga tgcagctccc
     1021 agaaaaagaa gaaggttcag gccattggtc gtggggtggc ggattcgtct tggaagtacc
     1081 atacggagca aaagatcctg aggcctctta tgaattcatc aaatatcttt cttctccaga
     1141 agtacaagaa aaattcggcg aaaaaagttt tgatatcatg gcgagcaaga cagccaatga
     1201 gaatttagtg aataatgaca acctaacgga taaaggaaaa atgatctatc aaatggcgga
     1261 cgaaaacttt aaaaatacgg tgatcacacc agttccttta gatgctccgg attacacgac
     1321 attagtgaat gaacagattg accagattct tttaggaaca aaatcaccaa aagaaggatt
     1381 ggcagatgca caaaaagcag tagaaaactt agtcaagcaa aatcaataat ccatttttga
     1441 ctgtctaggt acagaggagg caagaaaatt tgaagcgttt atctatccga aagaaaaaag
     1501 aagcaagatg ggcctattta tttatctcgc cttttattat cgggttcttg gtatttatgc
     1561 tagggcctat gcttttttca gttattggta gtttcaccga ttataatttg acatccaaga
     1621 tgaatttcat tggtctagca aatttcaaaa gaatgttcgt tcacgatgat ttattttgga
     1681 aatcgttata caacaccgtt tatttcgtca tcttcaatgt accgctgacg acgattttca
     1741 gtgtgttttt agccacagta ctcaatcaaa aaatcaaagg aatcagtgta ttccgtacag
     1801 ctttctattt accggcagtc ttatcaggag tagcagtcta catcttatgg atgcagctgc
     1861 tttctccatc tgcaggattg atcaacacat ttctgggctg gttccatatc cctggaccag
     1921 cttggctgtt tgatccacaa tggacgaaac ctgcgttgat tttgatgaaa atatggagtt
     1981 ctggaggagc aatgctactt tacttagcta cattacaaaa tgttcctaga tctttatatg
     2041 aatcagcaga aatcgatgga gcaggcgttt ggggaaagtt caaaagtatt actttgccat
     2101 taatcactcc tattattttt tacgatgtga tcacctctac gatcggttct ttccaaattt
     2161 ttcaagaagc ttatgtcatg acgaaaaatg gaacaggcgg acctgcaaat tctttgcttt
     2221 tttataatct gcatatgtgg aacaaggcgt tcgtagcttt tgatatgggt tatgcgatgg
     2281 ctatgtcaat gatcctgttt ctgatcgtgt tggttctgac gtttgtcaat ttgaagcttg
     2341 cacctaagtg ggtctattat tctggaggtg acggcaaatg atggattatt tcaaaaagac
     2401 cagtgtgaat cggcaaacaa aaaaaatact aagttatgtc ctcatgacag taataggaat
     2461 cattttgatc attccattgc tttggatggt gtttacttca ttgaaaccaa tggaagaaat
     2521 cgttcgttat cctccgacct ttttcccaga aaaaatcgta tgggaaaact atctggatac
     2581 gattgccgcc tttccttttt ggcgctatgc aagaaataca ttattcatca ctgtgctagt
     2641 agtgatcggt aatgtccttt caaattcttt tattgcttat ggatttgcga agcttgattt
     2701 tcctgggaaa aagctgatgt ttgctttggt tctttctacg atgatgatcc cggggtttgt
     2761 aacgatgatc cctcaatatg tattattctc caagatcggc tgggtaggta cttatttgcc
     2821 cctgatcgtt ccatcgttct tcggcaatgc gttcaatata tttctgatgc gtcaatttta
     2881 cttatcgatc aataatgaat tgatcgaagc tgctgaaatc gacggagcga atcatttgta
     2941 tatttggagt catttgatgc ttccattgac gaaacctgcc ttgattacca ttgcgatcaa
     3001 ttccttcaac gcagcttgga atgatttctt aggtcctctg ttatatatcc aagatcaaga
     3061 aaaatacacg ttacagattg gcttgcaagt ttttcagaac caagcaacga cacaatggaa
     3121 ttatctgatg gcaggagcaa cacttgtctt gatcccgacg attttgctgt tcttttttgc
     3181 acaacgttat ttcattgaag ggatggattt gacaggaggt tcaaaaggat gatgaatcga
     3241 tcgattggta aaaaagccaa tgattatttc ttagaaatca ttgaatggat tccacgtctt
     3301 atccatttac agctactatg gttcctgtgc gtcttgcctg ttttcacatt aggaactgca
     3361 agtcgaacag ttctgtatat gatttatcat tacaaaaaaa atgaagagaa aaagctcggt
     3421 tcactattct ggaaaaagtt tcgtgagaac ttctctcttt atggaaagca agatagttta
     3481 gcaagtgttt attttcttct tctattgatc gattatcaga ttttccgcta ttggggtggg
     3541 ggaattggct atacattgat gtatactacg ttgtttttgc ttctattaag cgcgcttctc
     3601 tttagttaca aaatcttgct tcaactggaa caaaaagaag cttcttggat tactgcattt
     3661 ttcttgtttt ttaatgattt caaaagcgcg cttttctatt tggcaggcac gttgatcctt
     3721 cttgtcgcat tctggtttgc aggaccaatc tattttgctt tattaggttt cagtttcttg
     3781 ttttattttc aagtcctgct ttttatccat cgtacgcaag aaaaggttct taaaaaggag
     3841 gaataacatg ttgcgtttga ttgctaaaat cgatgatagt attatcaaca agttcgccaa
     3901 gcaacggaaa acggatagtc gaaaaatcaa ttttttgaaa tttatcagta atatctctaa
     3961 tggtggtttt atttggttgc tccttgctgc tgttttagtc ttgatacctg atactagaga
     4021 atatggtcta gccattctta tcggcagttt gatctatttt tttgttggtt tgctttttat
     4081 caaaaaaacg acaagacgga aaagacctta tgaaaaaaat cgtcggcttg tcctttacac
     4141 aagtaaaccg aagacttctt cttttccttc aggacataca gcgctagcct ttttatcagt
     4201 agctgcgttg ttcgaattaa gtccagttgc aggaatatta gcctttttat ttgcttgttt
     4261 gatcgcttat tcgaggattt ttttactttt acattatcct actgacattt taggaggagc
     4321 tattttagga agtgttattg gcatcctatg cgctgccgtt atctgaatta attgtacgaa
     4381 taaagcatga gtgcgtaaat gcgcatttgt gctttatttt tttgtttcga ggaaagcgta
     4441 gttgtttatt catttgtctt aataact
//
LOCUS       DAYYGY010000135         4461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000135 DAYYGY010000000
VERSION     DAYYGY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.140"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACHA52_002793"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACHA52_002793"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8941437.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACHA52_002794"
     CDS             2298..3221
                     /locus_tag="ACHA52_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8941438.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACHA52_002795"
     CDS             3237..3776
                     /locus_tag="ACHA52_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8941439.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          668 c          757 g         1369 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc gtctctatcc attggtagag
      421 gggctacttg gcatagtgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAYYGY010000136         4453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000136 DAYYGY010000000
VERSION     DAYYGY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.141"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1670)
                     /locus_tag="ACHA52_002796"
     CDS             complement(150..1670)
                     /locus_tag="ACHA52_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8941440.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1913..2098)
                     /gene="tnpB"
                     /locus_tag="ACHA52_002797"
     CDS             complement(1913..2098)
                     /gene="tnpB"
                     /locus_tag="ACHA52_002797"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8941441.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2082..2264)
                     /locus_tag="ACHA52_002798"
     CDS             complement(2082..2264)
                     /locus_tag="ACHA52_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941442.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2505..2723
                     /locus_tag="ACHA52_002799"
                     /pseudo
     CDS             2505..2723
                     /locus_tag="ACHA52_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3218..3817
                     /locus_tag="ACHA52_002800"
     CDS             3218..3817
                     /locus_tag="ACHA52_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8941443.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3881..>4453
                     /locus_tag="ACHA52_002801"
     CDS             3881..>4453
                     /locus_tag="ACHA52_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFM8941444.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         1421 a          803 c          792 g         1437 t
ORIGIN      
        1 attattcagc aaatatgaga atggtttttt tatttgatta gacataactt aataattcag
       61 tagttttgag cgtaagcgtg tcatagaatg gtacctaaac acaaatagcc cgatttgata
      121 cactcaaatc aggttatttg tggcttatat tatttacaga tttcttgtat ctgctgattc
      181 catggcaaat aagcgcgtag cgcttcaggt gtcatcgaag acagattggg aatctctgta
      241 aaaagatgat ttaaatattt gcggatgttt aacccattcg caacagctgt ctggataata
      301 ctcaatataa ttccatttga tctagcgcct tttaagcttt tagaaaatag ccagtttttt
      361 cgaccgatca ctaactcttt gatggcacgc tctgctcggt tatttgaaag ttccaagcgt
      421 ccatcaagca acacaatctg aagtccgtct ttatgattga ctgcgtattg aaccgccttt
      481 ccaagctgcg attttggcaa ataagcaaaa ctctcacacc aatgaaaaaa ttcagtcatc
      541 acaggctgca acgtttcctt cctcgctaca aacctttctt ctgcgctaag cttattaaag
      601 gtccgttcga ttcgaaacat cttgtcacag aagtctaatc ctattttggc atgacttttt
      661 ggggacggtt gtttacccaa tgcttcaaaa aactttctgc gaacatgcgc ccaacaataa
      721 acattgttta ccggcagctt tccataggcc gcgtaaccat cagtatgcaa gaaaccgcta
      781 aatgattcta aaaaactttt tggctcatca acggaacgcg aatttgcgtg ctcgtagagc
      841 acaacttgtt tttcttcgct tttacctgaa gcatataacc agtaaaaggt cttttcttta
      901 tgagtattca atacccgata gcttgtctca tccgcgtgta agacctcctg attgcttaat
      961 tcttgatgaa ggcaattgta gattggctct aaaaccattt cacttacctt gatatgccaa
     1021 ttagtgattt ttttacgatc aagagggaaa ccaagtgcat gccattcttt ttcttgtcga
     1081 tatgccggca cctttttaac aaatttttcc caaatactct gtgtcacaat tgaagaggaa
     1141 cctagactat tattgatcaa cggtttagga acaggcgctt ttttgatttt tgtttctcct
     1201 aatgcttcac attctctgca atgataggca tgttggtaga tgacttgttt ttctatatgg
     1261 gcaggaacat acaatggctc ttctctaagc cttgtcgcac cgatatcatg taaagtatga
     1321 ccacagtctt cacacaaaca atcggcacct tttaattcat aatgaatagg aacttcaggc
     1381 agaccttcta atgaaagtgc tttgagtccc ttgcgttttc tgcgtttaaa agaagatacc
     1441 gtaatgggtt cctggctttc ttgtccagtg tgctctggtt cattaaaagt tgagtcgtct
     1501 aataaatcga tttgcccatc tacgggtggt gtttcttttt ttcgtccaaa caagcgtcga
     1561 ttcatttcag caatttgttc tctaagaaga gtattttcct gttgctggat ttcaagcaac
     1621 tgtgttaatt gcttgatttg tacaagaagt tcagattcca tttttgtcat ttttacacca
     1681 ccattctagc gattttctta catctattat accattttat gacaacgaaa aacagacaaa
     1741 atccttgatt taacaaggtt ttcgtctaat atatcgcacc ttttttcgtt tcttttagcg
     1801 tacatttttc ttcgattgaa aggccagtca gtaagacttc ttttctggaa cgcggccatt
     1861 gaaggtaccc actttcaaat cttttgtaca ttaatacaaa tccattttga tcctagtaaa
     1921 gagccttgaa gcgatctttc ttcgtaccgc aaaataagaa aagcgcttga tcgtaaacat
     1981 caaggtcata ttcttctata acgaaagctg ctagtccatc aattcctttt cgaagatctg
     2041 tttttccaca aacgataaaa atatgtttca atgcagttgc atcaatgagc attggcattc
     2101 aactctttta acaaggtata caaaagatat ttatccattc cattataaat aaatatttcc
     2161 ttatcttccg atttgattct gcaagctagt tttggcttgg tgtcaatttg cgatttttga
     2221 gccgcacttg aaaattgaac tggtacaata tcttttttat tcattcgttt ttctccttct
     2281 atatttgata gtttaattat agaagaaaga taatcggcgt acgagatact atcctatgac
     2341 acgcttacat ttaatttaat attttgcctc aaattagcca cactttttat agaactaaga
     2401 ggaaaaatga agttttcttc ctattaatat agttgtttga aatcttaaat atagcgtttt
     2461 acgatgtaat gggaagtatg gtgaataaat caactatgtt cgtacgaagc atttactcaa
     2521 ctaatttaat cgaatctttc aataaacaaa tcaagaaata cagccaccgc aaggaacagt
     2581 tccaaaatga agagtcgatg gaacgtttct tagtctcgtc ttttgatact tacaaccaaa
     2641 aattcctagg tcgcagtcat aaaggtttcc aacaggccga aggcgagctc gaacaaatgc
     2701 tgagtcaacc gatggagaat tagaaacaat ctacagtagg gaagaaccat tgacacaaaa
     2761 ttattgacgc tcccaactaa aagtgtcaaa ataatgatga aatcatttag ttcttataaa
     2821 ttagaaaaga gaaaataaat attccatatc tactttattg gatccgtaac atgactaaat
     2881 tctgaatact ttttaaagct tatcatttgg taaaactgaa tataagttaa ttatatagac
     2941 tgaaaatttt gaattttcag aatagaataa cttaaaagta ctgacaaaat tttttaaagg
     3001 ggcaatatta atgttctgag ataatcaaat cctaccgaaa atatcaaaaa tccaatgtga
     3061 cacaatcaga ttgtgtctca ttcgagggaa tcacacaatc ccacgtggct ctaggcagaa
     3121 ttgaattttt gcttggggct ttttccttat attgattttg aatgagactt attatatgtt
     3181 ataatgcaat taagtatcat tggagagggc gaagaaaatg aagacggtag aacctattcg
     3241 agacaaaaaa aagatcgatg cgatgaaggc aatcttggct tcgggaaaat atggccaacg
     3301 aaatttggtt cttttttcga ttgggatcaa caccgcctat cgcatttctg atttgcgtca
     3361 attgaagctg gccgacgtgc tggaaatttc acgcggtcgc gtggtggtca aagatcgatt
     3421 agcgatgaag gaacaaaaaa cagctaaaca taattcggtc tatatttcca acaaattgcg
     3481 aaaaacgatt ttggagtacg ttcaacagga atttcctgat caactccttc agcaagattt
     3541 tgacggttat ttgttcccta gtcgaaaagg gacgaatgaa ccgctcaacc gccaaagtct
     3601 gtggcgaatc attcatgatg ctggcacggc aatcggattg aaagacattg gcccacattc
     3661 catgcgaaaa acgttcggtt actttctcta taaacagggg accaaaacag aaatcattca
     3721 gtccttgttg aaccattctt ctcagcgcga aacattgcgg tatattggta ttacacaaga
     3781 agacaaagat acagctgtaa aaagcttaga tttataatat aaaagttaag tttaaacatg
     3841 gatagtttta aattagtaga ttgagtgttg ggaggaaaga atgagtacat tatatattat
     3901 aggaaacggt tttgatttaa gtcatggttt aaaaactaga tacactgatt tttcaaaatg
     3961 ggttgaaaaa agtcatagtg aaatattcga taaaatagac acaatgataa atgaaaataa
     4021 tgaggtgaga agtacagctg atgataagat aggttggttt aattttgaaa gtagtttgca
     4081 tgagttaatt caaagtccag tctttgaaga ttatgtagaa gagtctaagg attatctcat
     4141 aggttatgga catgaagatt ggtcagattc tgcccatcat gattttcaaa atatggttta
     4201 tgattatgtt gtttctctag aagaattaca acaacttttc aaggaatgga tacgcaaaga
     4261 aatacagccg acaatagaac ataaaattcc aaaataccaa ttttcgtccg atgtactatt
     4321 ctttacattc aattatacag atgttttgga aattctatac aagcaaccga aagaaaacat
     4381 atatcatatt catggcagtt atgacgatat aataatcggc cactccgaga attttgacaa
     4441 ctttatagga gat
//
LOCUS       DAYYGY010000137         4375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000137 DAYYGY010000000
VERSION     DAYYGY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.142"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..1051)
                     /locus_tag="ACHA52_002802"
     CDS             complement(77..1051)
                     /locus_tag="ACHA52_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8941445.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVQESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2906)
                     /locus_tag="ACHA52_002803"
     CDS             complement(1500..2906)
                     /locus_tag="ACHA52_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFM8941446.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3127..3489)
                     /locus_tag="ACHA52_002804"
     CDS             complement(3127..3489)
                     /locus_tag="ACHA52_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941447.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3493..3801)
                     /locus_tag="ACHA52_002805"
     CDS             complement(3493..3801)
                     /locus_tag="ACHA52_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941448.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3810..4229)
                     /locus_tag="ACHA52_002806"
     CDS             complement(3810..4229)
                     /locus_tag="ACHA52_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941449.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
BASE COUNT         1234 a          968 c          631 g         1542 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatccaag ctttcttgaa
      361 ccattgcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaagtttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaggtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc acatcgtaac
     1501 taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt ttgacgataa
     1561 aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat atccttttct
     1621 gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt ttttaatcgc
     1681 tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc cacttgtaaa
     1741 tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc tacatactca
     1801 tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt atcagcattt
     1861 ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc attcccagat
     1921 aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaaccat atctttttgg
     1981 aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc ttcaatcttc
     2041 actctctcag aagccaccag agccttttct cgctcatttt cttctctttg cctagaaata
     2101 tacctcaact catcagaaac gctctcacgc tcttttacgg ccgtttgacg caattctgaa
     2161 atctcgctca tgatttcttt atattcgtga acatctttga ttttgttagt accgactaac
     2221 tcacgctccc aaccaaaatc tgccatcaaa cgttcaattg actcaacttc ctgattccta
     2281 aaattattcc aaatcactcg accattctct ttatctgctc gtaaaccctg ctgttgcaag
     2341 gctttgttaa aacttggttg cttttgaaca ccccgtttat acccttcagc aacaggaaca
     2401 atattcaaat gcaggtgcgg acttacttcg tcattatgaa taacagcatt gtagacggat
     2461 aaattaggat tccgttcttt aaattctttg aaatattcaa ctaaaatttt gttggcaatc
     2521 actcgatttt gttctgcttg ttgttccgat atgccagtag aagaaccatc atcacgagta
     2581 gtacgaaaat catcaagagt accaatttgc acgataaatt ctcgctgtgg ttccaatttt
     2641 ttatccttgc gaacctttgc taaatagtcc tcgatttttc tatcggctct tttctgcttt
     2701 gcattatatt tttctacggc ttcaccgaat aattcttcat atttttcacg aatatttgtt
     2761 tgttctaaat agatattttc atccgatttt gtgaaatcaa tatgcttatg ccagtcattg
     2821 attttttggc tatcagttaa ctcacgatta ttatgagaaa tgcttgtatt ctttgttccc
     2881 tttttgaacg aaatactgaa ccccaagtta aaaccctcct tcggctgtcg ctaataaaaa
     2941 gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat tgtgacatgg
     3001 ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag
     3061 gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat
     3121 aatcgcttat cgtaaattca cgtcattaaa tattgacgta aatttactct cactcatctg
     3181 acttttctta ttcggtatca ctccgaataa gccgtcagat tcatcgggat aatctcgttc
     3241 gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt cgtgttgctg
     3301 atcgtcagaa atcgacgata gccaccgctc aaaatcagca aaagtttcaa acccaaaacg
     3361 cttccgaaga gccttgccca ttgcagcatt ttctttctca atcaagcgtt ttttctgttg
     3421 gcgaagtttc gcttgttctt tttttaattt ttcttcttgc gctttaatat ctgctaaagt
     3481 cattcccata aactacatct ccttttcttt ggaattaata acggaaattt ggtttaactg
     3541 cataacatga ctcattaatt tcaaccaaaa ttctttttct gattcatcag ttgtttgcgc
     3601 taaatgctta tgaacaaatt cattagtcaa aactgaagat tttgctaaat cagcattgat
     3661 aaccgagttg agaacatcca taattcgttc aagcatttca ctataattat cttcatccgg
     3721 caaattcaaa taatcagcta accgttcata catcaattca attctatcga ctgtttcttc
     3781 tctcaaatca attttatcca tgtcacacct cacttccaga attgccacca ttttcgattt
     3841 tttaattctt gattttctag taacaactga tcgttttttt gttgctgaag cagggtcaat
     3901 ttttgttgat cacgaatcat ttctgttaat tcagaaatat gtttgtcctt ctcagctaat
     3961 tgatcacgca aaaactcatt ctctttttct aaaggaaaat cactgcctgt gttattgacc
     4021 ggtaattgac cagtcaattg atcacttttt gaccgataaa ttgaccgaac aacaccaatc
     4081 aactctttat ctagcatgat aatgccattt tctttatact gagcaaggtc aaaccctgaa
     4141 ttgaccagtt gattgcgcac tgtatttttc gatacaccca attcgtcagc aagatttttt
     4201 atggtcatct tttctgacaa atcagtcatt agcttctcca ctactttcag ctaccgcata
     4261 aatatattta acggcgtgct tagggataat aatcggagag tcaattttat tatttttcaa
     4321 aatcaattca tattgcgtta catcaagtaa cttaccggac atttttttgc tatcc
//
LOCUS       DAYYGY010000138         4335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000138 DAYYGY010000000
VERSION     DAYYGY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.143"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..296
                     /locus_tag="ACHA52_002807"
     CDS             63..296
                     /locus_tag="ACHA52_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941450.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="ACHA52_002808"
     CDS             complement(455..577)
                     /locus_tag="ACHA52_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM8941451.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            732..991
                     /locus_tag="ACHA52_002809"
                     /pseudo
     CDS             732..991
                     /locus_tag="ACHA52_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1434..1640
                     /locus_tag="ACHA52_002810"
     CDS             1434..1640
                     /locus_tag="ACHA52_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8941452.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="ACHA52_002811"
     CDS             1640..1891
                     /locus_tag="ACHA52_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941453.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            1907..2344
                     /locus_tag="ACHA52_002812"
                     /pseudo
     CDS             1907..2344
                     /locus_tag="ACHA52_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2455..2691)
                     /locus_tag="ACHA52_002813"
     CDS             complement(2455..2691)
                     /locus_tag="ACHA52_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941454.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIILFKWITF
                     FLLSYRALPVYFAPPKRRGRLERLVRNKNHFLSY"
     gene            2789..2968
                     /locus_tag="ACHA52_002814"
     CDS             2789..2968
                     /locus_tag="ACHA52_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941455.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3027..3383
                     /locus_tag="ACHA52_002815"
     CDS             3027..3383
                     /locus_tag="ACHA52_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941456.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1528 a          675 c          836 g         1296 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     3181 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     3241 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     3301 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     3361 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     3421 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     3481 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     3541 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     3601 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     3661 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     3721 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     3781 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     3841 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     3901 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     3961 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     4021 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     4081 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     4141 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     4201 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     4261 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     4321 taaaggtttt taata
//
LOCUS       DAYYGY010000139         4304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000139 DAYYGY010000000
VERSION     DAYYGY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.144"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1136
                     /locus_tag="ACHA52_002816"
     CDS             147..1136
                     /locus_tag="ACHA52_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFM8941457.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1133..1552
                     /locus_tag="ACHA52_002817"
     CDS             1133..1552
                     /locus_tag="ACHA52_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941458.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1561..1869
                     /locus_tag="ACHA52_002818"
     CDS             1561..1869
                     /locus_tag="ACHA52_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941459.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1873..2235
                     /locus_tag="ACHA52_002819"
     CDS             1873..2235
                     /locus_tag="ACHA52_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941460.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2457..3863
                     /locus_tag="ACHA52_002820"
     CDS             2457..3863
                     /locus_tag="ACHA52_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFM8941461.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1496 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaga ttgctttttc ttccctagta ttttatagtt ttaattgact agaaaaaaac
       61 taaactagaa aggagagcaa tctatttttt gttatcggct tttcagcctt ttttgtatac
      121 aaaatttgta gattcagtat agcaagatga aaaagaaaaa gcaagatttg attaacggaa
      181 catttcgaga aaaaaagaaa aatttgaaaa aagtggtggc atttagtgat aatcgaattt
      241 ctcccaaaat gcgtgaattg atgacaggct gtggttcata tttagaattt attgcaacga
      301 ttgataagga gaaaaagaaa ttggtccaag ctcacttttg taaaaatcgt ttctgtccgt
      361 tatgtgcttg gcgtaaagct cgtaaggatt cgatgatgtt gtcgattatg atgcaagcta
      421 ttgcggaaga aaagcagtat caatttattt ttatgacgtt gacgacaccg aatgtaaaag
      481 cggaatgttt gaacgatgaa attgatttgt ttaatcatgc attgaaaaag atgtttatgc
      541 gcaaacgggt taagacttcg attaaaggct atgtgcgaaa attggaaatc acgtacaaca
      601 aaaaacggaa tgactataac ccgcattttc acttgatttt ggcagtaaat aaatcttatt
      661 tcacggataa agattattat atcaagcaat cggaatggtt agatttgtgg cgggaagtga
      721 ccggaaaaac gggaattaat ccggatggta ccgacgaaat tacccagttg gatattcgca
      781 aggtcaaagg gttccaacaa gaaaaagcag tacaggaagt tgcgaaatat tcggcaaaag
      841 attttgaact gaccgaaagt caggaagtgt ttgacacatt ctatcatgca atgaaaggtc
      901 gtcagttgat tacgtttaac ggtgtattca aagaatacaa gaaaaaatat gagtcgggtg
      961 accttgatcg ttataagaaa aaagatgaga atgaatattt ttggtttttg actgcttcgt
     1021 ggatgaggaa agaatcgaaa tatgcggttg attatcgtga gatgagtgct gatgaaagaa
     1081 attatttttc aggaatgtat gtcgatgaaa aatctgttga gggggaaaat gaatgactga
     1141 ttcaacggat aaaatgacaa ttaaggcgct tgctgatgaa ctaggaataa ccaaaaatac
     1201 agtgagaaat tatctagtca aagccggata tgatcttgct cggtacaaga aaaatggaat
     1261 tatcttgcta gacaaagagt tgattggtgt tgctcggtca atttatcggt caaaaggtgg
     1321 tcaattgact ggtcatttac cggtcaatag cacgggcagt gattttcctt tggaaaaaga
     1381 gaatgagttt ttgcgtgatc aattagctga gaaggacaaa catatttctg aattaacaga
     1441 aatgattcgt gatcaacaaa aattgaccct gcttcagcaa caaaaaaacg atcagttgtt
     1501 actagaaaat caagaattaa aaaatcgaaa atggtggcaa ttctggaagt gagatgtgac
     1561 atggataaaa ttgatttgag agaagaaaca gtcaatagaa ttgaagtgat gtatgaacgg
     1621 ttagctgatt atttgaatct ttcggatgaa gataattata gtgagatgct tgaacgaatg
     1681 atggatgttc tcaactcggt tatcaatgct gatctagcaa aatcttcagt tttgactaat
     1741 gaatttgttc ataagcattt agcgcaaaca actgatgaag ctgaaaaaga attttggttg
     1801 aaattaatga gtcatattat gcagttaaac caaatttccg ttattaattc caaagaaaag
     1861 gggatgtagt ttatgggaat gactttagca gatattaaag tgcaagaaga aaaattaaaa
     1921 aaagaacagg cgaaacttcg ccaacagaag aaacgattga ttgagaaaga aaatgctgca
     1981 atgggcaagg ctcttcgcaa gcgttttggg tttgaatctt ttgctgattt tgagcggtgg
     2041 ctatcgtcga tttctgacga tcagcaacac gaaaaatcag tgggtaatga gttgcgagct
     2101 gatgaaaaat cagcaccaag cgaacggaat taccctaacg aatctgacgg aatattcgga
     2161 gggataccga atagaaaaag tcagatgagt gagagtggat ttgcgtcaat gtttagtgac
     2221 gtaaattcac gataagcgaa tattttgcat gaagcaaaat aaagcgtaaa aggagttgag
     2281 agcgtgacac gctttcggct ccttttttcg ttgtcacttg tgacggcgaa aaccctcgga
     2341 gagcgtttgg aattgtgaca gtcatgtcac aattaggaaa acgttactct ttgacaaaga
     2401 gtaacagaaa ggtttacact ctttttatta gcgacagcgc gaaggagggg tttaacttgg
     2461 ggtttagtat ttcgttcaaa aagggaacaa agaatacaag catttctcat aataatcgtg
     2521 agttaactga tagccaaaaa atcaatgact ggcataagca tattgatttc acaaaatcgg
     2581 atgaaaatat ctatttggaa caaataaata ttcgtgaaaa atatgaagaa ttattcggtg
     2641 aagccgtaga aaaatataat gcaaagcaga aaagagccga tagaaaaatt gaggactatt
     2701 tagcaaaggt tcgcaaggat aaaaaaatgg aaccacagcg agaatttatc gtgcaaattg
     2761 gtactcttga tgattttcgt actactcgtg atgatggttc ttctactggc atatcggaac
     2821 aacaagcaga acaaaatcga gtgattgcca acaaaatttt agttgagtat ttcaaagaat
     2881 ttaaagaacg gaatcctagt ttatccgtct acaatgctgt tattcataat gacgaagtaa
     2941 gtccgcacct gcatttgaat attgttcctg ttgctgaagg gtataaacgg ggtgttcaaa
     3001 agcaaccaag ttttaacaaa gccttgcaac agcagggttt acgagcagat aaagagaatg
     3061 gtcgagtgat ttggaataat tttaggaatc aggaagttga gtcaattgaa cgtttgatgg
     3121 cagattttgg ttgggagcgt gagttagtcg gtactaacaa aatcaaagat attcatgaat
     3181 acaaagaaat catgagcgaa atttccgaat tgcgtcaaac ggccgtaaaa gagcgtgaga
     3241 gcgtttctga tgagttgagg tatatttgta ggcaaagaga agaaaacgag cgtgagaagg
     3301 ctctggtggc ttctgagaga gcgaagattg aagaagagaa gaaaaatatg ctgaatgtaa
     3361 aaaaagagat ggcggtcatt ttccaaaaag atatggttcc tttagaaagt ttcaagaggc
     3421 ttcctgatgg ttcgtatcat ttatctggga atgattttgt tgatttgtat caacgagcta
     3481 cgagagcaac aaatgccaag agaaatgctg ataacatgag tgagcaatat ttacagatgc
     3541 ttgagcaaaa tacagaatta aatgagtatg tagctgcatt ggaagaagag cgtcatttgt
     3601 ctggtagaaa acaattagca gatttacaag tggaaaataa aaagctgaaa gaagaaaatc
     3661 gctctttacg ccagcgttta gagcgattaa aaaacgcatt tcaaaaatcg atcactcgtt
     3721 tgtctatccg ttttgggctt gcagaaaagg atatggggct ttctgaggaa ttagaagtat
     3781 tagagcagga gaaaacctta ttttatcgtc aaaacgtctc acagcataaa ttagagcgtt
     3841 ctgaagatga gatcgagtgg tagttacgat gtgcagtcaa cggtaccgga ggcggtttgt
     3901 gccgaggata aggagttgac ggctaaacca ctagcctatt aagctgacat ttgctaaaaa
     3961 atggcaagtc cattttttgg ccaacttagc gttagcgaag tttgggggcg ccctttttaa
     4021 agggtgcggt tttatgtttg catgactaag aatgagtggg aaaatttaac agccctatct
     4081 aacaggcggt taaatgtaca aaatatgtac acccccttaa aaatgcagtg gtcgattgat
     4141 cgaattttat gtctttgggt tttggggttt taaaacgaaa aaaagggggc gattttcgcc
     4201 ctcttttttg atgttttttc ttatcttgat actataggca actatttctg cgaagaaatc
     4261 ggggctatag tatgggtcta tcccttgtgg ctctaaggaa gaac
//
LOCUS       DAYYGY010000140         4295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000140 DAYYGY010000000
VERSION     DAYYGY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.145"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..790
                     /locus_tag="ACHA52_002821"
     CDS             <1..790
                     /locus_tag="ACHA52_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8941462.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="ACHA52_002822"
     CDS             887..3136
                     /locus_tag="ACHA52_002822"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8941463.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..>4295
                     /locus_tag="ACHA52_002823"
     CDS             3139..>4295
                     /locus_tag="ACHA52_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8941464.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1501 a          610 c          791 g         1393 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact agagt
//
LOCUS       DAYYGY010000141         4288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000141 DAYYGY010000000
VERSION     DAYYGY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.146"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..793
                     /locus_tag="ACHA52_002824"
     CDS             92..793
                     /locus_tag="ACHA52_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8941465.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1365..2381)
                     /gene="tsaD"
                     /locus_tag="ACHA52_002825"
     CDS             complement(1365..2381)
                     /gene="tsaD"
                     /locus_tag="ACHA52_002825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8941466.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2378..2842)
                     /gene="rimI"
                     /locus_tag="ACHA52_002826"
     CDS             complement(2378..2842)
                     /gene="rimI"
                     /locus_tag="ACHA52_002826"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8941467.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2849..3391)
                     /gene="rimI"
                     /locus_tag="ACHA52_002827"
     CDS             complement(2849..3391)
                     /gene="rimI"
                     /locus_tag="ACHA52_002827"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8941468.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3375..4097)
                     /gene="tsaB"
                     /locus_tag="ACHA52_002828"
     CDS             complement(3375..4097)
                     /gene="tsaB"
                     /locus_tag="ACHA52_002828"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8941469.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1265 a          916 c          740 g         1367 t
ORIGIN      
        1 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
       61 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
      121 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
      181 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
      241 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
      301 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
      361 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
      421 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
      481 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
      541 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
      601 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
      661 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
      721 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
      781 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
      841 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
      901 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
      961 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
     1021 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
     1081 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
     1141 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
     1201 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
     1261 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
     1321 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
     1381 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
     1441 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
     1501 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
     1561 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
     1621 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
     1681 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
     1741 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
     1801 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
     1861 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
     1921 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
     1981 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
     2041 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
     2101 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
     2161 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
     2221 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
     2281 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
     2341 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
     2401 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
     2461 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
     2521 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
     2581 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
     2641 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
     2701 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
     2761 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
     2821 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
     2881 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
     2941 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
     3001 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
     3061 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
     3121 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
     3181 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
     3241 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
     3301 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
     3361 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
     3421 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
     3481 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
     3541 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
     3601 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
     3661 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
     3721 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
     3781 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
     3841 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
     3901 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
     3961 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
     4021 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
     4081 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
     4141 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
     4201 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
     4261 ttcatttccc cacagctttt ccatcttt
//
LOCUS       DAYYGY010000142         4065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000142 DAYYGY010000000
VERSION     DAYYGY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.147"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..1430
                     /locus_tag="ACHA52_002829"
     CDS             81..1430
                     /locus_tag="ACHA52_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8941470.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1558..2148)
                     /locus_tag="ACHA52_002830"
     CDS             complement(1558..2148)
                     /locus_tag="ACHA52_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8941471.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2295..3647
                     /gene="brnQ"
                     /locus_tag="ACHA52_002831"
     CDS             2295..3647
                     /gene="brnQ"
                     /locus_tag="ACHA52_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8941472.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          745 c          789 g         1359 t
ORIGIN      
        1 tttggaatct tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa ttaaactttt
       61 ttagttagga tgatttaaaa atgaatgaag agcaagaact gcagcgtgga ctgaaaaata
      121 gacatgtcca actgatttct attggcggag cgattggaac ggggttgttt ttaggttccg
      181 gaaagtctat ccagctagct ggcccttcga tattgctcgc ctatcttatt actggatgca
      241 tctgtttttt cattatgcgt gcgttaggcg aacttctgtt atcaaacacc aataaccatt
      301 cttttctgga ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc actggctgga
      361 cgtattggtt ctgctgggta tcgattgcaa tggccgattt aacagccatc ggaatgtatg
      421 tacgctactg ggcgcctggt gttcctcagt ggcttcctga actgattgca ttaggtcttc
      481 ttctttgctt gaatatggtg gctgtctcat tgtttggtga gttagagttt tggtttgcat
      541 tgatcaaagt tgtagcgatc attgccttta tcctcgtagg agcttatatg atcctcaccc
      601 actatacgac agatattggc acagccagta tcacgaatct atggtctcat ggcggattct
      661 ttccaactgg tgcaaaagga tttatcttag ctttccaaat ggtaaccttt gcttttgctg
      721 ggatagaatt agttggatta gtcgctggag aaacagaaaa tcctgaaaaa gtgttgccgg
      781 aagctatcaa taatattcct atcaggatca tcttatttta cttaggatct ctttttgtca
      841 ttatggcaat ctatccatgg aacagcttga atccagataa cagtccattt gtagaagtat
      901 tttcggaaat tgggatcacg attgctgctt ccttgatcaa tttagtcgta ttaagtgcag
      961 cagcttctgc ctgcaacagt gcaatatata gtacaggacg tatgcttcgt tctttagcgc
     1021 aagaaggcag tgcccctaaa aaattcaaaa agttaacaac acatcatgtg ccagggaatg
     1081 ctttgacatt ttctactatc gtcattttca tttctgttat cttgaattac gtgatgccta
     1141 gtgaagtatt cacactagta agcagtattg ccactacttg tttcttgttt atctggagta
     1201 tgcttgtcta cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa gctcattcat
     1261 ttaaaatgcc gttgtaccca tttagtaatt atctggtatt tctttacatg gcctttgtgt
     1321 gtgtcgtctt gttcctagga aaagatacga gaatcgcttt attactaaca cctgtgtggt
     1381 tcttgcttct attactaatc tatcatatga aatatgaacg taagaagtaa gaaagtagtt
     1441 gtgacagaag tggtcagctt cgagaaataa gaaggaattt acgaaaattg cttcttaaat
     1501 ttttgtgaaa tttcggctta tttcttggag ataaaagctt ctgtcccagc ctcttttcta
     1561 tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa aaatccaaat
     1621 accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga caccggcaat
     1681 caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca aaactctcac
     1741 gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac caaaagaaaa
     1801 actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt cagggttacg
     1861 cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa agatattcga
     1921 ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat ggttcaccca
     1981 gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc ggatgaccca
     2041 taaagaatga aagctagatg tattagggat cttgatctct aatacaagaa ctctcaaaac
     2101 aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga tgattcctcc
     2161 tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccat cgttttatat
     2221 agggaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt aaaaaaaccg
     2281 taaaggagtt atgcatgaca aaaaaattat catttaaaga ctatcttttt ataggctcca
     2341 tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat ttgggtcaag
     2401 aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt atcggtcttc
     2461 ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat gaattagcaa
     2521 aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta gttatcggac
     2581 cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta gcaccattta
     2641 tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt tttgtgctgg
     2701 cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag ttcctgaatc
     2761 ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat ccactaggtt
     2821 ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc acagggttta
     2881 ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc atcattgtca
     2941 caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac acgatcaaat
     3001 ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct tatttaggtg
     3061 ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg gctcagattg
     3121 ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt atattagctt
     3181 gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca gatttgtttc
     3241 cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt ttagcggcaa
     3301 acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc atctatccat
     3361 tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag cgtacggctg
     3421 tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg ttgaacgctt
     3481 gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt gcggaaagct
     3541 acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc ggttttgtta
     3601 tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa aagaggttgt
     3661 gaaagagacg ttcagccttg agcaataaaa agtaattttg aaaaatggct tctcacattt
     3721 ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgttta ttgagaggtc
     3781 gtgaaaaagc cgcttagttt caagtattaa gaaatatttt gtgaaaatgg cttctcacat
     3841 ttttgcataa atttgactta ataccgagaa actggctttt gaacaccgtt tattgagcgt
     3901 ctataaactg acctgagtat tagagaaaca aaacggcaaa gtagctccat atactttgcc
     3961 gttttgtttt gaactaatac cgaagagaag ctgatcaaac aacatttctt ttataatata
     4021 gattttagac atatatatga tataaaaata gttttcgcaa aaaaa
//
LOCUS       DAYYGY010000143         4034 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000143 DAYYGY010000000
VERSION     DAYYGY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.148"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACHA52_002832"
     CDS             73..234
                     /locus_tag="ACHA52_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8941473.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACHA52_002833"
     CDS             253..1530
                     /locus_tag="ACHA52_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8941474.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACHA52_002834"
     CDS             1540..2196
                     /locus_tag="ACHA52_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8941475.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACHA52_002835"
     CDS             2196..3572
                     /locus_tag="ACHA52_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8941476.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="ACHA52_002836"
     CDS             3702..>4034
                     /locus_tag="ACHA52_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8941477.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          705 g         1277 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgga gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DAYYGY010000144         4003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000144 DAYYGY010000000
VERSION     DAYYGY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.149"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            749..1789
                     /locus_tag="ACHA52_002837"
     CDS             749..1789
                     /locus_tag="ACHA52_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8941478.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPSLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2613..2945
                     /gene="traE"
                     /locus_tag="ACHA52_002838"
     CDS             2613..2945
                     /gene="traE"
                     /locus_tag="ACHA52_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8941479.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3564..3905
                     /locus_tag="ACHA52_002839"
     CDS             3564..3905
                     /locus_tag="ACHA52_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8941480.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1462 a          510 c          720 g         1311 t
ORIGIN      
        1 ttttatgact tgtacaaatt tatctgatat tctcttatta agaactacct aatttgggta
       61 gttcttattt tttacatttt aagtagtatt taattgcaat ttggtagtat ttttttatta
      121 tcttattctt agaacggtag tacttaatat aaatataggt agtatctaat ttaagtatta
      181 atggtagtat ctaaattaac aggtagtatg taactacttt aaagtaattt ttttcagatt
      241 aacacagtag tatcttatta aataagttaa ttttaatggt agtacataat tattatatat
      301 atctgacatt ggtagtactt attgtatttt ttagtagtat ataatctatt gttttctagc
      361 ctaaagcctt gtcttataag aataatataa tatatactac tgaaataagg tagtatatat
      421 tactagtaat ggtagtatgt tttatatgca taaggtagtt cttaatttat tttttgtttt
      481 gacactggta gtacttaatt atctttaatt ttaaaactaa attttattca tacgtttttc
      541 tggtagtatt taattataaa taatatttat ttaaagtagt tcttaatgac tttaaaggta
      601 gtacataatt cattttattt ctgattttag gtagtattta attaaaaaac agttttaatg
      661 cgtttgcatt ttttatttat ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat
      721 aaaacaacgt gaaagtagag gatctacaat gactgatttt aaatttttta aggcggatag
      781 agtttacaac gaattatttt atcaatttcc aaaagtcttt attgtttctg acgaatacaa
      841 aaaaatgaaa gattcaacta agattgccta tatgctttta aaagcaagat tagagatcgc
      901 aatcagcaaa cggcaaatcg atgaagaagg taatgtttat tttacttata cgacaaatga
      961 actatgtaga gtattaaact gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga
     1021 atcctttggt ttattattac aaaagcagat gggatttaac aaacagttag ggaaaaataa
     1081 tcctaataga ctatatctag cagaattaaa agtctcagaa aatgatatct acttactcga
     1141 aaaatttgat agagagaata gggaaaacgt tgataaatca gagggtatga aaatcatacc
     1201 ctccctcgac gaaaaatcag acgctgaatc ccttggggct caagagggta tgaaaatcat
     1261 accgtgccaa aacgttgata aatcagaggg tatgaaaatc ataccagaac ttaataataa
     1321 tatattagac actaatagac acaatataga cactgaaaaa gaccgcctac aagatcaatt
     1381 gttgttagac aattttgaga caattatgac aaacgacagc attgctacgt ttgtccctga
     1441 acgagtatta aatttgataa aaacattttc ttcaagttac agtgaagctc aaaaaaccgt
     1501 ccagactatt cataatgcaa agaaaaaagc tgaaatagaa agtgatattt cgatagtttt
     1561 tgaagaactc gatagttatt atgtcaatgc agaacaagaa ttatacacga cactgttaaa
     1621 agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta
     1681 tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt
     1741 gaattatgca agctccccaa gcactattac gaaagactgg ttagagtgaa actttactga
     1801 caaagtgatt gttgcttcat ggagtaacat taaaattaaa aattttttat tattctaatt
     1861 atattacgaa tattacaaaa taatagctat tttaatagaa tttataagtt aaaatattat
     1921 gataaaaatc acaaacgtag ccttttacgt cataagtgtt aaaagaaata attaatttca
     1981 aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata ataatacgcg
     2041 aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt cttttcaggc
     2101 aatacgaata ttattcgctt acattttttg tataaattta attgtctgct atgcttgtga
     2161 tgaattgatt ttcgttttgc acttttggat gataaatttg gacaattgaa aatacaaaaa
     2221 aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca tacacggcat
     2281 taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg caatggattt
     2341 agtataacag atattcaaaa gtgataccac tttttttggc tgtgttaagg ttaaaaatac
     2401 gtgatattac gatgaatcta tgcctaagca taatgttata aaaatgtccg tgtggctctt
     2461 tgccgtatga cgaacgctga aattagaggg aacgcatgtt tggatgcgtt ccctcttttt
     2521 tgtttgtcat gaacgaaaat caattctcta tttttgaaaa ataacaagaa aaacttgtta
     2581 aaaatattta aagaaattgg gtggtttatt ttatgtcata tacaggaatt ttgagtctca
     2641 aagatatttg tcactacggt aaacggtgta ccgctacaga gaaaatcacg aagaaactat
     2701 caactggcca aagtaaaacc gttgtgcagt gtaagaaata tataatccag aaagacaaag
     2761 tctcagagga aatgatttat tacattggga aacaaaagca aataatttta aaagatccta
     2821 tccccttaaa agagctatat ccaacaatca aacatgttta tgaccaaaac ggtgtactga
     2881 ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg
     2941 gctgaattga tcattctcat aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg
     3001 tcgagacttc taaaggaact tagagtcact agaaaaataa ggagtagact tgatctatca
     3061 acaattccct tcggggagtg acggggtagg ttttaacgat tggtaacgta aaaaattggt
     3121 caatcgagta aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa ataataagcg
     3181 cagagtaaac aagagggaat ttagaagtta gacaagttag aatagcgtct agattaatca
     3241 tgagcagcta ctaagggatc tagactttga cgcaaactca tggaataagg agtaggtctt
     3301 ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat gtagatactt
     3361 gcgaggacgt tgattattgg atcaatcgag catttaccgc tgtctactag ggtttctcgt
     3421 ttcggacgcc ataaacgagt cgaaattttt tatgtatttt taagtaaacg aaaaacgata
     3481 aaatatgaac aagtcctgaa taaagtgaaa aaattaatat aaagcataga gaaaatgaaa
     3541 tttacgtaat ggaggaatga caaatgaaaa attatgatcc gaatatacga ttgggcacac
     3601 atactattaa ggtttctttc caacgttggg attacaaagg atttgtgacg tttagaaggg
     3661 gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta tacgatcaaa
     3721 cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa ggtaatgaat
     3781 ggtttaaaat gacgcttatg aatgataatg gagacgaatt atctgttgaa gatacttggt
     3841 catatttgag tgattatatt gtcagctttg aaatcattga atttgtagcg gataaagaag
     3901 aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca cgacgattaa
     3961 taaaaaggta gtattacctt aggcattgag taaggaattt att
//
LOCUS       DAYYGY010000145         3995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000145 DAYYGY010000000
VERSION     DAYYGY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.150"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..664
                     /locus_tag="ACHA52_002840"
     CDS             <1..664
                     /locus_tag="ACHA52_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8941481.1"
                     /translation="PIDCSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFR
                     RTIHERNSLYPHLTKEFGHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDI
                     ENRLNDWFKSMPKHLFKSMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHS
                     NGLLRRDGLPKTMDFRDTDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSS
                     LI"
     gene            781..1089
                     /locus_tag="ACHA52_002841"
     CDS             781..1089
                     /locus_tag="ACHA52_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8941482.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            1116..1415
                     /locus_tag="ACHA52_002842"
     CDS             1116..1415
                     /locus_tag="ACHA52_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8941483.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            1589..2140
                     /locus_tag="ACHA52_002843"
     CDS             1589..2140
                     /locus_tag="ACHA52_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8941484.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            2286..2579
                     /locus_tag="ACHA52_002844"
     CDS             2286..2579
                     /locus_tag="ACHA52_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8941485.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            2572..2868
                     /locus_tag="ACHA52_002845"
     CDS             2572..2868
                     /locus_tag="ACHA52_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941486.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2939..3943
                     /locus_tag="ACHA52_002846"
     CDS             2939..3943
                     /locus_tag="ACHA52_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8941487.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         1484 a          590 c          787 g         1134 t
ORIGIN      
        1 tccaattgat tgttctgttc gtacactcta tcggatgttt aaaaaaggcc tttttgacac
       61 acagtcgtta cctatgaagg ggaaacgtaa accgaataat catcaagaaa agcgtggaaa
      121 acaggccttt cgcagaacga ttcatgaacg gaacagtctc tatccacacc tgactaaaga
      181 atttggtcat ctcgaagtcg acaccattgt gggagttaaa cacaagagcg ctgttattac
      241 attggttgag tgtttatcca aggttattat tactttgaaa ccgacaggca gacgagccga
      301 agatatcgaa aacaggttaa atgattggtt caaatccatg cctaaacact tgtttaaatc
      361 catgacattt gattgtggaa aagaattttc caactggcga tctataagta actcgaacga
      421 tattgccatc tttttcgcag atccaggaac accttcgcaa agagggctaa atgagcactc
      481 aaacggcttg cttagacgag atggcttacc taaaacaatg gactttaggg acacggatga
      541 aacctttatt caatccgttg cctcgaaaag aaaccatatt ccaagaaaat cattaaatta
      601 tcgaacacca ttggaagtat ttttgagctg catagataaa gacatattgt ctagcttaat
      661 ttgacaaatc agatagtata aaatgaatca aattgaatat caaatatata ttatatccat
      721 gaagcccaat cttctttaat agaagattgg gctattttta tggcaaagga gggaaaagaa
      781 atgtttcaaa ataaaggaca acgttatgtc acaaagggag taatggacag tttgccggtg
      841 gaattacaag ctttatgttg gaatctaatc gatcaaaacg ttcaaaagca atttcattta
      901 gactatcttc aaatctttga attttctacg gaaaaaggaa atcaaaagtt ggttcatcgt
      961 caagaagaac cagaagcaag aaaagagtat ctgatttcac ctaagcttcg tttgaaatca
     1021 gtgtctcaaa aaatatgggt cattgattcg ggtgagtatc aaacgatgtt attaccagaa
     1081 gaatactgat atctaaaaga aaggaacgaa aggacttgtt agaaacggct atcgaagtac
     1141 tggaaaagtg tgcacagcta gtcactactc ctgaagagtg gggctatgaa tccgttacga
     1201 tggaaaaaga ggaaattgaa atgggtatgc ttcctgaaga tgttactcta ccacgtttag
     1261 tgatgacaca tctttatatt tattgcgcac cagaagatgg aaaagactat gtagtttatt
     1321 tcattacaga tattaccagc cagcgagaat ttgttcgtgg gttactggta gaaggacgat
     1381 tggtttggag tcagattgga ggaacgaatg aataagatcg attatttgag aatgattgcg
     1441 aaatgtttag aagatatcct tacagcgatt catcaaattc tgaagaaaaa aggagaaaga
     1501 atatgaaata ttttcaacta gtattgacac ttataacggc aatggtgatt gaattgcttg
     1561 aacaaaaatt agcataggag aaaaagagat gattgaaaaa gaattagtga ccacacaaac
     1621 agaagtttgg cgagatcctc atcataccca tcgctatgtt ttcaaacgac aatgggcgat
     1681 caaaggaaaa gaagcggaag ataaattagc ggtagtcatt actattcgtc caacagatac
     1741 agaaccattt acgaatgatt tgagtatgtt attaatcgaa aagaatatcc gacaattagg
     1801 atttactggt tttattgctg tgaatctttt ttcgtataca aaagcgaaaa gtcctcgggt
     1861 atttcctcgt ggaaatgatg aacaatcttt ggaaatatta acaactgttt tcacagaaaa
     1921 gaaaatcaac accattattt ttgcttgtgg ttcgattaca gcgacgagtc aagtggctta
     1981 tgaacaagcg aagaccattt acgatcaatt gagtgcgaaa caaaaaaaga tgaccaaagt
     2041 attgataaat gcagaaggta agttatccca cccactaagt attcatgttc gtaatgaatg
     2101 gcaattggca gatagtcaat tattatttca agaaaaataa cattttattt tcttatgtta
     2161 cttataagta gttttacatg ctttttgact ttaaaaatac atgtgatgat acatgttata
     2221 tgaattatca atgtacccag taatatgcta ttttcattat gtaaaaaata acagggggtg
     2281 tgtagatgga gattgatgaa acttgtttga gtaactctta taaaagtatg aatgactttt
     2341 ttgaggaaat ggaactggat tcagaattgc tctatcaatt ttatcttgct tttcgagggc
     2401 aacagatttc ttttccaatg aaaatgtacg atcgtgagtt agttagaaaa agaatcgaaa
     2461 acatgataga acaggaaaaa actgtagata tccgtcaatt gacagaagtt tatggtttct
     2521 ctgcaagatg gattcaaaaa gtaattaaac aaaaagaaag gagacgagta catggctaag
     2581 ctgattgtat taggtggttt aatttacggt ggatattggt tatataaata tatgcttgcg
     2641 catccgaata tagataaagg aaaagtaatt aagacagttg gggtaatcat agttggactc
     2701 atagctctaa ctaagaaaaa taatactaat agcccaaaaa taatgagaac ttatcgctgt
     2761 agaggatgtg gaagggaaat ttctactagt tttaatccat ctgaaagccc tcaaggaaga
     2821 gatcgttgtc atgctggcta tcgacattct tgggaaagga ttaactaaat gaacaccatt
     2881 gagattaatt gactgataaa ataagtacgc gagggagaaa ttgaaagggg gaaataaaat
     2941 gaaaaaatgg ttgttattac taattggaat ggggatttta ttcactggtt gtacatctga
     3001 gaatacgagg actaaagaga ctgtagaaac tgatacaaca attgaggcta aaaaaagtaa
     3061 aacgcaatca actgaacaaa ctacaacaga aatgaaacaa tctgtggaag caagtacaag
     3121 tgaaagttca atcattgaat cttcttctga agaggttaaa aatactttgt gggatagtga
     3181 gaaatctact caactagcaa tttttgtccc acaatgggga aagacattag gtcaagaata
     3241 taaaagctac aatcctcaga acaatgtaag tctgtatggt acgcctttac caagtgcagt
     3301 tattgatgga aattggaaaa tggcaatcaa tgaagcacca gttaccgtac aatggtcaga
     3361 agatggaact ggtcatgcag atttcaattt agtagcagtt tattctgatg tagaaactgg
     3421 agaatattta gggcagcatg tatatttctt tggctttcaa aatggacaac ctaaagttta
     3481 tttaacgcaa caaaatcaag gcaatgataa aaactacatg tacttcaatg aaactcagaa
     3541 ccaacagtta aaacaaggtt tcattgatat tgtgaatgga caaactcctc aaactccagt
     3601 agtagaagaa tctactcaac aagcaacaac tacaacgaca caatctacaa gtagctcagc
     3661 aatttcaagt tgggaagctg ctaaagaaac tgtactaaat aataaagctt tatggttgta
     3721 tgattctcct gataaaaatg catcaaatac taatttaatt agcacaaatg aatatatgaa
     3781 agatctaatg caagatgaaa atggaagcta ctattcaatt aatattgtaa cggatgataa
     3841 tggaaataca acaatggctg gtggaaaaca attccgtgtt tatacagatg gaagaatttc
     3901 tcaacgtatt ggaatgacta acaattttca aatgattaaa taattgaaaa tattatttat
     3961 caaagaaaaa ctgattttga gaaaaattat ttaat
//
LOCUS       DAYYGY010000146         3877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000146 DAYYGY010000000
VERSION     DAYYGY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.151"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACHA52_002847"
     CDS             complement(717..3866)
                     /locus_tag="ACHA52_002847"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8941488.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAYYGY010000147         3865 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000147 DAYYGY010000000
VERSION     DAYYGY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.152"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..469)
                     /locus_tag="ACHA52_002848"
     CDS             complement(248..469)
                     /locus_tag="ACHA52_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941489.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="ACHA52_002849"
     CDS             complement(567..812)
                     /locus_tag="ACHA52_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941490.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="ACHA52_002850"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="ACHA52_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="ACHA52_002851"
     CDS             complement(2036..3037)
                     /locus_tag="ACHA52_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8941491.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="ACHA52_002852"
     CDS             complement(3196..3462)
                     /locus_tag="ACHA52_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8941492.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="ACHA52_002853"
     CDS             complement(3452..3808)
                     /locus_tag="ACHA52_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8941493.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1223 a          698 c          511 g         1433 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatatatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cgaat
//
LOCUS       DAYYGY010000148         3844 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000148 DAYYGY010000000
VERSION     DAYYGY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.153"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACHA52_002854"
     CDS             154..417
                     /locus_tag="ACHA52_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8941494.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="ACHA52_002855"
     CDS             417..689
                     /locus_tag="ACHA52_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8941495.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="ACHA52_002856"
     CDS             766..1095
                     /locus_tag="ACHA52_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8941496.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1085..1372
                     /locus_tag="ACHA52_002857"
     CDS             1085..1372
                     /locus_tag="ACHA52_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8941497.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1656..2513
                     /locus_tag="ACHA52_002858"
     CDS             1656..2513
                     /locus_tag="ACHA52_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8941498.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            2514..3062
                     /locus_tag="ACHA52_002859"
     CDS             2514..3062
                     /locus_tag="ACHA52_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941499.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            3322..3617
                     /locus_tag="ACHA52_002860"
                     /pseudo
     CDS             3322..3617
                     /locus_tag="ACHA52_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3692..>3844
                     /locus_tag="ACHA52_002861"
     CDS             3692..>3844
                     /locus_tag="ACHA52_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8941500.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT         1446 a          494 c          780 g         1124 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa acatgtcatt aagtatttaa aaaagctaaa
     1141 agagaagcca ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc
     1201 atataagggt gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata
     1261 tgcgggcaca acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt
     1321 gttatgtgga acgcatgaaa acttttatga acagttgaaa aaattaatct agtgatttgt
     1381 tgtcgttctt aagaaggacg gcttcttttt atcgaaaatc tgattcgtct acatgactaa
     1441 agtcgcgagt attctctgct tctcgataaa ttaaataacg tcattacttt gaggaaagct
     1501 ataaatcaat atatttcagc ttttccttat ttatgtacta ccttttactt attaattata
     1561 aggtagtaca tatatatctt tttttggtag taactaaaaa taaatatgtt ataatagtat
     1621 caaatgaata ttgctaaaag aaaagaggta ggaacgtggt agcgaagaaa tatgtaatcg
     1681 gtaattttaa aggtggcgta ggtaaaagta cgtgcgcgca aatgttaggg tttgaatcag
     1741 cagttaatga aggaaaaaga accttgataa ttgacttgga tatgcaagga aatacttctg
     1801 atgtgatgaa cttaacgcat atgaacttca ctaacgaaga aggaggcgga gaaggtgaac
     1861 cgattgttta cgaaaatact gtgactgacg tgttgataaa caatcttcca cctaaagagg
     1921 ctgtctataa agtaatcaat aacctctata tacttccagc agacatgtcc tttgaaatgt
     1981 atgatgattg gatcaaagac caatataatg attcattatc ccaattcaaa tatatgaaag
     2041 aaaagctaga accattattt gatgagtttg acgtcatcta tttagatgtc ccaccttcta
     2101 tttctgtata tagtaaatcc gcgatgtaca ttgcggattg ggcaatcgtc gtcttacaga
     2161 cccaagtaaa atcgatgaga aatgcgatgc aatatcttga gtatatggac ttttttgtga
     2221 aagaatttga tacaaattta tatgttgctg gtgttattcc tttcatgttg gaaagcggtg
     2281 acgcagtaga tcaggaaatg tataaacaag cccaagagat ttatggggaa catttgatta
     2341 aaaatgttgt actcaaaaat gcgcgcttaa aacgatacga cggatcaggt attacgatgg
     2401 agaagacaaa gaaaggtctc ttaaaacaat gggatcgcaa aagccacgaa ttatttatca
     2461 gtattttgaa agaattggaa gaacacgaga attggtatgc ggaggtggag taattggctg
     2521 acatcaaagg acttgatatt tttgggaaca acaaaggact cgccaaaata aaaaaagaag
     2581 cagaagcaaa gaaaaagcag caagcaatta caccatctga aaataagcaa ttagaagtgc
     2641 accaagttca agaaataaaa acttcctcca atgttgaaag acagagtaga ggaagaacaa
     2701 aaaaagttgg cgcaccaatc agaaagtttg ataaggttta ttccaccaag catcctttaa
     2761 aactgtcgac tttgttaaat gcgacttcca gagttttagt agaaaagtat gagactaata
     2821 tgacaagaga tgaattgctt agaaaagctt tagatgagta tattaaacag aatcttacgc
     2881 aagaagataa gcaagatttg tttaatgacg tggtgaaaga actagacttg ttcagagaaa
     2941 aatatccaat tgttccagaa ttagatgatc gtggagaaat aataagaact accagtcaga
     3001 taaaacaaga taccgaagac tcactgcgca aagggtgggg aatgaataat aaagttagat
     3061 aataatcaaa gaagaactaa gtaactacct ttaaacggta gttacttagt tcttctttga
     3121 tttgcatgaa atagagggga tttatttttc tggttacgtg attaaaaaat ccaaaaaatg
     3181 agcataggaa cggttatgat tttagggtgt gttttaatga gatattgtga tttcattaac
     3241 aagtgtatga taatctgttg ttgagttagt aaataagtaa tagagctaac tctaaaataa
     3301 aactattagg aggagatgta ggtgaaaaag tttaaaaaga aaatatattt aggattggtt
     3361 gttggggtga gtatgtttgg gttcatggct catactaaac aagtaactgc agatactaac
     3421 caaaaagaaa ggattatgag ccaattagat gactctcaaa taagtatgta cccaatgagc
     3481 taattgtcag tttaaaggct acacaaagaa tttcaacaga gaaaaataaa ccgcaaaatt
     3541 tattgggtgt aaaagttatg gtggaaaaag atttgactct taggacaaat gaaagcgatt
     3601 gtaataagcg agatcaaggg ttctgttgca aagttttcca aaaaatctat tttagtgtaa
     3661 aattgagaaa aaagacagag aggacagagt aatgaatcat tttaaaggca aacaattcaa
     3721 aaaagacgtc attattgtcg ctgttggtta ctacctgcgt tacaatctaa gctatcgtga
     3781 agttcaggaa ttgttatatg atcgtggaat aaatgtttgt catactacga tttatcgttg
     3841 ggta
//
LOCUS       DAYYGY010000149         3758 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000149 DAYYGY010000000
VERSION     DAYYGY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.154"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..1951
                     /locus_tag="ACHA52_002862"
     CDS             236..1951
                     /locus_tag="ACHA52_002862"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8941501.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1954..3717
                     /locus_tag="ACHA52_002863"
     CDS             1954..3717
                     /locus_tag="ACHA52_002863"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8941502.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         1126 a          732 c          744 g         1156 t
ORIGIN      
        1 aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga gactggcatg
       61 ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag cttcttcata
      121 ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc ctaagctaca
      181 atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga atcatatgat
      241 tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc cgttcttcaa
      301 atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct tgatcgataa
      361 tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga tggtcgctat
      421 gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag tagcttcaca
      481 aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt tctctcacaa
      541 cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg acatcaatca
      601 gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt ttttaagtat
      661 cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt tcttagcatt
      721 attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc ctctctacca
      781 aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc ttagcggtgt
      841 tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg ataaaagcac
      901 aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat tatcacctgc
      961 tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg ggatcaaagt
     1021 aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata tgaatcaaat
     1081 gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg ccgaagcttc
     1141 aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag ctgaggtcag
     1201 tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg acttccgtta
     1261 tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc gaaatacagt
     1321 ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat tgattccgcg
     1381 tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc gttcttggca
     1441 gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac tatttaccgg
     1501 gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag aatgttggga
     1561 agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg gtttagatac
     1621 aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac tgacgattgc
     1681 aagagctttg attgccaagc cagcattact gattttagat gactctttaa gtgcgttgga
     1741 ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca gtacagtgat
     1801 tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag tgatggatca
     1861 aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag cagaatatca
     1921 ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt atccttgcag
     1981 acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg gctagctgtg
     2041 atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat tggtcaaagt
     2101 atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca tttacttatt
     2161 ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca agtgttaagc
     2221 aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc tcatctgaat
     2281 cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag tcgatttaca
     2341 aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt attttctgga
     2401 gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact tctgacattg
     2461 gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc tcgtgcttca
     2521 caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt tgtgaccgaa
     2581 cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag tcaacagcaa
     2641 tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt ttcttcttcc
     2701 ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat tggtttagtt
     2761 ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat ttccagcttt
     2821 acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat cacgacacaa
     2881 ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca gccagaagaa
     2941 aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg aaaagtctct
     3001 tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga tttcaatttt
     3061 actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg aaaatccaca
     3121 cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac agtcgatgac
     3181 catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat ggtgttacaa
     3241 gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa tccggaagca
     3301 agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt tgtagaacgt
     3361 ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc agatggacaa
     3421 cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt gatcttagat
     3481 gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc cttcttagca
     3541 atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat caaaaatgcg
     3601 gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca tgatgagctg
     3661 cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc acaataaaaa
     3721 caagcagtct tctgggatgg gaagactgct tgtttttt
//
LOCUS       DAYYGY010000150         3696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000150 DAYYGY010000000
VERSION     DAYYGY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.155"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..978)
                     /locus_tag="ACHA52_002864"
     CDS             complement(<1..978)
                     /locus_tag="ACHA52_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8941503.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1002..2486)
                     /gene="galT"
                     /locus_tag="ACHA52_002865"
     CDS             complement(1002..2486)
                     /gene="galT"
                     /locus_tag="ACHA52_002865"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8941504.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2498..3487)
                     /gene="galE"
                     /locus_tag="ACHA52_002866"
     CDS             complement(2498..3487)
                     /gene="galE"
                     /locus_tag="ACHA52_002866"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8941505.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT          980 a          793 c          664 g         1259 t
ORIGIN      
        1 tttcgttgat tctcgaataa tcaactctgt ttccacaata atctttcttg aaaccggtgc
       61 tttggaatta cttaattgaa gaatcgtctc cactgctagc tctcccatcc attcggtata
      121 aacttttaca gtagaaagag agggcgttac atatttcgct acactgacat cattgaaacc
      181 catcacagaa atatcttctg gtaccactaa acctttttct tgaatcgctt tcagtgcgcc
      241 aatcgctaat gcatcattag ctgcaaaaag cgcatcagga acatgcggat tcgtttttaa
      301 atactgcttc actgcttgat atcctgactc gacagaaaaa tctgcttcga taattggacc
      361 ttcgtcgtag cctttttctg ccaaaacttg ttgaaatgcc ataattctgg gatcttttag
      421 ccgctgatgg tttttcttcg tatattctgt tccagcaagc atagcgatat gttgcgcttt
      481 atgggagaca aaatgttcaa tgactttttg aacacttgac ttaaagtcga caactagaga
      541 gtcgattcct tgagaagtgc cgtcagaatc taccaaaagc aaggcatttt gggatccttt
      601 catttctaat aactgagctt cgtcgaattt tcctagtgcg atcgttccat ctgtcggaag
      661 atcactaatg gaatcccacg tttctttcaa taaagtaacg tttgcttctt ctgccttttt
      721 ttcgattcct agacgaatag ataaataata aagatcttca agctcttccg tatcgtcgta
      781 ccactggatc aggcgaagcg tttgcttagt atttgctgca tttttacgat gtttcgtata
      841 atttaagcgt tctgctgctt caaaaatttt ttgctttgtg gctaagccaa cggaaagatc
      901 aggatcatag ttgagaactc ttgaaacagt agcaggagaa actcctgcca actgcgcaat
      961 gtctttaatt gttgccattt gtctctcctc cttttttacg tttataaaat tgcagtaaat
     1021 cgttcaaacg ctgcctgtcc tttttcatct cgcttgaata ctccagcgtc ttccagtaca
     1081 cgtgcaaaaa cctgacctac agatttttgt acgacatcgg tgaccgtatc tttcgtcaaa
     1141 tcaggatgtt ctttttttaa ttgttctgcc cacggacgat gatattcagc catctggttt
     1201 tcttctccta ataagaaatg ctctacttct actagttctg gtttcaaacg aggtggtaaa
     1261 acagcaagcc ccattacttc gatcaaccca atgttctcct ttttgatatg ttggacatct
     1321 ttatgtggat gaaaaatccc atcaggatgt tcttctgaaa cattgttgtc tcgaaggaca
     1381 aggtccaact caaagtaatt gtcccttctt cgtgcaattg gcgtaatcgt atggtgtggt
     1441 gtaccatctg ctgaaaaagc tctgatagat actgtttcat ccgaatatgc tttccacttt
     1501 tccaatactg agttagctgc ttcaatcaac aattttttct ctgttgattg caaacggatc
     1561 acagacatcg gccatttcac gactcctgcg atgacttgcg gataatctgc tagtttgata
     1621 agtttttcta tcggtgcttt tgccatagga aattcatgac gtcctgcctg ataatgatca
     1681 tgagaaagaa tcgatcctcc aacaatcggc aagtcagcat tcgaaccaac aaagtaatga
     1741 ggcaagactt cagtaatccg aagcagtcta gcaaatgttt cctttgtgat tttcatcggg
     1801 cggtgttgtt ctgataaaat gatcgaatgt tcattgtaat aagcataagg agaatattgg
     1861 aatccccagc tttcaccgtc tagattcatt cgaataatcc ggtgattggt gcgggctgga
     1921 tagttcaagc gacctctata tccttcgttt tccatgcaaa gcatgcaggt aggatagtct
     1981 acttttaccg cctcgcgttc tgctgcgatt tgttttggat ctttttctgg cttagataaa
     2041 ttgattgtga tttccagttc accatactcc gactctgccg gaaatttgat gttttttgcg
     2101 atggctcttg ttttgatgta gtcatttttc ttacttaaat taaagaaata ctcagttgct
     2161 tcctctgggc ttttcgaata gtgctgtgca aagaatgcat tgacgactga aggtggcggt
     2221 gtgagaaaat ccattaattg cgcttcaaaa atctcttgtt ctgctggcaa ttctgaaaga
     2281 acaccatttt ctcttgcctt agcgacaagc tgatccaata agtctacaga ttcttcctta
     2341 actggtctta cttcaatgct ttctagagca tcttctccta ccatccctaa aagacgattc
     2401 tgtaaatata agcggtccat ttccatccat ccgccagctt cgatcgcaag tgtggcaaaa
     2461 tcacaaatcg tttgactgat tgacatatct cattccccta ttcttcataa ccatttggat
     2521 ggctgacatg ccaatcccat gctgttttga taattgcttt gatatctgtg tattcaggtt
     2581 tccaacccag aacctctctc gctttttcac ttgaagcgac taatcggcta ggatctccag
     2641 ctcttcttgg tgctatttct gctggaattt cttttcctgt cacttctcta gctgcttcta
     2701 gcatttcctt gactgagtat cctttgctgc tgcctaaatt gaagaagttg ctttcatttc
     2761 cttctttcaa gtattccaaa gctaaaatat gtgcagcaat caaatcttct acttgaacat
     2821 aatctctgat acatgttcca tctggtgtat cataatcgtc tccataaaca gccaatgctt
     2881 ttctttgacc aagagctact tgtaaaatga ttgggacaag atgcgtttct ggtgtatgat
     2941 cttctccgat agatgcatct gcttttgctc cggctacatt gaagtagcgc aaagcgacat
     3001 agcgcatgcc atatgcttgg tcacaccact tcatcatttt ttccatcatc aatttacttt
     3061 caccatacgg atttttagga ttagctggtg tatcttctgt gattggagac tcttttggtt
     3121 ctccgtaagt tgctgcagta gaagagaaca cgattttatt cacattgaac tcgtgcatga
     3181 cttcaagtaa gatttgcata ccgtatacgt tgttgttgaa atacatcaat ggtttttcga
     3241 cagattctcc tactaaagag cttgcagcaa aatggattac tccttcaatc ggttcctttt
     3301 caaatacgga acgtagaaat tctttatccc ggatgtctcc ttcataaaaa tgagcatctg
     3361 gatgtactgc ttgtttgtga cctgtgagca aattatctac cacagctact tgatacccct
     3421 tttgaactag ctggtctaca gcgtgcgaac cgatataacc ggctccgcct agtactaaaa
     3481 ttgtcataaa aaacactcct ttgattagta aaattgtgtt tgttgattat atataattta
     3541 aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat
     3601 ggtaaattta taataaaaca tttagtaaac attttcaagg attttttgca aaggtttaca
     3661 aacaaacgta aaactagcct ttttttctct tttgtt
//
LOCUS       DAYYGY010000151         3615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000151 DAYYGY010000000
VERSION     DAYYGY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.156"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACHA52_002867"
     CDS             complement(<1..461)
                     /locus_tag="ACHA52_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8941506.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQ"
     gene            1098..1184
                     /locus_tag="ACHA52_002868"
     CDS             1098..1184
                     /locus_tag="ACHA52_002868"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8941507.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(1305..1994)
                     /locus_tag="ACHA52_002869"
     CDS             complement(1305..1994)
                     /locus_tag="ACHA52_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941508.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2001..2279)
                     /locus_tag="ACHA52_002870"
     CDS             complement(2001..2279)
                     /locus_tag="ACHA52_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941509.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(2239..3153)
                     /locus_tag="ACHA52_002871"
     CDS             complement(2239..3153)
                     /locus_tag="ACHA52_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8941510.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(3135..3515)
                     /locus_tag="ACHA52_002872"
     CDS             complement(3135..3515)
                     /locus_tag="ACHA52_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8941511.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT          960 a          701 c          657 g         1297 t
ORIGIN      
        1 tattgcttta acagacgaaa catagtcttt gcatagccac tttgtagctc gttgaattgt
       61 tgtaaagaat agcgtaccca ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa
      121 attcgtatat ctacgtaagg attttctacg tccccgttga ttttaaattc tgtaaacatt
      181 acaaacatat cgtaagaaag cccagactca cttctacgac caaagcgtaa agataagatt
      241 ttttcataag ttgcttttaa atcttctaca aatcgtacat ttgcagttgc tttgtagtta
      301 cttaaatctt ttagttggtc aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt
      361 ctagacacga tggaaaaaaa caaattcatt tcaaccggtg taaattttct taaaggaatt
      421 gtatttaatt catggtggta ttttacaatc tcattagcca tataatcacc tcaatccaat
      481 tatgaatgac attttttaaa tgtcaagaca ttcatatttg acgttcaaac agacaaaatg
      541 tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataaacagac
      601 aaaatgtcct tcataacatt ctgtatccat tggtagagta aggacacagc ttacggtaaa
      661 gaggtttaaa aagaggttta aaaagagata taaaaagagg ggggctaatt ttcccgccta
      721 aaaaccactt cttttttctt taaacaggtt cgcgcatttt tcaaaactgc caatccaaaa
      781 ccttgcttcc tacggtcgcc aaaaaggtgt gatcgctacg ctctcacatt gtactgcgca
      841 gtcgcctaaa ggctcctttg ctaaaaacat gttggtaaag aggcctttga gcggttctga
      901 atgcttatct aagccgtttt aagacagtct tggataaaaa tccttatcta gctttaaagc
      961 gccttacagg cggtcagggc aagccctaac aaccctaaaa taagcgatac ttaaaaacac
     1021 cacacaagtt taatttttta agatataata acttgtcgcc tatccgacag tcttgcgact
     1081 ggaaggaggt gtatctaatg tttgaattgt tcatttgccc ccttcttata gaagtttgtg
     1141 tctctttatt cagttattgg ttgaataacc gaaacaagaa gtaaaatcaa taaaggcgac
     1201 atcaacctga aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtatctaatg
     1261 tttgatcatt tgcatgttta gtttaacatg attgccgatc aaattcaatt ttgctgttgc
     1321 tgatctaacc attccttgac actttttaga taatttaatg ccgtttcttt tttcgttgga
     1381 ttttgtatgt tcgttgcttg ctgaagcagt gctttataac gttcgtaggt aactgattgt
     1441 tgccgttctt ttcgttcctg ttcactagtt gaaaaaggga ttgtatcact gaataaatta
     1501 taacaagctt cttctgtctg cgtatcttta aaatattgca cgtctttttt ggttcgctca
     1561 tagttgtctt gaacctcttt ttgctgtttt tctggcaagt tttgataaag acgagtgagt
     1621 ttttccatgt ttttatccgt caaacgtttt tcttgattca caaaaatatc gttcatttcc
     1681 tcataacaag tggtaaactc ttccattttt tgtgttaaca cttgctcctt tttcttttta
     1741 tcacatgctg cttcatactt ttgagtgata tcccctaata aaatacttct tgaaatacgt
     1801 ttatttccct ctaaaacgcc gaataaacca cttaaaacga ttgataatgt aaatataacc
     1861 cctggcaata aaaacctctt aaatcgaatt ttagagcctt ttaatgaata tcttgtttga
     1921 taagccttat agctaagcca aataattcct aaaatactgg caaaaatgag tagatagggt
     1981 aaatagaatg ccatttctat ttattctccc cataccagcc aaaaggctaa cagtgtcatg
     2041 attaagcaac ataagggggc aagaatatcc attgatcgct gcataattac ttttttctgt
     2101 tctttatttc gctggtttaa tagcgcatgg tggaattttt tgacttgtga atcaattgta
     2161 gtttgtattc tttctttggt ataaggtgca agaaaagcac gttgtttctt tatttcattt
     2221 tctatgtctt gtaacgtttt agaacgataa ttcttgttgg tagtctgctc ctctttcaac
     2281 gtgttggtca tggtttcttc gagtcggtct aatttccggt tgttcagtcg taagagttcg
     2341 ttgttctctt cgtacttctt ttgcagtttc gcgaatgcta gctcgagctc ttcgtagctc
     2401 gttggttcgt tgttgattgt aggctcgttc tcgtctgtcc agctcatgta aaatcgtctc
     2461 cttttcgtaa tcctttccta gtttgttgga tcgtactttt ttctttttgt ggatcaattc
     2521 gtaagtaaaa tttttcccac gattcaagca atgaatgccg aatgttttaa gagaatcaca
     2581 aaattggtta tacgttcgag tttttttgtt tttcattgtt tgatcaatcg caaaccgaat
     2641 ttcgtctttc caaacaggtt catttttttc ttttaatttg atttcagaca tggttcgttt
     2701 ttcgggtttc ttccctcgtt cagtgattgt taagtcatgt tcaagacaaa tctcgtcatt
     2761 taggtcataa catttgttta aaaatgcttg accatgggct tgaaatttgt tcccagtttc
     2821 cagattgacg gagttaatga caatgtggtt atgaatatgg tctttgtctg tatgtgtgta
     2881 aatcgcgatt tggtggtcag gggctatttt tttcgcatac tctgccccta gttgattggc
     2941 tttttcagcg gtaatttctc ctggtcgaaa agactggatc aataagtgtg cttgtacacg
     3001 atcgtctttt ccatacagca tgcgaatttg tttcatttcg cttttggcat aatcaatgtc
     3061 acagtttatc cctgttttga cggttgctcg tggttcggcg taattgatcg ctcggctaca
     3121 agaagtggtt cggcttactt taactgttgc catatctttt gtagctcctt ttgcatgatt
     3181 tttaggtttt cggataagcg ttgggcgtcg ccaggatcaa cgtcgtgttg gtgtgtgttg
     3241 caccacttgg caatttgatt gatattcccg ccagctttgg ctaaatcgcg cgcgattgcc
     3301 tgagcaatgt ctggcgccac tttcggtgtc accagacgcg cgttttgtgc cttggctttc
     3361 acgaaagccg gcacactcat ttgcaaagct tcagctgact gcttcagctt tgcaaattcg
     3421 gattcgctca ctcgaaaact gatttgtttt ggctcggatc gataggtata ttttttagaa
     3481 tgattgctag ccaaatattg attttgttcg ctcataagtc ccaccctttc agtacgtcct
     3541 acggacgtat cgttcgcaat accattgtac ctaaaaaggt acaataagta aagctcacta
     3601 aagctcacta aaggg
//
LOCUS       DAYYGY010000152         3590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000152 DAYYGY010000000
VERSION     DAYYGY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.157"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHA52_002873"
     CDS             complement(<1..2232)
                     /locus_tag="ACHA52_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8941512.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHA52_002874"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHA52_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1030 a          785 c          554 g         1221 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataacaat aacctttgtg
//
LOCUS       DAYYGY010000153         3162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000153 DAYYGY010000000
VERSION     DAYYGY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.158"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHA52_002875"
     CDS             complement(63..2555)
                     /locus_tag="ACHA52_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8941513.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHA52_002876"
     CDS             complement(2562..3029)
                     /locus_tag="ACHA52_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8941514.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAYYGY010000154         3071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000154 DAYYGY010000000
VERSION     DAYYGY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.159"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..696)
                     /locus_tag="ACHA52_002877"
     CDS             complement(334..696)
                     /locus_tag="ACHA52_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941515.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ACHA52_002878"
     CDS             complement(770..1150)
                     /locus_tag="ACHA52_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8941516.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="ACHA52_002879"
     CDS             1232..2407
                     /locus_tag="ACHA52_002879"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8941517.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="ACHA52_002880"
     CDS             complement(2484..3068)
                     /locus_tag="ACHA52_002880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8941518.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          958 a          587 c          504 g         1022 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat t
//
LOCUS       DAYYGY010000155         2758 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000155 DAYYGY010000000
VERSION     DAYYGY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.160"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..307
                     /locus_tag="ACHA52_002881"
     CDS             <1..307
                     /locus_tag="ACHA52_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8941519.1"
                     /translation="FDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSS
                     SYQQIPRKKTVIEFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            352..1326
                     /locus_tag="ACHA52_002882"
     CDS             352..1326
                     /locus_tag="ACHA52_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8941520.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            1349..>2758
                     /locus_tag="ACHA52_002883"
     CDS             1349..>2758
                     /locus_tag="ACHA52_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8941521.1"
                     /translation="MKKSRLEYSLINSSISMFIYILRIVIQFVGRSFFIYFLGAKYLG
                     LNGLFTNILSFLSLAELGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIG
                     IVGVLIIPLLPLFIKNSTDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAI
                     NDFAFLFFMNIVQVIALYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVV
                     SLDKQTKDEVKKNVIGNMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQ
                     NLCKQITNSITASVGNFAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISL
                     WVGAKYMLPMSTTILIVLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSL
                     LLLIVFDMGINGVLIGTIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELII
                     SAIVVFLLNEYLANGGSTFL"
BASE COUNT         1001 a          297 c          463 g          997 t
ORIGIN      
        1 tttcgatgtt atagaagcat ggcaatataa caaagagaag attttagatg cttttccaga
       61 tttgcatgat tcctattatc gccgactatg ttgggcatat ttttacgtgt tggataaaat
      121 ggttttatca agtagttatc aacaaattcc acgtaaaaaa acagtaattg aatttttaaa
      181 gaagaacaga aattttatat taacttataa gggattcact aaatcgcgca aaatagctat
      241 tattgctctg agctttagag tagattggta taggaaattg gttgaacaaa aaaataaaaa
      301 aaattagaat gtttaaatta gtaaataaaa gaaaaggaat tgaaaactaa aatgtgcaga
      361 ataagtataa tagttccagt ttataatgtt gaaaaatatt taaaaaagtg tgtcgattca
      421 attctaggcc aaacatttac cgattttgaa ttattattaa ttgatgatgg ttctacagat
      481 aattcaggaa gtatatgcga tgaacttgcg aaaacagaca accgaataaa agtgatacat
      541 aaggaaaatg ggggtctaag tgatgccaga aatattggta ttgaggtagc aaagggagat
      601 tttattgggt ttatagatag tgatgattac attgatgaag atatgtatgc atttttatat
      661 aacaatatat tgaaatatga tgcagagtta tcaatgtgtg gtatttatga cgtccataag
      721 aataaagaaa ttaaaaagtt aactcctttt tctcaattgg ttactaaatc tgaagcaatt
      781 gaattagtat tagatggtaa gctagttgtt gctaatgcag taagcaagct atataaaaaa
      841 gaattatttg agaatgtgag atatccaaaa ggaaaaattg ctgaagacgc agcagtaata
      901 ctgaaaatta tcaatcagtg taataaaatc catgttgata cttctcaaaa atattattat
      961 tatcatcgtg gagatagtat tactagtaaa aaatttacaa aaaaagattt tgatataatt
     1021 gatgtatgga atgagaatga aaaatggatt attaaaaatt atcctcaaat gtacgataaa
     1081 gtacatacta gagtttgttg ggcttacttt gttgtgttag ataaattagt tttaactgaa
     1141 gaaaaaggac tgtatcaaga aaaaaaacaa gtaagagagt atttaattga taatttcttt
     1201 tttattataa aaaataaacg attaactttt caaagaaaaa tatctatgtt attgcttact
     1261 tttggaattg ctgcgtataa aataccggtt aaatatcagt ataagaaagt aaaggctgtt
     1321 aattaatgct ttagttagga tgaattagat gaaaaaaagc aggttagagt attctttaat
     1381 aaattcttct atatcaatgt ttatatatat tttaaggata gtaatacaat ttgtaggtag
     1441 aagttttttt atttatttct taggagcgaa atatttagga ttaaatggtt tatttacaaa
     1501 tatacttagt ttcttgtcgt tggctgaact aggcataggc tcttctatta tttattccct
     1561 atacaaacca ttagcccaaa aggatgaaag taaagtaaag gctttaatga atttgtataa
     1621 gaagacatat gaatttattg gtatttttat cggcatagta ggagtattga ttattccgct
     1681 gttacctcta tttattaaaa atagcacaga cataccacat atgtatcgat attatgtatt
     1741 atttttatta aattcagttc ttagttattt tttcacgtat aaaagatcac ttgcaatagc
     1801 agatcaaaaa aattatctgg ttgcgattaa tgattttgcc tttttattct ttatgaacat
     1861 tgtacaagta attgctttgt acttttattc taattttact ctatttctaa tagtgcagat
     1921 tttgtttact ctattttcta atataaatat ttccagaaca gttgacaaga gatattcata
     1981 tttaaaagag aaagatgtag tttctctaga taagcaaaca aaagatgaag taaagaaaaa
     2041 tgtcataggt aatatgtcct caaaaatagg cggggttatt gtaatgggga cagataatat
     2101 attaatttct acatttgtta atttaacagc tgtaggaatt tattctaact attcattgat
     2161 tgtcacaagt gtgcaaaatt tatgtaagca aattactaat tctatcacgg caagtgtagg
     2221 aaattttgct gtatcaaata aaaaaaagga ttcatttgag ctatttaaaa aacatttttt
     2281 tatcaaccac tcactaatct ttttttcttc tatattgtta ttgactttaa tgaatccctt
     2341 cattagcttg tgggtaggag ccaaatacat gctacctatg tcaacgacta ttttaattgt
     2401 tttaaattat agtgtacagg tttatagaaa tacatccttc gtttttatag agtcatttgg
     2461 tttatattgg tctcaacgga aaaagcctat aattgaagca ggtgtaaact tgttattatc
     2521 tttgcttttg ttaatagttt ttgatatggg aatcaatgga gtacttatag ggactatcgg
     2581 atcgtctcta ggatttgtga tttggtatga agcatttatc atatataaaa cggtatttga
     2641 gaaacgtttt attcatttct ttttaacttt ctggcgaagt tttatagaat taataatttc
     2701 ggcaatagtc gtttttctat taaatgaata tttagcaaat ggaggcagta cgttcctt
//
LOCUS       DAYYGY010000156         2671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000156 DAYYGY010000000
VERSION     DAYYGY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.161"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..827)
                     /locus_tag="ACHA52_002884"
     CDS             complement(<1..827)
                     /locus_tag="ACHA52_002884"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM8941522.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSINMLAST"
     gene            complement(799..1695)
                     /locus_tag="ACHA52_002885"
     CDS             complement(799..1695)
                     /locus_tag="ACHA52_002885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8941523.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1685..>2671)
                     /locus_tag="ACHA52_002886"
     CDS             complement(1685..>2671)
                     /locus_tag="ACHA52_002886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941524.1"
                     /translation="NRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTIN
                     NIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNA
                     EKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNVGIN
                     YNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          924 a          443 c          323 g          981 t
ORIGIN      
        1 ctagtagaag ctaacatatt gatagaaaat atatatgagc ttaagctgat aacttttttt
       61 tcgtaaatat tcatttttga taatttatga actaaaaaaa gatttgctga aaaaaagaga
      121 gcaaacatca ctacgtgaga aatcgatgta ctgtttctat caaaatatgc atcatacttc
      181 gtatttataa atattgaact gatataactt tgtccaatag agaataggag caagctaagg
      241 attgtaacat atccagtaat ctttttatta atttttacaa taaaaaaagg gagataaaaa
      301 tatgcaagat tatggaacag tccagccact aagataaaaa aaatatactt aaatatagct
      361 ttgctgcctc gcaatttagt tagatgataa aaagctagta atatgaatat caatgcgcta
      421 aacatacgta cttgtattgc ttgtgaagta aaaccaaaaa taaaaaacaa gaacaaaact
      481 aaaaaaacat tagttgtgaa taatctgatg acatacaata aagctgagag taagacagta
      541 gaaataatca ataaaaaaat ctgatactga ctggaaataa aggtacatac gtacataaca
      601 gctctgaatc ccggttcaat actatctgaa ctaaagtcca taccaaattg tgctatccca
      661 ttatacaaga cagaatatgc ataataatca gcgttttgtg tgttaaatgc catgatgatc
      721 cacattatca aaagtagcac cccagacaaa atcttgttcg atgtactcca ttttttttgt
      781 gaaacactct tttcttgatt aatctccaat gacgcctctg tatccatctc gaacccccct
      841 tatagccgct ctaaatctac tgagcttatc agtctcaaaa gctattgcaa taaaaaattt
      901 caataatctt ttattcataa ggcgggaaaa ttccttatca cttttataca ttcttttcac
      961 caatgttgaa tttcttgcaa taaaatactt tcgtgttgaa ttatgattgt ctattttaat
     1021 tttttttgat cctattttta aattttctcc atctccaacg tgatgataaa aatagacttg
     1081 atttgttaca taaatgttat agccattatt tgcaaccctt ttacaaaatt caacatcaat
     1141 ctgatcaata aaaaattcat ccttcatatt tccaacatcg tcaattaatt cacttttgaa
     1201 aaacattcca gaggaatttg caaatgctat tttcatggaa ccactagttg aagcaaagtg
     1261 tttttttacg tatctccctc tctcattttt gcaggtaaaa gagacagatt catcattgct
     1321 attctcaaag taattgggaa caataatccc aatgtcctta tctttcgctt caatattggt
     1381 aaactcttct aataatttta aagaaagatt cttttgtatg atggtatctt gatctagtaa
     1441 aaggatataa tcacatttct cttttttggc aatttcaata cctatgtttt gagctgtcgc
     1501 aagcccaatg ttctcattca ttttttttaa aattaagttt tccatcattt caaattgaaa
     1561 agagttaata ttatctgagc cgttatccac gataataagt ttgttctgtt tctcttgtat
     1621 taacgtatcc actaataact ttatgttatt atctggattg taggtaacaa taattatccc
     1681 aattttattt ttcatcgttc atcaccttgt acacttcatt ccatttcatt ttcaatactt
     1741 gctcagagaa tatagtggcg taaatttttc taacattttc atttatgttt ttattacact
     1801 gataaaattt caacatctca ttttcatcaa cggtactatt ataattgatt ccgacattat
     1861 atcttttaac tatttctttc gtatctcctg ataaatttga aattataggt aatccaaacc
     1921 taaaatactc aatcgatttc agacttaaac caattatggt atttgactta aataaattta
     1981 gtccaaaatg acatttatcg agtataactt ttttttctgc attgtcatat ataacaccgc
     2041 agtcaacaaa ttcaatatga tttttttcac aaatatcagc taatttagtt ttgctttcac
     2101 cttttccaat aaagtaaaga tttattttgt ttccaaattt tcttttgcat gttttcaata
     2161 attcattaat cgcttctaaa tctacgatat tattaatcgt acctaaataa gcaaggttca
     2221 acgtagactc taagctttta ttgactattt tctctccata atcattttgg tcatctacat
     2281 tgaaagaaca tggataaatg gtcatatttt ttgattctcc ggaaattttt atgaattttt
     2341 tgaaataatc gcattctgtt ataactagac tagaattact gagatattga tctcgtagct
     2401 ttttccataa atcagtaaaa aatttatatt tgttccctat tcccggcatt gactctggcc
     2461 ataaatcata cacatcaaaa ataactcgat tgcatctact aaacttagca ataaaaaaaa
     2521 ctaaggtatt aggtggtatt ctacaataaa taatttcagg cttttctttt cgaataaata
     2581 ataatgtttt tattgaaaac catatatgag ataaaattcg cattacggat atattttttt
     2641 tatatccgat cgtttttatc acatgacgat t
//
LOCUS       DAYYGY010000157         2522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000157 DAYYGY010000000
VERSION     DAYYGY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.162"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..234
                     /locus_tag="ACHA52_002887"
     CDS             <1..234
                     /locus_tag="ACHA52_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8941525.1"
                     /translation="GVRSSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQD
                     RFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            194..439
                     /locus_tag="ACHA52_002888"
     CDS             194..439
                     /locus_tag="ACHA52_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941526.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            436..855
                     /locus_tag="ACHA52_002889"
     CDS             436..855
                     /locus_tag="ACHA52_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8941527.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            852..1409
                     /locus_tag="ACHA52_002890"
     CDS             852..1409
                     /locus_tag="ACHA52_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8941528.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1406..1702
                     /locus_tag="ACHA52_002891"
     CDS             1406..1702
                     /locus_tag="ACHA52_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941529.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1779..2192
                     /locus_tag="ACHA52_002892"
     CDS             1779..2192
                     /locus_tag="ACHA52_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8941530.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
BASE COUNT          892 a          382 c          580 g          668 t
ORIGIN      
        1 ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta ttacaacgaa
       61 attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaattg gagcaagaaa
      121 cttcatcaag atcgtttcaa atttgtagag aagtataaag aaatattaga agagtatttg
      181 aggaggacag caaatgataa aaaaactcgt tcaattcagc atggatttat atgatatcga
      241 atcaggagca acactatctg tggaatcgga ccatctaatc ataaattttg gtggaaagcg
      301 ccagattatt ttgtgggtag ttgatgatgt actgtttcca gaaattgttc atgatttcga
      361 agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta atggaattga ttgaaaaata
      421 cgaggaggac agcgaatgat accgaagttt agagcgtggg ataaacgaaa gaacgtaatg
      481 agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat tgaatattgg
      541 ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat gcaatccaca
      601 ggaatgaaag ataagaatgg tgtggatata tttgaagggg atatagtatt agtcagcgtg
      661 cgaaatggct tcgattactt agataataaa gtctgtattg tcaaaaattc aatagattat
      721 tccggattag tttgtgccac tgttgatgaa gacttagagt atcaaatttt taacacagag
      781 ctgtttgaag aatacacgta cgaagtcacc ggaaatatat acgagaatag cgagttattg
      841 gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt aaagcgatag
      901 gaaatgatgc aatagctgct tgctataacg agagtataaa ttcaggtatt acgttaatga
      961 aaaaaataga cgaaccgcag aaaccagtta ttccacaact cgtggccggt tggcttgaga
     1021 aatctacgga cccttttaca aaagctgaaa aaatagcgta tttaatcaaa tctaaagatg
     1081 gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg caagagcaag
     1141 gagaagaatt actcgcttgg gcaacaagac aatcatatga aacactattg agcctataca
     1201 acggctacga ggttgagaaa gagccgttat gggcaataaa gaatgccgat ggaaactatc
     1261 ttactaaatg tgctttatgg ggaaaagatg gagtaaatta tagttttgaa tgcaatccat
     1321 ctcatcgatt gcttttcact gataaagcaa cagcggatgc tgcagcattg ttggtgaatg
     1381 gaacagtgga agaggtggta gaaagatgaa actaaaagac ggattttacg ctagcagtca
     1441 cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac gcaagaaatc
     1501 aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt atccgttcag
     1561 aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa atacaatgaa
     1621 gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat tagttgacat
     1681 ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca ataaatccaa
     1741 cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta cgagaggtag
     1801 atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg cgtttggagc
     1861 ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat atgccgaagg
     1921 caccgaagca tggcaataag gcagaagacg cgattattca gatgatggat atagaaacgg
     1981 agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt cagatactct
     2041 actacagctt ctgtgtgcca gatagcttct caaactacag aattagccgt gaagtgggtt
     2101 attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt gcagaagcat
     2161 ataaacacgg aaaaataatt gcttataaat aatttggcgg ttttttggcg gaatgatggc
     2221 ggtttttaga catttaccag tgttattatg gtagtgtcga aagattagga aacaggatcg
     2281 acaaaataaa cgaagggagg caatctccct catcgtttta aacttcttct tagatagaca
     2341 gcaactaatt atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt catcggttgc
     2401 tgtttatttt gattagacag cagcactcac aaaactaaac atgttcttgt cgtctttcaa
     2461 ctgctgctgt ctattaattt atgtattgga gggaagaaaa atgaaattat cagtagaagg
     2521 ca
//
LOCUS       DAYYGY010000158         2406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000158 DAYYGY010000000
VERSION     DAYYGY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.163"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..707)
                     /locus_tag="ACHA52_002893"
                     /pseudo
     CDS             complement(100..707)
                     /locus_tag="ACHA52_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1230..1904
                     /locus_tag="ACHA52_002894"
                     /pseudo
     CDS             1230..1904
                     /locus_tag="ACHA52_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1897..2058
                     /locus_tag="ACHA52_002895"
     CDS             1897..2058
                     /locus_tag="ACHA52_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941531.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          861 a          366 c          401 g          778 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aatgaattaa gactattaaa tagataatta ttgtaactta aaaaacacag ctaaaaatta
     2161 gttgtgtttt ttattaggtt tacttttctg gtataaaagg aactagctac tttttcaaaa
     2221 tttctaaatc attcaatatg tagcgcttac taaattattc gccataaaat tttataattg
     2281 gatcactcat tgttttacct aagtcatatt taagtaataa ccggtgtttt tacataagaa
     2341 gacttcgggt attttttagt ttgattagct ctgatatacc tataaagaat atgctaaggt
     2401 aagaat
//
LOCUS       DAYYGY010000159         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000159 DAYYGY010000000
VERSION     DAYYGY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.164"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHA52_002896"
     CDS             316..570
                     /locus_tag="ACHA52_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8941532.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHA52_002897"
     CDS             571..888
                     /locus_tag="ACHA52_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8941533.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHA52_002898"
     CDS             1447..2382
                     /locus_tag="ACHA52_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941534.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAYYGY010000160         2387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000160 DAYYGY010000000
VERSION     DAYYGY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.165"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            206..2068
                     /locus_tag="ACHA52_002899"
     CDS             206..2068
                     /locus_tag="ACHA52_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8941535.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2387
                     /locus_tag="ACHA52_002900"
     CDS             2068..>2387
                     /locus_tag="ACHA52_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8941536.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETA"
BASE COUNT          856 a          353 c          466 g          712 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagc
//
LOCUS       DAYYGY010000161         2372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000161 DAYYGY010000000
VERSION     DAYYGY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.166"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(259..483)
                     /locus_tag="ACHA52_002901"
     CDS             complement(259..483)
                     /locus_tag="ACHA52_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941537.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            849..1133
                     /locus_tag="ACHA52_002902"
     CDS             849..1133
                     /locus_tag="ACHA52_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941538.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1136..1552
                     /locus_tag="ACHA52_002903"
     CDS             1136..1552
                     /locus_tag="ACHA52_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941539.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1689..>2372)
                     /locus_tag="ACHA52_002904"
     CDS             complement(1689..>2372)
                     /locus_tag="ACHA52_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8941540.1"
                     /translation="NELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKEL
                     SDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVN
                     PDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMD
                     IPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPK
                     ISLRNWLEE"
BASE COUNT          809 a          422 c          351 g          790 t
ORIGIN      
        1 atccgacctc tctggggctt tcgtgtgttt cgtgttcact tatttgcaat caaattataa
       61 cagaaataaa tatttatttc aaaaatatcc tacaaaagtc taaaatccgt aattatttct
      121 tatatgcaac atttacaaat aaagtcgctc tgagagctcg tatttttcga tttaagacgt
      181 tttatttagt ttttggtgta tttcattggt ccaagctaaa aaactcttag aagcgatttt
      241 agagccattt aaacccactt aattatattc tccatgaaat tcgctccatg cgtcaaaagc
      301 tgcgtctgaa attccgccgt cttcgttcac tagttgcatt aatttttcca acgatcgttt
      361 tctttcgggt gtcatatttt tatattccaa caatttttcg tctgttaaat cgaacttgat
      421 tgcttgaatt gcttgctcta atttttctaa gttttgaaaa tattcgtaag gaaatttttc
      481 cattttttct ttctccagtc tcacaatttt tctagaaata aaatcacttc tttgccgtct
      541 atactctcaa aaaaaatgtc gtttgaaaaa ctaaataaat ttttgatcgt gcatttttaa
      601 atttggttat cgattacacg tgcagcactc ccccccgaat tttaaagagc ataaggaaac
      661 gtgtacccac tttctaaaaa aagtgggtag ataggctgtg cctatcacgt ttctcagaaa
      721 aaaatttgat atcacttttt cgccatcgtg ggtagcttat gtatagcatt ttggcaaaaa
      781 acagctatac aaatatagta tttgtatggt atacataaat tatacataat tgcaaaagga
      841 gattttttat ggacatttta gttcggaata ttgatccaaa gtttgttgca acaatagaca
      901 aacgttgcca agaactcacg aataaaacag gagaaaaatg gagtcgaaat aaatatctaa
      961 aagttttaat cgaaagtgat tttgaccgtc aacttacgac ttacaaaaaa gaccaattcg
     1021 atttattgct cgataaattt attgctatcc aaacagaaaa tactcaaata ttaagcgagt
     1081 atgtctacac aaataatcaa atcattgaaa tgcttatcgg aggaatgata taaccatgag
     1141 aaaaacttct gtccaaaaac atgttacttt gtaccctagt catgttgacg ttttagattc
     1201 catcatcgaa aaaaatccag atatcaataa ctactcggaa gccattcgct ttttatgtat
     1261 gagttatgaa cccgatacca acacgaccac taccagcaat caggcaaaac taaatgctat
     1321 gggaaaagaa atttccatca tgactgaaat catcgcagaa tttgctgatt tatatctaaa
     1381 agatacaggc gttcttattg gcacaaattc taaagtatat caagaagtga aagaaaaaat
     1441 cgaagctaaa ctaaatccat caaatcgaaa tcgaccaaag aatcatcaaa aagaaaccag
     1501 tcaaacagtc acttctagaa atttcgaccc aacaaatttt ctaaaacgat aaaaaacaga
     1561 taggagttat tcctatctgt tttttattta gaagccccat acaatatgct agaatgtact
     1621 agcaatctta gtcggttgta agggacttgt ctacttgctt aatgtaaggg gggcgagttc
     1681 ttttttattt attcttctaa ccagttacgc agagaaatct ttggcaaatc tgacgttagg
     1741 cttgaaaaag taaattctaa tttggcgatt ttattgccct tttttgcttt tatttttgtg
     1801 actttaagat cattgaagta tttttttagt tctcgtaatg caggatttaa tactttttga
     1861 tcaatgttac ccatttgata gtattctggg atatccatta agctcctaaa ctcttcaata
     1921 cgaactacat aataaccagt agaacggtac tgcataagca gtcggaatag agtttttgca
     1981 tacgtagaac gcaacgacgt gaaagcagat aattcaaatc tactaaattc agctgttaaa
     2041 ccattaataa tatattctag gtccggatta acactgactt caacaaactt ttttgtttta
     2101 ttgatttcaa aaccagtaaa caaaacaaag tagcgaatag aatcctcatc ttcagtacgg
     2161 taagtaagat taagcatatc tttatagagc ttttctaaat caccgacaaa ggcttttgta
     2221 gcagtcatct tataatcact taactctttt aattcctcga aagaaaatct aacttttttc
     2281 aaaccacgat ctttcatacg agcgcataat gcaaaaaata aatccatctg cttagcattg
     2341 aaatttttca gcggtactaa attcaattca tt
//
LOCUS       DAYYGY010000162         2350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000162 DAYYGY010000000
VERSION     DAYYGY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.167"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..543
                     /locus_tag="ACHA52_002905"
     CDS             <1..543
                     /locus_tag="ACHA52_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8941541.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1016..1588)
                     /locus_tag="ACHA52_002906"
     CDS             complement(1016..1588)
                     /locus_tag="ACHA52_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8941542.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1604..2209)
                     /gene="fic"
                     /locus_tag="ACHA52_002907"
     CDS             complement(1604..2209)
                     /gene="fic"
                     /locus_tag="ACHA52_002907"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8941543.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          467 c          359 g          783 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
      601 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
      661 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
      721 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
      781 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
      841 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
      901 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
      961 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1021 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1081 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1141 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1201 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1261 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     1321 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     1381 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     1441 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     1501 tctctacgca gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     1561 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     1621 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     1681 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     1741 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     1801 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     1861 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     1921 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     1981 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2041 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2101 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2161 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2221 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     2281 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
     2341 cacagtctag
//
LOCUS       DAYYGY010000163         2346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000163 DAYYGY010000000
VERSION     DAYYGY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.168"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            531..1562
                     /locus_tag="ACHA52_002908"
     CDS             531..1562
                     /locus_tag="ACHA52_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8941544.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            1671..2096
                     /locus_tag="ACHA52_002909"
                     /pseudo
     CDS             1671..2096
                     /locus_tag="ACHA52_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            2209..>2346
                     /locus_tag="ACHA52_002910"
     CDS             2209..>2346
                     /locus_tag="ACHA52_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8941545.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          850 a          403 c          374 g          719 t
ORIGIN      
        1 tgaaaaattt catataattt ccactcgttg tgatggttta atttatccaa tccatcaata
       61 agcaaacaac ttatataaaa aaggattaat tctcaatata attgctccta ccatagaata
      121 aatcttcatg taaagtaatg aagtaccaac tatttctaat acttctttaa gaattatccc
      181 aacaatatcg ttagacaaac cattattgaa taaatggaga gccaatcgat tctgaaaaaa
      241 tgttttctaa tttttcggaa ataaaagaaa gagcctcctg ccattagaac gaaccaaatc
      301 catttcattg ctcatgaagt aaccacatag ctaaaaagag gataaatcaa ttaaaagatc
      361 aaaagaatga ctgcactaat aaaataacta aaaatgtatc ttcttacttc atatctttta
      421 gctagtactt agttaaaaat ttgcttccta agatcaagac aaactgtcta tcttcatttg
      481 atgaaatgcg gtaaaatata gaaaatgaac actattggag ggaatttgta atggacagca
      541 aaactagaaa acaactgcaa gaaaagttaa agcaacgtac acacttgctc ccttccgtct
      601 ttcaacagtt tttagaagaa aatcgttctt ccctttcatt aggcagtcgc tatgaatacg
      661 caaaagattt tcatctgttt actgattatt tgagaaaagt attttctgaa gaagtggatg
      721 atcagctgat tatccattta gaaaaacaag attatcagaa tttcttagct aaaatctatc
      781 gacatactcg ttcgttccaa actacagcga accaagaaac ggagcaattg tttgaaaata
      841 gctattacgg gatttcaagg aagcaatatt cattaaatca ttttttacga tttttgtatc
      901 aaaaaggact gattgaagaa gagatttctc ttttgggtac aagtttaccg gaaacaacca
      961 aagcagctcc gcgatatatc gatgcagaac ttttcaaaga ttttgagcat ttgtttatcc
     1021 cgattgaagg ctttcaaaca agcagagaag ctagctttga agaaaagaat agaccgagga
     1081 accttgcaat tactgccatt cttctgtatt gcggattaaa gatccatgaa gtagtagaac
     1141 ttaaacgtaa agatgtatct ttgaccaagc agattcttaa tctgaataga aaagaaaaca
     1201 gactgaataa agtaccaata ccaaaagaag cgatgccatt tctggagaac tattttaagt
     1261 taacagcaca aaatacggac gaaaattctt ttgtttttcg ttcctcacat gatcagcaaa
     1321 tttctcctcg gactattcgc caaattttga gcaaaattac agtatataaa aacgtatcgc
     1381 cagaactatg cagaaaaaca tatcgttatt atgcagaact atatttagat gatgctgacg
     1441 cggtaaatta tttatgtggt aatcgatacc tctcatccca ttcttttcaa gatatctggg
     1501 aaaagatggt tgatttttct tatcacgaat tagcggcatt cgttcgggtt tcttccatat
     1561 aaaacaaatg tatgatcttt ttttcacaat aaactatgtt attatataaa ggattatgtt
     1621 acgattatct tgaatttgta tattacttaa caaaagataa tggagtgtga atgatggaga
     1681 ccataacgta ttcccctttg aacttattta cgaattttca agaagctgca gctaactttt
     1741 ccgagacccc gattatcttt gatgagccac tttcagcttt cccagaatta ggacttgaaa
     1801 caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc ttatcaattg gctcatcttg
     1861 gagtgaaatc cggagacaaa gttattattt ataagagttc cgcttttgac acataccttc
     1921 tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat gacttcttat catctaccaa
     1981 caacaacaat ggaagtattt atcgatcgtt tagaagaccc tttcatttta tttgatgatg
     2041 agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa atcaaaacag attcctaacc
     2101 cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa
     2161 aaaaacgcat atagtagtct caaaactccc aagaaatgag gactacatat gcgttcatca
     2221 aaatatactg aacattatac agatactttt ataacattca aggaaattat gagaactgtt
     2281 tctaacatat accacaattg tgtaccagat aagattaaaa accgaagaaa tactgatcaa
     2341 ctgaag
//
LOCUS       DAYYGY010000164         2339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000164 DAYYGY010000000
VERSION     DAYYGY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.169"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..345
                     /locus_tag="ACHA52_002911"
     CDS             133..345
                     /locus_tag="ACHA52_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941546.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            507..1025
                     /locus_tag="ACHA52_002912"
     CDS             507..1025
                     /locus_tag="ACHA52_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8941547.1"
                     /translation="MNKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1032..1544
                     /locus_tag="ACHA52_002913"
     CDS             1032..1544
                     /locus_tag="ACHA52_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941548.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1815..>2339
                     /locus_tag="ACHA52_002914"
     CDS             1815..>2339
                     /locus_tag="ACHA52_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8941549.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          893 a          298 c          383 g          765 t
ORIGIN      
        1 aaaaacacta tatattccga aaagtgagta tctccattca taaaataatc aacatattta
       61 aaaaatctaa ttagcttttt tagtcaatct aaagttcttt attctatgat ataatgttac
      121 tgtagaaagt ttgtggcggt ttggcaaatc caattgaagg agcgtggtgc ctatgggctt
      181 attagcttat cggacgtact ctgtagaaag gaaatgcctg atttgttctc attggatcaa
      241 atcattgcta ttatcggagc agcagcagta gttattactg ccgtagcgaa agctagcgat
      301 tcgattttag cttggttaaa gttcaaaaaa gaacaaaaaa aatagagccg tctcaacttt
      361 tggcgaggta gacgagctct aaagctttat ttacatgcca tccgctttga acggactaca
      421 ctgggcatgt gacagcatgc cctttcattt atattatatc tcattctttt atataatgaa
      481 agtaaataaa tcatggagga taaaatatga ataaatttaa tagattaaca ttaataaatc
      541 agtttgaaat tttaaaagct ttaaatccta acgaagaaaa atactatgct gaaaaaatag
      601 aaatactaac agaaggttac gaatatcact atagtgaaat atttgaaaat atttctgatc
      661 cattgccaga agaagattcg cggtttgttc tagacatatt aaatatgtat agagatatta
      721 cttttagtaa aaataaatta aaagatataa attcaattga taattattat atacaattta
      781 aaggttttga ctttaatagc tctaccgaat ttaagttagc actttatgca caatttttta
      841 ttgaaaaatt aaataggttt caagaaatag ttgaagatag tgattttaac acttttaata
      901 gccataaacc gatgagaggt acttatataa aatttctcga aaattataat gatttaaaat
      961 ctaccaataa ttatcaattt ggaaacttgt cttatgagat gataagtaaa gttctaagtt
     1021 tgtgatgata aatgaaatta aatagtataa atgatttatt tgctattata ggtgttattg
     1081 ctaacatact tttttcgtgt ttgaatttta gatttactca aaagaacaaa aagaacaaac
     1141 tcagtataga acaaatgaaa aaagacattg aaactgattt aaaaaatgac cataaagaaa
     1201 gaagaaaaat tgaagaatat acttctttaa tggaaaagtg taaagcaact gatgcaattt
     1261 ttagcttttt aagaaaaagt gatgagataa tcacacgtga aaatatagat ttagaaaatg
     1321 agattagtaa acttgaatct atcttttccg atataaattc tcttaagtat ttaatcgtcg
     1381 aagaaaattc ggatagcttt ttagaatttg aaaaatatct aacagatctt atagataata
     1441 gaaataatga attaatgaga caaaacattg caagattcct tactaaatta gtttctgaat
     1501 ggaaaagtat tatttctcaa aatatggata aattaagata ttaaggatgt tagttttaaa
     1561 ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat acttaaagga
     1621 ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta tttgttgttt
     1681 cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga tttttcagcg
     1741 gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa tagattggat
     1801 aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat caaagtttag
     1861 ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct gaagacgatt
     1921 taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca gagctgcaag
     1981 agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt gatcgattag
     2041 caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa ggtgcttcta
     2101 ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta agagacttgc
     2161 taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg aaaaaaatca
     2221 aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat actggtaaaa
     2281 agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat gaaattgtt
//
LOCUS       DAYYGY010000165         2255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000165 DAYYGY010000000
VERSION     DAYYGY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.170"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..>2255)
                     /locus_tag="ACHA52_002915"
     CDS             complement(144..>2255)
                     /locus_tag="ACHA52_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8941550.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          765 t
ORIGIN      
        1 gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg tatgaagtca
       61 aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac tcgaaactaa
      121 gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata cgcgtccatt
      181 atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt cggcaagaca
      241 acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt actattgact
      301 gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt tgcggaaagg
      361 tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc atggacaact
      421 tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac cgtattttct
      481 actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc attgccatag
      541 atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt tgaagcatat
      601 tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc ggtaaagtta
      661 cctggagcat accattcttt gccattatcc gcattgaact tcttcatata atcaacagaa
      721 cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc aagaccaaac
      781 tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa actaggtagt
      841 cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat catatacgta
      901 ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct tcgattaaag
      961 acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa aacttgattt
     1021 ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac tacagaccct
     1081 ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt ggaaccttgt
     1141 tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat agccactgca
     1201 taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata attccttttc
     1261 aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa attagaacct
     1321 ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc aagggaaact
     1381 tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa ataactttgt
     1441 cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc atctgctgga
     1501 agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact tgctgcatta
     1561 tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc gcgttctgcc
     1621 acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt gtttaattca
     1681 tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc gtttaaatta
     1741 atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt ccctaattgc
     1801 ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc agccagccaa
     1861 aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat cagcgagttc
     1921 agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct cgtatatgtg
     1981 atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta gatcattccg
     2041 cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc ctccatctct
     2101 tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa taacgtaaat
     2161 ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa cgttttgttc
     2221 ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DAYYGY010000166         2172 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000166 DAYYGY010000000
VERSION     DAYYGY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.172"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..2172
                     /gene="acm"
                     /locus_tag="ACHA52_002916"
     CDS             7..2172
                     /gene="acm"
                     /locus_tag="ACHA52_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8941551.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          856 a          348 c          419 g          549 t
ORIGIN      
        1 gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat tactaatata
       61 acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag cagtaatgtc
      121 acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa agtaaaattc
      181 tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat caattggcca
      241 agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat gattgaaaat
      301 aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt taatgaaaat
      361 attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc cagaaacgta
      421 accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga taaaacagct
      481 actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta ttataaaaca
      541 ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat taacaataac
      601 ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg acaaagatta
      661 gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt ttatagagga
      721 gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa tttttcagta
      781 actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag gaaaataatg
      841 gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa tcattcggaa
      901 gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa tcatacagtt
      961 aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt gaagattttc
     1021 aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt aagacgagca
     1081 gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga acagggagaa
     1141 gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga agcacccaat
     1201 tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa cgaaaatgat
     1261 acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat cacagcaact
     1321 aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt attcagagca
     1381 agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga caatggaata
     1441 acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga atatacgttt
     1501 aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta tcaaaaaatt
     1561 gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc aggacaaaaa
     1621 acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac agtaaatcta
     1681 ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga ttggcgctat
     1741 cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac tgtaacggaa
     1801 gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa cagttataca
     1861 ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa aaattttcca
     1921 gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca aatatttgct
     1981 catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc tgtacctaaa
     2041 cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa atctagtttt
     2101 gctgtaatca ttggtttatt gatagtaact atctgtgctg ggatattctt acaaaaatat
     2161 aaaaaagttt ag
//
LOCUS       DAYYGY010000167         2168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000167 DAYYGY010000000
VERSION     DAYYGY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.173"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..575
                     /locus_tag="ACHA52_002917"
     CDS             12..575
                     /locus_tag="ACHA52_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941552.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(636..1001)
                     /locus_tag="ACHA52_002918"
     CDS             complement(636..1001)
                     /locus_tag="ACHA52_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8941553.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1077..1322)
                     /locus_tag="ACHA52_002919"
     CDS             complement(1077..1322)
                     /locus_tag="ACHA52_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8941554.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1392..1646)
                     /locus_tag="ACHA52_002920"
     CDS             complement(1392..1646)
                     /locus_tag="ACHA52_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941555.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1810..2133)
                     /locus_tag="ACHA52_002921"
     CDS             complement(1810..2133)
                     /locus_tag="ACHA52_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941556.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          813 a          363 c          377 g          615 t
ORIGIN      
        1 taaaaataac tgtgttatat attaaaaaag gcataatagt tataagcttt tttaaagaat
       61 atttagaaat tattaatttt cgtctgaaaa caaaaaaaga aagcgttatt attttagtct
      121 attttcaggg ggggaacctt atgataaaaa aactattttt actatcaata ttaacgctag
      181 caatggcagg ttgttctgac tctggcttag caactgaact gatcacaaaa tcaactgaag
      241 ccacgacaga ggagttaaaa tgtggaaacg atcacagtga ctacagaggc ttaaatcgac
      301 gatgcatggg aatattaggc agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt
      361 gtagaagaag caacagaaaa atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat
      421 cgtatacaga cgtcgaagat gcggtacttt taaaccacgt caggagatgt tcatttatgt
      481 ctagggaagt tggcgatgaa ccaaaactag agtacgcttt tagttttgca cgtgaggatg
      541 aagataaccc taaaacttac gacattttag cttgatctaa gatggttaaa gctttttaat
      601 gaagtaagag gagatagaag gcttttctag ttatattagg taagataaat tcctttttgc
      661 tccaaatttg ctaaacattc tgctagataa acttcatcgt gttccggata aatcggcacg
      721 ttgcgctcta cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg atacatagga
      781 tttttccact ctggtgctgg gctgtaacca cgtttttcca tttcatccat gacaagctga
      841 tgatattgga ataatctata aggagaatag tgaaaaacat aatccacagt agcatgtttc
      901 tttccccagc catttcctct taatgcacag cattcccgat gttgtcctaa aagctgttga
      961 cgaggaagct ttgaaatcaa tttttcatgc cataatcgca tagaaatgtc tcctttatat
     1021 atagattgtt aaccttagac gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac
     1081 ttcagtttgc ttgctgtttc tgagcgacca attaggtaat caacggaaat atcgtaaaag
     1141 tcagcgatat agattaattc aatgatgctt ggaatttctt ctccttcttc gtacttacga
     1201 tatttgaata aaggaatact taacgcgcta gaaacagcta cttgagataa agatcggcca
     1261 gttcttaaga aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc
     1321 atttaatcac ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca
     1381 atcaatagta ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa
     1441 gacaaaacca gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa
     1501 ttaaaaactt tccacataaa gtagatagcc ggaagagtca aaatagctaa ataaaaccgt
     1561 tctttgttcc aattcataag ataaacaata cttgcgacca gtgcgaccgc aggcacccaa
     1621 aacaaaactc tcaccaacgt aatcatgtaa cggactcctc aacgattcat ataataaaag
     1681 ataacatgtt attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc
     1741 aatgaaaaat gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat
     1801 gtgtcttatc tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa
     1861 aaagaggacg atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac
     1921 atatttaaca aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat
     1981 catgacaaga agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc
     2041 tgaggtttga taatttaatt ggagaaaata agctgctgcc gcaactaaat cgaataaaat
     2101 cgcaataaat ggcaaaataa tcactcctaa cattgagaat aaataaaaga ataacatttt
     2161 ttctaaaa
//
LOCUS       DAYYGY010000168         2118 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000168 DAYYGY010000000
VERSION     DAYYGY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.174"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHA52_002922"
     CDS             278..820
                     /locus_tag="ACHA52_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941557.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACHA52_002923"
     CDS             976..1425
                     /locus_tag="ACHA52_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8941558.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2118)
                     /locus_tag="ACHA52_002924"
     CDS             complement(1455..>2118)
                     /locus_tag="ACHA52_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM8941559.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          752 a          338 c          401 g          627 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgaccga ttcggcgc
//
LOCUS       DAYYGY010000169         2061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000169 DAYYGY010000000
VERSION     DAYYGY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.175"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..320
                     /locus_tag="ACHA52_002925"
                     /pseudo
     CDS             1..320
                     /locus_tag="ACHA52_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(592..882)
                     /locus_tag="ACHA52_002926"
     CDS             complement(592..882)
                     /locus_tag="ACHA52_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941560.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1090..1781)
                     /locus_tag="ACHA52_002927"
                     /pseudo
     CDS             complement(1090..1781)
                     /locus_tag="ACHA52_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          581 a          387 c          304 g          789 t
ORIGIN      
        1 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
       61 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
      121 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
      181 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
      241 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
      301 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      361 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      421 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      481 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      541 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      601 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      661 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      721 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      781 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
      841 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
      901 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
      961 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     1021 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
     1081 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
     1141 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
     1201 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgatttctac atttgttttt
     1261 tcttgtgtca gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa
     1321 agttttgctt gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca
     1381 tcattttttt ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt
     1441 tgtgcatcct tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt
     1501 ttttggtttt ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct
     1561 tgcaatgtct gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta
     1621 tctgtcaatg tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct
     1681 ccaccccaca aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga
     1741 aatgcaacga caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc
     1801 aatactttta catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta
     1861 catatgtaat tttaatataa atactatcaa atactcattt aatcctcctt ttttatctat
     1921 ttaaaaaatc ttttttatag agaaaaatgc aggttaatac tttcttaaat aaatatttat
     1981 ggtgaacaaa aacaaaaaag caagttttat taaacgtaac ccttttcatt tcatagatta
     2041 ctgtatttat tacaaaaaac g
//
LOCUS       DAYYGY010000170         2019 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000170 DAYYGY010000000
VERSION     DAYYGY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.176"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..149)
                     /locus_tag="ACHA52_002928"
     tRNA            complement(77..149)
                     /locus_tag="ACHA52_002928"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="ACHA52_002929"
     CDS             530..1099
                     /locus_tag="ACHA52_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8941561.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="ACHA52_002930"
     CDS             1086..1946
                     /locus_tag="ACHA52_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8941562.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
BASE COUNT          712 a          349 c          413 g          545 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataa
//
LOCUS       DAYYGY010000171         1968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000171 DAYYGY010000000
VERSION     DAYYGY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.177"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHA52_002931"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHA52_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8941563.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHA52_002932"
     CDS             852..1040
                     /locus_tag="ACHA52_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8941564.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHA52_002933"
     CDS             1053..1610
                     /locus_tag="ACHA52_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8941565.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHA52_002934"
     CDS             1642..1884
                     /locus_tag="ACHA52_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8941566.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          718 a          305 c          404 g          541 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatt
//
LOCUS       DAYYGY010000172         1935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000172 DAYYGY010000000
VERSION     DAYYGY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.178"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..245)
                     /locus_tag="ACHA52_002935"
     CDS             complement(<1..245)
                     /locus_tag="ACHA52_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8941567.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            483..1652
                     /locus_tag="ACHA52_002936"
     CDS             483..1652
                     /locus_tag="ACHA52_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8941568.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          688 a          323 c          300 g          624 t
ORIGIN      
        1 tcttgccata cctcctgaat ttgaccatca ggagtcgtaa tgaaaatagt atcattttca
       61 tctactaatt ctcttaagtt taaatattgt ttactagatg taaaattcga agctgctaat
      121 gacgagtctt cgcttttact ataaaatcct gttacttgta ttttatgttc ctggaaatac
      181 ttcccgaatg aacaacctac cttgccggct ccaataaaac caacgttcat aattatcacc
      241 tccataaaga agtgagacta caagtcccat tcttacgata tgagcttaaa aataaaaatt
      301 aaatcaaata tacagtatgt ttttttcaaa tatcatccat agttaattta tcataatatc
      361 ccatatttta aaataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
      421 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      481 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      541 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      601 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      661 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      721 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      781 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      841 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      901 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      961 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
     1021 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
     1081 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
     1141 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
     1201 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
     1261 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
     1321 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1381 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1441 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1501 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1561 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1621 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1681 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1741 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1801 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1861 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1921 ccatggataa taata
//
LOCUS       DAYYGY010000173         1928 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000173 DAYYGY010000000
VERSION     DAYYGY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.179"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            198..1163
                     /locus_tag="ACHA52_002937"
     CDS             198..1163
                     /locus_tag="ACHA52_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8941569.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMQLNEGNGFVKSVGKNGWQIVDQYEENIAYIELLQF
                     HEGKARIDFNPNKIGQFLASSMKNFIHDLFLEPHFSRADIACDMVNVPDEFIMQYRVV
                     DPVSFKPIYGRSGKLESAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1363..1545
                     /locus_tag="ACHA52_002938"
                     /pseudo
     CDS             1363..1545
                     /locus_tag="ACHA52_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          630 a          294 c          438 g          566 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta cttcacctag
       61 ttccgtaact caagttcatt atacgaatat ttttaggttt cttcaagcta gagatttttg
      121 gatgtggatt tttagagtta cgtgaactct ggaaattctt tccaagaatc tcttttttcg
      181 ttgaggagga gctatttttg agtaaaaaag gcataatcgt aaatgagtgg gaaaaactta
      241 accgtatttc taacaggcgg ttaaatgttc aaaatttgaa caccccccta aaattacagt
      301 ggtcaatcga ccgaattacc attgttggaa aattgaaaga aaatatttat tatcacacac
      361 caaatgatgt cttgatttta aattttgagc aattgatgca attgaatgag gggaatgggt
      421 ttgtcaaatc agttggtaaa aatggctggc aaattgtcga ccaatacgaa gaaaatatcg
      481 cgtatattga gctcttgcaa tttcatgaag ggaaagcacg aattgatttt aatcccaata
      541 aaattggtca gtttttagcc agttcgatga aaaatttcat tcatgatttg tttttagaac
      601 cccatttttc gcgggcagat atcgcttgcg atatggttaa tgttccggat gagtttatta
      661 tgcaatatcg tgtggttgac ccggtatcgt tcaaaccgat ttacgggcgt tctggaaaac
      721 tagaatcagc ttattggggt agccgtgcca gcgaacgaca aattcggcta tacaataaaa
      781 aattggaaca agaaaccaaa cgaaaagttg taccaaagga agttgagact tggtggcggt
      841 tagaaatgca actacggcgt ggcaaggcaa ccgattggca taatatggtt caagaaagtt
      901 tgaatagttt tgcgagcctg cattttttgc cgattgatac aacacctaca gatagaataa
      961 tgctcgctgg tttaatgtct gatcaaaatt tctggtctct tttgtcgagg aattcaaaat
     1021 ataaatatcg ggaattgctg aaagaagaaa gtcaaaatga tgaattgacg aatcatttgc
     1081 gagaaacgtt tgcagaatcg gcagacaatt tgaaacaaga attagacacg tggcttttgg
     1141 gtttggacgt gacagagaag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
     1201 tacgtcaatg tttaatgacg tgagtttacg ataagcgatt attttgcgtg aagcaaaata
     1261 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
     1321 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
     1381 tgttactctt tgtcaaagag taacagaaag gtttacactc tttttattag cgacagcgcg
     1441 aaggaggggt ttaacttggg gtttagtatt tcgttcaaaa agggaacaaa aaatacaagc
     1501 atttctcata ataatcgtga gttaactgat agccaaaaaa ttaatagttt gtctggtaga
     1561 aaataattag cagatttaca agtgggaaat aaaaagctga aagaagaaaa ttgcatttca
     1621 aactacattt tctacaataa ctctaaaatg gcatattttg cgttctgagc ggttttagcg
     1681 tttatttcgt ttagttatcg gcacaatcgg tgaaacacgt tagaggagcg aaaatggagc
     1741 ctgtaaggcg actggtagcg gaacggtcgt gttagattgt gtagccgata gctgaacgaa
     1801 ataaatgctg gcagcgccat tctatttcgg caggaggagg ctcaaggggt tcggggaatg
     1861 aaattccctg aggtcttttt attttttcta gaggagtgct ataatcaaag aaaatgagaa
     1921 aaaagagt
//
LOCUS       DAYYGY010000174         1838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000174 DAYYGY010000000
VERSION     DAYYGY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.180"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..390
                     /locus_tag="ACHA52_002939"
     CDS             193..390
                     /locus_tag="ACHA52_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941570.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            496..978
                     /locus_tag="ACHA52_002940"
     CDS             496..978
                     /locus_tag="ACHA52_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941571.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1036..1632)
                     /locus_tag="ACHA52_002941"
     CDS             complement(1036..1632)
                     /locus_tag="ACHA52_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8941572.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          569 a          276 c          368 g          625 t
ORIGIN      
        1 aggaatatgt tttaattttt tgaaaaaaat tcttctaaat acaacagctg ttttaattgt
       61 gaaaacgaaa acagtacgct ataatgcctg taaggttgat ttttaactgt aaataaaaat
      121 caatgagtta aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt
      181 aggaggattt tgatgacgga agaaatgatt aatttaggag aacaatatgc atgcaaacca
      241 attggtttta caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg
      301 gtcaaagttg cccaatgtgc tgcagaggac caagaattat tagatgaaaa agcaagcatg
      361 gttgttgcaa aatatgatac atttgaataa agaaaacaat tggacgtgcg gcgtgctgca
      421 agtccaattt ctttgagaag gtaaatcagt attcttttcc tattttctgg agaagaaaaa
      481 gcgaggcgag ttgtcatggt aacattcttt acggttctat tgtttttatt cgttgttgtg
      541 atgtttttat ttctttggct ggttcgaaaa gaaattattt atcggactgt gcgaaatcga
      601 tgggtctact tggtgatacc cttcttagtg tcactagtca tttggtatac actcatcagt
      661 catcctacag cagatgaatt ggcaaaaggg atcttgtcag caatgatttt tattagtttt
      721 ttgttggaca gtagaggaat cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa
      781 ggtgttcctt tttctgagat caataaaatt gtattatatc agccaaaagg ttctaaaatc
      841 gtaaaaatga attttttccg aaacggatgg cgaggaccga tgctgaaatt ttctgcatca
      901 ttagaagaat tagttccatt tttgtcccaa cgcttaaacg atgaggcaga gattgatatt
      961 atgattgatc cagaataaaa acagaggttg cgacaagatg tttgtcacaa cctctgtttt
     1021 ttatttcaaa taaaattatc caaatacgcg gaagcgatcg gcatcgttca tgtattcttt
     1081 ttccccagcc ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt tccagtttga
     1141 agggatattc cattcttttg caactgcttc gtcaataact gggttgtaat gttgcaagtt
     1201 tgcgcccaat cctttatcaa ctaatgctac ccaagtatta gctgttgcga ttccgtttga
     1261 ttgttctgac caatctggga agttgtctgc atataattca aattgttctt gtagaccttt
     1321 taccacgtca gtatctttga agaataatac tgtaccataa ccatttttga aaccagctaa
     1381 tttgttttgt gtatttggga aagcttctgc tggagtcaat gggcgaagtg cttcttcagt
     1441 gatttcccaa agtttttcat gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc
     1501 attgaaagca gttgggcttt ctttaattgc ttctttgatc aaagttgtta attcttcatt
     1561 agaaagtgaa acattacgtc ctagatgata gattgaacgg cggtttttca aagtgtcagt
     1621 aaaagttgtc ataataaaat ttctcctttg atttgcgttt tgcatttttt taattgatta
     1681 cgaaatgaag tatagcacct tacaaaaagt aagtaaacag atatgctcac aaaaaataag
     1741 cgaagttcaa aaaacaattt taataagaaa aatcgttatg taaggatttt cttagttatc
     1801 gctttcattg ttgtgtttta ggaatttttg cttatttg
//
LOCUS       DAYYGY010000175         1792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000175 DAYYGY010000000
VERSION     DAYYGY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.181"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..368
                     /locus_tag="ACHA52_002942"
     CDS             <1..368
                     /locus_tag="ACHA52_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8941573.1"
                     /translation="LDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFL
                     REQLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEA
                     TETMDEQTKNWKKWWQLWK"
     gene            350..553
                     /locus_tag="ACHA52_002943"
     CDS             350..553
                     /locus_tag="ACHA52_002943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941574.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            1008..1232
                     /locus_tag="ACHA52_002944"
     CDS             1008..1232
                     /locus_tag="ACHA52_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8941575.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            1252..1539
                     /locus_tag="ACHA52_002945"
     CDS             1252..1539
                     /locus_tag="ACHA52_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8941576.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT          737 a          245 c          312 g          498 t
ORIGIN      
        1 acttagacaa tcaaaggttg ctagataagt tgcataaaca tgaacaaaac aacgataaaa
       61 atgcttatat accaaacaaa aacgtggttg caaacaagtt gctagatcag ttgcgtgaag
      121 aaaatgaatt tttacgagaa caacttgccc tcaaagagca aaataattct catatacaaa
      181 aactactcga ccagcaacaa caactcaccc tgcaagccaa ccaacaaatc gaaaaacttc
      241 aggagcaact gcagttgact tatacggaag agtcttcaga gaatagttca acgactcttt
      301 ctgaagcaac agaaacaatg gacgaacaaa caaagaattg gaaaaaatgg tggcagctat
      361 ggaaatgaag aaggaaatac ttatgacaaa aaaacacttg ctattcggtt ctatcctact
      421 aggaatatta agctttagcc caatgttatt atcagtggcc aacaaaggaa ataaaaaagg
      481 ggaactaaaa ctcaatccta attactacaa cagtcaaaat gaggaagtat ttgtagaaaa
      541 acttgataaa taagaaaaga ctgctcgtac gagcagtctt tctgaaagag attatcctat
      601 tattaacctt agaaaagaat aataaatgaa tatgagatat gtaaaaagca taacaaacac
      661 aaacaaaaat aacaagtatt taaaaaatga ccgatcaata ttcacaaaga attagaaaga
      721 aacaaccgca gaaaaccaag ccttaaaaag agcttttaac ctagaccaat aaaaattcac
      781 aaaatcagcg cctggatccc ttggtacagg cgttacttgg tagtatgtac attatgtcta
      841 ctagctgttt aaataactga tcttataatt ttatttaaac gattatgaat tcaaaactac
      901 tttttatgac gttaatcatt gttatataat aaaatgttat ataataaaat tactaagatt
      961 atttaatata acaagcaatt ttaaaaaaac gaaaaggaaa aacaatcatg aaaaagaaag
     1021 tattaaaaca ttgtgttatt ctaggaatat taggaacttg tctagctggc atcggtacag
     1081 gaataaaagt tgatgcagct acttactatg gaaatggtct ttattgtaac aaagaaaaat
     1141 gttgggtaga ttggaatcaa gctaaaggag aaattggaaa aattattgtt aatggttggg
     1201 ttaatcatgg tccatgggca cctagaaggt agtggtaggg aggttattat aatggatttt
     1261 actaaagaag aaaaactttt aaatgcaatt agtaaagtat acaatgaagc aactatagat
     1321 gactatcctg acttaaaaga aaagctcttt ctttattcta aagaaatcag tgagggaaaa
     1381 agtgttggtg aagttagtat gaaattaagt agttttcttg gaagatatat tttaaaacat
     1441 aaatttggat tacctaaatc tttaatagaa ttacaagaaa ttgttagtaa ggaatctcaa
     1501 gtatatagag gatgggcttc tattggtatt tggagttaat tctttggaat aaaatgaaaa
     1561 tgggactgaa gaatcaattc tgattcatca gtcccataaa ttatagagcc gaaaaattat
     1621 catatttata gatttattaa tagtaaatgg cataagaatc tttgaacaac gaaaaaaatg
     1681 tagctctacc taattattga tttgcaacaa tttcaccgaa ggtgaaacaa gcgaaacttt
     1741 acaaaaagtg gctagcattt gctagtcttt tttgtattgt ggagagtggg gg
//
LOCUS       DAYYGY010000176         1738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000176 DAYYGY010000000
VERSION     DAYYGY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.182"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1026
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA52_002946"
                     /pseudo
     CDS             1..1026
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA52_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1010..1402
                     /locus_tag="ACHA52_002947"
     CDS             1010..1402
                     /locus_tag="ACHA52_002947"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8941577.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1403..>1738
                     /locus_tag="ACHA52_002948"
     CDS             1403..>1738
                     /locus_tag="ACHA52_002948"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8941578.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGI"
BASE COUNT          717 a          133 c          296 g          592 t
ORIGIN      
        1 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
       61 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      121 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      181 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      241 gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag tggttatgat
      301 agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag tactaataag
      361 aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact
      421 aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc tatactaggt
      481 tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat gtcagaagaa
      541 gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca cggtttagat
      601 tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga agagtatata
      661 ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta tatagaaagt
      721 tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt atgccataat
      781 gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg aataattgat
      841 tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact tgaagatagt
      901 gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg aaatatagat
      961 attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat tgaaactatt
     1021 gtttattgat gataatatgg tttttgatat tgataattta aaaggatttc ttaatgatac
     1081 cagttcattt gggtttatag ctaaagaaaa taataaaatt ataggatttg catattgcta
     1141 tacactttta agacctgatg gaaaaacaat gttttattta cactcaatag gaatgttacc
     1201 taactatcaa gacaaaggtt atggttcaaa attattatct tttattaagg aatattctaa
     1261 agagattggt tgttctgaaa tgtttttaat aactgataaa ggtaatccta gagcttgcca
     1321 tgtatatgaa aaattaggtg gtaaaaatga ttataaagat gaaatagtat atgtatatga
     1381 ttatgaaaaa ggtgataaat aaatgaatat agttgaaaat gaaatatgta taagaacttt
     1441 aatagatgat gattttcctt tgatgttaaa atggttaact gatgaaagag tattagaatt
     1501 ttatgatggt agagataaaa aatatacatt agaatcatta aaaaaacatt atacagagcc
     1561 ttgggaagat gaagttttta gagtaattat tgaatataac aatgttccta ttggatatgg
     1621 acaaatatat aaaatgtatg atgagttata tactgattat cattatccaa aaactgatga
     1681 gatagtctat ggtatggatc aatttatagg agagccaaat tattggagta aaggaatt
//
LOCUS       DAYYGY010000177         1691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000177 DAYYGY010000000
VERSION     DAYYGY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.183"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1636
                     /locus_tag="ACHA52_002949"
     CDS             158..1636
                     /locus_tag="ACHA52_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941579.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          614 a          232 c          302 g          543 t
ORIGIN      
        1 tttccatttt ttgcaatatt ctcaaaaaaa agataaaaaa tgagaataac tgatgaaaaa
       61 acaagcataa tccatcataa aatcagtata ggtgaaaaga tgaactttca tctgcatatc
      121 aagtaattgg tatgaggcat tttttgaggt gtaggatatg aagttggatt accttataga
      181 agaggaagat agagaaaaac tgctgctagc tagatattta gaatttgaga aaaagaatta
      241 tgtagtgctg cctaagttat atgaaacatt aggtttgtct aaatttaaaa caagaaatta
      301 tatcatagaa ttaaataaag aatttactga attcaaaaca aagccagaga tccaaatttc
      361 agaagaagca gagatccatg tcgaacgtct gaatttgacg gatatcaaaa atttaagaag
      421 aatctacttt caacgatcaa aaattgctcg actgttaacg gagatcgtgg attcaaaagt
      481 aacgatcgaa aaatttgcag agaatcattt tttaagcata tcaagagcct atgtaaaaag
      541 aagagaatta gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa acatattagt
      601 tggggaagaa ataaacatac ggaacgtatt atttggggta tatttcgggg tctttaatgg
      661 tttttctatg ccatttagag aaagcgtcat gaatgaagca aagagtatcg tgcaatattt
      721 tgaatatctg ttcaatttaa aactgtctgc aacaaaacga atcaaattca atttgctaac
      781 cactattagt ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga
      841 tgaatacttt aagagctcag aaggaatgaa gatcatcaag gatttatcac atatttcttt
      901 tcttcatatc cctaaagagc agatcattga tgagatttct tatatcatgt tgtttttgat
      961 cacggaagaa gaatttgaat tagatggcgc atttggactt gcggaagctc attttcatga
     1021 tatccacatg attagtcaaa aaatgtcagt ggaattgatt gatcaattac cattttcgat
     1081 tgaaatctct gaagaagaaa gactatatat acgaaaagaa cttcgtatgg gtatgatgca
     1141 tgtcaatcga aaaaattgga tattcgactt tgagatgact agctttacgt ctaaaaagca
     1201 gctacagttt ttcttcgaga cgtatccaat attcagtaca gcaatccaaa aggtcataca
     1261 agagaataaa cgctatttct tgggaaaaga tgaacgtttc atgattcgct tattttatga
     1321 ttatctcttt ttattggtcg gtatttgtcc ggtatcatat tttgaagcac cgattcatgt
     1381 ctgtattgat ttttcgcaag gatctgctta taccgattac attatttctc aagtgaaagg
     1441 atttaaaaat tacaatattg tgattgagag tcgaatcact tctcgtacga atatttattt
     1501 atctgattgt ttacttgaaa aatttaataa agaacagata atttggaaaa atccaccgac
     1561 tccagatgac tgggaatatt ttggaaatac catcatcaga gaaaaaaata gacatttaac
     1621 tgaaaaaatc agctagtatt tatggatggc agctttgtct cacatattta ttagctgatt
     1681 tatgtttttt t
//
LOCUS       DAYYGY010000178         1686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000178 DAYYGY010000000
VERSION     DAYYGY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.184"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(5..1486)
                     /locus_tag="ACHA52_002950"
     CDS             complement(5..1486)
                     /locus_tag="ACHA52_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941580.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPICFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          518 a          232 c          291 g          645 t
ORIGIN      
        1 tttgtcacat cctctttgtt ttgttctcat aacctttttt catatacagt tcatgaaccg
       61 cttttattaa cttgccaaat aacactgtat tgtgcgttgt atccagatag aaagtctccg
      121 tttcatctga aattttctca tgctgatctg taatgatcaa atcagcatgt tctgaatatg
      181 cggtaatttt aacagcatct ttgcgaaata actgctgcaa atggctttga agatgataat
      241 ttcctgtaaa ctgtttggtt atctgcaaat ggatgagtaa ctgctctttc gtttctgttt
      301 gtagtaagtg ttggatcaat tgactaaggt attgactgat ttggtttctt tccccatttt
      361 ttgaaaaaat tgtatcgatt ttttctctta atttcttttc ctgtataaaa gaactagaat
      421 gattggacgt ggcttctgga aagcaaagtg tagaaaaact actgctggat tcacccaaca
      481 gttcgaaaaa aacaactgcc aacacaagaa aataaaaaca aattggtatt ttattttcta
      541 aatggttgag aggaaactgt tgagttaagt aagtcatcaa ttgaccgact tgtcgacaaa
      601 taaggttttt gcttgtggaa aataaaaaac caatctcctg tttttgtcga aaagagattt
      661 gatttggaaa aaaacaatag aaaaaataat taaagtaggc tctctctgtt tctgtctctt
      721 cacaagggcg actcactgaa aaagaagtaa agtattctga ataatcatgt gtatgctgca
      781 ataagtccat caattcagtg atttcagaag aagtttttgg aaaatatgcc tgttgtttgt
      841 ttcgtaatat gaataaagtt aacagcaagt agatattttt ttcttgtttt gaaatttttt
      901 ctatttgctc atctgtactg ttcattttta gtgcatttaa ttcaagtgag gaaaatggcc
      961 aatcgttacc tccaaatgta tctgaaagaa aaaagtaaag atagagacgg atagataact
     1021 catttccttc aagagtacag caatttttta cagataggat ccgtaaatgg aattttttta
     1081 aggaatgatt gagctgtttt gtcagttttt cgagatagga gggcgatata taaagttgtt
     1141 caagtaaata tttttttggt acagcagttt gtggataatt gattagtaac atcaaatagt
     1201 taaaacgatt ggattctttt aggtagcata attttaactt agaaaaaaca gctaactttt
     1261 tagtttgatc ggtttctttg gtttggatct ctcctttatg attagaataa ataaattcat
     1321 caatcaataa aaattgcttg agtgatacat ttatttttcg gatcagttct ttgattgtcc
     1381 gttttggaat atgtaagtca ttggataatt cttgtaaaga acaattattt ttttgaatga
     1441 ccatttgtag aacaagtaac tctttttttc ttgcttcaat tagcataaat acctcctttt
     1501 tattctgttt ttgtatatag ttagacaaaa actgtgagtg gaaagtcatt tcagtctgtg
     1561 ttattctcac agagtgtcgc cttctttttt atataacgtt atttaatttt tttgttacct
     1621 tgtttattct attgtttatt tcctagggta ttttgtcaag atattcttgt ttttaaggcg
     1681 gaaaaa
//
LOCUS       DAYYGY010000179         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000179 DAYYGY010000000
VERSION     DAYYGY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.185"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA52_002951"
     CDS             131..505
                     /locus_tag="ACHA52_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8941581.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA52_002952"
     CDS             complement(619..1248)
                     /locus_tag="ACHA52_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8941582.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA52_002953"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA52_002953"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYGY010000180         1645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000180 DAYYGY010000000
VERSION     DAYYGY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.186"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1409
                     /locus_tag="ACHA52_002954"
     CDS             <1..1409
                     /locus_tag="ACHA52_002954"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8941583.1"
                     /translation="QTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAK
                     QATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDV
                     EVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSV
                     FDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNG
                     DTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLL
                     HGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPT
                     TVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLAN
                     FDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQT
                     TPENEAWLQGALEVFKHE"
     gene            1402..>1645
                     /locus_tag="ACHA52_002955"
     CDS             1402..>1645
                     /locus_tag="ACHA52_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8941584.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          575 a          276 c          347 g          447 t
ORIGIN      
        1 aacaaacgat gttgtatcgc gaattacttt cagatgaaga atcaaaagaa gatctgattg
       61 caacaatcgt tgatattttg gggtatgcga tttcgccaga gtatcttttc aacgtcttag
      121 cggatcaagc aaaacaagca acgttccaat tgaatgatct aaacaaagcc tttgtgcaat
      181 tggcatctac ctacaatcaa tttaatgggt tatttgatga tgtggatctc caatcaaaaa
      241 aactaggaac agatgagcaa caacgaaatg tgacgattac cgaagtcatt aagaaattga
      301 atgacgtgga agttttaggt cacgatgggg acgtgatcgg ggatgcttat gaatttctga
      361 tcagtcaatt tgcttcagaa gcagggaaga aagccggaga attttacacg cctcatatgg
      421 tttctgatat gatggcacaa atcgtgacgt tggatcaaaa agaacgacga ttctttagtg
      481 tttttgatcc aaccatgggt tcaggttcat tgatgttaaa tgtgcgtaac tacttaactc
      541 acccagataa cgttaaatat catgggcaag aattaaatac aacgacatac aatctagcaa
      601 aaatgaactt gattttacat ggggtagatg ccgaagaaat gaatctccgc aatggggata
      661 cgttgaataa ggattggcca acagatgaac cttacacatt tgatgcagta gtaatgaacc
      721 caccttattc ggctaattgg tcagcagata cgaccttctt agacgattct cggtttaatc
      781 gttatggaaa attagcgcca aaatccaaag cagattttgc gtttcttctt catggtttct
      841 atcatttgaa ggaaacaggg actatggcca tcgtcttgcc acacggagtt ttattccgtg
      901 gcgctgcaga aggggtcatt cgtcaaaaat tactagaaga cggcagtatt tatgctgtga
      961 tcggtatgcc tgcgaacttg ttcttcggga catcgattcc aacgacggtg attgtattga
     1021 aaaagaaccg ccaaactcga gacgtattat tcattgacgc tagtcgagaa tttgtcaaag
     1081 gaaaaaatca gaataaatta tcggaagaaa acattcaaaa aatcctcgaa acctatgctg
     1141 aaagaaaaga tgtcgagaaa tacgctcatt tggcaaactt tgatgaaatc aaagaaaatg
     1201 actataactt aaatattcca cgctatgtag atacgtttga agaagaagaa ccaattgata
     1261 tggttcatgt cggaaatgac atcaagaaga tacgacaaga acagcaagtg ctagaaaaag
     1321 aacttttaga agctatctct tccctgcaaa caacacctga aaatgaagcg tggttacaag
     1381 gtgcgctgga ggtgttcaaa catgagtaat gatatacaac cagaaattcg tttcccagga
     1441 tttacggaag cttgggaaca gcgtaagttt gaatgcttac ttgataaaaa agatggagta
     1501 aggaggggac catttgggag tgccttaaag aaagaattct ttgtatctaa tagcaatttt
     1561 gttgtctatg aacaacaaaa tgctatttat gataattacg aaacaagata taaaattact
     1621 gaaaaaaaat ataatgaatt aatca
//
LOCUS       DAYYGY010000181         1637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000181 DAYYGY010000000
VERSION     DAYYGY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.187"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..1593
                     /locus_tag="ACHA52_002956"
     CDS             127..1593
                     /locus_tag="ACHA52_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8941585.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          459 a          296 c          336 g          546 t
ORIGIN      
        1 ttgccattaa aaattacttg cttttataga aaattagttt atatttctta aataagtttt
       61 ttagttaaat ttttcggtaa agatgtataa tattttctga aaatttaaag aattggggta
      121 tagattatgg aaaaacagca agacaagggc ttcttcgggc agccgaaggg attgtcaact
      181 ttgttcttca ctgagatgtg ggaacgattt agttattacg gtatgcgtgc attacttatc
      241 tattatatgt acgatacagt tgccaacggg ggacttggat taccacgagc aacagcatta
      301 gctatcatgt cgatctacgg atctttgatc tacatgtcta gtatcgtcgg cggatgggta
      361 tctgaccgag tgttaggagc aaaacgcact gtattcttcg gtggagtttt cattatgctt
      421 ggccacatcg tcttggcttt accatttggt gtaagtacat tgtttgtttc gatggcattg
      481 attattatcg gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg acatctttat
      541 tcaaagactg acttgcgaag agatgcagga ttttcgattt tttacatggg tgtcaactta
      601 ggggcattat tagcaccact aatcgttggt acaatcggtc aagaatataa ctatcattta
      661 gggttctctt tagctgctat tgggatgttc tttggattat tacaatacat catccaagga
      721 cgcaagacat tagacggagt gggtacaaca ccgccgaatc ctttgacaac tgaagaacgt
      781 aaaggattca tccgtaactt gatcatcgca cttgttgtta tcgctgcagt attcggcggg
      841 gcacaactaa ctggtcatct gacaattgac ttttttgtca atacaatcag tgtattgggg
      901 attatcttac cgttgtatta ctttatcaaa atgctgactt caaaagatgt aactgctgaa
      961 gaaaaaccaa gagtatgggc ttatatccca ttattccttg cagcaatcat tttctggtca
     1021 ttacaagaac aaggttcatc tatcttagct ttatttgcta gtgaacgtac acaatctacg
     1081 ctatttggtg taacgatcca agcaagctgg tatcaatcat tgaatccaat tttcatcttt
     1141 attctgacac cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca accatctaca
     1201 gtagtgaaat tctctcttgg acttttattt gcaggattat cgtacttgct attgatgttg
     1261 ccgggtatgc tttatggtac aagtggaaaa gtaagtccat tgtggttgtt gggaagtttc
     1321 ttcatcgtgg ttattgctga attgtgtctt tcaccagttg gtttgtctgt gacaacaaaa
     1381 ttagcaccac gagcatttga atcacaaaca atcgctattt ggttcttagc tgatgcttct
     1441 tcacaagcaa tcaatgcaca aattgcacgc ttctacacac cgggaacaga atctgcttac
     1501 ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct tgctatttac tgtgaaagaa
     1561 ccaatcaaaa aactaatggg ctctattcat taatcaatga acataaaaac aggaattgct
     1621 tatttgcagc agttcct
//
LOCUS       DAYYGY010000182         1587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000182 DAYYGY010000000
VERSION     DAYYGY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.188"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..249
                     /locus_tag="ACHA52_002957"
     CDS             <1..249
                     /locus_tag="ACHA52_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941586.1"
                     /translation="TIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRN
                     EILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(426..554)
                     /locus_tag="ACHA52_002958"
     CDS             complement(426..554)
                     /locus_tag="ACHA52_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941587.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(611..850)
                     /locus_tag="ACHA52_002959"
     CDS             complement(611..850)
                     /locus_tag="ACHA52_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941588.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1136..1450
                     /locus_tag="ACHA52_002960"
     CDS             1136..1450
                     /locus_tag="ACHA52_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941589.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          542 a          218 c          278 g          549 t
ORIGIN      
        1 actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtgatt
       61 ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac
      121 gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt
      181 tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga acatacttta
      241 gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca
      301 ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga
      361 gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa
      421 ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt
      481 tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt
      541 tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa
      601 gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta
      661 ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct
      721 catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct
      781 ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa
      841 ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat
      901 taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc ttagtaatac
      961 atatatggag agggtgatat cccagttcaa accagataca ttggttacca tctgtattaa
     1021 cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac gtaggtatga
     1081 tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat agcttttgga
     1141 tttttacagt aattttatct taataatagc aatattatta cttctaaata tttggttttt
     1201 tgataagagt agaaatgcag ggataggttt tagaaccaaa cgaagtacgt cttctgaaaa
     1261 aaagtgggta tattcacaga ctatttttta cggaggggtt atttcaatca gtttactttc
     1321 ttcaacactt tattctttta atgttataga cgtttccatg tcaaatttta tatctattat
     1381 tggaatcctt atttctgcaa ttattacaca actacttctt gtgtttgaag agaagtcaaa
     1441 aaataattaa agcaattatg ccttttagta caggaattat tacttcttac aaatgtatag
     1501 cgaatgtgaa gcctgctgtt ttaattgttc tagaaaaact tttgagtgaa caattatttt
     1561 atgttaggat gaattaattt ctctaga
//
LOCUS       DAYYGY010000183         1565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000183 DAYYGY010000000
VERSION     DAYYGY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.189"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..454
                     /locus_tag="ACHA52_002961"
                     /pseudo
     CDS             1..454
                     /locus_tag="ACHA52_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            699..>1565
                     /locus_tag="ACHA52_002962"
     CDS             699..>1565
                     /locus_tag="ACHA52_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFM8941590.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNLD"
BASE COUNT          618 a          184 c          317 g          446 t
ORIGIN      
        1 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
       61 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
      121 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
      181 cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt taaagcgtga tgtgagttta
      241 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
      301 aacgttttta aggggtttgt agacaaggta aaggataaaa acagcacaat tccaagaaaa
      361 acacgattta gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa
      421 agaacgaagt cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt
      481 ctttttttga tggttttgaa cttgttcttt cttatcttga tacatataga aataacgtca
      541 tttttatttt agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat
      601 tgaaaaccct taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca
      661 aacaaataac taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt
      721 attcagatga aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat
      781 ggagagaaaa gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta
      841 aaaaggctga aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa
      901 caggcgaaag aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca
      961 actggaggag agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta
     1021 aacaaaagcc aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg
     1081 gcgaagaatt aaataagagt ttgtcagata tggctcaagg atttcgccga atgatgcaat
     1141 ataaaaaaat taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata
     1201 ataaagataa ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt
     1261 ttaagaatac agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa
     1321 tgaaattaga ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata
     1381 aatcggatat acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt
     1441 ttatgaccga tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac
     1501 accgtaaaag gttaatctcc tatggtggtt tgttaaaaga aatacataaa aaattaaacc
     1561 ttgat
//
LOCUS       DAYYGY010000184         1531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000184 DAYYGY010000000
VERSION     DAYYGY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.191"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..965
                     /locus_tag="ACHA52_002963"
     CDS             <1..965
                     /locus_tag="ACHA52_002963"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8941591.1"
                     /translation="NFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIF
                     NTFIPSVLFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCL
                     SQDTKEKLSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVA
                     KNLPVGWAIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFI
                     STSRSEGLPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKL
                     SNNRLLMEKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            1028..>1531
                     /locus_tag="ACHA52_002964"
     CDS             1028..>1531
                     /locus_tag="ACHA52_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFM8941592.1"
                     /translation="MIIRAINFYSLLPSTFDSIFFKLIGISGVLLLGIDFIIKFNKRK
                     VTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWAILQYFLIYQFAFENSDNKRFFK
                     RISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHRIRIGFLESRLFGTFSDINNAAI
                     ASLVVIVL"
BASE COUNT          561 a          152 c          269 g          549 t
ORIGIN      
        1 aaaatttttc tcgatttcat aagatattag agtatagatt gtttggaggc tttgttccta
       61 gtagatatat aaaaaaagaa aaagaacaac taaaaaaagt tctaaagaaa gaaaaacctg
      121 atgtcatcat attcaatact tttataccta gtgtactatt ctccgaattc ctaaagtatg
      181 aatttccaac agtgaaatta attacttgga tgcactctga tcctgattac agcttaaatc
      241 atattgctaa attttataaa aaagaatata gaaaaagttt tgaacgcgtg gattccattg
      301 tttgtctttc gcaagataca aaagaaaaac tttcattata caataaccat attaaagtta
      361 tttataaccc attgacatta agcggctcta gtttaataag taaccttgat aaaaaaatta
      421 tttcatttac tgccagatac gatataaata ttaaagggtt tgattattta tgtgaagtag
      481 ctaaaaactt acctgttgga tgggctataa ggattgctgg agatggtaca gaagaagaac
      541 gtaaagagct gaaaagatta attgagaaaa atggtgtgtt agataaaatt atttgcgcag
      601 gtgcattatt tggagatgaa ttaatcaatc attatataga aagttctatt ttcatatcta
      661 cttctagaag tgagggacta ccattagtgt tgatagaagc aatgacttat ggattacctg
      721 taataagttt tgatcatggt ggtgctaagg aaatattaca agatggaaat tatggaattt
      781 taattaagaa ttttgacgta caagatatga ataatgagat agaaaaattg tctaataatc
      841 gtttacttat ggaaaaatat caaaaattct ctttagaaag agctaaggat tttaatgaaa
      901 aaaaaataat tggtgaatgg caaacattgt taaatgaatt aggaaatgag gatgttagaa
      961 attgaggact tcgattaaaa attttaattt atggaaagaa tggtatctat atatattttt
     1021 tatagggatg attattcgtg caataaattt ttatagttta ttaccgtcca cttttgatag
     1081 tatttttttt aaacttatag gaatttcagg agttttgcta ttagggatag atttcataat
     1141 taaatttaat aaaagaaaag taacttataa cattttattg atagcatata tggtgatttt
     1201 agtaataacg agttttgttc ataaagaata tggtctagga gaaaacttaa agactataat
     1261 gtgggcaatc ttacagtatt ttttgattta tcagtttgct tttgagaata gcgataataa
     1321 acgatttttt aaaaggataa gttggggatt tatttatagt tggtttgttt tagtatcaat
     1381 gtcaatggtt cttttctttg gtaaattcgg ctatgagaaa tattataatg ctagacatag
     1441 gatcagaatt ggttttttag aatcaagact gtttgggacg ttttctgata ttaacaatgc
     1501 tgctatagca tctttagttg ttattgtcct a
//
LOCUS       DAYYGY010000185         1448 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000185 DAYYGY010000000
VERSION     DAYYGY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.194"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..802
                     /locus_tag="ACHA52_002965"
     CDS             131..802
                     /locus_tag="ACHA52_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8941593.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(962..1153)
                     /locus_tag="ACHA52_002966"
                     /pseudo
     CDS             complement(962..1153)
                     /locus_tag="ACHA52_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          530 a          215 c          196 g          507 t
ORIGIN      
        1 gaaacttatt ttttcttttt ttcatgtatt cttttatttt tataactagt gtattacttc
       61 aaaatttgat attatatggc atgaacagaa ttaaaatatc agttaaaata aaagttaagg
      121 agcattgtgt atgacaatta aaactagtct ccaaaatcaa gcttaccaat ctattcgccg
      181 acaaattatc tatgctgact tagaacctgg aaaaaaagta tcagaaaaaa aattagaaga
      241 aacactttct attggtcgta caccaatacg tgaagcactg attcaactcc gacaacaagg
      301 tcttgttgat acaattcctc aaagcggaaa ttatatctca ttaattgatt tagattcggc
      361 caaaaatgct cgatttgtca gagaacattt ggaacgacaa attatgttag agtgttgtgc
      421 caaaatgaat aaccaaaaaa aacggatttt agaaactata atttcagaac aagaaaaagc
      481 tgttagtcaa aaaaatgctc gtagtttttt tcataatgat aatttattcc ataaagcttg
      541 ttttgaaatt gcagagcgag aagaagtatg gcaatggtta gaaaataaca atacacactt
      601 agaacgattt cgatggctac gagtaaaaac ggaaggatta aagtgggaaa aaattatgga
      661 acaacattat caactatttg aggcattaac aaaacaagat ccagaagaag ctaactttct
      721 aacatcgttg catcttcatt taatgcttaa tgagcaaaat attgttttag ctactcatcc
      781 aaaatatttc aaaaatatat aacttttaac tctttttaaa ttaaatggtt gttctgatca
      841 gaaaaatatc gcttgttttc ttattatgta atatatttca taaactattc tgcataacaa
      901 ctattgtttg tactttttac attggataac taattatgac tgctgataac ttttgatatt
      961 aaactagtat ctccaataca aataaaaaat agatatcttg ttcatcaaaa ttagttcgtg
     1021 gatacttacc ttcatttaca tcattcagcg gtttcccatt ttcttttatt ctattatttc
     1081 attttctaat agacttggct tcatttcgca atactttttt tcaataggca tcaaattggt
     1141 gttgtttaga tatttagttg tagttttgtg acatgataag tatgctcctt tttctctttt
     1201 ccactaatac aacgatcaaa gggtgctttt ttcaaaaaac tagaaaaatc aaaataagat
     1261 tcgtattttg attctatata tcctgtttca actacatctt atatttcaca atagaataaa
     1321 tagccttttg catagctgct taacttataa gaaattgcaa tttacttctt caaagtgaaa
     1381 acgaatatga gtaaaataaa attattcttc ttacagataa ttaaaaatag cgcgtaacaa
     1441 aaatcggc
//
LOCUS       DAYYGY010000186         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000186 DAYYGY010000000
VERSION     DAYYGY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.195"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACHA52_002967"
     CDS             82..>1364
                     /locus_tag="ACHA52_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8941594.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAYYGY010000187         1280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000187 DAYYGY010000000
VERSION     DAYYGY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.197"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHA52_002968"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHA52_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHA52_002969"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHA52_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          452 a          214 c          175 g          439 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt
//
LOCUS       DAYYGY010000188         1250 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000188 DAYYGY010000000
VERSION     DAYYGY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.198"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..191
                     /locus_tag="ACHA52_002970"
     rRNA            <1..191
                     /locus_tag="ACHA52_002970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            286..401
                     /gene="rrf"
                     /locus_tag="ACHA52_002971"
     rRNA            286..401
                     /gene="rrf"
                     /locus_tag="ACHA52_002971"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            411..484
                     /locus_tag="ACHA52_002972"
     tRNA            411..484
                     /locus_tag="ACHA52_002972"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:445..447,aa:Asn,seq:gtt)
     gene            524..613
                     /locus_tag="ACHA52_002973"
     tRNA            524..613
                     /locus_tag="ACHA52_002973"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:558..560,aa:Ser,seq:gga)
     gene            628..699
                     /locus_tag="ACHA52_002974"
     tRNA            628..699
                     /locus_tag="ACHA52_002974"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:661..663,aa:Glu,seq:ttc)
     gene            715..787
                     /locus_tag="ACHA52_002975"
     tRNA            715..787
                     /locus_tag="ACHA52_002975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Val,seq:tac)
     gene            797..869
                     /locus_tag="ACHA52_002976"
     tRNA            797..869
                     /locus_tag="ACHA52_002976"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:830..832,aa:Asp,seq:gtc)
     gene            905..980
                     /locus_tag="ACHA52_002977"
     tRNA            905..980
                     /locus_tag="ACHA52_002977"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:938..940,aa:Phe,seq:gaa)
     gene            982..1062
                     /locus_tag="ACHA52_002978"
     tRNA            982..1062
                     /locus_tag="ACHA52_002978"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1016..1018,aa:Tyr,seq:gta)
     gene            1069..1142
                     /locus_tag="ACHA52_002979"
     tRNA            1069..1142
                     /locus_tag="ACHA52_002979"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Trp,seq:cca)
     gene            1145..1217
                     /locus_tag="ACHA52_002980"
     tRNA            1145..1217
                     /locus_tag="ACHA52_002980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1178..1180,aa:His,seq:gtg)
BASE COUNT          318 a          263 c          338 g          331 t
ORIGIN      
        1 cattgctggg tagctatgta gggaagggat aaacgctgaa agcatctaag tgtgaagccc
       61 acctcaagat gagatttccc atttctttaa gaaagtaaga tccctgagag atgatcaggt
      121 agataggtca ggagtggaag tacagtgatg tatggagcgg actgatacta atcgatcgag
      181 gacttaacca ataggttaaa agaaaagtac tcggaaaaaa acttatgtga cttcaaatcc
      241 agttttgagt gaacaaaatt tactcaataa aagataacac cacagtgtgg tggcgatagc
      301 gagaaggata cacctgtaac catgccgaac acagaagtta agcttcttag cgccgattgt
      361 agtgaggggt tgccccttgt gagagtagga cgtcgccacg ctgatgttat tccggcatag
      421 ctcagttggt agtagcgcat gactgttaat catgatgtcg taggttcgag tcctactgcc
      481 ggagttctca ttttgagaaa gagtattgag cttctttcac tctggagagt tgtccgagag
      541 gccgaaggag catgattgga aatcatgtag atggtgccaa actgtctcaa gggttcgaat
      601 cccttactct ccgtagcaaa tttacctggc ccgttggtca agcggttaag acaccgccct
      661 ttcacggcgg taacacgggt tcgaatcccg tacgggtcat tttaattgaa tgttggaggt
      721 ttagctcagc tgggagagca tctgccttac aagcagaggg tcagcggttc gatcccgtta
      781 acctccataa tgaactggtt ccgtggtgta ggggttaaca tgcctgcctg tcacgcagga
      841 gatcgcgggt tcgattcccg tcggaaccgt cgttcttgtt attcaattaa taattttaaa
      901 atatggcgcg gtagctcagt tggtagagca acggattgaa gctccgtgtg tcggcagttc
      961 gattctgtcc cgcgccacca tggaggagta gcgaagtggc taaacgcgac ggactgtaaa
     1021 tccgttcctt agggttcagt ggttcgaatc cactctcctc cattttatag gggcatagtt
     1081 taaaggtaga acagcggtct ccaaaaccgt tagtgtgggt tcaattcctg ctgcccctgc
     1141 catggcgaat gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc
     1201 gattcccatc attcgccctt tttattcttg gggtatagcc aagcggtaag
//
LOCUS       DAYYGY010000189         1205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000189 DAYYGY010000000
VERSION     DAYYGY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.199"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..408
                     /locus_tag="ACHA52_002981"
     CDS             <1..408
                     /locus_tag="ACHA52_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8941595.1"
                     /translation="IKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKI
                     LAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILI
                     GFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            422..778
                     /locus_tag="ACHA52_002982"
     CDS             422..778
                     /locus_tag="ACHA52_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8941596.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          432 a          176 c          225 g          372 t
ORIGIN      
        1 ataaaagtat atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa
       61 gcgaatcaac ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag
      121 atcaaaaaga tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg
      181 aaaatttacg aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc
      241 ttattcaaag aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata
      301 ctaatcggat ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt
      361 gccagaaatc tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt
      421 cgtggaagtg aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg
      481 gttagaacac catcaattgc cttatcaatt tgttgactta gaagaacgcg atttaagtac
      541 agaagaagca gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac
      601 ttggtctgag gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact
      661 gattttttta tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga
      721 agttctattt attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac
      781 tttaactgtt ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag
      841 catcaagcgt gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca
      901 atttatgtgg tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg
      961 tgtaaattta catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta
     1021 ttttaaaaaa catataaaat agttgttgac atgttagaaa tgacatgata agataactga
     1081 gttgttaaaa aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt
     1141 cttgacaaat aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg
     1201 aaata
//
LOCUS       DAYYGY010000190         1192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000190 DAYYGY010000000
VERSION     DAYYGY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.200"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHA52_002983"
     CDS             <1..1076
                     /locus_tag="ACHA52_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8941597.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DAYYGY010000191         1138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000191 DAYYGY010000000
VERSION     DAYYGY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.201"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1031
                     /locus_tag="ACHA52_002984"
                     /pseudo
     CDS             1..1031
                     /locus_tag="ACHA52_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          404 a          204 c          228 g          302 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
      661 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
      721 accaagctat tgatgaatta ttaacaactt ttgacatcaa tcgatcattg agtcataaag
      781 gctgtccttt tgataatgcc gtagctgaat caacttataa gtcgttgaaa gtagaatttg
      841 tctatcaata cacatttgaa accttacaac aattggattt ggagttattt gactatgtca
      901 attggtggaa ccaccttcgg ttgcacggta cacttggcta cgagacaccg gttggttacc
      961 gtaaccagag attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg
     1021 aggcagtcta actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat
     1081 tgtcaaggac aatcggaata gcatacggaa agaagttctg caccttataa aatttgtc
//
LOCUS       DAYYGY010000192         1058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000192 DAYYGY010000000
VERSION     DAYYGY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.202"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..992)
                     /locus_tag="ACHA52_002985"
     CDS             complement(36..992)
                     /locus_tag="ACHA52_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941598.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DAYYGY010000193         1033 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000193 DAYYGY010000000
VERSION     DAYYGY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.203"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(368..>1033)
                     /locus_tag="ACHA52_002986"
     CDS             complement(368..>1033)
                     /locus_tag="ACHA52_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8941599.1"
                     /translation="GVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNNLTILDTALE
                     NKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAK
                     DSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQIN
                     QMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEH
                     TIQ"
BASE COUNT          312 a          150 c          120 g          451 t
ORIGIN      
        1 gtctttatta taaatatgaa gtcaaaaaaa atcacctata ttttttactt aaaagaaaaa
       61 atatagaatt acaagtcgtt caaaagttta atcacctgtt attattacta actagtttcc
      121 atatgttatc atcataatta cttcttgata ataattcata ttacaaaatt attcggtacc
      181 ttttgtcaaa tcgaaatttt catatcaata ataagcgaga ataataaaaa gactactctt
      241 tcgagtagct atatactgat gtcatcaaaa atagaaaagg aaacggtgtt tagattgtat
      301 aatattatga catttatatt ctatctcatt atctccaaac ataccatttg ttaaattaaa
      361 actatatcta ttggattgta tgttcattta caatttgttg tgctttattt gcggtatcct
      421 ctacatcatt tttagctttt tgaagctttt caatgtaatt attcaaacta ctattttttg
      481 acttttcttc ttctagcttt tgctgagtag cttgcagttg attttgaatg ttattttttt
      541 cactcaacat ttgattaatt tgctggttta aactattaat agtagattgt tcttcattaa
      601 atttgttctt ttcattttgt aaacgattat tccaatcgct atttaatcga tttatttcag
      661 ctactttttg gttaatttcg tctattttcg cattaatttc attttgtttt ccttgcaatt
      721 cagagtcttt tgctgacaat tgattttcta aattagtttt ctctgatact aattgattct
      781 tgtcgttttc taaagatgcc actttttgtt tggaaacttc aatattagtt cttagttgtt
      841 caagagtttg gttgtttgaa gacaattgtg aatttaaaat actaattttt tgctctttat
      901 tttctagagc tgtgtctaat atagttaaat tgttgttgat cttttcaata ttatcttccc
      961 ctccccataa taaaatatta tcagcatatt gctttccgga gaaaattact aacatcaatc
     1021 ctataattac tcc
//
LOCUS       DAYYGY010000194         1021 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000194 DAYYGY010000000
VERSION     DAYYGY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.204"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACHA52_002987"
     CDS             2..460
                     /locus_tag="ACHA52_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8941600.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="ACHA52_002988"
     CDS             469..696
                     /locus_tag="ACHA52_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941601.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          166 c          180 g          356 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 t
//
LOCUS       DAYYGY010000195          999 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000195 DAYYGY010000000
VERSION     DAYYGY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.205"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(262..876)
                     /locus_tag="ACHA52_002989"
     CDS             complement(262..876)
                     /locus_tag="ACHA52_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8941602.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          321 a          204 c          154 g          320 t
ORIGIN      
        1 cttgtctctt tttccactac tttttatttt tctgacacta catattctta taactcattt
       61 ctcataactg tgaaacaact tataaaaata acaaacttta aacaaaacaa gcttaaaatc
      121 aaagatgatc catcttaaaa taactaaaac aaaaaaagta gtcgttgtta atcctcatta
      181 ataatcgttt tctttttttc aaacgctcca tttttatgtg gaacattatt tttttaagga
      241 actttgtcaa catgtggata actattttcc acttgtgtcc tggactagct gttttgctgt
      301 aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc
      361 tggatctaga tttggcaaag tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac
      421 caaatcagca aattcaaata atcgcaaacc actgctatgt tgcgatttga tacttcgtga
      481 caaatcaaat ccagtaacaa tgattaatgg atgaccattt tctttgatcc actgagctag
      541 ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga agaaaaatgt cttctgattt
      601 gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct agcgtttcat agacacctcc
      661 ggctggattt tcaatcaata tacttggtac aagatccggg gtttgatccc aaaattcaaa
      721 tgctacacca cttagtgtgc ggctagcata tatctgcaat ctaccacctt gattaatttg
      781 tttcgcgatc caagtgccag cttgaagcat cagcggctct tctatcctta atttctcttc
      841 caattggtga taatagtcaa aataacataa actcatttat ctctcccgcc tagctatact
      901 tgacatgaat agaaacagct gcctaatcta aggaagtaca taaaaaaaca gtagttagac
      961 agtaacacat gtggtctttg ctgtttcaaa cacaaaaaa
//
LOCUS       DAYYGY010000196          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000196 DAYYGY010000000
VERSION     DAYYGY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.206"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>986
                     /locus_tag="ACHA52_002990"
     CDS             22..>986
                     /locus_tag="ACHA52_002990"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8941603.1"
                     /translation="MSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITDSEYFIQF
                     IRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQ
                     QRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDL
                     DFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNL
                     GMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFYENFANIK
                     FPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYGY010000197          946 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000197 DAYYGY010000000
VERSION     DAYYGY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.207"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>946
                     /locus_tag="ACHA52_002991"
     CDS             <1..>946
                     /locus_tag="ACHA52_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HFM8941604.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYH
                     VEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSL
                     GYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGL"
BASE COUNT          323 a          151 c          202 g          270 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattagaact cgaacaagag gaaatcagaa gatttcagaa
       61 ttgctccttg taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct
      121 tatagaagtg ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg
      181 cggaaatgtc ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct
      241 ttatggcgga atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa
      301 aattacagaa atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc
      361 cggggaaatt gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac
      421 aaagctattg ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt
      481 tgaaccgagc aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga
      541 cagtgacccg cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt
      601 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
      661 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
      721 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
      781 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
      841 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
      901 tggttggaat gtgacggatt gtaaaatctg ttttaagtat ggctta
//
LOCUS       DAYYGY010000198          891 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000198 DAYYGY010000000
VERSION     DAYYGY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.208"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>891
                     /gene="bsh"
                     /locus_tag="ACHA52_002992"
     CDS             4..>891
                     /gene="bsh"
                     /locus_tag="ACHA52_002992"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8941605.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITA"
BASE COUNT          303 a          123 c          161 g          304 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc a
//
LOCUS       DAYYGY010000199          811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000199 DAYYGY010000000
VERSION     DAYYGY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.209"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACHA52_002993"
     CDS             <1..672
                     /locus_tag="ACHA52_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8941606.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAYYGY010000200          716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000200 DAYYGY010000000
VERSION     DAYYGY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.210"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..649)
                     /locus_tag="ACHA52_002994"
     CDS             complement(74..649)
                     /locus_tag="ACHA52_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8941607.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          240 a          153 c          125 g          198 t
ORIGIN      
        1 cttaatttga caaataataa aatgaaaaaa cagagccaga ttagcattat tttgctaatc
       61 cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgg ggcaacatag
      121 tgatgactaa tgaaacaacg acgatagaca acaagcgatc aaggtagtct gtacccgcct
      181 gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca
      241 tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga
      301 tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt
      361 tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg
      421 aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg
      481 tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga
      541 gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac
      601 acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc
      661 atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYGY010000201          709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000201 DAYYGY010000000
VERSION     DAYYGY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.211"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACHA52_002995"
     CDS             complement(<1..448)
                     /locus_tag="ACHA52_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM8941608.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          214 a          142 c          105 g          248 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tctatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaatcac acctataaat ttattacta
//
LOCUS       DAYYGY010000202          703 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000202 DAYYGY010000000
VERSION     DAYYGY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.212"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>703)
                     /locus_tag="ACHA52_002996"
     rRNA            complement(<1..>703)
                     /locus_tag="ACHA52_002996"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          150 a          226 c          163 g          164 t
ORIGIN      
        1 accagcggta agtccatccc ggtcctctcg tactaaggac agctcctctc aaatttccaa
       61 cgcccgcgac ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtgccg
      121 ctttaatggg cgaacagccc aacccttggg accgactaca gccccaggat gcgacgagcc
      181 gacatcgagg tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat
      241 ccccagggta gcttttatcc gttgagcgat ggcccttcca tgcggaacca ccggatcact
      301 aagcccgact ttcgtccctg ctcgacttgt tggtctcgca gtcaagctcc cttctgcctt
      361 tacactcttc gaatgatttc caaccattct gagggaacct ttgggcgcct ccgttacctt
      421 ttaggaggcg accgccccag tcaaactgcc catctgacac tgtctcccac cacgattagt
      481 ggtgcgggtt agagtggcca taacgcaggg gtagtatccc accagcgcct ccatcgaaac
      541 tagcgttccg atttctacgg ctcctaccta tcctgtacat gcggtacaga cactcaatat
      601 caaactacag taaagctcca tggggtcttt ccgtcctgtc gcgggtaacc tgcatcttca
      661 caggtactaa aatttcaccg agtctctcgt tgagacagtg ccc
//
LOCUS       DAYYGY010000203          646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000203 DAYYGY010000000
VERSION     DAYYGY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.213"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          196 a          144 c          115 g          191 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtacc cttctgtatc cgttgggaga gtaaggctca
      181 actgccccct aaaaagggtt ctaaaaaatc tttaaaaaat aaataaaata aacgcgcgcg
      241 cgcgagcaaa atttcccgcc aaaaaatcac ccacttttta aaataaacag gttcgcgcat
      301 gatattaaac tgccaatcta gctccctatg gtcgctgtaa gaaggagtga tcgctacgct
      361 ctcacatttt acttgcagca gtcgccttta ggctccttcg ccaaaaataa ttcattaggg
      421 actcctttgt ggcgtcctga gagctcgtct aagccgttct aagacaagat tcgataaaaa
      481 ctatgtcctt cgttctaaag cgccttacag gcggtcaggg caagccctaa caaccccaag
      541 gcaataaaaa acttaggctt atttttgctt ttttattgta taattgattg tcgcctatcc
      601 gacagtcttg cgactggaag gaggtgtgtt tcgtgttcac taactt
//
LOCUS       DAYYGY010000204          636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000204 DAYYGY010000000
VERSION     DAYYGY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.214"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>636)
                     /locus_tag="ACHA52_002997"
     rRNA            complement(<1..>636)
                     /locus_tag="ACHA52_002997"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          128 a          209 c          134 g          165 t
ORIGIN      
        1 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
       61 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      121 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      181 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      241 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      301 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      361 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      421 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      481 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      541 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      601 cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DAYYGY010000205          636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000205 DAYYGY010000000
VERSION     DAYYGY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.215"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACHA52_002998"
     CDS             complement(<1..448)
                     /locus_tag="ACHA52_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941609.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          186 a          116 c           87 g          247 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttat
//
LOCUS       DAYYGY010000206          631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000206 DAYYGY010000000
VERSION     DAYYGY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.216"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..488
                     /locus_tag="ACHA52_002999"
     CDS             <1..488
                     /locus_tag="ACHA52_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HFM8941610.1"
                     /translation="YRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAI
                     FKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEY
                     EEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLF
                     Q"
     gene            509..>631
                     /locus_tag="ACHA52_003000"
     CDS             509..>631
                     /locus_tag="ACHA52_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8941611.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERG"
BASE COUNT          281 a          114 c           98 g          138 t
ORIGIN      
        1 aatacagaac caaaggtttc gcatatttct ccaaagaaga ctttcttgaa ttattagata
       61 ttcctaaaag ttataagcaa cctgatatag ataaacgagt tataaaacct atacgtcaag
      121 aactaactgc aatcttcaaa ggactaacaa tcaaaaagaa atacggaaaa ggacggggaa
      181 aacctgttat tggctatcaa tttacgttca aacctgagat caaaaaagca gacgacttta
      241 acaaacatgc accaaccaaa gaagaccaaa ataaagcatt ggctttagct gataaacaaa
      301 aagaatacga agaacccgct acaacaatta acgaaaaaat caaacagaag aaacgagaac
      361 ttgaactcct ccaacaaatc aaacaactag aaaaagagaa aaaagctctt gaaaaacaaa
      421 cagagtccgc actttcggaa aaagagaaaa aaagtttatt atctgattta gataatttat
      481 tccagtagca acctaggagg caaccagtat gcaaccacaa gaaaccttta caataaagga
      541 attgtcagat ctttttaaga tgagtaggca agcaatatcg aaacatatcc aaaaattaga
      601 ttcttccatg atagctaaaa atgagcgtgg t
//
LOCUS       DAYYGY010000207          602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000207 DAYYGY010000000
VERSION     DAYYGY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.217"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(318..581)
                     /locus_tag="ACHA52_003001"
     CDS             complement(318..581)
                     /locus_tag="ACHA52_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HFM8941612.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          226 a          104 c           79 g          193 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tctatttttt
      601 ta
//
LOCUS       DAYYGY010000208          545 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000208 DAYYGY010000000
VERSION     DAYYGY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.218"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..>545
                     /locus_tag="ACHA52_003002"
     CDS             50..>545
                     /locus_tag="ACHA52_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8941613.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPR"
BASE COUNT          185 a           97 c          107 g          156 t
ORIGIN      
        1 gtatgattaa atcacgacaa aataaacaac tgagaggacg atatgctata tgaatgattc
       61 tatcaaaaaa atgctgcgat taatagagaa agatttgatg attacagagg tctcttacga
      121 gacctttcag aagaaaaaga cgttgatcgt cgatgctgtt ttctcacctg ctccccatac
      181 ctgtagaaat tgtggttcta ctgtggtaga tggaaacggg aaagtaattg tagtgaaaaa
      241 tgggaagaag gaaacgattg tccgctttga acaatacaat cacatgcctt tggttatgcg
      301 cttaaaaaag cagcgctata cctgtaaaaa ctgtcgaacc cattggacgg ctcaaagtta
      361 ttttgttcaa cccagacatt caatcgcgaa tcatgtcaga tataaaattg cttctttact
      421 gactgaaaaa gtatctttat cttttattgc gaaaaattgt caggtatctt tgaccaccgt
      481 tattcgtacg ttgaaagagt ttaaaagcta cttaccaaag caatctaaga ggattctccc
      541 cagag
//
LOCUS       DAYYGY010000209          536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000209 DAYYGY010000000
VERSION     DAYYGY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.219"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>536
                     /locus_tag="ACHA52_003003"
     CDS             51..>536
                     /locus_tag="ACHA52_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8941614.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YL"
BASE COUNT          194 a           84 c          108 g          150 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctg
//
LOCUS       DAYYGY010000210          531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000210 DAYYGY010000000
VERSION     DAYYGY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.220"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..455)
                     /locus_tag="ACHA52_003004"
     CDS             complement(<1..455)
                     /locus_tag="ACHA52_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941615.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKS"
BASE COUNT          150 a           88 c           84 g          209 t
ORIGIN      
        1 gatttgataa tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta
       61 ggaactttga tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta
      121 taactctgaa caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga
      181 aaaaaattct tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca
      241 atttctttca aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt
      301 ttagaagaat catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg
      361 tagaccttac ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga
      421 tagattctga atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg
      481 attgtttcct tgattttgga tatacttctt aatcacatca attggtgcgc c
//
LOCUS       DAYYGY010000211          528 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000211 DAYYGY010000000
VERSION     DAYYGY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.221"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACHA52_003005"
     CDS             143..400
                     /locus_tag="ACHA52_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941616.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          223 a           66 c           74 g          165 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt gttactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaag
//
LOCUS       DAYYGY010000212          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000212 DAYYGY010000000
VERSION     DAYYGY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.222"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA52_003006"
     CDS             complement(<1..132)
                     /locus_tag="ACHA52_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8941617.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAYYGY010000213          473 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000213 DAYYGY010000000
VERSION     DAYYGY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.223"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>473
                     /locus_tag="ACHA52_003007"
     CDS             <1..>473
                     /locus_tag="ACHA52_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFM8941618.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DAYYGY010000214          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000214 DAYYGY010000000
VERSION     DAYYGY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.224"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..>468)
                     /locus_tag="ACHA52_003008"
     CDS             complement(45..>468)
                     /locus_tag="ACHA52_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8941619.1"
                     /translation="DEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTA
                     LKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKE
                     NSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          166 a           71 c           24 g          207 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatca
//
LOCUS       DAYYGY010000215          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000215 DAYYGY010000000
VERSION     DAYYGY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.225"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          159 a           56 c           66 g          181 t
ORIGIN      
        1 caaattaaat atataaatta gttaatgtgt tgtgattgat tatttaatat agatataata
       61 caaaaaggca aagatatttt caaaacattt tttcttgatg tgatatcctt gtcttttttg
      121 taatgttttt aacttattgc ttttgcaaaa tttatttacc tgttttctgg atcaacgata
      181 gtgtatatta tgctatcctt gtttggattt taggacgttt aaagaggcac ttttttacat
      241 aacttaatcc atagaccttt taagcatgct gtgagataat aataaagaag agtttaaagc
      301 gcaccccaaa ccacttcccc ataagtgtat tacgctttaa actcttttat atttgaattc
      361 actaagaagc ataccatatt tttgaaaaaa agtgagaaaa aaggcttata attaggattt
      421 agagtaatta attagatgtt tattttatag gactgatact at
//
LOCUS       DAYYGY010000216          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000216 DAYYGY010000000
VERSION     DAYYGY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.226"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..>462)
                     /locus_tag="ACHA52_003009"
     CDS             complement(204..>462)
                     /locus_tag="ACHA52_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8941620.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          165 a           59 c           79 g          159 t
ORIGIN      
        1 gaattaaaaa tattaaacag gaatttatcg aaaatggtag aaaagaaatt tataaaagga
       61 cttataaaga ttgattatat aatatatgaa aagctattat aaaagacatt agtattaaat
      121 agtttaaaaa aatgaaaaat aataaaggaa gtgagtcaat tccagactcc tgtgtaaaat
      181 gctatacaat gtttttacca tttctactta tcaaaattga tgtattttct tgaagaataa
      241 atccattcat catgtaggtc cataagaacg gctccaatta agcgattggc tgatgtttga
      301 ttggggaaga tgcgaataat cttttctctt ctgcgtactt cttgattcag tcgttcaatt
      361 agattggtac tctttagtcg attgtgggaa tttccttgta cggtatattg aaaggcgtct
      421 tcgaatccat catccaatga tgcgcaagct tttgaatatt tt
//
LOCUS       DAYYGY010000217          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000217 DAYYGY010000000
VERSION     DAYYGY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.227"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(215..337)
                     /locus_tag="ACHA52_003010"
     CDS             complement(215..337)
                     /locus_tag="ACHA52_003010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941621.1"
                     /translation="MNMNRDVLKMNEFDQSLSYRNLQFRSIGLSINEKVKKNMF"
BASE COUNT          145 a           87 c           52 g          167 t
ORIGIN      
        1 aaaataaaaa aattattacg ttattaaaaa aacagatgaa agatcaggtt ttattagatc
       61 tcattctttc atcacttgaa gggatttaat gaactacttt ttcctctttg tcgtttttat
      121 atattcgtat tccttcaaac agtaggatag cctctttcaa tgcttgatat ttgaactgtc
      181 cgttacttaa aaatttagat gtgccgccct cttcctagaa catattcttt tttacttttt
      241 cattaattga cagaccaatc gaacgaaact gtaaatttcg ataggataat gattggtcaa
      301 actcattcat tttcaagaca tctcgattca tattcaagat aggaatcttt ttatcaacat
      361 cactttattc gtattttcct ctaccataaa agcttcacgc tccacaaaat tgttttatac
      421 actactattc agcccattca acgaccaatg a
//
LOCUS       DAYYGY010000218          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000218 DAYYGY010000000
VERSION     DAYYGY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.228"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHA52_003011"
     CDS             <1..>451
                     /locus_tag="ACHA52_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8941622.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYGY010000219          441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000219 DAYYGY010000000
VERSION     DAYYGY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.229"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>441
                     /locus_tag="ACHA52_003012"
     CDS             <1..>441
                     /locus_tag="ACHA52_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8941623.1"
                     /translation="TTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDT
                     EFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVD
                     VSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          179 a           67 c          101 g           94 t
ORIGIN      
        1 aacaacagtc tttgcagtga aagacggcgt aagcttacca gaagataaac ttccagtgtt
       61 gaaagcaaaa gatggttata cagatgcgaa atggccagag gaagcaactc aaccaattac
      121 agcagatgat acagaatttg tatcaagtgc aacaaaactg gatgataaat ctgacgctga
      181 caaatataat cctgaaggtc aaaaagtgac tacagaattg aataaggaac cagacgcatt
      241 tgagggaatt aagaacaaag aagatttacc aaaagatact aagtatactt ggaaagaaaa
      301 agtagatgtt agcgcagctg gaaataaaaa agggactgtt gtagtgacat atccagatgg
      361 atcatctgat gaagttgaag tagatgttac agtgacagac aatcgttctg acgccgataa
      421 atatgagcca acagtagaag g
//
LOCUS       DAYYGY010000220          425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000220 DAYYGY010000000
VERSION     DAYYGY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.230"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>425)
                     /locus_tag="ACHA52_003013"
     CDS             complement(<1..>425)
                     /locus_tag="ACHA52_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941624.1"
                     /translation="LTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIY
                     WVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTT
                     PGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          157 a           70 c           57 g          141 t
ORIGIN      
        1 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
       61 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      121 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      181 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      241 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt
      301 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      361 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      421 tcaat
//
LOCUS       DAYYGY010000221          401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000221 DAYYGY010000000
VERSION     DAYYGY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.231"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>401
                     /locus_tag="ACHA52_003014"
     rRNA            <1..>401
                     /locus_tag="ACHA52_003014"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          125 a           87 c          117 g           72 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt c
//
LOCUS       DAYYGY010000222          394 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000222 DAYYGY010000000
VERSION     DAYYGY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.232"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..394
                     /locus_tag="ACHA52_003015"
     CDS             <1..394
                     /locus_tag="ACHA52_003015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941625.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
BASE COUNT          159 a           40 c           69 g          126 t
ORIGIN      
        1 tatgttagat gatagattga cgtgttctaa ttgtggtaca ttattttact atgaggaagt
       61 tagaaatatt gtaaaacata gaaaaaaaga aatgcctata cgttgtccaa attgcaatga
      121 agttattaag cgaaagttgt ctcatggtta ttttgtcagc tacgaactat atgaagaaag
      181 taaaataaat ctgaaaaaaa aaccaaaata ctataaaaat ttgaaaactg gagctattat
      241 aactgaaaaa gaatttaaaa atttagtgac tgaagaagaa agcaggtttt ataatttgtc
      301 tggtaatatt tgccctggat ttatggatca gattattgaa aatgagtcaa ttgaatcaat
      361 tgaaaactat gttcctgttg acttaccaaa atag
//
LOCUS       DAYYGY010000223          349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000223 DAYYGY010000000
VERSION     DAYYGY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.235"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>349
                     /locus_tag="ACHA52_003016"
     rRNA            <1..>349
                     /locus_tag="ACHA52_003016"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          116 a           67 c           97 g           69 t
ORIGIN      
        1 cagagatttc cgaatggggg aacccaacat ctttaatagg atgttacgat tgtgtgaata
       61 catagcacat tcgaggtaga cgcagagaac tgaaacatct aagtacctgc aggaagagaa
      121 agaaaattcg attccctgag tagcggcgag cgaaacggga aaagcccaaa ccagcaagct
      181 tgcttgttgg ggttgtagga ctccaatatg gtagttcttt cagatagtcg aatgacttgg
      241 aaaagtcagt caaagagggt aaaaaccccg tagacgaaat gtggaagaca cctaggagga
      301 tcctgagtac ggcggaacac gagaaattcc gtcggaatcc gggaggacc
//
LOCUS       DAYYGY010000224          348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000224 DAYYGY010000000
VERSION     DAYYGY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.236"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA52_003017"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA52_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8941626.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          110 a           52 c           56 g          130 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaaca
//
LOCUS       DAYYGY010000225          324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000225 DAYYGY010000000
VERSION     DAYYGY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.237"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>324
                     /locus_tag="ACHA52_003018"
     rRNA            <1..>324
                     /locus_tag="ACHA52_003018"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           98 a           62 c           94 g           70 t
ORIGIN      
        1 gaacccacgt acgttgaaaa gtgcggggat gaggtgtggg tagcggagaa attccaaacg
       61 aacttggaga tagctggttc tctccgaaat agctttaggg ctagcctcgg aattgagaat
      121 gatggaggta gagcactgtt tggactaggg gcccatctcg ggttaccgaa ttcagataaa
      181 ctccgaatgc cattcattca tatccgggag tcagactgtg agtgataaga tccatagtcg
      241 aaagggaaac agcccagacc accagctaag gtcccaaaat atatgttaag tggaaaagga
      301 tgtggggttg cacagacaac tagg
//
LOCUS       DAYYGY010000226          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000226 DAYYGY010000000
VERSION     DAYYGY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.238"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHA52_003019"
     CDS             complement(<1..>321)
                     /locus_tag="ACHA52_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8941627.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYGY010000227          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000227 DAYYGY010000000
VERSION     DAYYGY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.239"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHA52_003020"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHA52_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYGY010000228          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000228 DAYYGY010000000
VERSION     DAYYGY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.denovo.240"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACHA52_003021"
     CDS             <1..>299
                     /locus_tag="ACHA52_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8941628.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAYYGY010000229        26678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.034, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000229 DAYYGY010000000
VERSION     DAYYGY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.034"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..543
                     /locus_tag="ACHA52_003022"
     CDS             <1..543
                     /locus_tag="ACHA52_003022"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8941629.1"
                     /translation="EIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEI
                     SENHRILASLPIETYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKM
                     HGDIDDVSSTVITRDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSD
                     IRWVLRENQRPHYCIMRKIL"
     gene            1031..1996
                     /locus_tag="ACHA52_003023"
     CDS             1031..1996
                     /locus_tag="ACHA52_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8941630.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            2018..2230
                     /locus_tag="ACHA52_003024"
     CDS             2018..2230
                     /locus_tag="ACHA52_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8941631.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            2305..2781
                     /locus_tag="ACHA52_003025"
     CDS             2305..2781
                     /locus_tag="ACHA52_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8941632.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2778..4586
                     /locus_tag="ACHA52_003026"
     CDS             2778..4586
                     /locus_tag="ACHA52_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8941633.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4663..4878
                     /locus_tag="ACHA52_003027"
     CDS             4663..4878
                     /locus_tag="ACHA52_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8941634.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            4970..5833
                     /locus_tag="ACHA52_003028"
     CDS             4970..5833
                     /locus_tag="ACHA52_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8941635.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            5870..6406
                     /locus_tag="ACHA52_003029"
     CDS             5870..6406
                     /locus_tag="ACHA52_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8941636.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            6420..6842
                     /locus_tag="ACHA52_003030"
     CDS             6420..6842
                     /locus_tag="ACHA52_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8941637.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6772..9171
                     /locus_tag="ACHA52_003031"
     CDS             6772..9171
                     /locus_tag="ACHA52_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8941638.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            9203..11194
                     /locus_tag="ACHA52_003032"
     CDS             9203..11194
                     /locus_tag="ACHA52_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8941639.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            11217..11468
                     /locus_tag="ACHA52_003033"
     CDS             11217..11468
                     /locus_tag="ACHA52_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8941640.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            11458..12687
                     /locus_tag="ACHA52_003034"
     CDS             11458..12687
                     /locus_tag="ACHA52_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8941641.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12684..14765
                     /locus_tag="ACHA52_003035"
     CDS             12684..14765
                     /locus_tag="ACHA52_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8941642.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            14914..18834
                     /locus_tag="ACHA52_003036"
     CDS             14914..18834
                     /locus_tag="ACHA52_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8941643.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            18835..19779
                     /locus_tag="ACHA52_003037"
     CDS             18835..19779
                     /locus_tag="ACHA52_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941644.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            19783..19986
                     /locus_tag="ACHA52_003038"
     CDS             19783..19986
                     /locus_tag="ACHA52_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8941645.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(20286..20522)
                     /locus_tag="ACHA52_003039"
     CDS             complement(20286..20522)
                     /locus_tag="ACHA52_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8941646.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20705..21076)
                     /locus_tag="ACHA52_003040"
     CDS             complement(20705..21076)
                     /locus_tag="ACHA52_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8941647.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(21073..22401)
                     /locus_tag="ACHA52_003041"
     CDS             complement(21073..22401)
                     /locus_tag="ACHA52_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8941648.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(22362..22691)
                     /gene="mobC"
                     /locus_tag="ACHA52_003042"
     CDS             complement(22362..22691)
                     /gene="mobC"
                     /locus_tag="ACHA52_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8941649.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(22949..23319)
                     /locus_tag="ACHA52_003043"
                     /pseudo
     CDS             complement(22949..23319)
                     /locus_tag="ACHA52_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            23464..23649
                     /locus_tag="ACHA52_003044"
     CDS             23464..23649
                     /locus_tag="ACHA52_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8941650.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            23807..23974
                     /locus_tag="ACHA52_003045"
     CDS             23807..23974
                     /locus_tag="ACHA52_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941651.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            24206..24379
                     /locus_tag="ACHA52_003046"
     CDS             24206..24379
                     /locus_tag="ACHA52_003046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941652.1"
                     /translation="MKSHTARCIRKKEAAHFAEAWGVPNKQNENAAGGQVVDQNIRKG
                     YSDPLLASIIGMF"
     gene            24592..25251
                     /gene="vanR-B"
                     /locus_tag="ACHA52_003047"
     CDS             24592..25251
                     /gene="vanR-B"
                     /locus_tag="ACHA52_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8941653.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            25254..26615
                     /gene="vanS-B"
                     /locus_tag="ACHA52_003048"
     CDS             25254..26615
                     /gene="vanS-B"
                     /locus_tag="ACHA52_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8941654.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         6666 a         7019 c         7258 g         5735 t
ORIGIN      
        1 gaaatcgggt tggattctaa aaaagaaaca gacttagtta ctctggctca atattataaa
       61 aatgaaaatg gtggatcaag aggaattttg aatcagatac ttatggataa ttttggtgaa
      121 gaattagaaa tatctgaaaa tcatcgaata ctagcaagtt tacctattga aacttattgg
      181 acaactaatt atgatcatct aatagaaaaa tcaatccgag aagcttataa aaaccctcag
      241 gttaagaaaa attatactca gttagcaaca actaatccaa atgtggatac aattgtttat
      301 aaaatgcatg gtgatataga tgatgtcagt tcgacagtaa taacaagaga tgattatgaa
      361 aaatatgatg attcgtacgc gttatttaaa gaaactttaa aaggagattt gttaacaaaa
      421 acttttctct ttttaggatt tagttttaca gaccctaatt tagaaagaat tttatcagat
      481 ataagatggg ttttgagaga aaaccaaaga cctcattatt gtataatgag aaaaatatta
      541 tagaaaaaat tttaggttat cgctgaaagc cccggaaata cgggcatttc gcaggatatg
      601 agaactcaat ttactaccaa tttactactt tcggattgag ccgcatatat gctgaaccat
      661 actgctaata cccgccaccc caaatacggc acatcataaa ttgaatacga caccgtaaac
      721 gcggcgttcc tctttcccgt catgggagaa caggacgccg cttttttatg cccaaataga
      781 gaaaggaggc gacgccatgc tatttacttc cggccctgac cgggcgtttg aacggctgat
      841 gcaggaaaag cccggtttcg atcactacga cggcggcggg gcggacgcgc cggaggattg
      901 cggcacctgc cgattttacc gcccggattg gaaatatcaa ttctgccagt ttgcagagtg
      961 tccctatcag ccgggcaagc tgaccgacta tgcagcggtc aaattcacag tgaaaggagc
     1021 gaacaacgct atggcagttt tccgtgtgga aaagaacaaa ggctatacgg tgatgtcaaa
     1081 tcaccacctg cggaataaga acctgacctt aaaggccaag ggcctgctgt cgcagatgtt
     1141 gtccttgccg gaggattggg actataccct aaaaggactg tcctatatca accgggaaag
     1201 catcgacgcg atcaggacag cggtatggga gcttgaaaaa gctggatata tccggcgtga
     1261 gcagggccgt gacgcaaaag gcaaaatggc cgatatggtc tataccatct acgaacagcc
     1321 ggtattggaa aacccggtat tggaaaatcc aacatcggat aacccggtgt tggaaaatcc
     1381 gacatcggat aatccggcgt cggaaaatcc aacgcaatta aataaagaag tacaaagaac
     1441 tgacttatca aaaaaagaaa aaataattac agatggacaa agtacccatt ccattcctat
     1501 cccatccccg acccctgccc ctatggaggg cgaggcagcg gctacgccgc cggaacggaa
     1561 aaggacagga aaggatgagg ctgtacggat ttatcgggaa atcattttgg agaacatcga
     1621 gtatgcctat ctcattcagg acaggagcat tgaccgtgaa cagcttgacg aaatcgtaga
     1681 cctgatgctg gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg gggacgacta
     1741 ccctgccgag ctggtgaaat ccaagtacat gaagctgacc ggcgaacata tccgctttgt
     1801 ccttgactgt ctgcgggaga acacaacgaa agtccgcaat atcaaacaat acctgcgggc
     1861 tatgctgttc aacgccccca gcaccatttc caactactac acggcccttg tcgcgcacga
     1921 catggcacag cccgattggg gaaagcctaa atccggcctg ccggactatt cctgcggcga
     1981 gggcgaaagc ctgtaacgag aaaaggagga cgaccctatg aagcagggcg cattgatttt
     2041 tgacgagcga acagaccgtt acgacatccg ctttgatctg gccgactatt acggcggcct
     2101 gcattgcggg gagtgtttcg acgtgatgat cggcggcaaa tggaggccca cccgcattga
     2161 aatggccgaa aactggtatc ttgtcggcat ccgggccgac gacctttccg gcctgcgggt
     2221 gcggatttga cctatatcta cctgcggcgt ctggtggttc cgccttagac gccgcttttg
     2281 actttctgac aaggagggat gcgattgcag gaagaagtta ctcaaaagac cattgccctg
     2341 tccatgaaaa cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa aaagtacctg
     2401 caacaccggg ccaagggcaa aaacacgctg caccacggcc agcagagcct aaagcagctt
     2461 aaaaagcatg gggctgatct ctccaacatt gagatcaccg aggccaacat tggagcgttc
     2521 aagccatgcg ccaagaaata cggcgtggat tttaccttac gaaaagataa gaccacccag
     2581 ccgccccact acgttgtgat tttcaaggcc aaggatgcag ataaccttga tctggcgttt
     2641 cgggagttta ccgccaagac gctttccaag gagcagcggc cctctatccg caaggttttg
     2701 tccgctgtga agcaaaaggc ggcggcccag accaagcagc gggccaagga gaaaatcaag
     2761 gaaagggggc tggaactgtg aagcctgacg taaagaaatt gctgatcctg aacctcccgt
     2821 atctgatttt tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg gccgctggtg
     2881 ctgacctgtc ggggaagctc ctgcattttg cggatggttt tgcggcggct tttgccaacc
     2941 ctctgccaag cctgcacctg tttgacctgt gtatcggtat cgccggtgcc gtgatcgtgc
     3001 ggctggtggt gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc atggagtatg
     3061 gcagcgcccg ctgggggacg gccaaagaca tagcgcctta catcgacccc aagtttgaaa
     3121 ataatatcct gctcacccaa acggaacggc ttaccatgac cggcaggccc aaagacccca
     3181 agacagcgcg gaataaaaac gtgctggtga tcggtggcag cggttcaggg aaaacgagat
     3241 ttttcgtgaa acccaacctg atgcaatgct ttccgaccac ggactatccg acttcgtttg
     3301 tggtgacaga tccgaaaggc actttggttc tggaaaccgg caggatgttc cagcaggcgg
     3361 gctaccgcat taaaatcctg aataccatta acttttccaa gtccatgaag tacaacccct
     3421 ttgtctacat ccattcggaa aaggacgtgc taaagctggt aaataccctg atttccaaca
     3481 ccaagggcga gggggaaaag tcagcggagg atttttgggt gaagtcggaa cggttgtttt
     3541 attcggccct gatcggctat atctggtacg aggccccagc ggaggaaatg aacttctcaa
     3601 cgctgctgga aatgataaac gccagtgaag cccgcgagga cgacccggag tttcaaagcc
     3661 ccgtcgatct catgtttgag cgtttggagc agaaagaccc cgaccatttt gcggtgcgcc
     3721 agtacaagaa attcctgctg tccgcaggca agacccgttc ttccatcctg atttcatgcg
     3781 gtgccagact tgcgcccttt gacatccggg aggtacgcga gctgatggag gacgacgaaa
     3841 tggagctgga caccatcggg gacgaaaaga cggtgctgta tctcattatg agcgatacgg
     3901 acacgacctt taatttcatt ctggctatgc tccaaagcca gctaatcaac ctgctttgtg
     3961 accgggctga tgatgtgtac ggcgggcggc tgcctgtcca tgtacgcctg attttggatg
     4021 aatttgccaa cattggccaa atacccaatt tcgataaact cattgccacc attcgaagcc
     4081 gtgaaatatc ggcctctatt attttgcaga gccagtcgca gctaaaagcg atttacaagg
     4141 acgccgccga gatcatttcg gataactgcg atagtgtgct tttccttagt gggcgcggca
     4201 aaaatgccaa ggagatttcc gatgcgctgg ggaaagaaac gattgacagt ttcaacacca
     4261 gcgagaaccg gggaagccaa acgtcccacg gattgaacta tcaaaaatta ggaaaggagt
     4321 tgatgtccca agacgaaatt gccgtgatgg acggcggcaa gtgtatcttg cagctacggg
     4381 gtgtgcgccc gtttttatcg gagaaattcg atattaccaa gcatccccgg tacaaatacc
     4441 ttgccgacgc cgacaagaag aatacctttg acgtggacag atttttgact atcacgcgcc
     4501 ggaaacggca gcaagtggta acgcaggatg aagtattcga cctgtatgaa atcgacctgt
     4561 cggacgagga cgccgccgct gaataaacag cggctttttt cattccaaca acaaaatcaa
     4621 aaaatcatta tgagccggaa accggcagaa agaggtaaat ttatggcatt ttttaattcc
     4681 gcagttacta ctttgcagac tatcgtgatc ggactgggcg gcgctctgtg cgtatggggc
     4741 ggcatcaatc tgttggaggg atacgggcag gacaatccgg gggcaaaatc gcagggtgtc
     4801 aagcagcttg tcgctggcgg cggcgtggcc ctgatcggtg tcactctggt tcccctgctg
     4861 tccgggctgc tgggctgatc ctggcctttg tccctgtcca aacccttaac taatcgcctc
     4921 cgcgtcctcc cggccagacc gggagggcgc gggaaaggag gtgctacccg tgattagtga
     4981 aatcattgaa gaatggataa aaggcatcct catggacggt atcacgggca acctgtccgg
     5041 gctgttcgat acggtaaatg ccaaagtcgg agaaatcgcc gccgacgtgg gacgaacccc
     5101 gcaggcatgg aacagcggta ttttcaatat gctgcacagc ctgtcggaaa ccgtgatcgt
     5161 ccccatagcc ggggccatac tcgcccttgt catgtgctat gagcttatcc agatgataac
     5221 ggaaaagaac aatatgcacg actttgacag ctccatgttc ttccgctgga tattcaaatc
     5281 cgcgtttgca atcctgatcg taacgaacac atggaacatc gtcatgggcg tattcgacgc
     5341 cacccaaagc gtcgtcaatc aaagcgccgg tgtgattatc gggcagacat ccattgattt
     5401 taacaccctg ataccagacc ttgaaagcca attggaggct atgagcgtgg gcgctctttt
     5461 ggggctgtgg ttccaaaccc ttgttgtggg cctcaccatg aatatccttt ccatctgcat
     5521 tttccttgtg acctatggcc gcatggtgga aatctatgtt gtcacgtcgc tggggcctat
     5581 cccccttgcc acaatgggca acgcggaatg gcgcggcatg ggccaagggt atctgaaatc
     5641 cctgctggcg ctgggcttcc aagccttttt aatcatggtt gtggtgggta tctacgcggt
     5701 gctgatacag aatatcggtt ccgcttccga catatccggc gcaatctggg gctgtatggg
     5761 ctatacggtg ctgctgtgct tctgcctgtt caagaccggc agtatatcaa aggccgtatt
     5821 cggggcgcat tgatcccaaa atccaagcat acacaggagg aacttgttta tttcaacgta
     5881 caacatcatt tacgctgacc cgccgtggcg gtatgactgc aaaaacgtac agggcgcagc
     5941 ggaaaatcac tattccacca tgagcattga cgagctgtgc gccctgccgg tggaacggct
     6001 ggcgtccaaa gactgcctgc tgtttttgtg ggccacattc cccatgctgc cggaagctct
     6061 gcggttaatc aaagcgtggg gcttttcatt caagaccgtg gcttttgtat ggctgaaaca
     6121 gaaccgtaaa agccttacat ggttttacgg gctgggccgc tggacaaggg gaaacgcgga
     6181 aatctgcctg ctggcaacac acggcaagcc gaagcgtcgt tctgcctctg tccatcagtt
     6241 tattatcagc ccgattgaac aacacagcaa aaagccggat gtgacccgtg aaaaaatcgt
     6301 agagctggcg ggcgatttgc cccgcgtgga gctgtttgcc agacagaaaa cccccggctg
     6361 ggacgtgtgg gggaacgagg tggaatgtga ccttatcctg acgtgaaagg aggtgccaaa
     6421 tggcttatgt aaccgtcccc aaggacttaa cccatgtgaa aagcaaggtt gtgtttgggc
     6481 ttaccaaacg gcaactgatc tgtttttccg gggcgctggt gatcggcgtc cccttatatt
     6541 ttctcacccg tgactatctt accaacagcg cggcggccct gctcatggta tttgccatgc
     6601 tgccgggctt tttgcttgcc atgtacgagc gtcacggcca gccccttgaa gtggtgatta
     6661 tgcagatcat ccagtgctgc ttcatccagc caaaggagcg tccctatcag accgacaaca
     6721 cctacaccgc ccttgtgcgg caataccaga tggaacagga ggtaaaggcc attgtccaaa
     6781 aaggaaatcc caagagaaac cgaaaaaaca aagctgaccc gcgcccagcg aaaagaaatt
     6841 gacgcggtaa tccgtaaata taagggcgac ggcaggcccc acacggcgca gcagagcatc
     6901 ccctacgagg tcatgtaccc ggacggcgtg tgccgggtat cgcccggcgt cttttccaag
     6961 tgcattgagt ttgcggacat cagctatcag cttgcccagc cggacacgca gacggctatc
     7021 tttgaaaaac tgtgcgacct gtataactat gtggacgcct ccattcatat ccagttttct
     7081 ttcctcaatc ggaaagttga cccggtgcag tacgccaaga gctttgagat cgcgccgcag
     7141 ggggacgact tcgacgacat ccgggccgag tacaccggca ttctgcaaaa gcagcttgca
     7201 aacggcaaca acggcatggt aaagaccaaa tacctgacat tcaccatcga ggccgaaagc
     7261 gtgaaagcgg cgcgagccag attgaagcgc atcggctttg acctgttagg ctatttcaag
     7321 agcatggggg ccgtagccca tgtgatggac ggctgggaac ggctgaacct gctgcatggc
     7381 gtgtaccacc cggacggcga gatattcaat tttgactgga agtggctggc cccgtccggc
     7441 ctctccacca aggattttat tgccccgtcc tccctgtgct tcggcaatgc aaagacattc
     7501 ggcatgggcg gcaaatacgg cgcggtaagt ttcctgcaaa tcctgtcccc ggagctgtcg
     7561 gacgatatgc tggccgactt cctcaatacg gaaagcggtg ttttagtcaa tctccatgtg
     7621 caggccattg aacagaccaa ggcgatcaag accatcaagc gcaaaattac cgaccttgac
     7681 gctatgaaga ttgcggagca gaaaaaggcc gtccgcagcg gttacgatat ggacattctt
     7741 cccagcgacc ttgccaccta cggcgaggac gccaagaagc tgctgacaaa gttgcagacc
     7801 cgcaatgaac ggctgttcca gttgacgttc cttgtcctca acgtggccga caccaagcaa
     7861 aagctgaata acgacgtgtt ccaagccgca ggcgtggccc aaaagcataa ctgtcccctt
     7921 gtccggctgg actaccagca ggaacagggc cttgcaagca gcctgccgct gggcgtcaat
     7981 cagatcaaga tacagcgcag ccttacgact tccagcgtgg ccgtgttcgt cccctttgtg
     8041 acgcaggagc tttttcaggg cggcgcggcc atgtattacg gcatcaacgc caagtcccgc
     8101 aacatgatta tgctggacag gaagcaggcc cgttgcccca atgccttgaa gctcggcacc
     8161 cccggcagcg gcaaatccat gagctgtaaa tccgagatcg tcagtgtgtt cttaacgacg
     8221 cctgacgata tttttatttc agacccggag gccgaatatt acccccttgt caaacggctc
     8281 cacggacagg taatccggct gtcgcctacc agcaaggact ttgtgaaccc gctggacatc
     8341 aacctgaatt attcggagga cgataacccg ctggccctga aatccgactt tgtgctgtcg
     8401 ttctgtgagc ttgtcatggg cggcaagaac gggctggaag ccattgaaaa gaccgtgata
     8461 gaccgggctg tgcgcgtgat ctaccggccc tatctggcag acccccgccc ggagaatatg
     8521 ccgatcctgt ccgacctcca caaagcgttg ttagatcagc atgtcccgga ggctgaccgg
     8581 gtggcgcagg cccttgacct gtatgtgtcc ggctccctga acgtatttaa ccaccgtacc
     8641 aacgtggaca tcggcaaccg gcttgtgtcc tttgacatta aggagctggg caagcagctc
     8701 aaaaagctgg ggatgctcat tgtccaagac cagatatggg ggcgcgtcac ggccaaccga
     8761 agccaaggaa aggccacatg gtattttgcc gacgagttcc atttgctttt gaaagaggaa
     8821 cagacggcgg cctattctgc ggagatttgg aagcggttta ggaaatgggg cggcattcca
     8881 acaggtgcca cccagaacgt caaagacctg ctttcctccc cggaaattga aaatatcctt
     8941 gaaaacagcg acttcattac gctgctcaat caggcgtccg gggatagaaa aatccttgcg
     9001 gaacgcttga atttgtccac cgaacagcag aagtacatcg acaattccga acccggcgag
     9061 ggccttttga tctttgaaaa cgtggtgctg ccctttacca accccatccc gcacaacacc
     9121 cagctttaca agattatgac gacccgctta aacgaggtgg cgggagtatg acccggcgac
     9181 gccacaaagg aggtgaacca ccatgaaaga gctgaaagcc aaggcgaaag tgacgcaggc
     9241 catgacccgc gacgggctga cgctggaaaa tcaggccacc ggcgaggtgg agaacatttc
     9301 cagccgggag gccgaacagg atttgtccgc gccggggccg ggcggcaccg ccgataagct
     9361 gctggaacgg gccgacgcca tccatgacgg ccataaggcc aagaaagccg cgaagcaggc
     9421 cggggctgtg gccgccgagg gggacgctgc cctgcaccgg ccatccgccc ggctccaact
     9481 gtcggaggaa gaacgggccg acccggattt acagccctat atccgaaaat cggacaaacg
     9541 ggccgataag ctggataagg cccgcgccgc catccccaag aagcgcgtcc cggtcaagga
     9601 aaccgtcttt gacgaggcaa gcggcaaagc aaagaccgtc ctgcactttg acaaacagga
     9661 caaggggcct ccctccctga aacccaatcc ggcaggccgt cctgtttccg aggtttttct
     9721 attcggccac gggaaaatcc atgaagtcga gcatgaaaac gtgggtgtgg agggcggcca
     9781 taagggggag gaacttgccg agcggcaggc cggaaaagcc atccgcagca ccatccggca
     9841 ccacaggctg aaaccctact gggacgcgga aaaggccgag cgcaggctat tgtctgccaa
     9901 cgcggagtat ttctaccaga aatctttgcg ggataacccg gagatcgcac aggcggccag
     9961 caaccccatt tcccggttct ggcagaaaca gcagatcaag cgccagtacg ccaaggccgc
    10021 ccggacagcg ggccagaccg caggcgcggc ggcacagggc gcggccaaga cagccggggc
    10081 ctccacgaaa gcggccaagg ccgcagccaa agaaagtgaa cgggcggcct cctttgtggc
    10141 ccgccactgg aaaggggcgc tggccgtcct tgtgttgggg cttttgatgc tgcttatcat
    10201 cggcggctta cagtcctgca ccgccatgtt cggcagcgcc ggaagcggca tcacggcgtc
    10261 ctcttacttt tccgaggata gcgatatgtt ggccgccgag gacgcctacg cggagctgga
    10321 agccgagctg caagactatc tggacaacta cgaggccacc catgactatg acgaatacca
    10381 ttttgacctt gacgagatca gccatgaccc ctatgtgctg atttccattc tgtcggcgct
    10441 ccatgacggg atatttacca tcggggaggt acagggtgat ttgtccatgc tctttgacaa
    10501 gcaatatatc ctgacagaaa acgtcgtggt agaaacccgt taccgcaccg aaaccgatac
    10561 atggacggac gcggagggca acacccatac agacacctac caagtgccgt atgactatta
    10621 catctgctac gtcacgctgg aaaacttcga cctgtcccat gtgccagtct atatcatgga
    10681 cgaggaaacg ctaagtttct attccctgta tatgtccacg ctaggaaacc ggcctgatct
    10741 gttttccggc agccagtacc ccaacgcctc caccctgacg ccgccgacct actacgacgt
    10801 acccccggag gctttggagg atgccacctt tgccgccatg ctcaaagagg cggaaaaata
    10861 tatcggttat ccctatgtgt ggggcggcag ctctcccagc accagctttg attgcagcgg
    10921 cttcatttcg tgggtaatca atcatagtgg atggaacgtg ggacggctgg gggcgcaggg
    10981 cctatataac atctgcaccc ctgtttccag ttcgcaggcc aagcccggcg accttgtatt
    11041 tttcaaggga acctacgaca cccccggcgt gtcccacgtc ggcctgtatg tgggcaacaa
    11101 tgtgatgctg cattgcggga acccgatttc ctacacgaac cttacttcaa actactggca
    11161 atcccatttt tacagttacg ggcgtttacc ttaacgccag aaaggagctt tcaattatgg
    11221 caaccaaact tgaccgcatc gaaaaggaca tccagaaaac caagtctaag atcgcggagt
    11281 ttcagaaaca gcttcgggag ctggaaacgc agaaaaccga acaggagaat ttgcagatca
    11341 tccagcttgt gcgcggcatg aacatgaagc cggaggaatt tgccgcgttc ttacgcagcg
    11401 gcgcaatgca ggccgcccct gccgcaaccc cttaccatga acaggaggac aatgcccatg
    11461 aagaataaga ccagacggat ttcccgtatt ctccccgcac ttctggccgt gatgctctgc
    11521 gtcacggcct ttcccgttac cgcccttgca ggcggcaatg acccggcacc ggccccgctg
    11581 ccggaggcta caaccgaacc cgctacgggc ggtttggagc ctgaaacaga cgaaaccggc
    11641 accgccacgg acgggacaga aaccgaggcc gaaaaagata ccggccctct gaccggcaag
    11701 gacatcaccg acctgttttc cgcgctgttc ggttccaagg tgagcattgc cgccacggac
    11761 gacggcatcc agatcaccac cggcaccgac aaaaaagaac ctgaacagac cggcaccgtc
    11821 accaccaacg gcggcaggct gaatgtccgc accggcgcgg ggcttgacaa gaccgcgttc
    11881 acccagcttc ccaatgggac gacggttgag gtagtcggca cagacggcga ctggattaaa
    11941 attctcttgc cggagcgcat cggctatgtc cattccgact atatgaccgt cagcgaaaag
    12001 gaagtagcgg ctaccggcga gggcggtttt tccctctcca ttgacccgga ggaaattgct
    12061 tctctgctgg aactgttcaa tggcggcggg gccagcgccg ccctgtcccc ggacgggaac
    12121 ctgtcactga ttgacgacat cggcagcgcc gcgaaaaatg gcaagcagtt tatcaccgtg
    12181 gaaaccaaaa acggcaatgt gttttatctg attattgacc gggacgacga gggaaaagaa
    12241 actgtccatt tcttaaatca ggtggatgaa gccgatctgc tgacgctgat ggaggacggg
    12301 gaaaccgtca cccccgctgc tgtctgctcc tgtaccacga aatgcaaggc cggggctgtg
    12361 aacacgaatt gcccggtatg taaaaccaac ctgaccgagt gcagcggccc ggagccgcag
    12421 gaaccccagc cggaggaacc cgaagccccg gaggaagaac ccaagggcgg cgcgggcggc
    12481 ctgatcgtgt tcctgctggt ggcgctggcg ggcggcggtg cggcccttta ctatttcaag
    12541 ttcaaaaagc ccaaggctga aaccaccggc agcgacgatt tgggcgagta tgattttggg
    12601 gaggacgagg atttggagga cgaggaaccc gaaaccgagt ttgacccgga agctgatttt
    12661 gtgagtgaac aggaggacga ggaatgacat ataaacttgt aatcgcggaa aaacccagcg
    12721 tggcccgcag cattgcgggc gtgatcgggg ccacggagaa acacgacggc tatttggagg
    12781 ggaacggcta tctggtgagc tggtgcatcg gacatctggt gtcctttgcc gacgccggac
    12841 gctatgacga gcgtttcaaa aaatggcgct atgaggattt gcccattctc ccggaaacat
    12901 ggcagtacat cgtccccgac gacaagaaac agcagtttga cgttctctgt tcgctgatgc
    12961 agcgccccga cgtgaccggc cttgtgtgcg ccaccgacgc cgggcgcgag ggggaattga
    13021 ttttccgttt cgtctaccag atggcgggat gccaaaagcc ctttgagcgc ctttggattt
    13081 cctctatgga ggacagtgct attaaggacg gctttgccca cctgaaaccg ggcggcgact
    13141 atgatccgct gtatcaatcg gccctgtgcc gggccaaggc cgactggctg atcggcatta
    13201 acgccacccg gcttttttcc gtcctgtacc acaagaacct gactgtgggc cgcgtccaga
    13261 cgccgacctt aaaaatgctg gtggacaggg acgccaagat taccgatttc aagaaagaga
    13321 aatatcacat cgtccatatc acggcgggcg gcgcggacgc ggtaagcagc cgcttttccg
    13381 acgctgccga ggcaaacacc gtcaaggcgg cttgtgcaaa agcgcaggcc gtttgcgttt
    13441 ccgtgacgcg ggagaaaaag acggaacagc cgcccaagct ctatgacctt accaccttgc
    13501 agcgggaggc taaccggctt ttcggctata ccgccaagca gacccttgac tatgcccagg
    13561 cactgtatct aaagaagctg ctgacctatc cgagaacgga tagtcaatat cttaccgacg
    13621 atatgctgcc cgccgtggaa agccttgtgt ccggcctgtg ggagctggtg ccgtttgcca
    13681 agggcctgaa cctttccccg cagtttgacc gcatcctgaa cagcaaaaag gtgtccgacc
    13741 accacgccat tatccccacc gctgaatttg tgaagcaggg ctttggcact ctggccgaga
    13801 gtgaaaaaaa gctgctgtcc ctgatctgct gcaagctctt atgtgccgtg gccgggccgc
    13861 atatctatga ggccgtcacc gccacattta cctgtgccgg gcaggagttt accgccaagg
    13921 gcaagaccgt cctttccccc ggctggaaag agattgaacg ccggttccgt tcttccttaa
    13981 agaccgacgc cgacgaggac gccgaggcgg tgcgggagct gccggagctt tgcgagggcc
    14041 agacctttgc agacgtggcc gcctctgtca ccgagcattt caccacgccg cccaagccct
    14101 acaccgaaga tacgcttttg tccgctatgg aacgggccgg ggccgaggat atgccggagg
    14161 atgccgagcg caagggatta ggcacaccgg ccacccgcgc cgccattctg gaaaagctgg
    14221 tgcagatggg ctttgtccag agaaaaggca agcagctcat tcccaccaag gacggcatca
    14281 atctggctgt ggtgctgccg gaggcgctta cttctcccca gcttactgcc gaatgggaaa
    14341 accggctgac cgagatcgcc aaggggcagg ccgatcctga cgaatttatg gacggcatcg
    14401 agacgcaggc gcgggagctg gtgcagacct attcctgtat cagtgaggaa aagcagaaat
    14461 tgtttcaggc cgagcgtgtt cctatcggga cgtgtccccg ctgtggtgag gctgtctatg
    14521 agggcaagaa aaactactac tgcggaaacc gggcctgcca gttcgtcatg tggaagaatg
    14581 accgattttt tgaggaacgc aagaaagcgt tcaccccgaa gatcgccgct gcgctgctca
    14641 aaaccggcaa agtcaaggta aagggcctct attccgtaaa gaccggcaaa acctacgacg
    14701 ggacggtgct tttggctgat accgggggca agtacgtcaa ctaccgcatt gaacacagga
    14761 gctaaaggac agtaacacag caagcatagg gagtgtggct acactcccgg taaaatcccc
    14821 cggccgcgtc cctgccgggc cggggatttt tgcttgttgg ggaagtcccc aaccccctat
    14881 tttttgaaac agaacagaga aaggagtgac cgcgtgaacg aagtattttc tatccaagtc
    14941 cataaccggc agtttttgga acagggcaaa caaggcgtct ggctggattt gcccaccaca
    15001 accgaaacgc tgcaagccgc cctgcgggat gttggcatta ctgccgacaa tccgcaggat
    15061 ttttttatca acgggtattc ctgcccggag gacagacacc ttgccattcc ctatgacatg
    15121 gtgctggccg ccgacgtgga cgagctgaat ttccttgccg cccggctggg ccagcttgac
    15181 gccgccgaga tcgcagagct gaacgccgcc ctgcaaaatc cccgtggcgg ctttgagaat
    15241 atcggccaga tcatcgacta caccgaaaat gtggattact atgtgcatct tcccgacgtt
    15301 aggggggatg gacagttggg cgactactac ctgaaccgtt ccggcatggt ggatatgccg
    15361 gaggaatgga aagccgggat tgatacggcg cagtttggac gccacattgc ccagcaggaa
    15421 caaggcgttt ttacccagta cggctatctg gtgagaagcg gcgacgaatg gcagcgcgtc
    15481 catgagggcc agccggtgcc ggaggaatac cgggtgatgc actaccccca gccggaagct
    15541 gaccgggagg aaccgcaggc ccagcagccg gaggcatccg aaagccccga caaagcgccg
    15601 cccccggtta tcccgatcat gctggacggc atgaacagca aagaacgcat gaaagtgatt
    15661 actgaccgtc tggaaaccgg cgtacaggag ctttttgaaa gtagccgcta tatggcttac
    15721 ttatccgcta tgtccaagat gcacgattac agtctgaaca atacgctgct gatcgccatg
    15781 cagggcggtt cgattgtggc gggctataac aggtggcagg atgaatttga ccgccatgtg
    15841 aagcggggcg agaaaggcat taagatactg gccccggccc cgttcaaggt taagcagcag
    15901 atggagaaaa ccgacccggc cacccagcag cccgtgatcg gcgcggacgg caagcccgtg
    15961 accgaagaac aggaagtgac ggttccggcc tttaaggtgg tgacagtctt tgacatcagc
    16021 cagaccgagg gcaaggattt tcccatgccc tatatcagtg aacttaccgg cgacgtgcag
    16081 cagtatcagg atttctttgc cgcgctggaa cggacatcac cctatcccat aagcattgag
    16141 ccgatttcag acggggccaa gggccgctgc ttctacgcaa aacagcgcat tgccatcaac
    16201 gagggcatga gcgaattgca gaccctaaag accgccatcc atgagattgc ccatgccaag
    16261 ctccacgcca ttgaccggga ggcccccgcc gaggaacagg cggcccggcc agaccgcagc
    16321 acccgcgagg tacaggccga aagcgtggcc tataccgtct gccagtatta cgggctggac
    16381 acgtcggact attcttttgg ctatgtggcc gattggagca gcggcaggga gttatccgag
    16441 ctgaaagcct cccttgaaac catccgggcc acggccagcg aattgataac cgagatcgac
    16501 ggccattttg cccagcttca gcaggagcgc gaggcccggc aggaacagga ggccgtcacg
    16561 gttgacgtta ccccggaggc agcggccccg gcgcaggccg aggaagaaca ggccccggcc
    16621 cttgatccgg cagccgagcc gcttgtgact gtgctatgga gcgaaagccc ccagctaaag
    16681 gacggccagc aaatgccgtt gcatgaggcc gacgcgctgt ttgcccgtct ggatgcagca
    16741 tatccggcag aggcaggcta tgacaaaacc gcgttccgca ttgactgtat ctttcagggc
    16801 gagccggaca gctacgaggg gcggcaggat tttggagata gggacggttc cctgatcgaa
    16861 catatccaga gctaccacca attttacgcg caggacgaaa actggaaaaa gcatgtaatc
    16921 gctacaaccg gcatagaggg ctgggagcag gacaaggccc atcgggatat gctgttaggc
    16981 gagtttgtcc cctatatgaa gctgcattgc agccttgccc aaatggaaca gacggcgcag
    17041 ggccgcttac agtccgggga aaccctgacg ccggaggaaa cggcctattt gggggcgctt
    17101 ctctcttatg tgcaggagtg ccgccccatg ctcaatcagg gccagtatca gttgccggag
    17161 ccgccgaaac tggctgactt cgaccagagc ttgcaggact acaaagcaca ggtacaggcc
    17221 gagatcgcgc aggaggccgc cgcagccggt atgaccgtgg aggaatacgc ggcggctggc
    17281 tatgaagcac cccaacagga caccttttcc atctaccagc ttcgaggcgg ggaggaaacc
    17341 cgcgactatc ggtttgagcc atacgagcgt ttacaggccg cagggctggc cgtggacagt
    17401 gccaactatg accttgttta tacggcaccg ctggataaaa gcacgtcttt ggaggatatt
    17461 taccgcacat tcaacatcga ccacccggca gattttaggg gacacagcct ttccgtttcc
    17521 gacgtggtgg tgctgcacca gaatggacag gacaccgccc actatgtaga cagcttcggc
    17581 tatatggaag tgccggagtt tttgcgggaa aatcccctgc gtaccgccga actgtctacg
    17641 gagcagaacg aaaacatgat tgacggcatt atcaacaata cgccgcccga accgcagccg
    17701 gaggaacccg aacagctatc ttttgaggat gtggcagttg cttccggcca gacacaggag
    17761 gaacccccgg aggctgcgcc cccgcaaacg gctgtccgct attatcccat caatgaggga
    17821 gccgcccgcc gcgccaagga agccatgagc ttttctgact accggcccgg cagcgccacg
    17881 gcggaatacc ggcactatgt cgatcaggcc gtggaaattg cggagcgcca gaaaaagcgt
    17941 gtggagccag agtatcacga aaagatagac agcctccttg acacctacgc ccgcaagctg
    18001 gccgagaaca tgaataaggg ctatgagatt accgcccgcg tcccctctgt cctgattgcc
    18061 gggccgtcca atttcccggt acgggcgaaa gaaaagcaga acgccgccag tgaccgcaac
    18121 atggaggaat ttcagtacat tcagggcttg cttgacaaaa tccgcagcac cggcatgggt
    18181 ggtatcagcg ccgacgatcc gcaggccgtt tccaagctgg aaaagaaact ggaaaagctg
    18241 gaagcgtcgc aggagttgat gaaggccgtc aatgcctact accgcaagca tgggacgctg
    18301 gacggatgcc cccatctgat cgaaagaggc attgaaaacc tgaaagcgga tatggcgtcg
    18361 ggctggcact atgagaaaaa accgttccag tcctggcagc tctccaacaa taacgccgag
    18421 atacgccggt taaagggccg cattgaggaa ttgacccgcc aaaaggaggc ggcctatgtg
    18481 ggatgggagt ttgacggcgg cactgtggag atcaaccggg tggccaaccg cctgcaaatc
    18541 ttctttgagg ggaagccgga tgctgctgta cgcgacgagc tgaaaagcaa cggtttccgc
    18601 tggtcgccca aggccggggc atggcagcgg cagttaaacg acaccacaat ccgcgtcgct
    18661 gaccgtatca agtgcatcca gcccctttcc ggcgaaaagc ccagcgcctt acagcttgcg
    18721 gcccgcaggg agaaagaaaa gccctctatc cgggcgcagc tcaacgcggc caagaccgaa
    18781 caggccaagc agccaccggc gcgggaaaaa tcaaagggat tggaggtaga gtaaatgcag
    18841 aagtttgaaa atgtggacgt tctggccgcg ctggaacaga tcatgcggca gaacaccgcg
    18901 ttttatcaaa gtgactttgg cattgacaaa gacattatca ggcgggatgc cgccagcgat
    18961 caggccgcag acaagaccct gctctggatg tcccggccct ccgggacgta ctgtttccgg
    19021 gaacgtgatg ttttcctgaa agacacccga cagtacaaca catggaaatt ttacggggag
    19081 cagacccgtg acaagatact ggcctacgcg gtggagctga ccggcacaca gggcggcact
    19141 atccggggca atctctatga gctggactat ccgcagcatt tccggcacgt cattgaaacc
    19201 gcccagccgg tgagtgtgaa ccggctcttt tatgagcatg gcacccgtga catcccggag
    19261 agccagcgtt ttgacggaag ccctgaccgg gtattgggaa actttttgta ctatgaggcc
    19321 cagccccatg acccggctgt gctgcaggag gcgctacgga cggagcagca cagccggggg
    19381 cgggccgtcc ccggcgattt caaagtccac gtcaccgctt tgcatgacag ccggattgaa
    19441 accgaggccc ggcgtattgt ggacagcctg aaagatctgt ccgcgcctaa cagccccaat
    19501 aaaacgcatt tcatggtgga gatctccccg cactttgcgg cgattgcttc caccaaggat
    19561 aatgaccggc tctttgccat gctgccctat aaatccctgt gttttaccgg gatgaaaggc
    19621 aggcatggcg tgtatgccgt gattaaccgg gacgagcgcc gggatgtgag tattcgcagg
    19681 ccccggccct ctatccgcaa gcagctatcc gacagcaaac aggccgccag cccaaaaaag
    19741 gcggcggcca gaaccaaaaa gaatgaattg gaggtataag gaatgatgca ctttaccgtg
    19801 gaggaagaaa acctgatctg tatgtaccac aatgcagacc ggcgcagaac gataaacagg
    19861 ctcaccgccg tcatgccggt tatggatggg gatatgcggg cgcaggccca gcagaccctt
    19921 tccaagctgg aacgcatgac ggatgccgac tatgacggcc agaagttcca cttcacagac
    19981 gaataacgaa cagcagacgc cgggcgcggc ggccatgtgg tcgcttgcgt ccggcgtctt
    20041 ttctttttgc ctattcctac gcatttagaa cccggtattg tcggggatag gatgaagtat
    20101 tcgcttgccc ctgcaaacat gcgggagaag ccttgcagcg ggcggttttc aatcccttaa
    20161 atgcgtaggt tgcagctata attagacccc tgaaacagat atagccgcgc agcgggtgta
    20221 tctgtttcag gggtgtgcag gtgatagccg caaagcggcg taagagggtg cagccctctt
    20281 gccgtctatc tctgttccag cgggaagctc ggcaatgctt ccagcagttt cagggaaatg
    20341 cgctgccgta tatcttcgtc aatgactttc ttctttttgc cgtttgcctg tctgatctcc
    20401 accgtggaaa gctcgttgat gtagtcagca taatgggcca acactttttc cgtggcccac
    20461 ctctcaccgg caacggcggc tttgatcgtt tcatagtcca gcagcttttc ttgtccgtcc
    20521 atcctgatac ccccgtcaga ctgatttttt ctatgatacc atattcggaa gcatggaacc
    20581 agagaaaacg gcgcttttct gctggtaaat tctacgcatt tagaacagca cttcggcggg
    20641 gataggataa aacacccaca taccccgctg accatgccgg aaaagccttg aaatataagg
    20701 gcttttacag ctctaagtgc gtaggtttcc agtatctttt ccgctggcgc tcggcctctt
    20761 tgcggcggcg catccgtttg gagcaggccg cgcagtattt ggcccggtta gacttcggcg
    20821 tgaacgtggc cccgcactca cagcagcgtt tcacttcgtc ccggtctttg gtgatctcgg
    20881 catacagggc cgcatccagc gggaggacag cggccttaaa ccagcggcac agaagcgaat
    20941 aggaaatgct ctgtacgcaa atacattctt cgccgtcgtc cagcaacagg cagttgccgt
    21001 cacaatagtt gcagcactca tgcgtcagct tccgggcctt gcgatattga gggtaattca
    21061 ttcgtggaat actcatagct cttgcgtcca ttcctttccc ggcgtcatac gcaaaatgcc
    21121 gtccacattc tgcttgatga tgccgtattc ctgcatctgc tttttcgctt cgccgtactt
    21181 ctcatacagc cgttcctttt cctcggccaa tcgcttgtac tcggctttca gggcgtacag
    21241 atcggggagc tttttcacac cctgtccctt tagggccttt gccgcagctt catacaaaat
    21301 caacgtgctt tcatgctcac gccggaattt tgccttatcc gctgcattgc ggtattccat
    21361 agccacgggc cagagctgct tgtaggtaga aatattcttt atcagcaccg ccatatccgc
    21421 aagccggtgt tccatagctt tgagggtagc ggcggcctcg tcgctggcgg cggtgatctc
    21481 ggccactttg ctttccaaat ccgggtaggt gtctatccca tgttcagtca ggaaattcaa
    21541 agtctttgcg gcctgcttca aattgtggag cttcgcccac ctctcatagc ctgccgactg
    21601 ctgggcctta atgttgtttt ccagatcaat ccgcaaagaa atccccttgc gctctttggg
    21661 cgctctggtg ataacgcggc ctttgatacg ctctttgatg gcttcctcgg tgtaggcttc
    21721 ccccaatgtc ttgcagcggg taaagcgttc ctgtccggga gcgcggaaag aaataaactt
    21781 tcccggcttg atctcatagc cctgcgcctg catgagccgc aggaaatcat caaaatcttt
    21841 tgcctgcggg atagcggcgt caatggctgc tttgagcttc gctttccagc tcgtcccttt
    21901 ccgctgggcg tcccactcgg catagctctt gcccttactc tggccgggct tcaccacgga
    21961 caatccgttt tccttgcaca gcctgtcgct ggtgcgccgg atataggcat agctctgttt
    22021 gttggaaatg tacttgcgct gatttaccat atccacggca cagaaaataa tgtgattgtg
    22081 gacgtggccc ttatcaatat gggtagtcag cacataggga tatttccctt gcagcacttc
    22141 ctcggcaagc tgcttcccga tctcatgggc ctgttccggc gtggcttcgc cgggggcaaa
    22201 ggattgtatc aggtggtggc ccagattatt gcccttttgg tatgcctgcg ccagcagcat
    22261 ttcaaattca atgtccgctg tttcataaga gcaggcaaag gaggccacca gcagcttgcc
    22321 gtcagttttg tccgggttct cgatgtagtc aagggctttt ttcagcgttg acttgatagg
    22381 atgtatcttt gtaactgcca tatctgcgcc agcgccccct ttatatcctc gatgtcctgt
    22441 gcgtacaccg gccccatacc gttcagcctc tttgcaatct gattgacgtt gacgccgatc
    22501 ttctgtatct cggcggtctg cgcccggatg tcgctggtgt ccatgttgac gatatagccg
    22561 tcaatgagca tcttccgggc gtaggctgaa aagttcttcg tatgcagcag cgccattttc
    22621 tgttcaatca tagcccgttc ctccggcgtc acagggacgc gcagcacgat ttttcgcttt
    22681 ctgtttgcca tgcttcaccg tcctttccat tcaggggttt ggggatttcc ccaacaagca
    22741 aaacggacgc ggcccggcag ggacggggca ggccgttttt ccgggagtgg ctacactccc
    22801 tatgcttgct acgttactgt ttctccacta acaagacgtt tccaaagccc caaactaccc
    22861 gcaaaatagc cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc tgtgcagctc
    22921 ccgctaaaag taaaacagag gatgccagtt acacatcctc cgtttcattc ttcttcggct
    22981 tttcaattag tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat tcttcggggc
    23041 agagtttcag cttgtggctg accctttgcc gcagttcgct ttcctcccgc atgatctccg
    23101 ggttaaagta gcgttccaca ggaagcccgc aggctttaat aagctgtatg acaaccggca
    23161 gactgggaat ggcccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt
    23221 ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc
    23281 gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt
    23341 gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt
    23401 tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt
    23461 actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg
    23521 ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg
    23581 ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt
    23641 actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat
    23701 ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa
    23761 aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa
    23821 ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc
    23881 agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag
    23941 acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg
    24001 gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt
    24061 cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct
    24121 actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga
    24181 gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa
    24241 agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc
    24301 aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag
    24361 tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt
    24421 taattattat tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga
    24481 aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact
    24541 acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata
    24601 cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct
    24661 gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac
    24721 gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa
    24781 cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg
    24841 gatgacgaaa accaaatccg ggcgtttgat gcagaggcag acgactatgt aacaaagcca
    24901 ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg
    24961 gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt
    25021 gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag
    25081 aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt
    25141 gagggtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa gctgccggaa
    25201 aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata ataatggaaa
    25261 gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta ctgcttgtcg
    25321 gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg atggaattac
    25381 agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc gataggcttg
    25441 atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag ttttatattg
    25501 aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca aatagtttta
    25561 gacccaactt tctttatgtg gtacatagag atgataatat ttcgattgtt gctcaaagca
    25621 aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt atgattgcga
    25681 taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg acaacgccga
    25741 tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc
    25801 tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg tatgtcaggc
    25861 tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa
    25921 cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc gcggctacaa
    25981 gcgttctgtt ggagggaatg cttgaaaata tcggtgacta caaagatcat tctaagtatc
    26041 tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa atactggagc
    26101 ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac atagggcgca
    26161 cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac cagcggttcg
    26221 tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc caaaaggcac
    26281 tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag gtacggatat
    26341 ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc gttcacattg
    26401 atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag gcgcgaagca
    26461 gaaaaagtgg gcgaagcaga aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa
    26521 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    26581 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttgg
    26641 gctctttgtc aaataggact gatagagtga gataagaa
//
LOCUS       DAYYGY010000230         9811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.097, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000230 DAYYGY010000000
VERSION     DAYYGY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.097"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..849
                     /locus_tag="ACHA52_003049"
     CDS             <1..849
                     /locus_tag="ACHA52_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8941655.1"
                     /translation="FFPSIRSLDVWSGKDLLQSEFIKSARQQLANYQLIVFLDYGFDM
                     EMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHN
                     SSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKRGVTTNYFILPLRGNEQ
                     PGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYQFYH
                     SENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKRFFTTDSAIYEQYEYDL
                     SSVKNK"
     gene            complement(992..1933)
                     /locus_tag="ACHA52_003050"
     CDS             complement(992..1933)
                     /locus_tag="ACHA52_003050"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8941656.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2059..3180
                     /locus_tag="ACHA52_003051"
     CDS             2059..3180
                     /locus_tag="ACHA52_003051"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8941657.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3438..4940
                     /gene="eat(A)"
                     /locus_tag="ACHA52_003052"
     CDS             3438..4940
                     /gene="eat(A)"
                     /locus_tag="ACHA52_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8941658.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5007..5534
                     /locus_tag="ACHA52_003053"
     CDS             5007..5534
                     /locus_tag="ACHA52_003053"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8941659.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            5746..6807
                     /locus_tag="ACHA52_003054"
     CDS             5746..6807
                     /locus_tag="ACHA52_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8941660.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6810..7706
                     /gene="murQ"
                     /locus_tag="ACHA52_003055"
     CDS             6810..7706
                     /gene="murQ"
                     /locus_tag="ACHA52_003055"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8941661.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            7720..9702
                     /locus_tag="ACHA52_003056"
     CDS             7720..9702
                     /locus_tag="ACHA52_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8941662.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
BASE COUNT         3204 a         1757 c         2153 g         2697 t
ORIGIN      
        1 ttttttcctt ccatccgctc tttagatgtt tggtccggaa aagatttact acagtcggaa
       61 ttcattaaat cagctcgaca acagttggca aattatcagc tgatcgtttt tttagattat
      121 ggatttgata tggagatggc tcgacgcgtg cgtccattta ccaatgcaaa aatcgtgtta
      181 tttttttgga accactttaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa
      241 ccggcaatcg atgagattta tcattttgat tttttagaag cgaaagaact tggactaaaa
      301 cataatagtt ccttttattc gaaacatatg gatttttctt ctagcgaacc aaagacggat
      361 ctattttttg gtgcaacaaa taacggcaga aaagaacggg cagaaaaata taagcaggaa
      421 tttacaaaaa gaggtgttac gaccaattac tttattcttc cgcttcgagg aaatgaacaa
      481 cctggatacc ttacttatga agaatatctg aaaaaaaccg gggaatcgag ggggattcta
      541 gaactgttgc gggaagggca gcaaggagtg actttgcgaa cgtttgaatc tatgtatttt
      601 caaaagaaac tagtgacaga taatcaagcc atagctcatt atcagtttta tcattctgaa
      661 aatatttttt tgcttcaaga acgtgattta gatgaattac cggaattttt gcatacgcca
      721 tttcaaccaa tcccatcaga actgttggaa ttttttgatg cagaaaattg ggcaaaacgt
      781 tttttcacta ctgactccgc tatttatgaa caatatgaat acgatctttc ctctgttaaa
      841 aataaataaa agtgccaacc aatcatagat gaaaataaac aagcgcaatt cttatggcaa
      901 catgagaatt gtgtttttct ttttgaactt tcatgggggt aaaaaaatga gaaccagctg
      961 ctgatccagc tgattctcat cagagagcct tttattctgc gccaaggatt ccttcttttg
     1021 catggactaa ttgggtcaaa aaggcacaat atggtgtagc aatatcatat ttttgtcctt
     1081 ttcgccaaac ggcaccattg atgtagtcga tttcagtcaa acgatggttt ttgatcaggt
     1141 cttggtacat agatgggtaa tgcaacccga tgttgtcagg atcataagta gaagcaatat
     1201 gttggtacac ttcttcttga tctaatgtga ttccttcttt tgctgcgata gaagcaaact
     1261 cagatacaat cgttcgaacc atgccctctg ccgctttttg cgcacctaat tcagcaatat
     1321 tacaatccaa tatcgtgcat aatccattta atgttccgtt tacacaagct tttcgccaaa
     1381 tggaataacg aacattatga ctatagattg gattcaattt tgcttcttga aagacttcta
     1441 caactttttt tgtaaatgct tcgccttctg gttccaagtt ctctagttcg atttctccat
     1501 cacctaataa tgtgactttt ccgggaccag ctagaccagc tgtccacatc gtgatcccaa
     1561 ataagatatt tttctttggt acatattttt ccagtacatc ttcatgacct aatccattca
     1621 gtaaacacaa aacataagta ttctcagtaa taatggattg gatcgaacga aacatatcat
     1681 ctaattgatt cgcttttgtc aaggctacga tcaaatccac gtgtttgttg ctttcgatga
     1741 tttcttctgg tgaatagatt ggcagttttg caactacttc tttgccatta aagtcagcaa
     1801 tcaatccatt tgtacgaatg gcttcaatgt gagcaggcca gcgatcgatc aagacgacct
     1861 catttccgct ttgatgcaac attaatccga ttcggctgcc cattgctccg gctccagcta
     1921 ttgcgatttt catgtttttt ctctccttta ttcaatgagt tcccttttag tatatagaaa
     1981 aacagaagaa aatagcattg ttttgtttga aaaaagtgaa aaaaagtgtc ttacttaaga
     2041 agaaagtgag ggatatagat ggatattttg gcggcaatcg attcatttaa aggaagtgct
     2101 tcttcatttg aattaaatca ggcagttcta aatggttggg aagaccgtaa aggaagaaaa
     2161 atcaatgtgc cgattgcaga tggcggagaa aatactgcag aagcaatcta ttacgcgctc
     2221 gggggaacct ggcgtacgat tgatgggatg gatctcttat ttcgtcagcg aaaaatcagt
     2281 tatttattga cgaactggca aggaaaaaat accgcagtca ttgaatctgc acaagtatta
     2341 ggattagatt tacttgataa accaacggat caaaccatcc gacttgcttc tagttatgcc
     2401 ttgggagagc ttatcaaaga tacactgctt caagaagtgg aacaaatcat tgtcacttta
     2461 ggaggaagcg gatgtagcga tggtggtcta ggattactcc aatctttggg tgcttcttta
     2521 gaagggtaca cagaaggaaa tcctttgtta tctactagaa aacttcattt ggatcgaagg
     2581 aacgttcttg tttctaaagt aaaagtgatc atcgctgcag acgtgaccaa cccatataca
     2641 ggtcaacaag gagctgcttg gcaatttgga agacaaaaag gaggaacgga tacgacgtta
     2701 gctttcttgg ataaacaagc tgaaaagatt gctcagcaga taaaagatgt ttgggaggtg
     2761 gatttagatg acatcccagg aagcggagca gcaggcggta ttggcggagc cctttttttg
     2821 ttaggagcga agatggtctc tggttttgag ttggtcagcc aaatcactgg attagaaaaa
     2881 cagatcgaac aagcggatgt catcattaca ggagaagggc ggattgatca gcaaacaatc
     2941 cacggaaaag ttccatatgg cgtagcagtg cttgcaaaaa aatatcgaaa accaataatc
     3001 gctatttgcg gaagtagaga ccgagagctg gaaggactct ttgattttct ccccattgtc
     3061 ttaagcattc aattaggacc aatcgattta gaggaagcaa tggaacacag gcaaacatta
     3121 gaaaaaacag aaatcttagg acagacactc agcagatttt tcaacattct cccgaagtag
     3181 ttgacaaaaa caatttcttt atgctatcgt aatttcaaat ataggacaaa gtgagggatc
     3241 cgtgcattga aaaattaagt cgattgatga aagctgcatt cagactgtac cttgtggaga
     3301 caagggagca gtgtgcagat tttcgggatt ggcttggttc tttcgatgta tgggtctttt
     3361 tgtgtgcccc aacccttgca tcctctttag ccagttctga aaataggact ggtctttttt
     3421 tacgaggtga tggaataatg tctaaaatcg aaataaaaaa tctgacattc ggctatgaca
     3481 gccaaggcac attattattt gaacaagcca atctaaactt tgacacacaa tggaaactag
     3541 gacttatcgg acgaaacggt cgaggaaaga caactttact gaatatcctg caaaacaaac
     3601 tcccttatca agggcaagtg atccatcagc aagcgtttgt ctatttcccg cagcaaacaa
     3661 aagataaaga gcgtttgacc tattatgtgt taaatgagat tatggatttt gagatatggg
     3721 aaattgaaag agagctccaa ctgatgcaaa cagatcctga aatcttatgg agagaattca
     3781 gcacactatc aggaggagag aagacaaaag tcctactggc actcttattt gtggatgaca
     3841 ctcatttccc gttaatcgat gaaccaacga atcatttgga tatctctggc agaaaacaag
     3901 tagcggctta cttgaaaaag aaaaaacaag gattcatcgt ggtgagtcat gatcggggat
     3961 ttatcgatga agtagtggac catgttttag caatcgaaaa gagccaactg gaactttatc
     4021 aagggaattt ctctatctat gaagaacaga aaaaacttcg tgatgaattt gaaatagcta
     4081 aaaatgaaaa attgaaaaaa gaagtcagca ggctaaagaa aacagcagct gaaaaagcag
     4141 aatggtctcg ttcccgagaa ggggacaaaa caaagaaaaa agtcggattc atcgatacgg
     4201 aatctaggcg agtgaataaa ggagcagtag gcgctgatgc tgcacggacg atgaaacgat
     4261 ctaaagcaat cgtgaatcgg atggaaaccc agatcagtga gaaagaaaaa ttattaaaag
     4321 atatcgagta tatcgatgct ttgacgatga atagccaagt gtctcatcat aaacggctgt
     4381 taagcgtaga aaatcttcaa ttagggtatg aaaatctgtt attcgagcca gttcatttta
     4441 cgatcgaacc tcatcagcgg atggcaattt caggtcctaa cggcgcggga aagtcatcca
     4501 ttatccatta tcttctgggg acattcaacg gcaaggttgt aggagaaaaa tcccagccca
     4561 aacatttgac cattagttac gtacgccaaa attatgaaga caatcgaggg acattggcgg
     4621 aattcgcgaa gaaaaaccga gtagactatc aagcattttt gaataatctc cgaaagcttg
     4681 ggatggaaag agatgttttt cataataaaa tcgaacagat gagtatggga caacggaaaa
     4741 aagtggaatt ggctaaatct ttatcccaac cagctgagct ctatatatgg gatgaaccat
     4801 tgaattattt ggatgtcttc aatcaagaac aattagaaca actgattttg aacgtgaaac
     4861 ctgctatgtt actagtggaa catgatcaaa cctttctaga taaagtatct actgagatta
     4921 tttctcttga gaaaatctaa accttgtaat ctcaaagaca acaaggtatg gttaaggaga
     4981 actttgtcaa cgaaggagag aatcacatgc tttatgtaac gagacacgga gaaacaactt
     5041 ggaacgcaca aggattagtt tgcgggcgag cagatgtgcc gctgactgaa aaaggacaga
     5101 tgcaggcaca ggcactagca gaaaaggtag tagatttgcc tgttccaatc acgaaaatca
     5161 tccattcccc tcttcaacga gcaagagata cagcacaagc agtagttgat cgtctcaatt
     5221 tgccgatgac cgctgatgag cgattggtag aaatggattt tggggattat gacggactgc
     5281 caagcaaaga tgaaaacttt caaaaagcgc gtttagcgtt tgctgtacgt ttcccaaatg
     5341 gagagtcggt cttagatgtg tacgcacgga tcgttccttt attgaaagaa tgcatggaag
     5401 acgaagaaaa tgtctacttg ttagtctgtc ataatgcctt gataagagtg atcaatgctt
     5461 attttcatcc aatgcctaat gaagggttct ttacattcat ggtagacaat acagaattaa
     5521 tttcttatga gtaattagac ttttgtgtta tttaaataaa atgtttcaag gcactgcttt
     5581 tgcttagcag tgcctttttt gtggctgtgt caattctttt ggcattataa atccaatttt
     5641 tagaaataaa atttaattat tttaacaaaa gtattgaaat tttgtttctt tagatatata
     5701 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     5761 atatttatga atgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     5821 aatggctttg tcggtatttt cacttccatg catataccgg aagatgatgt ttcggcttat
     5881 caaaatcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga gttgatggtc
     5941 gatatttcag gagaggcatt gaaacgagct ggcttttcat tagaagatct aacacccttg
     6001 aaagcaattg gtgtaacagg gttaagaatg gattatcata tttctaatca gcagatcgcc
     6061 gactggtcac atcaattgaa aatcagtttg aatgccagca cgatcacgcc taatgatatc
     6121 gatgagttga aagaagcagc ggctgatttc tcacagattg aagcttggca taattattat
     6181 ccacgtcttg aaacagggtt ggacaaagaa tggtatcagg aaaaaaatca atggctcaaa
     6241 actcaaggat tccttgtaca aggctttgta ccaggagata cagagttgag aggtccactg
     6301 tatcaaggat taccaacttt ggaagaacat cgagggatcc atcctctggc aggtgctctt
     6361 gatctattag caagtaacac ggatattgtt tatatcggag atgcaggtct gtcagaacac
     6421 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     6481 gtagatgaag ttttttacga atatatatta ggaaaacata cgaatcggca agatgatgca
     6541 agagatgtca tacgaagcgc agatgcgcgt tttcgagaga ttccgccaat cccagtaaga
     6601 aatacagcta agcgtatgaa aggatctatt actttagata acgaaaagta tttgcgctat
     6661 atgggtgaga tccaattaac taaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     6721 gccaaagtca tcaaagaaga actaccgctg atcaaccaaa tcaaagctgg tatgaactat
     6781 caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa
     6841 cggaacgcag aaaccaagaa acttttggat tagatgaaat gagtatttct caagcagtcc
     6901 aactcatgaa caaagaagat cagaaagtgg ccgctgctgt tgaaaaagaa ctgccaagta
     6961 tcgagccagt gatcgccaac acgattgaat cctttaaaaa aggtggtcgc ttgatttata
     7021 tgggagctgg aacgagtggt agacttggtg tattagatgc tgctgaatgt gtaccgacat
     7081 tcggtgtaga accggaaatg gtcataggcc taattgctgg tggagaagaa gcaatgactg
     7141 tagccgtcga aggagccgaa gattcttatg aattagggca agaagatcta atcaagctga
     7201 atttaacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga
     7261 ttggtggatt agattacgca aaatcgattg gtgctgttac aggaacgatt tcttgcaata
     7321 aacaagcaga aatcagcgag catgcggatt tcccaatcga agtcgatgca ggaccagaat
     7381 ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaactgatt ttgaatatga
     7441 tttctactat cagtatgatc ggtatcggta aagtattcaa taacttgatg gtcgacgtca
     7501 aaccaacaaa tgaaaaacta attgaacgct caaaacgaat catcatgcaa gcgacagaag
     7561 ctgactacga aacagctgaa aagtactttg aggaagcaga tcaaaatgtc aaactggcta
     7621 tcgtcatgat tttgacaaat agtgataaag aggcagctca agcaaaatta gctgaagcaa
     7681 acggttttgt aaaaggaacc ttatgagggg gaaaagacca tggcaaaaaa cgaattgact
     7741 ttggaagaac tggcaaaaca aatttatcaa ggagcaggcg gtatgggcaa tgttgaaagc
     7801 attgtccact gcatgactcg tgtacgtatg aaggtcatca atgatgatct tgtaaatgtc
     7861 tcggaattaa aagcgattcc aggagtactt ggcgtagtag aggatgaaca gctccaagtc
     7921 atcatcggac ctgggaaagt gaacaaagta gcgcaggaaa tggttgactt agcaggcgtg
     7981 aaattaggag aaaaacttcc ttctgctagt gcaactacag caagttcttc tgcatctgga
     8041 aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa
     8101 ccctctaaaa tcaaatcggt attaaaagac atctctaata tctttgtacc aatgatccct
     8161 gcatttgtcg gcactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca
     8221 gctggaacac tagacgcagg aacatggcaa caatatatcg atgtgatgaa catcttgaaa
     8281 aacgggatgt ttagctatct agttatctat acaggtatca atgctgcaca agtttttggt
     8341 gcaacgccaa cgcttggtgg agttatcggg gctgttgtga tgctgacagg gatgaatcca
     8401 gaagcaccac tgaaaaactt gtttaccggt gaagctcttg caactggaca aggcgggatc
     8461 ttaggtgtta tcttcgcagt ttggttattg tcaatcgttg agaaaaaact gcataaagta
     8521 gtacctgatg cggtagatat catcgttacg ccaactcttt ccctgattgc catcggtctg
     8581 atcgaaattt tcttcatcat gcctttagca gggttcattt ctgacggcat ggtcggtggg
     8641 atcaactggg tacttggtgt tggaggagca tttgccggct tcgttctagg tacattattc
     8701 ttgccaatgg tcatgttcgg actacatcag atcttgacgc caatccatgt acaaatgatt
     8761 gatgaaactg gtcgtacctt attgcttcca atccttgcga tggccggagc aggacaagtg
     8821 ggtgcagcat tggcattatg gatccgctgc aaaaaagaca aagaattaac agaaatgatc
     8881 aaaggggctt taccagtcgg aatcttagga atcggcgaac cattgattta cggagtaaca
     8941 ttacctctag gacgtccatt tattactgct tgtatcggtg gaggtatcgg tggggcagtg
     9001 atcggaatga tcggaaatgt cggagcaatc gctattggac cttcaggagc cgctttgatt
     9061 ccattgatct ctgatggtaa atggttaggc tatatccttg gtttattggc tgcttacgct
     9121 ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa
     9181 ctagcgaccg aaacagccac agtaaatgaa gtacctgaaa aagcagtacc tcaagcttca
     9241 gctgaaagtg tgactatctt atcatcagtt gctgacggaa cagttgtttc tttagataaa
     9301 gcaagtgacc cagtttttgc acaaaaaatg atgggtgaag gttactttgt agaaccagaa
     9361 aacggacata tctattcccc ggttactgga gtaataagtt ctgttttccc aacaaaacat
     9421 gctatcggca ttacgacacc aaatggctta gaaatcttgc ttcacatggg catcaacaca
     9481 gttgacttag gcggtacgcc attcgatgtg aaagtaacag aaggacagca agtgacacct
     9541 gatacgcttg tagcagaagc tgacttagaa gcaatcaaag cagcagacaa agaaacctcc
     9601 atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt
     9661 aaagcgaaag caaaagcacc tgtgatggat gtcaaagctt aataagaata gaaacaatag
     9721 agaacataaa aagaagaagc cgaaatttat ttttcagctt cttcttttgt gttatttatt
     9781 tttttctgcc aaaatttgat aaacgcgtgt c
//
LOCUS       DAYYGY010000231         7959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.109, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000231 DAYYGY010000000
VERSION     DAYYGY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.109"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHA52_003057"
     CDS             128..1564
                     /locus_tag="ACHA52_003057"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8941663.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACHA52_003058"
     CDS             1753..4200
                     /locus_tag="ACHA52_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8941664.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVILLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHA52_003059"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHA52_003059"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8941665.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACHA52_003060"
     CDS             5487..5693
                     /locus_tag="ACHA52_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941666.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACHA52_003061"
     CDS             complement(5690..6793)
                     /locus_tag="ACHA52_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8941667.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHA52_003062"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHA52_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8941668.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2666 a         1358 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgat attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAYYGY010000232         5626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.126, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000232 DAYYGY010000000
VERSION     DAYYGY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.126"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..609
                     /locus_tag="ACHA52_003063"
     CDS             145..609
                     /locus_tag="ACHA52_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8941669.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            593..1222
                     /locus_tag="ACHA52_003064"
     CDS             593..1222
                     /locus_tag="ACHA52_003064"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8941670.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1576..3054
                     /gene="msr(C)"
                     /locus_tag="ACHA52_003065"
     CDS             1576..3054
                     /gene="msr(C)"
                     /locus_tag="ACHA52_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8941671.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3101..3547
                     /locus_tag="ACHA52_003066"
     CDS             3101..3547
                     /locus_tag="ACHA52_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8941672.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3746..4261)
                     /locus_tag="ACHA52_003067"
     CDS             complement(3746..4261)
                     /locus_tag="ACHA52_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8941673.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4378..5580
                     /locus_tag="ACHA52_003068"
     CDS             4378..5580
                     /locus_tag="ACHA52_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8941674.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1750 a          939 c         1198 g         1739 t
ORIGIN      
        1 ttctacaaaa aatttctttt ctctatactt ttctattgag agcgtttttt ttattacaca
       61 gctgattctt tgactttttt ctttttttat tctattattg acaagtcaat cgttgacgag
      121 tcaattatta gaaaggagga aattatgtct ttagaagaga ttagtcacct tttatatcaa
      181 atcaaattag ccgatcaaaa aatcgtccag atctttgaag aaaatatcgg tataagtctt
      241 actagatacg agatcatgat gagactgaaa gaagaaaatc cattgctaca aagcagtttg
      301 caagaaaagt tgcagattga tcaaggagca gttactcgtc atctgaaggt attggaagca
      361 aatggctatg tttcgcgtca gagaaatcct cagaacaacc gggaagtgta tgtagcgcta
      421 acagaaaaag cccagaagaa aatcaagcat tgtgaaatgg accataaaga tacaggttcc
      481 gtgctatccc cggctttcag cgatgtagaa atgaaacaat ttctaacact tttagataaa
      541 ttgaataccc aactttgcaa tataacaaca gaaacgaaag aggttaataa taatggaaca
      601 aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc gttcgcgtat atgatgaaac
      661 atacaaaatc ccacacgaag aaatgctgga aatgatcaaa gaagcgacga ctgcaccttc
      721 ttctgtcaat atgaaaccat ggcgctttgt ggtcgctgaa agcgaggaag caaaagaaat
      781 cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat acttcttctg caatggtcat
      841 gatttttggc gatatgcaat gttacgaata tggcgaagaa atctacaacc aagcttacga
      901 atcagggaag atgccaaaag aagtcagaga tcaacaatta gctgcgatca ttccttatta
      961 caagagtttt tcaagagaag aaatgaacga tgtagtcaaa gtcgattcta gcttagcggc
     1021 tatgcaattc atgttagttg cgcgtgatca tggctatgaa acaaatccaa ttggtggatt
     1081 cgaagcagat cagttggcag aggcatttgg tttggataaa gaccgttatg ttccagttat
     1141 tattttatca ataggaaaag cggtagaaga aggttacgag tctgttcgtt tagcaccaga
     1201 aaagatcact atgttcgaat aaaaggtttt cagttactat tcagtagcta ttgctttttt
     1261 tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa aaatgactgc atcgatgaaa
     1321 ttacgtttcg aacttttgaa taacaactaa ataaattcaa aagacatgta taaacaacaa
     1381 tttcatggat ggagtatgtc cattttttcg aacgacagta gaataggtga aagctatgac
     1441 agatttggtg atttagtcat agttatttcg ttcagagtag gaaaagatgt gatgcagttt
     1501 ttttgcaaac agatttttct gctctttttt agtatcgata attgttgttt tacatgaaat
     1561 aggagtgtaa aacatatgga aaatttagca gtaaatataa caaatctaca agtcagcttt
     1621 ggcaaccagc tagaactatc tattgattct cttcgtgtct atcagcaaga tcgtataggg
     1681 atcataggag aaaatggtgt cggtaaatcg actttgctca aactaatagc cggtgaactt
     1741 tttcccgatc atgggaaaat ccaaacagag atcaccttca actacctgcc tcaattaacc
     1801 tatctcgctg aagcaaagga cctaaatttg gaattagcca gtcatttcca gttaagactg
     1861 gaagaaactt cagagcggaa atggagcgga ggggaagaac gaaagatcga gttgatacgt
     1921 cttctttctt cttatgaaca agggatgctt ctagacgagc caacaaccca tctagataga
     1981 aaaagtattg atcgactgat tgaagagctt cgttattatt atggtacgct ggtttttgtt
     2041 agtcatgatc gctattttct agatgagcta gcatcgaaaa tctgggaagt aaaagacgga
     2101 gaaatccgag agttttcggg gaattatagt gcctatctca ctcaaaagga attggagaaa
     2161 aagactcagc tacgagaagc agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg
     2221 atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag
     2281 caacaaatca gactggatcg gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc
     2341 atccaaaaga atgcgaaaac attagagaga agactccaaa aaataggaga aacaaccaag
     2401 ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact ccacagccgc
     2461 tatcccatca tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt actggtaaat
     2521 ggtgattttc agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt
     2581 aagacaacct tattagaaca tatccgcaaa caaggagaag gaatccttct ctctccgaaa
     2641 gtaagctttc aagtatatca gcaaaagggt tatcaaatga catctgaaga atccatcatt
     2701 cgttttgtca tgagacaaac agagttttca gaatcgcttg tccgtagttt gctgaatcac
     2761 ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg gggagaagcg
     2821 acccgtctga cgattgcttt gctttttact aagccaagta atgtgttgct gttagatgaa
     2881 ccgactaatt ttattgatat ggcaacgatc gaagctttag agaagctaat gcaaatatat
     2941 ccgggaacga ttttgtttac ttctcatgat tcttactttg tcgagcgtac ggctgatgaa
     3001 gtttatgaaa taaaagggca gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc
     3061 tacgatgatt ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa ataaaaaaga
     3121 aggaataatt tttacaacaa tgatgtgttt tttgatggtc ttggggatga gcgcgtataa
     3181 tttatggctg catcaagatc ttcatttatc aagtttgttg attggtctag tgcctggatt
     3241 cgtggtggct ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa aaattgcctt
     3301 taaactacca gtaaatccag aaagcaaggt ccaaatgatc ttaactattt catgcttgat
     3361 ggttttagga atggtgacat gtatgtcttt attcggtgtg atcatggaac aaggaatacc
     3421 tgatcaatta ttctctgctt atatgaatgc ttggaaaatg aatgtgattg ccgctttacc
     3481 gttgcagttg atcatcgttg gcccgctttc aagaagtgtg ctaaaagcaa ttcaaagtaa
     3541 tgaatgatgg aacactgtta tttgaaaaaa ggccttggca tgctttttgt catagtctct
     3601 tgaatgcagt tctagaaaaa tagtgatccg aacgataggt tttggcctgt cgttcggatc
     3661 gctttttctt ttccaactgt attgctcttt tattgaagat aatttgctct tcaatagata
     3721 tttctagaaa ggttctgttt atcccttact caaaaaactg ctgcatttgt tctcttactt
     3781 gctgacacca ttggagttcc tgctgacaaa gcataattct gcgctggatc acaacataac
     3841 gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa ttttttttga tttttttgca
     3901 tgattttgtt cagctgttgt tctctagtga atagcagtcc agtagctgtt gttttatcga
     3961 gcgtcgaaaa agcatagatt tttgctagaa attcatcttt ttgcgtagga gcgggagttt
     4021 cctctttgat ccattcctct acaagagcac gtccttcatc agtcaagtgg tagatctttt
     4081 tttgagagtg ctcgtctttg tccaaaacgg tcacataacc ctgctggtct aacttggcaa
     4141 gcgttgtata gatctgactg tgatgggctt cccaaaaaat tgtgatcagc tgcttcaatt
     4201 catacccaga acgtggtttg gataaaagca cgcataataa tatataggct aaagtattca
     4261 taaaaatcct acctctcgtc ttattttctt tattatatca cagtaaagtt tccacgtttt
     4321 acatgtgcat attgacatgt aaaataaatc atgtatgatg ctaatagaga gaaagggatg
     4381 gaaatattgg agaatcaaat agatatacaa gaacaaacaa atgaagaaac aaaggggaaa
     4441 agttcagcgc tagttgtagc agctacctgt atgatcgctt ttatgggagt gggtctagta
     4501 gacccaatct taaaaacgat tgctgcacag ttaaacgcta cgccagctga aacgaccctg
     4561 ttatttacta gttacatgtt agtcactggt gtggtgatgc tatttacagg tttcttatct
     4621 agtcggatcg gtttgaaaaa aacattgatg acaggattgt ttatcatcgt tgtattcgct
     4681 ggtcttggcg gattatctgg gaatatcgga atgctgattg ggttacgtgc cggctggggt
     4741 gtcgggaatg ctttatttgt ctctactgct ttagcaacga tcgtcagtat tttagttggt
     4801 aacacagaaa aagcaatcat gatgtatgaa gcagcacttg gctgcggaat ggctgttggc
     4861 cctctagtcg gtggggtctt gggcagtggt tcatggcgtt atccattttt tggggtagca
     4921 tttttgatgt tttgcggttt tatagcccta gcggtacttt tacctgaaac tgagaaacca
     4981 aagagaaaag taggcctttg ggaaggtgtc aaagcgttaa gcaatcataa acttcgttca
     5041 gttggactaa tcgcattgtt atataatttc ggattcttta ctttacttgc gtatgcgcca
     5101 tttttattga ttggctattc tgaattagaa gtcggttttg tattttttgg ctggggtgtg
     5161 cttctggcta ttgcttcgat tttcgttgca ccggctcttg agcggaagtt tacgacttat
     5221 cggacaattt tagcagcatt tgtcttattc attatttgtt tgctgttatt aggagctgga
     5281 acttctatgc cagtccttgt accgatcagc attgtgttag caggattttt ccaaggaatt
     5341 atcaatacac tgttgacgac gattgctatg gaaataccag gtctgcaacg aaacgttgct
     5401 tcgtccagct atagttttgt ccgctttttc ggtggagctt tagcaccatt tattgcaggt
     5461 gtgattggtg aacatattgg tgaaagctat gcgttttatt ttgcaggtgc cattgttctt
     5521 atcagtgtat gttgtatcat ttatcaccgt aattattttg ctacaaatga agaagaataa
     5581 gaaatcagag gttgtgaaaa agctgtcctg catcaagtca taagaa
//
LOCUS       DAYYGY010000233         4702 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.134, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000233 DAYYGY010000000
VERSION     DAYYGY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.134"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1000
                     /locus_tag="ACHA52_003069"
     CDS             194..1000
                     /locus_tag="ACHA52_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8941675.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1725
                     /locus_tag="ACHA52_003070"
     CDS             1018..1725
                     /locus_tag="ACHA52_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8941676.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1722..2693
                     /locus_tag="ACHA52_003071"
     CDS             1722..2693
                     /locus_tag="ACHA52_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8941677.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2686..3714
                     /gene="vanB"
                     /locus_tag="ACHA52_003072"
     CDS             2686..3714
                     /gene="vanB"
                     /locus_tag="ACHA52_003072"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8941678.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3720..4328
                     /locus_tag="ACHA52_003073"
     CDS             3720..4328
                     /locus_tag="ACHA52_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8941679.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4394..4567)
                     /locus_tag="ACHA52_003074"
     CDS             complement(4394..4567)
                     /locus_tag="ACHA52_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941680.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1329 a          986 c         1245 g         1142 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg
     1321 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1381 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1441 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1501 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1561 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1621 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1681 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1741 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1801 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1861 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     1921 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     1981 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2041 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2101 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2161 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2221 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2281 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2341 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2401 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2461 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2521 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2581 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2641 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2701 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2761 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2821 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     2881 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     2941 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3001 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     3061 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     3121 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     3181 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
     3241 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
     3301 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
     3361 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
     3421 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
     3481 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
     3541 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
     3601 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
     3661 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
     3721 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
     3781 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
     3841 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
     3901 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
     3961 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
     4021 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
     4081 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
     4141 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
     4201 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
     4261 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
     4321 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
     4381 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
     4441 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
     4501 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
     4561 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
     4621 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
     4681 gttgaactat ttaatggact gt
//
LOCUS       DAYYGY010000234         2202 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.171, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000234 DAYYGY010000000
VERSION     DAYYGY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.171"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..420
                     /locus_tag="ACHA52_003075"
                     /pseudo
     CDS             71..420
                     /locus_tag="ACHA52_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(692..982)
                     /locus_tag="ACHA52_003076"
     CDS             complement(692..982)
                     /locus_tag="ACHA52_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8941681.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1190..1881)
                     /locus_tag="ACHA52_003077"
                     /pseudo
     CDS             complement(1190..1881)
                     /locus_tag="ACHA52_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          634 a          406 c          320 g          842 t
ORIGIN      
        1 cctaatagag tataacatag ctttaagcat tacttaaaga taacttaaaa gttatctttt
       61 aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata gtcaacaaaa
      121 aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat tgcagtaatg
      181 cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa aattgacgaa
      241 caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt cgaaccagga
      301 cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga taatcacctc
      361 ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc cactacctga
      421 gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc gaatactaat
      481 agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag tttggaaagt
      541 ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga ggttcttgtg
      601 agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca aaacaagcgg
      661 ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct tgtcggacct
      721 tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc cctaacacat
      781 gataggatct cacaacaaat tgctttctat tattataata acctgtaacg actaacttca
      841 tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa caattgaccc
      901 catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg atcttatcta
      961 ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa catacattcc
     1021 ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac ctttcttaaa
     1081 atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga ggcagcagta
     1141 tagattactg ttgcttcttt actaatttag gtttacttgt taagaaagat tatttacgca
     1201 aatgttcttc aacaatttgc tgagatttat cagctgtatc ttccacttca tcttttgctt
     1261 tttctaactt ttgaatatat tgtttcaagc ttgagttttc agcagttgct tcgtctaatt
     1321 tcttttgtgt atctgttaac tgatttctac atttgttttt tcttgtgtca gtgttgcgat
     1381 ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt gtgcttcttg
     1441 tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt ggttgatctc
     1501 atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct tgctgactaa
     1561 ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt ctaattcatt
     1621 tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct gttctttttg
     1681 tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg tatgatcaag
     1741 ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca aaataatttc
     1801 atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga caatcccacc
     1861 agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta catgctaaaa
     1921 gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catatgtaat tttaatataa
     1981 atactatcaa atactcattt aatcctcctt ttttatctat ttaaaaaatc ttttttatag
     2041 agaaaaatgc aggttaatac tttcttaaat aaatatttat ggtgaacaaa aacaaaaaag
     2101 caagttttat taaacgtaac ccttttcatt tcatagatta ctgtatttat tacaaaaaac
     2161 gcggatttgt cataaattgt catgcttctt ttacctatga aa
//
LOCUS       DAYYGY010000235         1532 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.190, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000235 DAYYGY010000000
VERSION     DAYYGY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.190"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1103
                     /locus_tag="ACHA52_003078"
                     /note="aph(2'')-Ia"
     CDS             <1..1103
                     /locus_tag="ACHA52_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFM8941682.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE
                     YILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLT
                     GIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEY
                     YPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1233..>1532)
                     /locus_tag="ACHA52_003079"
     CDS             complement(1233..>1532)
                     /locus_tag="ACHA52_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8941683.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          610 a          144 c          259 g          519 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1201 gtgtaaaatg ctatacaatg tttttaccat ttctacttat caaaattgat gtattttctt
     1261 gaagaataaa tccattcatc atgtaggtcc ataagaacgg ctccaattaa gcgattggct
     1321 gatgtttgat tggggaagat gcgaataatc ttttctcttc tgcgtacttc ttgattcagt
     1381 cgttcaatta gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga
     1441 aaggcgtctt cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttggttc
     1501 tgttgcaaag ttttaaatct actatcaaat aa
//
LOCUS       DAYYGY010000236         1522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.192, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000236 DAYYGY010000000
VERSION     DAYYGY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.192"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..157
                     /locus_tag="ACHA52_003080"
     CDS             <1..157
                     /locus_tag="ACHA52_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8941684.1"
                     /translation="KEYSKEIGCSEMFLITDKGNPRACHVYEKLGGKNDYKDEIVYVY
                     DYEKGDK"
     gene            158..>1522
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA52_003081"
     CDS             158..>1522
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA52_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFM8941685.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          636 a          111 c          266 g          509 t
ORIGIN      
        1 taaggaatat tctaaagaga ttggttgttc tgaaatgttt ttaataactg ataaaggtaa
       61 tcctagagct tgccatgtat atgaaaaatt aggtggtaaa aatgattata aagatgaaat
      121 agtatatgta tatgattatg aaaaaggtga taaataaatg aatatagttg aaaatgaaat
      181 atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt taactgatga
      241 aagagtatta gaattttatg atggtagaga taaaaaatat acattagaat cattaaaaaa
      301 acattataca gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt
      361 tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta
      421 tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg
      481 gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga aaaaagaaag
      541 aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa taagggcata
      601 ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac acgagggcaa
      661 aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa atgttaaggc
      721 aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat
      781 cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa
      841 atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa
      901 tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga
      961 attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag aaatttattc
     1021 tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt ttttaagaca
     1081 aatgcacggt ttagattata cagatattag tgaatgtact attgataata aacaaaatgt
     1141 attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg atatagaaaa
     1201 agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg agggtaaaaa
     1261 gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca ataatagatt
     1321 aactggaata attgattttg gagattctgg aattatagat gaatattgtg attttatata
     1381 cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata tattaagaat
     1441 gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg aagaatatta
     1501 tcctattgaa actattgttt at
//
LOCUS       DAYYGY010000237         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.193, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000237 DAYYGY010000000
VERSION     DAYYGY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.193"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACHA52_003082"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACHA52_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACHA52_003083"
     CDS             394..477
                     /locus_tag="ACHA52_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM8941686.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHA52_003084"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHA52_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8941687.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAYYGY010000238         1349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8123
            SAMEA115709532-rid22597313.guided.196, whole genome shotgun
            sequence.
ACCESSION   DAYYGY010000238 DAYYGY010000000
VERSION     DAYYGY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709532
            Sequence Read Archive: ERR13199250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290481.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:27:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,021
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 45 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 24 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709532-rid22597313.guided.196"
                     /isolate="VRE8123"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..297
                     /locus_tag="ACHA52_003085"
                     /pseudo
     CDS             46..297
                     /locus_tag="ACHA52_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="ACHA52_003086"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="ACHA52_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8941688.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
